MD07G1190100.v1.1

Beta-hexosaminidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013206
Physical Location & Seq
Forward (+)
27044521 .. 27050544
6024 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1190100.v1.1.491

Sequence Viewer

Length: 1617 bp
ATGTCCTCCTTGTTCCTGAGCCTGTTTATAATCTCCCATTCCCTCTGTGTTTTGGTTAATTCAGCCGGCGAAGTCAACGACTCTCTGACGTTGTTATGGCCGCTGCCGGCCGAGTTCACCTTCGGGAACAAGACCCTCTCCGTCGACCCAGCGCTCTCGCTGGTTGTTGGCGGGAACGGCGGCGGCTCCGGCATTCTGAAGCTGGGTTTTGATCGGTACAGAGAAATTATATTCAAGAACAATCATGGGGTTTTAGCTTTGAATACACTGAGGGGAAGTAGACAGAGTTATGATATTAGTAAGCTGAGAATTGTGGTTGAATCGGCCGACGAAGATCTTCAACTTGGTGTGGATGAGAGCTATACTTTGTTTGTGGTGAAGAATGATGGCAAATCGATTGTTGGCGAGGCGACGATCGAGGCGAACACTGTGTATGGTGCTCTGCGGGCTTTGGAGACATTCAGCCAGTTGTGTACTTTTGATTATGGGAGTAAATCTGTACAAGTAAATAAGGCACCCTGGTACATAAAGGATAGTCCAAGGTTTGCATACCGAGGGCTTTTGATTGATACATCAAGGCACTATTTACCAATTGAAGTGATTAAGCAAGTGATCCAGTCAATGTCGTATGCTAAACTTAATGTTCTTCACTGGCACGTCATAGATAGAGAGTCTTTTCCTCTAGAAGTACCTTCATATCCAAAATTGTGGAATGGTGCGTACACAAAATGGGAGCGCTACACGGTTGAGGATGCAATTGAAATTGTCAACTTTGCCAAAACAAGAGGCATCAATGTTATGGCAGAAGTAGATGTCCCTGGTCATGCAGAGTCATGGGGCACTGGGTATCCTGATCTTTGGCCTTCCCCTTCCTGCAGAGAGCCACTTGATGTTTCGAAGGACTTAAGTTTAGATGTGATTTCTGGAATTCTGACAGATATGAGAAAGATTTTTCCCTTCGAGCTGTTCCACTTGGGCGGTGATGAAGTTAATACAACTTGTTGGAACACCACTCAACATGTGAAGCAATGGCTTGAAGAGCGAAACATGACTACTAAAGATGCATATCAGTATTTTGTTATTAAAGCACAAGAAATAGCAATTTCAAAGAAGTGGACCCCAGTAAACTGGGAAGAAACCTTCAATACCTTCCCATCGAAGCTCAATCCCAAGACCGTAGTGCATAACTGGTTGGGCTCAGGGGTTTGTCCGAAGGCGGTTGCCAAAGGTTTCAGATGTATTTTCAGCAATCAGGGTGTTTGGTATCTTGACCATCTAGATGTCCCTTGGGATGTTACGTACGATGCTGAACCACTTGAAGGAATTACTGATATTTCTCAGCAAAAGCTTGTTATCGGAGGAGAAGTATGCATGTGGGGGGAGACAGCTGATACATCAGATGTTCAGCAAACAATTTGGCCTCGAGCTGCAGCTGCTGCAGAACGCTTGTGGAGCAGGAGGGGGGTAACATCTCGTGGAAATAGTTATGCAACAGCATTATCTCGGCTACAATACTTTAGATGTCTCTTAAACAGACGTGGAGTTCAAGCCGCTCCAGTAACAAACTTCTATGCTCGAAGTCCGCCAACTTGGTCTGGATCATGCTACGATCAATAA

Protein Analysis

539

Amino Acids

60.51

Weight (kDa)

5.88

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycohydro_20b2 PF14845 32 - 157 1.6e-24 beta-acetyl hexosaminidase like
Glyco_hydro_20 PF00728 182 - 486 5.2e-78 Glycosyl hydrolase family 20, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013206)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65590 AT3G55260
fragaria_vesca FvH4_7g21240
malus_domestica MD01G1120700.v1.1 MD07G1190100.v1.1
prunus_persica Prupe.2G227600_v2.0.a1 Prupe.2G227600_v2.0.a1 Prupe.2G228000_v2.0.a1
pyrus_communis pycom01g14730 pycom07g17970
rosa_chinensis RchiOBHm_Chr1g0365681
rosa_laevigata RLG00000027418
rosa_roxburghii Rroxscaffold_4G00291090
rosa_samantha Rh1AG331300 Rh1BG292800 Rh1CG309300 Rh1DG324300
rosa_wichuraiana Rw1G029430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 29
AccB1I GGYRCC 1 cut(s) 514
AccBSI CCGCTC 1 cut(s) 1553
AccI GTMKAC 2 cut(s) 144, 280
AciI CCGC 9 cut(s) 101, 171, 180, 183, 445, 978, 1217, 1551, 1583
AclWI GGATC 2 cut(s) 607, 1606
AcoI YGGCCR 3 cut(s) 98, 108, 324
AcsI RAATTY 1 cut(s) 927
AcuI CTGAAG 1 cut(s) 218
AfaI GTAC 7 cut(s) 218, 475, 501, 524, 690, 722, 1301
AfeI AGCGCT 2 cut(s) 153, 737
AfiI CCNNNNNNNGG 1 cut(s) 1319
AflII CTTAAG 1 cut(s) 904
AflIII ACRYGT 1 cut(s) 1018
AjiI CACGTC 2 cut(s) 658, 1538
AjnI CCWGG 2 cut(s) 518, 817
Alw21I GWGCWC 1 cut(s) 442
Alw26I GTCTC 3 cut(s) 449, 1376, 1529
AlwI GGATC 2 cut(s) 607, 1606
AlwNI CAGNNNCTG 1 cut(s) 1436
Ama87I CYCGRG 1 cut(s) 1422
Aor51HI AGCGCT 2 cut(s) 153, 737
AoxI GGCC 5 cut(s) 98, 108, 324, 860, 1418
ApeKI GCWGC 5 cut(s) 103, 1427, 1430, 1433, 1436
ApoI RAATTY 1 cut(s) 927
Asp700I GAANNNNTTC 1 cut(s) 336
AspLEI GCGC 2 cut(s) 154, 738
AspS9I GGNCC 1 cut(s) 1116
AsuHPI GGTGA 3 cut(s) 109, 388, 992
AsuII TTCGAA 1 cut(s) 896
AvaI CYCGRG 1 cut(s) 1422
AvaII GGWCC 1 cut(s) 1116
BaeGI GKGCMC 1 cut(s) 842
BanI GGYRCC 1 cut(s) 514
BanII GRGCYC 1 cut(s) 1199
BauI CACGAG 1 cut(s) 1473
Bbv12I GWGCWC 1 cut(s) 442
BbvI GCAGC 5 cut(s) 90, 1414, 1420, 1423, 1442
BccI CCATC 3 cut(s) 380, 1162, 1281
BceAI ACGGC 1 cut(s) 193
BciT130I CCWGG 2 cut(s) 520, 819
BciVI GTATCC 1 cut(s) 858
BcoDI GTCTC 3 cut(s) 449, 1376, 1529
BfaI CTAG 2 cut(s) 683, 1277
BfmI CTRYAG 3 cut(s) 874, 1428, 1437
BfoI RGCGCY 2 cut(s) 155, 739
BfrI CTTAAG 1 cut(s) 904
BfuI GTATCC 1 cut(s) 858
BglII AGATCT 1 cut(s) 334
BisI GCNGC 9 cut(s) 101, 104, 181, 184, 1428, 1431, 1434, 1437, 1551
BlsI GCNGC 9 cut(s) 102, 105, 182, 185, 1429, 1432, 1435, 1438, 1552
Bme1390I CCNGG 2 cut(s) 520, 819
Bme18I GGWCC 1 cut(s) 1116
BmeT110I CYCGRG 1 cut(s) 1422
BmgBI CACGTC 2 cut(s) 658, 1538
BmgT120I GGNCC 1 cut(s) 1116
BmiI GGNNCC 3 cut(s) 187, 516, 1118
BmrFI CCNGG 2 cut(s) 520, 819
BmrI ACTGGG 3 cut(s) 852, 1115, 1138
BmsI GCATC 4 cut(s) 742, 798, 1051, 1294
BmuI ACTGGG 3 cut(s) 852, 1115, 1138
BpmI CTGGAG 1 cut(s) 1539
Bpu10I CCTNAGC 2 cut(s) 17, 1198
Bpu14I TTCGAA 1 cut(s) 896
Bsa29I ATCGAT 1 cut(s) 395
BsaAI YACGTR 1 cut(s) 1299
BsaBI GATNNNNATC 1 cut(s) 1065
BsaJI CCNNGG 5 cut(s) 518, 539, 553, 817, 1286
BsaXI ACNNNNNCTCC 2 cut(s) 1350, 1380
Bsc4I CCNNNNNNNGG 1 cut(s) 1319
Bse118I RCCGGY 2 cut(s) 65, 106
Bse1I ACTGG 8 cut(s) 466, 616, 656, 847, 1121, 1133, 1193, 1556
Bse3DI GCAATG 1 cut(s) 1034
Bse8I GATNNNNATC 1 cut(s) 1065
BseBI CCWGG 2 cut(s) 520, 819
BseCI ATCGAT 1 cut(s) 395
BseDI CCNNGG 5 cut(s) 518, 539, 553, 817, 1286
BseGI GGATG 3 cut(s) 358, 757, 1297
BseJI GATNNNNATC 1 cut(s) 1065
BseLI CCNNNNNNNGG 1 cut(s) 1319
BseMI GCAATG 1 cut(s) 1034
BseMII CTCAG 5 cut(s) 8, 260, 296, 1212, 1352
BseNI ACTGG 8 cut(s) 466, 616, 656, 847, 1121, 1133, 1193, 1556
BseRI GAGGAG 1 cut(s) 1374
BseSI GKGCMC 1 cut(s) 842
BseX3I CGGCCG 2 cut(s) 108, 324
BseXI GCAGC 5 cut(s) 90, 1414, 1420, 1423, 1442
BseYI CCCAGC 2 cut(s) 148, 202
Bsh1285I CGRYCG 3 cut(s) 111, 327, 417
BshFI GGCC 5 cut(s) 100, 110, 326, 862, 1420
BshNI GGYRCC 1 cut(s) 514
BshVI ATCGAT 1 cut(s) 395
BsiEI CGRYCG 3 cut(s) 111, 327, 417
BsiHKAI GWGCWC 1 cut(s) 442
BsiHKCI CYCGRG 1 cut(s) 1422
BsiSI CCGG 3 cut(s) 66, 107, 189
BsiWI CGTACG 1 cut(s) 1299
BslFI GGGAC 2 cut(s) 800, 1268
BslI CCNNNNNNNGG 1 cut(s) 1319
BsmAI GTCTC 3 cut(s) 449, 1376, 1529
BsmFI GGGAC 2 cut(s) 800, 1268
BsmI GAATGC 1 cut(s) 192
BsnI GGCC 5 cut(s) 100, 110, 326, 862, 1420
BsoBI CYCGRG 1 cut(s) 1422
Bsp119I TTCGAA 1 cut(s) 896
Bsp1286I GDGCHC 3 cut(s) 442, 842, 1199
Bsp1407I TGTACA 1 cut(s) 499
Bsp143I GATC 7 cut(s) 211, 334, 414, 612, 853, 1598, 1609
BspACI CCGC 9 cut(s) 101, 171, 180, 183, 445, 978, 1217, 1551, 1583
BspANI GGCC 5 cut(s) 100, 110, 326, 862, 1420
BspCNI CTCAG 5 cut(s) 9, 261, 297, 1211, 1351
BspDI ATCGAT 1 cut(s) 395
BspLI GGNNCC 3 cut(s) 187, 516, 1118
BspMAI CTGCAG 3 cut(s) 878, 1432, 1441
BspPI GGATC 2 cut(s) 607, 1606
BspQI GCTCTTC 1 cut(s) 1032
BspT104I TTCGAA 1 cut(s) 896
BspT107I GGYRCC 1 cut(s) 514
BspTI CTTAAG 1 cut(s) 904
BsrBI CCGCTC 1 cut(s) 1553
BsrDI GCAATG 1 cut(s) 1034
BsrFI RCCGGY 2 cut(s) 65, 106
BsrGI TGTACA 1 cut(s) 499
BsrI ACTGG 8 cut(s) 466, 616, 656, 847, 1121, 1133, 1193, 1556
BssAI RCCGGY 2 cut(s) 65, 106
BssECI CCNNGG 5 cut(s) 518, 539, 553, 817, 1286
BssMI GATC 7 cut(s) 211, 334, 414, 612, 853, 1598, 1609
BssSI CACGAG 1 cut(s) 1473
BssT1I CCWWGG 2 cut(s) 539, 1286
Bst2BI CACGAG 1 cut(s) 1473
Bst2UI CCWGG 2 cut(s) 520, 819
Bst4CI ACNGT 3 cut(s) 430, 745, 1177
Bst6I CTCTTC 1 cut(s) 1032
BstAFI CTTAAG 1 cut(s) 904
BstAPI GCANNNNNTGC 1 cut(s) 1436
BstAUI TGTACA 1 cut(s) 499
BstBAI YACGTR 1 cut(s) 1299
BstBI TTCGAA 1 cut(s) 896
BstC8I GCNNGC 3 cut(s) 67, 108, 447
BstDEI CTNAG 5 cut(s) 17, 269, 305, 1198, 1338
BstF5I GGATG 3 cut(s) 358, 757, 1297
BstH2I RGCGCY 2 cut(s) 155, 739
BstHHI GCGC 2 cut(s) 154, 738
BstKTI GATC 7 cut(s) 214, 337, 417, 615, 856, 1601, 1612
BstMAI GTCTC 3 cut(s) 449, 1376, 1529
BstMBI GATC 7 cut(s) 211, 334, 414, 612, 853, 1598, 1609
BstMCI CGRYCG 3 cut(s) 111, 327, 417
BstMWI GCNNNNNNNGC 7 cut(s) 177, 189, 446, 1039, 1433, 1436, 1452
BstNI CCWGG 2 cut(s) 520, 819
BstNSI RCATGY 2 cut(s) 1022, 1375
BstSCI CCNGG 2 cut(s) 518, 817
BstSFI CTRYAG 3 cut(s) 874, 1428, 1437
BstSLI GKGCMC 1 cut(s) 842
BstSNI TACGTA 1 cut(s) 1299
BstV1I GCAGC 5 cut(s) 90, 1414, 1420, 1423, 1442
BstX2I RGATCY 1 cut(s) 334
BstXI CCANNNNNNTGG 2 cut(s) 708, 1128
BstYI RGATCY 1 cut(s) 334
BstZI CGGCCG 2 cut(s) 108, 324
Bsu15I ATCGAT 1 cut(s) 395
BsuI GTATCC 1 cut(s) 858
BsuRI GGCC 5 cut(s) 100, 110, 326, 862, 1420
BsuTUI ATCGAT 1 cut(s) 395
BtrI CACGTC 2 cut(s) 658, 1538
BtsCI GGATG 3 cut(s) 358, 757, 1297
BtsIMutI CAGTG 4 cut(s) 266, 426, 649, 840
Cac8I GCNNGC 3 cut(s) 67, 108, 447
CaiI CAGNNNCTG 1 cut(s) 1436
CfoI GCGC 2 cut(s) 154, 738
Cfr10I RCCGGY 2 cut(s) 65, 106
Cfr13I GGNCC 1 cut(s) 1116
ClaI ATCGAT 1 cut(s) 395
Csp6I GTAC 7 cut(s) 217, 474, 500, 523, 689, 721, 1300
CviAII CATG 7 cut(s) 245, 824, 834, 1019, 1048, 1372, 1602
CviQI GTAC 7 cut(s) 217, 474, 500, 523, 689, 721, 1300
DdeI CTNAG 5 cut(s) 17, 269, 305, 1198, 1338
DpnI GATC 7 cut(s) 213, 336, 416, 614, 855, 1600, 1611
DpnII GATC 7 cut(s) 211, 334, 414, 612, 853, 1598, 1609
EaeI YGGCCR 3 cut(s) 98, 108, 324
EagI CGGCCG 2 cut(s) 108, 324
Eam1104I CTCTTC 1 cut(s) 1032
EarI CTCTTC 1 cut(s) 1032
EciI GGCGGA 1 cut(s) 1572
EclXI CGGCCG 2 cut(s) 108, 324
Eco105I TACGTA 1 cut(s) 1299
Eco130I CCWWGG 2 cut(s) 539, 1286
Eco24I GRGCYC 1 cut(s) 1199
Eco47I GGWCC 1 cut(s) 1116
Eco47III AGCGCT 2 cut(s) 153, 737
Eco52I CGGCCG 2 cut(s) 108, 324
Eco57I CTGAAG 1 cut(s) 218
Eco88I CYCGRG 1 cut(s) 1422
EcoRI GAATTC 1 cut(s) 927
EcoRII CCWGG 2 cut(s) 518, 817
EcoT14I CCWWGG 2 cut(s) 539, 1286
EcoT22I ATGCAT 2 cut(s) 1066, 1373
EcoT38I GRGCYC 1 cut(s) 1199
ErhI CCWWGG 2 cut(s) 539, 1286
FaeI CATG 7 cut(s) 248, 827, 837, 1022, 1051, 1375, 1605
FaqI GGGAC 2 cut(s) 800, 1268
FatI CATG 7 cut(s) 244, 823, 833, 1018, 1047, 1371, 1601
FauI CCCGC 2 cut(s) 164, 438
FblI GTMKAC 2 cut(s) 144, 280
Fnu4HI GCNGC 9 cut(s) 101, 104, 181, 184, 1428, 1431, 1434, 1437, 1551
FokI GGATG 3 cut(s) 365, 764, 1304
FriOI GRGCYC 1 cut(s) 1199
Fsp4HI GCNGC 9 cut(s) 101, 104, 181, 184, 1428, 1431, 1434, 1437, 1551
FspBI CTAG 2 cut(s) 683, 1277
GlaI GCGC 2 cut(s) 153, 737
GluI GCNGC 9 cut(s) 101, 104, 181, 184, 1428, 1431, 1434, 1437, 1551
GsaI CCCAGC 2 cut(s) 152, 206
GsuI CTGGAG 1 cut(s) 1539
HaeII RGCGCY 2 cut(s) 155, 739
HaeIII GGCC 5 cut(s) 100, 110, 326, 862, 1420
HapII CCGG 3 cut(s) 66, 107, 189
HhaI GCGC 2 cut(s) 154, 738
Hin1II CATG 7 cut(s) 248, 827, 837, 1022, 1051, 1375, 1605
Hin6I GCGC 2 cut(s) 152, 736
HinP1I GCGC 2 cut(s) 152, 736
HincII GTYRAC 3 cut(s) 76, 145, 769
HindII GTYRAC 3 cut(s) 76, 145, 769
HindIII AAGCTT 1 cut(s) 1346
HinfI GANTC 4 cut(s) 80, 320, 671, 830
HpaII CCGG 3 cut(s) 66, 107, 189
HphI GGTGA 3 cut(s) 109, 388, 992
Hpy166II GTNNAC 9 cut(s) 76, 117, 145, 281, 474, 723, 769, 1116, 1126
Hpy188I TCNGA 7 cut(s) 87, 198, 933, 1212, 1235, 1358, 1399
Hpy188III TCNNGA 9 cut(s) 16, 124, 235, 683, 851, 924, 1268, 1277, 1596
Hpy8I GTNNAC 9 cut(s) 76, 117, 145, 281, 474, 723, 769, 1116, 1126
Hpy99I CGWCG 3 cut(s) 146, 332, 415
HpyCH4III ACNGT 3 cut(s) 430, 745, 1177
HpyCH4IV ACGT 4 cut(s) 89, 657, 1298, 1537
HpyF10VI GCNNNNNNNGC 7 cut(s) 177, 189, 446, 1039, 1433, 1436, 1452
HpyF3I CTNAG 5 cut(s) 17, 269, 305, 1198, 1338
HpySE526I ACGT 4 cut(s) 89, 657, 1298, 1537
Hsp92II CATG 7 cut(s) 248, 827, 837, 1022, 1051, 1375, 1605
HspAI GCGC 2 cut(s) 152, 736
KroI GCCGGC 2 cut(s) 65, 106
KroNI GCCGGC 2 cut(s) 67, 108
Kzo9I GATC 7 cut(s) 211, 334, 414, 612, 853, 1598, 1609
LguI GCTCTTC 1 cut(s) 1032
LmnI GCTCC 4 cut(s) 191, 733, 1452, 1558
Lsp1109I GCAGC 5 cut(s) 90, 1414, 1420, 1423, 1442
LweI GCATC 4 cut(s) 742, 798, 1051, 1294
MaeI CTAG 2 cut(s) 683, 1277
MaeII ACGT 4 cut(s) 89, 657, 1298, 1537
MaeIII GTNAC 3 cut(s) 1294, 1465, 1558
MalI GATC 7 cut(s) 213, 336, 416, 614, 855, 1600, 1611
MbiI CCGCTC 1 cut(s) 1553
MboI GATC 7 cut(s) 211, 334, 414, 612, 853, 1598, 1609
MboII GAAGA 6 cut(s) 329, 344, 391, 638, 1049, 1145
MfeI CAATTG 2 cut(s) 591, 756
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 3 cut(s) 442, 842, 1199
MlyI GAGTC 3 cut(s) 74, 680, 839
MmeI TCCRAC 1 cut(s) 983
Mph1103I ATGCAT 2 cut(s) 1066, 1373
MroNI GCCGGC 2 cut(s) 65, 106
MroXI GAANNNNTTC 1 cut(s) 336
MseI TTAA 7 cut(s) 57, 603, 639, 905, 990, 1083, 1529
MslI CAYNNNNRTG 1 cut(s) 1278
MspA1I CMGCKG 3 cut(s) 103, 1388, 1433
MspCI CTTAAG 1 cut(s) 904
MspI CCGG 3 cut(s) 66, 107, 189
MspR9I CCNGG 2 cut(s) 520, 819
MunI CAATTG 2 cut(s) 591, 756
Mva1269I GAATGC 1 cut(s) 192
MvaI CCWGG 2 cut(s) 520, 819
MwoI GCNNNNNNNGC 7 cut(s) 177, 189, 446, 1039, 1433, 1436, 1452
NaeI GCCGGC 2 cut(s) 67, 108
NdeII GATC 7 cut(s) 211, 334, 414, 612, 853, 1598, 1609
NgoMIV GCCGGC 2 cut(s) 65, 106
NlaIII CATG 7 cut(s) 248, 827, 837, 1022, 1051, 1375, 1605
NlaIV GGNNCC 3 cut(s) 187, 516, 1118
NmeAIII GCCGAG 2 cut(s) 136, 1483
NsiI ATGCAT 2 cut(s) 1066, 1373
NspI RCATGY 2 cut(s) 1022, 1375
NspV TTCGAA 1 cut(s) 896
PaeR7I CTCGAG 1 cut(s) 1422
PciI ACATGT 1 cut(s) 1018
PciSI GCTCTTC 1 cut(s) 1032
PcsI WCGNNNNNNNCGW 1 cut(s) 419
PctI GAATGC 1 cut(s) 192
PdiI GCCGGC 2 cut(s) 67, 108
PdmI GAANNNNTTC 1 cut(s) 336
PfeI GAWTC 1 cut(s) 320
Pfl23II CGTACG 1 cut(s) 1299
PkrI GCNGC 9 cut(s) 102, 105, 182, 185, 1429, 1432, 1435, 1438, 1552
Ple19I CGATCG 1 cut(s) 417
PleI GAGTC 3 cut(s) 74, 679, 838
PpsI GAGTC 3 cut(s) 74, 679, 838
Ppu21I YACGTR 1 cut(s) 1299
PscI ACATGT 1 cut(s) 1018
PsiI TTATAA 1 cut(s) 29
Psp6I CCWGG 2 cut(s) 518, 817
PspFI CCCAGC 2 cut(s) 148, 202
PspGI CCWGG 2 cut(s) 518, 817
PspLI CGTACG 1 cut(s) 1299
PspN4I GGNNCC 3 cut(s) 187, 516, 1118
PspPI GGNCC 1 cut(s) 1116
PspXI VCTCGAGB 1 cut(s) 1422
PstI CTGCAG 3 cut(s) 878, 1432, 1441
PstNI CAGNNNCTG 1 cut(s) 1436
PsuI RGATCY 1 cut(s) 334
PvuI CGATCG 1 cut(s) 417
PvuII CAGCTG 2 cut(s) 1388, 1433
RsaI GTAC 7 cut(s) 218, 475, 501, 524, 690, 722, 1301
RsaNI GTAC 7 cut(s) 217, 474, 500, 523, 689, 721, 1300
RseI CAYNNNNRTG 1 cut(s) 1278
SalI GTCGAC 1 cut(s) 143
SapI GCTCTTC 1 cut(s) 1032
SaqAI TTAA 7 cut(s) 57, 603, 639, 905, 990, 1083, 1529
SatI GCNGC 9 cut(s) 101, 104, 181, 184, 1428, 1431, 1434, 1437, 1551
Sau3AI GATC 7 cut(s) 211, 334, 414, 612, 853, 1598, 1609
Sau96I GGNCC 1 cut(s) 1116
SchI GAGTC 3 cut(s) 74, 680, 839
ScrFI CCNGG 2 cut(s) 520, 819
SduI GDGCHC 3 cut(s) 442, 842, 1199
SfaNI GCATC 4 cut(s) 742, 798, 1051, 1294
SfcI CTRYAG 3 cut(s) 874, 1428, 1437
Sfr274I CTCGAG 1 cut(s) 1422
SfuI TTCGAA 1 cut(s) 896
SinI GGWCC 1 cut(s) 1116
SlaI CTCGAG 1 cut(s) 1422
SmiMI CAYNNNNRTG 1 cut(s) 1278
SmlI CTYRAG 2 cut(s) 904, 1422
SmoI CTYRAG 2 cut(s) 904, 1422
SnaBI TACGTA 1 cut(s) 1299
SsiI CCGC 9 cut(s) 101, 171, 180, 183, 445, 978, 1217, 1551, 1583
SspMI CTAG 2 cut(s) 683, 1277
StyD4I CCNGG 2 cut(s) 518, 817
StyI CCWWGG 2 cut(s) 539, 1286
TaaI ACNGT 3 cut(s) 430, 745, 1177
TaiI ACGT 4 cut(s) 92, 660, 1301, 1540
TaqI TCGA 8 cut(s) 144, 395, 417, 896, 960, 1157, 1423, 1576
TatI WGTACW 2 cut(s) 473, 499
TauI GCSGC 4 cut(s) 103, 183, 186, 1553
TfiI GAWTC 1 cut(s) 320
Tru1I TTAA 7 cut(s) 57, 603, 639, 905, 990, 1083, 1529
Tru9I TTAA 7 cut(s) 57, 603, 639, 905, 990, 1083, 1529
TscAI CASTG 4 cut(s) 273, 433, 656, 847
TseI GCWGC 5 cut(s) 103, 1427, 1430, 1433, 1436
TspDTI ATGAA 2 cut(s) 684, 999
TspGWI ACGGA 1 cut(s) 130
TspRI CASTG 4 cut(s) 273, 433, 656, 847
Vha464I CTTAAG 1 cut(s) 904
VpaK11BI GGWCC 1 cut(s) 1116
XapI RAATTY 1 cut(s) 927
XbaI TCTAGA 2 cut(s) 682, 1276
XceI RCATGY 2 cut(s) 1022, 1375
XhoI CTCGAG 1 cut(s) 1422
XmiI GTMKAC 2 cut(s) 144, 280
XmnI GAANNNNTTC 1 cut(s) 336
XspI CTAG 2 cut(s) 683, 1277
Zsp2I ATGCAT 2 cut(s) 1066, 1373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.