MD07G1282900.v1.1

Belongs to the glycosyl hydrolase 18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0019771
Physical Location & Seq
Reverse (-)
34603494 .. 34604436
943 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1282900.v1.1.491

Sequence Viewer

Length: 903 bp
ATGTACCCTCCCAAGCACTTTGAGCAACAACCAAAAAACACACGTACAAAAATGGCAACCAAAACACAAACCCTAGCCCTAACGCTGTCCCTCTTGGTCCTCATTTCTTCATGCAAGTCCTCTGAATCTGCCGGAATCGCCACGTATTGGGGCCAAAACACCAACGAAGGAACCCTAGCAGATGCTTGCAACACAGGCAACTACCAGTTTGTTAACATAGCTTTCCTCACGACTTTCGGCAACAACCAACCCCCTGTCCTGAACCTCGCCGGCCACTGCGACCCCGGCAGTACTTGCAAGGGGCTGAGCTCCGACATCAGAGCCTGCCAATCCAAAAACATAAAAGTCCTCCTCTCGATTGGAGGGGCTGCCGGAAGTTACTCTCTCACTTCAGCTGATGATGCCAGGCAATCAGCTTCGCGCCCGCTTGGAGACGCTGTTTTGGACGGCGTTGATTTCGACATTGAGGCAGGTGGTGGGCAGTTCTATGATGAGCTTGCCAGGTCACTGAACGGACACAAAGGACAGGCAAAAACAGTCTATTTAGCTGCAGCTCCACAATGTCCGTTCCCGGATACTCACCTAGACGGCGCTATCCAAACCGGCTTGTTTGACTATGTTTGGGTCCAGTTCTACAACAACGCCGAATGCCATTATGCAAACGGTAATGCCGACGCTCTTTTGAGCAAGTGGAACCAATGGGCCTCGGTTCCCGCCAGCCAGGTGTTGTTGGGGTTACCGGCTGCTCCTGCGGCTGCTCCGAGCGGCGGATTTGTTCCTGCTGATGCTCTCAAGTCACAGGTTCTTCCAGCTATTAAGAATTCGCCTAAGTATGGAGGAGTTATGCTTTGGAGCAAGCAGTTTGACAACGGTTATAGCGCATCCATTAAGGACAGCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576 GO:0005615 GO:0044421
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18

Protein Analysis

301

Amino Acids

31.76

Weight (kDa)

6.19

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 46 - 248 1.3e-17 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019771)

Species Orthologous Gene IDs
malus_domestica MD01G1212900.v1.1 MD01G1213100.v1.1 MD01G1213300.v1.1 MD07G1282900.v1.1
prunus_persica Prupe.2G305200_v2.0.a1
pyrus_communis pycom01g22400 pycom01g22420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 461
Acc36I ACCTGC 1 cut(s) 461
AccB7I CCANNNNNTGG 1 cut(s) 147
AccBSI CCGCTC 1 cut(s) 765
AccII CGCG 1 cut(s) 421
AciI CCGC 5 cut(s) 425, 714, 752, 765, 768
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 1 cut(s) 820
AcuI CTGAAG 1 cut(s) 375
AfaI GTAC 3 cut(s) 5, 46, 292
AfiI CCNNNNNNNGG 3 cut(s) 147, 767, 833
AflIII ACRYGT 1 cut(s) 41
AjnI CCWGG 3 cut(s) 404, 500, 720
AluBI AGCT 8 cut(s) 221, 309, 395, 416, 496, 548, 554, 812
AluI AGCT 8 cut(s) 221, 309, 395, 416, 496, 548, 554, 812
Alw21I GWGCWC 1 cut(s) 311
Alw26I GTCTC 1 cut(s) 426
AlwNI CAGNNNCTG 1 cut(s) 324
AoxI GGCC 3 cut(s) 151, 271, 702
ApeKI GCWGC 5 cut(s) 368, 548, 551, 743, 755
ApoI RAATTY 1 cut(s) 820
AspLEI GCGC 3 cut(s) 423, 593, 881
AspS9I GGNCC 4 cut(s) 97, 151, 625, 702
AsuC2I CCSGG 2 cut(s) 285, 572
AsuHPI GGTGA 1 cut(s) 572
AvaII GGWCC 2 cut(s) 97, 625
BanII GRGCYC 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 311
BbvI GCAGC 5 cut(s) 355, 535, 563, 730, 742
BceAI ACGGC 2 cut(s) 463, 604
BciT130I CCWGG 3 cut(s) 406, 502, 722
BciVI GTATCC 1 cut(s) 568
BcnI CCSGG 2 cut(s) 285, 572
BcoDI GTCTC 1 cut(s) 426
BfaI CTAG 3 cut(s) 74, 176, 584
BfmI CTRYAG 1 cut(s) 549
BfoI RGCGCY 1 cut(s) 594
BfuAI ACCTGC 1 cut(s) 461
BfuI GTATCC 1 cut(s) 568
BisI GCNGC 7 cut(s) 369, 549, 552, 744, 753, 756, 766
BlpI GCTNAGC 1 cut(s) 305
BlsI GCNGC 7 cut(s) 370, 550, 553, 745, 754, 757, 767
BmcAI AGTACT 1 cut(s) 292
Bme1390I CCNGG 5 cut(s) 285, 406, 502, 572, 722
Bme18I GGWCC 2 cut(s) 97, 625
BmgT120I GGNCC 4 cut(s) 97, 151, 625, 702
BmiI GGNNCC 5 cut(s) 152, 172, 626, 695, 711
BmrFI CCNGG 5 cut(s) 285, 406, 502, 572, 722
BmsI GCATC 4 cut(s) 172, 391, 775, 890
Bpu1102I GCTNAGC 1 cut(s) 305
BpuEI CTTGAG 1 cut(s) 776
BpuMI CCSGG 2 cut(s) 285, 572
BsaAI YACGTR 2 cut(s) 44, 144
BsaJI CCNNGG 2 cut(s) 283, 705
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bsc4I CCNNNNNNNGG 3 cut(s) 147, 767, 833
Bse118I RCCGGY 3 cut(s) 269, 602, 739
Bse1I ACTGG 2 cut(s) 205, 628
BseBI CCWGG 3 cut(s) 406, 502, 722
BseDI CCNNGG 2 cut(s) 283, 705
BseGI GGATG 1 cut(s) 881
BseLI CCNNNNNNNGG 3 cut(s) 147, 767, 833
BseMII CTCAG 1 cut(s) 296
BseNI ACTGG 2 cut(s) 205, 628
BseRI GAGGAG 2 cut(s) 341, 852
BseXI GCAGC 5 cut(s) 355, 535, 563, 730, 742
Bsh1236I CGCG 1 cut(s) 421
BshFI GGCC 3 cut(s) 153, 273, 704
BsiHKAI GWGCWC 1 cut(s) 311
BsiSI CCGG 7 cut(s) 132, 270, 285, 372, 572, 603, 740
BslFI GGGAC 1 cut(s) 73
BslI CCNNNNNNNGG 3 cut(s) 147, 767, 833
BsmAI GTCTC 1 cut(s) 426
BsmBI CGTCTC 1 cut(s) 426
BsmFI GGGAC 1 cut(s) 73
BsmI GAATGC 1 cut(s) 653
BsnI GGCC 3 cut(s) 153, 273, 704
Bsp1286I GDGCHC 1 cut(s) 311
Bsp1720I GCTNAGC 1 cut(s) 305
BspACI CCGC 5 cut(s) 425, 714, 752, 765, 768
BspANI GGCC 3 cut(s) 153, 273, 704
BspCNI CTCAG 1 cut(s) 297
BspFNI CGCG 1 cut(s) 421
BspLI GGNNCC 5 cut(s) 152, 172, 626, 695, 711
BspMAI CTGCAG 1 cut(s) 553
BspMI ACCTGC 1 cut(s) 461
BsrBI CCGCTC 1 cut(s) 765
BsrFI RCCGGY 3 cut(s) 269, 602, 739
BsrI ACTGG 2 cut(s) 205, 628
BssAI RCCGGY 3 cut(s) 269, 602, 739
BssECI CCNNGG 2 cut(s) 283, 705
Bst2UI CCWGG 3 cut(s) 406, 502, 722
Bst4CI ACNGT 3 cut(s) 538, 665, 872
BstAPI GCANNNNNTGC 1 cut(s) 294
BstBAI YACGTR 2 cut(s) 44, 144
BstC8I GCNNGC 7 cut(s) 187, 271, 325, 425, 498, 718, 857
BstDEI CTNAG 2 cut(s) 305, 828
BstEII GGTNACC 1 cut(s) 735
BstF5I GGATG 1 cut(s) 881
BstFNI CGCG 1 cut(s) 421
BstH2I RGCGCY 1 cut(s) 594
BstHHI GCGC 3 cut(s) 423, 593, 881
BstMAI GTCTC 1 cut(s) 426
BstMWI GCNNNNNNNGC 8 cut(s) 22, 137, 195, 285, 294, 401, 749, 752
BstNI CCWGG 3 cut(s) 406, 502, 722
BstPI GGTNACC 1 cut(s) 735
BstSCI CCNGG 5 cut(s) 283, 404, 500, 570, 720
BstSFI CTRYAG 1 cut(s) 549
BstUI CGCG 1 cut(s) 421
BstV1I GCAGC 5 cut(s) 355, 535, 563, 730, 742
BsuI GTATCC 1 cut(s) 568
BsuRI GGCC 3 cut(s) 153, 273, 704
BtsCI GGATG 1 cut(s) 881
BtsI GCAGTG 1 cut(s) 274
BtsIMutI CAGTG 2 cut(s) 274, 506
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 7 cut(s) 187, 271, 325, 425, 498, 718, 857
CaiI CAGNNNCTG 1 cut(s) 324
CfoI GCGC 3 cut(s) 423, 593, 881
Cfr10I RCCGGY 3 cut(s) 269, 602, 739
Cfr13I GGNCC 4 cut(s) 97, 151, 625, 702
CseI GACGC 2 cut(s) 443, 683
Csp6I GTAC 3 cut(s) 4, 45, 291
CviAII CATG 1 cut(s) 111
CviQI GTAC 3 cut(s) 4, 45, 291
DdeI CTNAG 2 cut(s) 305, 828
EaeI YGGCCR 1 cut(s) 271
EciI GGCGGA 1 cut(s) 783
Ecl136II GAGCTC 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 311
Eco47I GGWCC 2 cut(s) 97, 625
Eco53kI GAGCTC 1 cut(s) 309
Eco57I CTGAAG 1 cut(s) 375
Eco91I GGTNACC 1 cut(s) 735
EcoICRI GAGCTC 1 cut(s) 309
EcoO65I GGTNACC 1 cut(s) 735
EcoRI GAATTC 1 cut(s) 820
EcoRII CCWGG 3 cut(s) 404, 500, 720
EcoT38I GRGCYC 1 cut(s) 311
Esp3I CGTCTC 1 cut(s) 426
FaeI CATG 1 cut(s) 114
FaiI YATR 9 cut(s) 112, 218, 341, 489, 618, 657, 834, 845, 876
FaqI GGGAC 1 cut(s) 73
FatI CATG 1 cut(s) 110
FauI CCCGC 2 cut(s) 432, 721
Fnu4HI GCNGC 7 cut(s) 369, 549, 552, 744, 753, 756, 766
FokI GGATG 1 cut(s) 868
FriOI GRGCYC 1 cut(s) 311
Fsp4HI GCNGC 7 cut(s) 369, 549, 552, 744, 753, 756, 766
FspBI CTAG 3 cut(s) 74, 176, 584
GlaI GCGC 3 cut(s) 422, 592, 880
GluI GCNGC 7 cut(s) 369, 549, 552, 744, 753, 756, 766
HaeII RGCGCY 1 cut(s) 594
HaeIII GGCC 3 cut(s) 153, 273, 704
HapII CCGG 7 cut(s) 132, 270, 285, 372, 572, 603, 740
HgaI GACGC 2 cut(s) 443, 683
HhaI GCGC 3 cut(s) 423, 593, 881
Hin1II CATG 1 cut(s) 114
Hin6I GCGC 3 cut(s) 421, 591, 879
HinP1I GCGC 3 cut(s) 421, 591, 879
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 2 cut(s) 125, 135
HpaI GTTAAC 1 cut(s) 214
HpaII CCGG 7 cut(s) 132, 270, 285, 372, 572, 603, 740
HphI GGTGA 1 cut(s) 572
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 4 cut(s) 124, 313, 320, 762
Hpy188III TCNNGA 3 cut(s) 229, 259, 355
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 1 cut(s) 677
HpyAV CCTTC 1 cut(s) 161
HpyCH4III ACNGT 3 cut(s) 538, 665, 872
HpyCH4IV ACGT 2 cut(s) 43, 143
HpyCH4V TGCA 5 cut(s) 114, 189, 297, 551, 659
HpyF10VI GCNNNNNNNGC 8 cut(s) 22, 137, 195, 285, 294, 401, 749, 752
HpyF3I CTNAG 2 cut(s) 305, 828
HpySE526I ACGT 2 cut(s) 43, 143
Hsp92II CATG 1 cut(s) 114
HspAI GCGC 3 cut(s) 421, 591, 879
KroI GCCGGC 1 cut(s) 269
KroNI GCCGGC 1 cut(s) 271
KspAI GTTAAC 1 cut(s) 214
LmnI GCTCC 5 cut(s) 314, 559, 751, 763, 852
Lsp1109I GCAGC 5 cut(s) 355, 535, 563, 730, 742
LweI GCATC 4 cut(s) 172, 391, 775, 890
MaeI CTAG 3 cut(s) 74, 176, 584
MaeII ACGT 2 cut(s) 43, 143
MaeIII GTNAC 4 cut(s) 377, 504, 735, 795
MbiI CCGCTC 1 cut(s) 765
MboII GAAGA 2 cut(s) 99, 797
MhlI GDGCHC 1 cut(s) 311
MluCI AATT 1 cut(s) 820
MmeI TCCRAC 1 cut(s) 336
MroNI GCCGGC 1 cut(s) 269
MseI TTAA 3 cut(s) 213, 816, 888
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 7 cut(s) 132, 270, 285, 372, 572, 603, 740
MspR9I CCNGG 5 cut(s) 285, 406, 502, 572, 722
Mva1269I GAATGC 1 cut(s) 653
MvaI CCWGG 3 cut(s) 406, 502, 722
MvnI CGCG 1 cut(s) 421
MwoI GCNNNNNNNGC 8 cut(s) 22, 137, 195, 285, 294, 401, 749, 752
NaeI GCCGGC 1 cut(s) 271
NciI CCSGG 2 cut(s) 285, 572
NgoMIV GCCGGC 1 cut(s) 269
NlaIII CATG 1 cut(s) 114
NlaIV GGNNCC 5 cut(s) 152, 172, 626, 695, 711
NmuCI GTSAC 2 cut(s) 504, 795
PaqCI CACCTGC 1 cut(s) 461
PcsI WCGNNNNNNNCGW 1 cut(s) 669
PctI GAATGC 1 cut(s) 653
PdiI GCCGGC 1 cut(s) 271
PfeI GAWTC 2 cut(s) 125, 135
PflMI CCANNNNNTGG 1 cut(s) 147
PfoI TCCNGGA 1 cut(s) 570
PkrI GCNGC 7 cut(s) 370, 550, 553, 745, 754, 757, 767
Ppu21I YACGTR 2 cut(s) 44, 144
Psp124BI GAGCTC 1 cut(s) 311
Psp6I CCWGG 3 cut(s) 404, 500, 720
PspEI GGTNACC 1 cut(s) 735
PspGI CCWGG 3 cut(s) 404, 500, 720
PspN4I GGNNCC 5 cut(s) 152, 172, 626, 695, 711
PspPI GGNCC 4 cut(s) 97, 151, 625, 702
PstI CTGCAG 1 cut(s) 553
PstNI CAGNNNCTG 1 cut(s) 324
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 3 cut(s) 5, 46, 292
RsaNI GTAC 3 cut(s) 4, 45, 291
SacI GAGCTC 1 cut(s) 311
SaqAI TTAA 3 cut(s) 213, 816, 888
SatI GCNGC 7 cut(s) 369, 549, 552, 744, 753, 756, 766
Sau96I GGNCC 4 cut(s) 97, 151, 625, 702
ScaI AGTACT 1 cut(s) 292
ScrFI CCNGG 5 cut(s) 285, 406, 502, 572, 722
SduI GDGCHC 1 cut(s) 311
SfaNI GCATC 4 cut(s) 172, 391, 775, 890
SfcI CTRYAG 1 cut(s) 549
SinI GGWCC 2 cut(s) 97, 625
SmlI CTYRAG 1 cut(s) 791
SmoI CTYRAG 1 cut(s) 791
Sse9I AATT 1 cut(s) 820
SsiI CCGC 5 cut(s) 425, 714, 752, 765, 768
SspMI CTAG 3 cut(s) 74, 176, 584
SstI GAGCTC 1 cut(s) 311
StyD4I CCNGG 5 cut(s) 283, 404, 500, 570, 720
TaaI ACNGT 3 cut(s) 538, 665, 872
TaiI ACGT 2 cut(s) 46, 146
TaqI TCGA 2 cut(s) 356, 459
TasI AATT 1 cut(s) 820
TatI WGTACW 1 cut(s) 290
TauI GCSGC 2 cut(s) 755, 768
TfiI GAWTC 2 cut(s) 125, 135
Tru1I TTAA 3 cut(s) 213, 816, 888
Tru9I TTAA 3 cut(s) 213, 816, 888
TscAI CASTG 2 cut(s) 281, 513
TseFI GTSAC 2 cut(s) 504, 795
TseI GCWGC 5 cut(s) 368, 548, 551, 743, 755
Tsp45I GTSAC 2 cut(s) 504, 795
TspDTI ATGAA 1 cut(s) 99
TspGWI ACGGA 2 cut(s) 528, 555
TspRI CASTG 2 cut(s) 281, 513
Van91I CCANNNNNTGG 1 cut(s) 147
VpaK11BI GGWCC 2 cut(s) 97, 625
XapI RAATTY 1 cut(s) 820
XspI CTAG 3 cut(s) 74, 176, 584
ZrmI AGTACT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.