Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0016604

Physical Location & Seq

Genomic Coordinates Forward (+)

23640851 .. 23643536

Gene Structure 2686 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1161300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1938 bp

ATGTTACCTCTATTTCTACCTTCCTTTCTTCTCCTTGGACTCCTTAATTCCTTACCATTTTCTCATTCCCTCCATGGCCAACAACAAAAACTAGAACAACCCACTTTGCCTCCCAACCAGCTTTGCAATGAAAAATGTGGACAACTTCAACTACCCTTCCCATTTCACTTGAACAAGTCTTGCAGTTCAGTCTCTGATGCTTTCCACCTCTCTTGCCAAAACTCAACAACCCTTTTCCTCAACATTGGTTCCGAAAGCTACCGGGTGCTAGACTTCTTCTCTGATGGCCTACTAGTGGACTTTCCGGGCTCCTCTTCGTGCCGCCAGTACAACGACTTGAACTCCTTCGATTTCTTGGGAAATGACTATTTCGGAGTTGCGGTTGAAAATGTGGTTGGCTTATATGATTGTGAGGACTCTTCTTTGTGCAAGACAGATTGTGAAACAAATGACTTGCCTGGTTGTGATGGCAACGAGAACAGCTCTCCTGCTTGCTGCTACCCTCTCTCTGATCATAGCGTGTGGCATCTTGGTGACAAGTTTTCGGTTTTTGCCAAGTTTGGATGCAGGGGCATTTCCAGTTGGGTTGTACAAAGAGGGTCTAACTTGGGGAAGAGAGGGGTAAAATTGGAATGGGCAGTGCCAAAGAACTCATCCAAGGAAGTTTGTGATAGCAGTGCTTATATTACTAATGCAACAGTAGTTGAAGGAGGGGTGAGATGCATGTGTCCAGATGGGTTTCTTGGTGATGGCCTTGCAGCTGGTGCAGGGTGCTTGAAATCATGCATCAAGGACAGAAAGGAAGCATATGGCAATGATTGCTTAAAGATAAAGCATGGTGGCCGGAAACTTGCAATTTTAGCCGGAGTTCTAGCTCCAGTTTTCATCATAGCCTCCTTGATTTCACTTTTGTATATACTAAAGCGGCCTGCTAAACCCAGAACATTTGATCCTGCTCGGAAAGTCCATTTTCACAGCACCAAGTCATTCCGAAAAGCTAGTAGGACGCGGCTATTCACGTACCATGAGCTAGAGGAAGCTACCAAAGGGTTTGAGGTGTGTCAGAAACTTGCACATGGAAACAATGGCACAATCTTTGCTGGGGTTCTTGGTGACGGATCGCAAATTGCGGTGCACAAGGTAGAATGTGAGAATGAAAAAGACCTAATTCAAGTCCTATCACAAGTCGAGGTTTTATCTTCTGTTTTGCACCGAAATATCGCGCGATTTATTGGGTGCTGCATTGACTTGGCCTATACTCCACTACTGGTATACGAACATCCTGCGAATGGTACCCTCGAGGATCATTTGCATCAAAACGGAGGACAAAATGTTGCTCTTGACTGGTACAAGAGGCTGAGCATTGCTGCTGAAACAGCAAGTGTTCTTGCCTTTTTGCAATACGAAATTTCTCCTCCCATTTTCCACTGTGATCTCAAATCTGCCTACATCTTCATTGACGATAACTTTTCTAGCAAAGTTGCCGGATTTGGGCTACTGAATTCGAGTCCTGGAGATAGTTCTCAGTCACACAATCACGAAGGTTCGCGCTTTCAGCACAATGATGTTTACGCTTTTGGTGTCATGCTATTTGAGATGATTGCAGGCTCAAATTGCTTGGACTTGCCGGCAGTAGCCCTGCAAAAGATAAGGAGTGGGAAGGTGGAAGAGATTGTGGATCCACTTCTCTACTATCATGAGCAACCTTCGCACAGGCGCGAGCAGATAGAGGTGGTTGCAGACCTTGCCATGAGGTGCCTGTTGTTTGGTGGAGATGGGAAGCTGGGAATGTATGATGTTGTGCGCGAATTAGTACATATTAGAAGGGACAGCAGTGATGGAGGGAGCAAGAGAGGGCCTGGACTAGAGGAAACATTTTCGAACTCGAGCCTGCTTCAGATGATTTCGATGTCTCCTGATTCGAAATATGTGCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase_Tyr

Protein Analysis

646

Amino Acids

70.93

Weight (kDa)

6.03

Isoelectric Point (pI)

45.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GUB_WAK_bind	PF13947	42 - 92	2.3e-09	Wall-associated receptor kinase galacturonan-binding
Pkinase	PF00069	351 - 541	2.2e-13	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	353 - 536	2.2e-17	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016604)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46850
fragaria_vesca	FvH4_7g17870
malus_domestica	MD01G1091100.v1.1 MD07G1161300.v1.1
prunus_persica	Prupe.2G200600_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0361321
rosa_laevigata	RLG00000027725
rosa_roxburghii	Rroxscaffold_4G00295040
rosa_rugosa	Rorug01G0288300
rosa_samantha	Rh1AG299500 Rh1BG263300 Rh1CG280900 Rh1DG293400
rosa_wichuraiana	Rw1G026510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	1298
Acc65I	GGTACC	1 cut(s)	1292
AccB1I	GGYRCC	2 cut(s)	1292, 1755
AccI	GTMKAC	1 cut(s)	1272
AccII	CGCG	6 cut(s)	1009, 1223, 1225, 1549, 1719, 1806
AciI	CCGC	5 cut(s)	322, 380, 925, 1009, 1130
AclWI	GGATC	5 cut(s)	944, 1126, 1311, 1673, 1686
AcoI	YGGCCR	2 cut(s)	76, 841
AcsI	RAATTY	2 cut(s)	1407, 1501
AcuI	CTGAAG	1 cut(s)	1880
AfaI	GTAC	6 cut(s)	329, 591, 1022, 1294, 1349, 1815
AfiI	CCNNNNNNNGG	3 cut(s)	295, 1289, 1491
AgsI	TTSAA	7 cut(s)	149, 172, 340, 386, 707, 778, 1172
AhlI	ACTAGT	1 cut(s)	292
AjnI	CCWGG	3 cut(s)	457, 1510, 1858
AjuI	GAANNNNNNNTTGG	2 cut(s)	378, 410
AloI	GAACNNNNNNTCC	2 cut(s)	934, 966
AluBI	AGCT	9 cut(s)	121, 258, 483, 761, 875, 998, 1030, 1040, 1783
AluI	AGCT	9 cut(s)	121, 258, 483, 761, 875, 998, 1030, 1040, 1783
Alw21I	GWGCWC	1 cut(s)	1137
Alw26I	GTCTC	2 cut(s)	196, 1917
Alw44I	GTGCAC	1 cut(s)	1133
AlwI	GGATC	5 cut(s)	944, 1126, 1311, 1673, 1686
AlwNI	CAGNNNCTG	1 cut(s)	194
Ama87I	CYCGRG	2 cut(s)	1298, 1885
AoxI	GGCC	7 cut(s)	76, 286, 751, 841, 926, 1251, 1856
ApaLI	GTGCAC	1 cut(s)	1133
ApeKI	GCWGC	4 cut(s)	495, 758, 1239, 1367
ApoI	RAATTY	2 cut(s)	1407, 1501
Asp700I	GAANNNNTTC	1 cut(s)	344
Asp718I	GGTACC	1 cut(s)	1292
AspLEI	GCGC	4 cut(s)	1225, 1551, 1719, 1806
AspS9I	GGNCC	1 cut(s)	1856
AsuC2I	CCSGG	2 cut(s)	263, 306
AsuHPI	GGTGA	4 cut(s)	545, 727, 758, 1124
AsuII	TTCGAA	2 cut(s)	1880, 1922
AvaI	CYCGRG	2 cut(s)	1298, 1885
BaeGI	GKGCMC	1 cut(s)	1137
BalI	TGGCCA	1 cut(s)	78
BamHI	GGATCC	1 cut(s)	1678
BanI	GGYRCC	2 cut(s)	1292, 1755
BanII	GRGCYC	1 cut(s)	311
Bbv12I	GWGCWC	1 cut(s)	1137
BbvI	GCAGC	4 cut(s)	482, 770, 1226, 1354
BccI	CCATC	6 cut(s)	278, 461, 728, 743, 1769, 1832
BcgI	CGANNNNNNTGC	3 cut(s)	1265, 1299, 1912
BciT130I	CCWGG	3 cut(s)	459, 1512, 1860
BclI	TGATCA	1 cut(s)	511
BcnI	CCSGG	2 cut(s)	263, 306
BcoDI	GTCTC	2 cut(s)	196, 1917
BcuI	ACTAGT	1 cut(s)	292
BfaI	CTAG	8 cut(s)	92, 269, 293, 872, 999, 1031, 1473, 1865
BisI	GCNGC	7 cut(s)	322, 496, 759, 926, 1010, 1240, 1368
BlpI	GCTNAGC	1 cut(s)	1358
BlsI	GCNGC	7 cut(s)	323, 497, 760, 927, 1011, 1241, 1369
Bme1390I	CCNGG	5 cut(s)	263, 306, 459, 1512, 1860
BmeT110I	CYCGRG	2 cut(s)	1298, 1885
BmgT120I	GGNCC	1 cut(s)	1856
BmiI	GGNNCC	5 cut(s)	250, 310, 1294, 1680, 1757
BmrFI	CCNGG	5 cut(s)	263, 306, 459, 1512, 1860
BmsI	GCATC	6 cut(s)	187, 535, 554, 710, 795, 1321
BplI	GAGNNNNNCTC	2 cut(s)	467, 499
BpmI	CTGGAG	2 cut(s)	861, 1533
Bpu1102I	GCTNAGC	1 cut(s)	1358
Bpu14I	TTCGAA	2 cut(s)	1880, 1922
BpuMI	CCSGG	2 cut(s)	263, 306
BsaAI	YACGTR	1 cut(s)	1020
BsaJI	CCNNGG	3 cut(s)	34, 73, 657
BsaXI	ACNNNNNCTCC	6 cut(s)	94, 124, 326, 356, 366, 396
Bsc4I	CCNNNNNNNGG	3 cut(s)	295, 1289, 1491
Bse118I	RCCGGY	1 cut(s)	1627
Bse1I	ACTGG	5 cut(s)	325, 579, 878, 1272, 1349
Bse3DI	GCAATG	3 cut(s)	133, 820, 1362
BseBI	CCWGG	3 cut(s)	459, 1512, 1860
BseDI	CCNNGG	3 cut(s)	34, 73, 657
BseGI	GGATG	3 cut(s)	569, 653, 1279
BseLI	CCNNNNNNNGG	3 cut(s)	295, 1289, 1491
BseMI	GCAATG	3 cut(s)	133, 820, 1362
BseMII	CTCAG	2 cut(s)	1349, 1538
BseNI	ACTGG	5 cut(s)	325, 579, 878, 1272, 1349
BseRI	GAGGAG	2 cut(s)	301, 1404
BseSI	GKGCMC	1 cut(s)	1137
BseXI	GCAGC	4 cut(s)	482, 770, 1226, 1354
BseYI	CCCAGC	2 cut(s)	1100, 1783
BsgI	GTGCAG	1 cut(s)	786
Bsh1236I	CGCG	6 cut(s)	1009, 1223, 1225, 1549, 1719, 1806
BshFI	GGCC	7 cut(s)	78, 288, 753, 843, 928, 1253, 1858
BshNI	GGYRCC	2 cut(s)	1292, 1755
BsiHKAI	GWGCWC	1 cut(s)	1137
BsiHKCI	CYCGRG	2 cut(s)	1298, 1885
BsiSI	CCGG	6 cut(s)	262, 305, 844, 864, 1485, 1628
BslFI	GGGAC	1 cut(s)	1841
BslI	CCNNNNNNNGG	3 cut(s)	295, 1289, 1491
BsmAI	GTCTC	2 cut(s)	196, 1917
BsmFI	GGGAC	1 cut(s)	1841
BsnI	GGCC	7 cut(s)	78, 288, 753, 843, 928, 1253, 1858
BsoBI	CYCGRG	2 cut(s)	1298, 1885
Bsp119I	TTCGAA	2 cut(s)	1880, 1922
Bsp1286I	GDGCHC	2 cut(s)	311, 1137
Bsp1407I	TGTACA	1 cut(s)	589
Bsp143I	GATC	6 cut(s)	511, 949, 1118, 1303, 1432, 1678
Bsp1720I	GCTNAGC	1 cut(s)	1358
Bsp19I	CCATGG	1 cut(s)	73
BspACI	CCGC	5 cut(s)	322, 380, 925, 1009, 1130
BspANI	GGCC	7 cut(s)	78, 288, 753, 843, 928, 1253, 1858
BspCNI	CTCAG	2 cut(s)	1350, 1537
BspFNI	CGCG	6 cut(s)	1009, 1223, 1225, 1549, 1719, 1806
BspHI	TCATGA	1 cut(s)	1696
BspLI	GGNNCC	5 cut(s)	250, 310, 1294, 1680, 1757
BspPI	GGATC	5 cut(s)	944, 1126, 1311, 1673, 1686
BspT104I	TTCGAA	2 cut(s)	1880, 1922
BspT107I	GGYRCC	2 cut(s)	1292, 1755
BsrDI	GCAATG	3 cut(s)	133, 820, 1362
BsrFI	RCCGGY	1 cut(s)	1627
BsrGI	TGTACA	1 cut(s)	589
BsrI	ACTGG	5 cut(s)	325, 579, 878, 1272, 1349
BssAI	RCCGGY	1 cut(s)	1627
BssECI	CCNNGG	3 cut(s)	34, 73, 657
BssMI	GATC	6 cut(s)	511, 949, 1118, 1303, 1432, 1678
BssNAI	GTATAC	1 cut(s)	1273
BssT1I	CCWWGG	3 cut(s)	34, 73, 657
Bst1107I	GTATAC	1 cut(s)	1273
Bst2UI	CCWGG	3 cut(s)	459, 1512, 1860
Bst4CI	ACNGT	2 cut(s)	700, 1430
Bst6I	CTCTTC	4 cut(s)	319, 424, 608, 1662
BstAPI	GCANNNNNTGC	3 cut(s)	764, 819, 1745
BstAUI	TGTACA	1 cut(s)	589
BstBAI	YACGTR	1 cut(s)	1020
BstBI	TTCGAA	2 cut(s)	1880, 1922
BstC8I	GCNNGC	6 cut(s)	493, 930, 1606, 1629, 1721, 1892
BstDEI	CTNAG	2 cut(s)	1358, 1524
BstDSI	CCRYGG	1 cut(s)	73
BstF5I	GGATG	3 cut(s)	569, 653, 1279
BstFNI	CGCG	6 cut(s)	1009, 1223, 1225, 1549, 1719, 1806
BstHHI	GCGC	4 cut(s)	1225, 1551, 1719, 1806
BstKTI	GATC	6 cut(s)	514, 952, 1121, 1306, 1435, 1681
BstMAI	GTCTC	2 cut(s)	196, 1917
BstMBI	GATC	6 cut(s)	511, 949, 1118, 1303, 1432, 1678
BstMWI	GCNNNNNNNGC	8 cut(s)	764, 819, 860, 1376, 1555, 1614, 1708, 1745
BstNI	CCWGG	3 cut(s)	459, 1512, 1860
BstNSI	RCATGY	1 cut(s)	727
BstSCI	CCNGG	5 cut(s)	261, 304, 457, 1510, 1858
BstSLI	GKGCMC	1 cut(s)	1137
BstUI	CGCG	6 cut(s)	1009, 1223, 1225, 1549, 1719, 1806
BstV1I	GCAGC	4 cut(s)	482, 770, 1226, 1354
BstX2I	RGATCY	1 cut(s)	1678
BstYI	RGATCY	1 cut(s)	1678
BstZ17I	GTATAC	1 cut(s)	1273
BsuRI	GGCC	7 cut(s)	78, 288, 753, 843, 928, 1253, 1858
BtgI	CCRYGG	1 cut(s)	73
BtsCI	GGATG	3 cut(s)	569, 653, 1279
BtsI	GCAGTG	3 cut(s)	645, 682, 1840
BtsIMutI	CAGTG	4 cut(s)	645, 682, 1426, 1840
Cac8I	GCNNGC	6 cut(s)	493, 930, 1606, 1629, 1721, 1892
CaiI	CAGNNNCTG	1 cut(s)	194
CciI	TCATGA	1 cut(s)	1696
CfoI	GCGC	4 cut(s)	1225, 1551, 1719, 1806
Cfr10I	RCCGGY	1 cut(s)	1627
Cfr13I	GGNCC	1 cut(s)	1856
CseI	GACGC	1 cut(s)	1015
Csp6I	GTAC	6 cut(s)	328, 590, 1021, 1293, 1348, 1814
CspCI	CAANNNNNGTGG	2 cut(s)	194, 229
CviAII	CATG	9 cut(s)	74, 724, 783, 836, 1025, 1076, 1585, 1697, 1750
CviQI	GTAC	6 cut(s)	328, 590, 1021, 1293, 1348, 1814
DdeI	CTNAG	2 cut(s)	1358, 1524
DpnI	GATC	6 cut(s)	513, 951, 1120, 1305, 1434, 1680
DpnII	GATC	6 cut(s)	511, 949, 1118, 1303, 1432, 1678
EaeI	YGGCCR	2 cut(s)	76, 841
Eam1104I	CTCTTC	4 cut(s)	319, 424, 608, 1662
EarI	CTCTTC	4 cut(s)	319, 424, 608, 1662
Eco130I	CCWWGG	3 cut(s)	34, 73, 657
Eco24I	GRGCYC	1 cut(s)	311
Eco57I	CTGAAG	1 cut(s)	1880
Eco88I	CYCGRG	2 cut(s)	1298, 1885
EcoO109I	RGGNCCY	1 cut(s)	1856
EcoRI	GAATTC	1 cut(s)	1501
EcoRII	CCWGG	3 cut(s)	457, 1510, 1858
EcoT14I	CCWWGG	3 cut(s)	34, 73, 657
EcoT22I	ATGCAT	2 cut(s)	725, 788
EcoT38I	GRGCYC	1 cut(s)	311
ErhI	CCWWGG	3 cut(s)	34, 73, 657
FaeI	CATG	9 cut(s)	77, 727, 786, 839, 1028, 1079, 1588, 1700, 1753
FaqI	GGGAC	1 cut(s)	1841
FatI	CATG	9 cut(s)	73, 723, 782, 835, 1024, 1075, 1584, 1696, 1749
FauNDI	CATATG	1 cut(s)	808
FbaI	TGATCA	1 cut(s)	511
FblI	GTMKAC	1 cut(s)	1272
Fnu4HI	GCNGC	7 cut(s)	322, 496, 759, 926, 1010, 1240, 1368
FokI	GGATG	3 cut(s)	576, 640, 1266
FriOI	GRGCYC	1 cut(s)	311
Fsp4HI	GCNGC	7 cut(s)	322, 496, 759, 926, 1010, 1240, 1368
FspBI	CTAG	8 cut(s)	92, 269, 293, 872, 999, 1031, 1473, 1865
GlaI	GCGC	4 cut(s)	1224, 1550, 1718, 1805
GluI	GCNGC	7 cut(s)	322, 496, 759, 926, 1010, 1240, 1368
GsaI	CCCAGC	2 cut(s)	1104, 1787
GsuI	CTGGAG	2 cut(s)	861, 1533
HaeIII	GGCC	7 cut(s)	78, 288, 753, 843, 928, 1253, 1858
HapII	CCGG	6 cut(s)	262, 305, 844, 864, 1485, 1628
HgaI	GACGC	1 cut(s)	1015
HhaI	GCGC	4 cut(s)	1225, 1551, 1719, 1806
Hin1II	CATG	9 cut(s)	77, 727, 786, 839, 1028, 1079, 1588, 1700, 1753
Hin6I	GCGC	4 cut(s)	1223, 1549, 1717, 1804
HinP1I	GCGC	4 cut(s)	1223, 1549, 1717, 1804
HinfI	GANTC	4 cut(s)	39, 416, 1507, 1919
HpaII	CCGG	6 cut(s)	262, 305, 844, 864, 1485, 1628
HphI	GGTGA	4 cut(s)	545, 727, 758, 1124
Hpy166II	GTNNAC	5 cut(s)	140, 298, 1135, 1273, 1570
Hpy188I	TCNGA	9 cut(s)	196, 253, 283, 374, 511, 960, 992, 1065, 1899
Hpy188III	TCNNGA	5 cut(s)	731, 1340, 1538, 1697, 1916
Hpy8I	GTNNAC	5 cut(s)	140, 298, 1135, 1273, 1570
HpyAV	CCTTC	8 cut(s)	30, 166, 355, 701, 1535, 1654, 1716, 1818
HpyCH4III	ACNGT	2 cut(s)	700, 1430
HpyCH4IV	ACGT	1 cut(s)	1019
HpyF10VI	GCNNNNNNNGC	8 cut(s)	764, 819, 860, 1376, 1555, 1614, 1708, 1745
HpyF3I	CTNAG	2 cut(s)	1358, 1524
HpySE526I	ACGT	1 cut(s)	1019
Hsp92II	CATG	9 cut(s)	77, 727, 786, 839, 1028, 1079, 1588, 1700, 1753
HspAI	GCGC	4 cut(s)	1223, 1549, 1717, 1804
KpnI	GGTACC	1 cut(s)	1296
KroI	GCCGGC	1 cut(s)	1627
KroNI	GCCGGC	1 cut(s)	1629
Ksp22I	TGATCA	1 cut(s)	511
Kzo9I	GATC	6 cut(s)	511, 949, 1118, 1303, 1432, 1678
LmnI	GCTCC	3 cut(s)	314, 880, 1845
Lsp1109I	GCAGC	4 cut(s)	482, 770, 1226, 1354
LweI	GCATC	6 cut(s)	187, 535, 554, 710, 795, 1321
MaeI	CTAG	8 cut(s)	92, 269, 293, 872, 999, 1031, 1473, 1865
MaeII	ACGT	1 cut(s)	1019
MaeIII	GTNAC	4 cut(s)	3, 533, 1112, 1527
MalI	GATC	6 cut(s)	513, 951, 1120, 1305, 1434, 1680
MboI	GATC	6 cut(s)	511, 949, 1118, 1303, 1432, 1678
MboII	GAAGA	8 cut(s)	20, 268, 306, 411, 625, 1191, 1444, 1679
MflI	RGATCY	1 cut(s)	1678
MhlI	GDGCHC	2 cut(s)	311, 1137
MlsI	TGGCCA	1 cut(s)	78
MluCI	AATT	9 cut(s)	46, 626, 855, 1125, 1167, 1407, 1501, 1612, 1808
MluNI	TGGCCA	1 cut(s)	78
MlyI	GAGTC	3 cut(s)	33, 410, 1516
Mox20I	TGGCCA	1 cut(s)	78
Mph1103I	ATGCAT	2 cut(s)	725, 788
MroNI	GCCGGC	1 cut(s)	1627
MroXI	GAANNNNTTC	1 cut(s)	344
MscI	TGGCCA	1 cut(s)	78
MseI	TTAA	2 cut(s)	45, 824
MslI	CAYNNNNRTG	2 cut(s)	531, 1563
Msp20I	TGGCCA	1 cut(s)	78
MspA1I	CMGCKG	1 cut(s)	761
MspI	CCGG	6 cut(s)	262, 305, 844, 864, 1485, 1628
MspR9I	CCNGG	5 cut(s)	263, 306, 459, 1512, 1860
MvaI	CCWGG	3 cut(s)	459, 1512, 1860
MvnI	CGCG	6 cut(s)	1009, 1223, 1225, 1549, 1719, 1806
MwoI	GCNNNNNNNGC	8 cut(s)	764, 819, 860, 1376, 1555, 1614, 1708, 1745
NaeI	GCCGGC	1 cut(s)	1629
NciI	CCSGG	2 cut(s)	263, 306
NcoI	CCATGG	1 cut(s)	73
NdeI	CATATG	1 cut(s)	808
NdeII	GATC	6 cut(s)	511, 949, 1118, 1303, 1432, 1678
NgoMIV	GCCGGC	1 cut(s)	1627
NlaIII	CATG	9 cut(s)	77, 727, 786, 839, 1028, 1079, 1588, 1700, 1753
NlaIV	GGNNCC	5 cut(s)	250, 310, 1294, 1680, 1757
NmuCI	GTSAC	3 cut(s)	533, 1112, 1527
NsiI	ATGCAT	2 cut(s)	725, 788
NspI	RCATGY	1 cut(s)	727
NspV	TTCGAA	2 cut(s)	1880, 1922
PaeR7I	CTCGAG	2 cut(s)	1298, 1885
PagI	TCATGA	1 cut(s)	1696
PdiI	GCCGGC	1 cut(s)	1629
PdmI	GAANNNNTTC	1 cut(s)	344
PfeI	GAWTC	1 cut(s)	1919
PfoI	TCCNGGA	1 cut(s)	1510
PkrI	GCNGC	7 cut(s)	323, 497, 760, 927, 1011, 1241, 1369
PleI	GAGTC	3 cut(s)	33, 410, 1515
PpsI	GAGTC	3 cut(s)	33, 410, 1515
Ppu21I	YACGTR	1 cut(s)	1020
Psp6I	CCWGG	3 cut(s)	457, 1510, 1858
PspFI	CCCAGC	2 cut(s)	1100, 1783
PspGI	CCWGG	3 cut(s)	457, 1510, 1858
PspN4I	GGNNCC	5 cut(s)	250, 310, 1294, 1680, 1757
PspPI	GGNCC	1 cut(s)	1856
PspXI	VCTCGAGB	2 cut(s)	1298, 1885
PstNI	CAGNNNCTG	1 cut(s)	194
PsuI	RGATCY	1 cut(s)	1678
PvuII	CAGCTG	1 cut(s)	761
RsaI	GTAC	6 cut(s)	329, 591, 1022, 1294, 1349, 1815
RsaNI	GTAC	6 cut(s)	328, 590, 1021, 1293, 1348, 1814
RseI	CAYNNNNRTG	2 cut(s)	531, 1563
SaqAI	TTAA	2 cut(s)	45, 824
SatI	GCNGC	7 cut(s)	322, 496, 759, 926, 1010, 1240, 1368
Sau3AI	GATC	6 cut(s)	511, 949, 1118, 1303, 1432, 1678
Sau96I	GGNCC	1 cut(s)	1856
SchI	GAGTC	3 cut(s)	33, 410, 1516
ScrFI	CCNGG	5 cut(s)	263, 306, 459, 1512, 1860
SduI	GDGCHC	2 cut(s)	311, 1137
SfaNI	GCATC	6 cut(s)	187, 535, 554, 710, 795, 1321
Sfr274I	CTCGAG	2 cut(s)	1298, 1885
SfuI	TTCGAA	2 cut(s)	1880, 1922
SlaI	CTCGAG	2 cut(s)	1298, 1885
SmiMI	CAYNNNNRTG	2 cut(s)	531, 1563
SmlI	CTYRAG	2 cut(s)	1298, 1885
SmoI	CTYRAG	2 cut(s)	1298, 1885
SpeI	ACTAGT	1 cut(s)	292
Sse9I	AATT	9 cut(s)	46, 626, 855, 1125, 1167, 1407, 1501, 1612, 1808
SsiI	CCGC	5 cut(s)	322, 380, 925, 1009, 1130
SspMI	CTAG	8 cut(s)	92, 269, 293, 872, 999, 1031, 1473, 1865
StyD4I	CCNGG	5 cut(s)	261, 304, 457, 1510, 1858
StyI	CCWWGG	3 cut(s)	34, 73, 657
TaaI	ACNGT	2 cut(s)	700, 1430
TaiI	ACGT	1 cut(s)	1022
TaqI	TCGA	8 cut(s)	348, 1188, 1299, 1505, 1880, 1886, 1907, 1922
TasI	AATT	9 cut(s)	46, 626, 855, 1125, 1167, 1407, 1501, 1612, 1808
TatI	WGTACW	3 cut(s)	327, 589, 1813
TauI	GCSGC	3 cut(s)	324, 928, 1012
TfiI	GAWTC	1 cut(s)	1919
Tru1I	TTAA	2 cut(s)	45, 824
Tru9I	TTAA	2 cut(s)	45, 824
TscAI	CASTG	4 cut(s)	645, 682, 1433, 1840
TseFI	GTSAC	3 cut(s)	533, 1112, 1527
TseI	GCWGC	4 cut(s)	495, 758, 1239, 1367
Tsp45I	GTSAC	3 cut(s)	533, 1112, 1527
TspDTI	ATGAA	4 cut(s)	144, 874, 1170, 1444
TspGWI	ACGGA	2 cut(s)	1131, 1335
TspRI	CASTG	4 cut(s)	645, 682, 1433, 1840
VneI	GTGCAC	1 cut(s)	1133
XapI	RAATTY	2 cut(s)	1407, 1501
XceI	RCATGY	1 cut(s)	727
XhoI	CTCGAG	2 cut(s)	1298, 1885
XmiI	GTMKAC	1 cut(s)	1272
XmnI	GAANNNNTTC	1 cut(s)	344
XspI	CTAG	8 cut(s)	92, 269, 293, 872, 999, 1031, 1473, 1865
Zsp2I	ATGCAT	2 cut(s)	725, 788

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.