Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0015808

Physical Location & Seq

Genomic Coordinates Forward (+)

27726352 .. 27729626

Gene Structure 3275 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1176600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 609 bp

ATGAATCCTGAATATGATTACTTGTTTAAACTTTTGCTGATTGGAGACTCTGGGGTTGGAAAATCATGTCTTCTTCTGAGGTTTGCTGACGATTCATATCTGGACAGCTACATTAGCACAATAGGAGTTGATTTTAAAATTCGCACAGTAGAGCAGGATGAGAAGACCATTAAACTTCAAATTTGGGACACTGCTGGACAAGAACGTTTTAGGACAATTACTAGTAGCTACTACCGTGGGGCACATGGAATTATAATAGTTTATGATGTGACGGACCAAGAGAGCTTCAATAATGTTAAGCAATGGTTAAATGAAATTGACCGTTACGCTAGCGAGAATGTAAACAAGCTTCTTGTAGGCAACAAGGCTGATCTCACTGCTAACAAAGTTGTGTCTTATGAAACAGCTAAGGCATTTGCGGATGAGATTGGGATCCCTTTCATGGAGACTAGTGCAAAAAATGCTACCAATGTGGAGCAGGCTTTCATGGCAATGGCTGCTGAAATCAAGAATAGGATGGCAAGTCAACCAATGAACAATGCAAGGCCGCCAACAGTGCAGATCCGCGGACAACCTGTGAACCAGAAATCTGGCTGCTGCTCATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000139 GO:0000902 GO:0000904 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005789 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006888 GO:0007275 GO:0008150 GO:0009506 GO:0009555 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0022414 GO:0030054 GO:0030154 GO:0031090 GO:0031984 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0046907 GO:0048193 GO:0048229 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051704 GO:0055044 GO:0060560 GO:0071840 GO:0071944 GO:0098588 GO:0098791 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko05134 map05134

Pfam Domains

Protein Families

Ras

Protein Analysis

203

Amino Acids

22.66

Weight (kDa)

5.3

Isoelectric Point (pI)

30.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	9 - 129	1.3e-16	ADP-ribosylation factor family
Ras	PF00071	10 - 170	2.4e-68	Ras family
Roc	PF08477	10 - 124	7.2e-37	Ras of Complex, Roc, domain of DAPkinase
GTP_EFTU	PF00009	45 - 166	8e-06	Elongation factor Tu GTP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0015808)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02130
fragaria_vesca	FvH4_7g26840
malus_domestica	MD01G1176600.v1.1 MD04G1050400.v1.1 MD07G1247800.v1.1
prunus_persica	Prupe.2G271800_v2.0.a1 Prupe.2G271800_v2.0.a1
pyrus_communis	pycom01g18730
rosa_chinensis	RchiOBHm_Chr1g0373531
rosa_laevigata	RLG00000026818
rosa_roxburghii	Rroxscaffold_4G00284170
rosa_rugosa	Rorug01G0377900 Rorug01G0378000
rosa_samantha	Rh1CG364400
rosa_wichuraiana	Rw1G034800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	254
AccII	CGCG	1 cut(s)	567
AciI	CCGC	4 cut(s)	419, 548, 565, 567
AclI	AACGTT	1 cut(s)	205
AclWI	GGATC	3 cut(s)	427, 440, 556
AcsI	RAATTY	2 cut(s)	138, 180
AfiI	CCNNNNNNNGG	1 cut(s)	442
AgsI	TTSAA	2 cut(s)	179, 289
AhlI	ACTAGT	2 cut(s)	221, 449
AluBI	AGCT	5 cut(s)	108, 228, 285, 349, 407
AluI	AGCT	5 cut(s)	108, 228, 285, 349, 407
Alw26I	GTCTC	2 cut(s)	39, 440
AlwI	GGATC	3 cut(s)	427, 440, 556
AoxI	GGCC	1 cut(s)	545
ApeKI	GCWGC	3 cut(s)	497, 594, 597
ApoI	RAATTY	2 cut(s)	138, 180
AspS9I	GGNCC	1 cut(s)	274
AsuNHI	GCTAGC	1 cut(s)	329
AvaII	GGWCC	1 cut(s)	274
BaeGI	GKGCMC	1 cut(s)	244
BamHI	GGATCC	1 cut(s)	432
BarI	GAAGNNNNNNTAC	2 cut(s)	333, 365
BbsI	GAAGAC	2 cut(s)	62, 170
BbvI	GCAGC	3 cut(s)	484, 581, 584
BccI	CCATC	1 cut(s)	511
BcoDI	GTCTC	2 cut(s)	39, 440
BcuI	ACTAGT	2 cut(s)	221, 449
BfaI	CTAG	3 cut(s)	222, 330, 450
BisI	GCNGC	4 cut(s)	498, 548, 595, 598
BlsI	GCNGC	4 cut(s)	499, 549, 596, 599
Bme18I	GGWCC	1 cut(s)	274
BmgT120I	GGNCC	1 cut(s)	274
BmiI	GGNNCC	1 cut(s)	434
BmtI	GCTAGC	1 cut(s)	333
BpiI	GAAGAC	2 cut(s)	62, 170
Bpu10I	CCTNAGC	1 cut(s)	408
BsaBI	GATNNNNATC	2 cut(s)	96, 431
BsaJI	CCNNGG	2 cut(s)	235, 565
Bsc4I	CCNNNNNNNGG	1 cut(s)	442
Bse3DI	GCAATG	2 cut(s)	308, 498
Bse8I	GATNNNNATC	2 cut(s)	96, 431
BseDI	CCNNGG	2 cut(s)	235, 565
BseGI	GGATG	3 cut(s)	163, 427, 522
BseJI	GATNNNNATC	2 cut(s)	96, 431
BseLI	CCNNNNNNNGG	1 cut(s)	442
BseMI	GCAATG	2 cut(s)	308, 498
BseMII	CTCAG	1 cut(s)	68
BseSI	GKGCMC	1 cut(s)	244
BseXI	GCAGC	3 cut(s)	484, 581, 584
BsgI	GTGCAG	1 cut(s)	578
Bsh1236I	CGCG	1 cut(s)	567
BshFI	GGCC	1 cut(s)	547
BslFI	GGGAC	1 cut(s)	200
BslI	CCNNNNNNNGG	1 cut(s)	442
BsmAI	GTCTC	2 cut(s)	39, 440
BsmFI	GGGAC	1 cut(s)	200
BsnI	GGCC	1 cut(s)	547
Bsp1286I	GDGCHC	1 cut(s)	244
Bsp143I	GATC	3 cut(s)	370, 432, 561
BspACI	CCGC	4 cut(s)	419, 548, 565, 567
BspANI	GGCC	1 cut(s)	547
BspCNI	CTCAG	1 cut(s)	69
BspFNI	CGCG	1 cut(s)	567
BspLI	GGNNCC	1 cut(s)	434
BspOI	GCTAGC	1 cut(s)	333
BspPI	GGATC	3 cut(s)	427, 440, 556
BsrDI	GCAATG	2 cut(s)	308, 498
BssECI	CCNNGG	2 cut(s)	235, 565
BssMI	GATC	3 cut(s)	370, 432, 561
Bst4CI	ACNGT	4 cut(s)	148, 236, 323, 556
BstAPI	GCANNNNNTGC	2 cut(s)	461, 497
BstC8I	GCNNGC	2 cut(s)	331, 480
BstDEI	CTNAG	3 cut(s)	77, 408, 606
BstDSI	CCRYGG	2 cut(s)	235, 565
BstF5I	GGATG	3 cut(s)	163, 427, 522
BstFNI	CGCG	1 cut(s)	567
BstKTI	GATC	3 cut(s)	373, 435, 564
BstMAI	GTCTC	2 cut(s)	39, 440
BstMBI	GATC	3 cut(s)	370, 432, 561
BstMWI	GCNNNNNNNGC	5 cut(s)	114, 461, 488, 497, 556
BstSLI	GKGCMC	1 cut(s)	244
BstUI	CGCG	1 cut(s)	567
BstV1I	GCAGC	3 cut(s)	484, 581, 584
BstV2I	GAAGAC	2 cut(s)	62, 170
BstX2I	RGATCY	2 cut(s)	432, 561
BstXI	CCANNNNNNTGG	1 cut(s)	590
BstYI	RGATCY	2 cut(s)	432, 561
BsuRI	GGCC	1 cut(s)	547
BtgI	CCRYGG	2 cut(s)	235, 565
BtsCI	GGATG	3 cut(s)	163, 427, 522
BtsI	GCAGTG	2 cut(s)	189, 375
BtsIMutI	CAGTG	3 cut(s)	189, 375, 561
Cac8I	GCNNGC	2 cut(s)	331, 480
Cfr13I	GGNCC	1 cut(s)	274
Cfr42I	CCGCGG	1 cut(s)	568
CviAII	CATG	4 cut(s)	66, 245, 442, 487
DdeI	CTNAG	3 cut(s)	77, 408, 606
DpnI	GATC	3 cut(s)	372, 434, 563
DpnII	GATC	3 cut(s)	370, 432, 561
DraI	TTTAAA	2 cut(s)	28, 136
Eco47I	GGWCC	1 cut(s)	274
FaeI	CATG	4 cut(s)	69, 248, 445, 490
FaiI	YATR	9 cut(s)	15, 67, 97, 246, 254, 264, 399, 443, 488
FaqI	GGGAC	1 cut(s)	200
FatI	CATG	4 cut(s)	65, 244, 441, 486
Fnu4HI	GCNGC	4 cut(s)	498, 548, 595, 598
FokI	GGATG	3 cut(s)	170, 434, 529
Fsp4HI	GCNGC	4 cut(s)	498, 548, 595, 598
FspBI	CTAG	3 cut(s)	222, 330, 450
GluI	GCNGC	4 cut(s)	498, 548, 595, 598
HaeIII	GGCC	1 cut(s)	547
Hin1II	CATG	4 cut(s)	69, 248, 445, 490
HincII	GTYRAC	1 cut(s)	527
HindII	GTYRAC	1 cut(s)	527
HindIII	AAGCTT	1 cut(s)	347
HinfI	GANTC	3 cut(s)	4, 47, 92
Hpy166II	GTNNAC	3 cut(s)	343, 527, 580
Hpy188I	TCNGA	1 cut(s)	78
Hpy188III	TCNNGA	3 cut(s)	8, 101, 508
Hpy8I	GTNNAC	3 cut(s)	343, 527, 580
HpyCH4III	ACNGT	4 cut(s)	148, 236, 323, 556
HpyCH4IV	ACGT	1 cut(s)	205
HpyCH4V	TGCA	3 cut(s)	455, 542, 559
HpyF10VI	GCNNNNNNNGC	5 cut(s)	114, 461, 488, 497, 556
HpyF3I	CTNAG	3 cut(s)	77, 408, 606
HpySE526I	ACGT	1 cut(s)	205
Hsp92II	CATG	4 cut(s)	69, 248, 445, 490
KspI	CCGCGG	1 cut(s)	568
Kzo9I	GATC	3 cut(s)	370, 432, 561
LmnI	GCTCC	1 cut(s)	475
LpnPI	CCDG	9 cut(s)	21, 36, 86, 140, 180, 464, 576, 588, 596
Lsp1109I	GCAGC	3 cut(s)	484, 581, 584
MaeI	CTAG	3 cut(s)	222, 330, 450
MaeII	ACGT	1 cut(s)	205
MaeIII	GTNAC	2 cut(s)	268, 323
MalI	GATC	3 cut(s)	372, 434, 563
MboI	GATC	3 cut(s)	370, 432, 561
MboII	GAAGA	3 cut(s)	62, 65, 175
MflI	RGATCY	2 cut(s)	432, 561
MhlI	GDGCHC	1 cut(s)	244
MluCI	AATT	5 cut(s)	138, 180, 216, 249, 315
MlyI	GAGTC	1 cut(s)	41
MmeI	TCCRAC	1 cut(s)	37
MnlI	CCTC	1 cut(s)	72
MseI	TTAA	5 cut(s)	27, 135, 171, 297, 308
MslI	CAYNNNNRTG	1 cut(s)	491
MspA1I	CMGCKG	1 cut(s)	567
MssI	GTTTAAAC	1 cut(s)	28
MvnI	CGCG	1 cut(s)	567
MwoI	GCNNNNNNNGC	5 cut(s)	114, 461, 488, 497, 556
NdeII	GATC	3 cut(s)	370, 432, 561
NheI	GCTAGC	1 cut(s)	329
NlaIII	CATG	4 cut(s)	69, 248, 445, 490
NlaIV	GGNNCC	1 cut(s)	434
NmuCI	GTSAC	1 cut(s)	268
PfeI	GAWTC	2 cut(s)	4, 92
PkrI	GCNGC	4 cut(s)	499, 549, 596, 599
PleI	GAGTC	1 cut(s)	41
PmeI	GTTTAAAC	1 cut(s)	28
PpsI	GAGTC	1 cut(s)	41
PsiI	TTATAA	1 cut(s)	254
Psp1406I	AACGTT	1 cut(s)	205
PspN4I	GGNNCC	1 cut(s)	434
PspPI	GGNCC	1 cut(s)	274
PsuI	RGATCY	2 cut(s)	432, 561
RseI	CAYNNNNRTG	1 cut(s)	491
SacII	CCGCGG	1 cut(s)	568
SaqAI	TTAA	5 cut(s)	27, 135, 171, 297, 308
SatI	GCNGC	4 cut(s)	498, 548, 595, 598
Sau3AI	GATC	3 cut(s)	370, 432, 561
Sau96I	GGNCC	1 cut(s)	274
SchI	GAGTC	1 cut(s)	41
SduI	GDGCHC	1 cut(s)	244
SetI	ASST	8 cut(s)	83, 110, 208, 230, 287, 351, 409, 577
Sfr303I	CCGCGG	1 cut(s)	568
SgrBI	CCGCGG	1 cut(s)	568
SinI	GGWCC	1 cut(s)	274
SmiMI	CAYNNNNRTG	1 cut(s)	491
SpeI	ACTAGT	2 cut(s)	221, 449
Sse9I	AATT	5 cut(s)	138, 180, 216, 249, 315
SsiI	CCGC	4 cut(s)	419, 548, 565, 567
SspMI	CTAG	3 cut(s)	222, 330, 450
TaaI	ACNGT	4 cut(s)	148, 236, 323, 556
TaiI	ACGT	1 cut(s)	208
TasI	AATT	5 cut(s)	138, 180, 216, 249, 315
TauI	GCSGC	1 cut(s)	550
TfiI	GAWTC	2 cut(s)	4, 92
Tru1I	TTAA	5 cut(s)	27, 135, 171, 297, 308
Tru9I	TTAA	5 cut(s)	27, 135, 171, 297, 308
TscAI	CASTG	3 cut(s)	196, 382, 561
TseFI	GTSAC	1 cut(s)	268
TseI	GCWGC	3 cut(s)	497, 594, 597
Tsp45I	GTSAC	1 cut(s)	268
TspDTI	ATGAA	7 cut(s)	17, 84, 327, 414, 430, 475, 548
TspGWI	ACGGA	1 cut(s)	287
TspRI	CASTG	3 cut(s)	196, 382, 561
VpaK11BI	GGWCC	1 cut(s)	274
XapI	RAATTY	2 cut(s)	138, 180
XspI	CTAG	3 cut(s)	222, 330, 450

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.