Rroxscaffold_4G00281710

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013803
Physical Location & Seq
Reverse (-)
3896590 .. 3900339
3750 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281710.1

Sequence Viewer

Length: 1935 bp
ATGGCTTCCTCCGAGGACCACCATCCCTACCGCCCCACATCGAAACCCTCCAAGCCTTTTTCCCTTACCAACTTTTCCACATGGAAAATAAAACCCAACAACGCCTACACCTTGCCACTCATCCTCTTCCTGTGCATCTTCTCTTACTACTTCGGCCTCTGGCAACACAGCAGCACCGCCCCCGCCACCACCACCACCACCTCCGCCCTCAACCAACTTCCATGTAACTCCCCCGATACAACCCTAAACAAAAACCCTCAAAAAGCCCTAGATTTCACCACCCACCACAACCCTAACTTCGCCATCAACATTTCCAAACACAACACTCCCAAAACCTACCCTCCCTGCGACGTCAAGTACAGTGAGTACACCCCATGCGAGGACGCGAAGCGATCCTTGAAATTCGAGAGGGAAAGACTGATATACAGGGAGAGGCACTGTCCAGCTCAGGATGAGCTGCTCAAGTGCCGTGTTCCGGCACCTCACGGCTACCGGAACACGTTTACTTGGCCCAAAAGTAGAGATCTGGCGTGGTACGCCAATGTTCCGCACAAGGAGTTGACAGTGGAGAAGGCGGTGCAGAATTGGATTTTATACCAAGGCGACCGTTTTAAGTTCCCCGGAGGTGGAACCATGTTTCCGAATGGCGCCGATGCCTACATTGATGATATTGGGAAGTTGATCAATCTCAAGGACGGGTTTATTCGGACCGCCATTGATACAGGCTGTGGGGTTGCTAGTTGGGGAGCTTATCTATTGTCCCGGGATATTCTACCAATGTCTTTCGCGCCAAGAGACACACATGAAGCCCAAGTCCAATTCGCTCTCGAGCGAGGAGTACCCGCCCTCATCGGCGTCATTGCGTCGAAAAGACTTCCTTACCCTTCTAGAGCCTTTGACATGGCTCACTGCTCTCGTTGCCTCATTCCTTGGGGCCAATACGATGGAGTTTACTTGACGGAGGTTGATAGAGTTCTGAGGCCTGGTGGGTACTGGATTCTATCCGGCCCACCAATCCGCTGGAAGAAATACTGGAAGGGTTGGGAAAGAACACAGGAAGACTTGAACGAAGAACAAACTGGCATAGAGGATGTAGCCACTAGCCTTTGCTGGAAAAAGTTGGTGGAGAAGGACGACGTTGCAATTTGGCAAAAACCAACTAATCACTTAAACTGCAAAATGAACCGAAAGCTTGCCAAGAATCGCAACTTCTGCCCTGCTCAGGATCCTGATCAAGCTTGGTACACAGAGTTGGAGACTTGTTTGACTCCTTTACCGGAAGTTTCTAGCGAAGATGATGTTGCTGGTGGGAAGTTGGCGAAGTGGCCGGAGAGACTAACCTCAGTGCCGCCGAGAATTAGTAGGGGAACTGTGAAGGGTGTCACAGCTGAAGATTTTCAACAAGATTCGGAGCTATGGCGAAAAAGGGTAACACATTATAAAACAGTGAATAATCAACTTGCGCAGCCCGGAAGGTATAGAAACCTTCTGGATATGAATGCTTACTTGGGTGGATTTGCTGCTGCCCTAGTTGATCTGCCTGTTTGGGTCATGAATATGGTTCCCGTGGACGTGAAGGTTAACACACTCGGAGTCATCTATGAACGTGGATTGATCGGATCATATCAAAACTGGTGTGAAGCAATGTCTACATATCCAAGAACTTATGACCTCATTCATGCTGATACAGTGTTTAGTCTCTACAAGGACAGGTGCGAAGTAGAAGACATTTTACTAGAGATGGATAGAATTCTAAGGCCAGAGGGAAGCGTGATCATCCGAGACGACGTAGATTTGTTGGTGAAGATCAAGAGCATTATCGACCACATGGATTGGGATAGTCAAATTATTGATCACGAAAACGGACCTCATGAGAGAGAAAAGCTACTATTCGCGGTGAAAAAGTACTGGACTGCTCCCGCTCCGGCAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

644

Amino Acids

73.75

Weight (kDa)

7.23

Isoelectric Point (pI)

34.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 122 - 634 1.2e-227 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1440
AatII GACGTC 1 cut(s) 354
Acc16I TGCGCA 1 cut(s) 1464
AccB1I GGYRCC 2 cut(s) 478, 647
AccBSI CCGCTC 1 cut(s) 1922
AccI GTMKAC 1 cut(s) 1649
AccII CGCG 3 cut(s) 386, 788, 1895
AclWI GGATC 4 cut(s) 387, 1220, 1233, 1627
AcoI YGGCCR 1 cut(s) 1325
AcsI RAATTY 2 cut(s) 401, 1749
AcuI CTGAAG 1 cut(s) 1410
AcyI GRCGYC 3 cut(s) 351, 648, 855
AfaI GTAC 7 cut(s) 359, 368, 536, 840, 992, 1244, 1907
AfiI CCNNNNNNNGG 4 cut(s) 379, 475, 626, 1222
AflIII ACRYGT 1 cut(s) 498
AgsI TTSAA 3 cut(s) 400, 1066, 1400
AjiI CACGTC 1 cut(s) 1573
AjnI CCWGG 1 cut(s) 982
AjuI GAANNNNNNNTTGG 2 cut(s) 1490, 1522
AloI GAACNNNNNNTCC 2 cut(s) 622, 654
AluBI AGCT 8 cut(s) 446, 457, 749, 1192, 1238, 1388, 1414, 1885
AluI AGCT 8 cut(s) 446, 457, 749, 1192, 1238, 1388, 1414, 1885
Alw26I GTCTC 5 cut(s) 789, 1250, 1327, 1703, 1776
AlwI GGATC 4 cut(s) 387, 1220, 1233, 1627
Ama87I CYCGRG 2 cut(s) 762, 827
AoxI GGCC 7 cut(s) 154, 509, 934, 980, 1006, 1325, 1757
ApeKI GCWGC 5 cut(s) 171, 457, 1465, 1520, 1523
ApoI RAATTY 2 cut(s) 401, 1749
Asp700I GAANNNNTTC 1 cut(s) 1395
AspLEI GCGC 3 cut(s) 650, 790, 1465
AspS9I GGNCC 6 cut(s) 16, 510, 708, 934, 1007, 1865
AsuC2I CCSGG 4 cut(s) 621, 763, 764, 1470
AsuHPI GGTGA 3 cut(s) 268, 1813, 1909
AvaI CYCGRG 2 cut(s) 762, 827
AvaII GGWCC 3 cut(s) 16, 708, 1865
BamHI GGATCC 1 cut(s) 1225
BanI GGYRCC 2 cut(s) 478, 647
BarI GAAGNNNNNNTAC 2 cut(s) 1716, 1748
BbsI GAAGAC 2 cut(s) 1065, 1731
BbvI GCAGC 5 cut(s) 183, 444, 1477, 1507, 1510
BccI CCATC 4 cut(s) 30, 311, 938, 1735
BceAI ACGGC 2 cut(s) 453, 502
BciT130I CCWGG 1 cut(s) 984
BclI TGATCA 4 cut(s) 681, 1231, 1773, 1852
BcnI CCSGG 4 cut(s) 621, 763, 764, 1470
BcoDI GTCTC 5 cut(s) 789, 1250, 1327, 1703, 1776
BfaI CTAG 7 cut(s) 269, 738, 888, 1101, 1287, 1529, 1736
BfoI RGCGCY 1 cut(s) 651
BglII AGATCT 1 cut(s) 523
BisI GCNGC 6 cut(s) 172, 458, 1349, 1466, 1521, 1524
BlsI GCNGC 6 cut(s) 173, 459, 1350, 1467, 1522, 1525
BmcAI AGTACT 1 cut(s) 1907
Bme1390I CCNGG 5 cut(s) 621, 763, 764, 984, 1470
Bme18I GGWCC 3 cut(s) 16, 708, 1865
BmeT110I CYCGRG 2 cut(s) 762, 827
BmgBI CACGTC 1 cut(s) 1573
BmgT120I GGNCC 6 cut(s) 16, 510, 708, 934, 1007, 1865
BmiI GGNNCC 6 cut(s) 480, 631, 649, 935, 1227, 1563
BmrFI CCNGG 5 cut(s) 621, 763, 764, 984, 1470
BmsI GCATC 2 cut(s) 144, 643
BpiI GAAGAC 2 cut(s) 1065, 1731
Bpu10I CCTNAGC 2 cut(s) 447, 1221
BpuEI CTTGAG 2 cut(s) 446, 674
BpuMI CCSGG 4 cut(s) 621, 763, 764, 1470
BsaBI GATNNNNATC 1 cut(s) 1230
BsaHI GRCGYC 3 cut(s) 351, 648, 855
BsaJI CCNNGG 6 cut(s) 12, 598, 619, 762, 929, 1566
BsaWI WCCGGW 2 cut(s) 492, 1276
BsaXI ACNNNNNCTCC 2 cut(s) 325, 355
Bsc4I CCNNNNNNNGG 4 cut(s) 379, 475, 626, 1222
Bse1I ACTGG 5 cut(s) 998, 1037, 1084, 1637, 1913
Bse3DI GCAATG 2 cut(s) 858, 1650
Bse8I GATNNNNATC 1 cut(s) 1230
BseBI CCWGG 1 cut(s) 984
BseDI CCNNGG 6 cut(s) 12, 598, 619, 762, 929, 1566
BseGI GGATG 5 cut(s) 22, 120, 457, 1096, 1776
BseJI GATNNNNATC 1 cut(s) 1230
BseLI CCNNNNNNNGG 4 cut(s) 379, 475, 626, 1222
BseMI GCAATG 2 cut(s) 858, 1650
BseMII CTCAG 4 cut(s) 461, 968, 1235, 1356
BseNI ACTGG 5 cut(s) 998, 1037, 1084, 1637, 1913
BseRI GAGGAG 1 cut(s) 849
BseXI GCAGC 5 cut(s) 183, 444, 1477, 1507, 1510
BsgI GTGCAG 1 cut(s) 599
Bsh1236I CGCG 3 cut(s) 386, 788, 1895
Bsh1285I CGRYCG 1 cut(s) 607
BshFI GGCC 7 cut(s) 156, 511, 936, 982, 1008, 1327, 1759
BshNI GGYRCC 2 cut(s) 478, 647
BsiEI CGRYCG 1 cut(s) 607
BsiHKCI CYCGRG 2 cut(s) 762, 827
BsiSI CCGG 9 cut(s) 476, 493, 621, 763, 1005, 1277, 1328, 1470, 1925
BslFI GGGAC 1 cut(s) 745
BslI CCNNNNNNNGG 4 cut(s) 379, 475, 626, 1222
BsmAI GTCTC 5 cut(s) 789, 1250, 1327, 1703, 1776
BsmBI CGTCTC 1 cut(s) 1776
BsmFI GGGAC 1 cut(s) 745
BsmI GAATGC 1 cut(s) 1504
BsnI GGCC 7 cut(s) 156, 511, 936, 982, 1008, 1327, 1759
BsoBI CYCGRG 2 cut(s) 762, 827
BspANI GGCC 7 cut(s) 156, 511, 936, 982, 1008, 1327, 1759
BspCNI CTCAG 4 cut(s) 460, 969, 1234, 1355
BspFNI CGCG 3 cut(s) 386, 788, 1895
BspHI TCATGA 2 cut(s) 1551, 1870
BspLI GGNNCC 6 cut(s) 480, 631, 649, 935, 1227, 1563
BspPI GGATC 4 cut(s) 387, 1220, 1233, 1627
BspT107I GGYRCC 2 cut(s) 478, 647
BsrBI CCGCTC 1 cut(s) 1922
BsrDI GCAATG 2 cut(s) 858, 1650
BsrI ACTGG 5 cut(s) 998, 1037, 1084, 1637, 1913
BssECI CCNNGG 6 cut(s) 12, 598, 619, 762, 929, 1566
BssNI GRCGYC 3 cut(s) 351, 648, 855
BssT1I CCWWGG 2 cut(s) 598, 929
Bst2UI CCWGG 1 cut(s) 984
Bst4CI ACNGT 7 cut(s) 362, 440, 565, 608, 1372, 1447, 1690
Bst6I CTCTTC 1 cut(s) 131
BstACI GRCGYC 3 cut(s) 351, 648, 855
BstAPI GCANNNNNTGC 1 cut(s) 1212
BstC8I GCNNGC 1 cut(s) 1194
BstDEI CTNAG 5 cut(s) 447, 977, 1221, 1342, 1754
BstDSI CCRYGG 1 cut(s) 1566
BstF5I GGATG 5 cut(s) 22, 120, 457, 1096, 1776
BstFNI CGCG 3 cut(s) 386, 788, 1895
BstH2I RGCGCY 1 cut(s) 651
BstHHI GCGC 3 cut(s) 650, 790, 1465
BstMAI GTCTC 5 cut(s) 789, 1250, 1327, 1703, 1776
BstMCI CGRYCG 1 cut(s) 607
BstMWI GCNNNNNNNGC 3 cut(s) 536, 918, 1212
BstNI CCWGG 1 cut(s) 984
BstSCI CCNGG 5 cut(s) 619, 761, 762, 982, 1468
BstUI CGCG 3 cut(s) 386, 788, 1895
BstV1I GCAGC 5 cut(s) 183, 444, 1477, 1507, 1510
BstV2I GAAGAC 2 cut(s) 1065, 1731
BstX2I RGATCY 2 cut(s) 523, 1225
BstXI CCANNNNNNTGG 2 cut(s) 944, 1020
BstYI RGATCY 2 cut(s) 523, 1225
BsuRI GGCC 7 cut(s) 156, 511, 936, 982, 1008, 1327, 1759
BtgI CCRYGG 1 cut(s) 1566
BtrI CACGTC 1 cut(s) 1573
BtsCI GGATG 5 cut(s) 22, 120, 457, 1096, 1776
BtsI GCAGTG 1 cut(s) 907
BtsIMutI CAGTG 7 cut(s) 367, 436, 570, 907, 1350, 1452, 1695
Cac8I GCNNGC 1 cut(s) 1194
CciI TCATGA 2 cut(s) 1551, 1870
CfoI GCGC 3 cut(s) 650, 790, 1465
Cfr13I GGNCC 6 cut(s) 16, 510, 708, 934, 1007, 1865
Cfr9I CCCGGG 1 cut(s) 762
CpoI CGGWCCG 1 cut(s) 708
CseI GACGC 3 cut(s) 392, 844, 852
Csp6I GTAC 7 cut(s) 358, 367, 535, 839, 991, 1243, 1906
CspCI CAANNNNNGTGG 2 cut(s) 1814, 1849
CspI CGGWCCG 1 cut(s) 708
CviQI GTAC 7 cut(s) 358, 367, 535, 839, 991, 1243, 1906
DdeI CTNAG 5 cut(s) 447, 977, 1221, 1342, 1754
DinI GGCGCC 1 cut(s) 649
EaeI YGGCCR 1 cut(s) 1325
Eam1104I CTCTTC 1 cut(s) 131
EarI CTCTTC 1 cut(s) 131
EciI GGCGGA 1 cut(s) 193
Eco130I CCWWGG 2 cut(s) 598, 929
Eco147I AGGCCT 1 cut(s) 982
Eco47I GGWCC 3 cut(s) 16, 708, 1865
Eco57I CTGAAG 1 cut(s) 1410
Eco88I CYCGRG 2 cut(s) 762, 827
EcoRI GAATTC 1 cut(s) 1749
EcoRII CCWGG 1 cut(s) 982
EcoT14I CCWWGG 2 cut(s) 598, 929
EgeI GGCGCC 1 cut(s) 649
EheI GGCGCC 1 cut(s) 649
ErhI CCWWGG 2 cut(s) 598, 929
Esp3I CGTCTC 1 cut(s) 1776
FalI AAGNNNNNCTT 4 cut(s) 380, 412, 862, 894
FaqI GGGAC 1 cut(s) 745
FauI CCCGC 3 cut(s) 190, 850, 1927
FbaI TGATCA 4 cut(s) 681, 1231, 1773, 1852
FblI GTMKAC 1 cut(s) 1649
Fnu4HI GCNGC 6 cut(s) 172, 458, 1349, 1466, 1521, 1524
FokI GGATG 5 cut(s) 9, 107, 464, 1103, 1763
Fsp4HI GCNGC 6 cut(s) 172, 458, 1349, 1466, 1521, 1524
FspBI CTAG 7 cut(s) 269, 738, 888, 1101, 1287, 1529, 1736
FspI TGCGCA 1 cut(s) 1464
GlaI GCGC 3 cut(s) 649, 789, 1464
GluI GCNGC 6 cut(s) 172, 458, 1349, 1466, 1521, 1524
HaeII RGCGCY 1 cut(s) 651
HaeIII GGCC 7 cut(s) 156, 511, 936, 982, 1008, 1327, 1759
HapII CCGG 9 cut(s) 476, 493, 621, 763, 1005, 1277, 1328, 1470, 1925
HgaI GACGC 3 cut(s) 392, 844, 852
HhaI GCGC 3 cut(s) 650, 790, 1465
Hin1I GRCGYC 3 cut(s) 351, 648, 855
Hin6I GCGC 3 cut(s) 648, 788, 1463
HinP1I GCGC 3 cut(s) 648, 788, 1463
HincII GTYRAC 2 cut(s) 561, 1582
HindII GTYRAC 2 cut(s) 561, 1582
HindIII AAGCTT 2 cut(s) 1190, 1236
HinfI GANTC 5 cut(s) 997, 1201, 1267, 1406, 1593
HpaI GTTAAC 1 cut(s) 1582
HpaII CCGG 9 cut(s) 476, 493, 621, 763, 1005, 1277, 1328, 1470, 1925
HphI GGTGA 3 cut(s) 268, 1813, 1909
Hpy166II GTNNAC 8 cut(s) 369, 504, 561, 952, 1245, 1570, 1582, 1650
Hpy188I TCNGA 8 cut(s) 13, 642, 708, 978, 1411, 1592, 1619, 1781
Hpy8I GTNNAC 8 cut(s) 369, 504, 561, 952, 1245, 1570, 1582, 1650
Hpy99I CGWCG 4 cut(s) 353, 868, 1139, 1790
HpyAV CCTTC 8 cut(s) 565, 894, 1030, 1123, 1369, 1467, 1496, 1570
HpyCH4III ACNGT 7 cut(s) 362, 440, 565, 608, 1372, 1447, 1690
HpyCH4IV ACGT 6 cut(s) 351, 500, 1137, 1572, 1606, 1788
HpyCH4V TGCA 4 cut(s) 135, 580, 1142, 1176
HpyF10VI GCNNNNNNNGC 3 cut(s) 536, 918, 1212
HpyF3I CTNAG 5 cut(s) 447, 977, 1221, 1342, 1754
HpySE526I ACGT 6 cut(s) 351, 500, 1137, 1572, 1606, 1788
Hsp92I GRCGYC 3 cut(s) 351, 648, 855
HspAI GCGC 3 cut(s) 648, 788, 1463
KasI GGCGCC 1 cut(s) 647
Ksp22I TGATCA 4 cut(s) 681, 1231, 1773, 1852
KspAI GTTAAC 1 cut(s) 1582
LmnI GCTCC 4 cut(s) 746, 1411, 1921, 1927
Lsp1109I GCAGC 5 cut(s) 183, 444, 1477, 1507, 1510
LweI GCATC 2 cut(s) 144, 643
MaeI CTAG 7 cut(s) 269, 738, 888, 1101, 1287, 1529, 1736
MaeII ACGT 6 cut(s) 351, 500, 1137, 1572, 1606, 1788
MaeIII GTNAC 3 cut(s) 224, 1381, 1429
MbiI CCGCTC 1 cut(s) 1922
MboII GAAGA 9 cut(s) 118, 130, 1036, 1070, 1082, 1304, 1403, 1736, 1816
MflI RGATCY 2 cut(s) 523, 1225
MluCI AATT 7 cut(s) 401, 583, 818, 1143, 1356, 1749, 1845
Mly113I GGCGCC 1 cut(s) 648
MlyI GAGTC 2 cut(s) 1261, 1602
MmeI TCCRAC 1 cut(s) 1233
MroXI GAANNNNTTC 1 cut(s) 1395
MseI TTAA 3 cut(s) 612, 1169, 1581
MslI CAYNNNNRTG 1 cut(s) 1556
MspA1I CMGCKG 2 cut(s) 1020, 1388
MspI CCGG 9 cut(s) 476, 493, 621, 763, 1005, 1277, 1328, 1470, 1925
MspR9I CCNGG 5 cut(s) 621, 763, 764, 984, 1470
Mva1269I GAATGC 1 cut(s) 1504
MvaI CCWGG 1 cut(s) 984
MvnI CGCG 3 cut(s) 386, 788, 1895
MwoI GCNNNNNNNGC 3 cut(s) 536, 918, 1212
NarI GGCGCC 1 cut(s) 648
NciI CCSGG 4 cut(s) 621, 763, 764, 1470
NlaIV GGNNCC 6 cut(s) 480, 631, 649, 935, 1227, 1563
NmeAIII GCCGAG 1 cut(s) 1377
NmuCI GTSAC 1 cut(s) 1381
NsbI TGCGCA 1 cut(s) 1464
PaeR7I CTCGAG 1 cut(s) 827
PagI TCATGA 2 cut(s) 1551, 1870
PceI AGGCCT 1 cut(s) 982
PctI GAATGC 1 cut(s) 1504
PdmI GAANNNNTTC 1 cut(s) 1395
PfeI GAWTC 3 cut(s) 997, 1201, 1406
PkrI GCNGC 6 cut(s) 173, 459, 1350, 1467, 1522, 1525
PleI GAGTC 2 cut(s) 1261, 1601
PluTI GGCGCC 1 cut(s) 651
PpsI GAGTC 2 cut(s) 1261, 1601
PsiI TTATAA 1 cut(s) 1440
Psp6I CCWGG 1 cut(s) 982
PspGI CCWGG 1 cut(s) 982
PspN4I GGNNCC 6 cut(s) 480, 631, 649, 935, 1227, 1563
PspPI GGNCC 6 cut(s) 16, 510, 708, 934, 1007, 1865
PsuI RGATCY 2 cut(s) 523, 1225
PvuII CAGCTG 1 cut(s) 1388
RsaI GTAC 7 cut(s) 359, 368, 536, 840, 992, 1244, 1907
RsaNI GTAC 7 cut(s) 358, 367, 535, 839, 991, 1243, 1906
RseI CAYNNNNRTG 1 cut(s) 1556
Rsr2I CGGWCCG 1 cut(s) 708
RsrII CGGWCCG 1 cut(s) 708
SaqAI TTAA 3 cut(s) 612, 1169, 1581
SatI GCNGC 6 cut(s) 172, 458, 1349, 1466, 1521, 1524
Sau96I GGNCC 6 cut(s) 16, 510, 708, 934, 1007, 1865
ScaI AGTACT 1 cut(s) 1907
SchI GAGTC 2 cut(s) 1261, 1602
ScrFI CCNGG 5 cut(s) 621, 763, 764, 984, 1470
SfaNI GCATC 2 cut(s) 144, 643
SfoI GGCGCC 1 cut(s) 649
Sfr274I CTCGAG 1 cut(s) 827
SinI GGWCC 3 cut(s) 16, 708, 1865
SlaI CTCGAG 1 cut(s) 827
SmaI CCCGGG 1 cut(s) 764
SmiMI CAYNNNNRTG 1 cut(s) 1556
SmlI CTYRAG 3 cut(s) 461, 689, 827
SmoI CTYRAG 3 cut(s) 461, 689, 827
Sse9I AATT 7 cut(s) 401, 583, 818, 1143, 1356, 1749, 1845
SseBI AGGCCT 1 cut(s) 982
SspDI GGCGCC 1 cut(s) 647
SspMI CTAG 7 cut(s) 269, 738, 888, 1101, 1287, 1529, 1736
StuI AGGCCT 1 cut(s) 982
StyD4I CCNGG 5 cut(s) 619, 761, 762, 982, 1468
StyI CCWWGG 2 cut(s) 598, 929
TaaI ACNGT 7 cut(s) 362, 440, 565, 608, 1372, 1447, 1690
TaiI ACGT 6 cut(s) 354, 503, 1140, 1575, 1609, 1791
TaqI TCGA 5 cut(s) 41, 405, 828, 866, 1821
TasI AATT 7 cut(s) 401, 583, 818, 1143, 1356, 1749, 1845
TatI WGTACW 3 cut(s) 357, 366, 1905
TauI GCSGC 1 cut(s) 1351
TfiI GAWTC 3 cut(s) 997, 1201, 1406
Tru1I TTAA 3 cut(s) 612, 1169, 1581
Tru9I TTAA 3 cut(s) 612, 1169, 1581
TscAI CASTG 7 cut(s) 367, 443, 570, 914, 1350, 1452, 1695
TseFI GTSAC 1 cut(s) 1381
TseI GCWGC 5 cut(s) 171, 457, 1465, 1520, 1523
Tsp45I GTSAC 1 cut(s) 1381
TspDTI ATGAA 6 cut(s) 819, 1196, 1511, 1568, 1617, 1667
TspGWI ACGGA 2 cut(s) 974, 1878
TspMI CCCGGG 1 cut(s) 762
TspRI CASTG 7 cut(s) 367, 443, 570, 914, 1350, 1452, 1695
VpaK11BI GGWCC 3 cut(s) 16, 708, 1865
XapI RAATTY 2 cut(s) 401, 1749
XbaI TCTAGA 1 cut(s) 887
XcmI CCANNNNNNNNNTGG 1 cut(s) 1017
XhoI CTCGAG 1 cut(s) 827
XmaI CCCGGG 1 cut(s) 762
XmiI GTMKAC 1 cut(s) 1649
XmnI GAANNNNTTC 1 cut(s) 1395
XspI CTAG 7 cut(s) 269, 738, 888, 1101, 1287, 1529, 1736
ZraI GACGTC 1 cut(s) 352
ZrmI AGTACT 1 cut(s) 1907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.