Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0014800

Physical Location & Seq

Genomic Coordinates Forward (+)

19181728 .. 19184862

Gene Structure 3135 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1085200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1968 bp

ATGAATTCAGGGGTCCGATTGGTATCGCCAGCAAGCAATGGGAAGTTGCCGGTGGTGAAGCTCCCTGATGAAGAGGCTGATTTTATAGAGAAGGACCCAAGCAATCGATATGTTCGGTACAATGAAATCTTGGGTAAGGGTGCCTTCAAGACTGTCTACAAGGCATTTGATGAAGTTGATGGAATAGAAGTGGCATGGAACCAAGTGAAGATTGATGCTGTCTTGCGATCGCCAGAAGATTTGGAGAAATTGTATTCTGAAGTCCATCTGCTGAAATCGGTGAAACATGACAATATTATTAAGTTCTATAATTCATGGGTGGATGATCAGAAAAATACGATTAACATGATTACGGAGCTCTTCACATCTGGAAGCCTAAGGCGATATCGTAAGAAGCATAAGAATGTTGATATGAAGGCCATAAGGAACTGGGCAAGGCAGATTCTCGGAGGTTTAGTCTTTCTCCACAGTCATAACCCCCCTATCATTCATAGAGACTTAAAATGTGATAATATTTTCATTAATGGAAATCTTGGAGAAGTTAAAATCGGAGACCTTGGATTGGCAACTGTTATGCAGCAGCCTACTGCTCGCAGTGTGATTGGGACTCCCGAATTTATGGCTCCAGAGCTCTATGAGGAGGAATATAATGAACTTGTTGATGTATACTCCTTCGGAATGTGCATGTTAGAGATGGTTACCTTTGAGTATCCATATTGTGAATGCAAAAGTCCTGCTCAAATATTTAAGAAGGTTACCTCTGGCGTTAAACCTGCTGCCCTTGGTAAGGTGAATGACCCCCAAATCAAAAGGTTTATTGAGAAATGTCTGGTTCCAGCATCTGAGAGGCTTTCGGCGAAAGAGCTGCTCAAAGATGCATTCCTTCAAGTTGACAATCCAAAGGAACCAATCCGCGATCCCTTCCAGTTACCTAACCAGAGTCTGAAAGCGACAAATTTACCAAAGTCCGGGCCTCTTTCCATGGACATAGATACTGACTACAAGCAGCTATCTATAAGCACATGTGCAGAAAGCAACAATGGGAGTCCACAGGTTCTGGAATTTCAAAGGGCACATAAGAACAACGAGTTCAGGTTAAGTGGGATAAAAAATGATGACGATTCAGTATCATTAACCTTGCGTATAGCTGATGCATGTGGTAAGGCAAGGAATATACATTTTCTCTTTTACATTGCTAGTGATACAGCAGTGTCTGTGGCGAGCGAGATGGTTGAACAACTGGAATTAACAAATCACGATGTGGCCTTCATTGCTGAGTTTATTGACTACTTGATAATGAAACTTCTACCCGGTTGGAAGCCCTCAATTGATTATTCCTCAAGTGGACCAACGAGTCTTTGTGGCAGATCCCCAGTCTATAGAAATGGAAAACCCTCGTTGTCAAGCCTGTGGGGTTCAGTGCTATCCGGTGCTCCTGCTGGTTCGATGGTAGAGCAAGATGGTAGCTCCTGTGATAAACCTGATACAATCAATTTTAACGGTGGCATTACCTCTTATCCAAGCTTTGCTGATTTTGCCGTTGAACATTCAAAAGTATCGGTTCACTCTGAGGTAATGGTTGATGATGGTTCCACCAAAAACGACCAAGCAGTTGAATCTGTCAATTGCAATACTTCTGAACTCGAGCTGAGGTATTCATATTTAGTTGACAGCAAGTTGCAGAGCGATTACTCTAGCAATGGAGGAGGCTTCCCAGTGAATGGAATCATAAAGAACTTGGAATTTCCATTGCATGAACATGATTTATCGAAAGATATAAGCTTGACAAGCAGCTGTTCTTCACTATCTTTGGCAGATAATGATCTGGATGCCGATTTAAAGTTGGAACTCGAAACGATTGAGGCACAGTACCAGCGGTGGTTCCAGGAACTTTCAAGGATGAGGGAGAAAGCATTGGAAACGACCAGAAAGAGATGGATGGATAAAAAGAAAGTGGCTGTTCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

OSR1_C Pkinase

Protein Analysis

656

Amino Acids

73.37

Weight (kDa)

5.49

Isoelectric Point (pI)

39.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	39 - 289	1.9e-35	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	41 - 294	3.4e-51	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014800)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g17050 FvH4_7g17050
malus_domestica	MD01G1085200.v1.1 MD07G1154200.v1.1
prunus_persica	Prupe.2G192800_v2.0.a1
pyrus_communis	pycom07g15170
rosa_chinensis	RchiOBHm_Chr1g0360461
rosa_laevigata	RLG00000027806
rosa_multiflora	Rmu_ssc0000337.1_g000001
rosa_roxburghii	Rroxscaffold_4G00296090
rosa_rugosa	Rorug01G0277900 Rorug01G0278000
rosa_samantha	Rh1AG291100 Rh1BG256100 Rh1CG273800 Rh1DG285900
rosa_wichuraiana	Rw1G025780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1353
Acc36I	ACCTGC	1 cut(s)	781
AccB1I	GGYRCC	1 cut(s)	140
AccB7I	CCANNNNNTGG	1 cut(s)	1721
AccI	GTMKAC	2 cut(s)	156, 666
AccII	CGCG	1 cut(s)	915
AciI	CCGC	2 cut(s)	913, 1876
AclWI	GGATC	2 cut(s)	911, 1362
AcsI	RAATTY	5 cut(s)	4, 614, 955, 1061, 1742
AcuI	CTGAAG	1 cut(s)	279
AdeI	CACNNNGTG	1 cut(s)	1261
AfaI	GTAC	2 cut(s)	119, 1871
AfiI	CCNNNNNNNGG	4 cut(s)	562, 787, 968, 1721
AflIII	ACRYGT	1 cut(s)	1022
AgsI	TTSAA	8 cut(s)	148, 887, 1067, 1235, 1544, 1551, 1616, 1896
AjnI	CCWGG	1 cut(s)	1884
Alw21I	GWGCWC	3 cut(s)	360, 633, 1435
Alw26I	GTCTC	2 cut(s)	489, 546
AlwI	GGATC	2 cut(s)	911, 1362
AlwNI	CAGNNNCTG	4 cut(s)	842, 943, 1057, 1214
Ama87I	CYCGRG	1 cut(s)	1643
AoxI	GGCC	3 cut(s)	417, 971, 1263
ApeKI	GCWGC	6 cut(s)	577, 580, 776, 865, 1006, 1791
ApoI	RAATTY	5 cut(s)	4, 614, 955, 1061, 1742
AseI	ATTAAT	1 cut(s)	522
AsiSI	GCGATCGC	1 cut(s)	230
AspS9I	GGNCC	4 cut(s)	13, 94, 971, 1346
AsuC2I	CCSGG	2 cut(s)	970, 1311
AsuHPI	GGTGA	3 cut(s)	67, 292, 802
AvaI	CYCGRG	1 cut(s)	1643
AvaII	GGWCC	3 cut(s)	13, 94, 1346
AxyI	CCTNAGG	1 cut(s)	377
BaeGI	GKGCMC	1 cut(s)	1075
BaeI	ACNNNNGTAYC	2 cut(s)	1194, 1227
BanI	GGYRCC	1 cut(s)	140
BanII	GRGCYC	2 cut(s)	360, 633
Bbv12I	GWGCWC	3 cut(s)	360, 633, 1435
BbvCI	CCTCAGC	1 cut(s)	1649
BbvI	GCAGC	6 cut(s)	589, 592, 763, 852, 1018, 1803
BccI	CCATC	9 cut(s)	173, 273, 688, 1222, 1441, 1454, 1580, 1929, 1933
BceAI	ACGGC	1 cut(s)	1523
BcgI	CGANNNNNNTGC	2 cut(s)	847, 881
BciT130I	CCWGG	1 cut(s)	1886
BciVI	GTATCC	1 cut(s)	720
BclI	TGATCA	1 cut(s)	325
BcnI	CCSGG	2 cut(s)	970, 1311
BcoDI	GTCTC	2 cut(s)	489, 546
BfaI	CTAG	2 cut(s)	1197, 1695
BfmI	CTRYAG	1 cut(s)	1378
BfuAI	ACCTGC	1 cut(s)	781
BfuI	GTATCC	1 cut(s)	720
BisI	GCNGC	6 cut(s)	578, 581, 777, 866, 1007, 1792
BlsI	GCNGC	6 cut(s)	579, 582, 778, 867, 1008, 1793
Bme1390I	CCNGG	3 cut(s)	970, 1311, 1886
Bme18I	GGWCC	3 cut(s)	13, 94, 1346
BmeT110I	CYCGRG	1 cut(s)	1643
BmgT120I	GGNCC	4 cut(s)	13, 94, 971, 1346
BmiI	GGNNCC	9 cut(s)	14, 96, 142, 200, 624, 834, 906, 1591, 1883
BmrFI	CCNGG	3 cut(s)	970, 1311, 1886
BmrI	ACTGGG	3 cut(s)	439, 1367, 1709
BmsI	GCATC	5 cut(s)	205, 848, 865, 1141, 1819
BmuI	ACTGGG	3 cut(s)	439, 1367, 1709
BpmI	CTGGAG	1 cut(s)	609
Bpu10I	CCTNAGC	1 cut(s)	1649
BpuEI	CTTGAG	1 cut(s)	1324
BpuMI	CCSGG	2 cut(s)	970, 1311
Bsa29I	ATCGAT	1 cut(s)	106
BsaBI	GATNNNNATC	2 cut(s)	22, 1821
BsaI	GGTCTC	1 cut(s)	546
BsaJI	CCNNGG	3 cut(s)	556, 781, 981
BsaWI	WCCGGW	1 cut(s)	1427
BsaXI	ACNNNNNCTCC	4 cut(s)	236, 266, 441, 471
Bsc4I	CCNNNNNNNGG	4 cut(s)	562, 787, 968, 1721
Bse118I	RCCGGY	1 cut(s)	49
Bse1I	ACTGG	5 cut(s)	434, 925, 1245, 1373, 1715
Bse21I	CCTNAGG	1 cut(s)	377
Bse3DI	GCAATG	5 cut(s)	43, 1191, 1269, 1705, 1748
Bse8I	GATNNNNATC	2 cut(s)	22, 1821
BseBI	CCWGG	1 cut(s)	1886
BseCI	ATCGAT	1 cut(s)	106
BseDI	CCNNGG	3 cut(s)	556, 781, 981
BseGI	GGATG	4 cut(s)	328, 1834, 1905, 1944
BseJI	GATNNNNATC	2 cut(s)	22, 1821
BseLI	CCNNNNNNNGG	4 cut(s)	562, 787, 968, 1721
BseMI	GCAATG	5 cut(s)	43, 1191, 1269, 1705, 1748
BseMII	CTCAG	4 cut(s)	834, 1266, 1560, 1640
BseNI	ACTGG	5 cut(s)	434, 925, 1245, 1373, 1715
BseRI	GAGGAG	2 cut(s)	653, 1719
BseSI	GKGCMC	1 cut(s)	1075
BseXI	GCAGC	6 cut(s)	589, 592, 763, 852, 1018, 1803
BsgI	GTGCAG	1 cut(s)	1047
Bsh1236I	CGCG	1 cut(s)	915
Bsh1285I	CGRYCG	1 cut(s)	230
BshFI	GGCC	3 cut(s)	419, 973, 1265
BshNI	GGYRCC	1 cut(s)	140
BshVI	ATCGAT	1 cut(s)	106
BsiEI	CGRYCG	1 cut(s)	230
BsiHKAI	GWGCWC	3 cut(s)	360, 633, 1435
BsiHKCI	CYCGRG	1 cut(s)	1643
BsiSI	CCGG	4 cut(s)	50, 969, 1311, 1428
BslFI	GGGAC	1 cut(s)	619
BslI	CCNNNNNNNGG	4 cut(s)	562, 787, 968, 1721
BsmAI	GTCTC	2 cut(s)	489, 546
BsmFI	GGGAC	1 cut(s)	619
BsmI	GAATGC	2 cut(s)	728, 878
BsnI	GGCC	3 cut(s)	419, 973, 1265
Bso31I	GGTCTC	1 cut(s)	546
BsoBI	CYCGRG	1 cut(s)	1643
Bsp1286I	GDGCHC	4 cut(s)	360, 633, 1075, 1435
Bsp143I	GATC	5 cut(s)	227, 325, 916, 1367, 1822
Bsp19I	CCATGG	1 cut(s)	981
BspACI	CCGC	2 cut(s)	913, 1876
BspANI	GGCC	3 cut(s)	419, 973, 1265
BspCNI	CTCAG	4 cut(s)	835, 1267, 1561, 1641
BspDI	ATCGAT	1 cut(s)	106
BspFNI	CGCG	1 cut(s)	915
BspLI	GGNNCC	9 cut(s)	14, 96, 142, 200, 624, 834, 906, 1591, 1883
BspMI	ACCTGC	1 cut(s)	781
BspPI	GGATC	2 cut(s)	911, 1362
BspQI	GCTCTTC	1 cut(s)	365
BspT107I	GGYRCC	1 cut(s)	140
BspTNI	GGTCTC	1 cut(s)	546
BsrDI	GCAATG	5 cut(s)	43, 1191, 1269, 1705, 1748
BsrFI	RCCGGY	1 cut(s)	49
BsrI	ACTGG	5 cut(s)	434, 925, 1245, 1373, 1715
BssAI	RCCGGY	1 cut(s)	49
BssECI	CCNNGG	3 cut(s)	556, 781, 981
BssMI	GATC	5 cut(s)	227, 325, 916, 1367, 1822
BssNAI	GTATAC	1 cut(s)	667
BssT1I	CCWWGG	3 cut(s)	556, 781, 981
Bst1107I	GTATAC	1 cut(s)	667
Bst2UI	CCWGG	1 cut(s)	1886
Bst4CI	ACNGT	5 cut(s)	154, 470, 571, 1502, 1869
Bst6I	CTCTTC	2 cut(s)	66, 365
BstC8I	GCNNGC	4 cut(s)	30, 34, 592, 1222
BstDEI	CTNAG	5 cut(s)	377, 843, 1275, 1569, 1649
BstDSI	CCRYGG	1 cut(s)	981
BstEII	GGTNACC	2 cut(s)	697, 754
BstENI	CCTNNNNNAGG	1 cut(s)	785
BstF5I	GGATG	4 cut(s)	328, 1834, 1905, 1944
BstFNI	CGCG	1 cut(s)	915
BstKTI	GATC	5 cut(s)	230, 328, 919, 1370, 1825
BstMAI	GTCTC	2 cut(s)	489, 546
BstMBI	GATC	5 cut(s)	227, 325, 916, 1367, 1822
BstMCI	CGRYCG	1 cut(s)	230
BstMWI	GCNNNNNNNGC	3 cut(s)	1271, 1535, 1788
BstNI	CCWGG	1 cut(s)	1886
BstNSI	RCATGY	3 cut(s)	688, 1026, 1158
BstPI	GGTNACC	2 cut(s)	697, 754
BstSCI	CCNGG	3 cut(s)	968, 1309, 1884
BstSFI	CTRYAG	1 cut(s)	1378
BstSLI	GKGCMC	1 cut(s)	1075
BstUI	CGCG	1 cut(s)	915
BstV1I	GCAGC	6 cut(s)	589, 592, 763, 852, 1018, 1803
BstX2I	RGATCY	1 cut(s)	1367
BstYI	RGATCY	1 cut(s)	1367
BstZ17I	GTATAC	1 cut(s)	667
Bsu15I	ATCGAT	1 cut(s)	106
Bsu36I	CCTNAGG	1 cut(s)	377
BsuI	GTATCC	1 cut(s)	720
BsuRI	GGCC	3 cut(s)	419, 973, 1265
BsuTUI	ATCGAT	1 cut(s)	106
BtgI	CCRYGG	1 cut(s)	981
BtsCI	GGATG	4 cut(s)	328, 1834, 1905, 1944
BtsI	GCAGTG	2 cut(s)	601, 1215
BtsIMutI	CAGTG	4 cut(s)	601, 1215, 1425, 1722
BveI	ACCTGC	1 cut(s)	781
Cac8I	GCNNGC	4 cut(s)	30, 34, 592, 1222
CaiI	CAGNNNCTG	4 cut(s)	842, 943, 1057, 1214
Cfr10I	RCCGGY	1 cut(s)	49
Cfr13I	GGNCC	4 cut(s)	13, 94, 971, 1346
ClaI	ATCGAT	1 cut(s)	106
Csp6I	GTAC	2 cut(s)	118, 1870
CviQI	GTAC	2 cut(s)	118, 1870
DdeI	CTNAG	5 cut(s)	377, 843, 1275, 1569, 1649
DpnI	GATC	5 cut(s)	229, 327, 918, 1369, 1824
DpnII	GATC	5 cut(s)	227, 325, 916, 1367, 1822
DraI	TTTAAA	1 cut(s)	1839
DraIII	CACNNNGTG	1 cut(s)	1261
DrdI	GACNNNNNNGTC	1 cut(s)	1353
DseDI	GACNNNNNNGTC	1 cut(s)	1353
Eam1104I	CTCTTC	2 cut(s)	66, 365
EarI	CTCTTC	2 cut(s)	66, 365
Ecl136II	GAGCTC	2 cut(s)	358, 631
Eco130I	CCWWGG	3 cut(s)	556, 781, 981
Eco24I	GRGCYC	2 cut(s)	360, 633
Eco31I	GGTCTC	1 cut(s)	546
Eco32I	GATATC	1 cut(s)	386
Eco47I	GGWCC	3 cut(s)	13, 94, 1346
Eco53kI	GAGCTC	2 cut(s)	358, 631
Eco57I	CTGAAG	1 cut(s)	279
Eco81I	CCTNAGG	1 cut(s)	377
Eco88I	CYCGRG	1 cut(s)	1643
Eco91I	GGTNACC	2 cut(s)	697, 754
EcoICRI	GAGCTC	2 cut(s)	358, 631
EcoNI	CCTNNNNNAGG	1 cut(s)	785
EcoO109I	RGGNCCY	1 cut(s)	94
EcoO65I	GGTNACC	2 cut(s)	697, 754
EcoRI	GAATTC	1 cut(s)	4
EcoRII	CCWGG	1 cut(s)	1884
EcoRV	GATATC	1 cut(s)	386
EcoT14I	CCWWGG	3 cut(s)	556, 781, 981
EcoT22I	ATGCAT	2 cut(s)	880, 1156
EcoT38I	GRGCYC	2 cut(s)	360, 633
ErhI	CCWWGG	3 cut(s)	556, 781, 981
FalI	AAGNNNNNCTT	2 cut(s)	128, 160
FaqI	GGGAC	1 cut(s)	619
FbaI	TGATCA	1 cut(s)	325
FblI	GTMKAC	2 cut(s)	156, 666
Fnu4HI	GCNGC	6 cut(s)	578, 581, 777, 866, 1007, 1792
FokI	GGATG	4 cut(s)	335, 1841, 1912, 1951
FriOI	GRGCYC	2 cut(s)	360, 633
Fsp4HI	GCNGC	6 cut(s)	578, 581, 777, 866, 1007, 1792
FspBI	CTAG	2 cut(s)	1197, 1695
GluI	GCNGC	6 cut(s)	578, 581, 777, 866, 1007, 1792
GsuI	CTGGAG	1 cut(s)	609
HaeIII	GGCC	3 cut(s)	419, 973, 1265
HapII	CCGG	4 cut(s)	50, 969, 1311, 1428
HincII	GTYRAC	2 cut(s)	892, 1669
HindII	GTYRAC	2 cut(s)	892, 1669
HindIII	AAGCTT	2 cut(s)	1522, 1780
HinfI	GANTC	8 cut(s)	442, 607, 940, 1045, 1121, 1354, 1616, 1725
HpaII	CCGG	4 cut(s)	50, 969, 1311, 1428
HphI	GGTGA	3 cut(s)	67, 292, 802
Hpy166II	GTNNAC	8 cut(s)	157, 667, 892, 1049, 1346, 1564, 1669, 1963
Hpy188III	TCNNGA	7 cut(s)	148, 369, 611, 626, 1058, 1256, 1826
Hpy8I	GTNNAC	8 cut(s)	157, 667, 892, 1049, 1346, 1564, 1669, 1963
HpyAV	CCTTC	8 cut(s)	85, 154, 409, 682, 745, 893, 931, 1276
HpyCH4III	ACNGT	5 cut(s)	154, 470, 571, 1502, 1869
HpyCH4V	TGCA	9 cut(s)	577, 684, 726, 878, 1028, 1154, 1629, 1681, 1753
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1271, 1535, 1788
HpyF3I	CTNAG	5 cut(s)	377, 843, 1275, 1569, 1649
Ksp22I	TGATCA	1 cut(s)	325
Kzo9I	GATC	5 cut(s)	227, 325, 916, 1367, 1822
LguI	GCTCTTC	1 cut(s)	365
LmnI	GCTCC	5 cut(s)	66, 355, 628, 1438, 1472
Lsp1109I	GCAGC	6 cut(s)	589, 592, 763, 852, 1018, 1803
LweI	GCATC	5 cut(s)	205, 848, 865, 1141, 1819
MaeI	CTAG	2 cut(s)	1197, 1695
MaeIII	GTNAC	3 cut(s)	697, 754, 927
MalI	GATC	5 cut(s)	229, 327, 918, 1369, 1824
MboI	GATC	5 cut(s)	227, 325, 916, 1367, 1822
MboII	GAAGA	5 cut(s)	83, 220, 248, 352, 1791
MfeI	CAATTG	2 cut(s)	1326, 1624
MflI	RGATCY	1 cut(s)	1367
MhlI	GDGCHC	4 cut(s)	360, 633, 1075, 1435
MlyI	GAGTC	4 cut(s)	601, 949, 1054, 1363
MmeI	TCCRAC	2 cut(s)	1295, 1824
Mph1103I	ATGCAT	2 cut(s)	880, 1156
MslI	CAYNNNNRTG	2 cut(s)	402, 1758
MspA1I	CMGCKG	2 cut(s)	1794, 1876
MspI	CCGG	4 cut(s)	50, 969, 1311, 1428
MspR9I	CCNGG	3 cut(s)	970, 1311, 1886
MunI	CAATTG	2 cut(s)	1326, 1624
Mva1269I	GAATGC	2 cut(s)	728, 878
MvaI	CCWGG	1 cut(s)	1886
MvnI	CGCG	1 cut(s)	915
MwoI	GCNNNNNNNGC	3 cut(s)	1271, 1535, 1788
NciI	CCSGG	2 cut(s)	970, 1311
NcoI	CCATGG	1 cut(s)	981
NdeII	GATC	5 cut(s)	227, 325, 916, 1367, 1822
NlaIV	GGNNCC	9 cut(s)	14, 96, 142, 200, 624, 834, 906, 1591, 1883
NsiI	ATGCAT	2 cut(s)	880, 1156
NspI	RCATGY	3 cut(s)	688, 1026, 1158
PaeR7I	CTCGAG	1 cut(s)	1643
PciI	ACATGT	1 cut(s)	1022
PciSI	GCTCTTC	1 cut(s)	365
PctI	GAATGC	2 cut(s)	728, 878
PfeI	GAWTC	4 cut(s)	442, 1121, 1616, 1725
PflMI	CCANNNNNTGG	1 cut(s)	1721
PfoI	TCCNGGA	1 cut(s)	1884
PkrI	GCNGC	6 cut(s)	579, 582, 778, 867, 1008, 1793
Ple19I	CGATCG	1 cut(s)	230
PleI	GAGTC	4 cut(s)	601, 948, 1053, 1362
PpsI	GAGTC	4 cut(s)	601, 948, 1053, 1362
PpuMI	RGGWCCY	1 cut(s)	94
PscI	ACATGT	1 cut(s)	1022
PshBI	ATTAAT	1 cut(s)	522
Psp124BI	GAGCTC	2 cut(s)	360, 633
Psp5II	RGGWCCY	1 cut(s)	94
Psp6I	CCWGG	1 cut(s)	1884
PspEI	GGTNACC	2 cut(s)	697, 754
PspGI	CCWGG	1 cut(s)	1884
PspN4I	GGNNCC	9 cut(s)	14, 96, 142, 200, 624, 834, 906, 1591, 1883
PspPI	GGNCC	4 cut(s)	13, 94, 971, 1346
PspPPI	RGGWCCY	1 cut(s)	94
PspXI	VCTCGAGB	1 cut(s)	1643
PstNI	CAGNNNCTG	4 cut(s)	842, 943, 1057, 1214
PsuI	RGATCY	1 cut(s)	1367
PvuI	CGATCG	1 cut(s)	230
PvuII	CAGCTG	1 cut(s)	1794
RgaI	GCGATCGC	1 cut(s)	230
RsaI	GTAC	2 cut(s)	119, 1871
RsaNI	GTAC	2 cut(s)	118, 1870
RseI	CAYNNNNRTG	2 cut(s)	402, 1758
SacI	GAGCTC	2 cut(s)	360, 633
SapI	GCTCTTC	1 cut(s)	365
SatI	GCNGC	6 cut(s)	578, 581, 777, 866, 1007, 1792
Sau3AI	GATC	5 cut(s)	227, 325, 916, 1367, 1822
Sau96I	GGNCC	4 cut(s)	13, 94, 971, 1346
SchI	GAGTC	4 cut(s)	601, 949, 1054, 1363
ScrFI	CCNGG	3 cut(s)	970, 1311, 1886
SduI	GDGCHC	4 cut(s)	360, 633, 1075, 1435
SfaAI	GCGATCGC	1 cut(s)	230
SfaNI	GCATC	5 cut(s)	205, 848, 865, 1141, 1819
SfcI	CTRYAG	1 cut(s)	1378
Sfr274I	CTCGAG	1 cut(s)	1643
SgfI	GCGATCGC	1 cut(s)	230
SinI	GGWCC	3 cut(s)	13, 94, 1346
SlaI	CTCGAG	1 cut(s)	1643
SmiMI	CAYNNNNRTG	2 cut(s)	402, 1758
SmlI	CTYRAG	2 cut(s)	1339, 1643
SmoI	CTYRAG	2 cut(s)	1339, 1643
SsiI	CCGC	2 cut(s)	913, 1876
SspI	AATATT	3 cut(s)	295, 514, 744
SspMI	CTAG	2 cut(s)	1197, 1695
SstI	GAGCTC	2 cut(s)	360, 633
StyD4I	CCNGG	3 cut(s)	968, 1309, 1884
StyI	CCWWGG	3 cut(s)	556, 781, 981
TaaI	ACNGT	5 cut(s)	154, 470, 571, 1502, 1869
TaqI	TCGA	5 cut(s)	106, 1445, 1644, 1769, 1851
TfiI	GAWTC	4 cut(s)	442, 1121, 1616, 1725
TscAI	CASTG	4 cut(s)	601, 1215, 1425, 1722
TseI	GCWGC	6 cut(s)	577, 580, 776, 865, 1006, 1791
TspGWI	ACGGA	1 cut(s)	368
TspRI	CASTG	4 cut(s)	601, 1215, 1425, 1722
Van91I	CCANNNNNTGG	1 cut(s)	1721
VpaK11BI	GGWCC	3 cut(s)	13, 94, 1346
VspI	ATTAAT	1 cut(s)	522
XagI	CCTNNNNNAGG	1 cut(s)	785
XapI	RAATTY	5 cut(s)	4, 614, 955, 1061, 1742
XceI	RCATGY	3 cut(s)	688, 1026, 1158
XhoI	CTCGAG	1 cut(s)	1643
XmiI	GTMKAC	2 cut(s)	156, 666
XspI	CTAG	2 cut(s)	1197, 1695
Zsp2I	ATGCAT	2 cut(s)	880, 1156

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.