MD01G1197400.v1.1

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013803
Physical Location & Seq
Forward (+)
29557189 .. 29561731
4543 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1197400.v1.1.491

Sequence Viewer

Length: 1944 bp
ATGGCTGCCAAGGACGACCGCCTTCCGTACCACCCAACCTCCAAGCCCTCCAAACCCTCTCCATTCATTTGGAAAAAACCCAACGTCTACACCTTACCCGTCGTCCTCTTCCTCTGCATCTGCTCCTACTTTTTCGGCCTCTGGCAACACGGCGGCCCCACCGCGGTCACTAAAACCGCCTCCACGCCAACCTTCAAACTCCCATGCAAATCCCCCAACGAAATCCACACCACAACCACAACAACCACCGCGTCAGCCTTAGATTTCACCTCCCACCACAGCCCCAACTTCAGCACCGCCACGTCCTCCCACGCCACCACCACCAACGTGTTCCCGCGCTGCGATGCGAAATACAGCGAGTACACGCCATGCGAGGACGACAAGAGGTCGCTGAAGTTTGACCGTGACAGGCTCATATACAGAGAGAGGCACTGCCCCGAGAAGAGCGAGCTCCTTAAGTGCCGCATCCCAGCACCGCACGGCTACAAAAACGTGTTCCCGTGGCCGAGAAGCCGGGATCTGGCTTGGTACGCCAATGCGCCGCACAAGGAGTTGACGGTGGAGAAGGCCGTGCAGAATTGGATCATATACGAAGGAGATAGGTTTAGGTTCCCTGGGGGAGGCACCATGTTCCCTAACGGTGCGGACGCGTACATTGATGATATTGGCAAATTCATCAATCTCAAAGATGGCTCGATTCGGACCGCCATTGATACAGGCTGTGGGGTTGCGAGTTGGGGAGCGTACCTGTTGTCCCGCGACATCCTCGCCATGTCGTTCGCGCCGAGGGACACGCACGTGTCGCAGGTCCAGTTTGCTCTCGAGCGCGGAGTGCCCGCCCTCATCGGAGTCATTGCGTCGAAAAGACTTCCTTACCCTTCTCGAGCCTTTGACATGGCCCATTGCTCTCGCTGCCTCATTCCCTGGGGGCAACACGATGGGGTTTACTTAACGGAGGTGGACAGAGTTCTGAGACCGGGCGGGTACTGGATTCTATCGGGGCCGCCGATCCGTTGGGCAAAATATTGGAAGGGTTGGGAAAGAACCAAAGAGGATTTGAATGAAGAGCAGACTTCCATTGAGAATGTAGCCAAGAGCCTCTGCTGGAAAAAGTTGGCGGAGAAGGACGACATTGCCATTTGGCAAAAACCCACCAACCATTTGAGTTGCCAAAACAACCGCAAGTTCGGCAAGGCTCGCAATTTCTGTCCCGCCAATGATCCTGATACCGCCTGGTACACAAAGTTGGAGACTTGTTTAACCCCATTGCCAGAAGTTTCAGACAATGAAGAAGTTGCAGGAGGGAAGTTGGCAAACTGGCCACATAGGCTCAATGCAATCCCACCAAGGATTAGCAAGGGAACTGTGAAAGGTGTGACGGCTGACGTTTTTAAGCAAGATTCGGAGCTGTGGAAGAAGAGAGTTTCGTACTATAAATCGGTGAATAATCAGCTGGGAAACCCTGGAAGGTATAGAAATATTTTGGACATGAATGCACAGTTGGGTGGATTTGCTGCTGCCCTAGTTGATCTCCCTGTCTGGGTCATGAATGTGGTTCCGGTGGAGGTTAAGTCTAACACCCTTGGAGTCATCTATGAACGGGGATTGATCGGAACATATCAGAACTGGTGTGAAGCAATGTCTACATATCCAAGAACTTATGACTTCCTTCATGCTGATTCAGTGTTTAGCCTCTACAAAGATAGATGCGAAGCGGAAGACATTTTGCTGGAGATGGACAGAATTCTGAGGCCGGAGGGGAGCGTGGTCATGCGAGACGATGTTGATGTGCTGGTGAAGATCAAGAGCATCGTCGACGGAATGGAATGGGACAGCCAGATTGTAGACCACGAAGACGGTCCTCTCGAGAGGGAAAAACTGCTGTTCGCCGTGAAAAAGTATTGGACAGCTCCTGCTGCAGCTGAAGATAAACCTTCCTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

648

Amino Acids

73.24

Weight (kDa)

8.31

Isoelectric Point (pI)

31.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 120 - 632 3.1e-228 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 796
AccB1I GGYRCC 1 cut(s) 623
AccI GTMKAC 4 cut(s) 87, 1643, 1815, 1845
AccII CGCG 7 cut(s) 164, 251, 337, 650, 759, 782, 828
AclWI GGATC 4 cut(s) 525, 590, 1003, 1214
AcoI YGGCCR 2 cut(s) 503, 1319
AcsI RAATTY 2 cut(s) 671, 1743
AcuI CTGAAG 3 cut(s) 274, 413, 1944
AcvI CACGTG 1 cut(s) 799
AfaI GTAC 8 cut(s) 29, 362, 530, 653, 746, 986, 1238, 1430
AfiI CCNNNNNNNGG 4 cut(s) 163, 520, 620, 1540
AflII CTTAAG 1 cut(s) 455
AflIII ACRYGT 4 cut(s) 327, 492, 648, 798
AgsI TTSAA 2 cut(s) 196, 1060
AhdI GACNNNNNGTC 1 cut(s) 385
AjiI CACGTC 1 cut(s) 303
AjnI CCWGG 4 cut(s) 613, 923, 1232, 1462
AjuI GAANNNNNNNTTGG 2 cut(s) 1484, 1516
AleI CACNNNNGTG 2 cut(s) 326, 797
AluBI AGCT 5 cut(s) 451, 1408, 1453, 1910, 1922
AluI AGCT 5 cut(s) 451, 1408, 1453, 1910, 1922
Alw21I GWGCWC 1 cut(s) 453
Alw26I GTCTC 3 cut(s) 967, 1244, 1770
AlwI GGATC 4 cut(s) 525, 590, 1003, 1214
AlwNI CAGNNNCTG 1 cut(s) 1913
Ama87I CYCGRG 4 cut(s) 437, 821, 882, 1865
AoxI GGCC 8 cut(s) 136, 154, 503, 567, 897, 1001, 1319, 1751
ApeKI GCWGC 7 cut(s) 5, 339, 912, 1514, 1517, 1916, 1919
ApoI RAATTY 2 cut(s) 671, 1743
AspLEI GCGC 4 cut(s) 339, 541, 784, 828
AspS9I GGNCC 6 cut(s) 155, 702, 808, 898, 1001, 1859
AsuC2I CCSGG 2 cut(s) 515, 978
AsuHPI GGTGA 3 cut(s) 259, 1453, 1807
AvaI CYCGRG 4 cut(s) 437, 821, 882, 1865
AvaII GGWCC 3 cut(s) 702, 808, 1859
BaeGI GKGCMC 1 cut(s) 837
BalI TGGCCA 1 cut(s) 1321
BanI GGYRCC 1 cut(s) 623
BanII GRGCYC 1 cut(s) 453
BbrPI CACGTG 1 cut(s) 799
BbsI GAAGAC 2 cut(s) 1725, 1860
Bbv12I GWGCWC 1 cut(s) 453
BbvI GCAGC 6 cut(s) 326, 899, 1501, 1504, 1903, 1931
BccI CCATC 3 cut(s) 683, 932, 1729
BceAI ACGGC 5 cut(s) 166, 496, 554, 1395, 1874
BciT130I CCWGG 4 cut(s) 615, 925, 1234, 1464
BcnI CCSGG 2 cut(s) 515, 978
BcoDI GTCTC 3 cut(s) 967, 1244, 1770
BfaI CTAG 2 cut(s) 1523, 1942
BfmI CTRYAG 1 cut(s) 1917
BfrI CTTAAG 1 cut(s) 455
BfuAI ACCTGC 1 cut(s) 796
BglI GCCNNNNNGGC 1 cut(s) 1327
Bme1390I CCNGG 6 cut(s) 515, 615, 925, 978, 1234, 1464
Bme18I GGWCC 3 cut(s) 702, 808, 1859
BmeRI GACNNNNNGTC 1 cut(s) 385
BmeT110I CYCGRG 4 cut(s) 437, 821, 882, 1865
BmgBI CACGTC 1 cut(s) 303
BmgT120I GGNCC 6 cut(s) 155, 702, 808, 898, 1001, 1859
BmiI GGNNCC 5 cut(s) 157, 611, 625, 1002, 1557
BmrFI CCNGG 6 cut(s) 515, 615, 925, 978, 1234, 1464
BmsI GCATC 5 cut(s) 126, 334, 474, 1697, 1818
BpiI GAAGAC 2 cut(s) 1725, 1860
BpmI CTGGAG 1 cut(s) 1751
BpuMI CCSGG 2 cut(s) 515, 978
BsaAI YACGTR 1 cut(s) 799
BsaI GGTCTC 1 cut(s) 967
BsaWI WCCGGW 1 cut(s) 1558
BsaXI ACNNNNNCTCC 4 cut(s) 23, 53, 1556, 1586
Bsc4I CCNNNNNNNGG 4 cut(s) 163, 520, 620, 1540
Bse1I ACTGG 4 cut(s) 811, 992, 1322, 1631
Bse3DI GCAATG 5 cut(s) 852, 901, 1131, 1265, 1644
BseBI CCWGG 4 cut(s) 615, 925, 1234, 1464
BseGI GGATG 2 cut(s) 465, 762
BseLI CCNNNNNNNGG 4 cut(s) 163, 520, 620, 1540
BseMI GCAATG 5 cut(s) 852, 901, 1131, 1265, 1644
BseMII CTCAG 2 cut(s) 962, 1739
BseNI ACTGG 4 cut(s) 811, 992, 1322, 1631
BseSI GKGCMC 1 cut(s) 837
BseXI GCAGC 6 cut(s) 326, 899, 1501, 1504, 1903, 1931
BseYI CCCAGC 2 cut(s) 469, 1453
BsgI GTGCAG 1 cut(s) 593
Bsh1236I CGCG 7 cut(s) 164, 251, 337, 650, 759, 782, 828
Bsh1285I CGRYCG 1 cut(s) 19
BshFI GGCC 8 cut(s) 138, 156, 505, 569, 899, 1003, 1321, 1753
BshNI GGYRCC 1 cut(s) 623
BsiEI CGRYCG 1 cut(s) 19
BsiHKAI GWGCWC 1 cut(s) 453
BsiHKCI CYCGRG 4 cut(s) 437, 821, 882, 1865
BsiSI CCGG 4 cut(s) 514, 977, 1559, 1754
BslFI GGGAC 4 cut(s) 739, 803, 1194, 1844
BslI CCNNNNNNNGG 4 cut(s) 163, 520, 620, 1540
BsmAI GTCTC 3 cut(s) 967, 1244, 1770
BsmBI CGTCTC 1 cut(s) 1770
BsmFI GGGAC 4 cut(s) 739, 803, 1194, 1844
BsmI GAATGC 1 cut(s) 1498
BsnI GGCC 8 cut(s) 138, 156, 505, 569, 899, 1003, 1321, 1753
Bso31I GGTCTC 1 cut(s) 967
BsoBI CYCGRG 4 cut(s) 437, 821, 882, 1865
Bsp1286I GDGCHC 2 cut(s) 453, 837
Bsp143I GATC 7 cut(s) 517, 582, 1008, 1219, 1528, 1608, 1800
BspANI GGCC 8 cut(s) 138, 156, 505, 569, 899, 1003, 1321, 1753
BspCNI CTCAG 2 cut(s) 963, 1740
BspFNI CGCG 7 cut(s) 164, 251, 337, 650, 759, 782, 828
BspHI TCATGA 1 cut(s) 1545
BspLI GGNNCC 5 cut(s) 157, 611, 625, 1002, 1557
BspMAI CTGCAG 1 cut(s) 1921
BspMI ACCTGC 1 cut(s) 796
BspPI GGATC 4 cut(s) 525, 590, 1003, 1214
BspQI GCTCTTC 2 cut(s) 437, 1059
BspT107I GGYRCC 1 cut(s) 623
BspTI CTTAAG 1 cut(s) 455
BspTNI GGTCTC 1 cut(s) 967
BsrDI GCAATG 5 cut(s) 852, 901, 1131, 1265, 1644
BsrI ACTGG 4 cut(s) 811, 992, 1322, 1631
BssMI GATC 7 cut(s) 517, 582, 1008, 1219, 1528, 1608, 1800
BssT1I CCWWGG 3 cut(s) 9, 1346, 1582
Bst2UI CCWGG 4 cut(s) 615, 925, 1234, 1464
Bst4CI ACNGT 6 cut(s) 404, 559, 641, 1366, 1500, 1859
Bst6I CTCTTC 4 cut(s) 113, 437, 1059, 1412
BstAFI CTTAAG 1 cut(s) 455
BstBAI YACGTR 1 cut(s) 799
BstC8I GCNNGC 3 cut(s) 449, 837, 1198
BstDEI CTNAG 3 cut(s) 259, 971, 1748
BstDSI CCRYGG 2 cut(s) 162, 500
BstENI CCTNNNNNAGG 1 cut(s) 618
BstF5I GGATG 2 cut(s) 465, 762
BstFNI CGCG 7 cut(s) 164, 251, 337, 650, 759, 782, 828
BstHHI GCGC 4 cut(s) 339, 541, 784, 828
BstKTI GATC 7 cut(s) 520, 585, 1011, 1222, 1531, 1611, 1803
BstMAI GTCTC 3 cut(s) 967, 1244, 1770
BstMBI GATC 7 cut(s) 517, 582, 1008, 1219, 1528, 1608, 1800
BstMCI CGRYCG 1 cut(s) 19
BstMWI GCNNNNNNNGC 8 cut(s) 530, 802, 832, 912, 1188, 1197, 1327, 1916
BstNI CCWGG 4 cut(s) 615, 925, 1234, 1464
BstSCI CCNGG 6 cut(s) 513, 613, 923, 976, 1232, 1462
BstSFI CTRYAG 1 cut(s) 1917
BstSLI GKGCMC 1 cut(s) 837
BstUI CGCG 7 cut(s) 164, 251, 337, 650, 759, 782, 828
BstV1I GCAGC 6 cut(s) 326, 899, 1501, 1504, 1903, 1931
BstV2I GAAGAC 2 cut(s) 1725, 1860
BstX2I RGATCY 1 cut(s) 517
BstXI CCANNNNNNTGG 1 cut(s) 69
BstYI RGATCY 1 cut(s) 517
BsuRI GGCC 8 cut(s) 138, 156, 505, 569, 899, 1003, 1321, 1753
BtgI CCRYGG 2 cut(s) 162, 500
BtgZI GCGATG 1 cut(s) 357
BtrI CACGTC 1 cut(s) 303
BtsCI GGATG 2 cut(s) 465, 762
BtsI GCAGTG 1 cut(s) 430
BtsIMutI CAGTG 2 cut(s) 430, 1689
BveI ACCTGC 1 cut(s) 796
Cac8I GCNNGC 3 cut(s) 449, 837, 1198
CaiI CAGNNNCTG 1 cut(s) 1913
CciI TCATGA 1 cut(s) 1545
CfoI GCGC 4 cut(s) 339, 541, 784, 828
Cfr13I GGNCC 6 cut(s) 155, 702, 808, 898, 1001, 1859
Cfr42I CCGCGG 1 cut(s) 165
CpoI CGGWCCG 1 cut(s) 702
CseI GACGC 3 cut(s) 240, 656, 846
Csp6I GTAC 8 cut(s) 28, 361, 529, 652, 745, 985, 1237, 1429
CspI CGGWCCG 1 cut(s) 702
CviAII CATG 9 cut(s) 204, 369, 628, 772, 895, 1489, 1546, 1673, 1771
CviQI GTAC 8 cut(s) 28, 361, 529, 652, 745, 985, 1237, 1429
DdeI CTNAG 3 cut(s) 259, 971, 1748
DpnI GATC 7 cut(s) 519, 584, 1010, 1221, 1530, 1610, 1802
DpnII GATC 7 cut(s) 517, 582, 1008, 1219, 1528, 1608, 1800
DriI GACNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 2 cut(s) 503, 1319
Eam1104I CTCTTC 4 cut(s) 113, 437, 1059, 1412
Eam1105I GACNNNNNGTC 1 cut(s) 385
EarI CTCTTC 4 cut(s) 113, 437, 1059, 1412
EciI GGCGGA 1 cut(s) 1133
Ecl136II GAGCTC 1 cut(s) 451
Eco130I CCWWGG 3 cut(s) 9, 1346, 1582
Eco24I GRGCYC 1 cut(s) 453
Eco31I GGTCTC 1 cut(s) 967
Eco47I GGWCC 3 cut(s) 702, 808, 1859
Eco53kI GAGCTC 1 cut(s) 451
Eco57I CTGAAG 3 cut(s) 274, 413, 1944
Eco72I CACGTG 1 cut(s) 799
Eco88I CYCGRG 4 cut(s) 437, 821, 882, 1865
EcoICRI GAGCTC 1 cut(s) 451
EcoNI CCTNNNNNAGG 1 cut(s) 618
EcoRI GAATTC 1 cut(s) 1743
EcoRII CCWGG 4 cut(s) 613, 923, 1232, 1462
EcoT14I CCWWGG 3 cut(s) 9, 1346, 1582
EcoT38I GRGCYC 1 cut(s) 453
ErhI CCWWGG 3 cut(s) 9, 1346, 1582
Esp3I CGTCTC 1 cut(s) 1770
FaeI CATG 9 cut(s) 207, 372, 631, 775, 898, 1492, 1549, 1676, 1774
FalI AAGNNNNNCTT 2 cut(s) 856, 888
FaqI GGGAC 4 cut(s) 739, 803, 1194, 1844
FatI CATG 9 cut(s) 203, 368, 627, 771, 894, 1488, 1545, 1672, 1770
FauI CCCGC 5 cut(s) 342, 764, 844, 974, 1219
FblI GTMKAC 4 cut(s) 87, 1643, 1815, 1845
FokI GGATG 2 cut(s) 452, 749
FriOI GRGCYC 1 cut(s) 453
FspBI CTAG 2 cut(s) 1523, 1942
GlaI GCGC 4 cut(s) 338, 540, 783, 827
GsaI CCCAGC 2 cut(s) 473, 1457
GsuI CTGGAG 1 cut(s) 1751
HaeIII GGCC 8 cut(s) 138, 156, 505, 569, 899, 1003, 1321, 1753
HapII CCGG 4 cut(s) 514, 977, 1559, 1754
HgaI GACGC 3 cut(s) 240, 656, 846
HhaI GCGC 4 cut(s) 339, 541, 784, 828
Hin1II CATG 9 cut(s) 207, 372, 631, 775, 898, 1492, 1549, 1676, 1774
Hin6I GCGC 4 cut(s) 337, 539, 782, 826
HinP1I GCGC 4 cut(s) 337, 539, 782, 826
HincII GTYRAC 2 cut(s) 555, 1816
HindII GTYRAC 2 cut(s) 555, 1816
HinfI GANTC 6 cut(s) 697, 849, 991, 1400, 1587, 1679
HpaII CCGG 4 cut(s) 514, 977, 1559, 1754
HphI GGTGA 3 cut(s) 259, 1453, 1807
Hpy166II GTNNAC 9 cut(s) 88, 363, 555, 946, 961, 1239, 1644, 1816, 1846
Hpy188I TCNGA 8 cut(s) 702, 848, 972, 1282, 1405, 1613, 1623, 1749
Hpy188III TCNNGA 7 cut(s) 821, 882, 1223, 1546, 1804, 1865, 1867
Hpy8I GTNNAC 9 cut(s) 88, 363, 555, 946, 961, 1239, 1644, 1816, 1846
Hpy99I CGWCG 4 cut(s) 104, 862, 1817, 1820
HpyCH4III ACNGT 6 cut(s) 404, 559, 641, 1366, 1500, 1859
HpyCH4IV ACGT 6 cut(s) 84, 302, 327, 492, 798, 1386
HpyCH4V TGCA 7 cut(s) 117, 207, 574, 1298, 1337, 1496, 1919
HpyF10VI GCNNNNNNNGC 8 cut(s) 530, 802, 832, 912, 1188, 1197, 1327, 1916
HpyF3I CTNAG 3 cut(s) 259, 971, 1748
HpySE526I ACGT 6 cut(s) 84, 302, 327, 492, 798, 1386
Hsp92II CATG 9 cut(s) 207, 372, 631, 775, 898, 1492, 1549, 1676, 1774
HspAI GCGC 4 cut(s) 337, 539, 782, 826
KspI CCGCGG 1 cut(s) 165
Kzo9I GATC 7 cut(s) 517, 582, 1008, 1219, 1528, 1608, 1800
LguI GCTCTTC 2 cut(s) 437, 1059
LmnI GCTCC 6 cut(s) 128, 456, 740, 1405, 1761, 1915
Lsp1109I GCAGC 6 cut(s) 326, 899, 1501, 1504, 1903, 1931
LweI GCATC 5 cut(s) 126, 334, 474, 1697, 1818
MaeI CTAG 2 cut(s) 1523, 1942
MaeII ACGT 6 cut(s) 84, 302, 327, 492, 798, 1386
MaeIII GTNAC 3 cut(s) 166, 404, 1375
MalI GATC 7 cut(s) 519, 584, 1010, 1221, 1530, 1610, 1802
MboI GATC 7 cut(s) 517, 582, 1008, 1219, 1528, 1608, 1800
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 2 cut(s) 453, 837
MlsI TGGCCA 1 cut(s) 1321
MluCI AATT 4 cut(s) 577, 671, 1201, 1743
MluI ACGCGT 1 cut(s) 648
MluNI TGGCCA 1 cut(s) 1321
MlyI GAGTC 2 cut(s) 858, 1596
MmeI TCCRAC 1 cut(s) 1227
Mox20I TGGCCA 1 cut(s) 1321
MscI TGGCCA 1 cut(s) 1321
MseI TTAA 5 cut(s) 456, 950, 1259, 1392, 1569
MslI CAYNNNNRTG 3 cut(s) 326, 797, 1550
Msp20I TGGCCA 1 cut(s) 1321
MspA1I CMGCKG 3 cut(s) 164, 1453, 1922
MspCI CTTAAG 1 cut(s) 455
MspI CCGG 4 cut(s) 514, 977, 1559, 1754
MspR9I CCNGG 6 cut(s) 515, 615, 925, 978, 1234, 1464
Mva1269I GAATGC 1 cut(s) 1498
MvaI CCWGG 4 cut(s) 615, 925, 1234, 1464
MvnI CGCG 7 cut(s) 164, 251, 337, 650, 759, 782, 828
MwoI GCNNNNNNNGC 8 cut(s) 530, 802, 832, 912, 1188, 1197, 1327, 1916
NciI CCSGG 2 cut(s) 515, 978
NdeII GATC 7 cut(s) 517, 582, 1008, 1219, 1528, 1608, 1800
NlaIII CATG 9 cut(s) 207, 372, 631, 775, 898, 1492, 1549, 1676, 1774
NlaIV GGNNCC 5 cut(s) 157, 611, 625, 1002, 1557
NmeAIII GCCGAG 2 cut(s) 531, 810
NmuCI GTSAC 3 cut(s) 166, 404, 1375
OliI CACNNNNGTG 2 cut(s) 326, 797
PaeR7I CTCGAG 3 cut(s) 821, 882, 1865
PagI TCATGA 1 cut(s) 1545
PasI CCCWGGG 2 cut(s) 614, 924
PciSI GCTCTTC 2 cut(s) 437, 1059
PcsI WCGNNNNNNNCGW 3 cut(s) 782, 1004, 1863
PctI GAATGC 1 cut(s) 1498
PfeI GAWTC 4 cut(s) 697, 991, 1400, 1679
PleI GAGTC 2 cut(s) 857, 1595
PmaCI CACGTG 1 cut(s) 799
PmlI CACGTG 1 cut(s) 799
PpsI GAGTC 2 cut(s) 857, 1595
Ppu21I YACGTR 1 cut(s) 799
Psp124BI GAGCTC 1 cut(s) 453
Psp6I CCWGG 4 cut(s) 613, 923, 1232, 1462
PspCI CACGTG 1 cut(s) 799
PspFI CCCAGC 2 cut(s) 469, 1453
PspGI CCWGG 4 cut(s) 613, 923, 1232, 1462
PspN4I GGNNCC 5 cut(s) 157, 611, 625, 1002, 1557
PspPI GGNCC 6 cut(s) 155, 702, 808, 898, 1001, 1859
PstI CTGCAG 1 cut(s) 1921
PstNI CAGNNNCTG 1 cut(s) 1913
PsuI RGATCY 1 cut(s) 517
PvuII CAGCTG 2 cut(s) 1453, 1922
RsaI GTAC 8 cut(s) 29, 362, 530, 653, 746, 986, 1238, 1430
RsaNI GTAC 8 cut(s) 28, 361, 529, 652, 745, 985, 1237, 1429
RseI CAYNNNNRTG 3 cut(s) 326, 797, 1550
Rsr2I CGGWCCG 1 cut(s) 702
RsrII CGGWCCG 1 cut(s) 702
SacI GAGCTC 1 cut(s) 453
SacII CCGCGG 1 cut(s) 165
SalI GTCGAC 1 cut(s) 1814
SapI GCTCTTC 2 cut(s) 437, 1059
SaqAI TTAA 5 cut(s) 456, 950, 1259, 1392, 1569
Sau3AI GATC 7 cut(s) 517, 582, 1008, 1219, 1528, 1608, 1800
Sau96I GGNCC 6 cut(s) 155, 702, 808, 898, 1001, 1859
SchI GAGTC 2 cut(s) 858, 1596
ScrFI CCNGG 6 cut(s) 515, 615, 925, 978, 1234, 1464
SduI GDGCHC 2 cut(s) 453, 837
SfaNI GCATC 5 cut(s) 126, 334, 474, 1697, 1818
SfcI CTRYAG 1 cut(s) 1917
Sfr274I CTCGAG 3 cut(s) 821, 882, 1865
Sfr303I CCGCGG 1 cut(s) 165
SgrBI CCGCGG 1 cut(s) 165
SgrDI CGTCGACG 1 cut(s) 1814
SinI GGWCC 3 cut(s) 702, 808, 1859
SlaI CTCGAG 3 cut(s) 821, 882, 1865
SmiMI CAYNNNNRTG 3 cut(s) 326, 797, 1550
SmlI CTYRAG 4 cut(s) 455, 821, 882, 1865
SmoI CTYRAG 4 cut(s) 455, 821, 882, 1865
Sse9I AATT 4 cut(s) 577, 671, 1201, 1743
SspI AATATT 2 cut(s) 1025, 1480
SspMI CTAG 2 cut(s) 1523, 1942
SstI GAGCTC 1 cut(s) 453
StyD4I CCNGG 6 cut(s) 513, 613, 923, 976, 1232, 1462
StyI CCWWGG 3 cut(s) 9, 1346, 1582
TaaI ACNGT 6 cut(s) 404, 559, 641, 1366, 1500, 1859
TaiI ACGT 6 cut(s) 87, 305, 330, 495, 801, 1389
TaqI TCGA 6 cut(s) 695, 822, 860, 883, 1815, 1866
TasI AATT 4 cut(s) 577, 671, 1201, 1743
TatI WGTACW 1 cut(s) 360
TauI GCSGC 4 cut(s) 156, 465, 544, 1006
TfiI GAWTC 4 cut(s) 697, 991, 1400, 1679
Tru1I TTAA 5 cut(s) 456, 950, 1259, 1392, 1569
Tru9I TTAA 5 cut(s) 456, 950, 1259, 1392, 1569
TscAI CASTG 2 cut(s) 437, 1689
TseFI GTSAC 3 cut(s) 166, 404, 1375
TseI GCWGC 7 cut(s) 5, 339, 912, 1514, 1517, 1916, 1919
Tsp45I GTSAC 3 cut(s) 166, 404, 1375
TspDTI ATGAA 8 cut(s) 55, 664, 1077, 1302, 1505, 1562, 1611, 1661
TspGWI ACGGA 4 cut(s) 15, 968, 1001, 1833
TspRI CASTG 2 cut(s) 437, 1689
Vha464I CTTAAG 1 cut(s) 455
VpaK11BI GGWCC 3 cut(s) 702, 808, 1859
XagI CCTNNNNNAGG 1 cut(s) 618
XapI RAATTY 2 cut(s) 671, 1743
XhoI CTCGAG 3 cut(s) 821, 882, 1865
XmiI GTMKAC 4 cut(s) 87, 1643, 1815, 1845
XspI CTAG 2 cut(s) 1523, 1942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.