Rh1DG267500

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0013141
Physical Location & Seq
Forward (+)
48440562 .. 48444134
3573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG267500.1

Sequence Viewer

Length: 1230 bp
ATGTTGGCAACTCACGCGCAACCCGCTTTGTTAAACTCGCTCCTCCTCCGCATCTCTCTTCGCACACTTCCACTCATTCAAGTAACCAATCTTCGCTACTCGGCGCGTAGATTCACAGCCATGTCCGTACATACCGCCACCTCCGGCGATTCGCCGCAAGACCACGTCGCCGGGGACTGGTTTTCCGTACCGGAGCTCCGACTTCGCGACCACCGCTTCTTCGTGCCTCTAGACTACTCCGCCGATCCTAAGGCTTCGTCTAAGATTTCAATCTTCGCTCGCGAAGTTGTTTCGGTTGGCAAAGAAGAGCAACCACTGCCATACCTATTATTTCTACAAGGTGGACCTGGATTCGAAAGCCCGCGACCAACTGAAGCCAGTGGTTGGGTGCGTAAAGCCTGTGAAGAATATCGTGTCATATTGCTGGATCAGCGAGGAACAGGTTTGTCTACTCCTTTGACACCTTCATCAATGTTGCAACTGAAGTCTGATGTGAAGTTGGCTGATTACTTACAACATTTTCGAGCCAATAATATAGTAAATGATGCTGAGTTTATTCGAGTACGTTTAGTTCCTGATGCAGGACGTTGGACAATTTTGGGTCAGAGTTATGGTGGTTTTTGTGGGGTAACCTATTTGAGTTTTGCACCGCAAGGACTAAAACAAGTCCTATTAACTGGAGGTATCCCTCCAATAGGAAATGGATGCACTGCAGATATCGTGTACAGAGCATGCTTTGAACAGATTATACGTCAGAATGAGAAGTACTACAAAAGGTATCCTCAAGATACTGAGATTGTTCGTGAAGTTGTTAACTTTTTGTCAGAATCTGAGGGAGGCGGGGTGCCTCTTCCATCTGGGGGCATCCTAACCCCAAAGGGATTGCAAACACTTGGTCTTTCTGGCTTAGGATCCAGTACTGGTTTTGAGCGCTTACACTACATGTTTGAGAGGGTCTGGGATCCTGTACTAGTTCCAGGATCACCGAAGCAAATCAGTTATTACTTCTTGGATGCTTTTGAGAAATGGTCATCTTTTAATACAAATCCACTTTATGCTCTAATGCACGAGTCCATATACTGTCAGGGTGCTCCATCACGGTGGTCTGCTCACAGAGTAAGGGCTGAAGATGAGGGCAAATTTGATGCAGTCCAGGCTGCAAAAGAAGGCCGTCCTGTATATTTCACAGGGGAGGTAGTCATAGGCTCGAATATTATCTTTTCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

409

Amino Acids

45.51

Weight (kDa)

8.17

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 108 - 265 6.2e-12 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61540
fragaria_vesca FvH4_7g15410 FvH4_7g15410 FvH4_7g15410
malus_domestica MD01G1071500.v1.1
prunus_persica Prupe.2G177700_v2.0.a1
pyrus_communis pycom01g10070
rosa_chinensis RchiOBHm_Chr1g0357731
rosa_laevigata RLG00000028002
rosa_multiflora Rmu_ssc0000399.1_g000023
rosa_roxburghii Rroxscaffold_4G00298570
rosa_rugosa Rorug01G0258000 Rorug01G0258100
rosa_samantha Rh1AG272000 Rh1BG239200 Rh1CG255900 Rh1DG267500
rosa_wichuraiana Rw1G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 844
AccB7I CCANNNNNTGG 1 cut(s) 384
AccI GTMKAC 1 cut(s) 449
AccII CGCG 5 cut(s) 17, 106, 207, 282, 364
AciI CCGC 9 cut(s) 24, 49, 135, 155, 214, 240, 362, 650, 840
AclWI GGATC 7 cut(s) 239, 435, 906, 919, 956, 969, 988
AcsI RAATTY 1 cut(s) 1139
AcuI CTGAAG 3 cut(s) 393, 503, 1146
AfaI GTAC 7 cut(s) 129, 189, 564, 725, 767, 919, 969
AfeI AGCGCT 1 cut(s) 932
AfiI CCNNNNNNNGG 5 cut(s) 177, 384, 581, 695, 860
AflIII ACRYGT 1 cut(s) 942
AgsI TTSAA 4 cut(s) 80, 270, 740, 1224
AhlI ACTAGT 1 cut(s) 970
AjiI CACGTC 1 cut(s) 166
AjnI CCWGG 3 cut(s) 346, 976, 1152
AluBI AGCT 1 cut(s) 196
AluI AGCT 1 cut(s) 196
Alw21I GWGCWC 2 cut(s) 198, 1093
AlwI GGATC 7 cut(s) 239, 435, 906, 919, 956, 969, 988
AlwNI CAGNNNCTG 1 cut(s) 830
Aor51HI AGCGCT 1 cut(s) 932
AoxI GGCC 1 cut(s) 1168
ApeKI GCWGC 1 cut(s) 1157
ApoI RAATTY 1 cut(s) 1139
ArsI GACNNNNNNTTYG 2 cut(s) 880, 912
AspLEI GCGC 3 cut(s) 19, 106, 933
AspS9I GGNCC 1 cut(s) 344
AsuC2I CCSGG 1 cut(s) 172
AsuHPI GGTGA 1 cut(s) 975
AsuII TTCGAA 1 cut(s) 354
AvaII GGWCC 1 cut(s) 344
AxyI CCTNAGG 1 cut(s) 249
BamHI GGATCC 2 cut(s) 911, 961
BanI GGYRCC 1 cut(s) 844
BanII GRGCYC 1 cut(s) 198
BarI GAAGNNNNNNTAC 2 cut(s) 75, 107
BauI CACGAG 1 cut(s) 1067
Bbv12I GWGCWC 2 cut(s) 198, 1093
BbvI GCAGC 1 cut(s) 1144
BccI CCATC 2 cut(s) 862, 1102
BceAI ACGGC 1 cut(s) 1155
BciT130I CCWGG 3 cut(s) 348, 978, 1154
BciVI GTATCC 2 cut(s) 695, 789
BcnI CCSGG 1 cut(s) 172
BcuI ACTAGT 1 cut(s) 970
BfaI CTAG 2 cut(s) 230, 971
BfmI CTRYAG 1 cut(s) 711
BfoI RGCGCY 1 cut(s) 934
BfuI GTATCC 2 cut(s) 695, 789
BisI GCNGC 2 cut(s) 155, 1158
BlsI GCNGC 2 cut(s) 156, 1159
BmcAI AGTACT 2 cut(s) 767, 919
Bme1390I CCNGG 4 cut(s) 172, 348, 978, 1154
Bme18I GGWCC 1 cut(s) 344
BmgBI CACGTC 1 cut(s) 166
BmgT120I GGNCC 1 cut(s) 344
BmiI GGNNCC 3 cut(s) 846, 913, 963
BmrFI CCNGG 4 cut(s) 172, 348, 978, 1154
BmsI GCATC 7 cut(s) 60, 535, 568, 695, 873, 1003, 1135
BpmI CTGGAG 1 cut(s) 699
Bpu10I CCTNAGC 1 cut(s) 907
Bpu14I TTCGAA 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 768
BpuMI CCSGG 1 cut(s) 172
BsaBI GATNNNNATC 1 cut(s) 269
BsaJI CCNNGG 1 cut(s) 171
BsaWI WCCGGW 1 cut(s) 190
BsaXI ACNNNNNCTCC 4 cut(s) 180, 210, 828, 858
Bsc4I CCNNNNNNNGG 5 cut(s) 177, 384, 581, 695, 860
Bse1I ACTGG 5 cut(s) 182, 378, 682, 915, 925
Bse21I CCTNAGG 1 cut(s) 249
Bse8I GATNNNNATC 1 cut(s) 269
BseBI CCWGG 3 cut(s) 348, 978, 1154
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 3 cut(s) 710, 864, 1018
BseJI GATNNNNATC 1 cut(s) 269
BseLI CCNNNNNNNGG 5 cut(s) 177, 384, 581, 695, 860
BseMII CTCAG 3 cut(s) 540, 783, 822
BseNI ACTGG 5 cut(s) 182, 378, 682, 915, 925
BseRI GAGGAG 2 cut(s) 32, 35
BseXI GCAGC 1 cut(s) 1144
Bsh1236I CGCG 5 cut(s) 17, 106, 207, 282, 364
BshFI GGCC 1 cut(s) 1170
BshNI GGYRCC 1 cut(s) 844
BsiHKAI GWGCWC 2 cut(s) 198, 1093
BsiSI CCGG 3 cut(s) 144, 171, 191
BslFI GGGAC 1 cut(s) 188
BslI CCNNNNNNNGG 5 cut(s) 177, 384, 581, 695, 860
BsmFI GGGAC 1 cut(s) 188
BsnI GGCC 1 cut(s) 1170
Bsp119I TTCGAA 1 cut(s) 354
Bsp1286I GDGCHC 2 cut(s) 198, 1093
Bsp1407I TGTACA 1 cut(s) 723
Bsp143I GATC 5 cut(s) 244, 427, 911, 961, 980
Bsp68I TCGCGA 2 cut(s) 207, 282
BspACI CCGC 9 cut(s) 24, 49, 135, 155, 214, 240, 362, 650, 840
BspANI GGCC 1 cut(s) 1170
BspCNI CTCAG 3 cut(s) 541, 784, 823
BspFNI CGCG 5 cut(s) 17, 106, 207, 282, 364
BspLI GGNNCC 3 cut(s) 846, 913, 963
BspMAI CTGCAG 1 cut(s) 715
BspPI GGATC 7 cut(s) 239, 435, 906, 919, 956, 969, 988
BspQI GCTCTTC 1 cut(s) 300
BspT104I TTCGAA 1 cut(s) 354
BspT107I GGYRCC 1 cut(s) 844
BsrGI TGTACA 1 cut(s) 723
BsrI ACTGG 5 cut(s) 182, 378, 682, 915, 925
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 5 cut(s) 244, 427, 911, 961, 980
BssSI CACGAG 1 cut(s) 1067
Bst2BI CACGAG 1 cut(s) 1067
Bst2UI CCWGG 3 cut(s) 348, 978, 1154
Bst4CI ACNGT 2 cut(s) 1082, 1101
Bst6I CTCTTC 3 cut(s) 63, 300, 855
BstAPI GCANNNNNTGC 1 cut(s) 316
BstAUI TGTACA 1 cut(s) 723
BstBI TTCGAA 1 cut(s) 354
BstC8I GCNNGC 3 cut(s) 280, 362, 733
BstDEI CTNAG 6 cut(s) 249, 261, 549, 792, 831, 907
BstEII GGTNACC 1 cut(s) 628
BstENI CCTNNNNNAGG 2 cut(s) 579, 693
BstF5I GGATG 3 cut(s) 710, 864, 1018
BstFNI CGCG 5 cut(s) 17, 106, 207, 282, 364
BstH2I RGCGCY 1 cut(s) 934
BstHHI GCGC 3 cut(s) 19, 106, 933
BstKTI GATC 5 cut(s) 247, 430, 914, 964, 983
BstMBI GATC 5 cut(s) 244, 427, 911, 961, 980
BstMWI GCNNNNNNNGC 6 cut(s) 14, 23, 213, 316, 430, 1154
BstNI CCWGG 3 cut(s) 348, 978, 1154
BstNSI RCATGY 2 cut(s) 735, 946
BstPI GGTNACC 1 cut(s) 628
BstSCI CCNGG 4 cut(s) 170, 346, 976, 1152
BstSFI CTRYAG 1 cut(s) 711
BstUI CGCG 5 cut(s) 17, 106, 207, 282, 364
BstV1I GCAGC 1 cut(s) 1144
BstX2I RGATCY 2 cut(s) 911, 961
BstXI CCANNNNNNTGG 1 cut(s) 1101
BstYI RGATCY 2 cut(s) 911, 961
Bsu36I CCTNAGG 1 cut(s) 249
BsuI GTATCC 2 cut(s) 695, 789
BsuRI GGCC 1 cut(s) 1170
BtrI CACGTC 1 cut(s) 166
BtsCI GGATG 3 cut(s) 710, 864, 1018
BtsI GCAGTG 2 cut(s) 314, 708
BtsIMutI CAGTG 3 cut(s) 314, 385, 708
BtuMI TCGCGA 2 cut(s) 207, 282
Cac8I GCNNGC 3 cut(s) 280, 362, 733
CaiI CAGNNNCTG 1 cut(s) 830
CfoI GCGC 3 cut(s) 19, 106, 933
Cfr13I GGNCC 1 cut(s) 344
Csp6I GTAC 7 cut(s) 128, 188, 563, 724, 766, 918, 968
CviAII CATG 3 cut(s) 121, 732, 943
CviQI GTAC 7 cut(s) 128, 188, 563, 724, 766, 918, 968
DdeI CTNAG 6 cut(s) 249, 261, 549, 792, 831, 907
DpnI GATC 5 cut(s) 246, 429, 913, 963, 982
DpnII GATC 5 cut(s) 244, 427, 911, 961, 980
Eam1104I CTCTTC 3 cut(s) 63, 300, 855
EarI CTCTTC 3 cut(s) 63, 300, 855
EciI GGCGGA 1 cut(s) 229
Ecl136II GAGCTC 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 198
Eco32I GATATC 1 cut(s) 718
Eco47I GGWCC 1 cut(s) 344
Eco47III AGCGCT 1 cut(s) 932
Eco53kI GAGCTC 1 cut(s) 196
Eco57I CTGAAG 3 cut(s) 393, 503, 1146
Eco81I CCTNAGG 1 cut(s) 249
Eco91I GGTNACC 1 cut(s) 628
EcoICRI GAGCTC 1 cut(s) 196
EcoNI CCTNNNNNAGG 2 cut(s) 579, 693
EcoO65I GGTNACC 1 cut(s) 628
EcoRII CCWGG 3 cut(s) 346, 976, 1152
EcoRV GATATC 1 cut(s) 718
EcoT38I GRGCYC 1 cut(s) 198
FaeI CATG 3 cut(s) 124, 735, 946
FaqI GGGAC 1 cut(s) 188
FatI CATG 3 cut(s) 120, 731, 942
FauI CCCGC 3 cut(s) 31, 369, 833
FblI GTMKAC 1 cut(s) 449
Fnu4HI GCNGC 2 cut(s) 155, 1158
FokI GGATG 3 cut(s) 717, 851, 1025
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 2 cut(s) 155, 1158
FspBI CTAG 2 cut(s) 230, 971
GlaI GCGC 3 cut(s) 18, 105, 932
GluI GCNGC 2 cut(s) 155, 1158
GsuI CTGGAG 1 cut(s) 699
HaeII RGCGCY 1 cut(s) 934
HaeIII GGCC 1 cut(s) 1170
HapII CCGG 3 cut(s) 144, 171, 191
HhaI GCGC 3 cut(s) 19, 106, 933
Hin1II CATG 3 cut(s) 124, 735, 946
Hin6I GCGC 3 cut(s) 17, 104, 931
HinP1I GCGC 3 cut(s) 17, 104, 931
HincII GTYRAC 1 cut(s) 814
HindII GTYRAC 1 cut(s) 814
HinfI GANTC 5 cut(s) 111, 149, 351, 827, 1070
HpaI GTTAAC 1 cut(s) 814
HpaII CCGG 3 cut(s) 144, 171, 191
HphI GGTGA 1 cut(s) 975
Hpy166II GTNNAC 4 cut(s) 344, 450, 724, 814
Hpy188I TCNGA 6 cut(s) 200, 490, 606, 756, 826, 832
Hpy188III TCNNGA 6 cut(s) 206, 230, 281, 575, 785, 803
Hpy8I GTNNAC 4 cut(s) 344, 450, 724, 814
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 2 cut(s) 474, 1160
HpyCH4III ACNGT 2 cut(s) 1082, 1101
HpyCH4IV ACGT 4 cut(s) 165, 565, 586, 751
HpyCH4V TGCA 9 cut(s) 478, 581, 647, 708, 713, 886, 1066, 1148, 1160
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 23, 213, 316, 430, 1154
HpyF3I CTNAG 6 cut(s) 249, 261, 549, 792, 831, 907
HpySE526I ACGT 4 cut(s) 165, 565, 586, 751
Hsp92II CATG 3 cut(s) 124, 735, 946
HspAI GCGC 3 cut(s) 17, 104, 931
KspAI GTTAAC 1 cut(s) 814
Kzo9I GATC 5 cut(s) 244, 427, 911, 961, 980
LguI GCTCTTC 1 cut(s) 300
LmnI GCTCC 4 cut(s) 45, 193, 201, 1096
Lsp1109I GCAGC 1 cut(s) 1144
LweI GCATC 7 cut(s) 60, 535, 568, 695, 873, 1003, 1135
MaeI CTAG 2 cut(s) 230, 971
MaeII ACGT 4 cut(s) 165, 565, 586, 751
MaeIII GTNAC 2 cut(s) 82, 628
MalI GATC 5 cut(s) 246, 429, 913, 963, 982
MboI GATC 5 cut(s) 244, 427, 911, 961, 980
MboII GAAGA 8 cut(s) 50, 83, 211, 265, 317, 416, 842, 1139
MflI RGATCY 2 cut(s) 911, 961
MhlI GDGCHC 2 cut(s) 198, 1093
MluCI AATT 2 cut(s) 594, 1139
MlyI GAGTC 1 cut(s) 1079
MmeI TCCRAC 2 cut(s) 223, 569
MseI TTAA 4 cut(s) 32, 674, 813, 1038
MslI CAYNNNNRTG 2 cut(s) 119, 1099
MspI CCGG 3 cut(s) 144, 171, 191
MspR9I CCNGG 4 cut(s) 172, 348, 978, 1154
MvaI CCWGG 3 cut(s) 348, 978, 1154
MvnI CGCG 5 cut(s) 17, 106, 207, 282, 364
MwoI GCNNNNNNNGC 6 cut(s) 14, 23, 213, 316, 430, 1154
NciI CCSGG 1 cut(s) 172
NdeII GATC 5 cut(s) 244, 427, 911, 961, 980
NlaIII CATG 3 cut(s) 124, 735, 946
NlaIV GGNNCC 3 cut(s) 846, 913, 963
NmeAIII GCCGAG 1 cut(s) 80
NruI TCGCGA 2 cut(s) 207, 282
NspI RCATGY 2 cut(s) 735, 946
NspV TTCGAA 1 cut(s) 354
PaeI GCATGC 1 cut(s) 735
PciI ACATGT 1 cut(s) 942
PciSI GCTCTTC 1 cut(s) 300
PfeI GAWTC 4 cut(s) 111, 149, 351, 827
PflFI GACNNNGTC 1 cut(s) 164
PflMI CCANNNNNTGG 1 cut(s) 384
PfoI TCCNGGA 1 cut(s) 976
PkrI GCNGC 2 cut(s) 156, 1159
PleI GAGTC 1 cut(s) 1078
PpsI GAGTC 1 cut(s) 1078
PscI ACATGT 1 cut(s) 942
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 3 cut(s) 346, 976, 1152
PspEI GGTNACC 1 cut(s) 628
PspGI CCWGG 3 cut(s) 346, 976, 1152
PspN4I GGNNCC 3 cut(s) 846, 913, 963
PspPI GGNCC 1 cut(s) 344
PsrI GAACNNNNNNTAC 4 cut(s) 555, 587, 732, 764
PstI CTGCAG 1 cut(s) 715
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 2 cut(s) 911, 961
PsyI GACNNNGTC 1 cut(s) 164
RruI TCGCGA 2 cut(s) 207, 282
RsaI GTAC 7 cut(s) 129, 189, 564, 725, 767, 919, 969
RsaNI GTAC 7 cut(s) 128, 188, 563, 724, 766, 918, 968
RseI CAYNNNNRTG 2 cut(s) 119, 1099
SacI GAGCTC 1 cut(s) 198
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 4 cut(s) 32, 674, 813, 1038
SatI GCNGC 2 cut(s) 155, 1158
Sau3AI GATC 5 cut(s) 244, 427, 911, 961, 980
Sau96I GGNCC 1 cut(s) 344
ScaI AGTACT 2 cut(s) 767, 919
SchI GAGTC 1 cut(s) 1079
ScrFI CCNGG 4 cut(s) 172, 348, 978, 1154
SduI GDGCHC 2 cut(s) 198, 1093
SfaNI GCATC 7 cut(s) 60, 535, 568, 695, 873, 1003, 1135
SfcI CTRYAG 1 cut(s) 711
SfuI TTCGAA 1 cut(s) 354
SinI GGWCC 1 cut(s) 344
SmiMI CAYNNNNRTG 2 cut(s) 119, 1099
SmlI CTYRAG 1 cut(s) 783
SmoI CTYRAG 1 cut(s) 783
SpeI ACTAGT 1 cut(s) 970
SphI GCATGC 1 cut(s) 735
Sse9I AATT 2 cut(s) 594, 1139
SsiI CCGC 9 cut(s) 24, 49, 135, 155, 214, 240, 362, 650, 840
SspI AATATT 1 cut(s) 1213
SspMI CTAG 2 cut(s) 230, 971
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 4 cut(s) 170, 346, 976, 1152
TaaI ACNGT 2 cut(s) 1082, 1101
TaiI ACGT 4 cut(s) 168, 568, 589, 754
TaqI TCGA 4 cut(s) 354, 523, 559, 1208
TasI AATT 2 cut(s) 594, 1139
TatI WGTACW 4 cut(s) 723, 765, 917, 967
TauI GCSGC 1 cut(s) 157
TfiI GAWTC 4 cut(s) 111, 149, 351, 827
Tru1I TTAA 4 cut(s) 32, 674, 813, 1038
Tru9I TTAA 4 cut(s) 32, 674, 813, 1038
TscAI CASTG 3 cut(s) 321, 385, 715
TseI GCWGC 1 cut(s) 1157
TspDTI ATGAA 1 cut(s) 456
TspGWI ACGGA 2 cut(s) 115, 175
TspRI CASTG 3 cut(s) 321, 385, 715
Tth111I GACNNNGTC 1 cut(s) 164
Van91I CCANNNNNTGG 1 cut(s) 384
VpaK11BI GGWCC 1 cut(s) 344
XagI CCTNNNNNAGG 2 cut(s) 579, 693
XapI RAATTY 1 cut(s) 1139
XbaI TCTAGA 1 cut(s) 229
XceI RCATGY 2 cut(s) 735, 946
XmiI GTMKAC 1 cut(s) 449
XspI CTAG 2 cut(s) 230, 971
ZrmI AGTACT 2 cut(s) 767, 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.