RLG00000028002

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013141
Physical Location & Seq
Reverse (-)
17605052 .. 17609214
4163 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028002

Sequence Viewer

Length: 1257 bp
ATGTTGGCAACTCACGCGCAACCCGCTTTGATAAACTCGCTCCTCCTCCGCATTCCCTCTCTCTCTCTTCGCACGCTTCCAGCTATTCACGTAACCAATCTTCGCTACTTCAACCATCCACGGACCTCGGCGCGTAGATTCACGTCCATGTCCGTACCTACCGCCACCTCCGGCGACTCGCCGAAAGACCACGTCGCCGGGGACTGGTTTTCCGTACCGGAGCTCCGACTTCGCGACCACCGCTTCTTCGTGCCTCTAGACTACTCCGACGATCCTAAGGCTTCGTCTAAGATTTCAATCTTCGCTCGCGAGGTTGTTTCAGTTGGCAAAGAAGAGCAACCACTACCATACCTATTATTTCTACAAGGTGGACCTGGATTTGAAAGCCCGCGACCAACTGAAGCCAATGGTTGGGTGCGTAAAGCCTGTGAAGAATATCGTGTCATATTGCTGGATCAGCGAGGAACAGGTTTGTCTACTCCTTTGACACCTTCATCAATGTTGCAACTGAAGTCTGATGTGAAGTTGGCTGATTACTTACAACATTTTCGAGCCAATAATATAGTAAATGATGCCGAGTTTATTCGAGTACGTTTAGTTCCTGATGCAGGACGTTGGACAATTTTGGGTCAGAGTTATGGTGGTTTTTGTGGGGTAACCTATTTGAGTTTTGCACCGCAAGGACTAAAACAAGTCCTTTTAACTGGAGGTATCCCTCCAATAGGAAATGGATGCACTGCAGATATCGTGTACAGAGCATGCTTTGAGCAGATTATACGTCAGAATGAGAAGTACTACAAAAGGTATCCTCAAGATACTGAGATTGTTCGTGAAGTTGTTAACTTTTTGTCAGAATCTGAGGGAGGCGGGGTGCCTCTTCCATCTGGGGGCATCCTAACCCCAAAGGGATTGCAAACACTTGGTCTTTCTGGCTTAGGATCCAGTACTGGTTTTGAGCGCTTACACTACATGTTTGAGAGGGTCTGGGATCCTGTACTAGTTCCAGGATCACTGAAGCAAATCAGTTATTACTTCTTGAATGCTTTTGAGAAGTGGTCATCTTTTGATACAAATCCACTTTATGCTCTAATGCACGAGTCCATATACTGTCAGGGTGCTCCATCACAGTGGTCTGCTCACAGAGTAAGGGCTGAAGATGAGGGCAAATTTGATGCAGTCCAGGCTGCAAAAGAAGGCCGTCCTGTATATTTCACAGGGGAGGTAGTCATAGGCTCGAATATTCTCTTTTCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

419

Amino Acids

46.56

Weight (kDa)

8.17

Isoelectric Point (pI)

49.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_1 PF00561 117 - 274 4.6e-12 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61540
fragaria_vesca FvH4_7g15410 FvH4_7g15410 FvH4_7g15410
malus_domestica MD01G1071500.v1.1
prunus_persica Prupe.2G177700_v2.0.a1
pyrus_communis pycom01g10070
rosa_chinensis RchiOBHm_Chr1g0357731
rosa_laevigata RLG00000028002
rosa_multiflora Rmu_ssc0000399.1_g000023
rosa_roxburghii Rroxscaffold_4G00298570
rosa_rugosa Rorug01G0258000 Rorug01G0258100
rosa_samantha Rh1AG272000 Rh1BG239200 Rh1CG255900 Rh1DG267500
rosa_wichuraiana Rw1G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 871
AccB7I CCANNNNNTGG 1 cut(s) 411
AccI GTMKAC 1 cut(s) 476
AccII CGCG 5 cut(s) 17, 133, 234, 309, 391
AciI CCGC 7 cut(s) 24, 49, 162, 241, 389, 677, 867
AclWI GGATC 7 cut(s) 266, 462, 933, 946, 983, 996, 1015
AcsI RAATTY 1 cut(s) 1166
AcuI CTGAAG 4 cut(s) 420, 530, 1034, 1173
AfaI GTAC 7 cut(s) 156, 216, 591, 752, 794, 946, 996
AfeI AGCGCT 1 cut(s) 959
AfiI CCNNNNNNNGG 5 cut(s) 204, 411, 608, 722, 887
AflIII ACRYGT 1 cut(s) 969
AgsI TTSAA 5 cut(s) 112, 297, 383, 1039, 1251
AhlI ACTAGT 1 cut(s) 997
AjiI CACGTC 2 cut(s) 144, 193
AjnI CCWGG 3 cut(s) 373, 1003, 1179
AluBI AGCT 2 cut(s) 83, 223
AluI AGCT 2 cut(s) 83, 223
Alw21I GWGCWC 2 cut(s) 225, 1120
AlwI GGATC 7 cut(s) 266, 462, 933, 946, 983, 996, 1015
AlwNI CAGNNNCTG 1 cut(s) 857
Aor51HI AGCGCT 1 cut(s) 959
AoxI GGCC 1 cut(s) 1195
ApeKI GCWGC 1 cut(s) 1184
ApoI RAATTY 1 cut(s) 1166
ArsI GACNNNNNNTTYG 2 cut(s) 907, 939
AspLEI GCGC 3 cut(s) 19, 133, 960
AspS9I GGNCC 2 cut(s) 123, 371
AsuC2I CCSGG 1 cut(s) 199
AvaII GGWCC 2 cut(s) 123, 371
AxyI CCTNAGG 1 cut(s) 276
BamHI GGATCC 2 cut(s) 938, 988
BanI GGYRCC 1 cut(s) 871
BanII GRGCYC 1 cut(s) 225
BarI GAAGNNNNNNTAC 2 cut(s) 84, 116
BauI CACGAG 1 cut(s) 1094
Bbv12I GWGCWC 2 cut(s) 225, 1120
BbvI GCAGC 1 cut(s) 1171
BccI CCATC 3 cut(s) 123, 889, 1129
BceAI ACGGC 1 cut(s) 1182
BciT130I CCWGG 3 cut(s) 375, 1005, 1181
BciVI GTATCC 2 cut(s) 722, 816
BcnI CCSGG 1 cut(s) 199
BcuI ACTAGT 1 cut(s) 997
BfaI CTAG 2 cut(s) 257, 998
BfmI CTRYAG 1 cut(s) 738
BfoI RGCGCY 1 cut(s) 961
BfuI GTATCC 2 cut(s) 722, 816
BisI GCNGC 1 cut(s) 1185
BlsI GCNGC 1 cut(s) 1186
BmcAI AGTACT 2 cut(s) 794, 946
Bme1390I CCNGG 4 cut(s) 199, 375, 1005, 1181
Bme18I GGWCC 2 cut(s) 123, 371
BmgBI CACGTC 2 cut(s) 144, 193
BmgT120I GGNCC 2 cut(s) 123, 371
BmiI GGNNCC 3 cut(s) 873, 940, 990
BmrFI CCNGG 4 cut(s) 199, 375, 1005, 1181
BmsI GCATC 5 cut(s) 562, 595, 722, 900, 1162
BpmI CTGGAG 1 cut(s) 726
Bpu10I CCTNAGC 1 cut(s) 934
BpuEI CTTGAG 1 cut(s) 795
BpuMI CCSGG 1 cut(s) 199
BsaAI YACGTR 1 cut(s) 91
BsaBI GATNNNNATC 2 cut(s) 296, 1071
BsaJI CCNNGG 3 cut(s) 119, 126, 198
BsaWI WCCGGW 1 cut(s) 217
BsaXI ACNNNNNCTCC 4 cut(s) 207, 237, 855, 885
Bsc4I CCNNNNNNNGG 5 cut(s) 204, 411, 608, 722, 887
Bse1I ACTGG 4 cut(s) 209, 709, 942, 952
Bse21I CCTNAGG 1 cut(s) 276
Bse8I GATNNNNATC 2 cut(s) 296, 1071
BseBI CCWGG 3 cut(s) 375, 1005, 1181
BseDI CCNNGG 3 cut(s) 119, 126, 198
BseGI GGATG 3 cut(s) 115, 737, 891
BseJI GATNNNNATC 2 cut(s) 296, 1071
BseLI CCNNNNNNNGG 5 cut(s) 204, 411, 608, 722, 887
BseMII CTCAG 2 cut(s) 810, 849
BseNI ACTGG 4 cut(s) 209, 709, 942, 952
BseRI GAGGAG 2 cut(s) 32, 35
BseXI GCAGC 1 cut(s) 1171
Bsh1236I CGCG 5 cut(s) 17, 133, 234, 309, 391
BshFI GGCC 1 cut(s) 1197
BshNI GGYRCC 1 cut(s) 871
BsiHKAI GWGCWC 2 cut(s) 225, 1120
BsiSI CCGG 3 cut(s) 171, 198, 218
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 5 cut(s) 204, 411, 608, 722, 887
BsmFI GGGAC 1 cut(s) 215
BsmI GAATGC 2 cut(s) 51, 1045
BsnI GGCC 1 cut(s) 1197
Bsp1286I GDGCHC 2 cut(s) 225, 1120
Bsp1407I TGTACA 1 cut(s) 750
Bsp143I GATC 5 cut(s) 271, 454, 938, 988, 1007
Bsp68I TCGCGA 2 cut(s) 234, 309
BspACI CCGC 7 cut(s) 24, 49, 162, 241, 389, 677, 867
BspANI GGCC 1 cut(s) 1197
BspCNI CTCAG 2 cut(s) 811, 850
BspFNI CGCG 5 cut(s) 17, 133, 234, 309, 391
BspLI GGNNCC 3 cut(s) 873, 940, 990
BspMAI CTGCAG 1 cut(s) 742
BspPI GGATC 7 cut(s) 266, 462, 933, 946, 983, 996, 1015
BspQI GCTCTTC 1 cut(s) 327
BspT107I GGYRCC 1 cut(s) 871
BsrGI TGTACA 1 cut(s) 750
BsrI ACTGG 4 cut(s) 209, 709, 942, 952
BssECI CCNNGG 3 cut(s) 119, 126, 198
BssMI GATC 5 cut(s) 271, 454, 938, 988, 1007
BssSI CACGAG 1 cut(s) 1094
Bst2BI CACGAG 1 cut(s) 1094
Bst2UI CCWGG 3 cut(s) 375, 1005, 1181
Bst4CI ACNGT 2 cut(s) 1109, 1128
Bst6I CTCTTC 3 cut(s) 72, 327, 882
BstAUI TGTACA 1 cut(s) 750
BstBAI YACGTR 1 cut(s) 91
BstC8I GCNNGC 4 cut(s) 74, 307, 389, 760
BstDEI CTNAG 5 cut(s) 276, 288, 819, 858, 934
BstDSI CCRYGG 1 cut(s) 119
BstEII GGTNACC 1 cut(s) 655
BstENI CCTNNNNNAGG 2 cut(s) 606, 720
BstF5I GGATG 3 cut(s) 115, 737, 891
BstFNI CGCG 5 cut(s) 17, 133, 234, 309, 391
BstH2I RGCGCY 1 cut(s) 961
BstHHI GCGC 3 cut(s) 19, 133, 960
BstKTI GATC 5 cut(s) 274, 457, 941, 991, 1010
BstMBI GATC 5 cut(s) 271, 454, 938, 988, 1007
BstMWI GCNNNNNNNGC 5 cut(s) 14, 23, 240, 457, 1181
BstNI CCWGG 3 cut(s) 375, 1005, 1181
BstNSI RCATGY 2 cut(s) 762, 973
BstPI GGTNACC 1 cut(s) 655
BstSCI CCNGG 4 cut(s) 197, 373, 1003, 1179
BstSFI CTRYAG 1 cut(s) 738
BstUI CGCG 5 cut(s) 17, 133, 234, 309, 391
BstV1I GCAGC 1 cut(s) 1171
BstX2I RGATCY 2 cut(s) 938, 988
BstXI CCANNNNNNTGG 1 cut(s) 1128
BstYI RGATCY 2 cut(s) 938, 988
Bsu36I CCTNAGG 1 cut(s) 276
BsuI GTATCC 2 cut(s) 722, 816
BsuRI GGCC 1 cut(s) 1197
BtgI CCRYGG 1 cut(s) 119
BtrI CACGTC 2 cut(s) 144, 193
BtsCI GGATG 3 cut(s) 115, 737, 891
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 3 cut(s) 735, 1010, 1133
BtuMI TCGCGA 2 cut(s) 234, 309
Cac8I GCNNGC 4 cut(s) 74, 307, 389, 760
CaiI CAGNNNCTG 1 cut(s) 857
CfoI GCGC 3 cut(s) 19, 133, 960
Cfr13I GGNCC 2 cut(s) 123, 371
Csp6I GTAC 7 cut(s) 155, 215, 590, 751, 793, 945, 995
CviAII CATG 3 cut(s) 148, 759, 970
CviQI GTAC 7 cut(s) 155, 215, 590, 751, 793, 945, 995
DdeI CTNAG 5 cut(s) 276, 288, 819, 858, 934
DpnI GATC 5 cut(s) 273, 456, 940, 990, 1009
DpnII GATC 5 cut(s) 271, 454, 938, 988, 1007
Eam1104I CTCTTC 3 cut(s) 72, 327, 882
EarI CTCTTC 3 cut(s) 72, 327, 882
Ecl136II GAGCTC 1 cut(s) 223
Eco24I GRGCYC 1 cut(s) 225
Eco32I GATATC 1 cut(s) 745
Eco47I GGWCC 2 cut(s) 123, 371
Eco47III AGCGCT 1 cut(s) 959
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 4 cut(s) 420, 530, 1034, 1173
Eco81I CCTNAGG 1 cut(s) 276
Eco91I GGTNACC 1 cut(s) 655
EcoICRI GAGCTC 1 cut(s) 223
EcoNI CCTNNNNNAGG 2 cut(s) 606, 720
EcoO65I GGTNACC 1 cut(s) 655
EcoRII CCWGG 3 cut(s) 373, 1003, 1179
EcoRV GATATC 1 cut(s) 745
EcoT38I GRGCYC 1 cut(s) 225
FaeI CATG 3 cut(s) 151, 762, 973
FaqI GGGAC 1 cut(s) 215
FatI CATG 3 cut(s) 147, 758, 969
FauI CCCGC 3 cut(s) 31, 396, 860
FblI GTMKAC 1 cut(s) 476
Fnu4HI GCNGC 1 cut(s) 1185
FokI GGATG 3 cut(s) 102, 744, 878
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 1185
FspBI CTAG 2 cut(s) 257, 998
GlaI GCGC 3 cut(s) 18, 132, 959
GluI GCNGC 1 cut(s) 1185
GsuI CTGGAG 1 cut(s) 726
HaeII RGCGCY 1 cut(s) 961
HaeIII GGCC 1 cut(s) 1197
HapII CCGG 3 cut(s) 171, 198, 218
HhaI GCGC 3 cut(s) 19, 133, 960
Hin1II CATG 3 cut(s) 151, 762, 973
Hin6I GCGC 3 cut(s) 17, 131, 958
HinP1I GCGC 3 cut(s) 17, 131, 958
HincII GTYRAC 1 cut(s) 841
HindII GTYRAC 1 cut(s) 841
HinfI GANTC 4 cut(s) 138, 176, 854, 1097
HpaI GTTAAC 1 cut(s) 841
HpaII CCGG 3 cut(s) 171, 198, 218
Hpy166II GTNNAC 4 cut(s) 371, 477, 751, 841
Hpy188I TCNGA 7 cut(s) 227, 268, 517, 633, 783, 853, 859
Hpy188III TCNNGA 7 cut(s) 233, 257, 308, 602, 812, 830, 1036
Hpy8I GTNNAC 4 cut(s) 371, 477, 751, 841
Hpy99I CGWCG 2 cut(s) 197, 272
HpyAV CCTTC 2 cut(s) 501, 1187
HpyCH4III ACNGT 2 cut(s) 1109, 1128
HpyCH4IV ACGT 6 cut(s) 90, 143, 192, 592, 613, 778
HpyCH4V TGCA 9 cut(s) 505, 608, 674, 735, 740, 913, 1093, 1175, 1187
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 23, 240, 457, 1181
HpyF3I CTNAG 5 cut(s) 276, 288, 819, 858, 934
HpySE526I ACGT 6 cut(s) 90, 143, 192, 592, 613, 778
Hsp92II CATG 3 cut(s) 151, 762, 973
HspAI GCGC 3 cut(s) 17, 131, 958
KspAI GTTAAC 1 cut(s) 841
Kzo9I GATC 5 cut(s) 271, 454, 938, 988, 1007
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 4 cut(s) 45, 220, 228, 1123
Lsp1109I GCAGC 1 cut(s) 1171
LweI GCATC 5 cut(s) 562, 595, 722, 900, 1162
MaeI CTAG 2 cut(s) 257, 998
MaeII ACGT 6 cut(s) 90, 143, 192, 592, 613, 778
MaeIII GTNAC 2 cut(s) 91, 655
MalI GATC 5 cut(s) 273, 456, 940, 990, 1009
MboI GATC 5 cut(s) 271, 454, 938, 988, 1007
MboII GAAGA 8 cut(s) 59, 92, 238, 292, 344, 443, 869, 1166
MflI RGATCY 2 cut(s) 938, 988
MhlI GDGCHC 2 cut(s) 225, 1120
MluCI AATT 2 cut(s) 621, 1166
MlyI GAGTC 2 cut(s) 170, 1106
MmeI TCCRAC 3 cut(s) 250, 291, 596
MseI TTAA 2 cut(s) 701, 840
MslI CAYNNNNRTG 2 cut(s) 146, 1126
MspI CCGG 3 cut(s) 171, 198, 218
MspR9I CCNGG 4 cut(s) 199, 375, 1005, 1181
Mva1269I GAATGC 2 cut(s) 51, 1045
MvaI CCWGG 3 cut(s) 375, 1005, 1181
MvnI CGCG 5 cut(s) 17, 133, 234, 309, 391
MwoI GCNNNNNNNGC 5 cut(s) 14, 23, 240, 457, 1181
NciI CCSGG 1 cut(s) 199
NdeII GATC 5 cut(s) 271, 454, 938, 988, 1007
NlaIII CATG 3 cut(s) 151, 762, 973
NlaIV GGNNCC 3 cut(s) 873, 940, 990
NmeAIII GCCGAG 2 cut(s) 107, 601
NruI TCGCGA 2 cut(s) 234, 309
NspI RCATGY 2 cut(s) 762, 973
PaeI GCATGC 1 cut(s) 762
PciI ACATGT 1 cut(s) 969
PciSI GCTCTTC 1 cut(s) 327
PctI GAATGC 2 cut(s) 51, 1045
PfeI GAWTC 2 cut(s) 138, 854
PflFI GACNNNGTC 1 cut(s) 191
PflMI CCANNNNNTGG 1 cut(s) 411
PfoI TCCNGGA 1 cut(s) 1003
PkrI GCNGC 1 cut(s) 1186
PleI GAGTC 2 cut(s) 170, 1105
PpsI GAGTC 2 cut(s) 170, 1105
Ppu21I YACGTR 1 cut(s) 91
PscI ACATGT 1 cut(s) 969
Psp124BI GAGCTC 1 cut(s) 225
Psp6I CCWGG 3 cut(s) 373, 1003, 1179
PspEI GGTNACC 1 cut(s) 655
PspGI CCWGG 3 cut(s) 373, 1003, 1179
PspN4I GGNNCC 3 cut(s) 873, 940, 990
PspPI GGNCC 2 cut(s) 123, 371
PsrI GAACNNNNNNTAC 2 cut(s) 582, 614
PstI CTGCAG 1 cut(s) 742
PstNI CAGNNNCTG 1 cut(s) 857
PsuI RGATCY 2 cut(s) 938, 988
PsyI GACNNNGTC 1 cut(s) 191
RruI TCGCGA 2 cut(s) 234, 309
RsaI GTAC 7 cut(s) 156, 216, 591, 752, 794, 946, 996
RsaNI GTAC 7 cut(s) 155, 215, 590, 751, 793, 945, 995
RseI CAYNNNNRTG 2 cut(s) 146, 1126
SacI GAGCTC 1 cut(s) 225
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 2 cut(s) 701, 840
SatI GCNGC 1 cut(s) 1185
Sau3AI GATC 5 cut(s) 271, 454, 938, 988, 1007
Sau96I GGNCC 2 cut(s) 123, 371
ScaI AGTACT 2 cut(s) 794, 946
SchI GAGTC 2 cut(s) 170, 1106
ScrFI CCNGG 4 cut(s) 199, 375, 1005, 1181
SduI GDGCHC 2 cut(s) 225, 1120
SfaNI GCATC 5 cut(s) 562, 595, 722, 900, 1162
SfcI CTRYAG 1 cut(s) 738
SinI GGWCC 2 cut(s) 123, 371
SmiMI CAYNNNNRTG 2 cut(s) 146, 1126
SmlI CTYRAG 1 cut(s) 810
SmoI CTYRAG 1 cut(s) 810
SpeI ACTAGT 1 cut(s) 997
SphI GCATGC 1 cut(s) 762
Sse9I AATT 2 cut(s) 621, 1166
SsiI CCGC 7 cut(s) 24, 49, 162, 241, 389, 677, 867
SspI AATATT 1 cut(s) 1240
SspMI CTAG 2 cut(s) 257, 998
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 4 cut(s) 197, 373, 1003, 1179
TaaI ACNGT 2 cut(s) 1109, 1128
TaiI ACGT 6 cut(s) 93, 146, 195, 595, 616, 781
TaqI TCGA 3 cut(s) 550, 586, 1235
TasI AATT 2 cut(s) 621, 1166
TatI WGTACW 4 cut(s) 750, 792, 944, 994
TfiI GAWTC 2 cut(s) 138, 854
Tru1I TTAA 2 cut(s) 701, 840
Tru9I TTAA 2 cut(s) 701, 840
TscAI CASTG 3 cut(s) 742, 1017, 1133
TseI GCWGC 1 cut(s) 1184
TspDTI ATGAA 1 cut(s) 483
TspGWI ACGGA 3 cut(s) 136, 142, 202
TspRI CASTG 3 cut(s) 742, 1017, 1133
Tth111I GACNNNGTC 1 cut(s) 191
Van91I CCANNNNNTGG 1 cut(s) 411
VpaK11BI GGWCC 2 cut(s) 123, 371
XagI CCTNNNNNAGG 2 cut(s) 606, 720
XapI RAATTY 1 cut(s) 1166
XbaI TCTAGA 1 cut(s) 256
XceI RCATGY 2 cut(s) 762, 973
XmiI GTMKAC 1 cut(s) 476
XspI CTAG 2 cut(s) 257, 998
ZrmI AGTACT 2 cut(s) 794, 946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.