MD01G1168200.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016396
Physical Location & Seq
Reverse (-)
27263360 .. 27264631
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1168200.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGGAGCTTCCGGAAGAACTCATCGTCGACATCCTCAAACGGCTTCCCCCCAAATCCCTCGTCCGATTCAGGTGCGTCTGCAAACCATGGTGCTCTCTGATTAAAAGCCCGAGTTTCGTCCGAGCCCTTCTGAAGAAAAACCGAGCCATGTTCCAATCCGAGCTGGGAACCAGCACCCGCGTGATTTTGTCGCGTTACTGCGACACCCTTTTGTCGATGGAGTCCGAAAACAACAATGTTGTCGGGACTGTCGAGCTTGACTTCGCTCTGGTTCGGAACTTGCCGTATTACGTAAAGGGACACTCCGATGGGCTGCTCTGTGTGGTTATCAATGATGGGATCGAGGGTGTGGCTGTAATTTACAACCCGACGATCCAGGAGTACCGGAAGCTCCCTTCTCCTCAGAACTTCAGAAGTACGAGGGAGGTGCTGGGTTTGGGTTACGATGCCTCAATCGATGATTACAAGGTTGTTCGAGTCCCGTCGAACTATTGCCGGCTGAAAGTTACGGGTTACAAGCCGCAGGTCGAGGTATTGGAACTGAAAACCAATTTCTGGAGGAAGATTCCAGACGAGGATACGCCGCCGTTTTTCATTGAGCATATTTTCCAATCAACGGAAGTGAATGGCGGATTGTATTGGATGGCAGAGGACCACGATTCGGGGAGGTGTCTGATCCTGCGATTCGATTTGGCCGACGAGAAGTTTAAGGTGGTGCCGCCACCGCCAGATGAGAGTGGTCGGAGTATAGCGTGGATTGGATCTTTGAAAGACCACCTTTGTGTCGTGCACACTCGGAGGTTGAGTGATGTTCATGTTTGGGGAACAAAAGATGACAAGAACTGGAGTAAGATCATCACCACTGCGAAGTTTCCGAGGATTCCGACCAAAGACCCGTTCCTCGATTCGTTTCGGTACATGCCGCTGTCTTTTACCAAGAAGGGAGCTGTGCTGATGAGCGTTGGAGGGGAGAGGTTTGTGACATTAGATACAAAAGACAATACATTCGAACACGTCGACATCAAGGGTGCGAATCACTGGTTGCAGGAGACGGTGTATTGCGAGACTCTTGTTTCGCCCGGAGGTGGTGTGAATCCGACAGAGTCAGCGGCGGGGTTGGGGTCGGACGTTGGTGCGTCGGAGGAAGAGGACCAATCAGATGGGGAAAGCGCGAGTAATAGCCTCAGGCAATTGCTGCTTGGTTTGAAGAGGGAAGTTCATAACTTGTTGGCTTGTACCGTTAGCCACGGGAATATCAGAGGTGCGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

424

Amino Acids

47.53

Weight (kDa)

6.11

Isoelectric Point (pI)

41.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 2 - 39 3.7e-12 F-box domain
F-box-like PF12937 2 - 41 6.4e-10 F-box-like
FBA_3 PF08268 96 - 346 1.7e-21 F-box associated beta propeller domain
FBA_1 PF07734 100 - 358 1.1e-24 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016396)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g26000
malus_domestica MD01G1168200.v1.1
prunus_persica Prupe.2G264700_v2.0.a1
pyrus_communis pycom01g18120
rosa_chinensis RchiOBHm_Chr1g0372381
rosa_laevigata RLG00000026902
rosa_multiflora Rmu_sc0001755.1_g000005 Rmu_sc0032499.1_g000001
rosa_roxburghii Rroxscaffold_4G00284990
rosa_rugosa Rorug01G0371400
rosa_samantha Rh1AG380100 Rh1BG343600 Rh1CG357100 Rh1DG374800
rosa_wichuraiana Rw1G033410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 514
AccB1I GGYRCC 1 cut(s) 715
AccB7I CCANNNNNTGG 1 cut(s) 555
AccI GTMKAC 2 cut(s) 27, 1017
AccII CGCG 3 cut(s) 180, 193, 1172
AccIII TCCGGA 1 cut(s) 10
AclWI GGATC 4 cut(s) 347, 367, 670, 769
AcoI YGGCCR 1 cut(s) 693
AcuI CTGAAG 2 cut(s) 152, 394
AfaI GTAC 4 cut(s) 383, 418, 917, 1237
AfiI CCNNNNNNNGG 4 cut(s) 555, 616, 661, 1085
AflIII ACRYGT 1 cut(s) 1012
AgsI TTSAA 2 cut(s) 769, 1207
AjiI CACGTC 1 cut(s) 1015
AjnI CCWGG 1 cut(s) 375
AjuI GAANNNNNNNTTGG 2 cut(s) 881, 913
AleI CACNNNNGTG 2 cut(s) 179, 780
AluBI AGCT 5 cut(s) 7, 163, 256, 391, 947
AluI AGCT 5 cut(s) 7, 163, 256, 391, 947
Alw21I GWGCWC 2 cut(s) 95, 792
Alw26I GTCTC 2 cut(s) 1043, 1058
Alw44I GTGCAC 1 cut(s) 788
AlwI GGATC 4 cut(s) 347, 367, 670, 769
Ama87I CYCGRG 1 cut(s) 109
Aor13HI TCCGGA 1 cut(s) 10
AoxI GGCC 1 cut(s) 693
ApaLI GTGCAC 1 cut(s) 788
ApeKI GCWGC 2 cut(s) 313, 1195
ArsI GACNNNNNNTTYG 6 cut(s) 45, 77, 989, 1021, 1057, 1089
AspLEI GCGC 1 cut(s) 1172
AspS9I GGNCC 2 cut(s) 652, 1150
AsuC2I CCSGG 1 cut(s) 1080
AsuHPI GGTGA 1 cut(s) 850
AsuII TTCGAA 1 cut(s) 1008
AvaI CYCGRG 1 cut(s) 109
AvaII GGWCC 2 cut(s) 652, 1150
AxyI CCTNAGG 1 cut(s) 1184
BaeGI GKGCMC 1 cut(s) 792
BanI GGYRCC 1 cut(s) 715
BanII GRGCYC 1 cut(s) 127
Bbv12I GWGCWC 2 cut(s) 95, 792
BbvI GCAGC 2 cut(s) 300, 1182
BccI CCATC 5 cut(s) 211, 302, 329, 637, 1154
BceAI ACGGC 3 cut(s) 56, 268, 571
BcgI CGANNNNNNTGC 4 cut(s) 54, 88, 409, 443
BciT130I CCWGG 1 cut(s) 377
BciVI GTATCC 1 cut(s) 571
BcnI CCSGG 1 cut(s) 1080
BcoDI GTCTC 2 cut(s) 1043, 1058
BfuAI ACCTGC 1 cut(s) 514
BfuI GTATCC 1 cut(s) 571
BisI GCNGC 7 cut(s) 314, 521, 584, 719, 923, 1110, 1196
BlsI GCNGC 7 cut(s) 315, 522, 585, 720, 924, 1111, 1197
Bme1390I CCNGG 2 cut(s) 377, 1080
Bme18I GGWCC 2 cut(s) 652, 1150
BmeT110I CYCGRG 1 cut(s) 109
BmgBI CACGTC 1 cut(s) 1015
BmgT120I GGNCC 2 cut(s) 652, 1150
BmiI GGNNCC 2 cut(s) 169, 717
BmrFI CCNGG 2 cut(s) 377, 1080
BmsI GCATC 1 cut(s) 436
BpmI CTGGAG 2 cut(s) 577, 865
Bpu14I TTCGAA 1 cut(s) 1008
BpuMI CCSGG 1 cut(s) 1080
Bsa29I ATCGAT 1 cut(s) 456
BsaAI YACGTR 1 cut(s) 292
BsaJI CCNNGG 3 cut(s) 86, 875, 1246
BsaWI WCCGGW 2 cut(s) 10, 384
BsaXI ACNNNNNCTCC 6 cut(s) 790, 820, 1040, 1070, 1074, 1104
Bsc4I CCNNNNNNNGG 4 cut(s) 555, 616, 661, 1085
Bse118I RCCGGY 1 cut(s) 495
Bse1I ACTGG 2 cut(s) 848, 1043
Bse21I CCTNAGG 1 cut(s) 1184
BseAI TCCGGA 1 cut(s) 10
BseBI CCWGG 1 cut(s) 377
BseCI ATCGAT 1 cut(s) 456
BseDI CCNNGG 3 cut(s) 86, 875, 1246
BseGI GGATG 2 cut(s) 30, 648
BseLI CCNNNNNNNGG 4 cut(s) 555, 616, 661, 1085
BseMII CTCAG 2 cut(s) 416, 1198
BseNI ACTGG 2 cut(s) 848, 1043
BseRI GAGGAG 1 cut(s) 390
BseSI GKGCMC 1 cut(s) 792
BseXI GCAGC 2 cut(s) 300, 1182
BseYI CCCAGC 2 cut(s) 163, 430
Bsh1236I CGCG 3 cut(s) 180, 193, 1172
BshFI GGCC 1 cut(s) 695
BshNI GGYRCC 1 cut(s) 715
BshVI ATCGAT 1 cut(s) 456
BsiHKAI GWGCWC 2 cut(s) 95, 792
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 4 cut(s) 11, 385, 496, 1080
BslFI GGGAC 3 cut(s) 259, 312, 464
BslI CCNNNNNNNGG 4 cut(s) 555, 616, 661, 1085
BsmAI GTCTC 2 cut(s) 1043, 1058
BsmBI CGTCTC 1 cut(s) 1043
BsmFI GGGAC 3 cut(s) 259, 312, 464
BsnI GGCC 1 cut(s) 695
BsoBI CYCGRG 1 cut(s) 109
Bsp119I TTCGAA 1 cut(s) 1008
Bsp1286I GDGCHC 3 cut(s) 95, 127, 792
Bsp13I TCCGGA 1 cut(s) 10
Bsp143I GATC 5 cut(s) 339, 372, 675, 761, 852
Bsp19I CCATGG 1 cut(s) 86
BspANI GGCC 1 cut(s) 695
BspCNI CTCAG 2 cut(s) 415, 1197
BspDI ATCGAT 1 cut(s) 456
BspEI TCCGGA 1 cut(s) 10
BspFNI CGCG 3 cut(s) 180, 193, 1172
BspLI GGNNCC 2 cut(s) 169, 717
BspMI ACCTGC 1 cut(s) 514
BspPI GGATC 4 cut(s) 347, 367, 670, 769
BspT104I TTCGAA 1 cut(s) 1008
BspT107I GGYRCC 1 cut(s) 715
BsrFI RCCGGY 1 cut(s) 495
BsrI ACTGG 2 cut(s) 848, 1043
BssAI RCCGGY 1 cut(s) 495
BssECI CCNNGG 3 cut(s) 86, 875, 1246
BssMI GATC 5 cut(s) 339, 372, 675, 761, 852
BssT1I CCWWGG 1 cut(s) 86
Bst2UI CCWGG 1 cut(s) 377
Bst4CI ACNGT 3 cut(s) 250, 1054, 1240
Bst6I CTCTTC 2 cut(s) 1140, 1202
BstAPI GCANNNNNTGC 1 cut(s) 1195
BstBAI YACGTR 1 cut(s) 292
BstBI TTCGAA 1 cut(s) 1008
BstC8I GCNNGC 1 cut(s) 497
BstDEI CTNAG 2 cut(s) 402, 1184
BstDSI CCRYGG 2 cut(s) 86, 1246
BstF5I GGATG 2 cut(s) 30, 648
BstFNI CGCG 3 cut(s) 180, 193, 1172
BstHHI GCGC 1 cut(s) 1172
BstKTI GATC 5 cut(s) 342, 375, 678, 764, 855
BstMAI GTCTC 2 cut(s) 1043, 1058
BstMBI GATC 5 cut(s) 339, 372, 675, 761, 852
BstMWI GCNNNNNNNGC 2 cut(s) 724, 1195
BstNI CCWGG 1 cut(s) 377
BstNSI RCATGY 1 cut(s) 922
BstSCI CCNGG 2 cut(s) 375, 1078
BstSLI GKGCMC 1 cut(s) 792
BstSNI TACGTA 1 cut(s) 292
BstUI CGCG 3 cut(s) 180, 193, 1172
BstV1I GCAGC 2 cut(s) 300, 1182
BstX2I RGATCY 1 cut(s) 761
BstXI CCANNNNNNTGG 1 cut(s) 1160
BstYI RGATCY 1 cut(s) 761
Bsu15I ATCGAT 1 cut(s) 456
Bsu36I CCTNAGG 1 cut(s) 1184
BsuI GTATCC 1 cut(s) 571
BsuRI GGCC 1 cut(s) 695
BsuTUI ATCGAT 1 cut(s) 456
BtgI CCRYGG 2 cut(s) 86, 1246
BtrI CACGTC 1 cut(s) 1015
BtsCI GGATG 2 cut(s) 30, 648
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 2 cut(s) 861, 1036
BveI ACCTGC 1 cut(s) 514
Cac8I GCNNGC 1 cut(s) 497
CfoI GCGC 1 cut(s) 1172
Cfr10I RCCGGY 1 cut(s) 495
Cfr13I GGNCC 2 cut(s) 652, 1150
ClaI ATCGAT 1 cut(s) 456
CseI GACGC 2 cut(s) 64, 1125
Csp6I GTAC 4 cut(s) 382, 417, 916, 1236
CviAII CATG 4 cut(s) 87, 148, 815, 919
CviQI GTAC 4 cut(s) 382, 417, 916, 1236
DdeI CTNAG 2 cut(s) 402, 1184
DpnI GATC 5 cut(s) 341, 374, 677, 763, 854
DpnII GATC 5 cut(s) 339, 372, 675, 761, 852
EaeI YGGCCR 1 cut(s) 693
Eam1104I CTCTTC 2 cut(s) 1140, 1202
EarI CTCTTC 2 cut(s) 1140, 1202
EciI GGCGGA 1 cut(s) 645
Eco105I TACGTA 1 cut(s) 292
Eco130I CCWWGG 1 cut(s) 86
Eco24I GRGCYC 1 cut(s) 127
Eco47I GGWCC 2 cut(s) 652, 1150
Eco57I CTGAAG 2 cut(s) 152, 394
Eco81I CCTNAGG 1 cut(s) 1184
Eco88I CYCGRG 1 cut(s) 109
EcoRII CCWGG 1 cut(s) 375
EcoT14I CCWWGG 1 cut(s) 86
EcoT38I GRGCYC 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 86
Esp3I CGTCTC 1 cut(s) 1043
FaeI CATG 4 cut(s) 90, 151, 818, 922
FaiI YATR 7 cut(s) 88, 149, 603, 749, 816, 920, 1221
FalI AAGNNNNNCTT 2 cut(s) 762, 794
FaqI GGGAC 3 cut(s) 259, 312, 464
FatI CATG 4 cut(s) 86, 147, 814, 918
FauI CCCGC 2 cut(s) 185, 1105
FblI GTMKAC 2 cut(s) 27, 1017
Fnu4HI GCNGC 7 cut(s) 314, 521, 584, 719, 923, 1110, 1196
FokI GGATG 2 cut(s) 17, 655
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 7 cut(s) 314, 521, 584, 719, 923, 1110, 1196
GlaI GCGC 1 cut(s) 1171
GluI GCNGC 7 cut(s) 314, 521, 584, 719, 923, 1110, 1196
GsaI CCCAGC 2 cut(s) 167, 434
GsuI CTGGAG 2 cut(s) 577, 865
HaeIII GGCC 1 cut(s) 695
HapII CCGG 4 cut(s) 11, 385, 496, 1080
HgaI GACGC 2 cut(s) 64, 1125
HhaI GCGC 1 cut(s) 1172
Hin1II CATG 4 cut(s) 90, 151, 818, 922
Hin6I GCGC 1 cut(s) 1170
HinP1I GCGC 1 cut(s) 1170
HincII GTYRAC 2 cut(s) 28, 1018
HindII GTYRAC 2 cut(s) 28, 1018
HpaII CCGG 4 cut(s) 11, 385, 496, 1080
HphI GGTGA 1 cut(s) 850
Hpy166II GTNNAC 3 cut(s) 28, 790, 1018
Hpy188III TCNNGA 4 cut(s) 11, 244, 556, 569
Hpy8I GTNNAC 3 cut(s) 28, 790, 1018
Hpy99I CGWCG 6 cut(s) 29, 373, 487, 701, 1019, 1141
HpyAV CCTTC 3 cut(s) 137, 405, 934
HpyCH4III ACNGT 3 cut(s) 250, 1054, 1240
HpyCH4IV ACGT 3 cut(s) 291, 1014, 1128
HpyCH4V TGCA 3 cut(s) 81, 790, 1045
HpyF10VI GCNNNNNNNGC 2 cut(s) 724, 1195
HpyF3I CTNAG 2 cut(s) 402, 1184
HpySE526I ACGT 3 cut(s) 291, 1014, 1128
Hsp92II CATG 4 cut(s) 90, 151, 818, 922
HspAI GCGC 1 cut(s) 1170
Kpn2I TCCGGA 1 cut(s) 10
KroI GCCGGC 1 cut(s) 495
KroNI GCCGGC 1 cut(s) 497
Kzo9I GATC 5 cut(s) 339, 372, 675, 761, 852
LmnI GCTCC 3 cut(s) 4, 396, 944
Lsp1109I GCAGC 2 cut(s) 300, 1182
LweI GCATC 1 cut(s) 436
MaeII ACGT 3 cut(s) 291, 1014, 1128
MaeIII GTNAC 5 cut(s) 194, 440, 505, 512, 979
MalI GATC 5 cut(s) 341, 374, 677, 763, 854
MboI GATC 5 cut(s) 339, 372, 675, 761, 852
MboII GAAGA 5 cut(s) 26, 145, 574, 1157, 1219
MfeI CAATTG 1 cut(s) 1190
MflI RGATCY 1 cut(s) 761
MhlI GDGCHC 3 cut(s) 95, 127, 792
MluCI AATT 3 cut(s) 357, 550, 1190
MlyI GAGTC 4 cut(s) 230, 486, 1060, 1112
MmeI TCCRAC 6 cut(s) 722, 908, 943, 1104, 1119, 1121
MroI TCCGGA 1 cut(s) 10
MroNI GCCGGC 1 cut(s) 495
MseI TTAA 2 cut(s) 102, 708
MslI CAYNNNNRTG 3 cut(s) 179, 306, 780
MspA1I CMGCKG 2 cut(s) 925, 1109
MspI CCGG 4 cut(s) 11, 385, 496, 1080
MspR9I CCNGG 2 cut(s) 377, 1080
MunI CAATTG 1 cut(s) 1190
MvaI CCWGG 1 cut(s) 377
MvnI CGCG 3 cut(s) 180, 193, 1172
MwoI GCNNNNNNNGC 2 cut(s) 724, 1195
NaeI GCCGGC 1 cut(s) 497
NciI CCSGG 1 cut(s) 1080
NcoI CCATGG 1 cut(s) 86
NdeII GATC 5 cut(s) 339, 372, 675, 761, 852
NgoMIV GCCGGC 1 cut(s) 495
NlaIII CATG 4 cut(s) 90, 151, 818, 922
NlaIV GGNNCC 2 cut(s) 169, 717
NmuCI GTSAC 1 cut(s) 979
NspI RCATGY 1 cut(s) 922
NspV TTCGAA 1 cut(s) 1008
OliI CACNNNNGTG 2 cut(s) 179, 780
PcsI WCGNNNNNNNCGW 3 cut(s) 249, 693, 1014
PdiI GCCGGC 1 cut(s) 497
PfeI GAWTC 8 cut(s) 66, 565, 659, 684, 880, 905, 1033, 1093
PflFI GACNNNGTC 1 cut(s) 1102
PflMI CCANNNNNTGG 1 cut(s) 555
PfoI TCCNGGA 1 cut(s) 375
PkrI GCNGC 7 cut(s) 315, 522, 585, 720, 924, 1111, 1197
PleI GAGTC 4 cut(s) 229, 485, 1060, 1111
PpsI GAGTC 4 cut(s) 229, 485, 1060, 1111
Ppu21I YACGTR 1 cut(s) 292
Psp6I CCWGG 1 cut(s) 375
PspFI CCCAGC 2 cut(s) 163, 430
PspGI CCWGG 1 cut(s) 375
PspN4I GGNNCC 2 cut(s) 169, 717
PspPI GGNCC 2 cut(s) 652, 1150
PsuI RGATCY 1 cut(s) 761
PsyI GACNNNGTC 1 cut(s) 1102
RsaI GTAC 4 cut(s) 383, 418, 917, 1237
RsaNI GTAC 4 cut(s) 382, 417, 916, 1236
RseI CAYNNNNRTG 3 cut(s) 179, 306, 780
SalI GTCGAC 2 cut(s) 26, 1016
SaqAI TTAA 2 cut(s) 102, 708
SatI GCNGC 7 cut(s) 314, 521, 584, 719, 923, 1110, 1196
Sau3AI GATC 5 cut(s) 339, 372, 675, 761, 852
Sau96I GGNCC 2 cut(s) 652, 1150
SchI GAGTC 4 cut(s) 230, 486, 1060, 1112
ScrFI CCNGG 2 cut(s) 377, 1080
SduI GDGCHC 3 cut(s) 95, 127, 792
SfaNI GCATC 1 cut(s) 436
SfuI TTCGAA 1 cut(s) 1008
SinI GGWCC 2 cut(s) 652, 1150
SmiMI CAYNNNNRTG 3 cut(s) 179, 306, 780
SnaBI TACGTA 1 cut(s) 292
Sse9I AATT 3 cut(s) 357, 550, 1190
StyD4I CCNGG 2 cut(s) 375, 1078
StyI CCWWGG 1 cut(s) 86
TaaI ACNGT 3 cut(s) 250, 1054, 1240
TaiI ACGT 3 cut(s) 294, 1017, 1131
TasI AATT 3 cut(s) 357, 550, 1190
TauI GCSGC 5 cut(s) 523, 586, 721, 925, 1112
TfiI GAWTC 8 cut(s) 66, 565, 659, 684, 880, 905, 1033, 1093
Tru1I TTAA 2 cut(s) 102, 708
Tru9I TTAA 2 cut(s) 102, 708
TscAI CASTG 2 cut(s) 868, 1043
TseFI GTSAC 1 cut(s) 979
TseI GCWGC 2 cut(s) 313, 1195
Tsp45I GTSAC 1 cut(s) 979
TspDTI ATGAA 3 cut(s) 583, 803, 1208
TspGWI ACGGA 1 cut(s) 632
TspRI CASTG 2 cut(s) 868, 1043
Tth111I GACNNNGTC 1 cut(s) 1102
Van91I CCANNNNNTGG 1 cut(s) 555
VneI GTGCAC 1 cut(s) 788
VpaK11BI GGWCC 2 cut(s) 652, 1150
XceI RCATGY 1 cut(s) 922
XmiI GTMKAC 2 cut(s) 27, 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.