Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0015808

Physical Location & Seq

Genomic Coordinates Reverse (-)

5925698 .. 5927942

Gene Structure 2245 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1050400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 609 bp

ATGAATCCCGAATATGACTATTTGTTTAAGCTTTTGCTGATTGGAGATTCCGGTGTCGGCAAGTCATGTCTACTTCTGCGGTTTGCTGATGATTCGTACTTGGATAGCTACATCAGCACAATTGGAGTTGACTTTAAAATTCGCACTGTCGAGCAGGATGGGAAGACTATCAAACTTCAAATTTGGGATACTGCTGGCCAAGAACGTTTTAGGACAATCACTAGCAGCTACTACCGTGGGGCTCATGGTATCATTGTTGTTTATGATGTCACAGACCAAGAGAGCTTCAACAATGTTAAGCAATGGCTGAATGAAATCGACCGATATGCAAGTGAAAATGTCAACAAGCTTCTAGTTGGAAACAAGTGCGATCTCACAGCAAATAAAGTTGTGTCCTATGAGACAGCCAAGGCATTTGCAGATGAAATTGGAATCCCATTCATGGAAACAAGTGCCAAGAATTCCACCAATGTTGAGCAGGCCTTCATGGCTATGGCTGCTGAGATCAAGAACAGGATGGCAAGCCAACCGATGAACAACACCAGGCCTCCAACAGTGCAGATTAGGGGACAGCCTGTGAACCAAAAGTCTGGTTGCTGCTCATCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000139 GO:0000902 GO:0000904 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005783 GO:0005789 GO:0005794 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006888 GO:0007275 GO:0008150 GO:0009506 GO:0009555 GO:0009653 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0016192 GO:0022414 GO:0030054 GO:0030154 GO:0031090 GO:0031984 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0044706 GO:0046907 GO:0048193 GO:0048229 GO:0048468 GO:0048588 GO:0048589 GO:0048856 GO:0048868 GO:0048869 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051704 GO:0055044 GO:0060560 GO:0071840 GO:0071944 GO:0098588 GO:0098791 GO:0098827

KEGG Pathways

Metabolic & Signaling

ko05134 map05134

Pfam Domains

Protein Families

Ras

Protein Analysis

203

Amino Acids

22.65

Weight (kDa)

5.52

Isoelectric Point (pI)

31.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Arf	PF00025	9 - 129	1.1e-16	ADP-ribosylation factor family
Ras	PF00071	10 - 170	2.7e-69	Ras family
Roc	PF08477	10 - 124	3.4e-37	Ras of Complex, Roc, domain of DAPkinase
GTP_EFTU	PF00009	45 - 166	2.7e-06	Elongation factor Tu GTP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015808)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G02130
fragaria_vesca	FvH4_7g26840
malus_domestica	MD01G1176600.v1.1 MD04G1050400.v1.1 MD07G1247800.v1.1
prunus_persica	Prupe.2G271800_v2.0.a1 Prupe.2G271800_v2.0.a1
pyrus_communis	pycom01g18730
rosa_chinensis	RchiOBHm_Chr1g0373531
rosa_laevigata	RLG00000026818
rosa_roxburghii	Rroxscaffold_4G00284170
rosa_rugosa	Rorug01G0377900 Rorug01G0378000
rosa_samantha	Rh1CG364400
rosa_wichuraiana	Rw1G034800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	70
AciI	CCGC	1 cut(s)	79
AclI	AACGTT	1 cut(s)	205
AcoI	YGGCCR	1 cut(s)	196
AcsI	RAATTY	3 cut(s)	138, 180, 460
AfaI	GTAC	1 cut(s)	98
AfiI	CCNNNNNNNGG	1 cut(s)	442
AgsI	TTSAA	2 cut(s)	179, 289
AjnI	CCWGG	1 cut(s)	542
AluBI	AGCT	5 cut(s)	31, 108, 228, 285, 349
AluI	AGCT	5 cut(s)	31, 108, 228, 285, 349
Alw26I	GTCTC	1 cut(s)	395
AoxI	GGCC	3 cut(s)	196, 480, 545
ApeKI	GCWGC	3 cut(s)	225, 497, 597
ApoI	RAATTY	3 cut(s)	138, 180, 460
BalI	TGGCCA	1 cut(s)	198
BanII	GRGCYC	1 cut(s)	244
BbsI	GAAGAC	1 cut(s)	170
BbvI	GCAGC	3 cut(s)	237, 484, 584
BccI	CCATC	2 cut(s)	152, 511
BcgI	CGANNNNNNTGC	2 cut(s)	308, 342
BciT130I	CCWGG	1 cut(s)	544
BciVI	GTATCC	1 cut(s)	181
BcoDI	GTCTC	1 cut(s)	395
BfaI	CTAG	2 cut(s)	222, 353
BfuI	GTATCC	1 cut(s)	181
BglI	GCCNNNNNGGC	1 cut(s)	488
BisI	GCNGC	3 cut(s)	226, 498, 598
BlsI	GCNGC	3 cut(s)	227, 499, 599
Bme1390I	CCNGG	1 cut(s)	544
BmrFI	CCNGG	1 cut(s)	544
BpiI	GAAGAC	1 cut(s)	170
BsaJI	CCNNGG	2 cut(s)	235, 408
BsaWI	WCCGGW	1 cut(s)	50
BsaXI	ACNNNNNCTCC	2 cut(s)	532, 562
Bsc4I	CCNNNNNNNGG	1 cut(s)	442
Bse3DI	GCAATG	1 cut(s)	308
BseBI	CCWGG	1 cut(s)	544
BseDI	CCNNGG	2 cut(s)	235, 408
BseGI	GGATG	2 cut(s)	163, 522
BseLI	CCNNNNNNNGG	1 cut(s)	442
BseMI	GCAATG	1 cut(s)	308
BseMII	CTCAG	1 cut(s)	492
BseXI	GCAGC	3 cut(s)	237, 484, 584
BsgI	GTGCAG	1 cut(s)	578
Bsh1285I	CGRYCG	1 cut(s)	322
BshFI	GGCC	3 cut(s)	198, 482, 547
BsiEI	CGRYCG	1 cut(s)	322
BsiSI	CCGG	1 cut(s)	51
BslFI	GGGAC	1 cut(s)	582
BslI	CCNNNNNNNGG	1 cut(s)	442
BsmAI	GTCTC	1 cut(s)	395
BsmFI	GGGAC	1 cut(s)	582
BsnI	GGCC	3 cut(s)	198, 482, 547
Bsp1286I	GDGCHC	1 cut(s)	244
Bsp143I	GATC	2 cut(s)	370, 504
BspACI	CCGC	1 cut(s)	79
BspANI	GGCC	3 cut(s)	198, 482, 547
BspCNI	CTCAG	1 cut(s)	493
BsrDI	GCAATG	1 cut(s)	308
BssECI	CCNNGG	2 cut(s)	235, 408
BssMI	GATC	2 cut(s)	370, 504
BssT1I	CCWWGG	1 cut(s)	408
Bst2UI	CCWGG	1 cut(s)	544
Bst4CI	ACNGT	3 cut(s)	148, 236, 556
BstC8I	GCNNGC	3 cut(s)	196, 480, 523
BstDEI	CTNAG	2 cut(s)	501, 606
BstDSI	CCRYGG	1 cut(s)	235
BstF5I	GGATG	2 cut(s)	163, 522
BstKTI	GATC	2 cut(s)	373, 507
BstMAI	GTCTC	1 cut(s)	395
BstMBI	GATC	2 cut(s)	370, 504
BstMCI	CGRYCG	1 cut(s)	322
BstMWI	GCNNNNNNNGC	3 cut(s)	114, 488, 497
BstNI	CCWGG	1 cut(s)	544
BstSCI	CCNGG	1 cut(s)	542
BstV1I	GCAGC	3 cut(s)	237, 484, 584
BstV2I	GAAGAC	1 cut(s)	170
BstXI	CCANNNNNNTGG	1 cut(s)	590
BsuI	GTATCC	1 cut(s)	181
BsuRI	GGCC	3 cut(s)	198, 482, 547
BtgI	CCRYGG	1 cut(s)	235
BtsCI	GGATG	2 cut(s)	163, 522
BtsIMutI	CAGTG	2 cut(s)	144, 561
Cac8I	GCNNGC	3 cut(s)	196, 480, 523
Csp6I	GTAC	1 cut(s)	97
CspCI	CAANNNNNGTGG	2 cut(s)	454, 489
CviAII	CATG	4 cut(s)	66, 245, 442, 487
CviQI	GTAC	1 cut(s)	97
DdeI	CTNAG	2 cut(s)	501, 606
DpnI	GATC	2 cut(s)	372, 506
DpnII	GATC	2 cut(s)	370, 504
DraI	TTTAAA	1 cut(s)	136
EaeI	YGGCCR	1 cut(s)	196
Eco130I	CCWWGG	1 cut(s)	408
Eco147I	AGGCCT	2 cut(s)	482, 547
Eco24I	GRGCYC	1 cut(s)	244
EcoRI	GAATTC	1 cut(s)	460
EcoRII	CCWGG	1 cut(s)	542
EcoT14I	CCWWGG	1 cut(s)	408
EcoT38I	GRGCYC	1 cut(s)	244
ErhI	CCWWGG	1 cut(s)	408
FaeI	CATG	4 cut(s)	69, 248, 445, 490
FaiI	YATR	9 cut(s)	15, 67, 246, 264, 327, 399, 443, 488, 494
FaqI	GGGAC	1 cut(s)	582
FatI	CATG	4 cut(s)	65, 244, 441, 486
FblI	GTMKAC	1 cut(s)	70
Fnu4HI	GCNGC	3 cut(s)	226, 498, 598
FokI	GGATG	2 cut(s)	170, 529
FriOI	GRGCYC	1 cut(s)	244
Fsp4HI	GCNGC	3 cut(s)	226, 498, 598
FspBI	CTAG	2 cut(s)	222, 353
GluI	GCNGC	3 cut(s)	226, 498, 598
HaeIII	GGCC	3 cut(s)	198, 482, 547
HapII	CCGG	1 cut(s)	51
Hin1II	CATG	4 cut(s)	69, 248, 445, 490
HincII	GTYRAC	2 cut(s)	130, 343
HindII	GTYRAC	2 cut(s)	130, 343
HindIII	AAGCTT	2 cut(s)	29, 347
HinfI	GANTC	4 cut(s)	4, 47, 92, 432
HpaII	CCGG	1 cut(s)	51
Hpy166II	GTNNAC	4 cut(s)	71, 130, 343, 580
Hpy188III	TCNNGA	2 cut(s)	8, 508
Hpy8I	GTNNAC	4 cut(s)	71, 130, 343, 580
HpyAV	CCTTC	1 cut(s)	493
HpyCH4III	ACNGT	3 cut(s)	148, 236, 556
HpyCH4IV	ACGT	1 cut(s)	205
HpyCH4V	TGCA	3 cut(s)	329, 419, 559
HpyF10VI	GCNNNNNNNGC	3 cut(s)	114, 488, 497
HpyF3I	CTNAG	2 cut(s)	501, 606
HpySE526I	ACGT	1 cut(s)	205
Hsp92II	CATG	4 cut(s)	69, 248, 445, 490
Kzo9I	GATC	2 cut(s)	370, 504
LpnPI	CCDG	9 cut(s)	64, 140, 180, 464, 499, 529, 556, 576, 588
Lsp1109I	GCAGC	3 cut(s)	237, 484, 584
MaeI	CTAG	2 cut(s)	222, 353
MaeII	ACGT	1 cut(s)	205
MaeIII	GTNAC	1 cut(s)	268
MalI	GATC	2 cut(s)	372, 506
MboI	GATC	2 cut(s)	370, 504
MboII	GAAGA	1 cut(s)	175
MfeI	CAATTG	1 cut(s)	120
MhlI	GDGCHC	1 cut(s)	244
MlsI	TGGCCA	1 cut(s)	198
MluCI	AATT	5 cut(s)	120, 138, 180, 426, 460
MluNI	TGGCCA	1 cut(s)	198
MmeI	TCCRAC	2 cut(s)	337, 575
MnlI	CCTC	1 cut(s)	558
Mox20I	TGGCCA	1 cut(s)	198
MscI	TGGCCA	1 cut(s)	198
MseI	TTAA	3 cut(s)	27, 135, 297
MslI	CAYNNNNRTG	1 cut(s)	491
Msp20I	TGGCCA	1 cut(s)	198
MspI	CCGG	1 cut(s)	51
MspR9I	CCNGG	1 cut(s)	544
MunI	CAATTG	1 cut(s)	120
MvaI	CCWGG	1 cut(s)	544
MwoI	GCNNNNNNNGC	3 cut(s)	114, 488, 497
NdeII	GATC	2 cut(s)	370, 504
NlaIII	CATG	4 cut(s)	69, 248, 445, 490
NmuCI	GTSAC	1 cut(s)	268
PceI	AGGCCT	2 cut(s)	482, 547
PfeI	GAWTC	4 cut(s)	4, 47, 92, 432
PkrI	GCNGC	3 cut(s)	227, 499, 599
Psp1406I	AACGTT	1 cut(s)	205
Psp6I	CCWGG	1 cut(s)	542
PspGI	CCWGG	1 cut(s)	542
RsaI	GTAC	1 cut(s)	98
RsaNI	GTAC	1 cut(s)	97
RseI	CAYNNNNRTG	1 cut(s)	491
SaqAI	TTAA	3 cut(s)	27, 135, 297
SatI	GCNGC	3 cut(s)	226, 498, 598
Sau3AI	GATC	2 cut(s)	370, 504
ScrFI	CCNGG	1 cut(s)	544
SduI	GDGCHC	1 cut(s)	244
SetI	ASST	6 cut(s)	33, 110, 208, 230, 287, 351
SmiMI	CAYNNNNRTG	1 cut(s)	491
Sse9I	AATT	5 cut(s)	120, 138, 180, 426, 460
SseBI	AGGCCT	2 cut(s)	482, 547
SsiI	CCGC	1 cut(s)	79
SspMI	CTAG	2 cut(s)	222, 353
StuI	AGGCCT	2 cut(s)	482, 547
StyD4I	CCNGG	1 cut(s)	542
StyI	CCWWGG	1 cut(s)	408
TaaI	ACNGT	3 cut(s)	148, 236, 556
TaiI	ACGT	1 cut(s)	208
TaqI	TCGA	2 cut(s)	150, 318
TaqII	GACCGA	1 cut(s)	336
TasI	AATT	5 cut(s)	120, 138, 180, 426, 460
TfiI	GAWTC	4 cut(s)	4, 47, 92, 432
Tru1I	TTAA	3 cut(s)	27, 135, 297
Tru9I	TTAA	3 cut(s)	27, 135, 297
TscAI	CASTG	2 cut(s)	151, 561
TseFI	GTSAC	1 cut(s)	268
TseI	GCWGC	3 cut(s)	225, 497, 597
Tsp45I	GTSAC	1 cut(s)	268
TspDTI	ATGAA	6 cut(s)	17, 327, 430, 438, 475, 548
TspRI	CASTG	2 cut(s)	151, 561
XapI	RAATTY	3 cut(s)	138, 180, 460
XmiI	GTMKAC	1 cut(s)	70
XspI	CTAG	2 cut(s)	222, 353

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.