Rorug01G0258100

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0013141
Physical Location & Seq
Forward (+)
36766141 .. 36769095
2955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0258100.1

Sequence Viewer

Length: 2955 bp
ATGAAAACTTTGCTACATTTCTTCCTTTTCTTGATCACACTCCGAGTCAGTATCATCATCCCAGCAGTTCACAGCCAGTGTATTAAAGACCAGCAACAATTATTGCTCCAATTCAACAAAAGCCTCGTGTTCTTTGATTCTTTCCATCTTTCCACCAAGCTTATTTCTTGGAATGCAAGTACTGATTGTTGTTCTTGGGTCGGCGTCACTTGCAGCCGTAATGGGCGTGTTCTGGGTCTTGACTTAAGCAGCGAACATATCTTTGATGGAATTGACAATTCCAGCAGTCTCTTCCATCTTCATTATCTTCAAAGCCTCAATTTGGCCGACAACCAACTTGGCAGTATCTTTGAATCAATTCCATCCGCGATCGGAAATCTCCTGAACTTGAGGTATCTTAATTTATCTCTTAATTATTATTCAGGGCAAATTCCCATCGAGATTTCTCGCTTGAGGAGGTTGGAAGTTCTCGATTTTTCTAACTTCCATCATTTTGCTATCCCCTACAAAATTGGAAGTCCAAATTTATACATGTTGGTTCACAACCTCACAGAGCTTAGAGAGTTATATCTTGATGGTGTCCAAATATCAGAACAGGGGAGTGAGTGGTGCCGAGCCATATCTTCCCTTCCCAACCTCAGGGTCTTGAGCATGTCCTCTTGTAATCTTTCAGGCCCTTTTCTCGAGTCCCTTGCTAAGCTTCAGTCTCTATCAGTGATTAGGTTGGACGGGAACAATATCTCTGCGCCAGTTCCAGGATTCTTTGCCAACTTTTCAAACTTGACTGTCTTGAGTCTCCACAATTGCTCGTTGCATGGAACATTTCCCAAAGAGATCTTTCAGTTACCTTCTCTACAAAGCATTAACCTTTCAGATAATGACCAACTTGATGGTTCCTTGCCAGAATTCCCAAGGGATGGACCTCTTCAGCACCTGGATCTAAGCTGGACAAAATTTGCAGGGTTATTGCCAAATTCTATTGGCAACCTCAAAATGTTGTCCATCATAGATATTCATGATTGCAAGTTCACCGGACCGATCCCCAAGTCAATGATAAACCTTACACAATTGGTTTCTTTGTCGATGTCTCATAATAAGTTGGAAGGTTCAATTCCATCATTCAGTGGGGCCAATAATGTGGGCGACATAGACCTTTCCTACAATGGTCTAACAGGTACAATTAATTCCACCCACTGGAGAAACCTTACTAAGCTATCTCACCTCAGTTTGGGATCCAATAAGCTCGATGGGAATATCTCAGTATCTCTATTTTCTCTTCCTCTTTTGAAGTTGCAATGGCTAGATCTTTCCAGCAATCAATTCTTTGGTCCATTGCCTGAAATTTCAAATGTGTCTTCCTACTTGCTGAATGGCCTTGATCTTGAACTTGATTTGAGAAATCAATCTGACTTGCGCATTTTGGACCTTTCAGAGAACCAGATAAATGGCAAGATACCCGATTGGATTTGGAGTTTAAGTAATCTCATTCTCCTAAATCTTTCTTGCAACTCCCTAGATTCTCTAGAACTTCATTCATTTAAACTCACTAATCTTACTTACCTTGACCTTCATTCCAACCAGTTTCATGGGCAATTCCCAATGTTTCCATCACCTTGCAGTGCCTATTATCTAGATTTCTCCAGGAATTATTTCAACTCTATCATACCAACTACCATTGGAGATATGTTTCCCATCACAAATTTTCTCTCTCTTTCGAGCAATAACCTCAATGGGATCATTCCAGAATCATTGTGCAATTCACAATTTCTTGAGGTTCTTGATCTATCCAATAATTCTTTGAGTGGCAAGGTTCCCCAATGCTTAACTGCAATGACGAAGCTTGAAGTACTCAACATAAGGAGAAACAATCTTACAAATGTTGATGACTTCTCTCATAATTGCCAGTTAAAAGCTCTAGACATCAGTGAAAATCACCTTCAAGGCCGGTTTCTAAAATCTCTTGTCAACTGCACCCAGTTAGAGATCTTAAACCTTGGAAACAATTTGATAACAGGACCATTTCCATGCTTTTTAAGGAACACATCCACCTTGCGTGTCCTTGTCTTGCGATCCAATAAATTTTATGGGCGCATTGGATGTCACAAGACTAATGGCACTTGGCCAGTACTTCAAATCATAGACTTAGCTCACAACAATTTTAGTGGTGAAGTACTTGGAACAACTTTGACAACTTGGCAGGCGATGATGACTAGCAAAGATGATGCCCTACGGAAGATCAATGACCTTCAATATGAGTCGTTGGCTGGAGTTTATTATCAAGAAGATACAGTCTTGGTTACCAACAAAGGTATAGAGATGGAGCTGGTGAATGTTTTAACTATCTTCACCTCCATTGACTTCTCGTGCAACAAGTTCAACGGATCAATACCCAAGGAAATTGGAGAACTCAAATCATTGTATGCCCTCAACTTGTCCAACAATGCTTTCACAGGTGCAGTTCCAACATCACTGAGTAATTTGGGTCAGCTAGAGTCCTTGGACCTCTCGAAAAACAAACTGAGCGGTCATATCCCACCGGAGCTTACAAAACTCACTTTCCTTGCATTCTTGAATCTCTCGTATAATCAATTGGTCGGGAGGATACCAAGCGGTAATCAATTTTCAACATTTGAAGCAGCTTCCTTCCAGGGTAATAAAGGATTATGGGGGCCTCCTTTAACAGCAGATAATAACACAGGGTTCTCACCACCAAAGCAGGAAGCCAACCATTCAAATCCTGGACATGAGATTGATTGGGATATTATCTGTCCTGAAATTGGGTTTACATGTGGGTTTGGGATTGCCATCGGGTCACTTTTGTTTTGCAAGAGATGGAGGAAATGGTATTACAGTGCTATGTACAACATGCTTCTAAAGATATTCCCTCAGCTGGAACAAAGATTTGGTCATCACAGGAGACATGTTTACGTACATCAAAGATACTGGAGACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

984

Amino Acids

110.41

Weight (kDa)

6.37

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 72 6.2e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 76 - 290 1.8e-13 Leucine-rich repeat region
LRR_8 PF13855 236 - 294 4.6e-08 Leucine rich repeat
LRR_14 PF23598 309 - 532 1.9e-10 Leucine-rich repeat region
LRR_8 PF13855 471 - 528 1.8e-07 Leucine rich repeat
LRR_8 PF13855 569 - 624 7.5e-07 Leucine rich repeat
LRR_4 PF12799 590 - 631 3.7e-06 Leucine Rich repeats (2 copies)
LRR_8 PF13855 805 - 864 1.2e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61540
fragaria_vesca FvH4_7g15410 FvH4_7g15410 FvH4_7g15410
malus_domestica MD01G1071500.v1.1
prunus_persica Prupe.2G177700_v2.0.a1
pyrus_communis pycom01g10070
rosa_chinensis RchiOBHm_Chr1g0357731
rosa_laevigata RLG00000028002
rosa_multiflora Rmu_ssc0000399.1_g000023
rosa_roxburghii Rroxscaffold_4G00298570
rosa_rugosa Rorug01G0258000 Rorug01G0258100
rosa_samantha Rh1AG272000 Rh1BG239200 Rh1CG255900 Rh1DG267500
rosa_wichuraiana Rw1G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1415
AccB1I GGYRCC 1 cut(s) 609
AccB7I CCANNNNNTGG 2 cut(s) 917, 1194
AccBSI CCGCTC 1 cut(s) 2523
AccII CGCG 1 cut(s) 368
AciI CCGC 3 cut(s) 366, 2523, 2610
AclWI GGATC 7 cut(s) 945, 1033, 1227, 1240, 1742, 2064, 2389
AcoI YGGCCR 2 cut(s) 324, 2120
AcsI RAATTY 8 cut(s) 429, 523, 905, 953, 973, 1341, 1699, 2078
AcuI CTGAAG 2 cut(s) 686, 911
AcyI GRCGYC 1 cut(s) 204
AfaI GTAC 7 cut(s) 181, 1177, 1848, 2127, 2172, 2861, 2931
AfiI CCNNNNNNNGG 8 cut(s) 322, 639, 755, 917, 1194, 1228, 2777, 2890
AflII CTTAAG 1 cut(s) 244
AflIII ACRYGT 3 cut(s) 531, 2786, 2920
AjnI CCWGG 5 cut(s) 754, 933, 1640, 2646, 2737
AjuI GAANNNNNNNTTGG 4 cut(s) 2429, 2461, 2886, 2918
Alw26I GTCTC 6 cut(s) 293, 711, 800, 1092, 2911, 2941
AlwI GGATC 7 cut(s) 945, 1033, 1227, 1240, 1742, 2064, 2389
AlwNI CAGNNNCTG 1 cut(s) 934
Ama87I CYCGRG 1 cut(s) 683
AoxI GGCC 7 cut(s) 324, 673, 1128, 1372, 1942, 2120, 2669
ApeKI GCWGC 3 cut(s) 213, 249, 2636
ApoI RAATTY 8 cut(s) 429, 523, 905, 953, 973, 1341, 1699, 2078
ArsI GACNNNNNNTTYG 2 cut(s) 2890, 2922
AseI ATTAAT 1 cut(s) 1182
Asp700I GAANNNNTTC 2 cut(s) 357, 1649
AspLEI GCGC 3 cut(s) 748, 1416, 2091
AspS9I GGNCC 9 cut(s) 674, 920, 1034, 1128, 1328, 1423, 2015, 2500, 2669
AsuHPI GGTGA 8 cut(s) 1021, 1211, 1602, 1925, 2177, 2338, 2338, 2697
AvaI CYCGRG 1 cut(s) 683
AvaII GGWCC 6 cut(s) 920, 1034, 1328, 1423, 2015, 2500
AxyI CCTNAGG 1 cut(s) 638
BalI TGGCCA 1 cut(s) 2122
BamHI GGATCC 1 cut(s) 1232
BanI GGYRCC 1 cut(s) 609
BauI CACGAG 2 cut(s) 125, 2362
BbsI GAAGAC 1 cut(s) 1347
BbvCI CCTCAGC 1 cut(s) 2886
BbvI GCAGC 3 cut(s) 225, 261, 2648
BceAI ACGGC 1 cut(s) 201
BcgI CGANNNNNNTGC 2 cut(s) 674, 708
BciT130I CCWGG 5 cut(s) 756, 935, 1642, 2648, 2739
BciVI GTATCC 1 cut(s) 2595
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 6 cut(s) 293, 711, 800, 1092, 2911, 2941
BfaI CTAG 7 cut(s) 1301, 1514, 1523, 1631, 1916, 2211, 2489
BfrI CTTAAG 1 cut(s) 244
BfuI GTATCC 1 cut(s) 2595
BglII AGATCT 3 cut(s) 834, 1303, 1983
BisI GCNGC 3 cut(s) 214, 250, 2637
BlpI GCTNAGC 1 cut(s) 696
BlsI GCNGC 3 cut(s) 215, 251, 2638
BmcAI AGTACT 4 cut(s) 181, 1848, 2127, 2172
Bme1390I CCNGG 5 cut(s) 756, 935, 1642, 2648, 2739
Bme18I GGWCC 6 cut(s) 920, 1034, 1328, 1423, 2015, 2500
BmeT110I CYCGRG 1 cut(s) 683
BmgT120I GGNCC 9 cut(s) 674, 920, 1034, 1128, 1328, 1423, 2015, 2500, 2669
BmiI GGNNCC 6 cut(s) 611, 895, 1129, 1234, 1812, 2670
BmrFI CCNGG 5 cut(s) 756, 935, 1642, 2648, 2739
BmrI ACTGGG 1 cut(s) 1969
BmsI GCATC 1 cut(s) 2212
BmuI ACTGGG 1 cut(s) 1969
BpiI GAAGAC 1 cut(s) 1347
BpmI CTGGAG 3 cut(s) 1216, 1624, 2286
Bpu10I CCTNAGC 1 cut(s) 2886
Bpu1102I GCTNAGC 1 cut(s) 696
BpuEI CTTGAG 5 cut(s) 409, 472, 667, 811, 1790
BsaAI YACGTR 1 cut(s) 2929
BsaBI GATNNNNATC 1 cut(s) 2804
BsaHI GRCGYC 1 cut(s) 204
BsaJI CCNNGG 5 cut(s) 911, 1993, 2391, 2496, 2647
BsaWI WCCGGW 2 cut(s) 1031, 2536
BsaXI ACNNNNNCTCC 2 cut(s) 1462, 1492
Bsc4I CCNNNNNNNGG 8 cut(s) 322, 639, 755, 917, 1194, 1228, 2777, 2890
Bse118I RCCGGY 1 cut(s) 1944
Bse1I ACTGG 8 cut(s) 76, 749, 1199, 1579, 1903, 1975, 2123, 2948
Bse21I CCTNAGG 1 cut(s) 638
Bse3DI GCAATG 3 cut(s) 1301, 1331, 1836
Bse8I GATNNNNATC 1 cut(s) 2804
BseBI CCWGG 5 cut(s) 756, 935, 1642, 2648, 2739
BseDI CCNNGG 5 cut(s) 911, 1993, 2391, 2496, 2647
BseGI GGATG 5 cut(s) 57, 362, 922, 2042, 2102
BseJI GATNNNNATC 1 cut(s) 2804
BseLI CCNNNNNNNGG 8 cut(s) 322, 639, 755, 917, 1194, 1228, 2777, 2890
BseMI GCAATG 3 cut(s) 1301, 1331, 1836
BseMII CTCAG 6 cut(s) 652, 1237, 1272, 2462, 2510, 2900
BseNI ACTGG 8 cut(s) 76, 749, 1199, 1579, 1903, 1975, 2123, 2948
BseRI GAGGAG 1 cut(s) 469
BseXI GCAGC 3 cut(s) 225, 261, 2648
BseYI CCCAGC 1 cut(s) 61
BsgI GTGCAG 2 cut(s) 1954, 2475
Bsh1236I CGCG 1 cut(s) 368
Bsh1285I CGRYCG 1 cut(s) 372
BshFI GGCC 7 cut(s) 326, 675, 1130, 1374, 1944, 2122, 2671
BshNI GGYRCC 1 cut(s) 609
BsiEI CGRYCG 1 cut(s) 372
BsiHKCI CYCGRG 1 cut(s) 683
BsiSI CCGG 3 cut(s) 1032, 1945, 2537
BslFI GGGAC 1 cut(s) 673
BslI CCNNNNNNNGG 8 cut(s) 322, 639, 755, 917, 1194, 1228, 2777, 2890
BsmAI GTCTC 6 cut(s) 293, 711, 800, 1092, 2911, 2941
BsmBI CGTCTC 1 cut(s) 2941
BsmFI GGGAC 1 cut(s) 673
BsmI GAATGC 2 cut(s) 178, 2564
BsnI GGCC 7 cut(s) 326, 675, 1130, 1374, 1944, 2122, 2671
BsoBI CYCGRG 1 cut(s) 683
Bsp1407I TGTACA 1 cut(s) 2859
Bsp1720I GCTNAGC 1 cut(s) 696
BspACI CCGC 3 cut(s) 366, 2523, 2610
BspANI GGCC 7 cut(s) 326, 675, 1130, 1374, 1944, 2122, 2671
BspCNI CTCAG 6 cut(s) 651, 1236, 1271, 2463, 2511, 2899
BspFNI CGCG 1 cut(s) 368
BspHI TCATGA 1 cut(s) 1015
BspLI GGNNCC 6 cut(s) 611, 895, 1129, 1234, 1812, 2670
BspPI GGATC 7 cut(s) 945, 1033, 1227, 1240, 1742, 2064, 2389
BspT107I GGYRCC 1 cut(s) 609
BspTI CTTAAG 1 cut(s) 244
BsrBI CCGCTC 1 cut(s) 2523
BsrDI GCAATG 3 cut(s) 1301, 1331, 1836
BsrFI RCCGGY 1 cut(s) 1944
BsrGI TGTACA 1 cut(s) 2859
BsrI ACTGG 8 cut(s) 76, 749, 1199, 1579, 1903, 1975, 2123, 2948
BssAI RCCGGY 1 cut(s) 1944
BssECI CCNNGG 5 cut(s) 911, 1993, 2391, 2496, 2647
BssNI GRCGYC 1 cut(s) 204
BssSI CACGAG 2 cut(s) 125, 2362
BssT1I CCWWGG 4 cut(s) 911, 1993, 2391, 2496
Bst2BI CACGAG 2 cut(s) 125, 2362
Bst2UI CCWGG 5 cut(s) 756, 935, 1642, 2648, 2739
Bst4CI ACNGT 3 cut(s) 787, 2290, 2852
Bst6I CTCTTC 3 cut(s) 296, 930, 1281
BstACI GRCGYC 1 cut(s) 204
BstAFI CTTAAG 1 cut(s) 244
BstAUI TGTACA 1 cut(s) 2859
BstBAI YACGTR 1 cut(s) 2929
BstC8I GCNNGC 1 cut(s) 2199
BstEII GGTNACC 1 cut(s) 2296
BstF5I GGATG 5 cut(s) 57, 362, 922, 2042, 2102
BstFNI CGCG 1 cut(s) 368
BstHHI GCGC 3 cut(s) 748, 1416, 2091
BstMAI GTCTC 6 cut(s) 293, 711, 800, 1092, 2911, 2941
BstMCI CGRYCG 1 cut(s) 372
BstMWI GCNNNNNNNGC 1 cut(s) 210
BstNI CCWGG 5 cut(s) 756, 935, 1642, 2648, 2739
BstNSI RCATGY 5 cut(s) 535, 655, 2790, 2869, 2924
BstPI GGTNACC 1 cut(s) 2296
BstSCI CCNGG 5 cut(s) 754, 933, 1640, 2646, 2737
BstSNI TACGTA 1 cut(s) 2929
BstUI CGCG 1 cut(s) 368
BstV1I GCAGC 3 cut(s) 225, 261, 2648
BstV2I GAAGAC 1 cut(s) 1347
BstX2I RGATCY 5 cut(s) 834, 937, 1232, 1303, 1983
BstXI CCANNNNNNTGG 4 cut(s) 890, 1138, 1445, 1586
BstYI RGATCY 5 cut(s) 834, 937, 1232, 1303, 1983
Bsu36I CCTNAGG 1 cut(s) 638
BsuI GTATCC 1 cut(s) 2595
BsuRI GGCC 7 cut(s) 326, 675, 1130, 1374, 1944, 2122, 2671
BtgZI GCGATG 1 cut(s) 2216
BtsCI GGATG 5 cut(s) 57, 362, 922, 2042, 2102
BtsI GCAGTG 1 cut(s) 1624
BtsIMutI CAGTG 8 cut(s) 83, 720, 1129, 1192, 1624, 1930, 2468, 2857
Cac8I GCNNGC 1 cut(s) 2199
CaiI CAGNNNCTG 1 cut(s) 934
CciI TCATGA 1 cut(s) 1015
CfoI GCGC 3 cut(s) 748, 1416, 2091
Cfr10I RCCGGY 1 cut(s) 1944
Cfr13I GGNCC 9 cut(s) 674, 920, 1034, 1128, 1328, 1423, 2015, 2500, 2669
CpoI CGGWCCG 1 cut(s) 1034
CseI GACGC 1 cut(s) 193
Csp6I GTAC 7 cut(s) 180, 1176, 1847, 2126, 2171, 2860, 2930
CspCI CAANNNNNGTGG 2 cut(s) 2143, 2178
CspI CGGWCCG 1 cut(s) 1034
CviQI GTAC 7 cut(s) 180, 1176, 1847, 2126, 2171, 2860, 2930
DraI TTTAAA 1 cut(s) 1540
EaeI YGGCCR 2 cut(s) 324, 2120
Eam1104I CTCTTC 3 cut(s) 296, 930, 1281
EarI CTCTTC 3 cut(s) 296, 930, 1281
Eco105I TACGTA 1 cut(s) 2929
Eco130I CCWWGG 4 cut(s) 911, 1993, 2391, 2496
Eco47I GGWCC 6 cut(s) 920, 1034, 1328, 1423, 2015, 2500
Eco57I CTGAAG 2 cut(s) 686, 911
Eco81I CCTNAGG 1 cut(s) 638
Eco88I CYCGRG 1 cut(s) 683
Eco91I GGTNACC 1 cut(s) 2296
EcoO109I RGGNCCY 2 cut(s) 674, 2669
EcoO65I GGTNACC 1 cut(s) 2296
EcoRI GAATTC 1 cut(s) 905
EcoRII CCWGG 5 cut(s) 754, 933, 1640, 2646, 2737
EcoT14I CCWWGG 4 cut(s) 911, 1993, 2391, 2496
ErhI CCWWGG 4 cut(s) 911, 1993, 2391, 2496
Esp3I CGTCTC 1 cut(s) 2941
FaqI GGGAC 1 cut(s) 673
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 3 cut(s) 214, 250, 2637
FokI GGATG 5 cut(s) 44, 349, 929, 2029, 2109
Fsp4HI GCNGC 3 cut(s) 214, 250, 2637
FspBI CTAG 7 cut(s) 1301, 1514, 1523, 1631, 1916, 2211, 2489
FspI TGCGCA 1 cut(s) 1415
GlaI GCGC 3 cut(s) 747, 1415, 2090
GluI GCNGC 3 cut(s) 214, 250, 2637
GsaI CCCAGC 1 cut(s) 65
GsuI CTGGAG 3 cut(s) 1216, 1624, 2286
HaeIII GGCC 7 cut(s) 326, 675, 1130, 1374, 1944, 2122, 2671
HapII CCGG 3 cut(s) 1032, 1945, 2537
HgaI GACGC 1 cut(s) 193
HhaI GCGC 3 cut(s) 748, 1416, 2091
Hin1I GRCGYC 1 cut(s) 204
Hin6I GCGC 3 cut(s) 746, 1414, 2089
HinP1I GCGC 3 cut(s) 746, 1414, 2089
HincII GTYRAC 1 cut(s) 1966
HindII GTYRAC 1 cut(s) 1966
HindIII AAGCTT 3 cut(s) 158, 698, 1838
HpaII CCGG 3 cut(s) 1032, 1945, 2537
HphI GGTGA 8 cut(s) 1021, 1211, 1602, 1925, 2177, 2338, 2338, 2697
Hpy166II GTNNAC 6 cut(s) 70, 541, 1029, 1966, 2784, 2926
Hpy188I TCNGA 6 cut(s) 44, 374, 592, 874, 1408, 1432
Hpy8I GTNNAC 6 cut(s) 70, 541, 1029, 1966, 2784, 2926
HpyAV CCTTC 7 cut(s) 638, 858, 1097, 1577, 1946, 2255, 2653
HpyCH4III ACNGT 3 cut(s) 787, 2290, 2852
HpyCH4IV ACGT 2 cut(s) 2928, 2950
HpyF10VI GCNNNNNNNGC 1 cut(s) 210
HpySE526I ACGT 2 cut(s) 2928, 2950
Hsp92I GRCGYC 1 cut(s) 204
HspAI GCGC 3 cut(s) 746, 1414, 2089
Ksp22I TGATCA 1 cut(s) 33
LmnI GCTCC 3 cut(s) 111, 2320, 2539
Lsp1109I GCAGC 3 cut(s) 225, 261, 2648
LweI GCATC 1 cut(s) 2212
MaeI CTAG 7 cut(s) 1301, 1514, 1523, 1631, 1916, 2211, 2489
MaeII ACGT 2 cut(s) 2928, 2950
MaeIII GTNAC 5 cut(s) 205, 843, 2099, 2296, 2811
MbiI CCGCTC 1 cut(s) 2523
MfeI CAATTG 3 cut(s) 802, 1067, 2588
MflI RGATCY 5 cut(s) 834, 937, 1232, 1303, 1983
MlsI TGGCCA 1 cut(s) 2122
MluNI TGGCCA 1 cut(s) 2122
MlyI GAGTC 5 cut(s) 54, 695, 802, 2264, 2501
MmeI TCCRAC 6 cut(s) 441, 705, 1080, 1599, 2460, 2486
Mox20I TGGCCA 1 cut(s) 2122
MroXI GAANNNNTTC 2 cut(s) 357, 1649
MscI TGGCCA 1 cut(s) 2122
MslI CAYNNNNRTG 1 cut(s) 2023
Msp20I TGGCCA 1 cut(s) 2122
MspA1I CMGCKG 1 cut(s) 2890
MspCI CTTAAG 1 cut(s) 244
MspI CCGG 3 cut(s) 1032, 1945, 2537
MspR9I CCNGG 5 cut(s) 756, 935, 1642, 2648, 2739
MunI CAATTG 3 cut(s) 802, 1067, 2588
Mva1269I GAATGC 2 cut(s) 178, 2564
MvaI CCWGG 5 cut(s) 756, 935, 1642, 2648, 2739
MvnI CGCG 1 cut(s) 368
MwoI GCNNNNNNNGC 1 cut(s) 210
NlaIV GGNNCC 6 cut(s) 611, 895, 1129, 1234, 1812, 2670
NmeAIII GCCGAG 1 cut(s) 638
NmuCI GTSAC 3 cut(s) 205, 2099, 2811
NsbI TGCGCA 1 cut(s) 1415
NspI RCATGY 5 cut(s) 535, 655, 2790, 2869, 2924
PaeR7I CTCGAG 1 cut(s) 683
PagI TCATGA 1 cut(s) 1015
PciI ACATGT 3 cut(s) 531, 2786, 2920
PctI GAATGC 2 cut(s) 178, 2564
PdmI GAANNNNTTC 2 cut(s) 357, 1649
PfeI GAWTC 6 cut(s) 137, 353, 759, 1517, 1745, 2572
PflMI CCANNNNNTGG 2 cut(s) 917, 1194
PfoI TCCNGGA 3 cut(s) 754, 1640, 2737
PkrI GCNGC 3 cut(s) 215, 251, 2638
Ple19I CGATCG 1 cut(s) 372
PleI GAGTC 5 cut(s) 53, 694, 801, 2263, 2500
PpsI GAGTC 5 cut(s) 53, 694, 801, 2263, 2500
Ppu21I YACGTR 1 cut(s) 2929
PscI ACATGT 3 cut(s) 531, 2786, 2920
PshBI ATTAAT 1 cut(s) 1182
Psp6I CCWGG 5 cut(s) 754, 933, 1640, 2646, 2737
PspEI GGTNACC 1 cut(s) 2296
PspFI CCCAGC 1 cut(s) 61
PspGI CCWGG 5 cut(s) 754, 933, 1640, 2646, 2737
PspN4I GGNNCC 6 cut(s) 611, 895, 1129, 1234, 1812, 2670
PspPI GGNCC 9 cut(s) 674, 920, 1034, 1128, 1328, 1423, 2015, 2500, 2669
PstNI CAGNNNCTG 1 cut(s) 934
PsuI RGATCY 5 cut(s) 834, 937, 1232, 1303, 1983
PvuI CGATCG 1 cut(s) 372
PvuII CAGCTG 1 cut(s) 2890
RsaI GTAC 7 cut(s) 181, 1177, 1848, 2127, 2172, 2861, 2931
RsaNI GTAC 7 cut(s) 180, 1176, 1847, 2126, 2171, 2860, 2930
RseI CAYNNNNRTG 1 cut(s) 2023
Rsr2I CGGWCCG 1 cut(s) 1034
RsrII CGGWCCG 1 cut(s) 1034
SatI GCNGC 3 cut(s) 214, 250, 2637
Sau96I GGNCC 9 cut(s) 674, 920, 1034, 1128, 1328, 1423, 2015, 2500, 2669
ScaI AGTACT 4 cut(s) 181, 1848, 2127, 2172
SchI GAGTC 5 cut(s) 54, 695, 802, 2264, 2501
ScrFI CCNGG 5 cut(s) 756, 935, 1642, 2648, 2739
SfaNI GCATC 1 cut(s) 2212
Sfr274I CTCGAG 1 cut(s) 683
SinI GGWCC 6 cut(s) 920, 1034, 1328, 1423, 2015, 2500
SlaI CTCGAG 1 cut(s) 683
SmiMI CAYNNNNRTG 1 cut(s) 2023
SmlI CTYRAG 7 cut(s) 244, 388, 451, 646, 683, 790, 1769
SmoI CTYRAG 7 cut(s) 244, 388, 451, 646, 683, 790, 1769
SnaBI TACGTA 1 cut(s) 2929
SsiI CCGC 3 cut(s) 366, 2523, 2610
SspMI CTAG 7 cut(s) 1301, 1514, 1523, 1631, 1916, 2211, 2489
StyD4I CCNGG 5 cut(s) 754, 933, 1640, 2646, 2737
StyI CCWWGG 4 cut(s) 911, 1993, 2391, 2496
TaaI ACNGT 3 cut(s) 787, 2290, 2852
TaiI ACGT 2 cut(s) 2931, 2953
TaqI TCGA 7 cut(s) 438, 471, 684, 1082, 1245, 1715, 2507
TaqII GACCGA 1 cut(s) 1051
TatI WGTACW 5 cut(s) 179, 1846, 2125, 2170, 2859
TfiI GAWTC 6 cut(s) 137, 353, 759, 1517, 1745, 2572
TscAI CASTG 8 cut(s) 83, 720, 1129, 1199, 1624, 1930, 2475, 2857
TseFI GTSAC 3 cut(s) 205, 2099, 2811
TseI GCWGC 3 cut(s) 213, 249, 2636
Tsp45I GTSAC 3 cut(s) 205, 2099, 2811
TspDTI ATGAA 7 cut(s) 17, 290, 1004, 1520, 1524, 1559, 1574
TspGWI ACGGA 2 cut(s) 2245, 2394
TspRI CASTG 8 cut(s) 83, 720, 1129, 1199, 1624, 1930, 2475, 2857
Van91I CCANNNNNTGG 2 cut(s) 917, 1194
Vha464I CTTAAG 1 cut(s) 244
VpaK11BI GGWCC 6 cut(s) 920, 1034, 1328, 1423, 2015, 2500
VspI ATTAAT 1 cut(s) 1182
XapI RAATTY 8 cut(s) 429, 523, 905, 953, 973, 1341, 1699, 2078
XbaI TCTAGA 3 cut(s) 1522, 1630, 1915
XceI RCATGY 5 cut(s) 535, 655, 2790, 2869, 2924
XcmI CCANNNNNNNNNTGG 1 cut(s) 2735
XhoI CTCGAG 1 cut(s) 683
XmnI GAANNNNTTC 2 cut(s) 357, 1649
XspI CTAG 7 cut(s) 1301, 1514, 1523, 1631, 1916, 2211, 2489
ZrmI AGTACT 4 cut(s) 181, 1848, 2127, 2172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.