Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0006100

Physical Location & Seq

Genomic Coordinates Reverse (-)

30231123 .. 30236011

Gene Structure 4889 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1226700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2184 bp

ATGCTTCTTCCCCGTCTTCGCGCCTATATTAGCCGCCAAACCCTAATCTCTTTCCTCAATCTCCTCCGCCTGATCCGCCGAAAAGCCCAAATAGTCTTCCCCTTCTTCGAAATGTCCGCCCCGGACCACCAATCCTCCGCCCCGACCCCTCCGCCGTTCGACCCGACCCAACCCGCCGTACCCATCTCATATCCGATCAAAACCCTCGAAGAGCTCGAGTCCAGGTCCTACTTCGAGTCCTTTCACTACCCTTTTAACAAATCCACGGTTGCTCTGCAATCCGGGTCGCCTTCCCTGTCGCTGCCCGACAGGCCCAGAGTGATTGTTTGCCACGACATGGCCGGAGGCTATGGCGATGATAAGTGGATCCAGGGAGGGACCAATCCGAACGCGTATGCGATATGGCATTGGTATTTGATCGATATTTTCATCTACTTTTCGCATAGTCTTGTCACTCTTCCGCCTCCGTGTTGGACCAATACTGCTCACAAACATGGAGTTAAGGTGTTGGGGACCTTCATCACTGAATGGGATGAAGGGAAGCTTATTTGCAACAAATTGCTGTCGACAAAAGAGTCTGCTGAAATGTATGCTGAGTTCTTGGCAGAGCTTGCCGTTGCTTTGAGCTTCGATGGATGGCTGATCAATATGGAGGTTGAATTGAAGCCGGACCAAATCCCTAATTTGAAAGCATTTGTCTGCCATTTAACACAGAAAATGCATTCCTCAGCCCCTGGTTCTTTAGTCATATGGTATGACAGTGTTACAACTGATGGTAAACTTAACTGGCAAAATCAACTGAATGAAAAGAATAAACCCTTTTTTGATATATGTGATGGAATTTTTATGAACTATACATGGAAGCAAAACTATCCGAGGCTTTCAGCAGATGTTGCTGGTGACAGAAAGTATGATGTCTACATGGGAATTGATGTATTTGGAAGGGGCAGTTTCGGTGGAGGACAATGGAATACAAGTGTTGCACTAGATGTGCTGAAAAAGGACGATGTATCAACGGCCATTTTTGCTCCTGGATGGATCTATGAGACTAATCAACCACCAAATTTTCAGATTGCTCAGAATCATTGGTGGTCCCTCGTGGAAAAATCATGGGGGGTGGTACAAAATTATCCTAAAGTGCTACCATTCTATACAAATTTTGATCAGGGTCATGGATATCATGTTTCTGTTGACGGAGGGCAAGTGTCGGATGCTTCTTGGTGTAACATTTCTTCCCAAGGGTTGCAGCCGTTCCTTGTGTTTAGTGGCAATGCAACCCCAGATGGTATCCAGGTTCATGCTGATTTCAATGAAGCATCTTATAGCGGAGGAGGAAACATCACATTTAAAGGAAACCTTGAAGACGGTGATGATTTCTCGACAAGGCTCTTTCAAGGAGATGTTCTTCTGGGGGATTTGCCTCTCCAGTTAACGTATTCTGTGAAATCAGATATTAATTCTCGACTAGGCTTGCATCTTAATTTCTCTTCTACCCTGAACGAAAGAAAGTCGGTACTTCTTGTAAACTGGAACCTGAACCAATTCTCAAGCAAATTTGATGAAGTGATAATGACACGTCAACTTGGAAACCCAGGAACTCCCCCTGGATGGGTCATACAGGAGAGTAGCATCGGAATGAATGGATACAGACTAACAGAAATACATGCTCTGTGCTACAGGTCGAAGCCTGAATTTGACGAAAGGAGACCAAAGTCCATGTCAGAGGAGGGTACTCAGAATTCAACAGAGTACTATGCAGTGCTTGGTCATATTTCAATTAAGACTAGTGGGCAGAATTCAGATTTTCCGCCTTCTGATTCATGGCTTGTCGAAGGTGAATATATCAAATGGACAACAGGATCTGAGGGTTCCAAGAGCGTTAGTCTTAAAATCACTTGGAAATTGAAAGACGGGAACGATTATCCATCCACAAATTATAATATCTATGTTGAAAAACTAGCTCAAGGAGCACTTGGCCATCCAGGAGCAGCGCCAGAAAGGGTGCATGAGTATCTTGGATCGGCACGAGTGGGAGCATTTTACGTCGATGACCTAGCAGTTCCTTCTGGCACATCTAACATCAAGTTTATCGTTCAAGTTTGCGGTACCGACGGGAGTAACCAGAAACTAGATGACTCGCCGGTGTTTCTACTTGAAGCTGCAGCTTTGGCCAACGAGTTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000270 GO:0000323 GO:0003674 GO:0003824 GO:0004553 GO:0004563 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005975 GO:0006022 GO:0006026 GO:0006027 GO:0006464 GO:0006491 GO:0006517 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009100 GO:0009253 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0015929 GO:0016052 GO:0016231 GO:0016787 GO:0016798 GO:0019538 GO:0030203 GO:0033925 GO:0035821 GO:0036211 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044033 GO:0044035 GO:0044040 GO:0044041 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044278 GO:0044419 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051672 GO:0051704 GO:0051707 GO:0071704 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00511 map00511

Pfam Domains

Protein Families

Glyco_hydro_85

Protein Analysis

728

Amino Acids

81.27

Weight (kDa)

5.38

Isoelectric Point (pI)

38.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_85	PF03644	117 - 394	1.4e-102	Glycosyl hydrolase family 85
Ig_ENGASE1_C	PF25529	601 - 719	2.1e-29	ENGASE1-like, C-terminal immunoglobulin-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006100)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11040 AT3G11040 AT3G11040 AT3G61010 AT3G61010 AT3G61010 AT5G05460
fragaria_vesca	FvH4_7g25120 FvH4_7g25120 FvH4_7g25120 FvH4_7g25120
malus_domestica	MD01G1158000.v1.1 MD07G1226700.v1.1
prunus_persica	Prupe.2G256300_v2.0.a1
pyrus_communis	pycom01g17460 pycom01g17470 pycom07g20530
rosa_chinensis	RchiOBHm_Chr1g0370971
rosa_laevigata	RLG00000026994
rosa_multiflora	Rmu_sc0001018.1_g000014
rosa_roxburghii	Rroxscaffold_4G00286070
rosa_rugosa	Rorug01G0360400
rosa_samantha	Rh1AG370800 Rh1BG334400 Rh1CG347700 Rh1DG365600
rosa_wichuraiana	Rw1G032630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1938
AasI	GACNNNNNNGTC	1 cut(s)	574
Acc65I	GGTACC	1 cut(s)	2105
AccB1I	GGYRCC	1 cut(s)	2105
AccB7I	CCANNNNNTGG	1 cut(s)	337
AccI	GTMKAC	2 cut(s)	566, 918
AccII	CGCG	2 cut(s)	21, 392
AclWI	GGATC	6 cut(s)	67, 361, 374, 1046, 1867, 2026
AcoI	YGGCCR	4 cut(s)	339, 1017, 1975, 2169
AcsI	RAATTY	7 cut(s)	840, 1063, 1156, 1553, 1691, 1738, 1795
AfaI	GTAC	6 cut(s)	180, 1122, 1515, 1732, 1751, 2107
AfiI	CCNNNNNNNGG	3 cut(s)	337, 1608, 1609
AflIII	ACRYGT	2 cut(s)	390, 1574
AhlI	ACTAGT	1 cut(s)	1784
AjiI	CACGTC	1 cut(s)	1577
AjnI	CCWGG	8 cut(s)	221, 369, 733, 1030, 1290, 1591, 1603, 1981
AluBI	AGCT	7 cut(s)	214, 544, 610, 627, 1961, 2159, 2165
AluI	AGCT	7 cut(s)	214, 544, 610, 627, 1961, 2159, 2165
Alw21I	GWGCWC	2 cut(s)	216, 1972
Alw26I	GTCTC	2 cut(s)	1040, 1699
AlwI	GGATC	6 cut(s)	67, 361, 374, 1046, 1867, 2026
AlwNI	CAGNNNCTG	2 cut(s)	734, 1862
Ama87I	CYCGRG	1 cut(s)	215
AoxI	GGCC	5 cut(s)	311, 339, 1017, 1975, 2169
ApeKI	GCWGC	5 cut(s)	301, 1246, 1988, 2159, 2162
ApoI	RAATTY	7 cut(s)	840, 1063, 1156, 1553, 1691, 1738, 1795
ArsI	GACNNNNNNTTYG	2 cut(s)	1703, 1735
AseI	ATTAAT	1 cut(s)	1455
Asp700I	GAANNNNTTC	1 cut(s)	1541
Asp718I	GGTACC	1 cut(s)	2105
AspLEI	GCGC	2 cut(s)	23, 1993
AspS9I	GGNCC	8 cut(s)	124, 225, 312, 378, 474, 513, 670, 1092
AsuC2I	CCSGG	2 cut(s)	122, 283
AsuHPI	GGTGA	3 cut(s)	911, 1379, 1847
AsuII	TTCGAA	1 cut(s)	108
AvaI	CYCGRG	1 cut(s)	215
AvaII	GGWCC	7 cut(s)	124, 225, 378, 474, 513, 670, 1092
BalI	TGGCCA	2 cut(s)	1977, 2171
BamHI	GGATCC	1 cut(s)	366
BanI	GGYRCC	1 cut(s)	2105
BanII	GRGCYC	1 cut(s)	216
BarI	GAAGNNNNNNTAC	2 cut(s)	1216, 1248
BauI	CACGAG	2 cut(s)	1097, 2025
BbsI	GAAGAC	3 cut(s)	8, 88, 1368
Bbv12I	GWGCWC	2 cut(s)	216, 1972
BbvCI	CCTCAGC	1 cut(s)	727
BbvI	GCAGC	5 cut(s)	288, 1258, 2000, 2146, 2174
BceAI	ACGGC	5 cut(s)	139, 161, 599, 1032, 1234
BciT130I	CCWGG	8 cut(s)	223, 371, 735, 1032, 1292, 1593, 1605, 1983
BciVI	GTATCC	2 cut(s)	1298, 1637
BclI	TGATCA	2 cut(s)	642, 1162
BcnI	CCSGG	2 cut(s)	122, 283
BcoDI	GTCTC	2 cut(s)	1040, 1699
BcuI	ACTAGT	1 cut(s)	1784
BfaI	CTAG	6 cut(s)	986, 1466, 1785, 1958, 2054, 2129
BfmI	CTRYAG	2 cut(s)	1675, 2160
BfoI	RGCGCY	1 cut(s)	1994
BfuI	GTATCC	2 cut(s)	1298, 1637
BglI	GCCNNNNNGGC	1 cut(s)	310
BisI	GCNGC	6 cut(s)	34, 302, 1247, 1989, 2160, 2163
BlsI	GCNGC	6 cut(s)	35, 303, 1248, 1990, 2161, 2164
BmcAI	AGTACT	1 cut(s)	1751
Bme18I	GGWCC	7 cut(s)	124, 225, 378, 474, 513, 670, 1092
BmeT110I	CYCGRG	1 cut(s)	215
BmgBI	CACGTC	1 cut(s)	1577
BmgT120I	GGNCC	8 cut(s)	124, 225, 312, 378, 474, 513, 670, 1092
BmiI	GGNNCC	7 cut(s)	368, 379, 514, 1094, 1532, 1870, 2107
BmsI	GCATC	4 cut(s)	1201, 1325, 1483, 1638
BoxI	GACNNNNGTC	1 cut(s)	1711
BpiI	GAAGAC	3 cut(s)	8, 88, 1368
BpmI	CTGGAG	1 cut(s)	1409
Bpu10I	CCTNAGC	1 cut(s)	727
Bpu14I	TTCGAA	1 cut(s)	108
BpuEI	CTTGAG	2 cut(s)	1531, 1947
BpuMI	CCSGG	2 cut(s)	122, 283
Bsa29I	ATCGAT	1 cut(s)	420
BsaI	GGTCTC	1 cut(s)	1699
BsaJI	CCNNGG	8 cut(s)	120, 264, 370, 733, 875, 1237, 1591, 1603
BsaXI	ACNNNNNCTCC	6 cut(s)	119, 149, 489, 519, 1188, 1218
Bsc4I	CCNNNNNNNGG	3 cut(s)	337, 1608, 1609
Bse118I	RCCGGY	1 cut(s)	2140
Bse1I	ACTGG	3 cut(s)	791, 1426, 1532
Bse3DI	GCAATG	1 cut(s)	1276
BseBI	CCWGG	8 cut(s)	223, 371, 735, 1032, 1292, 1593, 1605, 1983
BseCI	ATCGAT	1 cut(s)	420
BseDI	CCNNGG	8 cut(s)	120, 264, 370, 733, 875, 1237, 1591, 1603
BseGI	GGATG	7 cut(s)	538, 641, 1040, 1216, 1613, 1925, 1978
BseLI	CCNNNNNNNGG	3 cut(s)	337, 1608, 1609
BseMI	GCAATG	1 cut(s)	1276
BseMII	CTCAG	5 cut(s)	585, 741, 1091, 1748, 1854
BseNI	ACTGG	3 cut(s)	791, 1426, 1532
BseRI	GAGGAG	3 cut(s)	53, 1344, 1739
BseXI	GCAGC	5 cut(s)	288, 1258, 2000, 2146, 2174
Bsh1236I	CGCG	2 cut(s)	21, 392
BshFI	GGCC	5 cut(s)	313, 341, 1019, 1977, 2171
BshNI	GGYRCC	1 cut(s)	2105
BshVI	ATCGAT	1 cut(s)	420
BsiHKAI	GWGCWC	2 cut(s)	216, 1972
BsiHKCI	CYCGRG	1 cut(s)	215
BsiSI	CCGG	5 cut(s)	122, 282, 342, 668, 2141
BslFI	GGGAC	3 cut(s)	391, 526, 1078
BslI	CCNNNNNNNGG	3 cut(s)	337, 1608, 1609
BsmAI	GTCTC	2 cut(s)	1040, 1699
BsmFI	GGGAC	3 cut(s)	391, 526, 1078
BsmI	GAATGC	1 cut(s)	721
BsnI	GGCC	5 cut(s)	313, 341, 1019, 1977, 2171
Bso31I	GGTCTC	1 cut(s)	1699
BsoBI	CYCGRG	1 cut(s)	215
Bsp119I	TTCGAA	1 cut(s)	108
Bsp1286I	GDGCHC	2 cut(s)	216, 1972
Bsp143I	GATC	9 cut(s)	72, 195, 366, 417, 642, 1038, 1162, 1859, 2018
BspANI	GGCC	5 cut(s)	313, 341, 1019, 1977, 2171
BspCNI	CTCAG	5 cut(s)	586, 740, 1090, 1747, 1855
BspDI	ATCGAT	1 cut(s)	420
BspFNI	CGCG	2 cut(s)	21, 392
BspLI	GGNNCC	7 cut(s)	368, 379, 514, 1094, 1532, 1870, 2107
BspMAI	CTGCAG	1 cut(s)	2164
BspPI	GGATC	6 cut(s)	67, 361, 374, 1046, 1867, 2026
BspQI	GCTCTTC	1 cut(s)	204
BspT104I	TTCGAA	1 cut(s)	108
BspT107I	GGYRCC	1 cut(s)	2105
BspTNI	GGTCTC	1 cut(s)	1699
BsrDI	GCAATG	1 cut(s)	1276
BsrFI	RCCGGY	1 cut(s)	2140
BsrI	ACTGG	3 cut(s)	791, 1426, 1532
BssAI	RCCGGY	1 cut(s)	2140
BssECI	CCNNGG	8 cut(s)	120, 264, 370, 733, 875, 1237, 1591, 1603
BssMI	GATC	9 cut(s)	72, 195, 366, 417, 642, 1038, 1162, 1859, 2018
BssSI	CACGAG	2 cut(s)	1097, 2025
BssT1I	CCWWGG	1 cut(s)	1237
Bst2BI	CACGAG	2 cut(s)	1097, 2025
Bst2UI	CCWGG	8 cut(s)	223, 371, 735, 1032, 1292, 1593, 1605, 1983
Bst4CI	ACNGT	3 cut(s)	268, 761, 1367
Bst6I	CTCTTC	3 cut(s)	204, 462, 1492
BstAPI	GCANNNNNTGC	2 cut(s)	611, 893
BstBI	TTCGAA	1 cut(s)	108
BstC8I	GCNNGC	2 cut(s)	612, 1472
BstDEI	CTNAG	5 cut(s)	594, 727, 1077, 1734, 1863
BstDSI	CCRYGG	1 cut(s)	264
BstF5I	GGATG	7 cut(s)	538, 641, 1040, 1216, 1613, 1925, 1978
BstFNI	CGCG	2 cut(s)	21, 392
BstH2I	RGCGCY	1 cut(s)	1994
BstHHI	GCGC	2 cut(s)	23, 1993
BstKTI	GATC	9 cut(s)	75, 198, 369, 420, 645, 1041, 1165, 1862, 2021
BstMAI	GTCTC	2 cut(s)	1040, 1699
BstMBI	GATC	9 cut(s)	72, 195, 366, 417, 642, 1038, 1162, 1859, 2018
BstMWI	GCNNNNNNNGC	7 cut(s)	75, 310, 611, 893, 1025, 1967, 2168
BstNI	CCWGG	8 cut(s)	223, 371, 735, 1032, 1292, 1593, 1605, 1983
BstNSI	RCATGY	1 cut(s)	1667
BstPAI	GACNNNNGTC	1 cut(s)	1711
BstSFI	CTRYAG	2 cut(s)	1675, 2160
BstUI	CGCG	2 cut(s)	21, 392
BstV1I	GCAGC	5 cut(s)	288, 1258, 2000, 2146, 2174
BstV2I	GAAGAC	3 cut(s)	8, 88, 1368
BstX2I	RGATCY	3 cut(s)	366, 1038, 1859
BstYI	RGATCY	3 cut(s)	366, 1038, 1859
Bsu15I	ATCGAT	1 cut(s)	420
BsuI	GTATCC	2 cut(s)	1298, 1637
BsuRI	GGCC	5 cut(s)	313, 341, 1019, 1977, 2171
BsuTUI	ATCGAT	1 cut(s)	420
BtgI	CCRYGG	1 cut(s)	264
BtgZI	GCGATG	1 cut(s)	369
BtrI	CACGTC	1 cut(s)	1577
BtsCI	GGATG	7 cut(s)	538, 641, 1040, 1216, 1613, 1925, 1978
BtsI	GCAGTG	1 cut(s)	1764
BtsIMutI	CAGTG	3 cut(s)	522, 766, 1764
Cac8I	GCNNGC	2 cut(s)	612, 1472
CaiI	CAGNNNCTG	2 cut(s)	734, 1862
CfoI	GCGC	2 cut(s)	23, 1993
Cfr10I	RCCGGY	1 cut(s)	2140
Cfr13I	GGNCC	8 cut(s)	124, 225, 312, 378, 474, 513, 670, 1092
ClaI	ATCGAT	1 cut(s)	420
Csp6I	GTAC	6 cut(s)	179, 1121, 1514, 1731, 1750, 2106
CviQI	GTAC	6 cut(s)	179, 1121, 1514, 1731, 1750, 2106
DdeI	CTNAG	5 cut(s)	594, 727, 1077, 1734, 1863
DpnI	GATC	9 cut(s)	74, 197, 368, 419, 644, 1040, 1164, 1861, 2020
DpnII	GATC	9 cut(s)	72, 195, 366, 417, 642, 1038, 1162, 1859, 2018
DraI	TTTAAA	1 cut(s)	1348
DrdI	GACNNNNNNGTC	1 cut(s)	574
DseDI	GACNNNNNNGTC	1 cut(s)	574
EaeI	YGGCCR	4 cut(s)	339, 1017, 1975, 2169
Eam1104I	CTCTTC	3 cut(s)	204, 462, 1492
EarI	CTCTTC	3 cut(s)	204, 462, 1492
EciI	GGCGGA	7 cut(s)	56, 65, 106, 127, 141, 450, 1797
Ecl136II	GAGCTC	1 cut(s)	214
Eco130I	CCWWGG	1 cut(s)	1237
Eco24I	GRGCYC	1 cut(s)	216
Eco31I	GGTCTC	1 cut(s)	1699
Eco32I	GATATC	1 cut(s)	1178
Eco47I	GGWCC	7 cut(s)	124, 225, 378, 474, 513, 670, 1092
Eco53kI	GAGCTC	1 cut(s)	214
Eco88I	CYCGRG	1 cut(s)	215
EcoICRI	GAGCTC	1 cut(s)	214
EcoO109I	RGGNCCY	2 cut(s)	225, 513
EcoRI	GAATTC	2 cut(s)	1738, 1795
EcoRII	CCWGG	8 cut(s)	221, 369, 733, 1030, 1290, 1591, 1603, 1981
EcoRV	GATATC	1 cut(s)	1178
EcoT14I	CCWWGG	1 cut(s)	1237
EcoT22I	ATGCAT	1 cut(s)	723
EcoT38I	GRGCYC	1 cut(s)	216
ErhI	CCWWGG	1 cut(s)	1237
FalI	AAGNNNNNCTT	6 cut(s)	528, 560, 1341, 1373, 1956, 1988
FaqI	GGGAC	3 cut(s)	391, 526, 1078
FauI	CCCGC	1 cut(s)	181
FauNDI	CATATG	1 cut(s)	749
FbaI	TGATCA	2 cut(s)	642, 1162
FblI	GTMKAC	2 cut(s)	566, 918
Fnu4HI	GCNGC	6 cut(s)	34, 302, 1247, 1989, 2160, 2163
FokI	GGATG	7 cut(s)	545, 648, 1047, 1223, 1620, 1912, 1965
FriOI	GRGCYC	1 cut(s)	216
Fsp4HI	GCNGC	6 cut(s)	34, 302, 1247, 1989, 2160, 2163
FspBI	CTAG	6 cut(s)	986, 1466, 1785, 1958, 2054, 2129
GlaI	GCGC	2 cut(s)	22, 1992
GluI	GCNGC	6 cut(s)	34, 302, 1247, 1989, 2160, 2163
GsuI	CTGGAG	1 cut(s)	1409
HaeII	RGCGCY	1 cut(s)	1994
HaeIII	GGCC	5 cut(s)	313, 341, 1019, 1977, 2171
HapII	CCGG	5 cut(s)	122, 282, 342, 668, 2141
HhaI	GCGC	2 cut(s)	23, 1993
Hin6I	GCGC	2 cut(s)	21, 1991
HinP1I	GCGC	2 cut(s)	21, 1991
HincII	GTYRAC	4 cut(s)	567, 1192, 1431, 1580
HindII	GTYRAC	4 cut(s)	567, 1192, 1431, 1580
HindIII	AAGCTT	1 cut(s)	542
HinfI	GANTC	6 cut(s)	218, 236, 575, 1081, 1817, 2135
HpaI	GTTAAC	1 cut(s)	1431
HpaII	CCGG	5 cut(s)	122, 282, 342, 668, 2141
HphI	GGTGA	3 cut(s)	911, 1379, 1847
Hpy166II	GTNNAC	7 cut(s)	567, 779, 919, 1192, 1431, 1525, 1580
Hpy188III	TCNNGA	2 cut(s)	1378, 1461
Hpy8I	GTNNAC	7 cut(s)	567, 779, 919, 1192, 1431, 1525, 1580
Hpy99I	CGWCG	2 cut(s)	2048, 2114
HpyAV	CCTTC	8 cut(s)	112, 300, 526, 530, 936, 1821, 1826, 2073
HpyCH4III	ACNGT	3 cut(s)	268, 761, 1367
HpyCH4IV	ACGT	3 cut(s)	1433, 1576, 2043
HpyF10VI	GCNNNNNNNGC	7 cut(s)	75, 310, 611, 893, 1025, 1967, 2168
HpyF3I	CTNAG	5 cut(s)	594, 727, 1077, 1734, 1863
HpySE526I	ACGT	3 cut(s)	1433, 1576, 2043
HspAI	GCGC	2 cut(s)	21, 1991
KpnI	GGTACC	1 cut(s)	2109
Ksp22I	TGATCA	2 cut(s)	642, 1162
KspAI	GTTAAC	1 cut(s)	1431
Kzo9I	GATC	9 cut(s)	72, 195, 366, 417, 642, 1038, 1162, 1859, 2018
LguI	GCTCTTC	1 cut(s)	204
LmnI	GCTCC	4 cut(s)	1033, 1967, 1985, 2033
Lsp1109I	GCAGC	5 cut(s)	288, 1258, 2000, 2146, 2174
LweI	GCATC	4 cut(s)	1201, 1325, 1483, 1638
MaeI	CTAG	6 cut(s)	986, 1466, 1785, 1958, 2054, 2129
MaeII	ACGT	3 cut(s)	1433, 1576, 2043
MaeIII	GTNAC	5 cut(s)	451, 763, 899, 1223, 2117
MalI	GATC	9 cut(s)	74, 197, 368, 419, 644, 1040, 1164, 1861, 2020
MboI	GATC	9 cut(s)	72, 195, 366, 417, 642, 1038, 1162, 1859, 2018
MboII	GAAGA	9 cut(s)	8, 88, 97, 221, 449, 1224, 1373, 1397, 1479
MflI	RGATCY	3 cut(s)	366, 1038, 1859
MhlI	GDGCHC	2 cut(s)	216, 1972
MlsI	TGGCCA	2 cut(s)	1977, 2171
MluI	ACGCGT	1 cut(s)	390
MluNI	TGGCCA	2 cut(s)	1977, 2171
MlyI	GAGTC	4 cut(s)	227, 245, 584, 2129
MmeI	TCCRAC	2 cut(s)	452, 1188
Mox20I	TGGCCA	2 cut(s)	1977, 2171
Mph1103I	ATGCAT	1 cut(s)	723
MroXI	GAANNNNTTC	1 cut(s)	1541
MscI	TGGCCA	2 cut(s)	1977, 2171
MslI	CAYNNNNRTG	2 cut(s)	492, 1634
Msp20I	TGGCCA	2 cut(s)	1977, 2171
MspI	CCGG	5 cut(s)	122, 282, 342, 668, 2141
Mva1269I	GAATGC	1 cut(s)	721
MvaI	CCWGG	8 cut(s)	223, 371, 735, 1032, 1292, 1593, 1605, 1983
MvnI	CGCG	2 cut(s)	21, 392
MwoI	GCNNNNNNNGC	7 cut(s)	75, 310, 611, 893, 1025, 1967, 2168
NciI	CCSGG	2 cut(s)	122, 283
NdeI	CATATG	1 cut(s)	749
NdeII	GATC	9 cut(s)	72, 195, 366, 417, 642, 1038, 1162, 1859, 2018
NlaIV	GGNNCC	7 cut(s)	368, 379, 514, 1094, 1532, 1870, 2107
NmuCI	GTSAC	2 cut(s)	451, 899
NsiI	ATGCAT	1 cut(s)	723
NspI	RCATGY	1 cut(s)	1667
NspV	TTCGAA	1 cut(s)	108
PaeR7I	CTCGAG	1 cut(s)	215
PciSI	GCTCTTC	1 cut(s)	204
PcsI	WCGNNNNNNNCGW	1 cut(s)	213
PctI	GAATGC	1 cut(s)	721
PdmI	GAANNNNTTC	1 cut(s)	1541
PfeI	GAWTC	2 cut(s)	1081, 1817
PflMI	CCANNNNNTGG	1 cut(s)	337
PfoI	TCCNGGA	2 cut(s)	1030, 1981
PkrI	GCNGC	6 cut(s)	35, 303, 1248, 1990, 2161, 2164
PleI	GAGTC	4 cut(s)	226, 244, 583, 2129
PpsI	GAGTC	4 cut(s)	226, 244, 583, 2129
PpuMI	RGGWCCY	2 cut(s)	225, 513
PshAI	GACNNNNGTC	1 cut(s)	1711
PshBI	ATTAAT	1 cut(s)	1455
PsiI	TTATAA	1 cut(s)	1938
Psp124BI	GAGCTC	1 cut(s)	216
Psp5II	RGGWCCY	2 cut(s)	225, 513
Psp6I	CCWGG	8 cut(s)	221, 369, 733, 1030, 1290, 1591, 1603, 1981
PspGI	CCWGG	8 cut(s)	221, 369, 733, 1030, 1290, 1591, 1603, 1981
PspN4I	GGNNCC	7 cut(s)	368, 379, 514, 1094, 1532, 1870, 2107
PspPI	GGNCC	8 cut(s)	124, 225, 312, 378, 474, 513, 670, 1092
PspPPI	RGGWCCY	2 cut(s)	225, 513
PspXI	VCTCGAGB	1 cut(s)	215
PsrI	GAACNNNNNNTAC	2 cut(s)	581, 613
PstI	CTGCAG	1 cut(s)	2164
PstNI	CAGNNNCTG	2 cut(s)	734, 1862
PsuI	RGATCY	3 cut(s)	366, 1038, 1859
RsaI	GTAC	6 cut(s)	180, 1122, 1515, 1732, 1751, 2107
RsaNI	GTAC	6 cut(s)	179, 1121, 1514, 1731, 1750, 2106
RseI	CAYNNNNRTG	2 cut(s)	492, 1634
SacI	GAGCTC	1 cut(s)	216
SalI	GTCGAC	1 cut(s)	565
SapI	GCTCTTC	1 cut(s)	204
SatI	GCNGC	6 cut(s)	34, 302, 1247, 1989, 2160, 2163
Sau3AI	GATC	9 cut(s)	72, 195, 366, 417, 642, 1038, 1162, 1859, 2018
Sau96I	GGNCC	8 cut(s)	124, 225, 312, 378, 474, 513, 670, 1092
ScaI	AGTACT	1 cut(s)	1751
SchI	GAGTC	4 cut(s)	227, 245, 584, 2129
SduI	GDGCHC	2 cut(s)	216, 1972
SfaNI	GCATC	4 cut(s)	1201, 1325, 1483, 1638
SfcI	CTRYAG	2 cut(s)	1675, 2160
Sfr274I	CTCGAG	1 cut(s)	215
SfuI	TTCGAA	1 cut(s)	108
SgrAI	CRCCGGYG	1 cut(s)	2140
SinI	GGWCC	7 cut(s)	124, 225, 378, 474, 513, 670, 1092
SlaI	CTCGAG	1 cut(s)	215
SmiMI	CAYNNNNRTG	2 cut(s)	492, 1634
SmlI	CTYRAG	3 cut(s)	215, 1546, 1962
SmoI	CTYRAG	3 cut(s)	215, 1546, 1962
SpeI	ACTAGT	1 cut(s)	1784
SspMI	CTAG	6 cut(s)	986, 1466, 1785, 1958, 2054, 2129
SstI	GAGCTC	1 cut(s)	216
StyI	CCWWGG	1 cut(s)	1237
TaaI	ACNGT	3 cut(s)	268, 761, 1367
TaiI	ACGT	3 cut(s)	1436, 1579, 2046
TatI	WGTACW	1 cut(s)	1749
TauI	GCSGC	1 cut(s)	36
TfiI	GAWTC	2 cut(s)	1081, 1817
TscAI	CASTG	3 cut(s)	529, 766, 1764
TseFI	GTSAC	2 cut(s)	451, 899
TseI	GCWGC	5 cut(s)	301, 1246, 1988, 2159, 2162
Tsp45I	GTSAC	2 cut(s)	451, 899
TspGWI	ACGGA	2 cut(s)	456, 1209
TspRI	CASTG	3 cut(s)	529, 766, 1764
Van91I	CCANNNNNTGG	1 cut(s)	337
VpaK11BI	GGWCC	7 cut(s)	124, 225, 378, 474, 513, 670, 1092
VspI	ATTAAT	1 cut(s)	1455
XapI	RAATTY	7 cut(s)	840, 1063, 1156, 1553, 1691, 1738, 1795
XceI	RCATGY	1 cut(s)	1667
XhoI	CTCGAG	1 cut(s)	215
XmiI	GTMKAC	2 cut(s)	566, 918
XmnI	GAANNNNTTC	1 cut(s)	1541
XspI	CTAG	6 cut(s)	986, 1466, 1785, 1958, 2054, 2129
ZrmI	AGTACT	1 cut(s)	1751
Zsp2I	ATGCAT	1 cut(s)	723

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.