MD01G1213300.v1.1

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0019771
Physical Location & Seq
Reverse (-)
30670365 .. 30671312
948 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1213300.v1.1.491

Sequence Viewer

Length: 948 bp
ATGTCCCCTCCCAAGCACTTTGAGCAACAACCCAAAAAAACACATACAAAAATGGCATCCAAAACACAAACCCTAGCCCTAACTCTGTCCCTCTTGATCCTCATTTCTTCATGCAAGTCCTCCCAAGCCGCCGGAATCGCGACGTATTGGGGCCAAAACGGCAACGAAGGAACCCTAGCAGAAGCTTGCAACTCGGGCAACTACCAGTTTGTTAACATAGCTTTCCTCTCAACTTTTGGAAACAACCAAGCCCCTGTCCTAAACCTCGCCGGCCACTGCAACCCCGCTAGTGGTACTTGCACGGGGCAGAGTGCCGACATCAGAACCTGCCAATCAAAAAACATAAAAGTCCTCCTCTCGATTGGAGGGGCCACCGAAACTTACTCTCTCACTTCAGCTGATGAGGCAAGGCAAGTTGCTGATTACATCTGGAACAACTTCCTAGGTGGTCAGTCAGATTCGCGCCCGCTTGGGGACGCGGTTTTGGACGGCGTCGATTTCGACATTGAGTTGGGTGGTACGCAGTTCTACGACGAGCTCGCCAGGTCACTCAAAGGACACAACGGACAGGGAAAAACGGTCTATTTAGCCGCAGCTCCACAATGCCCGTTCCCGGATACTCACCTAGACGGCGCTATCCAAACCGGTTTATTTGACTACGTTTGGGTTCAGTTCTACAACAACCCCCTAGCCCAATGCCAGTATGCAGACGGTAATGCCGACGCTCTTTTGAGCAGTTGGAACCGATGGGCCTCGGTTTCGGCCACCCAGGTGTTCTTGGGGATACCGGCGTCTACTGATGCCGCGCGCAGCGGATTTATTCCTGCTGATGCTCTCAAGTCACAAGTCCTTCCAACAATTAAGAATTCGGCCAAGTACGGAGGAGTTATGCTTTGGAACAGGTTCTATGACAACGGTTATAGTGCATCCATTAAGGACAGCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

316

Amino Acids

33.78

Weight (kDa)

5.7

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 46 - 266 8e-23 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0019771)

Species Orthologous Gene IDs
malus_domestica MD01G1212900.v1.1 MD01G1213100.v1.1 MD01G1213300.v1.1 MD07G1282900.v1.1
prunus_persica Prupe.2G305200_v2.0.a1
pyrus_communis pycom01g22400 pycom01g22420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 335
AccI GTMKAC 1 cut(s) 794
AccII CGCG 5 cut(s) 140, 463, 479, 806, 808
AciI CCGC 7 cut(s) 129, 285, 467, 479, 591, 804, 813
AclWI GGATC 1 cut(s) 91
AcoI YGGCCR 3 cut(s) 271, 762, 870
AcsI RAATTY 1 cut(s) 865
AcuI CTGAAG 1 cut(s) 378
AcyI GRCGYC 2 cut(s) 492, 791
AfaI GTAC 3 cut(s) 295, 520, 878
AfiI CCNNNNNNNGG 3 cut(s) 290, 472, 613
AgeI ACCGGT 1 cut(s) 644
AjnI CCWGG 2 cut(s) 542, 768
AleI CACNNNNGTG 1 cut(s) 770
AluBI AGCT 5 cut(s) 185, 221, 398, 538, 596
AluI AGCT 5 cut(s) 185, 221, 398, 538, 596
Alw21I GWGCWC 1 cut(s) 540
AlwI GGATC 1 cut(s) 91
AlwNI CAGNNNCTG 1 cut(s) 327
Ama87I CYCGRG 1 cut(s) 193
AoxI GGCC 6 cut(s) 151, 271, 369, 750, 762, 870
ApeKI GCWGC 2 cut(s) 593, 810
ApoI RAATTY 1 cut(s) 865
AsiGI ACCGGT 1 cut(s) 644
Asp700I GAANNNNTTC 2 cut(s) 437, 902
AspA2I CCTAGG 1 cut(s) 442
AspLEI GCGC 4 cut(s) 465, 635, 808, 810
AspS9I GGNCC 3 cut(s) 151, 369, 750
AsuC2I CCSGG 1 cut(s) 614
AsuHPI GGTGA 1 cut(s) 614
AvaI CYCGRG 1 cut(s) 193
AvrII CCTAGG 1 cut(s) 442
BaeI ACNNNNGTAYC 2 cut(s) 776, 809
BanII GRGCYC 1 cut(s) 540
Bbv12I GWGCWC 1 cut(s) 540
BbvI GCAGC 2 cut(s) 605, 822
BccI CCATC 1 cut(s) 741
BceAI ACGGC 3 cut(s) 175, 505, 646
BciT130I CCWGG 2 cut(s) 544, 770
BciVI GTATCC 2 cut(s) 610, 777
BcnI CCSGG 1 cut(s) 614
BfaI CTAG 6 cut(s) 74, 176, 288, 443, 626, 689
BfoI RGCGCY 1 cut(s) 636
BfuAI ACCTGC 1 cut(s) 335
BfuI GTATCC 2 cut(s) 610, 777
BglI GCCNNNNNGGC 1 cut(s) 159
BisI GCNGC 5 cut(s) 129, 591, 594, 804, 811
BlnI CCTAGG 1 cut(s) 442
BlsI GCNGC 5 cut(s) 130, 592, 595, 805, 812
Bme1390I CCNGG 3 cut(s) 544, 614, 770
BmeT110I CYCGRG 1 cut(s) 193
BmgT120I GGNCC 3 cut(s) 151, 369, 750
BmiI GGNNCC 4 cut(s) 152, 172, 370, 743
BmrFI CCNGG 3 cut(s) 544, 614, 770
BmsI GCATC 4 cut(s) 65, 790, 820, 935
BpuEI CTTGAG 1 cut(s) 821
BpuMI CCSGG 1 cut(s) 614
BsaHI GRCGYC 2 cut(s) 492, 791
BsaJI CCNNGG 3 cut(s) 442, 753, 768
BsaWI WCCGGW 1 cut(s) 644
Bsc4I CCNNNNNNNGG 3 cut(s) 290, 472, 613
Bse118I RCCGGY 3 cut(s) 269, 644, 787
Bse1I ACTGG 2 cut(s) 205, 700
BseBI CCWGG 2 cut(s) 544, 770
BseDI CCNNGG 3 cut(s) 442, 753, 768
BseGI GGATG 2 cut(s) 56, 926
BseLI CCNNNNNNNGG 3 cut(s) 290, 472, 613
BseNI ACTGG 2 cut(s) 205, 700
BsePI GCGCGC 1 cut(s) 806
BseRI GAGGAG 2 cut(s) 344, 897
BseXI GCAGC 2 cut(s) 605, 822
Bsh1236I CGCG 5 cut(s) 140, 463, 479, 806, 808
BshFI GGCC 6 cut(s) 153, 273, 371, 752, 764, 872
BshTI ACCGGT 1 cut(s) 644
BsiHKAI GWGCWC 1 cut(s) 540
BsiHKCI CYCGRG 1 cut(s) 193
BsiSI CCGG 5 cut(s) 132, 270, 614, 645, 788
BslFI GGGAC 2 cut(s) 73, 488
BslI CCNNNNNNNGG 3 cut(s) 290, 472, 613
BsmFI GGGAC 2 cut(s) 73, 488
BsnI GGCC 6 cut(s) 153, 273, 371, 752, 764, 872
BsoBI CYCGRG 1 cut(s) 193
Bsp1286I GDGCHC 1 cut(s) 540
Bsp143I GATC 1 cut(s) 96
Bsp68I TCGCGA 1 cut(s) 140
BspACI CCGC 7 cut(s) 129, 285, 467, 479, 591, 804, 813
BspANI GGCC 6 cut(s) 153, 273, 371, 752, 764, 872
BspFNI CGCG 5 cut(s) 140, 463, 479, 806, 808
BspLI GGNNCC 4 cut(s) 152, 172, 370, 743
BspMI ACCTGC 1 cut(s) 335
BspPI GGATC 1 cut(s) 91
BsrFI RCCGGY 3 cut(s) 269, 644, 787
BsrI ACTGG 2 cut(s) 205, 700
BssAI RCCGGY 3 cut(s) 269, 644, 787
BssECI CCNNGG 3 cut(s) 442, 753, 768
BssHII GCGCGC 1 cut(s) 806
BssMI GATC 1 cut(s) 96
BssNI GRCGYC 2 cut(s) 492, 791
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 2 cut(s) 544, 770
Bst4CI ACNGT 3 cut(s) 580, 713, 917
BstACI GRCGYC 2 cut(s) 492, 791
BstC8I GCNNGC 5 cut(s) 187, 271, 467, 540, 808
BstF5I GGATG 2 cut(s) 56, 926
BstFNI CGCG 5 cut(s) 140, 463, 479, 806, 808
BstH2I RGCGCY 1 cut(s) 636
BstHHI GCGC 4 cut(s) 465, 635, 808, 810
BstKTI GATC 1 cut(s) 99
BstMBI GATC 1 cut(s) 96
BstMWI GCNNNNNNNGC 5 cut(s) 22, 137, 159, 195, 404
BstNI CCWGG 2 cut(s) 544, 770
BstSCI CCNGG 3 cut(s) 542, 612, 768
BstUI CGCG 5 cut(s) 140, 463, 479, 806, 808
BstV1I GCAGC 2 cut(s) 605, 822
BsuI GTATCC 2 cut(s) 610, 777
BsuRI GGCC 6 cut(s) 153, 273, 371, 752, 764, 872
BtsCI GGATG 2 cut(s) 56, 926
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 1 cut(s) 274
BtuMI TCGCGA 1 cut(s) 140
BveI ACCTGC 1 cut(s) 335
Cac8I GCNNGC 5 cut(s) 187, 271, 467, 540, 808
CaiI CAGNNNCTG 1 cut(s) 327
CfoI GCGC 4 cut(s) 465, 635, 808, 810
Cfr10I RCCGGY 3 cut(s) 269, 644, 787
Cfr13I GGNCC 3 cut(s) 151, 369, 750
CseI GACGC 4 cut(s) 481, 485, 731, 780
Csp6I GTAC 3 cut(s) 294, 519, 877
CspAI ACCGGT 1 cut(s) 644
CviAII CATG 1 cut(s) 111
CviQI GTAC 3 cut(s) 294, 519, 877
DpnI GATC 1 cut(s) 98
DpnII GATC 1 cut(s) 96
EaeI YGGCCR 3 cut(s) 271, 762, 870
Ecl136II GAGCTC 1 cut(s) 538
Eco130I CCWWGG 1 cut(s) 442
Eco24I GRGCYC 1 cut(s) 540
Eco53kI GAGCTC 1 cut(s) 538
Eco57I CTGAAG 1 cut(s) 378
Eco88I CYCGRG 1 cut(s) 193
EcoICRI GAGCTC 1 cut(s) 538
EcoRI GAATTC 1 cut(s) 865
EcoRII CCWGG 2 cut(s) 542, 768
EcoT14I CCWWGG 1 cut(s) 442
EcoT38I GRGCYC 1 cut(s) 540
ErhI CCWWGG 1 cut(s) 442
FaeI CATG 1 cut(s) 114
FaiI YATR 8 cut(s) 45, 112, 218, 344, 705, 890, 909, 921
FaqI GGGAC 2 cut(s) 73, 488
FatI CATG 1 cut(s) 110
FauI CCCGC 2 cut(s) 292, 474
FblI GTMKAC 1 cut(s) 794
Fnu4HI GCNGC 5 cut(s) 129, 591, 594, 804, 811
FokI GGATG 2 cut(s) 43, 913
FriOI GRGCYC 1 cut(s) 540
Fsp4HI GCNGC 5 cut(s) 129, 591, 594, 804, 811
FspBI CTAG 6 cut(s) 74, 176, 288, 443, 626, 689
GlaI GCGC 4 cut(s) 464, 634, 807, 809
GluI GCNGC 5 cut(s) 129, 591, 594, 804, 811
HaeII RGCGCY 1 cut(s) 636
HaeIII GGCC 6 cut(s) 153, 273, 371, 752, 764, 872
HapII CCGG 5 cut(s) 132, 270, 614, 645, 788
HgaI GACGC 4 cut(s) 481, 485, 731, 780
HhaI GCGC 4 cut(s) 465, 635, 808, 810
Hin1I GRCGYC 2 cut(s) 492, 791
Hin1II CATG 1 cut(s) 114
Hin6I GCGC 4 cut(s) 463, 633, 806, 808
HinP1I GCGC 4 cut(s) 463, 633, 806, 808
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 183
HinfI GANTC 2 cut(s) 135, 458
HpaI GTTAAC 1 cut(s) 214
HpaII CCGG 5 cut(s) 132, 270, 614, 645, 788
HphI GGTGA 1 cut(s) 614
Hpy166II GTNNAC 2 cut(s) 214, 795
Hpy188I TCNGA 2 cut(s) 323, 457
Hpy188III TCNNGA 4 cut(s) 94, 139, 358, 430
Hpy8I GTNNAC 2 cut(s) 214, 795
Hpy99I CGWCG 4 cut(s) 145, 497, 536, 725
HpyAV CCTTC 2 cut(s) 161, 860
HpyCH4III ACNGT 3 cut(s) 580, 713, 917
HpyCH4IV ACGT 2 cut(s) 143, 660
HpyCH4V TGCA 6 cut(s) 114, 189, 279, 300, 707, 926
HpyF10VI GCNNNNNNNGC 5 cut(s) 22, 137, 159, 195, 404
HpySE526I ACGT 2 cut(s) 143, 660
Hsp92I GRCGYC 2 cut(s) 492, 791
Hsp92II CATG 1 cut(s) 114
HspAI GCGC 4 cut(s) 463, 633, 806, 808
KroI GCCGGC 1 cut(s) 269
KroNI GCCGGC 1 cut(s) 271
KspAI GTTAAC 1 cut(s) 214
Kzo9I GATC 1 cut(s) 96
LmnI GCTCC 1 cut(s) 601
Lsp1109I GCAGC 2 cut(s) 605, 822
LweI GCATC 4 cut(s) 65, 790, 820, 935
MaeI CTAG 6 cut(s) 74, 176, 288, 443, 626, 689
MaeII ACGT 2 cut(s) 143, 660
MaeIII GTNAC 2 cut(s) 546, 840
MalI GATC 1 cut(s) 98
MboI GATC 1 cut(s) 96
MboII GAAGA 1 cut(s) 99
MhlI GDGCHC 1 cut(s) 540
MluCI AATT 2 cut(s) 858, 865
MmeI TCCRAC 2 cut(s) 719, 878
MroNI GCCGGC 1 cut(s) 269
MroXI GAANNNNTTC 2 cut(s) 437, 902
MseI TTAA 3 cut(s) 213, 861, 933
MslI CAYNNNNRTG 1 cut(s) 770
MspA1I CMGCKG 2 cut(s) 398, 813
MspI CCGG 5 cut(s) 132, 270, 614, 645, 788
MspR9I CCNGG 3 cut(s) 544, 614, 770
MvaI CCWGG 2 cut(s) 544, 770
MvnI CGCG 5 cut(s) 140, 463, 479, 806, 808
MwoI GCNNNNNNNGC 5 cut(s) 22, 137, 159, 195, 404
NaeI GCCGGC 1 cut(s) 271
NciI CCSGG 1 cut(s) 614
NdeII GATC 1 cut(s) 96
NgoMIV GCCGGC 1 cut(s) 269
NlaIII CATG 1 cut(s) 114
NlaIV GGNNCC 4 cut(s) 152, 172, 370, 743
NmuCI GTSAC 2 cut(s) 546, 840
NruI TCGCGA 1 cut(s) 140
OliI CACNNNNGTG 1 cut(s) 770
PauI GCGCGC 1 cut(s) 806
PcsI WCGNNNNNNNCGW 1 cut(s) 717
PdiI GCCGGC 1 cut(s) 271
PdmI GAANNNNTTC 2 cut(s) 437, 902
PfeI GAWTC 2 cut(s) 135, 458
PflFI GACNNNGTC 1 cut(s) 491
PfoI TCCNGGA 1 cut(s) 612
PinAI ACCGGT 1 cut(s) 644
PkrI GCNGC 5 cut(s) 130, 592, 595, 805, 812
Psp124BI GAGCTC 1 cut(s) 540
Psp6I CCWGG 2 cut(s) 542, 768
PspGI CCWGG 2 cut(s) 542, 768
PspN4I GGNNCC 4 cut(s) 152, 172, 370, 743
PspPI GGNCC 3 cut(s) 151, 369, 750
PstNI CAGNNNCTG 1 cut(s) 327
PsyI GACNNNGTC 1 cut(s) 491
PteI GCGCGC 1 cut(s) 806
PvuII CAGCTG 1 cut(s) 398
RruI TCGCGA 1 cut(s) 140
RsaI GTAC 3 cut(s) 295, 520, 878
RsaNI GTAC 3 cut(s) 294, 519, 877
RseI CAYNNNNRTG 1 cut(s) 770
SacI GAGCTC 1 cut(s) 540
SaqAI TTAA 3 cut(s) 213, 861, 933
SatI GCNGC 5 cut(s) 129, 591, 594, 804, 811
Sau3AI GATC 1 cut(s) 96
Sau96I GGNCC 3 cut(s) 151, 369, 750
ScrFI CCNGG 3 cut(s) 544, 614, 770
SduI GDGCHC 1 cut(s) 540
SfaNI GCATC 4 cut(s) 65, 790, 820, 935
SmiMI CAYNNNNRTG 1 cut(s) 770
SmlI CTYRAG 1 cut(s) 836
SmoI CTYRAG 1 cut(s) 836
Sse9I AATT 2 cut(s) 858, 865
SsiI CCGC 7 cut(s) 129, 285, 467, 479, 591, 804, 813
SspMI CTAG 6 cut(s) 74, 176, 288, 443, 626, 689
SstI GAGCTC 1 cut(s) 540
StyD4I CCNGG 3 cut(s) 542, 612, 768
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 3 cut(s) 580, 713, 917
TaiI ACGT 2 cut(s) 146, 663
TaqI TCGA 3 cut(s) 359, 495, 501
TasI AATT 2 cut(s) 858, 865
TauI GCSGC 3 cut(s) 131, 593, 806
TfiI GAWTC 2 cut(s) 135, 458
Tru1I TTAA 3 cut(s) 213, 861, 933
Tru9I TTAA 3 cut(s) 213, 861, 933
TscAI CASTG 1 cut(s) 281
TseFI GTSAC 2 cut(s) 546, 840
TseI GCWGC 2 cut(s) 593, 810
Tsp45I GTSAC 2 cut(s) 546, 840
TspDTI ATGAA 1 cut(s) 99
TspGWI ACGGA 2 cut(s) 579, 894
TspRI CASTG 1 cut(s) 281
Tth111I GACNNNGTC 1 cut(s) 491
XapI RAATTY 1 cut(s) 865
XmaJI CCTAGG 1 cut(s) 442
XmiI GTMKAC 1 cut(s) 794
XmnI GAANNNNTTC 2 cut(s) 437, 902
XspI CTAG 6 cut(s) 74, 176, 288, 443, 626, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.