pycom01g18120

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0016396
Physical Location & Seq
Reverse (-)
17237230 .. 17238606
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g18120.1

Sequence Viewer

Length: 1377 bp
ATGGGCACCTCGCCGCCACTCCACCCAACCCAACAAGAAAAACACCCAACCCCAACCACCGCCAAACACCAGCCGCCGCCGCCGCTAAACCCAAACCCAATGGAGCTTCCGGAAGAACTCATCGTCGACATCCTCAAACGGCTTCCCCCCAAATCCCTCGTCCGATTCAGGTGCGTCTGCAAACCATGGTGCTCTCTGATTAAAAGCCCGAGTTTCGTCCAAGCCCTTCTGAAGAAAAACCGAGCCATGTTCCAATCCGAGCTGGGAACCAGCACCCGTGTGATTTTGTCGCGTTACTGCGACACCCTTTTGTCGATGGAGTCCGAAAACAACAGTGTTGTCGGGACTGTCGAGCTTGACTTCGCTCTGGTTCGGAACTTGCCGTATTACGTAAAGGGACACTCCGATGGGCTGCTCTGTGTGGTTATCAATGATGGGATCGAGGGTGTGGCTGTAATTTACAACCCGACGATCCAGGAGTACCGGAAGCTCCCTTCTCCTCAGAATTTCAGAAGTACGAGGGAGGTGCTGGGTTTGGGTTACGATGCCTCAATCGATGATTACAAGGTTGTTCGAGTCCCCTCGAACTATTGCCGGCTGAAAGTTGCGGGTTACAAGCCGCAAGTCGAGGTATTGGAACTAAAAACCAATTTCTGGAGGAAGATTCCAGATGAGGATACGCCGCCGTTTTTCATTGAGCATATTTTCCAATCAACGGAAGTGAATGGCGGGTTGTATTGGATGGCAGAGGACCACAATTCGGGAAGGTGCCTGATCCTTCGATTCGATTTGGCCGAGGAGAAGTTTAAGGTGGTGCCGCCACCGCCAGATGAGAGTGGTCGGAGTATAGCGTGGATTGGATCTTTGAAAGACCACCTTTGTGTTGTGCACACTCGGAGGTTGAGTGATGTTCATGTTTGGGGAACAAAAGATGACAAGAACTGGAGTAAGATCATCACCACGTCGAAGTTTCCGAGGATTCCGACCAAAGACCCGTTCCTTGATTCGTTTCGGTACATGCCGCTGTCTTTTACCAAGAAGGGAGCTGTGCTAATGAGCGTTGGAGGGGAGAGGTTTGTGACATTAGATACAAAAGACAATACATTCGAACACGTCGACATCAAAGGTGCGAATCATTGGCTGCAGGAGACGGTGTATTGCGAGACTCTTGTTTCGCCCGGTGGTGGTGTGAATCCGACAGAGTCGGCGGCGGGGTTGGGGTCGGATGTTGGTGCGTCGGAGGAAGAGGAAGAGGACCAATCAGACGGGGAAAGCGCGAGTAATAGCCTCAGGCAATTGCTGCTTGGTTTGAAGAGGGAAGTTCATAACTTGTTGGCTTGTACCGTTAGCCACAGGAATATCAGAGATGCGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

459

Amino Acids

51.44

Weight (kDa)

6.15

Isoelectric Point (pI)

46.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 35 - 72 5.7e-12 F-box domain
F-box-like PF12937 35 - 72 1.6e-09 F-box-like
FBA_3 PF08268 129 - 379 4.7e-21 F-box associated beta propeller domain
FBA_1 PF07734 133 - 391 7.8e-25 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016396)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g26000
malus_domestica MD01G1168200.v1.1
prunus_persica Prupe.2G264700_v2.0.a1
pyrus_communis pycom01g18120
rosa_chinensis RchiOBHm_Chr1g0372381
rosa_laevigata RLG00000026902
rosa_multiflora Rmu_sc0001755.1_g000005 Rmu_sc0032499.1_g000001
rosa_roxburghii Rroxscaffold_4G00284990
rosa_rugosa Rorug01G0371400
rosa_samantha Rh1AG380100 Rh1BG343600 Rh1CG357100 Rh1DG374800
rosa_wichuraiana Rw1G033410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 5, 768, 814
AccB7I CCANNNNNTGG 1 cut(s) 654
AccI GTMKAC 2 cut(s) 126, 1116
AccII CGCG 2 cut(s) 292, 1277
AccIII TCCGGA 1 cut(s) 109
AclWI GGATC 4 cut(s) 446, 466, 769, 868
AcoI YGGCCR 1 cut(s) 792
AcsI RAATTY 1 cut(s) 505
AcuI CTGAAG 1 cut(s) 251
AfaI GTAC 4 cut(s) 482, 517, 1016, 1342
AfiI CCNNNNNNNGG 4 cut(s) 654, 715, 760, 1184
AflIII ACRYGT 1 cut(s) 1111
AgsI TTSAA 2 cut(s) 868, 1312
AjiI CACGTC 2 cut(s) 963, 1114
AjnI CCWGG 1 cut(s) 474
AjuI GAANNNNNNNTTGG 2 cut(s) 980, 1012
AleI CACNNNNGTG 2 cut(s) 278, 879
AluBI AGCT 5 cut(s) 106, 262, 355, 490, 1046
AluI AGCT 5 cut(s) 106, 262, 355, 490, 1046
Alw21I GWGCWC 2 cut(s) 194, 891
Alw26I GTCTC 2 cut(s) 1142, 1157
Alw44I GTGCAC 1 cut(s) 887
AlwI GGATC 4 cut(s) 446, 466, 769, 868
Ama87I CYCGRG 1 cut(s) 208
Aor13HI TCCGGA 1 cut(s) 109
AoxI GGCC 1 cut(s) 792
ApaLI GTGCAC 1 cut(s) 887
ApeKI GCWGC 3 cut(s) 412, 1141, 1300
ApoI RAATTY 1 cut(s) 505
ArsI GACNNNNNNTTYG 6 cut(s) 144, 176, 1088, 1120, 1156, 1188
AspLEI GCGC 1 cut(s) 1277
AspS9I GGNCC 2 cut(s) 751, 1255
AsuC2I CCSGG 1 cut(s) 1179
AsuHPI GGTGA 1 cut(s) 949
AsuII TTCGAA 1 cut(s) 1107
AvaI CYCGRG 1 cut(s) 208
AvaII GGWCC 2 cut(s) 751, 1255
AxyI CCTNAGG 1 cut(s) 1289
BaeGI GKGCMC 2 cut(s) 8, 891
BanI GGYRCC 3 cut(s) 5, 768, 814
Bbv12I GWGCWC 2 cut(s) 194, 891
BbvI GCAGC 3 cut(s) 399, 1128, 1287
BccI CCATC 4 cut(s) 310, 401, 428, 736
BceAI ACGGC 3 cut(s) 155, 367, 670
BcgI CGANNNNNNTGC 4 cut(s) 153, 187, 508, 542
BciT130I CCWGG 1 cut(s) 476
BciVI GTATCC 1 cut(s) 670
BcnI CCSGG 1 cut(s) 1179
BcoDI GTCTC 2 cut(s) 1142, 1157
BfmI CTRYAG 1 cut(s) 1142
BfuI GTATCC 1 cut(s) 670
Bme1390I CCNGG 2 cut(s) 476, 1179
Bme18I GGWCC 2 cut(s) 751, 1255
BmeT110I CYCGRG 1 cut(s) 208
BmgBI CACGTC 2 cut(s) 963, 1114
BmgT120I GGNCC 2 cut(s) 751, 1255
BmiI GGNNCC 4 cut(s) 7, 268, 770, 816
BmrFI CCNGG 2 cut(s) 476, 1179
BmsI GCATC 2 cut(s) 535, 1357
BpmI CTGGAG 2 cut(s) 676, 964
Bpu14I TTCGAA 1 cut(s) 1107
BpuMI CCSGG 1 cut(s) 1179
Bsa29I ATCGAT 1 cut(s) 555
BsaAI YACGTR 1 cut(s) 391
BsaJI CCNNGG 3 cut(s) 185, 795, 974
BsaWI WCCGGW 2 cut(s) 109, 483
BsaXI ACNNNNNCTCC 4 cut(s) 889, 919, 1139, 1169
Bsc4I CCNNNNNNNGG 4 cut(s) 654, 715, 760, 1184
Bse118I RCCGGY 1 cut(s) 594
Bse1I ACTGG 1 cut(s) 947
Bse21I CCTNAGG 1 cut(s) 1289
BseAI TCCGGA 1 cut(s) 109
BseBI CCWGG 1 cut(s) 476
BseCI ATCGAT 1 cut(s) 555
BseDI CCNNGG 3 cut(s) 185, 795, 974
BseGI GGATG 3 cut(s) 129, 747, 1231
BseLI CCNNNNNNNGG 4 cut(s) 654, 715, 760, 1184
BseMII CTCAG 2 cut(s) 515, 1303
BseNI ACTGG 1 cut(s) 947
BseRI GAGGAG 2 cut(s) 489, 812
BseSI GKGCMC 2 cut(s) 8, 891
BseXI GCAGC 3 cut(s) 399, 1128, 1287
BseYI CCCAGC 2 cut(s) 262, 529
Bsh1236I CGCG 2 cut(s) 292, 1277
BshFI GGCC 1 cut(s) 794
BshNI GGYRCC 3 cut(s) 5, 768, 814
BshVI ATCGAT 1 cut(s) 555
BsiHKAI GWGCWC 2 cut(s) 194, 891
BsiHKCI CYCGRG 1 cut(s) 208
BsiSI CCGG 4 cut(s) 110, 484, 595, 1179
BslFI GGGAC 3 cut(s) 358, 411, 563
BslI CCNNNNNNNGG 4 cut(s) 654, 715, 760, 1184
BsmAI GTCTC 2 cut(s) 1142, 1157
BsmBI CGTCTC 1 cut(s) 1142
BsmFI GGGAC 3 cut(s) 358, 411, 563
BsnI GGCC 1 cut(s) 794
BsoBI CYCGRG 1 cut(s) 208
Bsp119I TTCGAA 1 cut(s) 1107
Bsp1286I GDGCHC 3 cut(s) 8, 194, 891
Bsp13I TCCGGA 1 cut(s) 109
Bsp143I GATC 5 cut(s) 438, 471, 774, 860, 951
Bsp19I CCATGG 1 cut(s) 185
BspANI GGCC 1 cut(s) 794
BspCNI CTCAG 2 cut(s) 514, 1302
BspDI ATCGAT 1 cut(s) 555
BspEI TCCGGA 1 cut(s) 109
BspFNI CGCG 2 cut(s) 292, 1277
BspLI GGNNCC 4 cut(s) 7, 268, 770, 816
BspMAI CTGCAG 1 cut(s) 1146
BspPI GGATC 4 cut(s) 446, 466, 769, 868
BspT104I TTCGAA 1 cut(s) 1107
BspT107I GGYRCC 3 cut(s) 5, 768, 814
BsrFI RCCGGY 1 cut(s) 594
BsrI ACTGG 1 cut(s) 947
BssAI RCCGGY 1 cut(s) 594
BssECI CCNNGG 3 cut(s) 185, 795, 974
BssMI GATC 5 cut(s) 438, 471, 774, 860, 951
BssT1I CCWWGG 1 cut(s) 185
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 4 cut(s) 335, 349, 1153, 1345
Bst6I CTCTTC 3 cut(s) 1239, 1245, 1307
BstAPI GCANNNNNTGC 1 cut(s) 1300
BstBAI YACGTR 1 cut(s) 391
BstBI TTCGAA 1 cut(s) 1107
BstC8I GCNNGC 1 cut(s) 596
BstDEI CTNAG 2 cut(s) 501, 1289
BstDSI CCRYGG 1 cut(s) 185
BstF5I GGATG 3 cut(s) 129, 747, 1231
BstFNI CGCG 2 cut(s) 292, 1277
BstHHI GCGC 1 cut(s) 1277
BstKTI GATC 5 cut(s) 441, 474, 777, 863, 954
BstMAI GTCTC 2 cut(s) 1142, 1157
BstMBI GATC 5 cut(s) 438, 471, 774, 860, 951
BstMWI GCNNNNNNNGC 4 cut(s) 79, 82, 823, 1300
BstNI CCWGG 1 cut(s) 476
BstNSI RCATGY 1 cut(s) 1021
BstSCI CCNGG 2 cut(s) 474, 1177
BstSFI CTRYAG 1 cut(s) 1142
BstSLI GKGCMC 2 cut(s) 8, 891
BstSNI TACGTA 1 cut(s) 391
BstUI CGCG 2 cut(s) 292, 1277
BstV1I GCAGC 3 cut(s) 399, 1128, 1287
BstX2I RGATCY 1 cut(s) 860
BstYI RGATCY 1 cut(s) 860
Bsu15I ATCGAT 1 cut(s) 555
Bsu36I CCTNAGG 1 cut(s) 1289
BsuI GTATCC 1 cut(s) 670
BsuRI GGCC 1 cut(s) 794
BsuTUI ATCGAT 1 cut(s) 555
BtgI CCRYGG 1 cut(s) 185
BtrI CACGTC 2 cut(s) 963, 1114
BtsCI GGATG 3 cut(s) 129, 747, 1231
BtsIMutI CAGTG 1 cut(s) 340
Cac8I GCNNGC 1 cut(s) 596
CfoI GCGC 1 cut(s) 1277
Cfr10I RCCGGY 1 cut(s) 594
Cfr13I GGNCC 2 cut(s) 751, 1255
ClaI ATCGAT 1 cut(s) 555
CseI GACGC 2 cut(s) 163, 1224
Csp6I GTAC 4 cut(s) 481, 516, 1015, 1341
CviAII CATG 4 cut(s) 186, 247, 914, 1018
CviQI GTAC 4 cut(s) 481, 516, 1015, 1341
DdeI CTNAG 2 cut(s) 501, 1289
DpnI GATC 5 cut(s) 440, 473, 776, 862, 953
DpnII GATC 5 cut(s) 438, 471, 774, 860, 951
EaeI YGGCCR 1 cut(s) 792
Eam1104I CTCTTC 3 cut(s) 1239, 1245, 1307
EarI CTCTTC 3 cut(s) 1239, 1245, 1307
Eco105I TACGTA 1 cut(s) 391
Eco130I CCWWGG 1 cut(s) 185
Eco47I GGWCC 2 cut(s) 751, 1255
Eco57I CTGAAG 1 cut(s) 251
Eco81I CCTNAGG 1 cut(s) 1289
Eco88I CYCGRG 1 cut(s) 208
EcoRII CCWGG 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 185
ErhI CCWWGG 1 cut(s) 185
Esp3I CGTCTC 1 cut(s) 1142
FaeI CATG 4 cut(s) 189, 250, 917, 1021
FaiI YATR 7 cut(s) 187, 248, 702, 848, 915, 1019, 1326
FalI AAGNNNNNCTT 2 cut(s) 861, 893
FaqI GGGAC 3 cut(s) 358, 411, 563
FatI CATG 4 cut(s) 185, 246, 913, 1017
FauI CCCGC 3 cut(s) 601, 722, 1204
FblI GTMKAC 2 cut(s) 126, 1116
FokI GGATG 3 cut(s) 116, 754, 1238
GlaI GCGC 1 cut(s) 1276
GsaI CCCAGC 2 cut(s) 266, 533
GsuI CTGGAG 2 cut(s) 676, 964
HaeIII GGCC 1 cut(s) 794
HapII CCGG 4 cut(s) 110, 484, 595, 1179
HgaI GACGC 2 cut(s) 163, 1224
HhaI GCGC 1 cut(s) 1277
Hin1II CATG 4 cut(s) 189, 250, 917, 1021
Hin6I GCGC 1 cut(s) 1275
HinP1I GCGC 1 cut(s) 1275
HincII GTYRAC 2 cut(s) 127, 1117
HindII GTYRAC 2 cut(s) 127, 1117
HpaII CCGG 4 cut(s) 110, 484, 595, 1179
HphI GGTGA 1 cut(s) 949
Hpy166II GTNNAC 3 cut(s) 127, 889, 1117
Hpy188III TCNNGA 5 cut(s) 110, 343, 655, 668, 762
Hpy8I GTNNAC 3 cut(s) 127, 889, 1117
Hpy99I CGWCG 5 cut(s) 128, 472, 967, 1118, 1240
HpyAV CCTTC 5 cut(s) 236, 504, 759, 788, 1033
HpyCH4III ACNGT 4 cut(s) 335, 349, 1153, 1345
HpyCH4IV ACGT 3 cut(s) 390, 962, 1113
HpyCH4V TGCA 3 cut(s) 180, 889, 1144
HpyF10VI GCNNNNNNNGC 4 cut(s) 79, 82, 823, 1300
HpyF3I CTNAG 2 cut(s) 501, 1289
HpySE526I ACGT 3 cut(s) 390, 962, 1113
Hsp92II CATG 4 cut(s) 189, 250, 917, 1021
HspAI GCGC 1 cut(s) 1275
Kpn2I TCCGGA 1 cut(s) 109
KroI GCCGGC 1 cut(s) 594
KroNI GCCGGC 1 cut(s) 596
Kzo9I GATC 5 cut(s) 438, 471, 774, 860, 951
LmnI GCTCC 3 cut(s) 103, 495, 1043
Lsp1109I GCAGC 3 cut(s) 399, 1128, 1287
LweI GCATC 2 cut(s) 535, 1357
MaeII ACGT 3 cut(s) 390, 962, 1113
MaeIII GTNAC 4 cut(s) 293, 539, 611, 1078
MalI GATC 5 cut(s) 440, 473, 776, 862, 953
MboI GATC 5 cut(s) 438, 471, 774, 860, 951
MboII GAAGA 6 cut(s) 125, 244, 673, 1256, 1262, 1324
MfeI CAATTG 1 cut(s) 1295
MflI RGATCY 1 cut(s) 860
MhlI GDGCHC 3 cut(s) 8, 194, 891
MluCI AATT 5 cut(s) 456, 505, 649, 757, 1295
MlyI GAGTC 4 cut(s) 329, 585, 1159, 1211
MmeI TCCRAC 6 cut(s) 821, 1007, 1042, 1203, 1218, 1220
MroI TCCGGA 1 cut(s) 109
MroNI GCCGGC 1 cut(s) 594
MseI TTAA 2 cut(s) 201, 807
MslI CAYNNNNRTG 3 cut(s) 278, 405, 879
MspA1I CMGCKG 1 cut(s) 1024
MspI CCGG 4 cut(s) 110, 484, 595, 1179
MspR9I CCNGG 2 cut(s) 476, 1179
MunI CAATTG 1 cut(s) 1295
MvaI CCWGG 1 cut(s) 476
MvnI CGCG 2 cut(s) 292, 1277
MwoI GCNNNNNNNGC 4 cut(s) 79, 82, 823, 1300
NaeI GCCGGC 1 cut(s) 596
NciI CCSGG 1 cut(s) 1179
NcoI CCATGG 1 cut(s) 185
NdeII GATC 5 cut(s) 438, 471, 774, 860, 951
NgoMIV GCCGGC 1 cut(s) 594
NlaIII CATG 4 cut(s) 189, 250, 917, 1021
NlaIV GGNNCC 4 cut(s) 7, 268, 770, 816
NmeAIII GCCGAG 1 cut(s) 820
NmuCI GTSAC 1 cut(s) 1078
NspI RCATGY 1 cut(s) 1021
NspV TTCGAA 1 cut(s) 1107
OliI CACNNNNGTG 2 cut(s) 278, 879
PcsI WCGNNNNNNNCGW 4 cut(s) 348, 792, 971, 1113
PdiI GCCGGC 1 cut(s) 596
PfeI GAWTC 7 cut(s) 165, 664, 783, 979, 1004, 1132, 1192
PflFI GACNNNGTC 1 cut(s) 1201
PflMI CCANNNNNTGG 1 cut(s) 654
PfoI TCCNGGA 1 cut(s) 474
PleI GAGTC 4 cut(s) 328, 584, 1159, 1210
PpsI GAGTC 4 cut(s) 328, 584, 1159, 1210
Ppu21I YACGTR 1 cut(s) 391
Psp6I CCWGG 1 cut(s) 474
PspFI CCCAGC 2 cut(s) 262, 529
PspGI CCWGG 1 cut(s) 474
PspN4I GGNNCC 4 cut(s) 7, 268, 770, 816
PspPI GGNCC 2 cut(s) 751, 1255
PstI CTGCAG 1 cut(s) 1146
PsuI RGATCY 1 cut(s) 860
PsyI GACNNNGTC 1 cut(s) 1201
RsaI GTAC 4 cut(s) 482, 517, 1016, 1342
RsaNI GTAC 4 cut(s) 481, 516, 1015, 1341
RseI CAYNNNNRTG 3 cut(s) 278, 405, 879
SalI GTCGAC 2 cut(s) 125, 1115
SaqAI TTAA 2 cut(s) 201, 807
Sau3AI GATC 5 cut(s) 438, 471, 774, 860, 951
Sau96I GGNCC 2 cut(s) 751, 1255
SchI GAGTC 4 cut(s) 329, 585, 1159, 1211
ScrFI CCNGG 2 cut(s) 476, 1179
SduI GDGCHC 3 cut(s) 8, 194, 891
SfaNI GCATC 2 cut(s) 535, 1357
SfcI CTRYAG 1 cut(s) 1142
SfuI TTCGAA 1 cut(s) 1107
SinI GGWCC 2 cut(s) 751, 1255
SmiMI CAYNNNNRTG 3 cut(s) 278, 405, 879
SnaBI TACGTA 1 cut(s) 391
Sse9I AATT 5 cut(s) 456, 505, 649, 757, 1295
StyD4I CCNGG 2 cut(s) 474, 1177
StyI CCWWGG 1 cut(s) 185
TaaI ACNGT 4 cut(s) 335, 349, 1153, 1345
TaiI ACGT 3 cut(s) 393, 965, 1116
TasI AATT 5 cut(s) 456, 505, 649, 757, 1295
TfiI GAWTC 7 cut(s) 165, 664, 783, 979, 1004, 1132, 1192
Tru1I TTAA 2 cut(s) 201, 807
Tru9I TTAA 2 cut(s) 201, 807
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 1 cut(s) 1078
TseI GCWGC 3 cut(s) 412, 1141, 1300
Tsp45I GTSAC 1 cut(s) 1078
TspDTI ATGAA 3 cut(s) 682, 902, 1313
TspGWI ACGGA 1 cut(s) 731
TspRI CASTG 1 cut(s) 340
Tth111I GACNNNGTC 1 cut(s) 1201
Van91I CCANNNNNTGG 1 cut(s) 654
VneI GTGCAC 1 cut(s) 887
VpaK11BI GGWCC 2 cut(s) 751, 1255
XapI RAATTY 1 cut(s) 505
XceI RCATGY 1 cut(s) 1021
XmiI GTMKAC 2 cut(s) 126, 1116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.