pycom01g22420

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0019771
Physical Location & Seq
Reverse (-)
20014434 .. 20015381
948 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g22420.1

Sequence Viewer

Length: 948 bp
ATGTCCCCTCCCAAGCACTTTGAGCAACAACCCAAAAAAACACATACAAAAATGGCATCCAAAACACAAACCCTAGCCCTAACTCTGTCCCTCTTGATCCTCCTTTCTTCATGCAAGTCCTCCCAAGCTGCCGGAATTGCGATCTATTGGGGCCAAAACGGCAACGAAGAAACCCTAGCAGATGCTTGCAACTCAGGCAACTACCAGTTTGTTAACATAGCTTTCCTCACTACTTTCGGAAACAACCAAACCCCTGTCCTAAACCTCGCCGGCCACTGCGACCCCGCTAGTGGTACTTGCACGGGGCTGAGTGCCGACATCAAAACCTGCCAATCACAAAACATAAAAGTCCTCCTCTCGATTGGAGGGGGCTCCGGAAGTTACTCTCTCACTTCAGCTGATGATGCAAGGCAAGTTGCTGATTACATCTGGAACAACTTCTTAGGTGGTCAGTCCGCTTCGCGCCCGCTTGGGGACGCGGTTTTGGACGGCGTTGATTTCGACATTGAGGCGGGTGGTGCGCAGTTCTATGATGAGCTCGCCAGGTCACTCAACGGACACAACGGACAGGCAAAAACGGTCTATTTAGCCGCAGCTCCACAATGTCCGATCCCGGATGCTCACCTAGACGGCGCTATCCAAACCGGTTTATTTGACTACGTTTGGGTTCAGTTCTACAACAACGGCCAATGCCAGTATGCAGACGGTAATGCCGACGCTCTTTTGAACAGTTGGAACCAATGGGCCTCGGTTCCGGCCACCCAGGTGTTCATGGGGTTACCGGCAGCTCCTGAGGCCGCTCCGAGCGGCGGTTTTATTCCTGCTGACGCTCTCAAGTCACAAGTTCTTCCAACTATTAAGAATTCGGCCAAGTATGGAGGAGTTATGCTTTGGAGCAGGCAGTATGACAACGGTTATAGTGCATCCATTAAGGACAGCATAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

316

Amino Acids

33.41

Weight (kDa)

4.97

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 46 - 262 1.3e-21 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019771)

Species Orthologous Gene IDs
malus_domestica MD01G1212900.v1.1 MD01G1213100.v1.1 MD01G1213300.v1.1 MD07G1282900.v1.1
prunus_persica Prupe.2G305200_v2.0.a1
pyrus_communis pycom01g22400 pycom01g22420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 522
Acc36I ACCTGC 1 cut(s) 335
AccBSI CCGCTC 2 cut(s) 800, 807
AccII CGCG 2 cut(s) 463, 479
AccIII TCCGGA 1 cut(s) 374
AciI CCGC 9 cut(s) 285, 456, 467, 479, 512, 591, 798, 807, 810
AclWI GGATC 2 cut(s) 91, 604
AcoI YGGCCR 4 cut(s) 271, 685, 756, 867
AcsI RAATTY 1 cut(s) 862
AcuI CTGAAG 1 cut(s) 378
AfaI GTAC 1 cut(s) 295
AfiI CCNNNNNNNGG 3 cut(s) 290, 472, 809
AgeI ACCGGT 1 cut(s) 644
AgsI TTSAA 1 cut(s) 727
AjnI CCWGG 2 cut(s) 542, 762
AleI CACNNNNGTG 1 cut(s) 764
AluBI AGCT 6 cut(s) 128, 221, 398, 538, 596, 788
AluI AGCT 6 cut(s) 128, 221, 398, 538, 596, 788
Alw21I GWGCWC 1 cut(s) 540
AlwI GGATC 2 cut(s) 91, 604
AlwNI CAGNNNCTG 1 cut(s) 791
Aor13HI TCCGGA 1 cut(s) 374
AoxI GGCC 7 cut(s) 151, 271, 685, 744, 756, 795, 867
ApeKI GCWGC 3 cut(s) 128, 593, 785
ApoI RAATTY 1 cut(s) 862
AsiGI ACCGGT 1 cut(s) 644
Asp700I GAANNNNTTC 1 cut(s) 437
AspLEI GCGC 3 cut(s) 465, 523, 635
AspS9I GGNCC 2 cut(s) 151, 744
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 1 cut(s) 614
AxyI CCTNAGG 1 cut(s) 792
BanII GRGCYC 2 cut(s) 374, 540
Bbv12I GWGCWC 1 cut(s) 540
BbvI GCAGC 3 cut(s) 115, 605, 797
BceAI ACGGC 4 cut(s) 175, 505, 646, 700
BciT130I CCWGG 2 cut(s) 544, 764
BcnI CCSGG 1 cut(s) 614
BfaI CTAG 4 cut(s) 74, 176, 288, 626
BfoI RGCGCY 1 cut(s) 636
BfuAI ACCTGC 1 cut(s) 335
BglI GCCNNNNNGGC 1 cut(s) 159
BisI GCNGC 6 cut(s) 129, 591, 594, 786, 798, 808
BlsI GCNGC 6 cut(s) 130, 592, 595, 787, 799, 809
Bme1390I CCNGG 3 cut(s) 544, 614, 764
BmgT120I GGNCC 2 cut(s) 151, 744
BmiI GGNNCC 4 cut(s) 152, 373, 737, 753
BmrFI CCNGG 3 cut(s) 544, 614, 764
BmsI GCATC 5 cut(s) 65, 172, 394, 607, 932
BpuEI CTTGAG 1 cut(s) 818
BpuMI CCSGG 1 cut(s) 614
BsaJI CCNNGG 2 cut(s) 747, 762
BsaWI WCCGGW 2 cut(s) 374, 644
Bsc4I CCNNNNNNNGG 3 cut(s) 290, 472, 809
Bse118I RCCGGY 3 cut(s) 269, 644, 781
Bse1I ACTGG 2 cut(s) 205, 694
Bse21I CCTNAGG 1 cut(s) 792
BseAI TCCGGA 1 cut(s) 374
BseBI CCWGG 2 cut(s) 544, 764
BseDI CCNNGG 2 cut(s) 747, 762
BseGI GGATG 3 cut(s) 56, 622, 923
BseLI CCNNNNNNNGG 3 cut(s) 290, 472, 809
BseMII CTCAG 3 cut(s) 207, 299, 783
BseNI ACTGG 2 cut(s) 205, 694
BseRI GAGGAG 2 cut(s) 344, 894
BseXI GCAGC 3 cut(s) 115, 605, 797
Bsh1236I CGCG 2 cut(s) 463, 479
BshFI GGCC 7 cut(s) 153, 273, 687, 746, 758, 797, 869
BshTI ACCGGT 1 cut(s) 644
BsiHKAI GWGCWC 1 cut(s) 540
BsiSI CCGG 7 cut(s) 132, 270, 375, 614, 645, 755, 782
BslFI GGGAC 2 cut(s) 73, 488
BslI CCNNNNNNNGG 3 cut(s) 290, 472, 809
BsmFI GGGAC 2 cut(s) 73, 488
BsnI GGCC 7 cut(s) 153, 273, 687, 746, 758, 797, 869
Bsp1286I GDGCHC 2 cut(s) 374, 540
Bsp13I TCCGGA 1 cut(s) 374
Bsp143I GATC 3 cut(s) 96, 141, 609
BspACI CCGC 9 cut(s) 285, 456, 467, 479, 512, 591, 798, 807, 810
BspANI GGCC 7 cut(s) 153, 273, 687, 746, 758, 797, 869
BspCNI CTCAG 3 cut(s) 206, 300, 784
BspEI TCCGGA 1 cut(s) 374
BspFNI CGCG 2 cut(s) 463, 479
BspLI GGNNCC 4 cut(s) 152, 373, 737, 753
BspMI ACCTGC 1 cut(s) 335
BspPI GGATC 2 cut(s) 91, 604
BsrBI CCGCTC 2 cut(s) 800, 807
BsrFI RCCGGY 3 cut(s) 269, 644, 781
BsrI ACTGG 2 cut(s) 205, 694
BssAI RCCGGY 3 cut(s) 269, 644, 781
BssECI CCNNGG 2 cut(s) 747, 762
BssMI GATC 3 cut(s) 96, 141, 609
Bst2UI CCWGG 2 cut(s) 544, 764
Bst4CI ACNGT 4 cut(s) 580, 707, 731, 914
BstC8I GCNNGC 5 cut(s) 187, 271, 467, 540, 899
BstDEI CTNAG 4 cut(s) 193, 308, 442, 792
BstEII GGTNACC 1 cut(s) 777
BstF5I GGATG 3 cut(s) 56, 622, 923
BstFNI CGCG 2 cut(s) 463, 479
BstH2I RGCGCY 1 cut(s) 636
BstHHI GCGC 3 cut(s) 465, 523, 635
BstKTI GATC 3 cut(s) 99, 144, 612
BstMBI GATC 3 cut(s) 96, 141, 609
BstMWI GCNNNNNNNGC 7 cut(s) 22, 137, 159, 195, 404, 518, 794
BstNI CCWGG 2 cut(s) 544, 764
BstPI GGTNACC 1 cut(s) 777
BstSCI CCNGG 3 cut(s) 542, 612, 762
BstUI CGCG 2 cut(s) 463, 479
BstV1I GCAGC 3 cut(s) 115, 605, 797
Bsu36I CCTNAGG 1 cut(s) 792
BsuRI GGCC 7 cut(s) 153, 273, 687, 746, 758, 797, 869
BtsCI GGATG 3 cut(s) 56, 622, 923
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 1 cut(s) 274
BveI ACCTGC 1 cut(s) 335
Cac8I GCNNGC 5 cut(s) 187, 271, 467, 540, 899
CaiI CAGNNNCTG 1 cut(s) 791
CfoI GCGC 3 cut(s) 465, 523, 635
Cfr10I RCCGGY 3 cut(s) 269, 644, 781
Cfr13I GGNCC 2 cut(s) 151, 744
CseI GACGC 3 cut(s) 485, 725, 836
Csp6I GTAC 1 cut(s) 294
CspAI ACCGGT 1 cut(s) 644
CviAII CATG 2 cut(s) 111, 772
CviQI GTAC 1 cut(s) 294
DdeI CTNAG 4 cut(s) 193, 308, 442, 792
DpnI GATC 3 cut(s) 98, 143, 611
DpnII GATC 3 cut(s) 96, 141, 609
EaeI YGGCCR 4 cut(s) 271, 685, 756, 867
Ecl136II GAGCTC 1 cut(s) 538
Eco24I GRGCYC 2 cut(s) 374, 540
Eco53kI GAGCTC 1 cut(s) 538
Eco57I CTGAAG 1 cut(s) 378
Eco81I CCTNAGG 1 cut(s) 792
Eco91I GGTNACC 1 cut(s) 777
EcoICRI GAGCTC 1 cut(s) 538
EcoO65I GGTNACC 1 cut(s) 777
EcoRI GAATTC 1 cut(s) 862
EcoRII CCWGG 2 cut(s) 542, 762
EcoT38I GRGCYC 2 cut(s) 374, 540
FaeI CATG 2 cut(s) 114, 775
FaqI GGGAC 2 cut(s) 73, 488
FatI CATG 2 cut(s) 110, 771
FauI CCCGC 3 cut(s) 292, 474, 505
Fnu4HI GCNGC 6 cut(s) 129, 591, 594, 786, 798, 808
FokI GGATG 3 cut(s) 43, 629, 910
FriOI GRGCYC 2 cut(s) 374, 540
Fsp4HI GCNGC 6 cut(s) 129, 591, 594, 786, 798, 808
FspBI CTAG 4 cut(s) 74, 176, 288, 626
FspI TGCGCA 1 cut(s) 522
GlaI GCGC 3 cut(s) 464, 522, 634
GluI GCNGC 6 cut(s) 129, 591, 594, 786, 798, 808
HaeII RGCGCY 1 cut(s) 636
HaeIII GGCC 7 cut(s) 153, 273, 687, 746, 758, 797, 869
HapII CCGG 7 cut(s) 132, 270, 375, 614, 645, 755, 782
HgaI GACGC 3 cut(s) 485, 725, 836
HhaI GCGC 3 cut(s) 465, 523, 635
Hin1II CATG 2 cut(s) 114, 775
Hin6I GCGC 3 cut(s) 463, 521, 633
HinP1I GCGC 3 cut(s) 463, 521, 633
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HpaI GTTAAC 1 cut(s) 214
HpaII CCGG 7 cut(s) 132, 270, 375, 614, 645, 755, 782
HphI GGTGA 1 cut(s) 614
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 3 cut(s) 239, 609, 804
Hpy188III TCNNGA 5 cut(s) 94, 358, 375, 430, 791
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 1 cut(s) 719
HpyCH4III ACNGT 4 cut(s) 580, 707, 731, 914
HpyCH4IV ACGT 1 cut(s) 660
HpyCH4V TGCA 6 cut(s) 114, 189, 300, 407, 701, 923
HpyF10VI GCNNNNNNNGC 7 cut(s) 22, 137, 159, 195, 404, 518, 794
HpyF3I CTNAG 4 cut(s) 193, 308, 442, 792
HpySE526I ACGT 1 cut(s) 660
Hsp92II CATG 2 cut(s) 114, 775
HspAI GCGC 3 cut(s) 463, 521, 633
Kpn2I TCCGGA 1 cut(s) 374
KroI GCCGGC 1 cut(s) 269
KroNI GCCGGC 1 cut(s) 271
KspAI GTTAAC 1 cut(s) 214
Kzo9I GATC 3 cut(s) 96, 141, 609
LmnI GCTCC 5 cut(s) 377, 601, 793, 805, 894
Lsp1109I GCAGC 3 cut(s) 115, 605, 797
LweI GCATC 5 cut(s) 65, 172, 394, 607, 932
MaeI CTAG 4 cut(s) 74, 176, 288, 626
MaeII ACGT 1 cut(s) 660
MaeIII GTNAC 4 cut(s) 380, 546, 777, 837
MalI GATC 3 cut(s) 98, 143, 611
MbiI CCGCTC 2 cut(s) 800, 807
MboI GATC 3 cut(s) 96, 141, 609
MboII GAAGA 3 cut(s) 99, 179, 839
MhlI GDGCHC 2 cut(s) 374, 540
MluCI AATT 3 cut(s) 135, 862, 943
MmeI TCCRAC 2 cut(s) 713, 875
MroI TCCGGA 1 cut(s) 374
MroNI GCCGGC 1 cut(s) 269
MroXI GAANNNNTTC 1 cut(s) 437
MseI TTAA 4 cut(s) 213, 858, 930, 946
MslI CAYNNNNRTG 1 cut(s) 764
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 7 cut(s) 132, 270, 375, 614, 645, 755, 782
MspR9I CCNGG 3 cut(s) 544, 614, 764
MvaI CCWGG 2 cut(s) 544, 764
MvnI CGCG 2 cut(s) 463, 479
MwoI GCNNNNNNNGC 7 cut(s) 22, 137, 159, 195, 404, 518, 794
NaeI GCCGGC 1 cut(s) 271
NciI CCSGG 1 cut(s) 614
NdeII GATC 3 cut(s) 96, 141, 609
NgoMIV GCCGGC 1 cut(s) 269
NlaIII CATG 2 cut(s) 114, 775
NlaIV GGNNCC 4 cut(s) 152, 373, 737, 753
NmuCI GTSAC 2 cut(s) 546, 837
NsbI TGCGCA 1 cut(s) 522
OliI CACNNNNGTG 1 cut(s) 764
PcsI WCGNNNNNNNCGW 1 cut(s) 711
PdiI GCCGGC 1 cut(s) 271
PdmI GAANNNNTTC 1 cut(s) 437
PfoI TCCNGGA 1 cut(s) 612
PinAI ACCGGT 1 cut(s) 644
PkrI GCNGC 6 cut(s) 130, 592, 595, 787, 799, 809
Psp124BI GAGCTC 1 cut(s) 540
Psp6I CCWGG 2 cut(s) 542, 762
PspEI GGTNACC 1 cut(s) 777
PspGI CCWGG 2 cut(s) 542, 762
PspN4I GGNNCC 4 cut(s) 152, 373, 737, 753
PspPI GGNCC 2 cut(s) 151, 744
PstNI CAGNNNCTG 1 cut(s) 791
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 1 cut(s) 295
RsaNI GTAC 1 cut(s) 294
RseI CAYNNNNRTG 1 cut(s) 764
SacI GAGCTC 1 cut(s) 540
SaqAI TTAA 4 cut(s) 213, 858, 930, 946
SatI GCNGC 6 cut(s) 129, 591, 594, 786, 798, 808
Sau3AI GATC 3 cut(s) 96, 141, 609
Sau96I GGNCC 2 cut(s) 151, 744
ScrFI CCNGG 3 cut(s) 544, 614, 764
SduI GDGCHC 2 cut(s) 374, 540
SfaNI GCATC 5 cut(s) 65, 172, 394, 607, 932
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 1 cut(s) 833
SmoI CTYRAG 1 cut(s) 833
Sse9I AATT 3 cut(s) 135, 862, 943
SsiI CCGC 9 cut(s) 285, 456, 467, 479, 512, 591, 798, 807, 810
SspMI CTAG 4 cut(s) 74, 176, 288, 626
SstI GAGCTC 1 cut(s) 540
StyD4I CCNGG 3 cut(s) 542, 612, 762
TaaI ACNGT 4 cut(s) 580, 707, 731, 914
TaiI ACGT 1 cut(s) 663
TaqI TCGA 2 cut(s) 359, 501
TasI AATT 3 cut(s) 135, 862, 943
TauI GCSGC 3 cut(s) 593, 800, 810
Tru1I TTAA 4 cut(s) 213, 858, 930, 946
Tru9I TTAA 4 cut(s) 213, 858, 930, 946
TscAI CASTG 1 cut(s) 281
TseFI GTSAC 2 cut(s) 546, 837
TseI GCWGC 3 cut(s) 128, 593, 785
Tsp45I GTSAC 2 cut(s) 546, 837
TspDTI ATGAA 2 cut(s) 99, 760
TspGWI ACGGA 2 cut(s) 570, 579
TspRI CASTG 1 cut(s) 281
XapI RAATTY 1 cut(s) 862
XmnI GAANNNNTTC 1 cut(s) 437
XspI CTAG 4 cut(s) 74, 176, 288, 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.