MD01G1223300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012245
Physical Location & Seq
Reverse (-)
31508892 .. 31509751
860 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1223300.v1.1.491

Sequence Viewer

Length: 258 bp
ATGGCTGTTAGAATCAATGGCTTTCTTCTTCTGCAAGTGCTTTTCCTCTCAGCACTTCTCATACTTCCCTTATCTTCAGGCATTGTTAATGAAGGCTTCAAGGACATTGATCCCATTCACTTGCTTCACAAGAATGGCGTCCGAATATATTCGAGGAAGCTTTGGAGGCTCGATGCAGCAGTGCACGACTACGACGAAGCAGGACCCAACCCTAAACACGACCCTAGAAGGAAACCCGGCAGTGGCAGGAACCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

86

Amino Acids

9.55

Weight (kDa)

9.86

Isoelectric Point (pI)

30.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 104
AcuI CTGAAG 1 cut(s) 60
AcyI GRCGYC 1 cut(s) 138
AfiI CCNNNNNNNGG 1 cut(s) 242
AgsI TTSAA 1 cut(s) 100
AluBI AGCT 1 cut(s) 160
AluI AGCT 1 cut(s) 160
Alw21I GWGCWC 1 cut(s) 186
Alw44I GTGCAC 1 cut(s) 182
AlwI GGATC 1 cut(s) 104
ApaLI GTGCAC 1 cut(s) 182
ApeKI GCWGC 1 cut(s) 176
Asp700I GAANNNNTTC 1 cut(s) 148
AspS9I GGNCC 1 cut(s) 203
AsuC2I CCSGG 1 cut(s) 237
AvaII GGWCC 1 cut(s) 203
BaeGI GKGCMC 1 cut(s) 186
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 1 cut(s) 188
BcnI CCSGG 1 cut(s) 237
BfaI CTAG 1 cut(s) 225
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 1 cut(s) 237
Bme18I GGWCC 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 2 cut(s) 205, 251
BmrFI CCNGG 1 cut(s) 237
BmsI GCATC 1 cut(s) 163
BpuMI CCSGG 1 cut(s) 237
BsaHI GRCGYC 1 cut(s) 138
Bsc4I CCNNNNNNNGG 1 cut(s) 242
BseLI CCNNNNNNNGG 1 cut(s) 242
BseMII CTCAG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 186
BseXI GCAGC 1 cut(s) 188
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 1 cut(s) 109
BspCNI CTCAG 1 cut(s) 62
BspLI GGNNCC 2 cut(s) 205, 251
BspPI GGATC 1 cut(s) 104
BssMI GATC 1 cut(s) 109
BssNI GRCGYC 1 cut(s) 138
BstACI GRCGYC 1 cut(s) 138
BstDEI CTNAG 1 cut(s) 49
BstKTI GATC 1 cut(s) 112
BstMBI GATC 1 cut(s) 109
BstMWI GCNNNNNNNGC 1 cut(s) 166
BstSCI CCNGG 1 cut(s) 235
BstSLI GKGCMC 1 cut(s) 186
BstV1I GCAGC 1 cut(s) 188
BtsI GCAGTG 2 cut(s) 186, 247
BtsIMutI CAGTG 2 cut(s) 186, 247
Cfr13I GGNCC 1 cut(s) 203
CseI GACGC 1 cut(s) 127
CviAII CATG 1 cut(s) 255
CviJI RGCY 5 cut(s) 5, 21, 96, 160, 169
CviKI_1 RGCY 5 cut(s) 5, 21, 96, 160, 169
DdeI CTNAG 1 cut(s) 49
DpnI GATC 1 cut(s) 111
DpnII GATC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 203
Eco57I CTGAAG 1 cut(s) 60
EcoO109I RGGNCCY 1 cut(s) 203
FaeI CATG 1 cut(s) 258
FaiI YATR 3 cut(s) 62, 148, 256
FatI CATG 1 cut(s) 254
Fnu4HI GCNGC 1 cut(s) 177
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 1 cut(s) 225
GluI GCNGC 1 cut(s) 177
HapII CCGG 1 cut(s) 237
HgaI GACGC 1 cut(s) 127
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 1 cut(s) 258
HindIII AAGCTT 1 cut(s) 158
HinfI GANTC 1 cut(s) 12
HpaII CCGG 1 cut(s) 237
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 1 cut(s) 143
Hpy8I GTNNAC 1 cut(s) 184
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 2 cut(s) 86, 222
HpyCH4V TGCA 3 cut(s) 34, 176, 184
HpyF10VI GCNNNNNNNGC 1 cut(s) 166
HpyF3I CTNAG 1 cut(s) 49
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 1 cut(s) 258
Kzo9I GATC 1 cut(s) 109
LpnPI CCDG 4 cut(s) 63, 186, 232, 250
Lsp1109I GCAGC 1 cut(s) 188
LweI GCATC 1 cut(s) 163
MaeI CTAG 1 cut(s) 225
MalI GATC 1 cut(s) 111
MboI GATC 1 cut(s) 109
MboII GAAGA 3 cut(s) 17, 20, 66
MhlI GDGCHC 1 cut(s) 186
MnlI CCTC 3 cut(s) 56, 147, 159
MroXI GAANNNNTTC 1 cut(s) 148
MseI TTAA 1 cut(s) 87
MslI CAYNNNNRTG 1 cut(s) 132
MspI CCGG 1 cut(s) 237
MspR9I CCNGG 1 cut(s) 237
MwoI GCNNNNNNNGC 1 cut(s) 166
NciI CCSGG 1 cut(s) 237
NdeII GATC 1 cut(s) 109
NlaIII CATG 1 cut(s) 258
NlaIV GGNNCC 2 cut(s) 205, 251
PcsI WCGNNNNNNNCGW 1 cut(s) 192
PdmI GAANNNNTTC 1 cut(s) 148
PfeI GAWTC 1 cut(s) 12
PkrI GCNGC 1 cut(s) 178
PpuMI RGGWCCY 1 cut(s) 203
Psp5II RGGWCCY 1 cut(s) 203
PspN4I GGNNCC 2 cut(s) 205, 251
PspPI GGNCC 1 cut(s) 203
PspPPI RGGWCCY 1 cut(s) 203
RseI CAYNNNNRTG 1 cut(s) 132
SaqAI TTAA 1 cut(s) 87
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 1 cut(s) 109
Sau96I GGNCC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 237
SduI GDGCHC 1 cut(s) 186
SetI ASST 1 cut(s) 162
SfaNI GCATC 1 cut(s) 163
SinI GGWCC 1 cut(s) 203
SmiMI CAYNNNNRTG 1 cut(s) 132
SspMI CTAG 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 235
TaqI TCGA 2 cut(s) 152, 171
TfiI GAWTC 1 cut(s) 12
Tru1I TTAA 1 cut(s) 87
Tru9I TTAA 1 cut(s) 87
TscAI CASTG 2 cut(s) 186, 247
TseI GCWGC 1 cut(s) 176
TspDTI ATGAA 1 cut(s) 105
TspRI CASTG 2 cut(s) 186, 247
VneI GTGCAC 1 cut(s) 182
VpaK11BI GGWCC 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 148
XspI CTAG 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.