MD01G1071500.v1.1

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013141
Physical Location & Seq
Forward (+)
17661763 .. 17666232
4470 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1071500.v1.1.491

Sequence Viewer

Length: 1548 bp
ATGCTGGCAACTCACGCGCCACCCTCATTGATAACCTCACTCCTCTGCCTTCCTTCTCCCCCTCTCTCCTCTCTTCGCACGCTTCCACTATTTCACATCGCCAATTTTCGCTGCTTCCGTTATAGCTGGAGCTCACTCCGTATGGTAACAGCCATGTCTGTACAGACCGCCGCGTCCGCCGTATCATCGCCCGACCACGCTGCCGGGAAATGGTTTTCGGTGCCGGAGCTCCGACTCCGAGACCACCGATTCACTGTGCCTCTCGACTACTCCGTCGATCGTACCGCTTCGCCTAAGATCTCAGTCTTCGCTCGCGAAGTTGTTTCAGTTGGTAAAGAAGAACAACAATTGCCATACCTATTATATCTACAAGGTGGACCTGGATTTGAGGCCCCCCGACCAACTGAATCCAGTGGATGGATACGTAAAGCATGTGAAGAATTTCGTGTTATATTGCTTGATCAGCGAGGAACAGGTTTATCAACTCCTTTGACGGCTTCATCTTTGTTGCAATTGAAGTCTGAAGAGAAATTGGCTGATTATGTAAAACATTTTCGAGCTGATAATATAGTAAATGATGCAGAGTTTATTCGAGTACGTCTTGTTCCTGATGCTGCGCCATGGACAATCTTGGGTCAGAGCTTTGGTGGTTTTTGTGCAGTAACTTATTTGAGTTTTGCACCACAAGGACTAAAGCAAGTCCTTTTAACTGGAGGAATCCCTCCAATAGGAAATGGATTCACTGGAGACGTTGTATATAGAGCATGCTCTGAACAGATTATAATTCAGAATGAGAAGTACTACAAAAGGTATCCTGAAGATATTGAGGTTGTTCGTGAAGTTGTTAACTATCTATCAGAATCAGAGGGCGGTGGGGTCCCACTTCCATCCGGGGGCTTCCTAACCCCAAAGGGATTGCAAACTCTTGGTCTTAGTGGCTTAGGATCCAGTGCTGGTTTTGAGCGCTTACATTACATGTTTGAGAGGGCTTGGGATCCTATAATAGTTCCAGGAGCACGAAAGGAAATTAGTTATTACTTCTTGGATGCTTTTGACAAGTGGTCTTCTTTTGATACAAATCCACTTTTTGCTCTTCTTCATGAGTCCATATACTGTCAGGGTGCTTCATCACGGTGGTCTGCTCATAGAATAAGGGCTGAAGATGAGGGCAAATTTGATGCAGTCCGGGCTGCAAAAGAAGGTCGTCCCGTACTTTTCACAGGAGAGATGATCTTCCCATGGATGTTCGATGAGATTCATGCCTTAAGGAAATTCAAAGGTGCTTCTCATGTATTGGCTGAGAAGAAGGATTGGCCTCCCCTATATAACATCGATGCACTAAAAAATAACAAGGTACCTGTTGCTGCTGCTGTTTACTACGAAGATATGTATGTCAACTTCAAGCTGGCCATGGAAACTGCTTCGCAGATAGCAGGGATTCGGTTGTGGATAACCAACGAATTCATGCATTCTGGTCTACGCGATGCAGGGAGCCAGGTTTTTGATCATTTGATGGGAATGCTAGATGGAAAGAAGCCTTTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

516

Amino Acids

57.5

Weight (kDa)

6.88

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 120 - 277 2.6e-12 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61540
fragaria_vesca FvH4_7g15410 FvH4_7g15410 FvH4_7g15410
malus_domestica MD01G1071500.v1.1
prunus_persica Prupe.2G177700_v2.0.a1
pyrus_communis pycom01g10070
rosa_chinensis RchiOBHm_Chr1g0357731
rosa_laevigata RLG00000028002
rosa_multiflora Rmu_ssc0000399.1_g000023
rosa_roxburghii Rroxscaffold_4G00298570
rosa_rugosa Rorug01G0258000 Rorug01G0258100
rosa_samantha Rh1AG272000 Rh1BG239200 Rh1CG255900 Rh1DG267500
rosa_wichuraiana Rw1G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 782
AasI GACNNNNNNGTC 2 cut(s) 172, 272
Acc65I GGTACC 1 cut(s) 1354
AccB1I GGYRCC 2 cut(s) 220, 1354
AccB7I CCANNNNNTGG 1 cut(s) 417
AccI GTMKAC 1 cut(s) 1477
AccII CGCG 4 cut(s) 17, 173, 315, 1482
AciI CCGC 5 cut(s) 168, 171, 177, 285, 870
AclWI GGATC 4 cut(s) 939, 952, 989, 1002
AcoI YGGCCR 1 cut(s) 1407
AcsI RAATTY 4 cut(s) 440, 1172, 1271, 1460
AcuI CTGAAG 3 cut(s) 543, 837, 1179
AfaI GTAC 6 cut(s) 162, 283, 597, 800, 1212, 1356
AfeI AGCGCT 1 cut(s) 965
AfiI CCNNNNNNNGG 4 cut(s) 210, 417, 728, 893
AflII CTTAAG 1 cut(s) 1264
AflIII ACRYGT 1 cut(s) 975
AgsI TTSAA 3 cut(s) 517, 1276, 1402
AhdI GACNNNNNGTC 1 cut(s) 1060
AjnI CCWGG 3 cut(s) 379, 1009, 1494
AluBI AGCT 6 cut(s) 126, 132, 229, 560, 642, 1405
AluI AGCT 6 cut(s) 126, 132, 229, 560, 642, 1405
Alw21I GWGCWC 3 cut(s) 134, 231, 1018
Alw26I GTCTC 2 cut(s) 234, 741
AlwI GGATC 4 cut(s) 939, 952, 989, 1002
Aor51HI AGCGCT 1 cut(s) 965
AoxI GGCC 3 cut(s) 390, 1313, 1407
ApeKI GCWGC 6 cut(s) 111, 200, 614, 1190, 1364, 1367
ApoI RAATTY 4 cut(s) 440, 1172, 1271, 1460
ArsI GACNNNNNNTTYG 2 cut(s) 913, 945
Asp700I GAANNNNTTC 1 cut(s) 441
Asp718I GGTACC 1 cut(s) 1354
AspLEI GCGC 3 cut(s) 19, 619, 966
AspS9I GGNCC 3 cut(s) 377, 391, 877
AsuC2I CCSGG 3 cut(s) 205, 892, 1187
AvaII GGWCC 2 cut(s) 377, 877
BalI TGGCCA 1 cut(s) 1409
BamHI GGATCC 2 cut(s) 944, 994
BanI GGYRCC 2 cut(s) 220, 1354
BanII GRGCYC 2 cut(s) 134, 231
BarI GAAGNNNNNNTAC 2 cut(s) 1382, 1414
BbsI GAAGAC 2 cut(s) 298, 1056
Bbv12I GWGCWC 3 cut(s) 134, 231, 1018
BbvI GCAGC 6 cut(s) 98, 187, 601, 1177, 1351, 1354
BccI CCATC 4 cut(s) 411, 895, 1507, 1520
BceAI ACGGC 2 cut(s) 164, 510
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 3 cut(s) 381, 1011, 1496
BciVI GTATCC 2 cut(s) 414, 822
BclI TGATCA 2 cut(s) 460, 1504
BcnI CCSGG 3 cut(s) 205, 892, 1187
BcoDI GTCTC 2 cut(s) 234, 741
BfaI CTAG 1 cut(s) 1523
BfoI RGCGCY 1 cut(s) 967
BfrI CTTAAG 1 cut(s) 1264
BfuI GTATCC 2 cut(s) 414, 822
BglII AGATCT 1 cut(s) 297
BisI GCNGC 7 cut(s) 112, 171, 201, 615, 1191, 1365, 1368
BlsI GCNGC 7 cut(s) 113, 172, 202, 616, 1192, 1366, 1369
BmcAI AGTACT 1 cut(s) 800
Bme1390I CCNGG 6 cut(s) 205, 381, 892, 1011, 1187, 1496
Bme18I GGWCC 2 cut(s) 377, 877
BmeRI GACNNNNNGTC 1 cut(s) 1060
BmgT120I GGNCC 3 cut(s) 377, 391, 877
BmiI GGNNCC 8 cut(s) 222, 393, 878, 879, 946, 996, 1356, 1493
BmrFI CCNGG 6 cut(s) 205, 381, 892, 1011, 1187, 1496
BmsI GCATC 6 cut(s) 568, 601, 1036, 1168, 1324, 1474
BpiI GAAGAC 2 cut(s) 298, 1056
BpmI CTGGAG 3 cut(s) 148, 732, 765
Bpu10I CCTNAGC 1 cut(s) 940
BpuMI CCSGG 3 cut(s) 205, 892, 1187
Bsa29I ATCGAT 1 cut(s) 1332
BsaAI YACGTR 1 cut(s) 425
BsaBI GATNNNNATC 1 cut(s) 1077
BsaI GGTCTC 1 cut(s) 234
BsaJI CCNNGG 4 cut(s) 620, 891, 1238, 1410
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 4 cut(s) 210, 417, 728, 893
Bse1I ACTGG 4 cut(s) 411, 715, 748, 948
Bse8I GATNNNNATC 1 cut(s) 1077
BseBI CCWGG 3 cut(s) 381, 1011, 1496
BseCI ATCGAT 1 cut(s) 1332
BseDI CCNNGG 4 cut(s) 620, 891, 1238, 1410
BseGI GGATG 4 cut(s) 422, 887, 1051, 1248
BseJI GATNNNNATC 1 cut(s) 1077
BseLI CCNNNNNNNGG 4 cut(s) 210, 417, 728, 893
BseMII CTCAG 2 cut(s) 315, 1290
BseNI ACTGG 4 cut(s) 411, 715, 748, 948
BseRI GAGGAG 2 cut(s) 32, 58
BseXI GCAGC 6 cut(s) 98, 187, 601, 1177, 1351, 1354
BsgI GTGCAG 1 cut(s) 678
Bsh1236I CGCG 4 cut(s) 17, 173, 315, 1482
Bsh1285I CGRYCG 1 cut(s) 280
BshFI GGCC 3 cut(s) 392, 1315, 1409
BshNI GGYRCC 2 cut(s) 220, 1354
BshVI ATCGAT 1 cut(s) 1332
BsiEI CGRYCG 1 cut(s) 280
BsiHKAI GWGCWC 3 cut(s) 134, 231, 1018
BsiSI CCGG 4 cut(s) 204, 224, 891, 1186
BslFI GGGAC 2 cut(s) 863, 1191
BslI CCNNNNNNNGG 4 cut(s) 210, 417, 728, 893
BsmAI GTCTC 2 cut(s) 234, 741
BsmBI CGTCTC 1 cut(s) 741
BsmFI GGGAC 2 cut(s) 863, 1191
BsmI GAATGC 2 cut(s) 1468, 1524
BsnI GGCC 3 cut(s) 392, 1315, 1409
Bso31I GGTCTC 1 cut(s) 234
Bsp1286I GDGCHC 3 cut(s) 134, 231, 1018
Bsp1407I TGTACA 1 cut(s) 160
Bsp143I GATC 7 cut(s) 277, 297, 460, 944, 994, 1230, 1504
Bsp19I CCATGG 3 cut(s) 620, 1238, 1410
Bsp68I TCGCGA 1 cut(s) 315
BspACI CCGC 5 cut(s) 168, 171, 177, 285, 870
BspANI GGCC 3 cut(s) 392, 1315, 1409
BspCNI CTCAG 2 cut(s) 314, 1291
BspDI ATCGAT 1 cut(s) 1332
BspFNI CGCG 4 cut(s) 17, 173, 315, 1482
BspHI TCATGA 1 cut(s) 1099
BspLI GGNNCC 8 cut(s) 222, 393, 878, 879, 946, 996, 1356, 1493
BspPI GGATC 4 cut(s) 939, 952, 989, 1002
BspQI GCTCTTC 1 cut(s) 1098
BspT107I GGYRCC 2 cut(s) 220, 1354
BspTI CTTAAG 1 cut(s) 1264
BspTNI GGTCTC 1 cut(s) 234
BsrGI TGTACA 1 cut(s) 160
BsrI ACTGG 4 cut(s) 411, 715, 748, 948
BssECI CCNNGG 4 cut(s) 620, 891, 1238, 1410
BssMI GATC 7 cut(s) 277, 297, 460, 944, 994, 1230, 1504
BssT1I CCWWGG 3 cut(s) 620, 1238, 1410
Bst2UI CCWGG 3 cut(s) 381, 1011, 1496
Bst4CI ACNGT 3 cut(s) 256, 1115, 1134
Bst6I CTCTTC 3 cut(s) 78, 519, 1098
BstAFI CTTAAG 1 cut(s) 1264
BstAUI TGTACA 1 cut(s) 160
BstBAI YACGTR 1 cut(s) 425
BstC8I GCNNGC 5 cut(s) 6, 80, 313, 766, 1407
BstDEI CTNAG 5 cut(s) 294, 301, 932, 940, 1299
BstDSI CCRYGG 3 cut(s) 620, 1238, 1410
BstENI CCTNNNNNAGG 1 cut(s) 726
BstF5I GGATG 4 cut(s) 422, 887, 1051, 1248
BstFNI CGCG 4 cut(s) 17, 173, 315, 1482
BstH2I RGCGCY 1 cut(s) 967
BstHHI GCGC 3 cut(s) 19, 619, 966
BstKTI GATC 7 cut(s) 280, 300, 463, 947, 997, 1233, 1507
BstMAI GTCTC 2 cut(s) 234, 741
BstMBI GATC 7 cut(s) 277, 297, 460, 944, 994, 1230, 1504
BstMCI CGRYCG 1 cut(s) 280
BstMWI GCNNNNNNNGC 4 cut(s) 14, 176, 463, 1187
BstNI CCWGG 3 cut(s) 381, 1011, 1496
BstNSI RCATGY 3 cut(s) 435, 768, 979
BstSCI CCNGG 6 cut(s) 203, 379, 890, 1009, 1185, 1494
BstSNI TACGTA 1 cut(s) 425
BstUI CGCG 4 cut(s) 17, 173, 315, 1482
BstV1I GCAGC 6 cut(s) 98, 187, 601, 1177, 1351, 1354
BstV2I GAAGAC 2 cut(s) 298, 1056
BstX2I RGATCY 3 cut(s) 297, 944, 994
BstYI RGATCY 3 cut(s) 297, 944, 994
Bsu15I ATCGAT 1 cut(s) 1332
BsuI GTATCC 2 cut(s) 414, 822
BsuRI GGCC 3 cut(s) 392, 1315, 1409
BsuTUI ATCGAT 1 cut(s) 1332
BtgI CCRYGG 3 cut(s) 620, 1238, 1410
BtgZI GCGATG 3 cut(s) 82, 171, 1497
BtsCI GGATG 4 cut(s) 422, 887, 1051, 1248
BtsIMutI CAGTG 4 cut(s) 252, 418, 741, 955
BtuMI TCGCGA 1 cut(s) 315
Cac8I GCNNGC 5 cut(s) 6, 80, 313, 766, 1407
CciI TCATGA 1 cut(s) 1099
CfoI GCGC 3 cut(s) 19, 619, 966
Cfr13I GGNCC 3 cut(s) 377, 391, 877
ClaI ATCGAT 1 cut(s) 1332
CseI GACGC 1 cut(s) 162
Csp6I GTAC 6 cut(s) 161, 282, 596, 799, 1211, 1355
CspCI CAANNNNNGTGG 2 cut(s) 9, 44
CviQI GTAC 6 cut(s) 161, 282, 596, 799, 1211, 1355
DdeI CTNAG 5 cut(s) 294, 301, 932, 940, 1299
DpnI GATC 7 cut(s) 279, 299, 462, 946, 996, 1232, 1506
DpnII GATC 7 cut(s) 277, 297, 460, 944, 994, 1230, 1504
DrdI GACNNNNNNGTC 2 cut(s) 172, 272
DriI GACNNNNNGTC 1 cut(s) 1060
DseDI GACNNNNNNGTC 2 cut(s) 172, 272
EaeI YGGCCR 1 cut(s) 1407
Eam1104I CTCTTC 3 cut(s) 78, 519, 1098
Eam1105I GACNNNNNGTC 1 cut(s) 1060
EarI CTCTTC 3 cut(s) 78, 519, 1098
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 2 cut(s) 132, 229
Eco105I TACGTA 1 cut(s) 425
Eco130I CCWWGG 3 cut(s) 620, 1238, 1410
Eco24I GRGCYC 2 cut(s) 134, 231
Eco31I GGTCTC 1 cut(s) 234
Eco47I GGWCC 2 cut(s) 377, 877
Eco47III AGCGCT 1 cut(s) 965
Eco53kI GAGCTC 2 cut(s) 132, 229
Eco57I CTGAAG 3 cut(s) 543, 837, 1179
EcoICRI GAGCTC 2 cut(s) 132, 229
EcoNI CCTNNNNNAGG 1 cut(s) 726
EcoO109I RGGNCCY 2 cut(s) 391, 877
EcoRI GAATTC 1 cut(s) 1460
EcoRII CCWGG 3 cut(s) 379, 1009, 1494
EcoT14I CCWWGG 3 cut(s) 620, 1238, 1410
EcoT22I ATGCAT 1 cut(s) 1470
EcoT38I GRGCYC 2 cut(s) 134, 231
ErhI CCWWGG 3 cut(s) 620, 1238, 1410
Esp3I CGTCTC 1 cut(s) 741
FaqI GGGAC 2 cut(s) 863, 1191
FbaI TGATCA 2 cut(s) 460, 1504
FblI GTMKAC 1 cut(s) 1477
Fnu4HI GCNGC 7 cut(s) 112, 171, 201, 615, 1191, 1365, 1368
FokI GGATG 4 cut(s) 429, 874, 1058, 1255
FriOI GRGCYC 2 cut(s) 134, 231
Fsp4HI GCNGC 7 cut(s) 112, 171, 201, 615, 1191, 1365, 1368
FspBI CTAG 1 cut(s) 1523
GlaI GCGC 3 cut(s) 18, 618, 965
GluI GCNGC 7 cut(s) 112, 171, 201, 615, 1191, 1365, 1368
GsuI CTGGAG 3 cut(s) 148, 732, 765
HaeII RGCGCY 1 cut(s) 967
HaeIII GGCC 3 cut(s) 392, 1315, 1409
HapII CCGG 4 cut(s) 204, 224, 891, 1186
HgaI GACGC 1 cut(s) 162
HhaI GCGC 3 cut(s) 19, 619, 966
Hin6I GCGC 3 cut(s) 17, 617, 964
HinP1I GCGC 3 cut(s) 17, 617, 964
HincII GTYRAC 2 cut(s) 847, 1396
HindII GTYRAC 2 cut(s) 847, 1396
HinfI GANTC 9 cut(s) 234, 249, 407, 717, 738, 860, 1103, 1255, 1438
HpaI GTTAAC 1 cut(s) 847
HpaII CCGG 4 cut(s) 204, 224, 891, 1186
Hpy166II GTNNAC 5 cut(s) 377, 847, 1375, 1396, 1478
Hpy188I TCNGA 9 cut(s) 233, 239, 523, 639, 772, 789, 859, 865, 1547
Hpy188III TCNNGA 6 cut(s) 263, 314, 608, 815, 836, 1100
Hpy8I GTNNAC 5 cut(s) 377, 847, 1375, 1396, 1478
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 4 cut(s) 59, 63, 1193, 1300
HpyCH4III ACNGT 3 cut(s) 256, 1115, 1134
HpyCH4IV ACGT 3 cut(s) 424, 598, 750
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 176, 463, 1187
HpyF3I CTNAG 5 cut(s) 294, 301, 932, 940, 1299
HpySE526I ACGT 3 cut(s) 424, 598, 750
HspAI GCGC 3 cut(s) 17, 617, 964
KflI GGGWCCC 1 cut(s) 877
KpnI GGTACC 1 cut(s) 1358
Ksp22I TGATCA 2 cut(s) 460, 1504
KspAI GTTAAC 1 cut(s) 847
Kzo9I GATC 7 cut(s) 277, 297, 460, 944, 994, 1230, 1504
LguI GCTCTTC 1 cut(s) 1098
LmnI GCTCC 5 cut(s) 129, 226, 234, 1013, 1491
Lsp1109I GCAGC 6 cut(s) 98, 187, 601, 1177, 1351, 1354
LweI GCATC 6 cut(s) 568, 601, 1036, 1168, 1324, 1474
MaeI CTAG 1 cut(s) 1523
MaeII ACGT 3 cut(s) 424, 598, 750
MaeIII GTNAC 2 cut(s) 145, 661
MalI GATC 7 cut(s) 279, 299, 462, 946, 996, 1232, 1506
MboI GATC 7 cut(s) 277, 297, 460, 944, 994, 1230, 1504
MfeI CAATTG 2 cut(s) 347, 512
MflI RGATCY 3 cut(s) 297, 944, 994
MhlI GDGCHC 3 cut(s) 134, 231, 1018
MlsI TGGCCA 1 cut(s) 1409
MluNI TGGCCA 1 cut(s) 1409
MlyI GAGTC 2 cut(s) 228, 1112
MmeI TCCRAC 1 cut(s) 256
Mox20I TGGCCA 1 cut(s) 1409
Mph1103I ATGCAT 1 cut(s) 1470
MroXI GAANNNNTTC 1 cut(s) 441
MscI TGGCCA 1 cut(s) 1409
MseI TTAA 3 cut(s) 707, 846, 1265
MslI CAYNNNNRTG 1 cut(s) 1132
Msp20I TGGCCA 1 cut(s) 1409
MspCI CTTAAG 1 cut(s) 1264
MspI CCGG 4 cut(s) 204, 224, 891, 1186
MspR9I CCNGG 6 cut(s) 205, 381, 892, 1011, 1187, 1496
MunI CAATTG 2 cut(s) 347, 512
Mva1269I GAATGC 2 cut(s) 1468, 1524
MvaI CCWGG 3 cut(s) 381, 1011, 1496
MvnI CGCG 4 cut(s) 17, 173, 315, 1482
MwoI GCNNNNNNNGC 4 cut(s) 14, 176, 463, 1187
NciI CCSGG 3 cut(s) 205, 892, 1187
NcoI CCATGG 3 cut(s) 620, 1238, 1410
NdeII GATC 7 cut(s) 277, 297, 460, 944, 994, 1230, 1504
NlaIV GGNNCC 8 cut(s) 222, 393, 878, 879, 946, 996, 1356, 1493
NruI TCGCGA 1 cut(s) 315
NsiI ATGCAT 1 cut(s) 1470
NspI RCATGY 3 cut(s) 435, 768, 979
PaeI GCATGC 1 cut(s) 768
PagI TCATGA 1 cut(s) 1099
PciI ACATGT 1 cut(s) 975
PciSI GCTCTTC 1 cut(s) 1098
PcsI WCGNNNNNNNCGW 2 cut(s) 115, 270
PctI GAATGC 2 cut(s) 1468, 1524
PdmI GAANNNNTTC 1 cut(s) 441
PfeI GAWTC 7 cut(s) 249, 407, 717, 738, 860, 1255, 1438
PflMI CCANNNNNTGG 1 cut(s) 417
PfoI TCCNGGA 1 cut(s) 1009
PkrI GCNGC 7 cut(s) 113, 172, 202, 616, 1192, 1366, 1369
Ple19I CGATCG 1 cut(s) 280
PleI GAGTC 2 cut(s) 228, 1111
PpsI GAGTC 2 cut(s) 228, 1111
Ppu21I YACGTR 1 cut(s) 425
PpuMI RGGWCCY 1 cut(s) 877
PscI ACATGT 1 cut(s) 975
PsiI TTATAA 1 cut(s) 782
Psp124BI GAGCTC 2 cut(s) 134, 231
Psp5II RGGWCCY 1 cut(s) 877
Psp6I CCWGG 3 cut(s) 379, 1009, 1494
PspGI CCWGG 3 cut(s) 379, 1009, 1494
PspN4I GGNNCC 8 cut(s) 222, 393, 878, 879, 946, 996, 1356, 1493
PspPI GGNCC 3 cut(s) 377, 391, 877
PspPPI RGGWCCY 1 cut(s) 877
PsrI GAACNNNNNNTAC 2 cut(s) 588, 620
PsuI RGATCY 3 cut(s) 297, 944, 994
PvuI CGATCG 1 cut(s) 280
RruI TCGCGA 1 cut(s) 315
RsaI GTAC 6 cut(s) 162, 283, 597, 800, 1212, 1356
RsaNI GTAC 6 cut(s) 161, 282, 596, 799, 1211, 1355
RseI CAYNNNNRTG 1 cut(s) 1132
SacI GAGCTC 2 cut(s) 134, 231
SapI GCTCTTC 1 cut(s) 1098
SaqAI TTAA 3 cut(s) 707, 846, 1265
SatI GCNGC 7 cut(s) 112, 171, 201, 615, 1191, 1365, 1368
Sau3AI GATC 7 cut(s) 277, 297, 460, 944, 994, 1230, 1504
Sau96I GGNCC 3 cut(s) 377, 391, 877
ScaI AGTACT 1 cut(s) 800
SchI GAGTC 2 cut(s) 228, 1112
ScrFI CCNGG 6 cut(s) 205, 381, 892, 1011, 1187, 1496
SduI GDGCHC 3 cut(s) 134, 231, 1018
SfaNI GCATC 6 cut(s) 568, 601, 1036, 1168, 1324, 1474
SinI GGWCC 2 cut(s) 377, 877
SmiMI CAYNNNNRTG 1 cut(s) 1132
SmlI CTYRAG 1 cut(s) 1264
SmoI CTYRAG 1 cut(s) 1264
SnaBI TACGTA 1 cut(s) 425
SphI GCATGC 1 cut(s) 768
SsiI CCGC 5 cut(s) 168, 171, 177, 285, 870
SspMI CTAG 1 cut(s) 1523
SstI GAGCTC 2 cut(s) 134, 231
StyD4I CCNGG 6 cut(s) 203, 379, 890, 1009, 1185, 1494
StyI CCWWGG 3 cut(s) 620, 1238, 1410
TaaI ACNGT 3 cut(s) 256, 1115, 1134
TaiI ACGT 3 cut(s) 427, 601, 753
TaqI TCGA 6 cut(s) 264, 276, 556, 592, 1248, 1332
TatI WGTACW 2 cut(s) 160, 798
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 7 cut(s) 249, 407, 717, 738, 860, 1255, 1438
Tru1I TTAA 3 cut(s) 707, 846, 1265
Tru9I TTAA 3 cut(s) 707, 846, 1265
TscAI CASTG 4 cut(s) 259, 418, 748, 955
TseI GCWGC 6 cut(s) 111, 200, 614, 1190, 1364, 1367
TspDTI ATGAA 5 cut(s) 489, 1088, 1116, 1247, 1453
TspGWI ACGGA 3 cut(s) 107, 128, 262
TspRI CASTG 4 cut(s) 259, 418, 748, 955
Van91I CCANNNNNTGG 1 cut(s) 417
Vha464I CTTAAG 1 cut(s) 1264
VpaK11BI GGWCC 2 cut(s) 377, 877
XagI CCTNNNNNAGG 1 cut(s) 726
XapI RAATTY 4 cut(s) 440, 1172, 1271, 1460
XceI RCATGY 3 cut(s) 435, 768, 979
XmiI GTMKAC 1 cut(s) 1477
XmnI GAANNNNTTC 1 cut(s) 441
XspI CTAG 1 cut(s) 1523
ZrmI AGTACT 1 cut(s) 800
Zsp2I ATGCAT 1 cut(s) 1470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.