MD01G1227000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016927
Physical Location & Seq
Forward (+)
31694272 .. 31695234
963 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1227000.v1.1.491

Sequence Viewer

Length: 963 bp
ATGTTCTTCATTCCTTCAACGCCATTCCTCGACTCCGCCTTCGACGTTGCCACCTTACTATTCATCTCCGCCCTCATCGTCCTCTCCCTCCTCTCCATCTCCTTCATCTTCAACCTCTGCTTAAAGTCCCGCACAGCCCTCCACCTTCGGCACTTCAACTCCCTCTGGACCGTCCGATTCCTCCTCGTCATATTCATCATCTTCTGGTCCCTCAACGAGCTCCTCCGAATCCCCACCCTCCGCCGCACCTACCTCTACCCTTTCCTCTCCGTCCTCGCCTCCTTCGAGGAGCCCCAGTTTTGCAAAATCCACGTGGCGCTCTCGTTAGGGTTATTCGAGCCTGCCTTCCTCGTCACCCTGCTATTCCTTGTCGATGCCTCCATTGAAAGAAAGACCCCCAATGTCTCCTGGGCCATCGCATTCGTCCTCGCCACGTGTCTCCCCATGCTTCTGGTGCAAGTCCTCTTCCTGTTCTTCCACCCGTTCCACAAGCTCCACCTGCCGCTGCCGGAGATTTTCGTACGGAACTATGTCGTCCAGAAGGTCGAGTACGGCAGCAGCATGGTTTCATGCACATACCCTTTGTTGAACACCATCATGTTCGCCGCGTTCGGTGGCGCGTACTCGATGTGGTTCTTGTTTTCGTGCTGGAGGGTTTTGTCGTTGGTGATCAACAAGGGGCTGAGGTTGAGGATTTACACCCTGGCCGCCACGGTATTGGTGCCACTACCACTGCAGATTATTCTCATGGGGATGACCGTGTTGTGGAAGCCGGACCACCCGGCGTTCACCGCCCTCTCATTCGCTGCGTTTTTGTCGACGCTAATTTGTGCAGCCGTGGGGCAGGGCATTTTGGTCATTAAGCCCATCGCAGACTCGTTAGCTGCCGGAGAAGATCGCTGCCGCTTGATTCGGAAGCGGAAGCCGGCGGAGGGGGTGCAGATAATTGTGTCGGCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

321

Amino Acids

36.14

Weight (kDa)

9.35

Isoelectric Point (pI)

35.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016927)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g32590
malus_domestica MD01G1227000.v1.1 MD07G1297300.v1.1
prunus_persica Prupe.2G316900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0382461
rosa_laevigata RLG00000026178
rosa_multiflora Rmu_sc0000110.1_g000049 Rmu_sc0000110.1_g000050 Rmu_sc0003885.1_g000010
rosa_roxburghii Rroxscaffold_4G00278090
rosa_rugosa Rorug01G0434500
rosa_samantha Rh1CG429600 Rh1DG447700
rosa_wichuraiana Rw1G041190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 507
Acc36I ACCTGC 1 cut(s) 507
AccB1I GGYRCC 1 cut(s) 721
AccI GTMKAC 1 cut(s) 818
AccII CGCG 2 cut(s) 608, 620
AcoI YGGCCR 1 cut(s) 705
AcvI CACGTG 2 cut(s) 313, 435
AfaI GTAC 3 cut(s) 522, 551, 623
AfiI CCNNNNNNNGG 2 cut(s) 765, 932
AflIII ACRYGT 1 cut(s) 434
AgsI TTSAA 5 cut(s) 18, 112, 157, 386, 589
AjnI CCWGG 2 cut(s) 407, 702
AluBI AGCT 3 cut(s) 220, 493, 884
AluI AGCT 3 cut(s) 220, 493, 884
Alw21I GWGCWC 1 cut(s) 222
Alw26I GTCTC 2 cut(s) 409, 443
AoxI GGCC 2 cut(s) 411, 705
ApeKI GCWGC 7 cut(s) 505, 555, 558, 806, 833, 884, 900
ArsI GACNNNNNNTTYG 2 cut(s) 23, 55
AspLEI GCGC 2 cut(s) 319, 620
AspS9I GGNCC 4 cut(s) 168, 207, 411, 775
AsuC2I CCSGG 1 cut(s) 782
AsuHPI GGTGA 3 cut(s) 346, 679, 781
AvaII GGWCC 3 cut(s) 168, 207, 775
BanI GGYRCC 1 cut(s) 721
BanII GRGCYC 2 cut(s) 222, 294
BarI GAAGNNNNNNTAC 2 cut(s) 533, 565
BbrPI CACGTG 2 cut(s) 313, 435
Bbv12I GWGCWC 1 cut(s) 222
BbvCI CCTCAGC 1 cut(s) 683
BbvI GCAGC 7 cut(s) 492, 567, 570, 793, 845, 871, 887
BccI CCATC 4 cut(s) 104, 422, 602, 875
BceAI ACGGC 2 cut(s) 568, 821
BciT130I CCWGG 2 cut(s) 409, 704
BclI TGATCA 1 cut(s) 669
BcnI CCSGG 1 cut(s) 782
BcoDI GTCTC 2 cut(s) 409, 443
BfmI CTRYAG 1 cut(s) 734
BfoI RGCGCY 1 cut(s) 320
BfuAI ACCTGC 1 cut(s) 507
Bme1390I CCNGG 3 cut(s) 409, 704, 782
Bme18I GGWCC 3 cut(s) 168, 207, 775
BmgT120I GGNCC 4 cut(s) 168, 207, 411, 775
BmiI GGNNCC 3 cut(s) 209, 291, 723
BmrFI CCNGG 3 cut(s) 409, 704, 782
BmrI ACTGGG 1 cut(s) 289
BmsI GCATC 1 cut(s) 364
BmuI ACTGGG 1 cut(s) 289
BpmI CTGGAG 1 cut(s) 670
Bpu10I CCTNAGC 1 cut(s) 683
BpuMI CCSGG 1 cut(s) 782
BsaAI YACGTR 2 cut(s) 313, 435
BsaJI CCNNGG 4 cut(s) 408, 702, 711, 837
BsaXI ACNNNNNCTCC 4 cut(s) 143, 173, 281, 311
Bsc4I CCNNNNNNNGG 2 cut(s) 765, 932
Bse118I RCCGGY 1 cut(s) 925
Bse1I ACTGG 1 cut(s) 295
BseBI CCWGG 2 cut(s) 409, 704
BseDI CCNNGG 4 cut(s) 408, 702, 711, 837
BseGI GGATG 1 cut(s) 759
BseLI CCNNNNNNNGG 2 cut(s) 765, 932
BseMII CTCAG 1 cut(s) 674
BseNI ACTGG 1 cut(s) 295
BseRI GAGGAG 4 cut(s) 80, 173, 212, 302
BseXI GCAGC 7 cut(s) 492, 567, 570, 793, 845, 871, 887
BsgI GTGCAG 2 cut(s) 852, 959
Bsh1236I CGCG 2 cut(s) 608, 620
BshFI GGCC 2 cut(s) 413, 707
BshNI GGYRCC 1 cut(s) 721
BsiHKAI GWGCWC 1 cut(s) 222
BsiSI CCGG 5 cut(s) 509, 773, 782, 888, 926
BsiWI CGTACG 1 cut(s) 520
BslFI GGGAC 2 cut(s) 112, 193
BslI CCNNNNNNNGG 2 cut(s) 765, 932
BsmAI GTCTC 2 cut(s) 409, 443
BsmFI GGGAC 2 cut(s) 112, 193
BsmI GAATGC 1 cut(s) 419
BsnI GGCC 2 cut(s) 413, 707
Bsp1286I GDGCHC 2 cut(s) 222, 294
Bsp143I GATC 2 cut(s) 669, 895
BspANI GGCC 2 cut(s) 413, 707
BspCNI CTCAG 1 cut(s) 675
BspFNI CGCG 2 cut(s) 608, 620
BspLI GGNNCC 3 cut(s) 209, 291, 723
BspMAI CTGCAG 1 cut(s) 738
BspMI ACCTGC 1 cut(s) 507
BspT107I GGYRCC 1 cut(s) 721
BsrFI RCCGGY 1 cut(s) 925
BsrI ACTGG 1 cut(s) 295
BssAI RCCGGY 1 cut(s) 925
BssECI CCNNGG 4 cut(s) 408, 702, 711, 837
BssMI GATC 2 cut(s) 669, 895
Bst2UI CCWGG 2 cut(s) 409, 704
Bst4CI ACNGT 3 cut(s) 172, 715, 760
Bst6I CTCTTC 1 cut(s) 470
BstBAI YACGTR 2 cut(s) 313, 435
BstC8I GCNNGC 2 cut(s) 342, 927
BstDEI CTNAG 1 cut(s) 683
BstDSI CCRYGG 2 cut(s) 711, 837
BstF5I GGATG 1 cut(s) 759
BstFNI CGCG 2 cut(s) 608, 620
BstH2I RGCGCY 1 cut(s) 320
BstHHI GCGC 2 cut(s) 319, 620
BstKTI GATC 2 cut(s) 672, 898
BstMAI GTCTC 2 cut(s) 409, 443
BstMBI GATC 2 cut(s) 669, 895
BstMWI GCNNNNNNNGC 3 cut(s) 454, 499, 791
BstNI CCWGG 2 cut(s) 409, 704
BstSCI CCNGG 3 cut(s) 407, 702, 780
BstSFI CTRYAG 1 cut(s) 734
BstUI CGCG 2 cut(s) 608, 620
BstV1I GCAGC 7 cut(s) 492, 567, 570, 793, 845, 871, 887
BstXI CCANNNNNNTGG 2 cut(s) 451, 718
BsuRI GGCC 2 cut(s) 413, 707
BtgI CCRYGG 2 cut(s) 711, 837
BtgZI GCGATG 2 cut(s) 400, 853
BtsCI GGATG 1 cut(s) 759
BtsI GCAGTG 1 cut(s) 731
BtsIMutI CAGTG 1 cut(s) 731
BveI ACCTGC 1 cut(s) 507
Cac8I GCNNGC 2 cut(s) 342, 927
CfoI GCGC 2 cut(s) 319, 620
Cfr10I RCCGGY 1 cut(s) 925
Cfr13I GGNCC 4 cut(s) 168, 207, 411, 775
CseI GACGC 1 cut(s) 829
Csp6I GTAC 3 cut(s) 521, 550, 622
CviAII CATG 5 cut(s) 445, 562, 570, 598, 748
CviQI GTAC 3 cut(s) 521, 550, 622
DdeI CTNAG 1 cut(s) 683
DpnI GATC 2 cut(s) 671, 897
DpnII GATC 2 cut(s) 669, 895
EaeI YGGCCR 1 cut(s) 705
Eam1104I CTCTTC 1 cut(s) 470
EarI CTCTTC 1 cut(s) 470
EciI GGCGGA 4 cut(s) 25, 58, 230, 944
Ecl136II GAGCTC 1 cut(s) 220
Eco24I GRGCYC 2 cut(s) 222, 294
Eco47I GGWCC 3 cut(s) 168, 207, 775
Eco53kI GAGCTC 1 cut(s) 220
Eco72I CACGTG 2 cut(s) 313, 435
EcoICRI GAGCTC 1 cut(s) 220
EcoRII CCWGG 2 cut(s) 407, 702
EcoT38I GRGCYC 2 cut(s) 222, 294
FaeI CATG 5 cut(s) 448, 565, 573, 601, 751
FaiI YATR 8 cut(s) 191, 446, 531, 563, 571, 577, 599, 749
FaqI GGGAC 2 cut(s) 112, 193
FatI CATG 5 cut(s) 444, 561, 569, 597, 747
FauI CCCGC 1 cut(s) 137
FbaI TGATCA 1 cut(s) 669
FblI GTMKAC 1 cut(s) 818
FokI GGATG 1 cut(s) 766
FriOI GRGCYC 2 cut(s) 222, 294
GlaI GCGC 2 cut(s) 318, 619
GsuI CTGGAG 1 cut(s) 670
HaeII RGCGCY 1 cut(s) 320
HaeIII GGCC 2 cut(s) 413, 707
HapII CCGG 5 cut(s) 509, 773, 782, 888, 926
HgaI GACGC 1 cut(s) 829
HhaI GCGC 2 cut(s) 319, 620
Hin1II CATG 5 cut(s) 448, 565, 573, 601, 751
Hin6I GCGC 2 cut(s) 317, 618
HinP1I GCGC 2 cut(s) 317, 618
HincII GTYRAC 1 cut(s) 819
HindII GTYRAC 1 cut(s) 819
HinfI GANTC 5 cut(s) 32, 177, 228, 875, 910
HpaII CCGG 5 cut(s) 509, 773, 782, 888, 926
HphI GGTGA 3 cut(s) 346, 679, 781
Hpy166II GTNNAC 2 cut(s) 789, 819
Hpy188I TCNGA 3 cut(s) 176, 227, 915
Hpy188III TCNNGA 2 cut(s) 166, 538
Hpy8I GTNNAC 2 cut(s) 789, 819
Hpy99I CGWCG 2 cut(s) 47, 823
HpyAV CCTTC 7 cut(s) 24, 49, 112, 155, 292, 355, 535
HpyCH4III ACNGT 3 cut(s) 172, 715, 760
HpyCH4IV ACGT 3 cut(s) 45, 312, 434
HpyCH4V TGCA 6 cut(s) 303, 457, 573, 736, 833, 940
HpyF10VI GCNNNNNNNGC 3 cut(s) 454, 499, 791
HpyF3I CTNAG 1 cut(s) 683
HpySE526I ACGT 3 cut(s) 45, 312, 434
Hsp92II CATG 5 cut(s) 448, 565, 573, 601, 751
HspAI GCGC 2 cut(s) 317, 618
KroI GCCGGC 1 cut(s) 925
KroNI GCCGGC 1 cut(s) 927
Ksp22I TGATCA 1 cut(s) 669
Kzo9I GATC 2 cut(s) 669, 895
LmnI GCTCC 3 cut(s) 225, 289, 498
Lsp1109I GCAGC 7 cut(s) 492, 567, 570, 793, 845, 871, 887
LweI GCATC 1 cut(s) 364
MaeII ACGT 3 cut(s) 45, 312, 434
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 671, 897
MboI GATC 2 cut(s) 669, 895
MboII GAAGA 5 cut(s) 100, 193, 457, 466, 905
MhlI GDGCHC 2 cut(s) 222, 294
MluCI AATT 2 cut(s) 825, 945
MlyI GAGTC 2 cut(s) 26, 869
MroNI GCCGGC 1 cut(s) 925
MseI TTAA 2 cut(s) 122, 861
MslI CAYNNNNRTG 2 cut(s) 596, 752
MspA1I CMGCKG 1 cut(s) 505
MspI CCGG 5 cut(s) 509, 773, 782, 888, 926
MspR9I CCNGG 3 cut(s) 409, 704, 782
Mva1269I GAATGC 1 cut(s) 419
MvaI CCWGG 2 cut(s) 409, 704
MvnI CGCG 2 cut(s) 608, 620
MwoI GCNNNNNNNGC 3 cut(s) 454, 499, 791
NaeI GCCGGC 1 cut(s) 927
NciI CCSGG 1 cut(s) 782
NdeII GATC 2 cut(s) 669, 895
NgoMIV GCCGGC 1 cut(s) 925
NlaIII CATG 5 cut(s) 448, 565, 573, 601, 751
NlaIV GGNNCC 3 cut(s) 209, 291, 723
NmuCI GTSAC 1 cut(s) 352
PaqCI CACCTGC 1 cut(s) 507
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PctI GAATGC 1 cut(s) 419
PdiI GCCGGC 1 cut(s) 927
PfeI GAWTC 3 cut(s) 177, 228, 910
Pfl23II CGTACG 1 cut(s) 520
PleI GAGTC 2 cut(s) 26, 869
PmaCI CACGTG 2 cut(s) 313, 435
PmlI CACGTG 2 cut(s) 313, 435
PpsI GAGTC 2 cut(s) 26, 869
Ppu21I YACGTR 2 cut(s) 313, 435
Psp124BI GAGCTC 1 cut(s) 222
Psp6I CCWGG 2 cut(s) 407, 702
PspCI CACGTG 2 cut(s) 313, 435
PspGI CCWGG 2 cut(s) 407, 702
PspLI CGTACG 1 cut(s) 520
PspN4I GGNNCC 3 cut(s) 209, 291, 723
PspPI GGNCC 4 cut(s) 168, 207, 411, 775
PstI CTGCAG 1 cut(s) 738
RsaI GTAC 3 cut(s) 522, 551, 623
RsaNI GTAC 3 cut(s) 521, 550, 622
RseI CAYNNNNRTG 2 cut(s) 596, 752
SacI GAGCTC 1 cut(s) 222
SalI GTCGAC 1 cut(s) 817
SaqAI TTAA 2 cut(s) 122, 861
Sau3AI GATC 2 cut(s) 669, 895
Sau96I GGNCC 4 cut(s) 168, 207, 411, 775
SchI GAGTC 2 cut(s) 26, 869
ScrFI CCNGG 3 cut(s) 409, 704, 782
SduI GDGCHC 2 cut(s) 222, 294
SfaNI GCATC 1 cut(s) 364
SfcI CTRYAG 1 cut(s) 734
SinI GGWCC 3 cut(s) 168, 207, 775
SmiMI CAYNNNNRTG 2 cut(s) 596, 752
Sse9I AATT 2 cut(s) 825, 945
SstI GAGCTC 1 cut(s) 222
StyD4I CCNGG 3 cut(s) 407, 702, 780
TaaI ACNGT 3 cut(s) 172, 715, 760
TaiI ACGT 3 cut(s) 48, 315, 437
TaqI TCGA 8 cut(s) 30, 42, 285, 336, 372, 546, 626, 818
TasI AATT 2 cut(s) 825, 945
TauI GCSGC 5 cut(s) 246, 505, 608, 710, 906
TfiI GAWTC 3 cut(s) 177, 228, 910
Tru1I TTAA 2 cut(s) 122, 861
Tru9I TTAA 2 cut(s) 122, 861
TscAI CASTG 1 cut(s) 738
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 7 cut(s) 505, 555, 558, 806, 833, 884, 900
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 4 cut(s) 52, 94, 184, 558
TspGWI ACGGA 2 cut(s) 259, 538
TspRI CASTG 1 cut(s) 738
VpaK11BI GGWCC 3 cut(s) 168, 207, 775
XmiI GTMKAC 1 cut(s) 818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.