Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0016604

Physical Location & Seq

Genomic Coordinates Forward (+)

20620390 .. 20622913

Gene Structure 2524 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1091100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2052 bp

ATGTTACCTCTATTTCTACCTTCCTTTCTCCTCCTTGGACTCCTTAATTCCTTACCATTTTCTCATTCCCTCCATGATCAGCAACAAAATCTAGAGCAACCCAATTCACTTCCCGACCAGCTTTGCCATGAAAAATGTGGACAACTTCAACTACCCTTCCCATTTCACTTGAACAAGTCTTGCAGTTCAGTCTCTGATGCTTTCCACCTCTCTTGCCAAAACTCAACAACCCTTTTCCTCAACATTGGTTCCGAAAGCTACCGCGTGCTAGAATTCTTCTCAGATGGTCTACTAGTGGACTTTCCGGGCTTCTCTTCGTGCAGCCAGTACAACGACTTGAACTCCTTCGATTTCTTGGGAAATGATCATTTAGGAGTCGCAGTTGAAAACGTGGTTGGCTTGTACGATTGTGAGGACTCTTCTTTGTGCAAGACAGATTGTGAAACAAATGACTTGCCTGGTTGTGATGGCAATGATAATGGCTCTCCTGCTTGCTGCTACCCCCTCTCTGATCATAGCGTGTGGCATCTCGGTGACAAGTTTTCGGTTTTTGCCAAGTTTGGATGCAGGGGGTTTTCCAGTTGGGTTGTGGAAAGAGGGTCTAACTTGGGGAAGAGAGGGATAAAATTGGAATGGGCAGTGCCAAAGAACTCATCCAAGGGAGTTTGTGATAGCAATGCTTATATTACTAATGCAACAGTGGTTGAAGGAGGGGTGAGATGCATGTGTCCAGATGGGTTCCTTGGTGATGGCTTTGCAGCTGGTGCAGGATGCTTGAAATGCCTCTATGTCAAAAGCCCTTTTATTTTTTCACATTGTTGGTGTTGCGCTGAAAACTTTAAAACAACCACTAGTATAACCATGGCATTACTTACAATTGACATGCCTTTGGCAGCATGCATCAAGGACAGAAAGGAAGCATATGGCAATGATTGCTTAAAGGAAAAGCATGGTGGCCGGAAACTTGTAATTTTCGGTGGAGTTCTAGCTCCAGTTTTCATCATAGCCTCCTCGATTGCACTTTTGTATATACTAAAGCGGCCTGCTAAGCCTGGGACATTTGATCCTGCTCAGAAAGTCCATTTTCACAACACAATATCATTCCGAAAAGCTAGTAGGATGCGGCTATTCACTTACCATGAGCTAGAGGAAGCTACGAAAGGGTTTGAGGAGGATCAGAAACTTGCACATGGCAACAATGGCACAATCTTTGCTGGGGTTCTTGGTGATGGATCGCACATTGCAGTGCACAAGGTGGAATGTGAAAAGGAAAAAGACCTAATTCAAGTCCTATCACAAATTGAGGTTTTATCTGCAGTTTTGCACCAAAATATTGCGCGACTCATTGGATGCTGCATTGACTTGGCCTACACTCCACTGCTGGTATATGAACATCCTGCGAATGGTACCCTCGAGGATCATTTGCATCAAAATGGAGGACAAAATGTTGCTCTTGACTGGTACAAGAGGCTGAACATTGCCGCTGAAACAGCAAGTGTTCTTGCCCTTTTGCAATACGAAATTTCTCCTCCCATTTTCCACTGTGATCTCAAATCTGCCTGCATCTTCATTGACGACAACTTTTCTAGCAAAGTTGCCGGATTTGCGCTACTGAATTCCAGTCCTGGAGATTGTTCTCAGTCACACAATCACGAAGGTTCGCACTTTCAGCAAAATGATGTTTATGCCTTTGGTATCATGCTATTTGAGATGATTGCAGGCTCAAATTGCTTGGACTTGCCGGCGGTAGCTCTGCAAAAGATAAGGAGTGGGAAGGTGGAAGAGATTGTGGATCCACTTCTCTACTATCACGAGCAACCTTCACACATGCGTGAGCAGATAGAGGTGGTTGCAGACCTTGCCATGAGGTGCTTGTTGTTTGGTGGAGATGGGAAGCTGGGAATGTATGACGTTGTGCATGAATTAGTACATATTAGAAGAGACAGCAGTGATGGAGGGAGCAAGAGAGGGCCTGGACTAGAGGAAACATTTTCAAACTCGAGCCTGCTTCAGATGATTTCGATGTCTCCTGATTCGAAATATGTGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase_Tyr

Protein Analysis

684

Amino Acids

75.05

Weight (kDa)

5.52

Isoelectric Point (pI)

46.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	42 - 92	3.1e-09	Wall-associated receptor kinase galacturonan-binding
Pkinase	PF00069	389 - 579	2.8e-12	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	391 - 575	1.7e-15	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016604)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46850
fragaria_vesca	FvH4_7g17870
malus_domestica	MD01G1091100.v1.1 MD07G1161300.v1.1
prunus_persica	Prupe.2G200600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0361321
rosa_laevigata	RLG00000027725
rosa_roxburghii	Rroxscaffold_4G00295040
rosa_rugosa	Rorug01G0288300
rosa_samantha	Rh1AG299500 Rh1BG263300 Rh1CG280900 Rh1DG293400
rosa_wichuraiana	Rw1G026510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1412
Acc65I	GGTACC	1 cut(s)	1406
AccB1I	GGYRCC	1 cut(s)	1406
AccI	GTMKAC	1 cut(s)	289
AccII	CGCG	2 cut(s)	264, 1339
AciI	CCGC	5 cut(s)	262, 1039, 1123, 1482, 1745
AclWI	GGATC	6 cut(s)	1058, 1182, 1240, 1425, 1787, 1800
AcoI	YGGCCR	1 cut(s)	955
AcsI	RAATTY	3 cut(s)	272, 1521, 1615
AcuI	CTGAAG	1 cut(s)	1994
AdeI	CACNNNGTG	1 cut(s)	1255
AfaI	GTAC	5 cut(s)	329, 404, 1408, 1463, 1929
AfiI	CCNNNNNNNGG	1 cut(s)	1403
AgsI	TTSAA	8 cut(s)	149, 172, 340, 386, 707, 778, 1286, 1995
AhlI	ACTAGT	2 cut(s)	292, 851
AjnI	CCWGG	4 cut(s)	457, 1051, 1624, 1972
AjuI	GAANNNNNNNTTGG	2 cut(s)	378, 410
AleI	CACNNNNGTG	1 cut(s)	1830
AluBI	AGCT	9 cut(s)	121, 258, 761, 989, 1112, 1144, 1154, 1751, 1897
AluI	AGCT	9 cut(s)	121, 258, 761, 989, 1112, 1144, 1154, 1751, 1897
Alw21I	GWGCWC	1 cut(s)	1251
Alw26I	GTCTC	3 cut(s)	196, 1935, 2031
Alw44I	GTGCAC	1 cut(s)	1247
AlwI	GGATC	6 cut(s)	1058, 1182, 1240, 1425, 1787, 1800
AlwNI	CAGNNNCTG	1 cut(s)	194
Ama87I	CYCGRG	2 cut(s)	1412, 1999
AoxI	GGCC	4 cut(s)	955, 1040, 1365, 1970
ApaLI	GTGCAC	1 cut(s)	1247
ApeKI	GCWGC	5 cut(s)	321, 495, 758, 893, 1353
ApoI	RAATTY	3 cut(s)	272, 1521, 1615
Asp700I	GAANNNNTTC	1 cut(s)	344
Asp718I	GGTACC	1 cut(s)	1406
AspLEI	GCGC	3 cut(s)	830, 1339, 1609
AspS9I	GGNCC	1 cut(s)	1970
AsuC2I	CCSGG	1 cut(s)	306
AsuHPI	GGTGA	4 cut(s)	545, 727, 758, 1238
AsuII	TTCGAA	1 cut(s)	2036
AvaI	CYCGRG	2 cut(s)	1412, 1999
BaeGI	GKGCMC	1 cut(s)	1251
BamHI	GGATCC	1 cut(s)	1792
BanI	GGYRCC	1 cut(s)	1406
BauI	CACGAG	1 cut(s)	1811
Bbv12I	GWGCWC	1 cut(s)	1251
BbvI	GCAGC	5 cut(s)	333, 482, 770, 905, 1340
BccI	CCATC	7 cut(s)	278, 461, 728, 743, 1223, 1883, 1946
BcgI	CGANNNNNNTGC	1 cut(s)	2026
BciT130I	CCWGG	4 cut(s)	459, 1053, 1626, 1974
BclI	TGATCA	3 cut(s)	76, 364, 511
BcnI	CCSGG	1 cut(s)	306
BcoDI	GTCTC	3 cut(s)	196, 1935, 2031
BcuI	ACTAGT	2 cut(s)	292, 851
BfaI	CTAG	9 cut(s)	92, 269, 293, 852, 986, 1113, 1145, 1587, 1979
BfmI	CTRYAG	1 cut(s)	1314
BisI	GCNGC	8 cut(s)	322, 496, 759, 894, 1040, 1124, 1354, 1482
BlpI	GCTNAGC	1 cut(s)	1047
BlsI	GCNGC	8 cut(s)	323, 497, 760, 895, 1041, 1125, 1355, 1483
Bme1390I	CCNGG	5 cut(s)	306, 459, 1053, 1626, 1974
BmeT110I	CYCGRG	2 cut(s)	1412, 1999
BmgT120I	GGNCC	1 cut(s)	1970
BmiI	GGNNCC	4 cut(s)	250, 740, 1408, 1794
BmrFI	CCNGG	5 cut(s)	306, 459, 1053, 1626, 1974
BpmI	CTGGAG	2 cut(s)	975, 1647
Bpu1102I	GCTNAGC	1 cut(s)	1047
Bpu14I	TTCGAA	1 cut(s)	2036
BpuMI	CCSGG	1 cut(s)	306
BsaJI	CCNNGG	5 cut(s)	34, 657, 742, 861, 1052
BsaXI	ACNNNNNCTCC	4 cut(s)	326, 356, 366, 396
Bsc4I	CCNNNNNNNGG	1 cut(s)	1403
Bse118I	RCCGGY	1 cut(s)	1741
Bse1I	ACTGG	5 cut(s)	325, 579, 992, 1463, 1620
Bse3DI	GCAATG	5 cut(s)	478, 682, 934, 1239, 1476
BseBI	CCWGG	4 cut(s)	459, 1053, 1626, 1974
BseDI	CCNNGG	5 cut(s)	34, 657, 742, 861, 1052
BseGI	GGATG	6 cut(s)	569, 653, 776, 1125, 1355, 1393
BseLI	CCNNNNNNNGG	1 cut(s)	1403
BseMI	GCAATG	5 cut(s)	478, 682, 934, 1239, 1476
BseMII	CTCAG	3 cut(s)	294, 1085, 1652
BseNI	ACTGG	5 cut(s)	325, 579, 992, 1463, 1620
BseRI	GAGGAG	4 cut(s)	20, 1000, 1184, 1518
BseSI	GKGCMC	1 cut(s)	1251
BseXI	GCAGC	5 cut(s)	333, 482, 770, 905, 1340
BseYI	CCCAGC	2 cut(s)	1214, 1897
BsgI	GTGCAG	2 cut(s)	340, 786
Bsh1236I	CGCG	2 cut(s)	264, 1339
BshFI	GGCC	4 cut(s)	957, 1042, 1367, 1972
BshNI	GGYRCC	1 cut(s)	1406
BsiHKAI	GWGCWC	1 cut(s)	1251
BsiHKCI	CYCGRG	2 cut(s)	1412, 1999
BsiSI	CCGG	4 cut(s)	305, 958, 1599, 1742
BslFI	GGGAC	1 cut(s)	1069
BslI	CCNNNNNNNGG	1 cut(s)	1403
BsmAI	GTCTC	3 cut(s)	196, 1935, 2031
BsmFI	GGGAC	1 cut(s)	1069
BsnI	GGCC	4 cut(s)	957, 1042, 1367, 1972
BsoBI	CYCGRG	2 cut(s)	1412, 1999
Bsp119I	TTCGAA	1 cut(s)	2036
Bsp1286I	GDGCHC	1 cut(s)	1251
Bsp143I	GATC	9 cut(s)	76, 364, 511, 1063, 1174, 1232, 1417, 1546, 1792
Bsp1720I	GCTNAGC	1 cut(s)	1047
Bsp19I	CCATGG	1 cut(s)	861
BspACI	CCGC	5 cut(s)	262, 1039, 1123, 1482, 1745
BspANI	GGCC	4 cut(s)	957, 1042, 1367, 1972
BspCNI	CTCAG	3 cut(s)	293, 1084, 1651
BspFNI	CGCG	2 cut(s)	264, 1339
BspLI	GGNNCC	4 cut(s)	250, 740, 1408, 1794
BspMAI	CTGCAG	1 cut(s)	1318
BspPI	GGATC	6 cut(s)	1058, 1182, 1240, 1425, 1787, 1800
BspT104I	TTCGAA	1 cut(s)	2036
BspT107I	GGYRCC	1 cut(s)	1406
BsrDI	GCAATG	5 cut(s)	478, 682, 934, 1239, 1476
BsrFI	RCCGGY	1 cut(s)	1741
BsrI	ACTGG	5 cut(s)	325, 579, 992, 1463, 1620
BssAI	RCCGGY	1 cut(s)	1741
BssECI	CCNNGG	5 cut(s)	34, 657, 742, 861, 1052
BssMI	GATC	9 cut(s)	76, 364, 511, 1063, 1174, 1232, 1417, 1546, 1792
BssSI	CACGAG	1 cut(s)	1811
BssT1I	CCWWGG	4 cut(s)	34, 657, 742, 861
Bst2BI	CACGAG	1 cut(s)	1811
Bst2UI	CCWGG	4 cut(s)	459, 1053, 1626, 1974
Bst4CI	ACNGT	2 cut(s)	700, 1544
Bst6I	CTCTTC	5 cut(s)	319, 424, 608, 1776, 1933
BstAPI	GCANNNNNTGC	3 cut(s)	764, 933, 1859
BstBI	TTCGAA	1 cut(s)	2036
BstC8I	GCNNGC	8 cut(s)	266, 493, 898, 1044, 1561, 1720, 1743, 2006
BstDEI	CTNAG	4 cut(s)	280, 1047, 1071, 1638
BstDSI	CCRYGG	1 cut(s)	861
BstF5I	GGATG	6 cut(s)	569, 653, 776, 1125, 1355, 1393
BstFNI	CGCG	2 cut(s)	264, 1339
BstHHI	GCGC	3 cut(s)	830, 1339, 1609
BstKTI	GATC	9 cut(s)	79, 367, 514, 1066, 1177, 1235, 1420, 1549, 1795
BstMAI	GTCTC	3 cut(s)	196, 1935, 2031
BstMBI	GATC	9 cut(s)	76, 364, 511, 1063, 1174, 1232, 1417, 1546, 1792
BstNI	CCWGG	4 cut(s)	459, 1053, 1626, 1974
BstNSI	RCATGY	4 cut(s)	727, 886, 900, 1831
BstSCI	CCNGG	5 cut(s)	304, 457, 1051, 1624, 1972
BstSFI	CTRYAG	1 cut(s)	1314
BstSLI	GKGCMC	1 cut(s)	1251
BstUI	CGCG	2 cut(s)	264, 1339
BstV1I	GCAGC	5 cut(s)	333, 482, 770, 905, 1340
BstX2I	RGATCY	1 cut(s)	1792
BstYI	RGATCY	1 cut(s)	1792
BsuRI	GGCC	4 cut(s)	957, 1042, 1367, 1972
BtgI	CCRYGG	1 cut(s)	861
BtsCI	GGATG	6 cut(s)	569, 653, 776, 1125, 1355, 1393
BtsI	GCAGTG	4 cut(s)	645, 1251, 1376, 1954
BtsIMutI	CAGTG	6 cut(s)	645, 705, 1251, 1376, 1540, 1954
Cac8I	GCNNGC	8 cut(s)	266, 493, 898, 1044, 1561, 1720, 1743, 2006
CaiI	CAGNNNCTG	1 cut(s)	194
CfoI	GCGC	3 cut(s)	830, 1339, 1609
Cfr10I	RCCGGY	1 cut(s)	1741
Cfr13I	GGNCC	1 cut(s)	1970
Csp6I	GTAC	5 cut(s)	328, 403, 1407, 1462, 1928
CspCI	CAANNNNNGTGG	2 cut(s)	194, 229
CviQI	GTAC	5 cut(s)	328, 403, 1407, 1462, 1928
DdeI	CTNAG	4 cut(s)	280, 1047, 1071, 1638
DpnI	GATC	9 cut(s)	78, 366, 513, 1065, 1176, 1234, 1419, 1548, 1794
DpnII	GATC	9 cut(s)	76, 364, 511, 1063, 1174, 1232, 1417, 1546, 1792
DraI	TTTAAA	1 cut(s)	841
DraIII	CACNNNGTG	1 cut(s)	1255
EaeI	YGGCCR	1 cut(s)	955
Eam1104I	CTCTTC	5 cut(s)	319, 424, 608, 1776, 1933
EarI	CTCTTC	5 cut(s)	319, 424, 608, 1776, 1933
Eco130I	CCWWGG	4 cut(s)	34, 657, 742, 861
Eco57I	CTGAAG	1 cut(s)	1994
Eco88I	CYCGRG	2 cut(s)	1412, 1999
EcoO109I	RGGNCCY	1 cut(s)	1970
EcoRI	GAATTC	2 cut(s)	272, 1615
EcoRII	CCWGG	4 cut(s)	457, 1051, 1624, 1972
EcoT14I	CCWWGG	4 cut(s)	34, 657, 742, 861
EcoT22I	ATGCAT	2 cut(s)	725, 902
ErhI	CCWWGG	4 cut(s)	34, 657, 742, 861
FaqI	GGGAC	1 cut(s)	1069
FauNDI	CATATG	1 cut(s)	922
FbaI	TGATCA	3 cut(s)	76, 364, 511
FblI	GTMKAC	1 cut(s)	289
Fnu4HI	GCNGC	8 cut(s)	322, 496, 759, 894, 1040, 1124, 1354, 1482
FokI	GGATG	6 cut(s)	576, 640, 783, 1132, 1362, 1380
Fsp4HI	GCNGC	8 cut(s)	322, 496, 759, 894, 1040, 1124, 1354, 1482
FspBI	CTAG	9 cut(s)	92, 269, 293, 852, 986, 1113, 1145, 1587, 1979
GlaI	GCGC	3 cut(s)	829, 1338, 1608
GluI	GCNGC	8 cut(s)	322, 496, 759, 894, 1040, 1124, 1354, 1482
GsaI	CCCAGC	2 cut(s)	1218, 1901
GsuI	CTGGAG	2 cut(s)	975, 1647
HaeIII	GGCC	4 cut(s)	957, 1042, 1367, 1972
HapII	CCGG	4 cut(s)	305, 958, 1599, 1742
HhaI	GCGC	3 cut(s)	830, 1339, 1609
Hin6I	GCGC	3 cut(s)	828, 1337, 1607
HinP1I	GCGC	3 cut(s)	828, 1337, 1607
HinfI	GANTC	5 cut(s)	39, 375, 416, 1341, 2033
HpaII	CCGG	4 cut(s)	305, 958, 1599, 1742
HphI	GGTGA	4 cut(s)	545, 727, 758, 1238
Hpy166II	GTNNAC	4 cut(s)	140, 290, 298, 1249
Hpy188I	TCNGA	8 cut(s)	196, 253, 283, 511, 1074, 1106, 1179, 2013
Hpy188III	TCNNGA	7 cut(s)	92, 113, 731, 1454, 1652, 1811, 2030
Hpy8I	GTNNAC	4 cut(s)	140, 290, 298, 1249
HpyAV	CCTTC	7 cut(s)	30, 166, 355, 701, 1649, 1768, 1830
HpyCH4III	ACNGT	2 cut(s)	700, 1544
HpyCH4IV	ACGT	2 cut(s)	390, 1911
HpyF3I	CTNAG	4 cut(s)	280, 1047, 1071, 1638
HpySE526I	ACGT	2 cut(s)	390, 1911
HspAI	GCGC	3 cut(s)	828, 1337, 1607
KpnI	GGTACC	1 cut(s)	1410
KroI	GCCGGC	1 cut(s)	1741
KroNI	GCCGGC	1 cut(s)	1743
Ksp22I	TGATCA	3 cut(s)	76, 364, 511
Kzo9I	GATC	9 cut(s)	76, 364, 511, 1063, 1174, 1232, 1417, 1546, 1792
LmnI	GCTCC	2 cut(s)	994, 1959
Lsp1109I	GCAGC	5 cut(s)	333, 482, 770, 905, 1340
MaeI	CTAG	9 cut(s)	92, 269, 293, 852, 986, 1113, 1145, 1587, 1979
MaeII	ACGT	2 cut(s)	390, 1911
MaeIII	GTNAC	3 cut(s)	3, 533, 1641
MalI	GATC	9 cut(s)	78, 366, 513, 1065, 1176, 1234, 1419, 1548, 1794
MboI	GATC	9 cut(s)	76, 364, 511, 1063, 1174, 1232, 1417, 1546, 1792
MboII	GAAGA	7 cut(s)	268, 306, 411, 625, 1558, 1793, 1950
MfeI	CAATTG	1 cut(s)	876
MflI	RGATCY	1 cut(s)	1792
MhlI	GDGCHC	1 cut(s)	1251
MlyI	GAGTC	4 cut(s)	33, 384, 410, 1335
Mph1103I	ATGCAT	2 cut(s)	725, 902
MroNI	GCCGGC	1 cut(s)	1741
MroXI	GAANNNNTTC	1 cut(s)	344
MseI	TTAA	3 cut(s)	45, 840, 938
MslI	CAYNNNNRTG	4 cut(s)	531, 1244, 1431, 1830
MspA1I	CMGCKG	2 cut(s)	761, 1484
MspI	CCGG	4 cut(s)	305, 958, 1599, 1742
MspR9I	CCNGG	5 cut(s)	306, 459, 1053, 1626, 1974
MunI	CAATTG	1 cut(s)	876
MvaI	CCWGG	4 cut(s)	459, 1053, 1626, 1974
MvnI	CGCG	2 cut(s)	264, 1339
NaeI	GCCGGC	1 cut(s)	1743
NciI	CCSGG	1 cut(s)	306
NcoI	CCATGG	1 cut(s)	861
NdeI	CATATG	1 cut(s)	922
NdeII	GATC	9 cut(s)	76, 364, 511, 1063, 1174, 1232, 1417, 1546, 1792
NgoMIV	GCCGGC	1 cut(s)	1741
NlaIV	GGNNCC	4 cut(s)	250, 740, 1408, 1794
NmuCI	GTSAC	2 cut(s)	533, 1641
NsiI	ATGCAT	2 cut(s)	725, 902
NspI	RCATGY	4 cut(s)	727, 886, 900, 1831
NspV	TTCGAA	1 cut(s)	2036
OliI	CACNNNNGTG	1 cut(s)	1830
PaeI	GCATGC	1 cut(s)	900
PaeR7I	CTCGAG	2 cut(s)	1412, 1999
PdiI	GCCGGC	1 cut(s)	1743
PdmI	GAANNNNTTC	1 cut(s)	344
PfeI	GAWTC	1 cut(s)	2033
PfoI	TCCNGGA	1 cut(s)	1624
PkrI	GCNGC	8 cut(s)	323, 497, 760, 895, 1041, 1125, 1355, 1483
PleI	GAGTC	4 cut(s)	33, 383, 410, 1335
PpsI	GAGTC	4 cut(s)	33, 383, 410, 1335
Psp6I	CCWGG	4 cut(s)	457, 1051, 1624, 1972
PspFI	CCCAGC	2 cut(s)	1214, 1897
PspGI	CCWGG	4 cut(s)	457, 1051, 1624, 1972
PspN4I	GGNNCC	4 cut(s)	250, 740, 1408, 1794
PspPI	GGNCC	1 cut(s)	1970
PspXI	VCTCGAGB	2 cut(s)	1412, 1999
PstI	CTGCAG	1 cut(s)	1318
PstNI	CAGNNNCTG	1 cut(s)	194
PsuI	RGATCY	1 cut(s)	1792
PvuII	CAGCTG	1 cut(s)	761
RsaI	GTAC	5 cut(s)	329, 404, 1408, 1463, 1929
RsaNI	GTAC	5 cut(s)	328, 403, 1407, 1462, 1928
RseI	CAYNNNNRTG	4 cut(s)	531, 1244, 1431, 1830
SaqAI	TTAA	3 cut(s)	45, 840, 938
SatI	GCNGC	8 cut(s)	322, 496, 759, 894, 1040, 1124, 1354, 1482
Sau3AI	GATC	9 cut(s)	76, 364, 511, 1063, 1174, 1232, 1417, 1546, 1792
Sau96I	GGNCC	1 cut(s)	1970
SchI	GAGTC	4 cut(s)	33, 384, 410, 1335
ScrFI	CCNGG	5 cut(s)	306, 459, 1053, 1626, 1974
SduI	GDGCHC	1 cut(s)	1251
SfcI	CTRYAG	1 cut(s)	1314
Sfr274I	CTCGAG	2 cut(s)	1412, 1999
SfuI	TTCGAA	1 cut(s)	2036
SlaI	CTCGAG	2 cut(s)	1412, 1999
SmiMI	CAYNNNNRTG	4 cut(s)	531, 1244, 1431, 1830
SmlI	CTYRAG	2 cut(s)	1412, 1999
SmoI	CTYRAG	2 cut(s)	1412, 1999
SpeI	ACTAGT	2 cut(s)	292, 851
SphI	GCATGC	1 cut(s)	900
SsiI	CCGC	5 cut(s)	262, 1039, 1123, 1482, 1745
SspI	AATATT	1 cut(s)	1333
SspMI	CTAG	9 cut(s)	92, 269, 293, 852, 986, 1113, 1145, 1587, 1979
StyD4I	CCNGG	5 cut(s)	304, 457, 1051, 1624, 1972
StyI	CCWWGG	4 cut(s)	34, 657, 742, 861
TaaI	ACNGT	2 cut(s)	700, 1544
TaiI	ACGT	2 cut(s)	393, 1914
TaqI	TCGA	6 cut(s)	348, 1013, 1413, 2000, 2021, 2036
TatI	WGTACW	2 cut(s)	327, 1927
TauI	GCSGC	3 cut(s)	1042, 1126, 1484
TfiI	GAWTC	1 cut(s)	2033
Tru1I	TTAA	3 cut(s)	45, 840, 938
Tru9I	TTAA	3 cut(s)	45, 840, 938
TscAI	CASTG	6 cut(s)	645, 705, 1251, 1383, 1547, 1954
TseFI	GTSAC	2 cut(s)	533, 1641
TseI	GCWGC	5 cut(s)	321, 495, 758, 893, 1353
Tsp45I	GTSAC	2 cut(s)	533, 1641
TspDTI	ATGAA	5 cut(s)	144, 988, 1404, 1558, 1935
TspRI	CASTG	6 cut(s)	645, 705, 1251, 1383, 1547, 1954
VneI	GTGCAC	1 cut(s)	1247
XapI	RAATTY	3 cut(s)	272, 1521, 1615
XbaI	TCTAGA	1 cut(s)	91
XceI	RCATGY	4 cut(s)	727, 886, 900, 1831
XcmI	CCANNNNNNNNNTGG	2 cut(s)	134, 586
XhoI	CTCGAG	2 cut(s)	1412, 1999
XmiI	GTMKAC	1 cut(s)	289
XmnI	GAANNNNTTC	1 cut(s)	344
XspI	CTAG	9 cut(s)	92, 269, 293, 852, 986, 1113, 1145, 1587, 1979
Zsp2I	ATGCAT	2 cut(s)	725, 902

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.