MD07G1265000.v1.1

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013803
Physical Location & Seq
Forward (+)
33382301 .. 33383209
909 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1265000.v1.1.491

Sequence Viewer

Length: 564 bp
ATGATCCTGATACGGCTTGGTATGTACACAAAGTTGGAGACTTGTTTGACCCCATTGCCAGAAGTTTCAGATAATGAAGAAGTTGCAGGAGGAAAATTGGCAAACTGGCCACATAGGCTCAATGCAATCCCGCCAAGGATTAGCAAGGGAGCTGTGGAAGGTGTGACGGCTGATGTTTTTAAGCAAGATTCGGAGCTGTGGAAGAAGAGGGTTTCGTATTATGAATCGGTGAATAATCAGCTCGGAAACCCTGGAAGGTACAAAAACATTTTGGACATGAATGCACAACTGGGTGGATTTGCTGCTGCCGTAGTTGATCTGCCTGTCTGGGTCATGAATGTCGTTCCGGTGGAAGTTAAGGCTAACACACTAGGAACCATCTACGAACGAGGACTGATTGGAACATATCAGAATTGGTGTGAAGCAATGTCTACATATCCAAGAACTTATGACTTCATTCATGCTGATTCAGTGTTTAGCCTGTATCAGGATAGATGCAATGCAGATGACATTTTGCTGGAGATGGACAGAATTCTGAGGCTAGATGGGAGCGTGATCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

188

Amino Acids

21.02

Weight (kDa)

5.07

Isoelectric Point (pI)

31.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 9 - 187 2.9e-73 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 431
AciI CCGC 1 cut(s) 131
AcoI YGGCCR 1 cut(s) 107
AcsI RAATTY 1 cut(s) 531
AfaI GTAC 2 cut(s) 26, 260
AfiI CCNNNNNNNGG 1 cut(s) 487
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 3 cut(s) 152, 196, 241
AluI AGCT 3 cut(s) 152, 196, 241
Alw26I GTCTC 1 cut(s) 32
AoxI GGCC 1 cut(s) 107
ApeKI GCWGC 2 cut(s) 302, 305
ApoI RAATTY 1 cut(s) 531
AsuHPI GGTGA 1 cut(s) 241
BalI TGGCCA 1 cut(s) 109
BbvI GCAGC 2 cut(s) 289, 292
BccI CCATC 3 cut(s) 386, 517, 539
BceAI ACGGC 3 cut(s) 29, 183, 293
BciT130I CCWGG 1 cut(s) 252
BclI TGATCA 1 cut(s) 555
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 2 cut(s) 371, 542
BglI GCCNNNNNGGC 1 cut(s) 115
BisI GCNGC 2 cut(s) 303, 306
BlsI GCNGC 2 cut(s) 304, 307
Bme1390I CCNGG 1 cut(s) 252
BmiI GGNNCC 1 cut(s) 376
BmrFI CCNGG 1 cut(s) 252
BmrI ACTGGG 1 cut(s) 299
BmsI GCATC 1 cut(s) 485
BmuI ACTGGG 1 cut(s) 299
BpmI CTGGAG 1 cut(s) 539
BsaJI CCNNGG 2 cut(s) 134, 250
BsaWI WCCGGW 1 cut(s) 346
Bsc4I CCNNNNNNNGG 1 cut(s) 487
Bse1I ACTGG 2 cut(s) 110, 294
Bse3DI GCAATG 3 cut(s) 53, 432, 505
BseBI CCWGG 1 cut(s) 252
BseDI CCNNGG 2 cut(s) 134, 250
BseLI CCNNNNNNNGG 1 cut(s) 487
BseMI GCAATG 3 cut(s) 53, 432, 505
BseMII CTCAG 1 cut(s) 527
BseNI ACTGG 2 cut(s) 110, 294
BseXI GCAGC 2 cut(s) 289, 292
BshFI GGCC 1 cut(s) 109
BsiSI CCGG 1 cut(s) 347
BslI CCNNNNNNNGG 1 cut(s) 487
BsmAI GTCTC 1 cut(s) 32
BsmI GAATGC 1 cut(s) 286
BsnI GGCC 1 cut(s) 109
Bsp1407I TGTACA 1 cut(s) 24
Bsp143I GATC 3 cut(s) 3, 316, 555
BspACI CCGC 1 cut(s) 131
BspANI GGCC 1 cut(s) 109
BspCNI CTCAG 1 cut(s) 528
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 1 cut(s) 376
BsrDI GCAATG 3 cut(s) 53, 432, 505
BsrGI TGTACA 1 cut(s) 24
BsrI ACTGG 2 cut(s) 110, 294
BssECI CCNNGG 2 cut(s) 134, 250
BssMI GATC 3 cut(s) 3, 316, 555
BssT1I CCWWGG 1 cut(s) 134
Bst2UI CCWGG 1 cut(s) 252
Bst6I CTCTTC 1 cut(s) 200
BstAUI TGTACA 1 cut(s) 24
BstDEI CTNAG 1 cut(s) 536
BstENI CCTNNNNNAGG 1 cut(s) 485
BstKTI GATC 3 cut(s) 6, 319, 558
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 3 cut(s) 3, 316, 555
BstMWI GCNNNNNNNGC 1 cut(s) 115
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 1 cut(s) 250
BstV1I GCAGC 2 cut(s) 289, 292
BsuRI GGCC 1 cut(s) 109
BtsIMutI CAGTG 1 cut(s) 477
CciI TCATGA 1 cut(s) 333
Csp6I GTAC 2 cut(s) 25, 259
CviAII CATG 3 cut(s) 277, 334, 461
CviQI GTAC 2 cut(s) 25, 259
DdeI CTNAG 1 cut(s) 536
DpnI GATC 3 cut(s) 5, 318, 557
DpnII GATC 3 cut(s) 3, 316, 555
EaeI YGGCCR 1 cut(s) 107
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 134
EcoNI CCTNNNNNAGG 1 cut(s) 485
EcoRI GAATTC 1 cut(s) 531
EcoRII CCWGG 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 134
ErhI CCWWGG 1 cut(s) 134
FaeI CATG 3 cut(s) 280, 337, 464
FaiI YATR 9 cut(s) 23, 114, 222, 278, 335, 406, 436, 450, 462
FatI CATG 3 cut(s) 276, 333, 460
FauI CCCGC 1 cut(s) 138
FbaI TGATCA 1 cut(s) 555
FblI GTMKAC 1 cut(s) 431
Fnu4HI GCNGC 2 cut(s) 303, 306
Fsp4HI GCNGC 2 cut(s) 303, 306
FspBI CTAG 2 cut(s) 371, 542
GluI GCNGC 2 cut(s) 303, 306
GsuI CTGGAG 1 cut(s) 539
HaeIII GGCC 1 cut(s) 109
HapII CCGG 1 cut(s) 347
Hin1II CATG 3 cut(s) 280, 337, 464
HinfI GANTC 3 cut(s) 188, 224, 467
HpaII CCGG 1 cut(s) 347
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 2 cut(s) 27, 432
Hpy188I TCNGA 5 cut(s) 70, 193, 245, 411, 537
Hpy188III TCNNGA 3 cut(s) 7, 334, 488
Hpy8I GTNNAC 2 cut(s) 27, 432
HpyAV CCTTC 2 cut(s) 152, 249
HpyCH4V TGCA 5 cut(s) 86, 125, 284, 498, 503
HpyF10VI GCNNNNNNNGC 1 cut(s) 115
HpyF3I CTNAG 1 cut(s) 536
Hsp92II CATG 3 cut(s) 280, 337, 464
Ksp22I TGATCA 1 cut(s) 555
Kzo9I GATC 3 cut(s) 3, 316, 555
LmnI GCTCC 3 cut(s) 149, 193, 549
Lsp1109I GCAGC 2 cut(s) 289, 292
LweI GCATC 1 cut(s) 485
MaeI CTAG 2 cut(s) 371, 542
MaeIII GTNAC 1 cut(s) 163
MalI GATC 3 cut(s) 5, 318, 557
MboI GATC 3 cut(s) 3, 316, 555
MboII GAAGA 3 cut(s) 89, 214, 217
MlsI TGGCCA 1 cut(s) 109
MluCI AATT 3 cut(s) 95, 412, 531
MluNI TGGCCA 1 cut(s) 109
MmeI TCCRAC 1 cut(s) 15
MnlI CCTC 4 cut(s) 83, 201, 383, 531
Mox20I TGGCCA 1 cut(s) 109
MscI TGGCCA 1 cut(s) 109
MseI TTAA 2 cut(s) 180, 357
Msp20I TGGCCA 1 cut(s) 109
MspI CCGG 1 cut(s) 347
MspR9I CCNGG 1 cut(s) 252
Mva1269I GAATGC 1 cut(s) 286
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 1 cut(s) 115
NdeII GATC 3 cut(s) 3, 316, 555
NlaIII CATG 3 cut(s) 280, 337, 464
NlaIV GGNNCC 1 cut(s) 376
NmuCI GTSAC 1 cut(s) 163
PagI TCATGA 1 cut(s) 333
PctI GAATGC 1 cut(s) 286
PfeI GAWTC 3 cut(s) 188, 224, 467
PkrI GCNGC 2 cut(s) 304, 307
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 1 cut(s) 376
RsaI GTAC 2 cut(s) 26, 260
RsaNI GTAC 2 cut(s) 25, 259
SaqAI TTAA 2 cut(s) 180, 357
SatI GCNGC 2 cut(s) 303, 306
Sau3AI GATC 3 cut(s) 3, 316, 555
ScrFI CCNGG 1 cut(s) 252
SetI ASST 5 cut(s) 154, 163, 198, 243, 260
SfaNI GCATC 1 cut(s) 485
Sse9I AATT 3 cut(s) 95, 412, 531
SsiI CCGC 1 cut(s) 131
SspMI CTAG 2 cut(s) 371, 542
StyD4I CCNGG 1 cut(s) 250
StyI CCWWGG 1 cut(s) 134
TasI AATT 3 cut(s) 95, 412, 531
TatI WGTACW 1 cut(s) 24
TfiI GAWTC 3 cut(s) 188, 224, 467
Tru1I TTAA 2 cut(s) 180, 357
Tru9I TTAA 2 cut(s) 180, 357
TscAI CASTG 1 cut(s) 477
TseFI GTSAC 1 cut(s) 163
TseI GCWGC 2 cut(s) 302, 305
Tsp45I GTSAC 1 cut(s) 163
TspDTI ATGAA 6 cut(s) 90, 237, 293, 350, 445, 449
TspRI CASTG 1 cut(s) 477
XagI CCTNNNNNAGG 1 cut(s) 485
XapI RAATTY 1 cut(s) 531
XmiI GTMKAC 1 cut(s) 431
XspI CTAG 2 cut(s) 371, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.