MD01G1219600.v1.1

May be involved in fusion of retrograde transport vesicles derived from an endocytic compartment with the Golgi complex

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013960
Physical Location & Seq
Reverse (-)
31204976 .. 31207457
2482 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1219600.v1.1.491

Sequence Viewer

Length: 489 bp
ATGTGGGGGAAGCTGAACCAATCTGTCCTCGGAGACAATGAAGAGGGAGGAGAGAGCTTACTGGATGAAGAATCTGAGGGCTTGTTTAATCTCTCTGCTACTCAGAGAATGTACGCGTTTGCAGCTTGTTTCTTGGCTGGGATGCTTTGTATGTTCCTGTCGATGATTGTCTTTGCCAAACCCATCAAATTTGCCGTTTTGTTCACCTTCGGCAATTTGTTGGCAGTTGGAAGCACAGCTTTCCTGTTTGGCCCTGCACGACAATTGAGAATGATGTTTGATACTGTTCGAGTGTTTGCAACAGCTATTTACCTTGGATGTGTTGTTCTAGCACTCATTTGCGCTCTCTTGATCCGTAGCAAGATTTTAACAATAATTGCAATCATATGTGAGATTTGCGCCCTTATTTGGTACAGCCTGAGTTACATTCCTTTTGCTCGAAGAATGGTTTCAGGACTGATGATTCGTTTATGTGACACCGAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0006810 GO:0008150 GO:0016020 GO:0016192 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Got1

Protein Analysis

163

Amino Acids

17.97

Weight (kDa)

6.23

Isoelectric Point (pI)

37.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Got1 PF04178 37 - 144 1.3e-32 Got1/Sft2-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013960)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G24170 AT5G24170
fragaria_vesca FvH4_7g31760
malus_domestica MD01G1219600.v1.1
prunus_persica Prupe.2G311600_v2.0.a1 Prupe.2G311600_v2.0.a1 Prupe.2G311600_v2.0.a1 Prupe.2G311600_v2.0.a1
pyrus_communis pycom01g22940
rosa_chinensis RchiOBHm_Chr1g0380991
rosa_laevigata RLG00000026254
rosa_roxburghii Rroxscaffold_4G00278880
rosa_rugosa Rorug01G0426200
rosa_samantha Rh1BG406000 Rh1CG420000 Rh1DG436500
rosa_wichuraiana Rw1G039210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 116
AclWI GGATC 1 cut(s) 346
AcsI RAATTY 1 cut(s) 188
AfaI GTAC 2 cut(s) 113, 413
AfiI CCNNNNNNNGG 1 cut(s) 408
AflIII ACRYGT 1 cut(s) 114
AloI GAACNNNNNNTCC 2 cut(s) 309, 341
AluBI AGCT 6 cut(s) 13, 57, 125, 239, 305, 484
AluI AGCT 6 cut(s) 13, 57, 125, 239, 305, 484
Alw26I GTCTC 1 cut(s) 27
AlwI GGATC 1 cut(s) 346
AoxI GGCC 1 cut(s) 250
ApeKI GCWGC 1 cut(s) 122
ApoI RAATTY 1 cut(s) 188
ArsI GACNNNNNNTTYG 2 cut(s) 447, 479
Asp700I GAANNNNTTC 1 cut(s) 448
AspLEI GCGC 2 cut(s) 344, 401
AspS9I GGNCC 1 cut(s) 251
AsuHPI GGTGA 1 cut(s) 196
BbvI GCAGC 1 cut(s) 134
BccI CCATC 1 cut(s) 191
BceAI ACGGC 1 cut(s) 179
BcoDI GTCTC 1 cut(s) 27
BfaI CTAG 1 cut(s) 329
BisI GCNGC 1 cut(s) 123
BlsI GCNGC 1 cut(s) 124
BmgT120I GGNCC 1 cut(s) 251
BmsI GCATC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 28, 313
Bsc4I CCNNNNNNNGG 1 cut(s) 408
Bse1I ACTGG 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 28, 313
BseGI GGATG 3 cut(s) 70, 147, 323
BseLI CCNNNNNNNGG 1 cut(s) 408
BseMII CTCAG 3 cut(s) 66, 116, 410
BseNI ACTGG 1 cut(s) 66
BseRI GAGGAG 1 cut(s) 63
BseXI GCAGC 1 cut(s) 134
BseYI CCCAGC 1 cut(s) 137
BsgI GTGCAG 1 cut(s) 240
Bsh1236I CGCG 1 cut(s) 116
BshFI GGCC 1 cut(s) 252
BslI CCNNNNNNNGG 1 cut(s) 408
BsmAI GTCTC 1 cut(s) 27
BsnI GGCC 1 cut(s) 252
Bsp143I GATC 1 cut(s) 351
BspANI GGCC 1 cut(s) 252
BspCNI CTCAG 3 cut(s) 67, 115, 411
BspFNI CGCG 1 cut(s) 116
BspPI GGATC 1 cut(s) 346
BsrI ACTGG 1 cut(s) 66
BssECI CCNNGG 2 cut(s) 28, 313
BssMI GATC 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 313
Bst4CI ACNGT 1 cut(s) 286
Bst6I CTCTTC 1 cut(s) 36
BstDEI CTNAG 3 cut(s) 75, 102, 419
BstF5I GGATG 3 cut(s) 70, 147, 323
BstFNI CGCG 1 cut(s) 116
BstHHI GCGC 2 cut(s) 344, 401
BstKTI GATC 1 cut(s) 354
BstMAI GTCTC 1 cut(s) 27
BstMBI GATC 1 cut(s) 351
BstMWI GCNNNNNNNGC 1 cut(s) 122
BstUI CGCG 1 cut(s) 116
BstV1I GCAGC 1 cut(s) 134
BsuRI GGCC 1 cut(s) 252
BtsCI GGATG 3 cut(s) 70, 147, 323
CfoI GCGC 2 cut(s) 344, 401
Cfr13I GGNCC 1 cut(s) 251
Csp6I GTAC 2 cut(s) 112, 412
CviQI GTAC 2 cut(s) 112, 412
DdeI CTNAG 3 cut(s) 75, 102, 419
DpnI GATC 1 cut(s) 353
DpnII GATC 1 cut(s) 351
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 313
EcoT14I CCWWGG 1 cut(s) 313
ErhI CCWWGG 1 cut(s) 313
FaiI YATR 4 cut(s) 152, 386, 388, 472
FalI AAGNNNNNCTT 2 cut(s) 223, 255
FauNDI CATATG 1 cut(s) 386
Fnu4HI GCNGC 1 cut(s) 123
FokI GGATG 3 cut(s) 77, 154, 330
Fsp4HI GCNGC 1 cut(s) 123
FspBI CTAG 1 cut(s) 329
GlaI GCGC 2 cut(s) 343, 400
GluI GCNGC 1 cut(s) 123
GsaI CCCAGC 1 cut(s) 141
HaeIII GGCC 1 cut(s) 252
HhaI GCGC 2 cut(s) 344, 401
Hin6I GCGC 2 cut(s) 342, 399
HinP1I GCGC 2 cut(s) 342, 399
HinfI GANTC 2 cut(s) 71, 463
HphI GGTGA 1 cut(s) 196
Hpy166II GTNNAC 1 cut(s) 204
Hpy188I TCNGA 3 cut(s) 32, 76, 105
Hpy188III TCNNGA 2 cut(s) 349, 453
Hpy8I GTNNAC 1 cut(s) 204
HpyAV CCTTC 1 cut(s) 217
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 4 cut(s) 122, 257, 299, 380
HpyF10VI GCNNNNNNNGC 1 cut(s) 122
HpyF3I CTNAG 3 cut(s) 75, 102, 419
HspAI GCGC 2 cut(s) 342, 399
Kzo9I GATC 1 cut(s) 351
LpnPI CCDG 7 cut(s) 47, 123, 170, 257, 267, 431, 438
Lsp1109I GCAGC 1 cut(s) 134
LweI GCATC 1 cut(s) 132
MaeI CTAG 1 cut(s) 329
MaeIII GTNAC 2 cut(s) 422, 473
MalI GATC 1 cut(s) 353
MboI GATC 1 cut(s) 351
MboII GAAGA 3 cut(s) 53, 80, 453
MfeI CAATTG 1 cut(s) 263
MluCI AATT 4 cut(s) 188, 214, 263, 375
MluI ACGCGT 1 cut(s) 114
MmeI TCCRAC 1 cut(s) 208
MnlI CCTC 4 cut(s) 37, 38, 41, 70
MroXI GAANNNNTTC 1 cut(s) 448
MseI TTAA 2 cut(s) 87, 368
MunI CAATTG 1 cut(s) 263
MvnI CGCG 1 cut(s) 116
MwoI GCNNNNNNNGC 1 cut(s) 122
NdeI CATATG 1 cut(s) 386
NdeII GATC 1 cut(s) 351
NmuCI GTSAC 1 cut(s) 473
PdmI GAANNNNTTC 1 cut(s) 448
PfeI GAWTC 2 cut(s) 71, 463
PkrI GCNGC 1 cut(s) 124
PspFI CCCAGC 1 cut(s) 137
PspPI GGNCC 1 cut(s) 251
RsaI GTAC 2 cut(s) 113, 413
RsaNI GTAC 2 cut(s) 112, 412
SaqAI TTAA 2 cut(s) 87, 368
SatI GCNGC 1 cut(s) 123
Sau3AI GATC 1 cut(s) 351
Sau96I GGNCC 1 cut(s) 251
SetI ASST 8 cut(s) 15, 59, 127, 209, 241, 307, 315, 486
SfaNI GCATC 1 cut(s) 132
Sse9I AATT 4 cut(s) 188, 214, 263, 375
SspMI CTAG 1 cut(s) 329
StyI CCWWGG 1 cut(s) 313
TaaI ACNGT 1 cut(s) 286
TaqI TCGA 3 cut(s) 161, 289, 439
TasI AATT 4 cut(s) 188, 214, 263, 375
TfiI GAWTC 2 cut(s) 71, 463
Tru1I TTAA 2 cut(s) 87, 368
Tru9I TTAA 2 cut(s) 87, 368
TseFI GTSAC 1 cut(s) 473
TseI GCWGC 1 cut(s) 122
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 2 cut(s) 54, 81
TspGWI ACGGA 1 cut(s) 344
XapI RAATTY 1 cut(s) 188
XmnI GAANNNNTTC 1 cut(s) 448
XspI CTAG 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.