Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0016530

Physical Location & Seq

Genomic Coordinates Forward (+)

32418326 .. 32421061

Gene Structure 2736 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1237000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 798 bp

ATGAGTTTCCAAGATCTCGAGGCTGGTTCTTCTCCCCAACAGCAACACCAACAACGACGACCTTTCGTTTACTCGAATGCGATTCAGAACCAGCAGCAAAATCGAAGTCAAAATCTTTACGAGCCGTCCCAGGCCCTGTCTGCCGGGATTTTCCAAATCAACACCGCCGTATCCACTTTTTACCGCCTCGTCAATTCCCTCGGCACCCCGAGAGACACCCTTCAACTCCGAGACAAGCTGCATAAGACAAGGGCGCATATTGGGCAGTTGGTGAAAGATACTTCAGAAAAACTCAAGCAAGCTAGTGAAACTGATCACCATGCCCAAGTTACTGTTGTCAAGAAGATTGCGGATGCTAAGCTTGCAAAGGATTTCCAGGCCGTACTTAAAGAATTCCAGAAGGCTCAGAGGCTTGCAGCTGAAAGGGAAACAACATATGCTCCCTTTGTTCCCAAAGATGTTCTTCCATCCAGACAAGAGGTTGTACTTGCAGACAATGAGATTGCATTCAATGAAGCCATTATTGAAGAAAGGGAGCAAGGGATCCAAGAAATTCAACAACAGATCAGCGAGGTTAATGAGATTTTCAAAGATCTAGCTGTCCTGGTCCATGATCAAGGAGCTATAATCGATGATATTGGTGCGAACATTGAGAACTCCCACGCTGCAACCGTGCAAGCAACCTCCCACCTTGTGAAAGCATCTAAGACCCAAAGATCTAATTCATCCCTGACGTGTTTGCTGTTGGTGATATTTGGAGTCATCCTCATCATTGTGATTATAGTTGTCATGGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000149 GO:0003674 GO:0005484 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005798 GO:0006605 GO:0006623 GO:0006810 GO:0006886 GO:0006892 GO:0006896 GO:0006906 GO:0006996 GO:0007034 GO:0008104 GO:0008150 GO:0009987 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016021 GO:0016043 GO:0016050 GO:0016192 GO:0022406 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0031201 GO:0031224 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045184 GO:0045324 GO:0046907 GO:0048193 GO:0048278 GO:0048284 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0061024 GO:0061025 GO:0070727 GO:0071702 GO:0071705 GO:0071840 GO:0072594 GO:0072665 GO:0072666 GO:0090174 GO:0097708 GO:0098796 GO:0140056

KEGG Pathways

Metabolic & Signaling

ko04130 ko04145 map04130 map04145

Pfam Domains

Protein Families

SNARE Syntaxin Syntaxin_2

Protein Analysis

266

Amino Acids

29.58

Weight (kDa)

6.25

Isoelectric Point (pI)

48.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Syntaxin_2	PF14523	43 - 157	2.4e-34	Syntaxin-like protein
SNARE	PF05739	209 - 260	1.6e-14	SNARE domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016530)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16830
fragaria_vesca	FvH4_7g34071
malus_domestica	MD01G1237000.v1.1 MD07G1309600.v1.1
prunus_persica	Prupe.2G327300_v2.0.a1
pyrus_communis	pycom01g24480 pycom07g28160
rosa_chinensis	RchiOBHm_Chr1g0384351
rosa_laevigata	RLG00000026046
rosa_multiflora	Rmu_sc0016737.1_g000006
rosa_roxburghii	Rroxscaffold_4G00276750
rosa_rugosa	Rorug01G0447000
rosa_samantha	Rh1CG443100
rosa_wichuraiana	Rw1G040020

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	203
AciI	CCGC	3 cut(s)	165, 184, 350
AclWI	GGATC	2 cut(s)	538, 551
AcsI	RAATTY	2 cut(s)	392, 552
AcuI	CTGAAG	1 cut(s)	267
AdeI	CACNNNGTG	1 cut(s)	694
AfaI	GTAC	2 cut(s)	384, 486
AflIII	ACRYGT	1 cut(s)	734
AgsI	TTSAA	5 cut(s)	224, 511, 527, 557, 589
AjiI	CACGTC	1 cut(s)	735
AjnI	CCWGG	3 cut(s)	129, 375, 603
AluBI	AGCT	6 cut(s)	238, 302, 361, 419, 599, 623
AluI	AGCT	6 cut(s)	238, 302, 361, 419, 599, 623
Alw26I	GTCTC	2 cut(s)	207, 225
AlwI	GGATC	2 cut(s)	538, 551
AlwNI	CAGNNNCTG	1 cut(s)	136
Ama87I	CYCGRG	2 cut(s)	17, 208
AoxI	GGCC	2 cut(s)	132, 378
ApeKI	GCWGC	4 cut(s)	94, 238, 416, 665
ApoI	RAATTY	2 cut(s)	392, 552
ArsI	GACNNNNNNTTYG	2 cut(s)	91, 123
AspLEI	GCGC	1 cut(s)	256
AspS9I	GGNCC	2 cut(s)	133, 607
AsuC2I	CCSGG	1 cut(s)	145
AsuHPI	GGTGA	3 cut(s)	283, 308, 760
AvaI	CYCGRG	2 cut(s)	17, 208
AvaII	GGWCC	1 cut(s)	607
BaeI	ACNNNNGTAYC	2 cut(s)	153, 186
BamHI	GGATCC	1 cut(s)	543
BanI	GGYRCC	1 cut(s)	203
BbvI	GCAGC	4 cut(s)	106, 225, 428, 652
BccI	CCATC	1 cut(s)	475
BceAI	ACGGC	3 cut(s)	109, 152, 365
BcgI	CGANNNNNNTGC	2 cut(s)	83, 117
BciT130I	CCWGG	3 cut(s)	131, 377, 605
BciVI	GTATCC	1 cut(s)	181
BclI	TGATCA	2 cut(s)	313, 613
BcnI	CCSGG	1 cut(s)	145
BcoDI	GTCTC	2 cut(s)	207, 225
BfaI	CTAG	2 cut(s)	303, 596
BfuI	GTATCC	1 cut(s)	181
BglII	AGATCT	3 cut(s)	13, 592, 716
BisI	GCNGC	4 cut(s)	95, 239, 417, 666
BlpI	GCTNAGC	1 cut(s)	357
BlsI	GCNGC	4 cut(s)	96, 240, 418, 667
Bme1390I	CCNGG	4 cut(s)	131, 145, 377, 605
Bme18I	GGWCC	1 cut(s)	607
BmeT110I	CYCGRG	2 cut(s)	17, 208
BmgBI	CACGTC	1 cut(s)	735
BmgT120I	GGNCC	2 cut(s)	133, 607
BmiI	GGNNCC	2 cut(s)	205, 545
BmrFI	CCNGG	4 cut(s)	131, 145, 377, 605
BmsI	GCATC	2 cut(s)	343, 710
BplI	GAGNNNNNCTC	2 cut(s)	750, 782
Bpu1102I	GCTNAGC	1 cut(s)	357
BpuEI	CTTGAG	1 cut(s)	278
BpuMI	CCSGG	1 cut(s)	145
Bsa29I	ATCGAT	1 cut(s)	630
BsaJI	CCNNGG	2 cut(s)	129, 199
BsaXI	ACNNNNNCTCC	2 cut(s)	424, 454
BseBI	CCWGG	3 cut(s)	131, 377, 605
BseCI	ATCGAT	1 cut(s)	630
BseDI	CCNNGG	2 cut(s)	129, 199
BseGI	GGATG	4 cut(s)	358, 467, 725, 762
BseMII	CTCAG	1 cut(s)	419
BseXI	GCAGC	4 cut(s)	106, 225, 428, 652
BshFI	GGCC	2 cut(s)	134, 380
BshNI	GGYRCC	1 cut(s)	203
BshVI	ATCGAT	1 cut(s)	630
BsiHKCI	CYCGRG	2 cut(s)	17, 208
BsiSI	CCGG	1 cut(s)	144
BslFI	GGGAC	1 cut(s)	112
BsmAI	GTCTC	2 cut(s)	207, 225
BsmFI	GGGAC	1 cut(s)	112
BsmI	GAATGC	2 cut(s)	82, 506
BsnI	GGCC	2 cut(s)	134, 380
BsoBI	CYCGRG	2 cut(s)	17, 208
Bsp143I	GATC	7 cut(s)	13, 313, 543, 564, 592, 613, 716
Bsp1720I	GCTNAGC	1 cut(s)	357
BspACI	CCGC	3 cut(s)	165, 184, 350
BspANI	GGCC	2 cut(s)	134, 380
BspCNI	CTCAG	1 cut(s)	418
BspDI	ATCGAT	1 cut(s)	630
BspLI	GGNNCC	2 cut(s)	205, 545
BspPI	GGATC	2 cut(s)	538, 551
BspT107I	GGYRCC	1 cut(s)	203
BssECI	CCNNGG	2 cut(s)	129, 199
BssMI	GATC	7 cut(s)	13, 313, 543, 564, 592, 613, 716
Bst2UI	CCWGG	3 cut(s)	131, 377, 605
Bst4CI	ACNGT	2 cut(s)	334, 673
BstC8I	GCNNGC	4 cut(s)	300, 363, 414, 678
BstDEI	CTNAG	3 cut(s)	357, 405, 705
BstF5I	GGATG	4 cut(s)	358, 467, 725, 762
BstHHI	GCGC	1 cut(s)	256
BstKTI	GATC	7 cut(s)	16, 316, 546, 567, 595, 616, 719
BstMAI	GTCTC	2 cut(s)	207, 225
BstMBI	GATC	7 cut(s)	13, 313, 543, 564, 592, 613, 716
BstMWI	GCNNNNNNNGC	3 cut(s)	140, 262, 362
BstNI	CCWGG	3 cut(s)	131, 377, 605
BstSCI	CCNGG	4 cut(s)	129, 143, 375, 603
BstV1I	GCAGC	4 cut(s)	106, 225, 428, 652
BstX2I	RGATCY	4 cut(s)	13, 543, 592, 716
BstYI	RGATCY	4 cut(s)	13, 543, 592, 716
Bsu15I	ATCGAT	1 cut(s)	630
BsuI	GTATCC	1 cut(s)	181
BsuRI	GGCC	2 cut(s)	134, 380
BsuTUI	ATCGAT	1 cut(s)	630
BtrI	CACGTC	1 cut(s)	735
BtsCI	GGATG	4 cut(s)	358, 467, 725, 762
Cac8I	GCNNGC	4 cut(s)	300, 363, 414, 678
CaiI	CAGNNNCTG	1 cut(s)	136
CfoI	GCGC	1 cut(s)	256
Cfr13I	GGNCC	2 cut(s)	133, 607
ClaI	ATCGAT	1 cut(s)	630
Csp6I	GTAC	2 cut(s)	383, 485
CviAII	CATG	3 cut(s)	320, 611, 790
CviQI	GTAC	2 cut(s)	383, 485
DdeI	CTNAG	3 cut(s)	357, 405, 705
DpnI	GATC	7 cut(s)	15, 315, 545, 566, 594, 615, 718
DpnII	GATC	7 cut(s)	13, 313, 543, 564, 592, 613, 716
DraIII	CACNNNGTG	1 cut(s)	694
Eco47I	GGWCC	1 cut(s)	607
Eco57I	CTGAAG	1 cut(s)	267
Eco88I	CYCGRG	2 cut(s)	17, 208
EcoO109I	RGGNCCY	1 cut(s)	133
EcoRI	GAATTC	1 cut(s)	392
EcoRII	CCWGG	3 cut(s)	129, 375, 603
FaeI	CATG	3 cut(s)	323, 614, 793
FaiI	YATR	9 cut(s)	243, 258, 321, 436, 438, 612, 626, 782, 791
FalI	AAGNNNNNCTT	2 cut(s)	447, 479
FaqI	GGGAC	1 cut(s)	112
FatI	CATG	3 cut(s)	319, 610, 789
FauNDI	CATATG	1 cut(s)	436
FbaI	TGATCA	2 cut(s)	313, 613
Fnu4HI	GCNGC	4 cut(s)	95, 239, 417, 666
FokI	GGATG	4 cut(s)	365, 454, 712, 749
Fsp4HI	GCNGC	4 cut(s)	95, 239, 417, 666
FspBI	CTAG	2 cut(s)	303, 596
GlaI	GCGC	1 cut(s)	255
GluI	GCNGC	4 cut(s)	95, 239, 417, 666
HaeIII	GGCC	2 cut(s)	134, 380
HapII	CCGG	1 cut(s)	144
HhaI	GCGC	1 cut(s)	256
Hin1II	CATG	3 cut(s)	323, 614, 793
Hin6I	GCGC	1 cut(s)	254
HinP1I	GCGC	1 cut(s)	254
HindIII	AAGCTT	1 cut(s)	359
HinfI	GANTC	2 cut(s)	82, 759
HpaII	CCGG	1 cut(s)	144
HphI	GGTGA	3 cut(s)	283, 308, 760
Hpy166II	GTNNAC	1 cut(s)	70
Hpy188I	TCNGA	4 cut(s)	87, 230, 286, 408
Hpy188III	TCNNGA	4 cut(s)	17, 340, 397, 471
Hpy8I	GTNNAC	1 cut(s)	70
Hpy99I	CGWCG	1 cut(s)	60
HpyAV	CCTTC	2 cut(s)	230, 394
HpyCH4III	ACNGT	2 cut(s)	334, 673
HpyCH4IV	ACGT	1 cut(s)	734
HpyCH4V	TGCA	7 cut(s)	241, 365, 416, 491, 506, 668, 676
HpyF10VI	GCNNNNNNNGC	3 cut(s)	140, 262, 362
HpyF3I	CTNAG	3 cut(s)	357, 405, 705
HpySE526I	ACGT	1 cut(s)	734
Hsp92II	CATG	3 cut(s)	323, 614, 793
HspAI	GCGC	1 cut(s)	254
Ksp22I	TGATCA	2 cut(s)	313, 613
Kzo9I	GATC	7 cut(s)	13, 313, 543, 564, 592, 613, 716
LmnI	GCTCC	3 cut(s)	445, 535, 620
Lsp1109I	GCAGC	4 cut(s)	106, 225, 428, 652
LweI	GCATC	2 cut(s)	343, 710
MaeI	CTAG	2 cut(s)	303, 596
MaeII	ACGT	1 cut(s)	734
MaeIII	GTNAC	1 cut(s)	328
MalI	GATC	7 cut(s)	15, 315, 545, 566, 594, 615, 718
MboI	GATC	7 cut(s)	13, 313, 543, 564, 592, 613, 716
MboII	GAAGA	4 cut(s)	21, 355, 455, 539
MflI	RGATCY	4 cut(s)	13, 543, 592, 716
MluCI	AATT	4 cut(s)	193, 392, 552, 721
MlyI	GAGTC	1 cut(s)	768
MnlI	CCTC	8 cut(s)	13, 197, 209, 402, 472, 565, 694, 776
MseI	TTAA	2 cut(s)	387, 576
MslI	CAYNNNNRTG	1 cut(s)	773
MspA1I	CMGCKG	1 cut(s)	419
MspI	CCGG	1 cut(s)	144
MspR9I	CCNGG	4 cut(s)	131, 145, 377, 605
Mva1269I	GAATGC	2 cut(s)	82, 506
MvaI	CCWGG	3 cut(s)	131, 377, 605
MwoI	GCNNNNNNNGC	3 cut(s)	140, 262, 362
NciI	CCSGG	1 cut(s)	145
NdeI	CATATG	1 cut(s)	436
NdeII	GATC	7 cut(s)	13, 313, 543, 564, 592, 613, 716
NlaIII	CATG	3 cut(s)	323, 614, 793
NlaIV	GGNNCC	2 cut(s)	205, 545
NmeAIII	GCCGAG	1 cut(s)	180
PaeR7I	CTCGAG	1 cut(s)	17
PcsI	WCGNNNNNNNCGW	1 cut(s)	669
PctI	GAATGC	2 cut(s)	82, 506
PfeI	GAWTC	1 cut(s)	82
PkrI	GCNGC	4 cut(s)	96, 240, 418, 667
PleI	GAGTC	1 cut(s)	767
PpsI	GAGTC	1 cut(s)	767
Psp6I	CCWGG	3 cut(s)	129, 375, 603
PspGI	CCWGG	3 cut(s)	129, 375, 603
PspN4I	GGNNCC	2 cut(s)	205, 545
PspPI	GGNCC	2 cut(s)	133, 607
PstNI	CAGNNNCTG	1 cut(s)	136
PsuI	RGATCY	4 cut(s)	13, 543, 592, 716
PvuII	CAGCTG	1 cut(s)	419
RsaI	GTAC	2 cut(s)	384, 486
RsaNI	GTAC	2 cut(s)	383, 485
RseI	CAYNNNNRTG	1 cut(s)	773
SaqAI	TTAA	2 cut(s)	387, 576
SatI	GCNGC	4 cut(s)	95, 239, 417, 666
Sau3AI	GATC	7 cut(s)	13, 313, 543, 564, 592, 613, 716
Sau96I	GGNCC	2 cut(s)	133, 607
SchI	GAGTC	1 cut(s)	768
ScrFI	CCNGG	4 cut(s)	131, 145, 377, 605
SfaNI	GCATC	2 cut(s)	343, 710
Sfr274I	CTCGAG	1 cut(s)	17
SinI	GGWCC	1 cut(s)	607
SlaI	CTCGAG	1 cut(s)	17
SmiMI	CAYNNNNRTG	1 cut(s)	773
SmlI	CTYRAG	2 cut(s)	17, 293
SmoI	CTYRAG	2 cut(s)	17, 293
Sse9I	AATT	4 cut(s)	193, 392, 552, 721
SsiI	CCGC	3 cut(s)	165, 184, 350
SspMI	CTAG	2 cut(s)	303, 596
StyD4I	CCNGG	4 cut(s)	129, 143, 375, 603
TaaI	ACNGT	2 cut(s)	334, 673
TaiI	ACGT	1 cut(s)	737
TaqI	TCGA	4 cut(s)	18, 74, 103, 630
TasI	AATT	4 cut(s)	193, 392, 552, 721
TatI	WGTACW	1 cut(s)	484
TfiI	GAWTC	1 cut(s)	82
Tru1I	TTAA	2 cut(s)	387, 576
Tru9I	TTAA	2 cut(s)	387, 576
TseI	GCWGC	4 cut(s)	94, 238, 416, 665
TspDTI	ATGAA	2 cut(s)	528, 714
VpaK11BI	GGWCC	1 cut(s)	607
XapI	RAATTY	2 cut(s)	392, 552
XhoI	CTCGAG	1 cut(s)	17
XspI	CTAG	2 cut(s)	303, 596

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.