Prupe.2G177700_v2.0.a1

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0013141
Physical Location & Seq
Forward (+)
22254504 .. 22258954
4451 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G177700.1

Sequence Viewer

Length: 1551 bp
ATGCCGGCACTTCACGCGCCACCTTCATTGATAAGGTCACTCCTCTACGTTCCCTCTCCCTCCCTCTCTCTCAGTCTTCGCACACTTCCACTATTCCTCGTCACCAAGCTTCACTGCTTCAACTGCAGCCAGAGGTCAGTCCGTACAGTCACAGCCATGTCCTTGCCTAATGCCACCTCCGGCGAATCGTCGCCGGACCACGTTGCCGGGAAGTGGTTTTCGGTGCCGGAGCTCCGACTTCGTGACCATCGATTCACTGTGCCTCTCGACCACTCCGTCGGTCTTAAGGCTTCGTCTAAGATCTCAATCTTCGCTCGCGAAGTTGTTTCAGTTGGTAAAGAAGAACAACCACTGCCATACCTATTATATCTACAAGGTGGACCTGGATTTGAGGCCCCTCGACCAACCGAACCCAGTGGATGGATACGTAAAGCATGTGAAGAATTTCGTGTTATATTGATGGATCAGCGAGGAACAGGTTTATCAACTCCTTTGACAGCTTCATCTATGTCGCAACTGAAGTCTGAAGTGGATCTGGCTGATTACTTAAAACATTTCCGAGCTGATAATATAGTAAATGATGCAGAGTTTATTCGAGTACGTCTTGTGCCTGATGCTGGGCCTTGGACAATTTTGGGTCAGAGCTTTGGTGGTTTTTGTGCAGTAACGTATTTGAGTTTTGCACCACAAGGACTAAAACAAGTCCTTTTAACTGGAGGAATCCCTCCTATAGGAAATGGATGCACTGCAGATGCTGTGTATAAAGCGTGCTTTGAACAGATTATACATCAGAATGAGAAGTACTACCAAAGGTATCCTCAAGATATTGAGGTTGTTCGTGAAGTTGTTAACTATTTGTCAAAATCCGAGGGAGGAGGGGTCCAGCTTCCATCTGGAGGCTTCCTAACCCCAAAAGGATTGCAGATTCTTGGTCTTACTGGCTTGGGATCCAGTGCTGGTTTTGAGCGCTTACACTACATGTTTGAGAGGGCCTGGGATCCTATAATAGTTCCAGGAGCATCAAAGGAAATCAGTTATTACTTCTTGGATGCTTTTGACAAGTGGTCATCTTTTGACACAAATCCACTTTATGCTCTTCTTCATGAACCCATATACTGTCAGGGTGGTTCATCACGGTGGGCTGCTCAAAGAATAAGGGCGGAAAATGAGGGCAAATTTGATGCAGTCCGGGCTGCAAAAGAAGGTCGTCCCATATTTTTCACAGGAGAGATGATCTTTCCATGGATGTTTGATGAGATTCATGCCTTAAGGAAATTCAAAGGTGCTGCTCATATATTGGCTGAGAAGAAGGATTGGCCTCCACTATATGACATCACTGCACTAAATAATAACAAGGTACCTGTTGCAGCTGCTGTTTACTATGAAGACATGTTTGTAAACTTCAAGCTGGTCATGGAAACAGCATCTCAGATAGCCGGGATTCGGTTGTGGATAACTAACGAATTCATGCATTCTGGTCTGCGCGATGCAGGGAGCCAGGTTTTTGATCATTTGATGGGAATGTTAGACGGAAAGAAGCCTTTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_1

Protein Analysis

517

Amino Acids

57.44

Weight (kDa)

7.21

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013141)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G61540
fragaria_vesca FvH4_7g15410 FvH4_7g15410 FvH4_7g15410
malus_domestica MD01G1071500.v1.1
prunus_persica Prupe.2G177700_v2.0.a1
pyrus_communis pycom01g10070
rosa_chinensis RchiOBHm_Chr1g0357731
rosa_laevigata RLG00000028002
rosa_multiflora Rmu_ssc0000399.1_g000023
rosa_roxburghii Rroxscaffold_4G00298570
rosa_rugosa Rorug01G0258000 Rorug01G0258100
rosa_samantha Rh1AG272000 Rh1BG239200 Rh1CG255900 Rh1DG267500
rosa_wichuraiana Rw1G024270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 275
Acc65I GGTACC 1 cut(s) 1357
AccB1I GGYRCC 2 cut(s) 223, 1357
AccB7I CCANNNNNTGG 1 cut(s) 420
AccII CGCG 3 cut(s) 17, 318, 1485
AciI CCGC 1 cut(s) 1160
AclWI GGATC 6 cut(s) 471, 540, 942, 955, 992, 1005
AcsI RAATTY 4 cut(s) 443, 1175, 1274, 1463
AcuI CTGAAG 2 cut(s) 539, 546
AfaI GTAC 4 cut(s) 145, 600, 803, 1359
AfeI AGCGCT 1 cut(s) 968
AfiI CCNNNNNNNGG 6 cut(s) 213, 420, 617, 731, 896, 1443
AflII CTTAAG 2 cut(s) 284, 1267
AflIII ACRYGT 2 cut(s) 978, 1389
AgsI TTSAA 4 cut(s) 121, 776, 1279, 1405
AhdI GACNNNNNGTC 1 cut(s) 1063
AjnI CCWGG 4 cut(s) 382, 992, 1012, 1497
AluBI AGCT 8 cut(s) 109, 232, 500, 563, 645, 886, 1370, 1408
AluI AGCT 8 cut(s) 109, 232, 500, 563, 645, 886, 1370, 1408
Alw21I GWGCWC 1 cut(s) 234
AlwI GGATC 6 cut(s) 471, 540, 942, 955, 992, 1005
AlwNI CAGNNNCTG 2 cut(s) 755, 1373
Aor51HI AGCGCT 1 cut(s) 968
AoxI GGCC 4 cut(s) 393, 620, 990, 1316
ApeKI GCWGC 6 cut(s) 126, 1142, 1193, 1286, 1367, 1370
ApoI RAATTY 4 cut(s) 443, 1175, 1274, 1463
Asp700I GAANNNNTTC 1 cut(s) 444
Asp718I GGTACC 1 cut(s) 1357
AspLEI GCGC 3 cut(s) 19, 969, 1485
AspS9I GGNCC 6 cut(s) 196, 380, 394, 620, 880, 990
AsuC2I CCSGG 3 cut(s) 208, 1190, 1438
AsuHPI GGTGA 1 cut(s) 94
AvaII GGWCC 3 cut(s) 196, 380, 880
BamHI GGATCC 2 cut(s) 947, 997
BanI GGYRCC 2 cut(s) 223, 1357
BanII GRGCYC 1 cut(s) 234
BbsI GAAGAC 2 cut(s) 68, 1392
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 6 cut(s) 138, 1129, 1180, 1273, 1357, 1379
BccI CCATC 5 cut(s) 255, 414, 454, 898, 1510
BciT130I CCWGG 4 cut(s) 384, 994, 1014, 1499
BciVI GTATCC 2 cut(s) 417, 825
BclI TGATCA 1 cut(s) 1507
BcnI CCSGG 3 cut(s) 208, 1190, 1438
BfmI CTRYAG 3 cut(s) 124, 729, 747
BfoI RGCGCY 1 cut(s) 970
BfrI CTTAAG 2 cut(s) 284, 1267
BfuI GTATCC 2 cut(s) 417, 825
BglII AGATCT 1 cut(s) 300
BisI GCNGC 6 cut(s) 127, 1143, 1194, 1287, 1368, 1371
BlsI GCNGC 6 cut(s) 128, 1144, 1195, 1288, 1369, 1372
BmcAI AGTACT 1 cut(s) 803
Bme1390I CCNGG 7 cut(s) 208, 384, 994, 1014, 1190, 1438, 1499
Bme18I GGWCC 3 cut(s) 196, 380, 880
BmeRI GACNNNNNGTC 1 cut(s) 1063
BmgT120I GGNCC 6 cut(s) 196, 380, 394, 620, 880, 990
BmiI GGNNCC 7 cut(s) 225, 396, 881, 949, 999, 1359, 1496
BmrFI CCNGG 7 cut(s) 208, 384, 994, 1014, 1190, 1438, 1499
BmrI ACTGGG 1 cut(s) 408
BmsI GCATC 9 cut(s) 571, 604, 731, 742, 1028, 1039, 1171, 1433, 1477
BmuI ACTGGG 1 cut(s) 408
BpiI GAAGAC 2 cut(s) 68, 1392
BpmI CTGGAG 2 cut(s) 735, 915
BpuEI CTTGAG 1 cut(s) 804
BpuMI CCSGG 3 cut(s) 208, 1190, 1438
Bsa29I ATCGAT 1 cut(s) 250
BsaAI YACGTR 1 cut(s) 428
BsaBI GATNNNNATC 1 cut(s) 305
BsaJI CCNNGG 4 cut(s) 623, 867, 993, 1241
BsaXI ACNNNNNCTCC 2 cut(s) 864, 894
Bsc4I CCNNNNNNNGG 6 cut(s) 213, 420, 617, 731, 896, 1443
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 4 cut(s) 414, 718, 943, 951
Bse8I GATNNNNATC 1 cut(s) 305
BseBI CCWGG 4 cut(s) 384, 994, 1014, 1499
BseCI ATCGAT 1 cut(s) 250
BseDI CCNNGG 4 cut(s) 623, 867, 993, 1241
BseGI GGATG 4 cut(s) 425, 746, 1054, 1251
BseJI GATNNNNATC 1 cut(s) 305
BseLI CCNNNNNNNGG 6 cut(s) 213, 420, 617, 731, 896, 1443
BseMII CTCAG 3 cut(s) 85, 1293, 1442
BseNI ACTGG 4 cut(s) 414, 718, 943, 951
BseRI GAGGAG 2 cut(s) 32, 888
BseXI GCAGC 6 cut(s) 138, 1129, 1180, 1273, 1357, 1379
BseYI CCCAGC 1 cut(s) 617
BsgI GTGCAG 2 cut(s) 681, 1323
Bsh1236I CGCG 3 cut(s) 17, 318, 1485
BshFI GGCC 4 cut(s) 395, 622, 992, 1318
BshNI GGYRCC 2 cut(s) 223, 1357
BshVI ATCGAT 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 234
BsiSI CCGG 7 cut(s) 5, 180, 194, 207, 227, 1189, 1437
BslFI GGGAC 1 cut(s) 1194
BslI CCNNNNNNNGG 6 cut(s) 213, 420, 617, 731, 896, 1443
BsmFI GGGAC 1 cut(s) 1194
BsmI GAATGC 1 cut(s) 1471
BsnI GGCC 4 cut(s) 395, 622, 992, 1318
Bsp1286I GDGCHC 1 cut(s) 234
Bsp143I GATC 7 cut(s) 300, 463, 532, 947, 997, 1233, 1507
Bsp19I CCATGG 1 cut(s) 1241
Bsp68I TCGCGA 1 cut(s) 318
BspACI CCGC 1 cut(s) 1160
BspANI GGCC 4 cut(s) 395, 622, 992, 1318
BspCNI CTCAG 3 cut(s) 84, 1294, 1441
BspDI ATCGAT 1 cut(s) 250
BspFNI CGCG 3 cut(s) 17, 318, 1485
BspHI TCATGA 1 cut(s) 1102
BspLI GGNNCC 7 cut(s) 225, 396, 881, 949, 999, 1359, 1496
BspMAI CTGCAG 2 cut(s) 128, 751
BspPI GGATC 6 cut(s) 471, 540, 942, 955, 992, 1005
BspQI GCTCTTC 1 cut(s) 1101
BspT107I GGYRCC 2 cut(s) 223, 1357
BspTI CTTAAG 2 cut(s) 284, 1267
BsrFI RCCGGY 1 cut(s) 4
BsrI ACTGG 4 cut(s) 414, 718, 943, 951
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 4 cut(s) 623, 867, 993, 1241
BssMI GATC 7 cut(s) 300, 463, 532, 947, 997, 1233, 1507
BssT1I CCWWGG 2 cut(s) 623, 1241
Bst2UI CCWGG 4 cut(s) 384, 994, 1014, 1499
Bst4CI ACNGT 4 cut(s) 148, 259, 1118, 1137
Bst6I CTCTTC 1 cut(s) 1101
BstAFI CTTAAG 2 cut(s) 284, 1267
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 3 cut(s) 6, 316, 769
BstDEI CTNAG 4 cut(s) 71, 297, 1302, 1428
BstDSI CCRYGG 1 cut(s) 1241
BstENI CCTNNNNNAGG 1 cut(s) 729
BstF5I GGATG 4 cut(s) 425, 746, 1054, 1251
BstFNI CGCG 3 cut(s) 17, 318, 1485
BstH2I RGCGCY 1 cut(s) 970
BstHHI GCGC 3 cut(s) 19, 969, 1485
BstKTI GATC 7 cut(s) 303, 466, 535, 950, 1000, 1236, 1510
BstMBI GATC 7 cut(s) 300, 463, 532, 947, 997, 1233, 1507
BstMWI GCNNNNNNNGC 3 cut(s) 14, 123, 1190
BstNI CCWGG 4 cut(s) 384, 994, 1014, 1499
BstNSI RCATGY 3 cut(s) 438, 982, 1393
BstSCI CCNGG 7 cut(s) 206, 382, 992, 1012, 1188, 1436, 1497
BstSFI CTRYAG 3 cut(s) 124, 729, 747
BstSNI TACGTA 1 cut(s) 428
BstUI CGCG 3 cut(s) 17, 318, 1485
BstV1I GCAGC 6 cut(s) 138, 1129, 1180, 1273, 1357, 1379
BstV2I GAAGAC 2 cut(s) 68, 1392
BstX2I RGATCY 4 cut(s) 300, 532, 947, 997
BstYI RGATCY 4 cut(s) 300, 532, 947, 997
Bsu15I ATCGAT 1 cut(s) 250
BsuI GTATCC 2 cut(s) 417, 825
BsuRI GGCC 4 cut(s) 395, 622, 992, 1318
BsuTUI ATCGAT 1 cut(s) 250
BtgI CCRYGG 1 cut(s) 1241
BtgZI GCGATG 1 cut(s) 1500
BtsCI GGATG 4 cut(s) 425, 746, 1054, 1251
BtsI GCAGTG 4 cut(s) 112, 350, 744, 1335
BtsIMutI CAGTG 7 cut(s) 112, 255, 350, 421, 744, 958, 1335
BtuMI TCGCGA 1 cut(s) 318
Cac8I GCNNGC 3 cut(s) 6, 316, 769
CaiI CAGNNNCTG 2 cut(s) 755, 1373
CciI TCATGA 1 cut(s) 1102
CfoI GCGC 3 cut(s) 19, 969, 1485
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 6 cut(s) 196, 380, 394, 620, 880, 990
ClaI ATCGAT 1 cut(s) 250
Csp6I GTAC 4 cut(s) 144, 599, 802, 1358
CspCI CAANNNNNGTGG 2 cut(s) 9, 44
CviAII CATG 9 cut(s) 157, 435, 979, 1103, 1242, 1262, 1390, 1414, 1468
CviQI GTAC 4 cut(s) 144, 599, 802, 1358
DdeI CTNAG 4 cut(s) 71, 297, 1302, 1428
DpnI GATC 7 cut(s) 302, 465, 534, 949, 999, 1235, 1509
DpnII GATC 7 cut(s) 300, 463, 532, 947, 997, 1233, 1507
DrdI GACNNNNNNGTC 1 cut(s) 275
DriI GACNNNNNGTC 1 cut(s) 1063
DseDI GACNNNNNNGTC 1 cut(s) 275
Eam1104I CTCTTC 1 cut(s) 1101
Eam1105I GACNNNNNGTC 1 cut(s) 1063
EarI CTCTTC 1 cut(s) 1101
EciI GGCGGA 1 cut(s) 1175
Ecl136II GAGCTC 1 cut(s) 232
Eco105I TACGTA 1 cut(s) 428
Eco130I CCWWGG 2 cut(s) 623, 1241
Eco24I GRGCYC 1 cut(s) 234
Eco47I GGWCC 3 cut(s) 196, 380, 880
Eco47III AGCGCT 1 cut(s) 968
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 2 cut(s) 539, 546
EcoICRI GAGCTC 1 cut(s) 232
EcoNI CCTNNNNNAGG 1 cut(s) 729
EcoO109I RGGNCCY 2 cut(s) 394, 990
EcoRI GAATTC 1 cut(s) 1463
EcoRII CCWGG 4 cut(s) 382, 992, 1012, 1497
EcoT14I CCWWGG 2 cut(s) 623, 1241
EcoT22I ATGCAT 1 cut(s) 1473
EcoT38I GRGCYC 1 cut(s) 234
ErhI CCWWGG 2 cut(s) 623, 1241
FaeI CATG 9 cut(s) 160, 438, 982, 1106, 1245, 1265, 1393, 1417, 1471
FaqI GGGAC 1 cut(s) 1194
FatI CATG 9 cut(s) 156, 434, 978, 1102, 1241, 1261, 1389, 1413, 1467
FbaI TGATCA 1 cut(s) 1507
Fnu4HI GCNGC 6 cut(s) 127, 1143, 1194, 1287, 1368, 1371
FokI GGATG 4 cut(s) 432, 753, 1061, 1258
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 6 cut(s) 127, 1143, 1194, 1287, 1368, 1371
GlaI GCGC 3 cut(s) 18, 968, 1484
GluI GCNGC 6 cut(s) 127, 1143, 1194, 1287, 1368, 1371
GsaI CCCAGC 1 cut(s) 621
GsuI CTGGAG 2 cut(s) 735, 915
HaeII RGCGCY 1 cut(s) 970
HaeIII GGCC 4 cut(s) 395, 622, 992, 1318
HapII CCGG 7 cut(s) 5, 180, 194, 207, 227, 1189, 1437
HhaI GCGC 3 cut(s) 19, 969, 1485
Hin1II CATG 9 cut(s) 160, 438, 982, 1106, 1245, 1265, 1393, 1417, 1471
Hin6I GCGC 3 cut(s) 17, 967, 1483
HinP1I GCGC 3 cut(s) 17, 967, 1483
HincII GTYRAC 1 cut(s) 850
HindII GTYRAC 1 cut(s) 850
HindIII AAGCTT 1 cut(s) 107
HinfI GANTC 6 cut(s) 185, 252, 720, 925, 1258, 1441
HpaI GTTAAC 1 cut(s) 850
HpaII CCGG 7 cut(s) 5, 180, 194, 207, 227, 1189, 1437
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 4 cut(s) 380, 850, 1378, 1399
Hpy188I TCNGA 7 cut(s) 236, 526, 560, 642, 792, 868, 1431
Hpy188III TCNNGA 7 cut(s) 242, 266, 317, 821, 839, 894, 1103
Hpy8I GTNNAC 4 cut(s) 380, 850, 1378, 1399
Hpy99I CGWCG 2 cut(s) 193, 281
HpyAV CCTTC 3 cut(s) 33, 1196, 1303
HpyCH4III ACNGT 4 cut(s) 148, 259, 1118, 1137
HpyCH4IV ACGT 5 cut(s) 48, 201, 427, 601, 668
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 123, 1190
HpyF3I CTNAG 4 cut(s) 71, 297, 1302, 1428
HpySE526I ACGT 5 cut(s) 48, 201, 427, 601, 668
Hsp92II CATG 9 cut(s) 160, 438, 982, 1106, 1245, 1265, 1393, 1417, 1471
HspAI GCGC 3 cut(s) 17, 967, 1483
KpnI GGTACC 1 cut(s) 1361
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 1 cut(s) 1507
KspAI GTTAAC 1 cut(s) 850
Kzo9I GATC 7 cut(s) 300, 463, 532, 947, 997, 1233, 1507
LguI GCTCTTC 1 cut(s) 1101
LmnI GCTCC 4 cut(s) 229, 237, 1016, 1494
Lsp1109I GCAGC 6 cut(s) 138, 1129, 1180, 1273, 1357, 1379
LweI GCATC 9 cut(s) 571, 604, 731, 742, 1028, 1039, 1171, 1433, 1477
MaeII ACGT 5 cut(s) 48, 201, 427, 601, 668
MaeIII GTNAC 5 cut(s) 36, 100, 148, 242, 664
MalI GATC 7 cut(s) 302, 465, 534, 949, 999, 1235, 1509
MboI GATC 7 cut(s) 300, 463, 532, 947, 997, 1233, 1507
MboII GAAGA 8 cut(s) 68, 301, 353, 452, 1088, 1091, 1318, 1397
MflI RGATCY 4 cut(s) 300, 532, 947, 997
MhlI GDGCHC 1 cut(s) 234
MluCI AATT 5 cut(s) 443, 630, 1175, 1274, 1463
MmeI TCCRAC 1 cut(s) 259
Mph1103I ATGCAT 1 cut(s) 1473
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 1 cut(s) 444
MseI TTAA 5 cut(s) 285, 548, 710, 849, 1268
MslI CAYNNNNRTG 3 cut(s) 155, 792, 1135
MspA1I CMGCKG 1 cut(s) 1370
MspCI CTTAAG 2 cut(s) 284, 1267
MspI CCGG 7 cut(s) 5, 180, 194, 207, 227, 1189, 1437
MspR9I CCNGG 7 cut(s) 208, 384, 994, 1014, 1190, 1438, 1499
Mva1269I GAATGC 1 cut(s) 1471
MvaI CCWGG 4 cut(s) 384, 994, 1014, 1499
MvnI CGCG 3 cut(s) 17, 318, 1485
MwoI GCNNNNNNNGC 3 cut(s) 14, 123, 1190
NaeI GCCGGC 1 cut(s) 6
NciI CCSGG 3 cut(s) 208, 1190, 1438
NcoI CCATGG 1 cut(s) 1241
NdeII GATC 7 cut(s) 300, 463, 532, 947, 997, 1233, 1507
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 9 cut(s) 160, 438, 982, 1106, 1245, 1265, 1393, 1417, 1471
NlaIV GGNNCC 7 cut(s) 225, 396, 881, 949, 999, 1359, 1496
NmuCI GTSAC 4 cut(s) 36, 100, 148, 242
NruI TCGCGA 1 cut(s) 318
NsiI ATGCAT 1 cut(s) 1473
NspI RCATGY 3 cut(s) 438, 982, 1393
PagI TCATGA 1 cut(s) 1102
PciI ACATGT 2 cut(s) 978, 1389
PciSI GCTCTTC 1 cut(s) 1101
PcsI WCGNNNNNNNCGW 2 cut(s) 247, 273
PctI GAATGC 1 cut(s) 1471
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 6 cut(s) 185, 252, 720, 925, 1258, 1441
PflMI CCANNNNNTGG 1 cut(s) 420
PfoI TCCNGGA 1 cut(s) 1012
PkrI GCNGC 6 cut(s) 128, 1144, 1195, 1288, 1369, 1372
Ppu21I YACGTR 1 cut(s) 428
PscI ACATGT 2 cut(s) 978, 1389
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 4 cut(s) 382, 992, 1012, 1497
PspFI CCCAGC 1 cut(s) 617
PspGI CCWGG 4 cut(s) 382, 992, 1012, 1497
PspN4I GGNNCC 7 cut(s) 225, 396, 881, 949, 999, 1359, 1496
PspPI GGNCC 6 cut(s) 196, 380, 394, 620, 880, 990
PsrI GAACNNNNNNTAC 2 cut(s) 768, 800
PstI CTGCAG 2 cut(s) 128, 751
PstNI CAGNNNCTG 2 cut(s) 755, 1373
PsuI RGATCY 4 cut(s) 300, 532, 947, 997
PvuII CAGCTG 1 cut(s) 1370
RruI TCGCGA 1 cut(s) 318
RsaI GTAC 4 cut(s) 145, 600, 803, 1359
RsaNI GTAC 4 cut(s) 144, 599, 802, 1358
RseI CAYNNNNRTG 3 cut(s) 155, 792, 1135
SacI GAGCTC 1 cut(s) 234
SapI GCTCTTC 1 cut(s) 1101
SaqAI TTAA 5 cut(s) 285, 548, 710, 849, 1268
SatI GCNGC 6 cut(s) 127, 1143, 1194, 1287, 1368, 1371
Sau3AI GATC 7 cut(s) 300, 463, 532, 947, 997, 1233, 1507
Sau96I GGNCC 6 cut(s) 196, 380, 394, 620, 880, 990
ScaI AGTACT 1 cut(s) 803
ScrFI CCNGG 7 cut(s) 208, 384, 994, 1014, 1190, 1438, 1499
SduI GDGCHC 1 cut(s) 234
SfaNI GCATC 9 cut(s) 571, 604, 731, 742, 1028, 1039, 1171, 1433, 1477
SfcI CTRYAG 3 cut(s) 124, 729, 747
SinI GGWCC 3 cut(s) 196, 380, 880
SmiMI CAYNNNNRTG 3 cut(s) 155, 792, 1135
SmlI CTYRAG 3 cut(s) 284, 819, 1267
SmoI CTYRAG 3 cut(s) 284, 819, 1267
SnaBI TACGTA 1 cut(s) 428
Sse9I AATT 5 cut(s) 443, 630, 1175, 1274, 1463
SsiI CCGC 1 cut(s) 1160
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 7 cut(s) 206, 382, 992, 1012, 1188, 1436, 1497
StyI CCWWGG 2 cut(s) 623, 1241
TaaI ACNGT 4 cut(s) 148, 259, 1118, 1137
TaiI ACGT 5 cut(s) 51, 204, 430, 604, 671
TaqI TCGA 4 cut(s) 250, 267, 400, 595
TaqII GACCGA 1 cut(s) 269
TasI AATT 5 cut(s) 443, 630, 1175, 1274, 1463
TatI WGTACW 1 cut(s) 801
TfiI GAWTC 6 cut(s) 185, 252, 720, 925, 1258, 1441
Tru1I TTAA 5 cut(s) 285, 548, 710, 849, 1268
Tru9I TTAA 5 cut(s) 285, 548, 710, 849, 1268
TscAI CASTG 7 cut(s) 119, 262, 357, 421, 751, 958, 1342
TseFI GTSAC 4 cut(s) 36, 100, 148, 242
TseI GCWGC 6 cut(s) 126, 1142, 1193, 1286, 1367, 1370
Tsp45I GTSAC 4 cut(s) 36, 100, 148, 242
TspDTI ATGAA 8 cut(s) 15, 492, 1091, 1119, 1119, 1250, 1398, 1456
TspGWI ACGGA 3 cut(s) 131, 265, 1545
TspRI CASTG 7 cut(s) 119, 262, 357, 421, 751, 958, 1342
Van91I CCANNNNNTGG 1 cut(s) 420
Vha464I CTTAAG 2 cut(s) 284, 1267
VpaK11BI GGWCC 3 cut(s) 196, 380, 880
XagI CCTNNNNNAGG 1 cut(s) 729
XapI RAATTY 4 cut(s) 443, 1175, 1274, 1463
XceI RCATGY 3 cut(s) 438, 982, 1393
XcmI CCANNNNNNNNNTGG 1 cut(s) 890
XmnI GAANNNNTTC 1 cut(s) 444
ZrmI AGTACT 1 cut(s) 803
Zsp2I ATGCAT 1 cut(s) 1473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.