Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0006100

Physical Location & Seq

Genomic Coordinates Reverse (-)

26481618 .. 26486841

Gene Structure 5224 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1158000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2190 bp

ATGCTTCTTCCCCGTCTTCGCCCCTATATTAGTTGCCAGACCATAATCTCTCTCCTCAGTCTCCTCCGCGGCAAAGCCCAAGTCTTCTTCCCCTTCTTCAAAATGTCCCAACCCGCCACCGCCCCGGACCACCAATCCTCCGGCCCGACTCCTCCACCGTTCGACCCGACCCAACCCGCCGTTCCCATCTCCTACCCGATCAAAACTCTCGAAGACCTCGAGTCCAGGTCCTACTTCGAGTCCTTTCACTACCCTTTTAACAAATCCACGGTTCCTCTGCAATCCGCGTCGCCTTCCCTGTCGCTGCCCGACAGGCCCAGAGTGATTGTCTGCCATGACATGGCGGGCGGCTATGGCGATGATAAGTGGATCCAGGGAGGGACCAATCCGAACGCGTATGCGATATGGCATTGGTATTTGATCGATATATTCATCTACTTTTCCCATAGCCTTGTCACTCTTCCGCCTCCGTGTTGGACTAATACTGCTCACAGACATGGAGTTAAGGTGTTGGGGACTTTCATCACAGAATGGGATGAAGGGAAGCTTATTTGCAACAAATTGCTGTCGACCAAAGAGTCTGCTGAAATGTATGCCGAGTTCTTGGCAGAGCTTGTCGTTGCTTTGGGCTTCGACGGATGGCTGATCAATATGGAGGTTGAGTTGAACTCGGACCAAATCCCTAATTTGAAAGCATTTGTCAGCCATTTAACCCAGACAATGCATTCCTCTGTGCCTGGTTCTTTGGTGATATGGTATGATAGTGTTACAACTGATGGTAAACTTAACTGGCAAAATCAACTGAATGAAAAGAATAAACCTTTCTTCGATATATGTGATGGAATTTTCGTGAACTATACATGGAAGGAAACCTATCCGAGGCTTTCCGCAGCTGTTGCTGGTGACAGAAAGTATGATGTCTACATGGGAATTGATGTTTTTGGAAGGGGCACTTTCGGTGGTGGACAATGGAATACAAGTGTTGCACTAGATGTGCTGAAGAAGGATGGTGTATCAACGGCCATATTTGCTCCCGGATGGATCTATGAGACTAATCAACCACCAAATTTTCAGATTGCTCAGAATCATTGGTGGTCCCTTGTGGAAAAATCATGGGGAGTGGTACAAAATTATCCGAAAGTGCTACCATTCTATACAAATTTTGATCAGGGTTATGGATATCATGTTTCTGTCGACGGAGGGCAAGTATCCGATGCTTCTTGGTGTAACATTTCTTCCCAAGGGTTTCAGCCGTTCCTTGAGTTTAGTGACAATGCAACCCCAGATGGCATCCAGGTCCATGTTGATTTCAAGGAAGCATCTTATAGTGGAGGAGGAAACATCACATTTAAAGGAAACCTTGAAGACTCTGATAATTTCTCGACTAGGCTCTTTCAAGGAGATGTTCTTTTGGGGGATTTACCTCTCCACTTTACGTATTCTGTGAAATCGGAGAATAATTCTCGTCTAGGCTTGCATCTTGTATTCTCTTCTTCTACCGTGAACGAAAGAAAGTCGGTGCTTCTTGTAAACTGGAACCTGAACCAGTTCTCAAGCAAATTTGATAAAGTGATAATGACACGTCAACCTGAAACGCCAGGAATTTCCCCTGGATGGGTCATGCAGAAGAGTAGCATCCGAATGAATGGATACAGACTAACAGAAATACATGCTCTGTGCTACAGGTCGAAGCCTGAATTTGACGAAAGGAGACTGAAGTCCATATCAGAGGAAGGTACTCGGAATTCAACAGAGTACTATGCAGTGCTTGGTCATATTTCAATTAAGACTTGTGGGCAGAAATCAGATTTTCCGCCTTCTGATTCGTGGCTTGTCAAAGGTGAATATATCAAATGGGCAACAGGATCTGAGGGTTCCAAGAGCGTTAGTCTTAAAATCACTTGGAAGTTGAAAGACGGGAACGATTATCCATACACAAATTATAATATTTATGTTGAAAAGCTAGCAAAGGGATCACTTGGCCATCAAGGAGCAGCGCTAGAAAGGGTGCATGAGTATCTTGGAGCGGCACGAGTGGAAGCTTTTTACGTCGATGACCTCGCAGTCACTTCTGGCACTTCTAACATCAAGTTTATCGTTCAAGTGTGTGGTGCCGACGGGAGTAACCAGAAACTAGAAGACTCGCCGGTGTTTCTACTTGAGGCTGCAGCTTTGGCTAACGAGTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000270 GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0004563 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005975 GO:0006022 GO:0006026 GO:0006027 GO:0006464 GO:0006491 GO:0006517 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009100 GO:0009253 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0015929 GO:0016052 GO:0016231 GO:0016787 GO:0016798 GO:0019538 GO:0030203 GO:0033925 GO:0035821 GO:0036211 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044033 GO:0044035 GO:0044040 GO:0044041 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044278 GO:0044419 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051672 GO:0051704 GO:0051707 GO:0071704 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Glyco_hydro_85

Protein Analysis

730

Amino Acids

81.71

Weight (kDa)

5.74

Isoelectric Point (pI)

35.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_85	PF03644	118 - 395	1.3e-102	Glycosyl hydrolase family 85
Ig_ENGASE1_C	PF25529	603 - 721	2.4e-29	ENGASE1-like, C-terminal immunoglobulin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006100)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11040 AT3G11040 AT3G11040 AT3G61010 AT3G61010 AT3G61010 AT5G05460
fragaria_vesca	FvH4_7g25120 FvH4_7g25120 FvH4_7g25120 FvH4_7g25120
malus_domestica	MD01G1158000.v1.1 MD07G1226700.v1.1
prunus_persica	Prupe.2G256300_v2.0.a1
pyrus_communis	pycom01g17460 pycom01g17470 pycom07g20530
rosa_chinensis	RchiOBHm_Chr1g0370971
rosa_laevigata	RLG00000026994
rosa_multiflora	Rmu_sc0001018.1_g000014
rosa_roxburghii	Rroxscaffold_4G00286070
rosa_rugosa	Rorug01G0360400
rosa_samantha	Rh1AG370800 Rh1BG334400 Rh1CG347700 Rh1DG365600
rosa_wichuraiana	Rw1G032630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1944
AasI	GACNNNNNNGTC	2 cut(s)	577, 2063
AccB1I	GGYRCC	1 cut(s)	2111
AccB7I	CCANNNNNTGG	1 cut(s)	340
AccBSI	CCGCTC	1 cut(s)	2027
AccI	GTMKAC	3 cut(s)	569, 921, 1194
AccII	CGCG	3 cut(s)	69, 287, 395
AclWI	GGATC	5 cut(s)	364, 377, 1049, 1873, 1981
AcoI	YGGCCR	2 cut(s)	1020, 1981
AcsI	RAATTY	7 cut(s)	843, 1066, 1159, 1559, 1602, 1697, 1744
AcuI	CTGAAG	2 cut(s)	1019, 1736
AfaI	GTAC	3 cut(s)	1125, 1738, 1757
AfeI	AGCGCT	1 cut(s)	1998
AfiI	CCNNNNNNNGG	4 cut(s)	340, 879, 1614, 1615
AflIII	ACRYGT	2 cut(s)	393, 1580
AhdI	GACNNNNNGTC	1 cut(s)	220
AjiI	CACGTC	1 cut(s)	1583
AjnI	CCWGG	6 cut(s)	224, 372, 736, 1293, 1597, 1609
AjuI	GAANNNNNNNTTGG	2 cut(s)	165, 197
AluBI	AGCT	6 cut(s)	547, 613, 893, 1963, 2042, 2171
AluI	AGCT	6 cut(s)	547, 613, 893, 1963, 2042, 2171
Alw26I	GTCTC	3 cut(s)	65, 1043, 1705
AlwI	GGATC	5 cut(s)	364, 377, 1049, 1873, 1981
AlwNI	CAGNNNCTG	1 cut(s)	1868
Ama87I	CYCGRG	1 cut(s)	218
Aor51HI	AGCGCT	1 cut(s)	1998
AoxI	GGCC	4 cut(s)	142, 314, 1020, 1981
ApeKI	GCWGC	5 cut(s)	304, 890, 1994, 2165, 2168
ApoI	RAATTY	7 cut(s)	843, 1066, 1159, 1559, 1602, 1697, 1744
ArsI	GACNNNNNNTTYG	2 cut(s)	66, 98
Asp700I	GAANNNNTTC	1 cut(s)	1547
AspLEI	GCGC	1 cut(s)	1999
AspS9I	GGNCC	8 cut(s)	127, 143, 228, 315, 381, 673, 1095, 1297
AsuC2I	CCSGG	2 cut(s)	125, 1035
AsuHPI	GGTGA	3 cut(s)	760, 914, 1853
AsuNHI	GCTAGC	1 cut(s)	1963
AvaI	CYCGRG	1 cut(s)	218
AvaII	GGWCC	6 cut(s)	127, 228, 381, 673, 1095, 1297
BaeGI	GKGCMC	1 cut(s)	953
BalI	TGGCCA	1 cut(s)	1983
BamHI	GGATCC	1 cut(s)	369
BanI	GGYRCC	1 cut(s)	2111
BarI	GAAGNNNNNNTAC	2 cut(s)	1219, 1251
BauI	CACGAG	1 cut(s)	2031
BbsI	GAAGAC	5 cut(s)	8, 76, 219, 1371, 2145
BbvI	GCAGC	5 cut(s)	291, 902, 2006, 2152, 2180
BccI	CCATC	9 cut(s)	194, 633, 770, 833, 1001, 1032, 1280, 1608, 1992
BceAI	ACGGC	3 cut(s)	164, 1035, 1237
BciT130I	CCWGG	6 cut(s)	226, 374, 738, 1295, 1599, 1611
BciVI	GTATCC	2 cut(s)	1219, 1643
BclI	TGATCA	2 cut(s)	645, 1165
BcnI	CCSGG	2 cut(s)	125, 1035
BcoDI	GTCTC	3 cut(s)	65, 1043, 1705
BfaI	CTAG	6 cut(s)	989, 1386, 1469, 1964, 2000, 2135
BfmI	CTRYAG	2 cut(s)	1681, 2166
BfoI	RGCGCY	1 cut(s)	2000
BfuI	GTATCC	2 cut(s)	1219, 1643
BglI	GCCNNNNNGGC	1 cut(s)	313
BisI	GCNGC	8 cut(s)	70, 305, 349, 891, 1995, 2028, 2166, 2169
BlsI	GCNGC	8 cut(s)	71, 306, 350, 892, 1996, 2029, 2167, 2170
BmcAI	AGTACT	1 cut(s)	1757
Bme1390I	CCNGG	8 cut(s)	125, 226, 374, 738, 1035, 1295, 1599, 1611
Bme18I	GGWCC	6 cut(s)	127, 228, 381, 673, 1095, 1297
BmeRI	GACNNNNNGTC	1 cut(s)	220
BmeT110I	CYCGRG	1 cut(s)	218
BmgBI	CACGTC	1 cut(s)	1583
BmgT120I	GGNCC	8 cut(s)	127, 143, 228, 315, 381, 673, 1095, 1297
BmiI	GGNNCC	7 cut(s)	273, 371, 382, 1097, 1538, 1876, 2113
BmrFI	CCNGG	8 cut(s)	125, 226, 374, 738, 1035, 1295, 1599, 1611
BmsI	GCATC	5 cut(s)	1204, 1299, 1328, 1486, 1644
BmtI	GCTAGC	1 cut(s)	1967
BoxI	GACNNNNGTC	1 cut(s)	1717
BpiI	GAAGAC	5 cut(s)	8, 76, 219, 1371, 2145
BplI	GAGNNNNNCTC	2 cut(s)	653, 685
BpuEI	CTTGAG	3 cut(s)	1280, 1537, 2180
BpuMI	CCSGG	2 cut(s)	125, 1035
Bsa29I	ATCGAT	1 cut(s)	423
BsaAI	YACGTR	1 cut(s)	1437
BsaJI	CCNNGG	7 cut(s)	67, 123, 267, 373, 878, 1240, 1609
BsaXI	ACNNNNNCTCC	8 cut(s)	122, 152, 492, 522, 647, 677, 1191, 1221
Bsc4I	CCNNNNNNNGG	4 cut(s)	340, 879, 1614, 1615
Bse118I	RCCGGY	1 cut(s)	2146
Bse1I	ACTGG	3 cut(s)	794, 1538, 1546
BseBI	CCWGG	6 cut(s)	226, 374, 738, 1295, 1599, 1611
BseCI	ATCGAT	1 cut(s)	423
BseDI	CCNNGG	7 cut(s)	67, 123, 267, 373, 878, 1240, 1609
BseGI	GGATG	7 cut(s)	541, 644, 1012, 1043, 1290, 1619, 1635
BseLI	CCNNNNNNNGG	4 cut(s)	340, 879, 1614, 1615
BseMII	CTCAG	3 cut(s)	70, 1094, 1860
BseNI	ACTGG	3 cut(s)	794, 1538, 1546
BseRI	GAGGAG	4 cut(s)	44, 53, 141, 1347
BseSI	GKGCMC	1 cut(s)	953
BseXI	GCAGC	5 cut(s)	291, 902, 2006, 2152, 2180
Bsh1236I	CGCG	3 cut(s)	69, 287, 395
BshFI	GGCC	4 cut(s)	144, 316, 1022, 1983
BshNI	GGYRCC	1 cut(s)	2111
BshVI	ATCGAT	1 cut(s)	423
BsiHKCI	CYCGRG	1 cut(s)	218
BsiSI	CCGG	4 cut(s)	125, 141, 1035, 2147
BslFI	GGGAC	4 cut(s)	91, 394, 529, 1081
BslI	CCNNNNNNNGG	4 cut(s)	340, 879, 1614, 1615
BsmAI	GTCTC	3 cut(s)	65, 1043, 1705
BsmFI	GGGAC	4 cut(s)	91, 394, 529, 1081
BsmI	GAATGC	1 cut(s)	724
BsnI	GGCC	4 cut(s)	144, 316, 1022, 1983
BsoBI	CYCGRG	1 cut(s)	218
Bsp1286I	GDGCHC	1 cut(s)	953
Bsp143I	GATC	8 cut(s)	198, 369, 420, 645, 1041, 1165, 1865, 1973
BspANI	GGCC	4 cut(s)	144, 316, 1022, 1983
BspCNI	CTCAG	3 cut(s)	69, 1093, 1861
BspDI	ATCGAT	1 cut(s)	423
BspFNI	CGCG	3 cut(s)	69, 287, 395
BspLI	GGNNCC	7 cut(s)	273, 371, 382, 1097, 1538, 1876, 2113
BspMAI	CTGCAG	1 cut(s)	2170
BspOI	GCTAGC	1 cut(s)	1967
BspPI	GGATC	5 cut(s)	364, 377, 1049, 1873, 1981
BspT107I	GGYRCC	1 cut(s)	2111
BsrBI	CCGCTC	1 cut(s)	2027
BsrFI	RCCGGY	1 cut(s)	2146
BsrI	ACTGG	3 cut(s)	794, 1538, 1546
BssAI	RCCGGY	1 cut(s)	2146
BssECI	CCNNGG	7 cut(s)	67, 123, 267, 373, 878, 1240, 1609
BssMI	GATC	8 cut(s)	198, 369, 420, 645, 1041, 1165, 1865, 1973
BssSI	CACGAG	1 cut(s)	2031
BssT1I	CCWWGG	1 cut(s)	1240
Bst2BI	CACGAG	1 cut(s)	2031
Bst2UI	CCWGG	6 cut(s)	226, 374, 738, 1295, 1599, 1611
Bst4CI	ACNGT	3 cut(s)	159, 271, 1501
Bst6I	CTCTTC	3 cut(s)	465, 1495, 1622
BstAPI	GCANNNNNTGC	1 cut(s)	896
BstBAI	YACGTR	1 cut(s)	1437
BstC8I	GCNNGC	3 cut(s)	346, 1475, 1965
BstDEI	CTNAG	3 cut(s)	56, 1080, 1869
BstDSI	CCRYGG	2 cut(s)	67, 267
BstENI	CCTNNNNNAGG	1 cut(s)	877
BstF5I	GGATG	7 cut(s)	541, 644, 1012, 1043, 1290, 1619, 1635
BstFNI	CGCG	3 cut(s)	69, 287, 395
BstH2I	RGCGCY	1 cut(s)	2000
BstHHI	GCGC	1 cut(s)	1999
BstKTI	GATC	8 cut(s)	201, 372, 423, 648, 1044, 1168, 1868, 1976
BstMAI	GTCTC	3 cut(s)	65, 1043, 1705
BstMBI	GATC	8 cut(s)	198, 369, 420, 645, 1041, 1165, 1865, 1973
BstMWI	GCNNNNNNNGC	5 cut(s)	313, 354, 896, 1028, 2174
BstNI	CCWGG	6 cut(s)	226, 374, 738, 1295, 1599, 1611
BstNSI	RCATGY	1 cut(s)	1673
BstPAI	GACNNNNGTC	1 cut(s)	1717
BstSCI	CCNGG	8 cut(s)	123, 224, 372, 736, 1033, 1293, 1597, 1609
BstSFI	CTRYAG	2 cut(s)	1681, 2166
BstSLI	GKGCMC	1 cut(s)	953
BstSNI	TACGTA	1 cut(s)	1437
BstUI	CGCG	3 cut(s)	69, 287, 395
BstV1I	GCAGC	5 cut(s)	291, 902, 2006, 2152, 2180
BstV2I	GAAGAC	5 cut(s)	8, 76, 219, 1371, 2145
BstX2I	RGATCY	3 cut(s)	369, 1041, 1865
BstYI	RGATCY	3 cut(s)	369, 1041, 1865
Bsu15I	ATCGAT	1 cut(s)	423
BsuI	GTATCC	2 cut(s)	1219, 1643
BsuRI	GGCC	4 cut(s)	144, 316, 1022, 1983
BsuTUI	ATCGAT	1 cut(s)	423
BtgI	CCRYGG	2 cut(s)	67, 267
BtgZI	GCGATG	1 cut(s)	372
BtrI	CACGTC	1 cut(s)	1583
BtsCI	GGATG	7 cut(s)	541, 644, 1012, 1043, 1290, 1619, 1635
BtsI	GCAGTG	1 cut(s)	1770
BtsIMutI	CAGTG	1 cut(s)	1770
Cac8I	GCNNGC	3 cut(s)	346, 1475, 1965
CaiI	CAGNNNCTG	1 cut(s)	1868
CfoI	GCGC	1 cut(s)	1999
Cfr10I	RCCGGY	1 cut(s)	2146
Cfr13I	GGNCC	8 cut(s)	127, 143, 228, 315, 381, 673, 1095, 1297
Cfr42I	CCGCGG	1 cut(s)	70
ClaI	ATCGAT	1 cut(s)	423
CseI	GACGC	1 cut(s)	276
Csp6I	GTAC	3 cut(s)	1124, 1737, 1756
CviQI	GTAC	3 cut(s)	1124, 1737, 1756
DdeI	CTNAG	3 cut(s)	56, 1080, 1869
DpnI	GATC	8 cut(s)	200, 371, 422, 647, 1043, 1167, 1867, 1975
DpnII	GATC	8 cut(s)	198, 369, 420, 645, 1041, 1165, 1865, 1973
DraI	TTTAAA	1 cut(s)	1351
DrdI	GACNNNNNNGTC	2 cut(s)	577, 2063
DriI	GACNNNNNGTC	1 cut(s)	220
DseDI	GACNNNNNNGTC	2 cut(s)	577, 2063
EaeI	YGGCCR	2 cut(s)	1020, 1981
Eam1104I	CTCTTC	3 cut(s)	465, 1495, 1622
Eam1105I	GACNNNNNGTC	1 cut(s)	220
EarI	CTCTTC	3 cut(s)	465, 1495, 1622
EciI	GGCGGA	2 cut(s)	453, 1803
Eco105I	TACGTA	1 cut(s)	1437
Eco130I	CCWWGG	1 cut(s)	1240
Eco32I	GATATC	1 cut(s)	1181
Eco47I	GGWCC	6 cut(s)	127, 228, 381, 673, 1095, 1297
Eco47III	AGCGCT	1 cut(s)	1998
Eco57I	CTGAAG	2 cut(s)	1019, 1736
Eco88I	CYCGRG	1 cut(s)	218
EcoNI	CCTNNNNNAGG	1 cut(s)	877
EcoO109I	RGGNCCY	1 cut(s)	228
EcoRI	GAATTC	1 cut(s)	1744
EcoRII	CCWGG	6 cut(s)	224, 372, 736, 1293, 1597, 1609
EcoRV	GATATC	1 cut(s)	1181
EcoT14I	CCWWGG	1 cut(s)	1240
EcoT22I	ATGCAT	1 cut(s)	726
ErhI	CCWWGG	1 cut(s)	1240
FalI	AAGNNNNNCTT	6 cut(s)	531, 563, 937, 969, 1344, 1376
FaqI	GGGAC	4 cut(s)	91, 394, 529, 1081
FauI	CCCGC	3 cut(s)	121, 184, 337
FbaI	TGATCA	2 cut(s)	645, 1165
FblI	GTMKAC	3 cut(s)	569, 921, 1194
Fnu4HI	GCNGC	8 cut(s)	70, 305, 349, 891, 1995, 2028, 2166, 2169
FokI	GGATG	7 cut(s)	548, 651, 1019, 1050, 1277, 1622, 1626
Fsp4HI	GCNGC	8 cut(s)	70, 305, 349, 891, 1995, 2028, 2166, 2169
FspBI	CTAG	6 cut(s)	989, 1386, 1469, 1964, 2000, 2135
GlaI	GCGC	1 cut(s)	1998
GluI	GCNGC	8 cut(s)	70, 305, 349, 891, 1995, 2028, 2166, 2169
HaeII	RGCGCY	1 cut(s)	2000
HaeIII	GGCC	4 cut(s)	144, 316, 1022, 1983
HapII	CCGG	4 cut(s)	125, 141, 1035, 2147
HgaI	GACGC	1 cut(s)	276
HhaI	GCGC	1 cut(s)	1999
Hin6I	GCGC	1 cut(s)	1997
HinP1I	GCGC	1 cut(s)	1997
HincII	GTYRAC	3 cut(s)	570, 1195, 1586
HindII	GTYRAC	3 cut(s)	570, 1195, 1586
HindIII	AAGCTT	2 cut(s)	545, 2040
HinfI	GANTC	8 cut(s)	148, 221, 239, 578, 1084, 1367, 1823, 2141
HpaII	CCGG	4 cut(s)	125, 141, 1035, 2147
HphI	GGTGA	3 cut(s)	760, 914, 1853
Hpy166II	GTNNAC	9 cut(s)	570, 782, 853, 922, 965, 1195, 1504, 1531, 1586
Hpy188III	TCNNGA	3 cut(s)	209, 850, 1381
Hpy8I	GTNNAC	9 cut(s)	570, 782, 853, 922, 965, 1195, 1504, 1531, 1586
Hpy99I	CGWCG	5 cut(s)	292, 638, 1199, 2054, 2120
HpyAV	CCTTC	8 cut(s)	103, 303, 533, 859, 939, 997, 1727, 1827
HpyCH4III	ACNGT	3 cut(s)	159, 271, 1501
HpyCH4IV	ACGT	3 cut(s)	1436, 1582, 2049
HpyF10VI	GCNNNNNNNGC	5 cut(s)	313, 354, 896, 1028, 2174
HpyF3I	CTNAG	3 cut(s)	56, 1080, 1869
HpySE526I	ACGT	3 cut(s)	1436, 1582, 2049
HspAI	GCGC	1 cut(s)	1997
Ksp22I	TGATCA	2 cut(s)	645, 1165
KspI	CCGCGG	1 cut(s)	70
Kzo9I	GATC	8 cut(s)	198, 369, 420, 645, 1041, 1165, 1865, 1973
LmnI	GCTCC	3 cut(s)	1036, 1991, 2024
Lsp1109I	GCAGC	5 cut(s)	291, 902, 2006, 2152, 2180
LweI	GCATC	5 cut(s)	1204, 1299, 1328, 1486, 1644
MaeI	CTAG	6 cut(s)	989, 1386, 1469, 1964, 2000, 2135
MaeII	ACGT	3 cut(s)	1436, 1582, 2049
MaeIII	GTNAC	7 cut(s)	454, 766, 902, 1226, 1268, 2065, 2123
MalI	GATC	8 cut(s)	200, 371, 422, 647, 1043, 1167, 1867, 1975
MbiI	CCGCTC	1 cut(s)	2027
MboI	GATC	8 cut(s)	198, 369, 420, 645, 1041, 1165, 1865, 1973
MflI	RGATCY	3 cut(s)	369, 1041, 1865
MhlI	GDGCHC	1 cut(s)	953
MlsI	TGGCCA	1 cut(s)	1983
MluI	ACGCGT	1 cut(s)	393
MluNI	TGGCCA	1 cut(s)	1983
MlyI	GAGTC	6 cut(s)	142, 230, 248, 587, 1361, 2135
MmeI	TCCRAC	1 cut(s)	455
Mox20I	TGGCCA	1 cut(s)	1983
Mph1103I	ATGCAT	1 cut(s)	726
MroXI	GAANNNNTTC	1 cut(s)	1547
MscI	TGGCCA	1 cut(s)	1983
MseI	TTAA	7 cut(s)	258, 504, 710, 786, 1350, 1785, 1893
MslI	CAYNNNNRTG	2 cut(s)	495, 1640
Msp20I	TGGCCA	1 cut(s)	1983
MspA1I	CMGCKG	2 cut(s)	69, 893
MspI	CCGG	4 cut(s)	125, 141, 1035, 2147
MspR9I	CCNGG	8 cut(s)	125, 226, 374, 738, 1035, 1295, 1599, 1611
Mva1269I	GAATGC	1 cut(s)	724
MvaI	CCWGG	6 cut(s)	226, 374, 738, 1295, 1599, 1611
MvnI	CGCG	3 cut(s)	69, 287, 395
MwoI	GCNNNNNNNGC	5 cut(s)	313, 354, 896, 1028, 2174
NciI	CCSGG	2 cut(s)	125, 1035
NdeII	GATC	8 cut(s)	198, 369, 420, 645, 1041, 1165, 1865, 1973
NheI	GCTAGC	1 cut(s)	1963
NlaIV	GGNNCC	7 cut(s)	273, 371, 382, 1097, 1538, 1876, 2113
NmeAIII	GCCGAG	1 cut(s)	622
NmuCI	GTSAC	4 cut(s)	454, 902, 1268, 2065
NsiI	ATGCAT	1 cut(s)	726
NspI	RCATGY	1 cut(s)	1673
PaeR7I	CTCGAG	1 cut(s)	218
PcsI	WCGNNNNNNNCGW	1 cut(s)	216
PctI	GAATGC	1 cut(s)	724
PdmI	GAANNNNTTC	1 cut(s)	1547
PfeI	GAWTC	2 cut(s)	1084, 1823
PflMI	CCANNNNNTGG	1 cut(s)	340
PfoI	TCCNGGA	1 cut(s)	1033
PkrI	GCNGC	8 cut(s)	71, 306, 350, 892, 1996, 2029, 2167, 2170
PleI	GAGTC	6 cut(s)	142, 229, 247, 586, 1361, 2135
PpsI	GAGTC	6 cut(s)	142, 229, 247, 586, 1361, 2135
Ppu21I	YACGTR	1 cut(s)	1437
PpuMI	RGGWCCY	1 cut(s)	228
PshAI	GACNNNNGTC	1 cut(s)	1717
PsiI	TTATAA	1 cut(s)	1944
Psp5II	RGGWCCY	1 cut(s)	228
Psp6I	CCWGG	6 cut(s)	224, 372, 736, 1293, 1597, 1609
PspGI	CCWGG	6 cut(s)	224, 372, 736, 1293, 1597, 1609
PspN4I	GGNNCC	7 cut(s)	273, 371, 382, 1097, 1538, 1876, 2113
PspPI	GGNCC	8 cut(s)	127, 143, 228, 315, 381, 673, 1095, 1297
PspPPI	RGGWCCY	1 cut(s)	228
PspXI	VCTCGAGB	1 cut(s)	218
PsrI	GAACNNNNNNTAC	2 cut(s)	584, 616
PstI	CTGCAG	1 cut(s)	2170
PstNI	CAGNNNCTG	1 cut(s)	1868
PsuI	RGATCY	3 cut(s)	369, 1041, 1865
PvuII	CAGCTG	1 cut(s)	893
RsaI	GTAC	3 cut(s)	1125, 1738, 1757
RsaNI	GTAC	3 cut(s)	1124, 1737, 1756
RseI	CAYNNNNRTG	2 cut(s)	495, 1640
SacII	CCGCGG	1 cut(s)	70
SalI	GTCGAC	2 cut(s)	568, 1193
SaqAI	TTAA	7 cut(s)	258, 504, 710, 786, 1350, 1785, 1893
SatI	GCNGC	8 cut(s)	70, 305, 349, 891, 1995, 2028, 2166, 2169
Sau3AI	GATC	8 cut(s)	198, 369, 420, 645, 1041, 1165, 1865, 1973
Sau96I	GGNCC	8 cut(s)	127, 143, 228, 315, 381, 673, 1095, 1297
ScaI	AGTACT	1 cut(s)	1757
SchI	GAGTC	6 cut(s)	142, 230, 248, 587, 1361, 2135
ScrFI	CCNGG	8 cut(s)	125, 226, 374, 738, 1035, 1295, 1599, 1611
SduI	GDGCHC	1 cut(s)	953
SfaNI	GCATC	5 cut(s)	1204, 1299, 1328, 1486, 1644
SfcI	CTRYAG	2 cut(s)	1681, 2166
Sfr274I	CTCGAG	1 cut(s)	218
Sfr303I	CCGCGG	1 cut(s)	70
SgrAI	CRCCGGYG	1 cut(s)	2146
SgrBI	CCGCGG	1 cut(s)	70
SinI	GGWCC	6 cut(s)	127, 228, 381, 673, 1095, 1297
SlaI	CTCGAG	1 cut(s)	218
SmiMI	CAYNNNNRTG	2 cut(s)	495, 1640
SmlI	CTYRAG	4 cut(s)	218, 1259, 1552, 2159
SmoI	CTYRAG	4 cut(s)	218, 1259, 1552, 2159
SnaBI	TACGTA	1 cut(s)	1437
SspI	AATATT	1 cut(s)	1948
SspMI	CTAG	6 cut(s)	989, 1386, 1469, 1964, 2000, 2135
StyD4I	CCNGG	8 cut(s)	123, 224, 372, 736, 1033, 1293, 1597, 1609
StyI	CCWWGG	1 cut(s)	1240
TaaI	ACNGT	3 cut(s)	159, 271, 1501
TaiI	ACGT	3 cut(s)	1439, 1585, 2052
TatI	WGTACW	1 cut(s)	1755
TauI	GCSGC	3 cut(s)	72, 351, 2030
TfiI	GAWTC	2 cut(s)	1084, 1823
Tru1I	TTAA	7 cut(s)	258, 504, 710, 786, 1350, 1785, 1893
Tru9I	TTAA	7 cut(s)	258, 504, 710, 786, 1350, 1785, 1893
TscAI	CASTG	1 cut(s)	1770
TseFI	GTSAC	4 cut(s)	454, 902, 1268, 2065
TseI	GCWGC	5 cut(s)	304, 890, 1994, 2165, 2168
Tsp45I	GTSAC	4 cut(s)	454, 902, 1268, 2065
TspDTI	ATGAA	5 cut(s)	421, 511, 552, 822, 1658
TspGWI	ACGGA	3 cut(s)	459, 651, 1212
TspRI	CASTG	1 cut(s)	1770
Van91I	CCANNNNNTGG	1 cut(s)	340
VpaK11BI	GGWCC	6 cut(s)	127, 228, 381, 673, 1095, 1297
XagI	CCTNNNNNAGG	1 cut(s)	877
XapI	RAATTY	7 cut(s)	843, 1066, 1159, 1559, 1602, 1697, 1744
XceI	RCATGY	1 cut(s)	1673
XhoI	CTCGAG	1 cut(s)	218
XmiI	GTMKAC	3 cut(s)	569, 921, 1194
XmnI	GAANNNNTTC	1 cut(s)	1547
XspI	CTAG	6 cut(s)	989, 1386, 1469, 1964, 2000, 2135
ZrmI	AGTACT	1 cut(s)	1757
Zsp2I	ATGCAT	1 cut(s)	726

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.