Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0013206

Physical Location & Seq

Genomic Coordinates Forward (+)

23428683 .. 23434574

Gene Structure 5892 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1120700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGTCCTCCTTGTTCCTTATCCTGTTCTTACTCTCCCACTCCCTCTGTGTTTTTGTTACTTCAGCCGGCAAAGTTGACGACTCTCGGACGTTATTATGGCCGCTGCCGGCCAAGTTCACCTTCGGGAACAAGACCCTATCCGTCGACCCGGCGCTGTCGCTGGTTGTTGGCGGGAGTGGCGGCGGCTCCGGCATTCTGAAGCTGGGTTTTGATCGGTACAGAGAGATTATATTCGAGAACAGTCATGAGGTTTTAGCTTTGAATACACTGAGGGGAAAGAGACAGAGTTTTGATATTAATAAGCTGAGAATTGTGGTTCAATCGAGCAACGAAGATCTGCAACTTGGTGTGGATGAGAGCTATACTTTGTTTGTGGCGAAGAAAGATGGGACATCGGTTGTTGGGGAGGCGACGATCGAGGCGAACACTGTGTATGGAGCTCTGCGGGCTTTGGAGACATTCAGCCAGTTGTGTACTTTTGATTACGGGAGTAAATCTGTACAAGTATATAAGGCACCCTGGTACATAAAGGACAGTCCAAGGTTTCAATACCGAGGGCTTTTGATTGATACATCAAGGCACTATTTACCAATTGAAGTGATTAAGCAAATAATTCAGTCGATGTCGTATGCTAAACTTAATGTTCTTCATTGGCACGTCATAGATAGAGAGGCTTTTCCTCTAGAAGTACCTTCATATCCAAAGTTGTGGAACGGAGCATACACAAAATGGGAGCGCTACACGGTTGAGGATGCAATTGAAATCGTCAACTTTGCCAAAACAAGAGGCGTCAATGTTATGGCAGAAGTAGATGTCCCTGGTCATGCAGCGTCTTGGGGCACCGGGTATCCTGATCTTTGGCCTTCCCCTTCCTGCAAAGAGCCACTTGATGTTTCGAAGGAATTAAGTTTAGATGTGATTTCTGGAATTCTGACAGATATGAGAAAGATTTTTCCCTTCGAGCTGTTCCACTTGGGCGGTGATGAAGTTGATACAACTTGTTGGAGCACCACTCGACATGTGAAGCAATGGCTTAAAGAGCGGAACATGACTACTAAAGATGCATATCAGTATTTCGTTGTCAAGGCCCAAGAAGTAGCAGTTTCAAAAAATTGGTCCCCAGTAAACTGGGAAGAAACCTTCGATACCTTCCCAACGAAGCTCAATCCCAAGACCGTAGTGCATAACTGGTTGGGCTCAGGAGTTTGTCCGAAGGCTGTTGCCAAAGGTTTCAGATGTATTTTTAGCAATCAGGGTGTTTGGTATCTTGACCATCTAGATGTCCCTTGGAATGTTACTTACAATGCTGAACCACTTGAAGGAATTACGGATATTTCTCAGCAGAAGCTTGTCATTGGAGGAGAAGTATGCATGTGGGGTGAGAAAGCCGATACATCAGATGTTCAGCAAACAATTTGGCCTCGAGCAGCAGCTGCTGCAGAACGCTTGTGGAGCAGGAGGGAGGCAACATCTGGTGGAAAGAATATCGCAACAGCACTGCCTCGGCTACAATACTTTAGATGTCTCTTAAATAGACGTGGAGTTCAAGCCGCTCCGGTGACGAACTTCTATGCTCGAAGTCCTCCAACTTGGTCTGGATCATGCTACGATCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004563 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0009505 GO:0015929 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00511 ko00513 ko00520 ko00531 ko00603 ko00604 ko01100 ko04142 map00511 map00513 map00520 map00531 map00603 map00604 map01100 map04142

Pfam Domains

Protein Families

Glyco_hydro_20 Glycohydro_20b2

Protein Analysis

539

Amino Acids

60.56

Weight (kDa)

7.12

Isoelectric Point (pI)

43.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glycohydro_20b2	PF14845	32 - 157	3.5e-24	beta-acetyl hexosaminidase like
Glyco_hydro_20	PF00728	182 - 486	3.6e-78	Glycosyl hydrolase family 20, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013206)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65590 AT3G55260
fragaria_vesca	FvH4_7g21240
malus_domestica	MD01G1120700.v1.1 MD07G1190100.v1.1
prunus_persica	Prupe.2G227600_v2.0.a1 Prupe.2G227600_v2.0.a1 Prupe.2G228000_v2.0.a1
pyrus_communis	pycom01g14730 pycom07g17970
rosa_chinensis	RchiOBHm_Chr1g0365681
rosa_laevigata	RLG00000027418
rosa_roxburghii	Rroxscaffold_4G00291090
rosa_samantha	Rh1AG331300 Rh1BG292800 Rh1CG309300 Rh1DG324300
rosa_wichuraiana	Rw1G029430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	514, 839
AccBSI	CCGCTC	2 cut(s)	1042, 1553
AccI	GTMKAC	1 cut(s)	144
AciI	CCGC	8 cut(s)	101, 171, 180, 183, 445, 978, 1042, 1551
AclWI	GGATC	1 cut(s)	1606
AcoI	YGGCCR	2 cut(s)	98, 108
AcsI	RAATTY	1 cut(s)	927
AcuI	CTGAAG	2 cut(s)	45, 218
AcyI	GRCGYC	1 cut(s)	789
AfaI	GTAC	5 cut(s)	218, 475, 501, 524, 690
AfeI	AGCGCT	1 cut(s)	737
AfiI	CCNNNNNNNGG	1 cut(s)	1319
AflIII	ACRYGT	1 cut(s)	1018
AgsI	TTSAA	8 cut(s)	262, 320, 548, 596, 761, 1107, 1319, 1547
AjiI	CACGTC	2 cut(s)	658, 1538
AjnI	CCWGG	2 cut(s)	518, 817
AjuI	GAANNNNNNNTTGG	2 cut(s)	104, 136
AluBI	AGCT	9 cut(s)	202, 257, 304, 360, 440, 964, 1162, 1348, 1433
AluI	AGCT	9 cut(s)	202, 257, 304, 360, 440, 964, 1162, 1348, 1433
Alw21I	GWGCWC	2 cut(s)	442, 1010
Alw26I	GTCTC	3 cut(s)	274, 449, 1529
AlwI	GGATC	1 cut(s)	1606
AlwNI	CAGNNNCTG	2 cut(s)	1433, 1436
Ama87I	CYCGRG	1 cut(s)	1422
Aor51HI	AGCGCT	1 cut(s)	737
AoxI	GGCC	5 cut(s)	98, 108, 860, 1086, 1418
ApeKI	GCWGC	6 cut(s)	103, 827, 1427, 1430, 1433, 1436
ApoI	RAATTY	1 cut(s)	927
ArsI	GACNNNNNNTTYG	2 cut(s)	1100, 1132
AseI	ATTAAT	1 cut(s)	297
AspLEI	GCGC	2 cut(s)	154, 738
AspS9I	GGNCC	2 cut(s)	1087, 1116
AsuC2I	CCSGG	2 cut(s)	149, 844
AsuHPI	GGTGA	4 cut(s)	109, 992, 1391, 1570
AsuII	TTCGAA	1 cut(s)	896
AvaI	CYCGRG	1 cut(s)	1422
AvaII	GGWCC	1 cut(s)	1116
BaeGI	GKGCMC	1 cut(s)	842
BanI	GGYRCC	2 cut(s)	514, 839
BanII	GRGCYC	2 cut(s)	442, 1199
Bbv12I	GWGCWC	2 cut(s)	442, 1010
BbvI	GCAGC	6 cut(s)	90, 839, 1420, 1423, 1439, 1442
BccI	CCATC	2 cut(s)	380, 1281
BciT130I	CCWGG	2 cut(s)	520, 819
BciVI	GTATCC	1 cut(s)	858
BcnI	CCSGG	2 cut(s)	149, 844
BcoDI	GTCTC	3 cut(s)	274, 449, 1529
BfaI	CTAG	2 cut(s)	683, 1277
BfmI	CTRYAG	1 cut(s)	1437
BfoI	RGCGCY	2 cut(s)	155, 739
BfuI	GTATCC	1 cut(s)	858
BglII	AGATCT	1 cut(s)	334
Bme1390I	CCNGG	4 cut(s)	149, 520, 819, 844
Bme18I	GGWCC	1 cut(s)	1116
BmeT110I	CYCGRG	1 cut(s)	1422
BmgBI	CACGTC	2 cut(s)	658, 1538
BmgT120I	GGNCC	2 cut(s)	1087, 1116
BmiI	GGNNCC	4 cut(s)	187, 516, 841, 1118
BmrFI	CCNGG	4 cut(s)	149, 520, 819, 844
BmrI	ACTGGG	2 cut(s)	1115, 1138
BmsI	GCATC	2 cut(s)	742, 1051
BmuI	ACTGGG	2 cut(s)	1115, 1138
BplI	GAGNNNNNCTC	2 cut(s)	997, 1029
Bpu10I	CCTNAGC	1 cut(s)	1198
Bpu14I	TTCGAA	1 cut(s)	896
BpuMI	CCSGG	2 cut(s)	149, 844
BsaBI	GATNNNNATC	1 cut(s)	1065
BsaHI	GRCGYC	1 cut(s)	789
BsaJI	CCNNGG	6 cut(s)	518, 539, 553, 817, 1286, 1502
BsaWI	WCCGGW	1 cut(s)	1555
BsaXI	ACNNNNNCTCC	2 cut(s)	1350, 1380
Bsc4I	CCNNNNNNNGG	1 cut(s)	1319
Bse118I	RCCGGY	2 cut(s)	65, 106
Bse1I	ACTGG	4 cut(s)	466, 1121, 1133, 1193
Bse3DI	GCAATG	1 cut(s)	1034
Bse8I	GATNNNNATC	1 cut(s)	1065
BseBI	CCWGG	2 cut(s)	520, 819
BseDI	CCNNGG	6 cut(s)	518, 539, 553, 817, 1286, 1502
BseGI	GGATG	2 cut(s)	358, 757
BseJI	GATNNNNATC	1 cut(s)	1065
BseLI	CCNNNNNNNGG	1 cut(s)	1319
BseMI	GCAATG	1 cut(s)	1034
BseMII	CTCAG	4 cut(s)	260, 296, 1212, 1352
BseNI	ACTGG	4 cut(s)	466, 1121, 1133, 1193
BseRI	GAGGAG	1 cut(s)	1374
BseSI	GKGCMC	1 cut(s)	842
BseXI	GCAGC	6 cut(s)	90, 839, 1420, 1423, 1439, 1442
BseYI	CCCAGC	1 cut(s)	202
Bsh1285I	CGRYCG	1 cut(s)	417
BshFI	GGCC	5 cut(s)	100, 110, 862, 1088, 1420
BshNI	GGYRCC	2 cut(s)	514, 839
BsiEI	CGRYCG	1 cut(s)	417
BsiHKAI	GWGCWC	2 cut(s)	442, 1010
BsiHKCI	CYCGRG	1 cut(s)	1422
BsiSI	CCGG	6 cut(s)	66, 107, 149, 189, 843, 1556
BslFI	GGGAC	4 cut(s)	403, 800, 1102, 1268
BslI	CCNNNNNNNGG	1 cut(s)	1319
BsmAI	GTCTC	3 cut(s)	274, 449, 1529
BsmFI	GGGAC	4 cut(s)	403, 800, 1102, 1268
BsmI	GAATGC	1 cut(s)	192
BsnI	GGCC	5 cut(s)	100, 110, 862, 1088, 1420
BsoBI	CYCGRG	1 cut(s)	1422
Bsp119I	TTCGAA	1 cut(s)	896
Bsp1286I	GDGCHC	4 cut(s)	442, 842, 1010, 1199
Bsp1407I	TGTACA	1 cut(s)	499
Bsp143I	GATC	6 cut(s)	211, 334, 414, 853, 1598, 1609
BspACI	CCGC	8 cut(s)	101, 171, 180, 183, 445, 978, 1042, 1551
BspANI	GGCC	5 cut(s)	100, 110, 862, 1088, 1420
BspCNI	CTCAG	4 cut(s)	261, 297, 1211, 1351
BspHI	TCATGA	1 cut(s)	244
BspLI	GGNNCC	4 cut(s)	187, 516, 841, 1118
BspMAI	CTGCAG	1 cut(s)	1441
BspPI	GGATC	1 cut(s)	1606
BspT104I	TTCGAA	1 cut(s)	896
BspT107I	GGYRCC	2 cut(s)	514, 839
BsrBI	CCGCTC	2 cut(s)	1042, 1553
BsrDI	GCAATG	1 cut(s)	1034
BsrFI	RCCGGY	2 cut(s)	65, 106
BsrGI	TGTACA	1 cut(s)	499
BsrI	ACTGG	4 cut(s)	466, 1121, 1133, 1193
BssAI	RCCGGY	2 cut(s)	65, 106
BssECI	CCNNGG	6 cut(s)	518, 539, 553, 817, 1286, 1502
BssMI	GATC	6 cut(s)	211, 334, 414, 853, 1598, 1609
BssNI	GRCGYC	1 cut(s)	789
BssT1I	CCWWGG	2 cut(s)	539, 1286
Bst2UI	CCWGG	2 cut(s)	520, 819
Bst4CI	ACNGT	5 cut(s)	242, 430, 536, 745, 1177
BstACI	GRCGYC	1 cut(s)	789
BstAPI	GCANNNNNTGC	2 cut(s)	1433, 1436
BstAUI	TGTACA	1 cut(s)	499
BstBI	TTCGAA	1 cut(s)	896
BstC8I	GCNNGC	3 cut(s)	67, 108, 447
BstDEI	CTNAG	4 cut(s)	269, 305, 1198, 1338
BstF5I	GGATG	2 cut(s)	358, 757
BstH2I	RGCGCY	2 cut(s)	155, 739
BstHHI	GCGC	2 cut(s)	154, 738
BstKTI	GATC	6 cut(s)	214, 337, 417, 856, 1601, 1612
BstMAI	GTCTC	3 cut(s)	274, 449, 1529
BstMBI	GATC	6 cut(s)	211, 334, 414, 853, 1598, 1609
BstMCI	CGRYCG	1 cut(s)	417
BstMWI	GCNNNNNNNGC	7 cut(s)	177, 189, 446, 1039, 1433, 1436, 1452
BstNI	CCWGG	2 cut(s)	520, 819
BstNSI	RCATGY	2 cut(s)	1022, 1375
BstSCI	CCNGG	4 cut(s)	147, 518, 817, 842
BstSFI	CTRYAG	1 cut(s)	1437
BstSLI	GKGCMC	1 cut(s)	842
BstV1I	GCAGC	6 cut(s)	90, 839, 1420, 1423, 1439, 1442
BstX2I	RGATCY	1 cut(s)	334
BstXI	CCANNNNNNTGG	2 cut(s)	708, 1128
BstYI	RGATCY	1 cut(s)	334
BsuI	GTATCC	1 cut(s)	858
BsuRI	GGCC	5 cut(s)	100, 110, 862, 1088, 1420
BtrI	CACGTC	2 cut(s)	658, 1538
BtsCI	GGATG	2 cut(s)	358, 757
BtsI	GCAGTG	1 cut(s)	1496
BtsIMutI	CAGTG	3 cut(s)	266, 426, 1496
Cac8I	GCNNGC	3 cut(s)	67, 108, 447
CaiI	CAGNNNCTG	2 cut(s)	1433, 1436
CciI	TCATGA	1 cut(s)	244
CfoI	GCGC	2 cut(s)	154, 738
Cfr10I	RCCGGY	2 cut(s)	65, 106
Cfr13I	GGNCC	2 cut(s)	1087, 1116
CseI	GACGC	2 cut(s)	778, 819
Csp6I	GTAC	5 cut(s)	217, 474, 500, 523, 689
CviAII	CATG	6 cut(s)	245, 824, 1019, 1048, 1372, 1602
CviQI	GTAC	5 cut(s)	217, 474, 500, 523, 689
DdeI	CTNAG	4 cut(s)	269, 305, 1198, 1338
DpnI	GATC	6 cut(s)	213, 336, 416, 855, 1600, 1611
DpnII	GATC	6 cut(s)	211, 334, 414, 853, 1598, 1609
EaeI	YGGCCR	2 cut(s)	98, 108
Ecl136II	GAGCTC	1 cut(s)	440
Eco130I	CCWWGG	2 cut(s)	539, 1286
Eco24I	GRGCYC	2 cut(s)	442, 1199
Eco47I	GGWCC	1 cut(s)	1116
Eco47III	AGCGCT	1 cut(s)	737
Eco53kI	GAGCTC	1 cut(s)	440
Eco57I	CTGAAG	2 cut(s)	45, 218
Eco88I	CYCGRG	1 cut(s)	1422
EcoICRI	GAGCTC	1 cut(s)	440
EcoRI	GAATTC	1 cut(s)	927
EcoRII	CCWGG	2 cut(s)	518, 817
EcoT14I	CCWWGG	2 cut(s)	539, 1286
EcoT22I	ATGCAT	2 cut(s)	1066, 1373
EcoT38I	GRGCYC	2 cut(s)	442, 1199
ErhI	CCWWGG	2 cut(s)	539, 1286
FaeI	CATG	6 cut(s)	248, 827, 1022, 1051, 1375, 1605
FalI	AAGNNNNNCTT	4 cut(s)	104, 136, 870, 902
FaqI	GGGAC	4 cut(s)	403, 800, 1102, 1268
FatI	CATG	6 cut(s)	244, 823, 1018, 1047, 1371, 1601
FauI	CCCGC	2 cut(s)	164, 438
FblI	GTMKAC	1 cut(s)	144
FokI	GGATG	2 cut(s)	365, 764
FriOI	GRGCYC	2 cut(s)	442, 1199
FspBI	CTAG	2 cut(s)	683, 1277
GlaI	GCGC	2 cut(s)	153, 737
GsaI	CCCAGC	1 cut(s)	206
HaeII	RGCGCY	2 cut(s)	155, 739
HaeIII	GGCC	5 cut(s)	100, 110, 862, 1088, 1420
HapII	CCGG	6 cut(s)	66, 107, 149, 189, 843, 1556
HgaI	GACGC	2 cut(s)	778, 819
HhaI	GCGC	2 cut(s)	154, 738
Hin1I	GRCGYC	1 cut(s)	789
Hin1II	CATG	6 cut(s)	248, 827, 1022, 1051, 1375, 1605
Hin6I	GCGC	2 cut(s)	152, 736
HinP1I	GCGC	2 cut(s)	152, 736
HincII	GTYRAC	3 cut(s)	76, 145, 769
HindII	GTYRAC	3 cut(s)	76, 145, 769
HindIII	AAGCTT	1 cut(s)	1346
HinfI	GANTC	1 cut(s)	80
HpaII	CCGG	6 cut(s)	66, 107, 149, 189, 843, 1556
HphI	GGTGA	4 cut(s)	109, 992, 1391, 1570
Hpy166II	GTNNAC	6 cut(s)	76, 117, 145, 474, 769, 1126
Hpy188I	TCNGA	6 cut(s)	87, 198, 933, 1212, 1235, 1399
Hpy8I	GTNNAC	6 cut(s)	76, 117, 145, 474, 769, 1126
Hpy99I	CGWCG	2 cut(s)	146, 415
HpyCH4III	ACNGT	5 cut(s)	242, 430, 536, 745, 1177
HpyCH4IV	ACGT	3 cut(s)	89, 657, 1537
HpyCH4V	TGCA	8 cut(s)	340, 755, 827, 876, 1064, 1183, 1371, 1439
HpyF10VI	GCNNNNNNNGC	7 cut(s)	177, 189, 446, 1039, 1433, 1436, 1452
HpyF3I	CTNAG	4 cut(s)	269, 305, 1198, 1338
HpySE526I	ACGT	3 cut(s)	89, 657, 1537
Hsp92I	GRCGYC	1 cut(s)	789
Hsp92II	CATG	6 cut(s)	248, 827, 1022, 1051, 1375, 1605
HspAI	GCGC	2 cut(s)	152, 736
KroI	GCCGGC	2 cut(s)	65, 106
KroNI	GCCGGC	2 cut(s)	67, 108
Kzo9I	GATC	6 cut(s)	211, 334, 414, 853, 1598, 1609
LmnI	GCTCC	7 cut(s)	191, 437, 716, 733, 1005, 1452, 1558
Lsp1109I	GCAGC	6 cut(s)	90, 839, 1420, 1423, 1439, 1442
LweI	GCATC	2 cut(s)	742, 1051
MaeI	CTAG	2 cut(s)	683, 1277
MaeII	ACGT	3 cut(s)	89, 657, 1537
MaeIII	GTNAC	3 cut(s)	55, 1294, 1558
MalI	GATC	6 cut(s)	213, 336, 416, 855, 1600, 1611
MbiI	CCGCTC	2 cut(s)	1042, 1553
MboI	GATC	6 cut(s)	211, 334, 414, 853, 1598, 1609
MboII	GAAGA	4 cut(s)	344, 391, 638, 1145
MfeI	CAATTG	2 cut(s)	591, 756
MflI	RGATCY	1 cut(s)	334
MhlI	GDGCHC	4 cut(s)	442, 842, 1010, 1199
MluCI	AATT	9 cut(s)	309, 591, 612, 756, 902, 927, 1111, 1323, 1413
MlyI	GAGTC	1 cut(s)	74
MmeI	TCCRAC	2 cut(s)	983, 1610
Mph1103I	ATGCAT	2 cut(s)	1066, 1373
MroNI	GCCGGC	2 cut(s)	65, 106
MseI	TTAA	6 cut(s)	297, 603, 639, 905, 1035, 1529
MslI	CAYNNNNRTG	1 cut(s)	1278
MspA1I	CMGCKG	2 cut(s)	103, 1433
MspI	CCGG	6 cut(s)	66, 107, 149, 189, 843, 1556
MspR9I	CCNGG	4 cut(s)	149, 520, 819, 844
MunI	CAATTG	2 cut(s)	591, 756
Mva1269I	GAATGC	1 cut(s)	192
MvaI	CCWGG	2 cut(s)	520, 819
MwoI	GCNNNNNNNGC	7 cut(s)	177, 189, 446, 1039, 1433, 1436, 1452
NaeI	GCCGGC	2 cut(s)	67, 108
NciI	CCSGG	2 cut(s)	149, 844
NdeII	GATC	6 cut(s)	211, 334, 414, 853, 1598, 1609
NgoMIV	GCCGGC	2 cut(s)	65, 106
NlaIII	CATG	6 cut(s)	248, 827, 1022, 1051, 1375, 1605
NlaIV	GGNNCC	4 cut(s)	187, 516, 841, 1118
NmeAIII	GCCGAG	1 cut(s)	1483
NmuCI	GTSAC	1 cut(s)	1558
NsiI	ATGCAT	2 cut(s)	1066, 1373
NspI	RCATGY	2 cut(s)	1022, 1375
NspV	TTCGAA	1 cut(s)	896
PaeR7I	CTCGAG	1 cut(s)	1422
PagI	TCATGA	1 cut(s)	244
PciI	ACATGT	1 cut(s)	1018
PcsI	WCGNNNNNNNCGW	1 cut(s)	419
PctI	GAATGC	1 cut(s)	192
PdiI	GCCGGC	2 cut(s)	67, 108
Ple19I	CGATCG	1 cut(s)	417
PleI	GAGTC	1 cut(s)	74
PpsI	GAGTC	1 cut(s)	74
PscI	ACATGT	1 cut(s)	1018
PshBI	ATTAAT	1 cut(s)	297
Psp124BI	GAGCTC	1 cut(s)	442
Psp6I	CCWGG	2 cut(s)	518, 817
PspFI	CCCAGC	1 cut(s)	202
PspGI	CCWGG	2 cut(s)	518, 817
PspN4I	GGNNCC	4 cut(s)	187, 516, 841, 1118
PspPI	GGNCC	2 cut(s)	1087, 1116
PspXI	VCTCGAGB	1 cut(s)	1422
PsrI	GAACNNNNNNTAC	2 cut(s)	704, 736
PstI	CTGCAG	1 cut(s)	1441
PstNI	CAGNNNCTG	2 cut(s)	1433, 1436
PsuI	RGATCY	1 cut(s)	334
PvuI	CGATCG	1 cut(s)	417
PvuII	CAGCTG	1 cut(s)	1433
RsaI	GTAC	5 cut(s)	218, 475, 501, 524, 690
RsaNI	GTAC	5 cut(s)	217, 474, 500, 523, 689
RseI	CAYNNNNRTG	1 cut(s)	1278
SacI	GAGCTC	1 cut(s)	442
SalI	GTCGAC	1 cut(s)	143
SaqAI	TTAA	6 cut(s)	297, 603, 639, 905, 1035, 1529
Sau3AI	GATC	6 cut(s)	211, 334, 414, 853, 1598, 1609
Sau96I	GGNCC	2 cut(s)	1087, 1116
SchI	GAGTC	1 cut(s)	74
ScrFI	CCNGG	4 cut(s)	149, 520, 819, 844
SduI	GDGCHC	4 cut(s)	442, 842, 1010, 1199
SfaNI	GCATC	2 cut(s)	742, 1051
SfcI	CTRYAG	1 cut(s)	1437
Sfr274I	CTCGAG	1 cut(s)	1422
SfuI	TTCGAA	1 cut(s)	896
SinI	GGWCC	1 cut(s)	1116
SlaI	CTCGAG	1 cut(s)	1422
SmiMI	CAYNNNNRTG	1 cut(s)	1278
SmlI	CTYRAG	1 cut(s)	1422
SmoI	CTYRAG	1 cut(s)	1422
Sse9I	AATT	9 cut(s)	309, 591, 612, 756, 902, 927, 1111, 1323, 1413
SsiI	CCGC	8 cut(s)	101, 171, 180, 183, 445, 978, 1042, 1551
SspMI	CTAG	2 cut(s)	683, 1277
SstI	GAGCTC	1 cut(s)	442
StyD4I	CCNGG	4 cut(s)	147, 518, 817, 842
StyI	CCWWGG	2 cut(s)	539, 1286
TaaI	ACNGT	5 cut(s)	242, 430, 536, 745, 1177
TaiI	ACGT	3 cut(s)	92, 660, 1540
TasI	AATT	9 cut(s)	309, 591, 612, 756, 902, 927, 1111, 1323, 1413
TatI	WGTACW	2 cut(s)	473, 499
TauI	GCSGC	4 cut(s)	103, 183, 186, 1553
Tru1I	TTAA	6 cut(s)	297, 603, 639, 905, 1035, 1529
Tru9I	TTAA	6 cut(s)	297, 603, 639, 905, 1035, 1529
TscAI	CASTG	3 cut(s)	273, 433, 1503
TseFI	GTSAC	1 cut(s)	1558
TseI	GCWGC	6 cut(s)	103, 827, 1427, 1430, 1433, 1436
Tsp45I	GTSAC	1 cut(s)	1558
TspDTI	ATGAA	3 cut(s)	638, 684, 999
TspGWI	ACGGA	3 cut(s)	130, 729, 1343
TspRI	CASTG	3 cut(s)	273, 433, 1503
VpaK11BI	GGWCC	1 cut(s)	1116
VspI	ATTAAT	1 cut(s)	297
XapI	RAATTY	1 cut(s)	927
XbaI	TCTAGA	2 cut(s)	682, 1276
XceI	RCATGY	2 cut(s)	1022, 1375
XhoI	CTCGAG	1 cut(s)	1422
XmiI	GTMKAC	1 cut(s)	144
XspI	CTAG	2 cut(s)	683, 1277
Zsp2I	ATGCAT	2 cut(s)	1066, 1373

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.