Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0013206

Physical Location & Seq

Genomic Coordinates Reverse (-)

11358957 .. 11366383

Gene Structure 7427 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027418

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGGCGACTTCCCTTATCTTGTTCCTATTCGTTTTCTCGAACGCGCTCTGTTTTTCTCAGGCCAAAGTCAGAGGGCCCATCACGTATTTATGGCCGTTGCCGGCGGAGTTTTCTTCCGGCAACCACACGCTCACAGTCGACCCGGCGCTGTCGCTCGTCGTCGGCGGGAACGGGGGCAAGTCCGTCATTTTGAAGGAGGGGTTTGATCGGTACAAGGCGATTATATTCAAGAACAGTTATGGGGTTACCGGGATTGATAGAATGAAGGCCAAGGGAGAGAGCTATGATATTAGTAAAGTCAGAGTTGTGGTGCAGTCGGAGAGCGAAGATCTTAATCTTGGTGTGGATGAGAGCTATACTCTGTTTGTGTTGAAGGAAGATGGGAAAGCTATTGTTGGGGAGGCCACTATTGAGGCAAACACAGTGTATGGTGCGTTGCGGGGGTTAGAGACTTTCAGTCAATTGTGTACTTTTGACTATGAAACTAAATCCATGCAAGTACATAAGTCACCATGGAACATACGAGACAAACCAAGGTTCGCATATCGAGGGCTTTTGCTTGATACATCAAGGCACTATTTACCAATTGACGTAATTAAGCAAGTAATTGAATCGATGTCATATGCTAAACTTAATGTTCTTCATTGGCACGTCATTGATAGAGAGTCATTTCCTCTAGAAGTACCTACATATCCCAAATTGTGGAAAGGTGCATATTCACAGTGGGAGAGATACACCATTGAAGATGCATATGAAATTGTCAACTTTGCGAAGACTAGAGGCATCAATGTTATGGCAGAAGTAGATGTCCCTGGTCATGCACAATCATGGGGCACTGGATATCCTGATCTCTGGCCTTCCTCTTCCTGCAAAGAGCCTCTTGATGTTTCAAAGAATTTTACTTTCGACGTGCTTTCTGGCATTCTGACAGATTTGAGAAAGATTTTCCCCTTCGAGCTTTTCCACTTGGGTGGTGATGAAGTTAATACAACTTGCTGGGAAGTTACTCCACATGTAAAGAAATGGCTTAAGCAACAGGACAAGACTGCCAAAGACGCCTATCAATATTTTGTTCTCAAAGCACAAGAAATAGCAATTTCCAAAAATTGGACCCCGGTCAACTGGGAAGAAACCTTCAACACTTTCCCAACGAAGCTCAATCCAAAGACTGTAGTGCATAACTGGTTGGGCGGAGGGGTTTGTCCAAAGGCTGTTGATCAAGGTTTCAGATGTATTTTCAGCAATCAAGGTGTTTGGTATCTTGACCATCTAGATGTCCCTTGGGATGCTACATATAATGCCGACCCACTTGAAGGAATAGATGATTCTTCCAAGCAAAAGCTTGTCCTTGGAGGAGAAGTATGCATGTGGGCTGAGACTGCTGATCCATCAAATGTTCAGCAAACAATATGGCCTCGAGCTGCAGCAGCTGCAGAGCGCCTGTGGAGCACAAAGCAGTCAACTTCTGGAAAAAGTAGTAATGAAACTGCGTTACCTCGGCTACACTACTTCAGATGCCTCTTGAATAGGCGTGGAGTTCAAGCCGCTCCAGTTGAAAACTCCTACGCTCGAAGTGCTCCACCTGTCGGATCCGGGTTCGGGTCGAAAGTGGATCCGTCACACTCTCTGAAACAGTCAAATAAGAAAAGGAAGCAATTGAAACCCAGTGGCTTACTCCATTCTTCTTCTTCTTCCCTCCTCTGCTTCGCTTCGTCTTCTTCTCTCTCTAAAACCCCTGAAGCTTTTCACAGTCACAGCTCTGCTCTGGGTCTCGCACACTTAGGGTTTCTGGTGTCTCAATCTTGTGTTTGTAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004563 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005829 GO:0009505 GO:0015929 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00511 ko00513 ko00520 ko00531 ko00603 ko00604 ko01100 ko04142 map00511 map00513 map00520 map00531 map00603 map00604 map01100 map04142

Pfam Domains

Protein Families

Glyco_hydro_20 Glycohydro_20b2

Protein Analysis

606

Amino Acids

67.46

Weight (kDa)

7.9

Isoelectric Point (pI)

41.92

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glycohydro_20b2	PF14845	30 - 155	1.1e-23	beta-acetyl hexosaminidase like
Glyco_hydro_20	PF00728	180 - 484	1.6e-82	Glycosyl hydrolase family 20, catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013206)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65590 AT3G55260
fragaria_vesca	FvH4_7g21240
malus_domestica	MD01G1120700.v1.1 MD07G1190100.v1.1
prunus_persica	Prupe.2G227600_v2.0.a1 Prupe.2G227600_v2.0.a1 Prupe.2G228000_v2.0.a1
pyrus_communis	pycom01g14730 pycom07g17970
rosa_chinensis	RchiOBHm_Chr1g0365681
rosa_laevigata	RLG00000027418
rosa_roxburghii	Rroxscaffold_4G00291090
rosa_samantha	Rh1AG331300 Rh1BG292800 Rh1CG309300 Rh1DG324300
rosa_wichuraiana	Rw1G029430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	702, 1107
AccBSI	CCGCTC	1 cut(s)	1547
AccI	GTMKAC	1 cut(s)	138
AccII	CGCG	1 cut(s)	44
AciI	CCGC	5 cut(s)	104, 165, 439, 1191, 1545
AclWI	GGATC	5 cut(s)	1379, 1584, 1597, 1607, 1620
AcoI	YGGCCR	1 cut(s)	92
AcsI	RAATTY	1 cut(s)	895
AcuI	CTGAAG	2 cut(s)	1495, 1758
AcyI	GRCGYC	1 cut(s)	1056
AfaI	GTAC	4 cut(s)	212, 469, 501, 684
AfiI	CCNNNNNNNGG	5 cut(s)	702, 1107, 1313, 1586, 1599
AflII	CTTAAG	1 cut(s)	1028
AflIII	ACRYGT	1 cut(s)	1012
AhdI	GACNNNNNGTC	1 cut(s)	456
AjiI	CACGTC	2 cut(s)	652, 910
AjnI	CCWGG	1 cut(s)	811
AleI	CACNNNNGTG	1 cut(s)	969
Alw21I	GWGCWC	2 cut(s)	1451, 1579
Alw26I	GTCTC	5 cut(s)	443, 519, 1370, 1775, 1800
AlwI	GGATC	5 cut(s)	1379, 1584, 1597, 1607, 1620
AlwNI	CAGNNNCTG	1 cut(s)	1430
Ama87I	CYCGRG	1 cut(s)	1416
AoxI	GGCC	7 cut(s)	60, 74, 92, 267, 402, 854, 1412
ApaI	GGGCCC	1 cut(s)	78
ApeKI	GCWGC	4 cut(s)	1421, 1424, 1427, 1430
ApoI	RAATTY	1 cut(s)	895
Asp700I	GAANNNNTTC	1 cut(s)	1743
AspLEI	GCGC	3 cut(s)	46, 148, 1440
AspS9I	GGNCC	3 cut(s)	74, 75, 1110
AsuC2I	CCSGG	4 cut(s)	143, 250, 1115, 1594
AsuHPI	GGTGA	2 cut(s)	501, 986
AvaI	CYCGRG	1 cut(s)	1416
AvaII	GGWCC	1 cut(s)	1110
BaeGI	GKGCMC	2 cut(s)	78, 836
BamHI	GGATCC	2 cut(s)	1589, 1612
BanII	GRGCYC	1 cut(s)	78
BbsI	GAAGAC	2 cut(s)	779, 1707
Bbv12I	GWGCWC	2 cut(s)	1451, 1579
BbvI	GCAGC	4 cut(s)	1408, 1417, 1436, 1439
BccI	CCATC	4 cut(s)	86, 374, 1275, 1396
BceAI	ACGGC	1 cut(s)	79
BciT130I	CCWGG	1 cut(s)	813
BclI	TGATCA	1 cut(s)	1216
BcnI	CCSGG	4 cut(s)	143, 250, 1115, 1594
BcoDI	GTCTC	5 cut(s)	443, 519, 1370, 1775, 1800
BfaI	CTAG	3 cut(s)	677, 777, 1271
BfmI	CTRYAG	3 cut(s)	1170, 1422, 1431
BfoI	RGCGCY	2 cut(s)	149, 1441
BfrI	CTTAAG	1 cut(s)	1028
BglII	AGATCT	1 cut(s)	328
BisI	GCNGC	5 cut(s)	1422, 1425, 1428, 1431, 1545
BlsI	GCNGC	5 cut(s)	1423, 1426, 1429, 1432, 1546
Bme1390I	CCNGG	5 cut(s)	143, 250, 813, 1115, 1594
Bme18I	GGWCC	1 cut(s)	1110
BmeRI	GACNNNNNGTC	1 cut(s)	456
BmeT110I	CYCGRG	1 cut(s)	1416
BmgBI	CACGTC	2 cut(s)	652, 910
BmgT120I	GGNCC	3 cut(s)	74, 75, 1110
BmiI	GGNNCC	4 cut(s)	76, 1112, 1591, 1614
BmrFI	CCNGG	5 cut(s)	143, 250, 813, 1115, 1594
BmrI	ACTGGG	2 cut(s)	1132, 1659
BmsI	GCATC	4 cut(s)	736, 792, 1276, 1505
BmuI	ACTGGG	2 cut(s)	1132, 1659
BoxI	GACNNNNGTC	1 cut(s)	1115
BpiI	GAAGAC	2 cut(s)	779, 1707
BplI	GAGNNNNNCTC	2 cut(s)	343, 375
BpmI	CTGGAG	1 cut(s)	1533
BpuMI	CCSGG	4 cut(s)	143, 250, 1115, 1594
Bsa29I	ATCGAT	1 cut(s)	614
BsaAI	YACGTR	1 cut(s)	84
BsaBI	GATNNNNATC	1 cut(s)	333
BsaHI	GRCGYC	1 cut(s)	1056
BsaI	GGTCTC	1 cut(s)	1775
BsaJI	CCNNGG	8 cut(s)	270, 512, 533, 811, 1113, 1280, 1348, 1496
BsaXI	ACNNNNNCTCC	2 cut(s)	1344, 1374
Bsc4I	CCNNNNNNNGG	5 cut(s)	702, 1107, 1313, 1586, 1599
Bse118I	RCCGGY	1 cut(s)	100
Bse1I	ACTGG	5 cut(s)	841, 1127, 1187, 1550, 1665
Bse8I	GATNNNNATC	1 cut(s)	333
BseBI	CCWGG	1 cut(s)	813
BseCI	ATCGAT	1 cut(s)	614
BseDI	CCNNGG	8 cut(s)	270, 512, 533, 811, 1113, 1280, 1348, 1496
BseGI	GGATG	2 cut(s)	352, 1291
BseJI	GATNNNNATC	1 cut(s)	333
BseLI	CCNNNNNNNGG	5 cut(s)	702, 1107, 1313, 1586, 1599
BseMII	CTCAG	2 cut(s)	71, 1365
BseNI	ACTGG	5 cut(s)	841, 1127, 1187, 1550, 1665
BseRI	GAGGAG	2 cut(s)	1368, 1688
BseSI	GKGCMC	2 cut(s)	78, 836
BseXI	GCAGC	4 cut(s)	1408, 1417, 1436, 1439
BseYI	CCCAGC	1 cut(s)	996
BsgI	GTGCAG	1 cut(s)	332
Bsh1236I	CGCG	1 cut(s)	44
BshFI	GGCC	7 cut(s)	62, 76, 94, 269, 404, 856, 1414
BshVI	ATCGAT	1 cut(s)	614
BsiHKAI	GWGCWC	2 cut(s)	1451, 1579
BsiHKCI	CYCGRG	1 cut(s)	1416
BsiSI	CCGG	6 cut(s)	101, 117, 143, 249, 1115, 1593
BslFI	GGGAC	2 cut(s)	794, 1262
BslI	CCNNNNNNNGG	5 cut(s)	702, 1107, 1313, 1586, 1599
BsmAI	GTCTC	5 cut(s)	443, 519, 1370, 1775, 1800
BsmFI	GGGAC	2 cut(s)	794, 1262
BsmI	GAATGC	1 cut(s)	921
BsnI	GGCC	7 cut(s)	62, 76, 94, 269, 404, 856, 1414
Bso31I	GGTCTC	1 cut(s)	1775
BsoBI	CYCGRG	1 cut(s)	1416
Bsp120I	GGGCCC	1 cut(s)	74
Bsp1286I	GDGCHC	4 cut(s)	78, 836, 1451, 1579
Bsp143I	GATC	7 cut(s)	205, 328, 847, 1216, 1384, 1589, 1612
Bsp19I	CCATGG	1 cut(s)	512
BspACI	CCGC	5 cut(s)	104, 165, 439, 1191, 1545
BspANI	GGCC	7 cut(s)	62, 76, 94, 269, 404, 856, 1414
BspCNI	CTCAG	2 cut(s)	70, 1366
BspDI	ATCGAT	1 cut(s)	614
BspFNI	CGCG	1 cut(s)	44
BspLI	GGNNCC	4 cut(s)	76, 1112, 1591, 1614
BspMAI	CTGCAG	2 cut(s)	1426, 1435
BspPI	GGATC	5 cut(s)	1379, 1584, 1597, 1607, 1620
BspTI	CTTAAG	1 cut(s)	1028
BspTNI	GGTCTC	1 cut(s)	1775
BsrBI	CCGCTC	1 cut(s)	1547
BsrFI	RCCGGY	1 cut(s)	100
BsrI	ACTGG	5 cut(s)	841, 1127, 1187, 1550, 1665
BssAI	RCCGGY	1 cut(s)	100
BssECI	CCNNGG	8 cut(s)	270, 512, 533, 811, 1113, 1280, 1348, 1496
BssMI	GATC	7 cut(s)	205, 328, 847, 1216, 1384, 1589, 1612
BssNI	GRCGYC	1 cut(s)	1056
BssT1I	CCWWGG	5 cut(s)	270, 512, 533, 1280, 1348
Bst2UI	CCWGG	1 cut(s)	813
Bst4CI	ACNGT	7 cut(s)	136, 236, 424, 723, 1171, 1635, 1751
Bst6I	CTCTTC	1 cut(s)	868
BstACI	GRCGYC	1 cut(s)	1056
BstAFI	CTTAAG	1 cut(s)	1028
BstAPI	GCANNNNNTGC	1 cut(s)	1430
BstBAI	YACGTR	1 cut(s)	84
BstC8I	GCNNGC	1 cut(s)	102
BstDEI	CTNAG	3 cut(s)	57, 1374, 1780
BstDSI	CCRYGG	1 cut(s)	512
BstEII	GGTNACC	1 cut(s)	244
BstF5I	GGATG	2 cut(s)	352, 1291
BstFNI	CGCG	1 cut(s)	44
BstH2I	RGCGCY	2 cut(s)	149, 1441
BstHHI	GCGC	3 cut(s)	46, 148, 1440
BstKTI	GATC	7 cut(s)	208, 331, 850, 1219, 1387, 1592, 1615
BstMAI	GTCTC	5 cut(s)	443, 519, 1370, 1775, 1800
BstMBI	GATC	7 cut(s)	205, 328, 847, 1216, 1384, 1589, 1612
BstMWI	GCNNNNNNNGC	6 cut(s)	1055, 1379, 1427, 1430, 1446, 1574
BstNI	CCWGG	1 cut(s)	813
BstNSI	RCATGY	2 cut(s)	1016, 1369
BstPAI	GACNNNNGTC	1 cut(s)	1115
BstPI	GGTNACC	1 cut(s)	244
BstSCI	CCNGG	5 cut(s)	141, 248, 811, 1113, 1592
BstSFI	CTRYAG	3 cut(s)	1170, 1422, 1431
BstSLI	GKGCMC	2 cut(s)	78, 836
BstUI	CGCG	1 cut(s)	44
BstV1I	GCAGC	4 cut(s)	1408, 1417, 1436, 1439
BstV2I	GAAGAC	2 cut(s)	779, 1707
BstX2I	RGATCY	3 cut(s)	328, 1589, 1612
BstXI	CCANNNNNNTGG	1 cut(s)	971
BstYI	RGATCY	3 cut(s)	328, 1589, 1612
Bsu15I	ATCGAT	1 cut(s)	614
BsuRI	GGCC	7 cut(s)	62, 76, 94, 269, 404, 856, 1414
BsuTUI	ATCGAT	1 cut(s)	614
BtgI	CCRYGG	1 cut(s)	512
BtrI	CACGTC	2 cut(s)	652, 910
BtsCI	GGATG	2 cut(s)	352, 1291
BtsIMutI	CAGTG	4 cut(s)	429, 728, 834, 1672
Cac8I	GCNNGC	1 cut(s)	102
CaiI	CAGNNNCTG	1 cut(s)	1430
CfoI	GCGC	3 cut(s)	46, 148, 1440
Cfr10I	RCCGGY	1 cut(s)	100
Cfr13I	GGNCC	3 cut(s)	74, 75, 1110
ClaI	ATCGAT	1 cut(s)	614
CseI	GACGC	1 cut(s)	1064
Csp6I	GTAC	4 cut(s)	211, 468, 500, 683
CviAII	CATG	6 cut(s)	493, 513, 818, 828, 1013, 1366
CviQI	GTAC	4 cut(s)	211, 468, 500, 683
DdeI	CTNAG	3 cut(s)	57, 1374, 1780
DpnI	GATC	7 cut(s)	207, 330, 849, 1218, 1386, 1591, 1614
DpnII	GATC	7 cut(s)	205, 328, 847, 1216, 1384, 1589, 1612
DriI	GACNNNNNGTC	1 cut(s)	456
EaeI	YGGCCR	1 cut(s)	92
Eam1104I	CTCTTC	1 cut(s)	868
Eam1105I	GACNNNNNGTC	1 cut(s)	456
EarI	CTCTTC	1 cut(s)	868
EciI	GGCGGA	2 cut(s)	119, 1206
Eco130I	CCWWGG	5 cut(s)	270, 512, 533, 1280, 1348
Eco24I	GRGCYC	1 cut(s)	78
Eco31I	GGTCTC	1 cut(s)	1775
Eco32I	GATATC	1 cut(s)	842
Eco47I	GGWCC	1 cut(s)	1110
Eco57I	CTGAAG	2 cut(s)	1495, 1758
Eco88I	CYCGRG	1 cut(s)	1416
Eco91I	GGTNACC	1 cut(s)	244
EcoO109I	RGGNCCY	1 cut(s)	74
EcoO65I	GGTNACC	1 cut(s)	244
EcoRII	CCWGG	1 cut(s)	811
EcoRV	GATATC	1 cut(s)	842
EcoT14I	CCWWGG	5 cut(s)	270, 512, 533, 1280, 1348
EcoT22I	ATGCAT	2 cut(s)	751, 1367
EcoT38I	GRGCYC	1 cut(s)	78
ErhI	CCWWGG	5 cut(s)	270, 512, 533, 1280, 1348
FaeI	CATG	6 cut(s)	496, 516, 821, 831, 1016, 1369
FalI	AAGNNNNNCTT	2 cut(s)	864, 896
FaqI	GGGAC	2 cut(s)	794, 1262
FatI	CATG	6 cut(s)	492, 512, 817, 827, 1012, 1365
FauI	CCCGC	2 cut(s)	158, 432
FauNDI	CATATG	2 cut(s)	622, 751
FbaI	TGATCA	1 cut(s)	1216
FblI	GTMKAC	1 cut(s)	138
Fnu4HI	GCNGC	5 cut(s)	1422, 1425, 1428, 1431, 1545
FokI	GGATG	2 cut(s)	359, 1298
FriOI	GRGCYC	1 cut(s)	78
Fsp4HI	GCNGC	5 cut(s)	1422, 1425, 1428, 1431, 1545
FspBI	CTAG	3 cut(s)	677, 777, 1271
GlaI	GCGC	3 cut(s)	45, 147, 1439
GluI	GCNGC	5 cut(s)	1422, 1425, 1428, 1431, 1545
GsaI	CCCAGC	1 cut(s)	1000
GsuI	CTGGAG	1 cut(s)	1533
HaeII	RGCGCY	2 cut(s)	149, 1441
HaeIII	GGCC	7 cut(s)	62, 76, 94, 269, 404, 856, 1414
HapII	CCGG	6 cut(s)	101, 117, 143, 249, 1115, 1593
HgaI	GACGC	1 cut(s)	1064
HhaI	GCGC	3 cut(s)	46, 148, 1440
Hin1I	GRCGYC	1 cut(s)	1056
Hin1II	CATG	6 cut(s)	496, 516, 821, 831, 1016, 1369
Hin6I	GCGC	3 cut(s)	44, 146, 1438
HinP1I	GCGC	3 cut(s)	44, 146, 1438
HincII	GTYRAC	4 cut(s)	139, 763, 1120, 1461
HindII	GTYRAC	4 cut(s)	139, 763, 1120, 1461
HindIII	AAGCTT	2 cut(s)	1340, 1740
HinfI	GANTC	3 cut(s)	611, 665, 1325
HpaII	CCGG	6 cut(s)	101, 117, 143, 249, 1115, 1593
HphI	GGTGA	2 cut(s)	501, 986
Hpy166II	GTNNAC	5 cut(s)	139, 468, 763, 1120, 1461
Hpy188I	TCNGA	8 cut(s)	71, 302, 319, 927, 1229, 1514, 1589, 1629
Hpy188III	TCNNGA	9 cut(s)	37, 229, 677, 845, 881, 1262, 1271, 1467, 1522
Hpy8I	GTNNAC	5 cut(s)	139, 468, 763, 1120, 1461
Hpy99I	CGWCG	3 cut(s)	161, 164, 911
HpyAV	CCTTC	7 cut(s)	187, 259, 367, 867, 961, 1144, 1307
HpyCH4III	ACNGT	7 cut(s)	136, 236, 424, 723, 1171, 1635, 1751
HpyCH4IV	ACGT	4 cut(s)	83, 591, 651, 909
HpyF10VI	GCNNNNNNNGC	6 cut(s)	1055, 1379, 1427, 1430, 1446, 1574
HpyF3I	CTNAG	3 cut(s)	57, 1374, 1780
HpySE526I	ACGT	4 cut(s)	83, 591, 651, 909
Hsp92I	GRCGYC	1 cut(s)	1056
Hsp92II	CATG	6 cut(s)	496, 516, 821, 831, 1016, 1369
HspAI	GCGC	3 cut(s)	44, 146, 1438
KroI	GCCGGC	1 cut(s)	100
KroNI	GCCGGC	1 cut(s)	102
Ksp22I	TGATCA	1 cut(s)	1216
Kzo9I	GATC	7 cut(s)	205, 328, 847, 1216, 1384, 1589, 1612
LmnI	GCTCC	3 cut(s)	1446, 1552, 1582
Lsp1109I	GCAGC	4 cut(s)	1408, 1417, 1436, 1439
LweI	GCATC	4 cut(s)	736, 792, 1276, 1505
MaeI	CTAG	3 cut(s)	677, 777, 1271
MaeII	ACGT	4 cut(s)	83, 591, 651, 909
MaeIII	GTNAC	6 cut(s)	244, 507, 1003, 1491, 1617, 1751
MalI	GATC	7 cut(s)	207, 330, 849, 1218, 1386, 1591, 1614
MbiI	CCGCTC	1 cut(s)	1547
MboI	GATC	7 cut(s)	205, 328, 847, 1216, 1384, 1589, 1612
MfeI	CAATTG	3 cut(s)	461, 585, 1655
MflI	RGATCY	3 cut(s)	328, 1589, 1612
MhlI	GDGCHC	4 cut(s)	78, 836, 1451, 1579
MlyI	GAGTC	1 cut(s)	674
MmeI	TCCRAC	2 cut(s)	297, 1567
Mph1103I	ATGCAT	2 cut(s)	751, 1367
MroNI	GCCGGC	1 cut(s)	100
MroXI	GAANNNNTTC	1 cut(s)	1743
MseI	TTAA	5 cut(s)	333, 597, 633, 984, 1029
MslI	CAYNNNNRTG	3 cut(s)	826, 969, 1272
MspA1I	CMGCKG	1 cut(s)	1430
MspCI	CTTAAG	1 cut(s)	1028
MspI	CCGG	6 cut(s)	101, 117, 143, 249, 1115, 1593
MspR9I	CCNGG	5 cut(s)	143, 250, 813, 1115, 1594
MunI	CAATTG	3 cut(s)	461, 585, 1655
Mva1269I	GAATGC	1 cut(s)	921
MvaI	CCWGG	1 cut(s)	813
MvnI	CGCG	1 cut(s)	44
MwoI	GCNNNNNNNGC	6 cut(s)	1055, 1379, 1427, 1430, 1446, 1574
NaeI	GCCGGC	1 cut(s)	102
NciI	CCSGG	4 cut(s)	143, 250, 1115, 1594
NcoI	CCATGG	1 cut(s)	512
NdeI	CATATG	2 cut(s)	622, 751
NdeII	GATC	7 cut(s)	205, 328, 847, 1216, 1384, 1589, 1612
NgoMIV	GCCGGC	1 cut(s)	100
NlaIII	CATG	6 cut(s)	496, 516, 821, 831, 1016, 1369
NlaIV	GGNNCC	4 cut(s)	76, 1112, 1591, 1614
NmeAIII	GCCGAG	1 cut(s)	1477
NmuCI	GTSAC	3 cut(s)	507, 1617, 1751
NsiI	ATGCAT	2 cut(s)	751, 1367
NspI	RCATGY	2 cut(s)	1016, 1369
OliI	CACNNNNGTG	1 cut(s)	969
PaeR7I	CTCGAG	1 cut(s)	1416
PciI	ACATGT	1 cut(s)	1012
PctI	GAATGC	1 cut(s)	921
PdiI	GCCGGC	1 cut(s)	102
PdmI	GAANNNNTTC	1 cut(s)	1743
PfeI	GAWTC	2 cut(s)	611, 1325
PflMI	CCANNNNNTGG	2 cut(s)	702, 1107
PkrI	GCNGC	5 cut(s)	1423, 1426, 1429, 1432, 1546
PleI	GAGTC	1 cut(s)	673
PpsI	GAGTC	1 cut(s)	673
Ppu21I	YACGTR	1 cut(s)	84
PscI	ACATGT	1 cut(s)	1012
PshAI	GACNNNNGTC	1 cut(s)	1115
Psp6I	CCWGG	1 cut(s)	811
PspEI	GGTNACC	1 cut(s)	244
PspFI	CCCAGC	1 cut(s)	996
PspGI	CCWGG	1 cut(s)	811
PspN4I	GGNNCC	4 cut(s)	76, 1112, 1591, 1614
PspOMI	GGGCCC	1 cut(s)	74
PspPI	GGNCC	3 cut(s)	74, 75, 1110
PspXI	VCTCGAGB	1 cut(s)	1416
PstI	CTGCAG	2 cut(s)	1426, 1435
PstNI	CAGNNNCTG	1 cut(s)	1430
PsuI	RGATCY	3 cut(s)	328, 1589, 1612
PvuII	CAGCTG	1 cut(s)	1430
RsaI	GTAC	4 cut(s)	212, 469, 501, 684
RsaNI	GTAC	4 cut(s)	211, 468, 500, 683
RseI	CAYNNNNRTG	3 cut(s)	826, 969, 1272
SalI	GTCGAC	1 cut(s)	137
SaqAI	TTAA	5 cut(s)	333, 597, 633, 984, 1029
SatI	GCNGC	5 cut(s)	1422, 1425, 1428, 1431, 1545
Sau3AI	GATC	7 cut(s)	205, 328, 847, 1216, 1384, 1589, 1612
Sau96I	GGNCC	3 cut(s)	74, 75, 1110
SchI	GAGTC	1 cut(s)	674
ScrFI	CCNGG	5 cut(s)	143, 250, 813, 1115, 1594
SduI	GDGCHC	4 cut(s)	78, 836, 1451, 1579
SfaNI	GCATC	4 cut(s)	736, 792, 1276, 1505
SfcI	CTRYAG	3 cut(s)	1170, 1422, 1431
Sfr274I	CTCGAG	1 cut(s)	1416
SinI	GGWCC	1 cut(s)	1110
SlaI	CTCGAG	1 cut(s)	1416
SmiMI	CAYNNNNRTG	3 cut(s)	826, 969, 1272
SmlI	CTYRAG	2 cut(s)	1028, 1416
SmoI	CTYRAG	2 cut(s)	1028, 1416
SsiI	CCGC	5 cut(s)	104, 165, 439, 1191, 1545
SspI	AATATT	1 cut(s)	1067
SspMI	CTAG	3 cut(s)	677, 777, 1271
StyD4I	CCNGG	5 cut(s)	141, 248, 811, 1113, 1592
StyI	CCWWGG	5 cut(s)	270, 512, 533, 1280, 1348
TaaI	ACNGT	7 cut(s)	136, 236, 424, 723, 1171, 1635, 1751
TaiI	ACGT	4 cut(s)	86, 594, 654, 912
TaqI	TCGA	9 cut(s)	38, 138, 547, 614, 906, 954, 1417, 1570, 1604
TatI	WGTACW	2 cut(s)	467, 499
TauI	GCSGC	1 cut(s)	1547
TfiI	GAWTC	2 cut(s)	611, 1325
Tru1I	TTAA	5 cut(s)	333, 597, 633, 984, 1029
Tru9I	TTAA	5 cut(s)	333, 597, 633, 984, 1029
TscAI	CASTG	4 cut(s)	429, 728, 841, 1672
TseFI	GTSAC	3 cut(s)	507, 1617, 1751
TseI	GCWGC	4 cut(s)	1421, 1424, 1427, 1430
Tsp45I	GTSAC	3 cut(s)	507, 1617, 1751
TspDTI	ATGAA	6 cut(s)	278, 495, 632, 768, 993, 1497
TspGWI	ACGGA	2 cut(s)	172, 1605
TspRI	CASTG	4 cut(s)	429, 728, 841, 1672
Van91I	CCANNNNNTGG	2 cut(s)	702, 1107
Vha464I	CTTAAG	1 cut(s)	1028
VpaK11BI	GGWCC	1 cut(s)	1110
XapI	RAATTY	1 cut(s)	895
XbaI	TCTAGA	2 cut(s)	676, 1270
XceI	RCATGY	2 cut(s)	1016, 1369
XhoI	CTCGAG	1 cut(s)	1416
XmiI	GTMKAC	1 cut(s)	138
XmnI	GAANNNNTTC	1 cut(s)	1743
XspI	CTAG	3 cut(s)	677, 777, 1271
Zsp2I	ATGCAT	2 cut(s)	751, 1367

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.