MD01G1213100.v1.1

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0019771
Physical Location & Seq
Reverse (-)
30637543 .. 30654886
17344 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1213100.v1.1.491

Sequence Viewer

Length: 1014 bp
ATGTCCCCTCCCAAGCACTTTGAGCAACAACCCAAAAAAACACATACAAAAATGGCATCCAAAACACAAACCCTAACCCTAACTCTGTCCCTCTTGATCCTCATTTCTTCATGCAAGTCCTCCCAAGCCGCCGGAATTGCGATCTATTGGGGCCAAAACGGCAACGAAGGAACCCTAGCAGATGCTTGCAACTCAGGCAACTACCAGTTTGTTAACATAGCTTTCCTCACTACTTTCGGAAACAACCAAACCCCTGTCCTAAACCTCGCCGGCCACTGCGACCCCCCTAGTGGTACTTGCACGGGGCTGAGTGCTGACATCAGAACCTGCCAATCAAAAAACATAAAAGTCCTCCTCTCGATTGGAGGGGGCGCCGGAAGTTACTCTCTCACTTCAGCTGATGATGCAAGGCAAGTTGCTGATTACATCTGGAACAACTTCTTAGGTGGTCAGTCCGCTTCGCGCCCGCTTGGGGACGCGGTTTTGGACGGCGTTGATTTCGACATTGAGGCGGGTGGTGCGCAGTTCTATGATGAGCTCGCCAGGTCACTCAACGGACACAACGCACAGGCAAAAACGGTCTATTTAGCCGCAGCTCCACAATGTCCGATCCCGGATGCTCACCTGGACGCCGCTATCCAAACCGGTTTATTTGACTACGTTTGGGTTCAGTTCTACAACAACCCCCCATGCCAGTATGCAGACGGTAATGCCGACGCTCTTTTAAGCAGTTGGAACCAATGGGCCTCGGTTCCGGCCACCCAGTATGCAGACGGTAATGCCGACGCTCTTTTGAGCAGTTGGAACCAATGGGCCTCGGTTCCGGCCACCCAGGTGTTCATGGGGTTACCGGCAGCTCCTGAGGCCGCTCCGAGCGGCGGATTTATTCCTGCTGACGCTCTCAAGTCACAAGTTCTTCCAACGATTAAGAATTCGCCCAAGTACGGAGGAGTTATGCTTTGGAACAGGTTCTATGACAACGGTTATAGTGCATCCATTAAGGACAGCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

338

Amino Acids

35.82

Weight (kDa)

4.97

Isoelectric Point (pI)

41.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 46 - 286 1.5e-22 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019771)

Species Orthologous Gene IDs
malus_domestica MD01G1212900.v1.1 MD01G1213100.v1.1 MD01G1213300.v1.1 MD07G1282900.v1.1
prunus_persica Prupe.2G305200_v2.0.a1
pyrus_communis pycom01g22400 pycom01g22420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 522
Acc36I ACCTGC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 371
AccBSI CCGCTC 2 cut(s) 869, 876
AccII CGCG 2 cut(s) 463, 479
AclWI GGATC 2 cut(s) 91, 604
AcoI YGGCCR 3 cut(s) 271, 756, 825
AcsI RAATTY 1 cut(s) 931
AcuI CTGAAG 1 cut(s) 378
AcyI GRCGYC 2 cut(s) 372, 630
AfaI GTAC 2 cut(s) 295, 944
AfiI CCNNNNNNNGG 4 cut(s) 290, 472, 878, 944
AgeI ACCGGT 1 cut(s) 644
AjnI CCWGG 3 cut(s) 542, 624, 831
AleI CACNNNNGTG 1 cut(s) 833
AluBI AGCT 5 cut(s) 221, 398, 538, 596, 857
AluI AGCT 5 cut(s) 221, 398, 538, 596, 857
Alw21I GWGCWC 1 cut(s) 540
AlwI GGATC 2 cut(s) 91, 604
AlwNI CAGNNNCTG 2 cut(s) 327, 860
AoxI GGCC 7 cut(s) 151, 271, 744, 756, 813, 825, 864
ApeKI GCWGC 2 cut(s) 593, 854
ApoI RAATTY 1 cut(s) 931
AsiGI ACCGGT 1 cut(s) 644
Asp700I GAANNNNTTC 2 cut(s) 437, 968
AspLEI GCGC 3 cut(s) 374, 465, 523
AspS9I GGNCC 3 cut(s) 151, 744, 813
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 1 cut(s) 614
AxyI CCTNAGG 1 cut(s) 861
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 540
Bbv12I GWGCWC 1 cut(s) 540
BbvI GCAGC 2 cut(s) 605, 866
BceAI ACGGC 2 cut(s) 175, 505
BciT130I CCWGG 3 cut(s) 544, 626, 833
BcnI CCSGG 1 cut(s) 614
BfaI CTAG 2 cut(s) 176, 288
BfoI RGCGCY 1 cut(s) 375
BfuAI ACCTGC 1 cut(s) 335
BglI GCCNNNNNGGC 1 cut(s) 159
BisI GCNGC 7 cut(s) 129, 591, 594, 633, 855, 867, 877
BlsI GCNGC 7 cut(s) 130, 592, 595, 634, 856, 868, 878
Bme1390I CCNGG 4 cut(s) 544, 614, 626, 833
BmgT120I GGNCC 3 cut(s) 151, 744, 813
BmiI GGNNCC 7 cut(s) 152, 172, 373, 737, 753, 806, 822
BmrFI CCNGG 4 cut(s) 544, 614, 626, 833
BmrI ACTGGG 1 cut(s) 757
BmsI GCATC 5 cut(s) 65, 172, 394, 607, 1001
BmuI ACTGGG 1 cut(s) 757
BpuEI CTTGAG 1 cut(s) 887
BpuMI CCSGG 1 cut(s) 614
BsaHI GRCGYC 2 cut(s) 372, 630
BsaJI CCNNGG 3 cut(s) 747, 816, 831
BsaWI WCCGGW 1 cut(s) 644
Bsc4I CCNNNNNNNGG 4 cut(s) 290, 472, 878, 944
Bse118I RCCGGY 3 cut(s) 269, 644, 850
Bse1I ACTGG 3 cut(s) 205, 694, 763
Bse21I CCTNAGG 1 cut(s) 861
BseBI CCWGG 3 cut(s) 544, 626, 833
BseDI CCNNGG 3 cut(s) 747, 816, 831
BseGI GGATG 3 cut(s) 56, 622, 992
BseLI CCNNNNNNNGG 4 cut(s) 290, 472, 878, 944
BseMII CTCAG 3 cut(s) 207, 299, 852
BseNI ACTGG 3 cut(s) 205, 694, 763
BseRI GAGGAG 2 cut(s) 344, 963
BseXI GCAGC 2 cut(s) 605, 866
Bsh1236I CGCG 2 cut(s) 463, 479
BshFI GGCC 7 cut(s) 153, 273, 746, 758, 815, 827, 866
BshNI GGYRCC 1 cut(s) 371
BshTI ACCGGT 1 cut(s) 644
BsiHKAI GWGCWC 1 cut(s) 540
BsiSI CCGG 8 cut(s) 132, 270, 375, 614, 645, 755, 824, 851
BslFI GGGAC 2 cut(s) 73, 488
BslI CCNNNNNNNGG 4 cut(s) 290, 472, 878, 944
BsmFI GGGAC 2 cut(s) 73, 488
BsnI GGCC 7 cut(s) 153, 273, 746, 758, 815, 827, 866
Bsp1286I GDGCHC 1 cut(s) 540
Bsp143I GATC 3 cut(s) 96, 141, 609
BspANI GGCC 7 cut(s) 153, 273, 746, 758, 815, 827, 866
BspCNI CTCAG 3 cut(s) 206, 300, 853
BspFNI CGCG 2 cut(s) 463, 479
BspLI GGNNCC 7 cut(s) 152, 172, 373, 737, 753, 806, 822
BspMI ACCTGC 1 cut(s) 335
BspPI GGATC 2 cut(s) 91, 604
BspT107I GGYRCC 1 cut(s) 371
BsrBI CCGCTC 2 cut(s) 869, 876
BsrFI RCCGGY 3 cut(s) 269, 644, 850
BsrI ACTGG 3 cut(s) 205, 694, 763
BssAI RCCGGY 3 cut(s) 269, 644, 850
BssECI CCNNGG 3 cut(s) 747, 816, 831
BssMI GATC 3 cut(s) 96, 141, 609
BssNI GRCGYC 2 cut(s) 372, 630
Bst2UI CCWGG 3 cut(s) 544, 626, 833
Bst4CI ACNGT 4 cut(s) 580, 707, 776, 983
BstACI GRCGYC 2 cut(s) 372, 630
BstC8I GCNNGC 4 cut(s) 187, 271, 467, 540
BstDEI CTNAG 4 cut(s) 193, 308, 442, 861
BstEII GGTNACC 1 cut(s) 846
BstF5I GGATG 3 cut(s) 56, 622, 992
BstFNI CGCG 2 cut(s) 463, 479
BstH2I RGCGCY 1 cut(s) 375
BstHHI GCGC 3 cut(s) 374, 465, 523
BstKTI GATC 3 cut(s) 99, 144, 612
BstMBI GATC 3 cut(s) 96, 141, 609
BstMWI GCNNNNNNNGC 7 cut(s) 22, 137, 159, 195, 404, 518, 863
BstNI CCWGG 3 cut(s) 544, 626, 833
BstPI GGTNACC 1 cut(s) 846
BstSCI CCNGG 4 cut(s) 542, 612, 624, 831
BstUI CGCG 2 cut(s) 463, 479
BstV1I GCAGC 2 cut(s) 605, 866
Bsu36I CCTNAGG 1 cut(s) 861
BsuRI GGCC 7 cut(s) 153, 273, 746, 758, 815, 827, 866
BtsCI GGATG 3 cut(s) 56, 622, 992
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 1 cut(s) 274
BveI ACCTGC 1 cut(s) 335
Cac8I GCNNGC 4 cut(s) 187, 271, 467, 540
CaiI CAGNNNCTG 2 cut(s) 327, 860
CfoI GCGC 3 cut(s) 374, 465, 523
Cfr10I RCCGGY 3 cut(s) 269, 644, 850
Cfr13I GGNCC 3 cut(s) 151, 744, 813
CseI GACGC 5 cut(s) 485, 638, 725, 794, 905
Csp6I GTAC 2 cut(s) 294, 943
CspAI ACCGGT 1 cut(s) 644
CviAII CATG 3 cut(s) 111, 690, 841
CviQI GTAC 2 cut(s) 294, 943
DdeI CTNAG 4 cut(s) 193, 308, 442, 861
DinI GGCGCC 1 cut(s) 373
DpnI GATC 3 cut(s) 98, 143, 611
DpnII GATC 3 cut(s) 96, 141, 609
EaeI YGGCCR 3 cut(s) 271, 756, 825
EciI GGCGGA 1 cut(s) 894
Ecl136II GAGCTC 1 cut(s) 538
Eco24I GRGCYC 1 cut(s) 540
Eco53kI GAGCTC 1 cut(s) 538
Eco57I CTGAAG 1 cut(s) 378
Eco81I CCTNAGG 1 cut(s) 861
Eco91I GGTNACC 1 cut(s) 846
EcoICRI GAGCTC 1 cut(s) 538
EcoO65I GGTNACC 1 cut(s) 846
EcoRI GAATTC 1 cut(s) 931
EcoRII CCWGG 3 cut(s) 542, 624, 831
EcoT38I GRGCYC 1 cut(s) 540
EgeI GGCGCC 1 cut(s) 373
EheI GGCGCC 1 cut(s) 373
FaeI CATG 3 cut(s) 114, 693, 844
FaqI GGGAC 2 cut(s) 73, 488
FatI CATG 3 cut(s) 110, 689, 840
FauI CCCGC 2 cut(s) 474, 505
Fnu4HI GCNGC 7 cut(s) 129, 591, 594, 633, 855, 867, 877
FokI GGATG 3 cut(s) 43, 629, 979
FriOI GRGCYC 1 cut(s) 540
Fsp4HI GCNGC 7 cut(s) 129, 591, 594, 633, 855, 867, 877
FspBI CTAG 2 cut(s) 176, 288
FspI TGCGCA 1 cut(s) 522
GlaI GCGC 3 cut(s) 373, 464, 522
GluI GCNGC 7 cut(s) 129, 591, 594, 633, 855, 867, 877
HaeII RGCGCY 1 cut(s) 375
HaeIII GGCC 7 cut(s) 153, 273, 746, 758, 815, 827, 866
HapII CCGG 8 cut(s) 132, 270, 375, 614, 645, 755, 824, 851
HgaI GACGC 5 cut(s) 485, 638, 725, 794, 905
HhaI GCGC 3 cut(s) 374, 465, 523
Hin1I GRCGYC 2 cut(s) 372, 630
Hin1II CATG 3 cut(s) 114, 693, 844
Hin6I GCGC 3 cut(s) 372, 463, 521
HinP1I GCGC 3 cut(s) 372, 463, 521
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HpaI GTTAAC 1 cut(s) 214
HpaII CCGG 8 cut(s) 132, 270, 375, 614, 645, 755, 824, 851
HphI GGTGA 1 cut(s) 614
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 4 cut(s) 239, 323, 609, 873
Hpy188III TCNNGA 4 cut(s) 94, 358, 430, 860
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 2 cut(s) 719, 788
HpyAV CCTTC 1 cut(s) 161
HpyCH4III ACNGT 4 cut(s) 580, 707, 776, 983
HpyCH4IV ACGT 1 cut(s) 660
HpyCH4V TGCA 7 cut(s) 114, 189, 300, 407, 701, 770, 992
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 137, 159, 195, 404, 518, 863
HpyF3I CTNAG 4 cut(s) 193, 308, 442, 861
HpySE526I ACGT 1 cut(s) 660
Hsp92I GRCGYC 2 cut(s) 372, 630
Hsp92II CATG 3 cut(s) 114, 693, 844
HspAI GCGC 3 cut(s) 372, 463, 521
KasI GGCGCC 1 cut(s) 371
KroI GCCGGC 1 cut(s) 269
KroNI GCCGGC 1 cut(s) 271
KspAI GTTAAC 1 cut(s) 214
Kzo9I GATC 3 cut(s) 96, 141, 609
LmnI GCTCC 3 cut(s) 601, 862, 874
Lsp1109I GCAGC 2 cut(s) 605, 866
LweI GCATC 5 cut(s) 65, 172, 394, 607, 1001
MaeI CTAG 2 cut(s) 176, 288
MaeII ACGT 1 cut(s) 660
MaeIII GTNAC 4 cut(s) 380, 546, 846, 906
MalI GATC 3 cut(s) 98, 143, 611
MbiI CCGCTC 2 cut(s) 869, 876
MboI GATC 3 cut(s) 96, 141, 609
MboII GAAGA 2 cut(s) 99, 908
MhlI GDGCHC 1 cut(s) 540
MluCI AATT 2 cut(s) 135, 931
Mly113I GGCGCC 1 cut(s) 372
MmeI TCCRAC 3 cut(s) 713, 782, 944
MroNI GCCGGC 1 cut(s) 269
MroXI GAANNNNTTC 2 cut(s) 437, 968
MseI TTAA 4 cut(s) 213, 725, 927, 999
MslI CAYNNNNRTG 1 cut(s) 833
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 8 cut(s) 132, 270, 375, 614, 645, 755, 824, 851
MspR9I CCNGG 4 cut(s) 544, 614, 626, 833
MvaI CCWGG 3 cut(s) 544, 626, 833
MvnI CGCG 2 cut(s) 463, 479
MwoI GCNNNNNNNGC 7 cut(s) 22, 137, 159, 195, 404, 518, 863
NaeI GCCGGC 1 cut(s) 271
NarI GGCGCC 1 cut(s) 372
NciI CCSGG 1 cut(s) 614
NdeII GATC 3 cut(s) 96, 141, 609
NgoMIV GCCGGC 1 cut(s) 269
NlaIII CATG 3 cut(s) 114, 693, 844
NlaIV GGNNCC 7 cut(s) 152, 172, 373, 737, 753, 806, 822
NmuCI GTSAC 2 cut(s) 546, 906
NsbI TGCGCA 1 cut(s) 522
OliI CACNNNNGTG 1 cut(s) 833
PcsI WCGNNNNNNNCGW 2 cut(s) 711, 780
PdiI GCCGGC 1 cut(s) 271
PdmI GAANNNNTTC 2 cut(s) 437, 968
PfoI TCCNGGA 1 cut(s) 612
PinAI ACCGGT 1 cut(s) 644
PkrI GCNGC 7 cut(s) 130, 592, 595, 634, 856, 868, 878
PluTI GGCGCC 1 cut(s) 375
Psp124BI GAGCTC 1 cut(s) 540
Psp6I CCWGG 3 cut(s) 542, 624, 831
PspEI GGTNACC 1 cut(s) 846
PspGI CCWGG 3 cut(s) 542, 624, 831
PspN4I GGNNCC 7 cut(s) 152, 172, 373, 737, 753, 806, 822
PspPI GGNCC 3 cut(s) 151, 744, 813
PstNI CAGNNNCTG 2 cut(s) 327, 860
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 2 cut(s) 295, 944
RsaNI GTAC 2 cut(s) 294, 943
RseI CAYNNNNRTG 1 cut(s) 833
SacI GAGCTC 1 cut(s) 540
SaqAI TTAA 4 cut(s) 213, 725, 927, 999
SatI GCNGC 7 cut(s) 129, 591, 594, 633, 855, 867, 877
Sau3AI GATC 3 cut(s) 96, 141, 609
Sau96I GGNCC 3 cut(s) 151, 744, 813
ScrFI CCNGG 4 cut(s) 544, 614, 626, 833
SduI GDGCHC 1 cut(s) 540
SfaNI GCATC 5 cut(s) 65, 172, 394, 607, 1001
SfoI GGCGCC 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 833
SmlI CTYRAG 1 cut(s) 902
SmoI CTYRAG 1 cut(s) 902
Sse9I AATT 2 cut(s) 135, 931
SspDI GGCGCC 1 cut(s) 371
SspMI CTAG 2 cut(s) 176, 288
SstI GAGCTC 1 cut(s) 540
StyD4I CCNGG 4 cut(s) 542, 612, 624, 831
TaaI ACNGT 4 cut(s) 580, 707, 776, 983
TaiI ACGT 1 cut(s) 663
TaqI TCGA 2 cut(s) 359, 501
TasI AATT 2 cut(s) 135, 931
TauI GCSGC 5 cut(s) 131, 593, 635, 869, 879
Tru1I TTAA 4 cut(s) 213, 725, 927, 999
Tru9I TTAA 4 cut(s) 213, 725, 927, 999
TscAI CASTG 1 cut(s) 281
TseFI GTSAC 2 cut(s) 546, 906
TseI GCWGC 2 cut(s) 593, 854
Tsp45I GTSAC 2 cut(s) 546, 906
TspDTI ATGAA 2 cut(s) 99, 829
TspGWI ACGGA 2 cut(s) 570, 960
TspRI CASTG 1 cut(s) 281
XapI RAATTY 1 cut(s) 931
XmnI GAANNNNTTC 2 cut(s) 437, 968
XspI CTAG 2 cut(s) 176, 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.