Rh1CG309300

Beta-hexosaminidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0013206
Physical Location & Seq
Forward (+)
58357680 .. 58363430
5751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG309300.1

Sequence Viewer

Length: 1611 bp
ATGGCGACTTCCTTTGTCTTGTTCCTATTCGTTTTCTCAACCGCGCTCTGTTTTTCTCAGGCCAAAGTCAAAGGCCCCATCACGTATTTATGGCCGTTGCCGGCGGAGTTCACTTCCGGCAACCACACGCTCACTGTCGACCCGGCGCTGTCGCTCGTCGTCGGCGGGAACGGGGGCAAGTCCGTCATTTTGAAGGAGGGGTTTGATCGGTACAAGGCGATTATATTCAAGAACAGTAATGGGGTTTCTGGGATTGATAGAATGAAGGCCAAGGGAGAAAGCTATGATATTAGTAAAGTCAGAGTTGTGGTGCAGTCGGCGAGCGAAGATCTTAATCTTGGTGTGGATGAGAGCTATACTCTGTTTGTGTTGAAGAAAGATGGGAAAGCTATTGTTGGGGAGGCGACTATTGAGGCAAACACAGTGTATGGTGCGTTGCGGGGGTTAGAGACTTTCAGCCAATTGTGTACTTTTGACTATGAAACTAAATCCATGCAAGTACATAAGTCACCATGGAACATACGAGACAAACCAAGGTTTGCATATCGAGGGCTTTTGCTTGATACATCAAGGCACTATTTACCAATTGACGTAATTAAGCAAGTAATTGAATCGATGTCATATGCTAAACTTAATGTTCTTCATTGGCACGTCATTGATAGAGAGTCATTTCCTCTAGAAGTACCTACATATCCCAAACTGTGGAAAGGTGCATATTCACAGTGGGAGAGATACACCGTTGAAGATGCATATGAAATTGTCAACTTTGCGAAAACTAGAGGCATCAATGTTATGGCAGAAGTAGATGTCCCTGGTCATGCAAAATCATGGGGCACTGGATATCCTGATCTCTGGCCTTCCTCTTCCTGCACAGAGCCTCTTGATGTTTCAAAGAATTTTACTTTTGACGTGCTTTCTGGCATTCTGACGGATTTGAGAAAGATTTTCCCCTTCGAGCTTTTCCACTTGGGTGGTGATGAAGTTAATACAACTTGCTGGGAAGCTACTCCACATGTAAAGAAATGGCTTAAGCAACAGGACATGACTGCCAAAGATGCCTACCAATATTTTGTTCTCAAAGCACAAGAAATAGCAATTTCCAAAAATTGGACCCCGGTCAACTGGGAAGAAACCTTCAACACCTTCCCTACGAAGCTCAATCCAAAGACTGTAGTGCATAACTGGTTGGGCGGAGGGGTTTGTCCAAAGGCTGTGGATAAAGGTTTCAGATGCATTTTCAGCAATCAAGGTGTTTGGTATCTTGACCATCTAGATGTCCCTTGGGATGCTACATATAATGCTGACCCGCTTGAAGGAATAGATGATTCTTCCAAGCAAAAGCTTGTCCTTGGAGGAGAAGTATGCATGTGGGGTGAGACTGCTGATCCATCAAATGTTCAGCAAACAATATGGCCTCGAGCTGCAGCAGCTGCAGAGCGCCTGTGGAGCAGAAAGGAGTCAACTTCTGGAAAAAGTAGTAATGAAACTGCGCTACCTCGGCTACACTACTTCAGATGCCTCTTGAATAGGCGTGGAGTTCAAGCCGCTCCAGTTGAAAACTTCTACGCTCGAAGTGCTCCTCCTGGTGCTGGCTCATGTTATGCTCAATAA

Protein Analysis

536

Amino Acids

60.15

Weight (kDa)

6.84

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycohydro_20b2 PF14845 30 - 155 9.1e-24 beta-acetyl hexosaminidase like
Glyco_hydro_20 PF00728 180 - 484 2.1e-82 Glycosyl hydrolase family 20, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013206)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65590 AT3G55260
fragaria_vesca FvH4_7g21240
malus_domestica MD01G1120700.v1.1 MD07G1190100.v1.1
prunus_persica Prupe.2G227600_v2.0.a1 Prupe.2G227600_v2.0.a1 Prupe.2G228000_v2.0.a1
pyrus_communis pycom01g14730 pycom07g17970
rosa_chinensis RchiOBHm_Chr1g0365681
rosa_laevigata RLG00000027418
rosa_roxburghii Rroxscaffold_4G00291090
rosa_samantha Rh1AG331300 Rh1BG292800 Rh1CG309300 Rh1DG324300
rosa_wichuraiana Rw1G029430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 702, 1107
AccBSI CCGCTC 1 cut(s) 1547
AccI GTMKAC 1 cut(s) 138
AccII CGCG 1 cut(s) 44
AciI CCGC 7 cut(s) 42, 104, 165, 439, 1191, 1307, 1545
AclWI GGATC 1 cut(s) 1379
AcoI YGGCCR 1 cut(s) 92
AcsI RAATTY 1 cut(s) 895
AcuI CTGAAG 1 cut(s) 1495
AfaI GTAC 4 cut(s) 212, 469, 501, 684
AfiI CCNNNNNNNGG 4 cut(s) 702, 1107, 1313, 1589
AflII CTTAAG 1 cut(s) 1028
AflIII ACRYGT 1 cut(s) 1012
AjiI CACGTC 2 cut(s) 652, 910
AjnI CCWGG 2 cut(s) 811, 1582
AjuI GAANNNNNNNTTGG 2 cut(s) 1056, 1088
AleI CACNNNNGTG 1 cut(s) 969
AluBI AGCT 9 cut(s) 282, 354, 389, 958, 1004, 1156, 1342, 1421, 1430
AluI AGCT 9 cut(s) 282, 354, 389, 958, 1004, 1156, 1342, 1421, 1430
Alw21I GWGCWC 1 cut(s) 1579
Alw26I GTCTC 3 cut(s) 443, 519, 1370
AlwI GGATC 1 cut(s) 1379
AlwNI CAGNNNCTG 1 cut(s) 1430
Ama87I CYCGRG 1 cut(s) 1416
AoxI GGCC 6 cut(s) 60, 73, 92, 267, 854, 1412
ApeKI GCWGC 4 cut(s) 1421, 1424, 1427, 1430
ApoI RAATTY 1 cut(s) 895
AspLEI GCGC 4 cut(s) 46, 148, 1440, 1492
AspS9I GGNCC 2 cut(s) 74, 1110
AsuC2I CCSGG 2 cut(s) 143, 1115
AsuHPI GGTGA 3 cut(s) 501, 986, 1385
AvaI CYCGRG 1 cut(s) 1416
AvaII GGWCC 1 cut(s) 1110
BaeGI GKGCMC 1 cut(s) 836
Bbv12I GWGCWC 1 cut(s) 1579
BbvI GCAGC 4 cut(s) 1408, 1417, 1436, 1439
BccI CCATC 4 cut(s) 86, 374, 1275, 1396
BceAI ACGGC 1 cut(s) 79
BciT130I CCWGG 2 cut(s) 813, 1584
BcnI CCSGG 2 cut(s) 143, 1115
BcoDI GTCTC 3 cut(s) 443, 519, 1370
BfaI CTAG 3 cut(s) 677, 777, 1271
BfmI CTRYAG 3 cut(s) 1170, 1422, 1431
BfoI RGCGCY 2 cut(s) 149, 1441
BfrI CTTAAG 1 cut(s) 1028
BglII AGATCT 1 cut(s) 328
BisI GCNGC 5 cut(s) 1422, 1425, 1428, 1431, 1545
BlsI GCNGC 5 cut(s) 1423, 1426, 1429, 1432, 1546
Bme1390I CCNGG 4 cut(s) 143, 813, 1115, 1584
Bme18I GGWCC 1 cut(s) 1110
BmeT110I CYCGRG 1 cut(s) 1416
BmgBI CACGTC 2 cut(s) 652, 910
BmgT120I GGNCC 2 cut(s) 74, 1110
BmiI GGNNCC 2 cut(s) 76, 1112
BmrFI CCNGG 4 cut(s) 143, 813, 1115, 1584
BmrI ACTGGG 1 cut(s) 1132
BmsI GCATC 6 cut(s) 736, 792, 1045, 1220, 1276, 1505
BmuI ACTGGG 1 cut(s) 1132
BoxI GACNNNNGTC 1 cut(s) 1115
BplI GAGNNNNNCTC 2 cut(s) 343, 375
BpmI CTGGAG 1 cut(s) 1533
BpuMI CCSGG 2 cut(s) 143, 1115
Bsa29I ATCGAT 1 cut(s) 614
BsaAI YACGTR 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 333
BsaJI CCNNGG 8 cut(s) 270, 512, 533, 811, 1113, 1280, 1348, 1496
BsaXI ACNNNNNCTCC 2 cut(s) 1344, 1374
Bsc4I CCNNNNNNNGG 4 cut(s) 702, 1107, 1313, 1589
Bse118I RCCGGY 1 cut(s) 100
Bse1I ACTGG 4 cut(s) 841, 1127, 1187, 1550
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 2 cut(s) 813, 1584
BseCI ATCGAT 1 cut(s) 614
BseDI CCNNGG 8 cut(s) 270, 512, 533, 811, 1113, 1280, 1348, 1496
BseGI GGATG 2 cut(s) 352, 1291
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 4 cut(s) 702, 1107, 1313, 1589
BseMII CTCAG 1 cut(s) 71
BseNI ACTGG 4 cut(s) 841, 1127, 1187, 1550
BseRI GAGGAG 2 cut(s) 1368, 1569
BseSI GKGCMC 1 cut(s) 836
BseXI GCAGC 4 cut(s) 1408, 1417, 1436, 1439
BseYI CCCAGC 1 cut(s) 996
BsgI GTGCAG 2 cut(s) 332, 853
Bsh1236I CGCG 1 cut(s) 44
BshFI GGCC 6 cut(s) 62, 75, 94, 269, 856, 1414
BshVI ATCGAT 1 cut(s) 614
BsiHKAI GWGCWC 1 cut(s) 1579
BsiHKCI CYCGRG 1 cut(s) 1416
BsiSI CCGG 4 cut(s) 101, 117, 143, 1115
BslFI GGGAC 2 cut(s) 794, 1262
BslI CCNNNNNNNGG 4 cut(s) 702, 1107, 1313, 1589
BsmAI GTCTC 3 cut(s) 443, 519, 1370
BsmFI GGGAC 2 cut(s) 794, 1262
BsmI GAATGC 1 cut(s) 921
BsnI GGCC 6 cut(s) 62, 75, 94, 269, 856, 1414
BsoBI CYCGRG 1 cut(s) 1416
Bsp1286I GDGCHC 2 cut(s) 836, 1579
Bsp143I GATC 4 cut(s) 205, 328, 847, 1384
Bsp19I CCATGG 1 cut(s) 512
BspACI CCGC 7 cut(s) 42, 104, 165, 439, 1191, 1307, 1545
BspANI GGCC 6 cut(s) 62, 75, 94, 269, 856, 1414
BspCNI CTCAG 1 cut(s) 70
BspDI ATCGAT 1 cut(s) 614
BspFNI CGCG 1 cut(s) 44
BspLI GGNNCC 2 cut(s) 76, 1112
BspMAI CTGCAG 2 cut(s) 1426, 1435
BspPI GGATC 1 cut(s) 1379
BspTI CTTAAG 1 cut(s) 1028
BsrBI CCGCTC 1 cut(s) 1547
BsrFI RCCGGY 1 cut(s) 100
BsrI ACTGG 4 cut(s) 841, 1127, 1187, 1550
BssAI RCCGGY 1 cut(s) 100
BssECI CCNNGG 8 cut(s) 270, 512, 533, 811, 1113, 1280, 1348, 1496
BssMI GATC 4 cut(s) 205, 328, 847, 1384
BssT1I CCWWGG 5 cut(s) 270, 512, 533, 1280, 1348
Bst2UI CCWGG 2 cut(s) 813, 1584
Bst4CI ACNGT 7 cut(s) 136, 236, 424, 702, 723, 739, 1171
Bst6I CTCTTC 1 cut(s) 868
BstAFI CTTAAG 1 cut(s) 1028
BstAPI GCANNNNNTGC 1 cut(s) 1430
BstBAI YACGTR 1 cut(s) 84
BstC8I GCNNGC 3 cut(s) 102, 322, 1591
BstDEI CTNAG 1 cut(s) 57
BstDSI CCRYGG 1 cut(s) 512
BstF5I GGATG 2 cut(s) 352, 1291
BstFNI CGCG 1 cut(s) 44
BstH2I RGCGCY 2 cut(s) 149, 1441
BstHHI GCGC 4 cut(s) 46, 148, 1440, 1492
BstKTI GATC 4 cut(s) 208, 331, 850, 1387
BstMAI GTCTC 3 cut(s) 443, 519, 1370
BstMBI GATC 4 cut(s) 205, 328, 847, 1384
BstMWI GCNNNNNNNGC 7 cut(s) 1055, 1239, 1427, 1430, 1446, 1498, 1574
BstNI CCWGG 2 cut(s) 813, 1584
BstNSI RCATGY 2 cut(s) 1016, 1369
BstPAI GACNNNNGTC 1 cut(s) 1115
BstSCI CCNGG 4 cut(s) 141, 811, 1113, 1582
BstSFI CTRYAG 3 cut(s) 1170, 1422, 1431
BstSLI GKGCMC 1 cut(s) 836
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 4 cut(s) 1408, 1417, 1436, 1439
BstX2I RGATCY 1 cut(s) 328
BstXI CCANNNNNNTGG 1 cut(s) 971
BstYI RGATCY 1 cut(s) 328
Bsu15I ATCGAT 1 cut(s) 614
BsuRI GGCC 6 cut(s) 62, 75, 94, 269, 856, 1414
BsuTUI ATCGAT 1 cut(s) 614
BtgI CCRYGG 1 cut(s) 512
BtrI CACGTC 2 cut(s) 652, 910
BtsCI GGATG 2 cut(s) 352, 1291
BtsIMutI CAGTG 4 cut(s) 132, 429, 728, 834
Cac8I GCNNGC 3 cut(s) 102, 322, 1591
CaiI CAGNNNCTG 1 cut(s) 1430
CfoI GCGC 4 cut(s) 46, 148, 1440, 1492
Cfr10I RCCGGY 1 cut(s) 100
Cfr13I GGNCC 2 cut(s) 74, 1110
ClaI ATCGAT 1 cut(s) 614
Csp6I GTAC 4 cut(s) 211, 468, 500, 683
CspCI CAANNNNNGTGG 2 cut(s) 1194, 1229
CviAII CATG 8 cut(s) 493, 513, 818, 828, 1013, 1042, 1366, 1596
CviQI GTAC 4 cut(s) 211, 468, 500, 683
DdeI CTNAG 1 cut(s) 57
DpnI GATC 4 cut(s) 207, 330, 849, 1386
DpnII GATC 4 cut(s) 205, 328, 847, 1384
EaeI YGGCCR 1 cut(s) 92
Eam1104I CTCTTC 1 cut(s) 868
EarI CTCTTC 1 cut(s) 868
EciI GGCGGA 2 cut(s) 119, 1206
Eco130I CCWWGG 5 cut(s) 270, 512, 533, 1280, 1348
Eco32I GATATC 1 cut(s) 842
Eco47I GGWCC 1 cut(s) 1110
Eco57I CTGAAG 1 cut(s) 1495
Eco88I CYCGRG 1 cut(s) 1416
EcoO109I RGGNCCY 1 cut(s) 74
EcoRII CCWGG 2 cut(s) 811, 1582
EcoRV GATATC 1 cut(s) 842
EcoT14I CCWWGG 5 cut(s) 270, 512, 533, 1280, 1348
EcoT22I ATGCAT 3 cut(s) 751, 1235, 1367
ErhI CCWWGG 5 cut(s) 270, 512, 533, 1280, 1348
FaeI CATG 8 cut(s) 496, 516, 821, 831, 1016, 1045, 1369, 1599
FaqI GGGAC 2 cut(s) 794, 1262
FatI CATG 8 cut(s) 492, 512, 817, 827, 1012, 1041, 1365, 1595
FauI CCCGC 3 cut(s) 158, 432, 1314
FauNDI CATATG 2 cut(s) 622, 751
FblI GTMKAC 1 cut(s) 138
Fnu4HI GCNGC 5 cut(s) 1422, 1425, 1428, 1431, 1545
FokI GGATG 2 cut(s) 359, 1298
Fsp4HI GCNGC 5 cut(s) 1422, 1425, 1428, 1431, 1545
FspBI CTAG 3 cut(s) 677, 777, 1271
GlaI GCGC 4 cut(s) 45, 147, 1439, 1491
GluI GCNGC 5 cut(s) 1422, 1425, 1428, 1431, 1545
GsaI CCCAGC 1 cut(s) 1000
GsuI CTGGAG 1 cut(s) 1533
HaeII RGCGCY 2 cut(s) 149, 1441
HaeIII GGCC 6 cut(s) 62, 75, 94, 269, 856, 1414
HapII CCGG 4 cut(s) 101, 117, 143, 1115
HhaI GCGC 4 cut(s) 46, 148, 1440, 1492
Hin1II CATG 8 cut(s) 496, 516, 821, 831, 1016, 1045, 1369, 1599
Hin6I GCGC 4 cut(s) 44, 146, 1438, 1490
HinP1I GCGC 4 cut(s) 44, 146, 1438, 1490
HincII GTYRAC 4 cut(s) 139, 763, 1120, 1461
HindII GTYRAC 4 cut(s) 139, 763, 1120, 1461
HindIII AAGCTT 1 cut(s) 1340
HinfI GANTC 4 cut(s) 611, 665, 1325, 1457
HpaII CCGG 4 cut(s) 101, 117, 143, 1115
HphI GGTGA 3 cut(s) 501, 986, 1385
Hpy166II GTNNAC 6 cut(s) 111, 139, 468, 763, 1120, 1461
Hpy188I TCNGA 4 cut(s) 302, 927, 1229, 1514
Hpy188III TCNNGA 8 cut(s) 229, 677, 845, 881, 1262, 1271, 1467, 1522
Hpy8I GTNNAC 6 cut(s) 111, 139, 468, 763, 1120, 1461
Hpy99I CGWCG 2 cut(s) 161, 164
HpyAV CCTTC 7 cut(s) 187, 259, 867, 961, 1144, 1153, 1307
HpyCH4III ACNGT 7 cut(s) 136, 236, 424, 702, 723, 739, 1171
HpyCH4IV ACGT 4 cut(s) 83, 591, 651, 909
HpyF10VI GCNNNNNNNGC 7 cut(s) 1055, 1239, 1427, 1430, 1446, 1498, 1574
HpyF3I CTNAG 1 cut(s) 57
HpySE526I ACGT 4 cut(s) 83, 591, 651, 909
Hsp92II CATG 8 cut(s) 496, 516, 821, 831, 1016, 1045, 1369, 1599
HspAI GCGC 4 cut(s) 44, 146, 1438, 1490
KroI GCCGGC 1 cut(s) 100
KroNI GCCGGC 1 cut(s) 102
Kzo9I GATC 4 cut(s) 205, 328, 847, 1384
LmnI GCTCC 3 cut(s) 1446, 1552, 1582
Lsp1109I GCAGC 4 cut(s) 1408, 1417, 1436, 1439
LweI GCATC 6 cut(s) 736, 792, 1045, 1220, 1276, 1505
MaeI CTAG 3 cut(s) 677, 777, 1271
MaeII ACGT 4 cut(s) 83, 591, 651, 909
MaeIII GTNAC 1 cut(s) 507
MalI GATC 4 cut(s) 207, 330, 849, 1386
MbiI CCGCTC 1 cut(s) 1547
MboI GATC 4 cut(s) 205, 328, 847, 1384
MboII GAAGA 7 cut(s) 338, 385, 632, 755, 855, 1139, 1320
MfeI CAATTG 2 cut(s) 461, 585
MflI RGATCY 1 cut(s) 328
MhlI GDGCHC 2 cut(s) 836, 1579
MluCI AATT 8 cut(s) 461, 585, 594, 606, 756, 895, 1095, 1105
MlyI GAGTC 2 cut(s) 674, 1466
Mph1103I ATGCAT 3 cut(s) 751, 1235, 1367
MroNI GCCGGC 1 cut(s) 100
MseI TTAA 5 cut(s) 333, 597, 633, 984, 1029
MslI CAYNNNNRTG 2 cut(s) 969, 1272
MspA1I CMGCKG 1 cut(s) 1430
MspCI CTTAAG 1 cut(s) 1028
MspI CCGG 4 cut(s) 101, 117, 143, 1115
MspR9I CCNGG 4 cut(s) 143, 813, 1115, 1584
MunI CAATTG 2 cut(s) 461, 585
Mva1269I GAATGC 1 cut(s) 921
MvaI CCWGG 2 cut(s) 813, 1584
MvnI CGCG 1 cut(s) 44
MwoI GCNNNNNNNGC 7 cut(s) 1055, 1239, 1427, 1430, 1446, 1498, 1574
NaeI GCCGGC 1 cut(s) 102
NciI CCSGG 2 cut(s) 143, 1115
NcoI CCATGG 1 cut(s) 512
NdeI CATATG 2 cut(s) 622, 751
NdeII GATC 4 cut(s) 205, 328, 847, 1384
NgoMIV GCCGGC 1 cut(s) 100
NlaIII CATG 8 cut(s) 496, 516, 821, 831, 1016, 1045, 1369, 1599
NlaIV GGNNCC 2 cut(s) 76, 1112
NmeAIII GCCGAG 1 cut(s) 1477
NmuCI GTSAC 1 cut(s) 507
NsiI ATGCAT 3 cut(s) 751, 1235, 1367
NspI RCATGY 2 cut(s) 1016, 1369
OliI CACNNNNGTG 1 cut(s) 969
PaeR7I CTCGAG 1 cut(s) 1416
PciI ACATGT 1 cut(s) 1012
PctI GAATGC 1 cut(s) 921
PdiI GCCGGC 1 cut(s) 102
PfeI GAWTC 2 cut(s) 611, 1325
PflMI CCANNNNNTGG 2 cut(s) 702, 1107
PkrI GCNGC 5 cut(s) 1423, 1426, 1429, 1432, 1546
PleI GAGTC 2 cut(s) 673, 1465
PpsI GAGTC 2 cut(s) 673, 1465
Ppu21I YACGTR 1 cut(s) 84
PscI ACATGT 1 cut(s) 1012
PshAI GACNNNNGTC 1 cut(s) 1115
Psp6I CCWGG 2 cut(s) 811, 1582
PspFI CCCAGC 1 cut(s) 996
PspGI CCWGG 2 cut(s) 811, 1582
PspN4I GGNNCC 2 cut(s) 76, 1112
PspPI GGNCC 2 cut(s) 74, 1110
PspXI VCTCGAGB 1 cut(s) 1416
PstI CTGCAG 2 cut(s) 1426, 1435
PstNI CAGNNNCTG 1 cut(s) 1430
PsuI RGATCY 1 cut(s) 328
PvuII CAGCTG 1 cut(s) 1430
RsaI GTAC 4 cut(s) 212, 469, 501, 684
RsaNI GTAC 4 cut(s) 211, 468, 500, 683
RseI CAYNNNNRTG 2 cut(s) 969, 1272
SalI GTCGAC 1 cut(s) 137
SaqAI TTAA 5 cut(s) 333, 597, 633, 984, 1029
SatI GCNGC 5 cut(s) 1422, 1425, 1428, 1431, 1545
Sau3AI GATC 4 cut(s) 205, 328, 847, 1384
Sau96I GGNCC 2 cut(s) 74, 1110
SchI GAGTC 2 cut(s) 674, 1466
ScrFI CCNGG 4 cut(s) 143, 813, 1115, 1584
SduI GDGCHC 2 cut(s) 836, 1579
SfaNI GCATC 6 cut(s) 736, 792, 1045, 1220, 1276, 1505
SfcI CTRYAG 3 cut(s) 1170, 1422, 1431
Sfr274I CTCGAG 1 cut(s) 1416
SinI GGWCC 1 cut(s) 1110
SlaI CTCGAG 1 cut(s) 1416
SmiMI CAYNNNNRTG 2 cut(s) 969, 1272
SmlI CTYRAG 2 cut(s) 1028, 1416
SmoI CTYRAG 2 cut(s) 1028, 1416
Sse9I AATT 8 cut(s) 461, 585, 594, 606, 756, 895, 1095, 1105
SsiI CCGC 7 cut(s) 42, 104, 165, 439, 1191, 1307, 1545
SspI AATATT 1 cut(s) 1067
SspMI CTAG 3 cut(s) 677, 777, 1271
StyD4I CCNGG 4 cut(s) 141, 811, 1113, 1582
StyI CCWWGG 5 cut(s) 270, 512, 533, 1280, 1348
TaaI ACNGT 7 cut(s) 136, 236, 424, 702, 723, 739, 1171
TaiI ACGT 4 cut(s) 86, 594, 654, 912
TaqI TCGA 6 cut(s) 138, 547, 614, 954, 1417, 1570
TasI AATT 8 cut(s) 461, 585, 594, 606, 756, 895, 1095, 1105
TatI WGTACW 2 cut(s) 467, 499
TauI GCSGC 1 cut(s) 1547
TfiI GAWTC 2 cut(s) 611, 1325
Tru1I TTAA 5 cut(s) 333, 597, 633, 984, 1029
Tru9I TTAA 5 cut(s) 333, 597, 633, 984, 1029
TscAI CASTG 4 cut(s) 139, 429, 728, 841
TseFI GTSAC 1 cut(s) 507
TseI GCWGC 4 cut(s) 1421, 1424, 1427, 1430
Tsp45I GTSAC 1 cut(s) 507
TspDTI ATGAA 6 cut(s) 278, 495, 632, 768, 993, 1497
TspGWI ACGGA 2 cut(s) 172, 944
TspRI CASTG 4 cut(s) 139, 429, 728, 841
Van91I CCANNNNNTGG 2 cut(s) 702, 1107
Vha464I CTTAAG 1 cut(s) 1028
VpaK11BI GGWCC 1 cut(s) 1110
XapI RAATTY 1 cut(s) 895
XbaI TCTAGA 2 cut(s) 676, 1270
XceI RCATGY 2 cut(s) 1016, 1369
XhoI CTCGAG 1 cut(s) 1416
XmiI GTMKAC 1 cut(s) 138
XspI CTAG 3 cut(s) 677, 777, 1271
Zsp2I ATGCAT 3 cut(s) 751, 1235, 1367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.