MD01G1212900.v1.1

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0019771
Physical Location & Seq
Reverse (-)
30632228 .. 30633136
909 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1212900.v1.1.491

Sequence Viewer

Length: 894 bp
ATGGCAACCAAAACACAAACCCTAGCCCTAACGCTGTTCCTCTTGATTCTCATTTCTTCATGCAAGTCCTCTCAAGCCGCCGGAATCGCAACGTATTGGGGACAAAACGGCAACGAAGGAACCCTAGTAGATGCTTGCAACTCAGGCAACTACCAGTTTGTTAACATAGCTTTCCTCATGACTTTCGGAAACAACCAGGTCCCTGCCCTCAACCTCGCCGGACACTGCGACCCCAGCAGTAGTACTTGCACGGGGCTGAGCGCCGACATCAGAGCTTGCCAATCCCAAAACATAAAAGTCCTCCTCTCGATTGGAGGGGCTGTCGGAAGTTACAATCTCACTTCAGCTGATGATGCAAGGCAAGTTGCTGATTACATCTGGAACAACTTCCTAGGTGGTCAGTCAGCATCGCGCCCGCTTGGGGACGCTGTTTTGGACGGCGTTGATTTCGCCATTGTTATAGGTGGTGGGCAGTTCTATGATGAGCTCGCCAGGTTACTCAACGGGCACAACACACAGGCAAAAACGGTCTATTTAGCCGCAGCTCCACAATGTCCAATCCCGGATGCTCACCTAGACGGCGCAATCCAAACTGGCTTATTTGACTACGTTTGGGTTCAGTTCTACAACAACCCCCCATGCCAGTATTCAGACGGTAATGCCAACGCTCTTTTGAGCAGTTGGAACCAATGGGCCTCGGTTCCGGCCAGCGAGGTGTTCTTGGGTTTACCAGCAGCTCCTGAGGCCGCTCCGAGCGGCGGATTTATTCCTGCTGACGCTCTCAAGTCACAAGTTCTTCCAACGATTAAGAATTCGCCCAAGTACGGAGGAGTTATGCTCTGGAGCAGGCAGTATGACAACGGTTACAGTGCATCCATTATGGACAACATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

298

Amino Acids

31.42

Weight (kDa)

4.53

Isoelectric Point (pI)

38.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 29 - 245 2.4e-17 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019771)

Species Orthologous Gene IDs
malus_domestica MD01G1212900.v1.1 MD01G1213100.v1.1 MD01G1213300.v1.1 MD07G1282900.v1.1
prunus_persica Prupe.2G305200_v2.0.a1
pyrus_communis pycom01g22400 pycom01g22420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 749, 756
AccII CGCG 1 cut(s) 412
AciI CCGC 6 cut(s) 78, 416, 540, 747, 756, 759
AcoI YGGCCR 1 cut(s) 705
AcsI RAATTY 1 cut(s) 811
AcuI CTGAAG 1 cut(s) 327
AfaI GTAC 2 cut(s) 244, 824
AfiI CCNNNNNNNGG 3 cut(s) 421, 758, 824
AjnI CCWGG 2 cut(s) 195, 491
AluBI AGCT 6 cut(s) 170, 275, 347, 487, 545, 737
AluI AGCT 6 cut(s) 170, 275, 347, 487, 545, 737
Alw21I GWGCWC 1 cut(s) 489
AlwNI CAGNNNCTG 1 cut(s) 740
AoxI GGCC 3 cut(s) 693, 705, 744
ApeKI GCWGC 2 cut(s) 542, 734
ApoI RAATTY 1 cut(s) 811
Asp700I GAANNNNTTC 1 cut(s) 386
AspA2I CCTAGG 1 cut(s) 391
AspLEI GCGC 3 cut(s) 263, 414, 584
AspS9I GGNCC 2 cut(s) 199, 693
AsuC2I CCSGG 1 cut(s) 563
AsuHPI GGTGA 1 cut(s) 563
AvaII GGWCC 1 cut(s) 199
AvrII CCTAGG 1 cut(s) 391
AxyI CCTNAGG 1 cut(s) 741
BaeGI GKGCMC 1 cut(s) 510
BanII GRGCYC 1 cut(s) 489
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 2 cut(s) 554, 746
BceAI ACGGC 3 cut(s) 124, 454, 595
BciT130I CCWGG 2 cut(s) 197, 493
BcnI CCSGG 1 cut(s) 563
BfaI CTAG 4 cut(s) 23, 125, 392, 575
BfoI RGCGCY 1 cut(s) 264
BisI GCNGC 6 cut(s) 78, 540, 543, 735, 747, 757
BlnI CCTAGG 1 cut(s) 391
BlpI GCTNAGC 1 cut(s) 257
BlsI GCNGC 6 cut(s) 79, 541, 544, 736, 748, 758
BmcAI AGTACT 1 cut(s) 244
Bme1390I CCNGG 3 cut(s) 197, 493, 563
Bme18I GGWCC 1 cut(s) 199
BmgT120I GGNCC 2 cut(s) 199, 693
BmiI GGNNCC 4 cut(s) 121, 201, 686, 702
BmrFI CCNGG 3 cut(s) 197, 493, 563
BmsI GCATC 5 cut(s) 121, 343, 416, 556, 881
BplI GAGNNNNNCTC 2 cut(s) 822, 854
BpmI CTGGAG 1 cut(s) 862
Bpu1102I GCTNAGC 1 cut(s) 257
BpuEI CTTGAG 2 cut(s) 57, 767
BpuMI CCSGG 1 cut(s) 563
BsaJI CCNNGG 2 cut(s) 391, 696
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 3 cut(s) 421, 758, 824
Bse1I ACTGG 3 cut(s) 154, 598, 643
Bse21I CCTNAGG 1 cut(s) 741
BseBI CCWGG 2 cut(s) 197, 493
BseDI CCNNGG 2 cut(s) 391, 696
BseGI GGATG 2 cut(s) 571, 872
BseLI CCNNNNNNNGG 3 cut(s) 421, 758, 824
BseMII CTCAG 3 cut(s) 156, 248, 732
BseNI ACTGG 3 cut(s) 154, 598, 643
BseRI GAGGAG 2 cut(s) 293, 843
BseSI GKGCMC 1 cut(s) 510
BseXI GCAGC 2 cut(s) 554, 746
BseYI CCCAGC 1 cut(s) 233
Bsh1236I CGCG 1 cut(s) 412
BshFI GGCC 3 cut(s) 695, 707, 746
BsiHKAI GWGCWC 1 cut(s) 489
BsiSI CCGG 4 cut(s) 81, 219, 563, 704
BslFI GGGAC 3 cut(s) 114, 185, 437
BslI CCNNNNNNNGG 3 cut(s) 421, 758, 824
BsmFI GGGAC 3 cut(s) 114, 185, 437
BsnI GGCC 3 cut(s) 695, 707, 746
Bsp1286I GDGCHC 2 cut(s) 489, 510
Bsp1720I GCTNAGC 1 cut(s) 257
BspACI CCGC 6 cut(s) 78, 416, 540, 747, 756, 759
BspANI GGCC 3 cut(s) 695, 707, 746
BspCNI CTCAG 3 cut(s) 155, 249, 733
BspFNI CGCG 1 cut(s) 412
BspHI TCATGA 1 cut(s) 177
BspLI GGNNCC 4 cut(s) 121, 201, 686, 702
BsrBI CCGCTC 2 cut(s) 749, 756
BsrI ACTGG 3 cut(s) 154, 598, 643
BssECI CCNNGG 2 cut(s) 391, 696
BssT1I CCWWGG 1 cut(s) 391
Bst2UI CCWGG 2 cut(s) 197, 493
Bst4CI ACNGT 4 cut(s) 529, 656, 863, 869
BstC8I GCNNGC 6 cut(s) 136, 277, 416, 489, 709, 848
BstDEI CTNAG 3 cut(s) 142, 257, 741
BstF5I GGATG 2 cut(s) 571, 872
BstFNI CGCG 1 cut(s) 412
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 3 cut(s) 263, 414, 584
BstMWI GCNNNNNNNGC 5 cut(s) 86, 144, 234, 353, 743
BstNI CCWGG 2 cut(s) 197, 493
BstSCI CCNGG 3 cut(s) 195, 491, 561
BstSLI GKGCMC 1 cut(s) 510
BstUI CGCG 1 cut(s) 412
BstV1I GCAGC 2 cut(s) 554, 746
Bsu36I CCTNAGG 1 cut(s) 741
BsuRI GGCC 3 cut(s) 695, 707, 746
BtgZI GCGATG 1 cut(s) 393
BtsCI GGATG 2 cut(s) 571, 872
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 2 cut(s) 223, 874
Cac8I GCNNGC 6 cut(s) 136, 277, 416, 489, 709, 848
CaiI CAGNNNCTG 1 cut(s) 740
CciI TCATGA 1 cut(s) 177
CfoI GCGC 3 cut(s) 263, 414, 584
Cfr13I GGNCC 2 cut(s) 199, 693
CseI GACGC 2 cut(s) 434, 785
CsiI ACCWGGT 1 cut(s) 195
Csp6I GTAC 2 cut(s) 243, 823
CviAII CATG 3 cut(s) 60, 178, 639
CviQI GTAC 2 cut(s) 243, 823
DdeI CTNAG 3 cut(s) 142, 257, 741
EaeI YGGCCR 1 cut(s) 705
EciI GGCGGA 1 cut(s) 774
Ecl136II GAGCTC 1 cut(s) 487
Eco130I CCWWGG 1 cut(s) 391
Eco24I GRGCYC 1 cut(s) 489
Eco47I GGWCC 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 487
Eco57I CTGAAG 1 cut(s) 327
Eco81I CCTNAGG 1 cut(s) 741
EcoICRI GAGCTC 1 cut(s) 487
EcoO109I RGGNCCY 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 811
EcoRII CCWGG 2 cut(s) 195, 491
EcoT14I CCWWGG 1 cut(s) 391
EcoT38I GRGCYC 1 cut(s) 489
ErhI CCWWGG 1 cut(s) 391
FaeI CATG 3 cut(s) 63, 181, 642
FaqI GGGAC 3 cut(s) 114, 185, 437
FatI CATG 3 cut(s) 59, 177, 638
FauI CCCGC 1 cut(s) 423
Fnu4HI GCNGC 6 cut(s) 78, 540, 543, 735, 747, 757
FokI GGATG 2 cut(s) 578, 859
FriOI GRGCYC 1 cut(s) 489
Fsp4HI GCNGC 6 cut(s) 78, 540, 543, 735, 747, 757
FspBI CTAG 4 cut(s) 23, 125, 392, 575
GlaI GCGC 3 cut(s) 262, 413, 583
GluI GCNGC 6 cut(s) 78, 540, 543, 735, 747, 757
GsaI CCCAGC 1 cut(s) 237
GsuI CTGGAG 1 cut(s) 862
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 3 cut(s) 695, 707, 746
HapII CCGG 4 cut(s) 81, 219, 563, 704
HgaI GACGC 2 cut(s) 434, 785
HhaI GCGC 3 cut(s) 263, 414, 584
Hin1II CATG 3 cut(s) 63, 181, 642
Hin6I GCGC 3 cut(s) 261, 412, 582
HinP1I GCGC 3 cut(s) 261, 412, 582
HincII GTYRAC 1 cut(s) 163
HindII GTYRAC 1 cut(s) 163
HinfI GANTC 2 cut(s) 46, 84
HpaI GTTAAC 1 cut(s) 163
HpaII CCGG 4 cut(s) 81, 219, 563, 704
HphI GGTGA 1 cut(s) 563
Hpy166II GTNNAC 2 cut(s) 163, 728
Hpy188I TCNGA 5 cut(s) 188, 272, 326, 652, 753
Hpy188III TCNNGA 6 cut(s) 43, 178, 307, 379, 740, 841
Hpy8I GTNNAC 2 cut(s) 163, 728
HpyAV CCTTC 1 cut(s) 110
HpyCH4III ACNGT 4 cut(s) 529, 656, 863, 869
HpyCH4IV ACGT 2 cut(s) 92, 609
HpyCH4V TGCA 5 cut(s) 63, 138, 249, 356, 872
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 144, 234, 353, 743
HpyF3I CTNAG 3 cut(s) 142, 257, 741
HpySE526I ACGT 2 cut(s) 92, 609
Hsp92II CATG 3 cut(s) 63, 181, 642
HspAI GCGC 3 cut(s) 261, 412, 582
KspAI GTTAAC 1 cut(s) 163
LmnI GCTCC 4 cut(s) 550, 742, 754, 843
Lsp1109I GCAGC 2 cut(s) 554, 746
LweI GCATC 5 cut(s) 121, 343, 416, 556, 881
MabI ACCWGGT 1 cut(s) 195
MaeI CTAG 4 cut(s) 23, 125, 392, 575
MaeII ACGT 2 cut(s) 92, 609
MaeIII GTNAC 4 cut(s) 329, 495, 786, 863
MbiI CCGCTC 2 cut(s) 749, 756
MboII GAAGA 2 cut(s) 48, 788
MhlI GDGCHC 2 cut(s) 489, 510
MluCI AATT 1 cut(s) 811
MmeI TCCRAC 3 cut(s) 304, 662, 824
MroXI GAANNNNTTC 1 cut(s) 386
MseI TTAA 2 cut(s) 162, 807
MspA1I CMGCKG 1 cut(s) 347
MspI CCGG 4 cut(s) 81, 219, 563, 704
MspR9I CCNGG 3 cut(s) 197, 493, 563
MvaI CCWGG 2 cut(s) 197, 493
MvnI CGCG 1 cut(s) 412
MwoI GCNNNNNNNGC 5 cut(s) 86, 144, 234, 353, 743
NciI CCSGG 1 cut(s) 563
NlaIII CATG 3 cut(s) 63, 181, 642
NlaIV GGNNCC 4 cut(s) 121, 201, 686, 702
NmuCI GTSAC 1 cut(s) 786
PagI TCATGA 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 386
PfeI GAWTC 2 cut(s) 46, 84
PfoI TCCNGGA 1 cut(s) 561
PkrI GCNGC 6 cut(s) 79, 541, 544, 736, 748, 758
PpuMI RGGWCCY 1 cut(s) 199
Psp124BI GAGCTC 1 cut(s) 489
Psp5II RGGWCCY 1 cut(s) 199
Psp6I CCWGG 2 cut(s) 195, 491
PspFI CCCAGC 1 cut(s) 233
PspGI CCWGG 2 cut(s) 195, 491
PspN4I GGNNCC 4 cut(s) 121, 201, 686, 702
PspPI GGNCC 2 cut(s) 199, 693
PspPPI RGGWCCY 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 740
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 2 cut(s) 244, 824
RsaNI GTAC 2 cut(s) 243, 823
SacI GAGCTC 1 cut(s) 489
SaqAI TTAA 2 cut(s) 162, 807
SatI GCNGC 6 cut(s) 78, 540, 543, 735, 747, 757
Sau96I GGNCC 2 cut(s) 199, 693
ScaI AGTACT 1 cut(s) 244
ScrFI CCNGG 3 cut(s) 197, 493, 563
SduI GDGCHC 2 cut(s) 489, 510
SexAI ACCWGGT 1 cut(s) 195
SfaNI GCATC 5 cut(s) 121, 343, 416, 556, 881
SinI GGWCC 1 cut(s) 199
SmlI CTYRAG 2 cut(s) 72, 782
SmoI CTYRAG 2 cut(s) 72, 782
Sse9I AATT 1 cut(s) 811
SsiI CCGC 6 cut(s) 78, 416, 540, 747, 756, 759
SspMI CTAG 4 cut(s) 23, 125, 392, 575
SstI GAGCTC 1 cut(s) 489
StyD4I CCNGG 3 cut(s) 195, 491, 561
StyI CCWWGG 1 cut(s) 391
TaaI ACNGT 4 cut(s) 529, 656, 863, 869
TaiI ACGT 2 cut(s) 95, 612
TaqI TCGA 1 cut(s) 308
TasI AATT 1 cut(s) 811
TatI WGTACW 1 cut(s) 242
TauI GCSGC 4 cut(s) 80, 542, 749, 759
TfiI GAWTC 2 cut(s) 46, 84
Tru1I TTAA 2 cut(s) 162, 807
Tru9I TTAA 2 cut(s) 162, 807
TscAI CASTG 2 cut(s) 230, 874
TseFI GTSAC 1 cut(s) 786
TseI GCWGC 2 cut(s) 542, 734
Tsp45I GTSAC 1 cut(s) 786
TspDTI ATGAA 1 cut(s) 48
TspGWI ACGGA 1 cut(s) 840
TspRI CASTG 2 cut(s) 230, 874
VpaK11BI GGWCC 1 cut(s) 199
XapI RAATTY 1 cut(s) 811
XmaJI CCTAGG 1 cut(s) 391
XmnI GAANNNNTTC 1 cut(s) 386
XspI CTAG 4 cut(s) 23, 125, 392, 575
ZrmI AGTACT 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.