Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0013559

Physical Location & Seq

Genomic Coordinates Forward (+)

32610575 .. 32614385

Gene Structure 3811 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1240100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1545 bp

ATGATGGCCACAGCTGCTATTATTGGCCTTGCTACGGGGAAGATGCTCTTGAATTCCTCTTCCTCTTATAATTCAGATATCACAGAAAAACTCTTTCATCTCAATGACCACGCATTCTTACATTGCCATTTCTCTTCGACAAAGAATCTTGTCACTGCCAGAAAGTCTAATTCCAACTACACCAGTTCTAGATTTCCGTCTTCCAATCGACCCGACCGCTCCAGTAGGGCTCTCAAAGAACATGTAGATGCTGCCTTTGGTCCTTCCTCAACCGCACAGCAATGGTTTCACAGCTTCAACGACTTGGAGGAGGAAAGCTCTGATCTTGAATATTCAGTGGACGCACTTCTTTTGCTACAGAAGTCTATGCTCGAAAAGCAATGGAATCTTTCTTTCGAGAAAAAGGTATTTATGGATTCAACAATAAAAACAGCAACGAACAAGAGGATACCAGTCACTTGTTCTGGGGTATCTGCTCGCCACAGGAGAATGAGTACTAGGAGGAATGCTTTCAGTGCAAATCGTTGTCTAACGCAACCTAGCACCAATAAGCCGTTGAGGCCAGTCATGAGTCCAGAGCTGCTTCAGAATCGTTTAAAGGGTTATGTGAAGGGTGTTCTTAGTGAGGATTTGCTGACTCACACTGAGGTTGTACGCCTGTCAAAGAAAATTAAAATTGGCCTCTCTGTCGAGGAGCACAAATCAAGACTGAAGGAGCGACTGGGATGTGAGCCCTCTGATAAACAACTTGCAACTTCATTGAGGATTTCCCGTACTGAATTACTGTCAAAGTTGATCGAATGTTCTTTGGCAAGAGAGAAGCTGGCAATGAGCAATGTTCGTCTGGTCATGTCCATTGCTCAAAAATATGATAATATGGGTGCTGAAATGGCTGACCTAATCCAGGGTGGGTTAATCGGACTATTGCGCGGAATTGAGAAATTTGATTCTTCAAAGGGGTTCAAAATTTCTACTTATGTTTACTGGTGGATACGTCAGGGTGTGTCAAGAGCACTCGTTGAAAATTCGAAAACCTTGAGACTGCCGACACATTTGCACGAGAGGTTAGGTTTAATCAGAAATGCAAAAATTAGACTGGAAGAGAAAGGAATAACTCCATCAATTGATAGAATCGCAGAAAGTCTGAATATGTCCAGAAAGAAAGTTAGGAATGCTACAGAGGCAATCGGTAAGGTTTTTTCACTTGATAGGGATGCATTCCCCTCTTTGAATGGACTTCCAGGAGAAACTCATCATAGTTACATTGCAGATAATCGCCTTGAGAATGTCCCGTGGAATGGGGTGGATGCGTTGGCACTCAAGGATGAAGTAAACAAGCTCATAAATATGATGCTCGGGGATCGGGAGAGAGAGATCATAAGGCTTTACTATGGTCTGGATAATGAATGTCTTACATGGGAGGACATTAGCAAACGTATAGGATTGTCCAGAGAAAGAGTTAGGCAAGTTGGACTTGTTGCTCTTGAGAAACTGAAACATGGTGCGAGGAAAAACGAGATGGAGGCCTTGCTAGTCAAGCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000988 GO:0000990 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009889 GO:0009987 GO:0010109 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016987 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051716 GO:0051775 GO:0060255 GO:0065007 GO:0071214 GO:0071461 GO:0071478 GO:0071482 GO:0071704 GO:0080005 GO:0080090 GO:0090304 GO:0097659 GO:0104004 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:2000112 GO:2000142 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

515

Amino Acids

58.19

Weight (kDa)

9.64

Isoelectric Point (pI)

42.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sigma70_r2	PF04542	276 - 343	1.6e-16	Sigma-70 region 2
Sigma70_r3	PF04539	354 - 424	6.1e-12	Sigma-70 region 3
Sigma70_r4	PF04545	452 - 500	2.6e-10	Sigma-70, region 4

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013559)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64860 AT1G64860
fragaria_vesca	FvH4_7g34280
malus_domestica	MD01G1240100.v1.1 MD07G1311500.v1.1
prunus_persica	Prupe.2G329700_v2.0.a1 Prupe.2G329700_v2.0.a1
pyrus_communis	pycom01g24680 pycom07g28370
rosa_chinensis	RchiOBHm_Chr1g0384631
rosa_laevigata	RLG00000026025
rosa_multiflora	Rmu_sc0012136.1_g000027
rosa_roxburghii	Rroxscaffold_4G00276510
rosa_samantha	Rh1AG474800 Rh1BG432900 Rh1CG446100 Rh1DG465500
rosa_wichuraiana	Rw1G039780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	69
AccBSI	CCGCTC	1 cut(s)	219
AccII	CGCG	1 cut(s)	930
AciI	CCGC	3 cut(s)	217, 273, 930
AclWI	GGATC	1 cut(s)	1368
AcoI	YGGCCR	1 cut(s)	6
AcsI	RAATTY	4 cut(s)	52, 941, 966, 1024
AcuI	CTGAAG	2 cut(s)	569, 731
AfaI	GTAC	3 cut(s)	496, 654, 775
AfiI	CCNNNNNNNGG	3 cut(s)	34, 904, 1298
AflIII	ACRYGT	1 cut(s)	241
AgsI	TTSAA	8 cut(s)	52, 298, 329, 420, 954, 964, 1022, 1231
AjnI	CCWGG	2 cut(s)	903, 1240
AluBI	AGCT	6 cut(s)	14, 294, 318, 580, 823, 1339
AluI	AGCT	6 cut(s)	14, 294, 318, 580, 823, 1339
Alw21I	GWGCWC	2 cut(s)	699, 1015
Alw26I	GTCTC	1 cut(s)	1033
AlwI	GGATC	1 cut(s)	1368
Ama87I	CYCGRG	1 cut(s)	1355
AoxI	GGCC	5 cut(s)	6, 25, 560, 679, 1524
ApeKI	GCWGC	3 cut(s)	14, 251, 580
ApoI	RAATTY	4 cut(s)	52, 941, 966, 1024
Asp700I	GAANNNNTTC	1 cut(s)	509
AspLEI	GCGC	1 cut(s)	930
AspS9I	GGNCC	1 cut(s)	260
AsuII	TTCGAA	1 cut(s)	1028
AvaI	CYCGRG	1 cut(s)	1355
AvaII	GGWCC	1 cut(s)	260
BalI	TGGCCA	1 cut(s)	8
BanII	GRGCYC	2 cut(s)	232, 735
BauI	CACGAG	1 cut(s)	1058
BbsI	GAAGAC	1 cut(s)	192
Bbv12I	GWGCWC	2 cut(s)	699, 1015
BbvI	GCAGC	2 cut(s)	238, 567
BccI	CCATC	2 cut(s)	1126, 1513
BceAI	ACGGC	1 cut(s)	538
BcgI	CGANNNNNNTGC	2 cut(s)	1036, 1070
BciT130I	CCWGG	2 cut(s)	905, 1242
BciVI	GTATCC	2 cut(s)	441, 984
BcoDI	GTCTC	1 cut(s)	1033
BfaI	CTAG	4 cut(s)	189, 498, 540, 1532
BfmI	CTRYAG	2 cut(s)	356, 1176
BfuI	GTATCC	2 cut(s)	441, 984
BglI	GCCNNNNNGGC	1 cut(s)	559
BisI	GCNGC	3 cut(s)	15, 252, 581
BlsI	GCNGC	3 cut(s)	16, 253, 582
BmcAI	AGTACT	1 cut(s)	496
Bme1390I	CCNGG	2 cut(s)	905, 1242
Bme18I	GGWCC	1 cut(s)	260
BmeT110I	CYCGRG	1 cut(s)	1355
BmgT120I	GGNCC	1 cut(s)	260
BmrFI	CCNGG	2 cut(s)	905, 1242
BmrI	ACTGGG	1 cut(s)	731
BmsI	GCATC	5 cut(s)	33, 238, 1204, 1297, 1341
BmuI	ACTGGG	1 cut(s)	731
BpiI	GAAGAC	1 cut(s)	192
BplI	GAGNNNNNCTC	2 cut(s)	302, 334
BpmI	CTGGAG	1 cut(s)	205
Bpu14I	TTCGAA	1 cut(s)	1028
BpuEI	CTTGAG	4 cut(s)	1057, 1301, 1304, 1505
BsaJI	CCNNGG	2 cut(s)	904, 1292
BsaXI	ACNNNNNCTCC	2 cut(s)	478, 508
Bsc4I	CCNNNNNNNGG	3 cut(s)	34, 904, 1298
Bse1I	ACTGG	7 cut(s)	183, 222, 452, 563, 726, 989, 1101
Bse3DI	GCAATG	7 cut(s)	121, 287, 386, 834, 841, 855, 1263
BseBI	CCWGG	2 cut(s)	905, 1242
BseDI	CCNNGG	2 cut(s)	904, 1292
BseGI	GGATG	4 cut(s)	731, 1219, 1312, 1330
BseLI	CCNNNNNNNGG	3 cut(s)	34, 904, 1298
BseMI	GCAATG	7 cut(s)	121, 287, 386, 834, 841, 855, 1263
BseMII	CTCAG	1 cut(s)	636
BseNI	ACTGG	7 cut(s)	183, 222, 452, 563, 726, 989, 1101
BseRI	GAGGAG	2 cut(s)	323, 707
BseXI	GCAGC	2 cut(s)	238, 567
Bsh1236I	CGCG	1 cut(s)	930
Bsh1285I	CGRYCG	1 cut(s)	217
BshFI	GGCC	5 cut(s)	8, 27, 562, 681, 1526
BsiEI	CGRYCG	1 cut(s)	217
BsiHKAI	GWGCWC	2 cut(s)	699, 1015
BsiHKCI	CYCGRG	1 cut(s)	1355
BslFI	GGGAC	1 cut(s)	1274
BslI	CCNNNNNNNGG	3 cut(s)	34, 904, 1298
BsmAI	GTCTC	1 cut(s)	1033
BsmFI	GGGAC	1 cut(s)	1274
BsmI	GAATGC	4 cut(s)	113, 511, 1177, 1217
BsnI	GGCC	5 cut(s)	8, 27, 562, 681, 1526
BsoBI	CYCGRG	1 cut(s)	1355
Bsp119I	TTCGAA	1 cut(s)	1028
Bsp1286I	GDGCHC	4 cut(s)	232, 699, 735, 1015
Bsp143I	GATC	4 cut(s)	322, 795, 1360, 1374
BspACI	CCGC	3 cut(s)	217, 273, 930
BspANI	GGCC	5 cut(s)	8, 27, 562, 681, 1526
BspCNI	CTCAG	1 cut(s)	637
BspFNI	CGCG	1 cut(s)	930
BspHI	TCATGA	1 cut(s)	567
BspPI	GGATC	1 cut(s)	1368
BspT104I	TTCGAA	1 cut(s)	1028
BsrBI	CCGCTC	1 cut(s)	219
BsrDI	GCAATG	7 cut(s)	121, 287, 386, 834, 841, 855, 1263
BsrI	ACTGG	7 cut(s)	183, 222, 452, 563, 726, 989, 1101
BssECI	CCNNGG	2 cut(s)	904, 1292
BssMI	GATC	4 cut(s)	322, 795, 1360, 1374
BssSI	CACGAG	1 cut(s)	1058
Bst2BI	CACGAG	1 cut(s)	1058
Bst2UI	CCWGG	2 cut(s)	905, 1242
Bst4CI	ACNGT	1 cut(s)	786
Bst6I	CTCTTC	3 cut(s)	64, 139, 1095
BstBI	TTCGAA	1 cut(s)	1028
BstC8I	GCNNGC	2 cut(s)	478, 825
BstDEI	CTNAG	2 cut(s)	620, 645
BstDSI	CCRYGG	1 cut(s)	1292
BstENI	CCTNNNNNAGG	1 cut(s)	902
BstF5I	GGATG	4 cut(s)	731, 1219, 1312, 1330
BstFNI	CGCG	1 cut(s)	930
BstHHI	GCGC	1 cut(s)	930
BstKTI	GATC	4 cut(s)	325, 798, 1363, 1377
BstMAI	GTCTC	1 cut(s)	1033
BstMBI	GATC	4 cut(s)	322, 795, 1360, 1374
BstMCI	CGRYCG	1 cut(s)	217
BstMWI	GCNNNNNNNGC	7 cut(s)	14, 376, 515, 559, 890, 1181, 1537
BstNI	CCWGG	2 cut(s)	905, 1242
BstNSI	RCATGY	1 cut(s)	245
BstSCI	CCNGG	2 cut(s)	903, 1240
BstSFI	CTRYAG	2 cut(s)	356, 1176
BstUI	CGCG	1 cut(s)	930
BstV1I	GCAGC	2 cut(s)	238, 567
BstV2I	GAAGAC	1 cut(s)	192
BsuI	GTATCC	2 cut(s)	441, 984
BsuRI	GGCC	5 cut(s)	8, 27, 562, 681, 1526
BtgI	CCRYGG	1 cut(s)	1292
BtsCI	GGATG	4 cut(s)	731, 1219, 1312, 1330
BtsI	GCAGTG	1 cut(s)	153
BtsIMutI	CAGTG	4 cut(s)	153, 342, 520, 642
Cac8I	GCNNGC	2 cut(s)	478, 825
CciI	TCATGA	1 cut(s)	567
CfoI	GCGC	1 cut(s)	930
Cfr13I	GGNCC	1 cut(s)	260
CseI	GACGC	1 cut(s)	350
Csp6I	GTAC	3 cut(s)	495, 653, 774
CviAII	CATG	5 cut(s)	242, 568, 850, 1416, 1499
CviQI	GTAC	3 cut(s)	495, 653, 774
DdeI	CTNAG	2 cut(s)	620, 645
DpnI	GATC	4 cut(s)	324, 797, 1362, 1376
DpnII	GATC	4 cut(s)	322, 795, 1360, 1374
DraI	TTTAAA	1 cut(s)	597
EaeI	YGGCCR	1 cut(s)	6
Eam1104I	CTCTTC	3 cut(s)	64, 139, 1095
EarI	CTCTTC	3 cut(s)	64, 139, 1095
Eco147I	AGGCCT	1 cut(s)	1526
Eco24I	GRGCYC	2 cut(s)	232, 735
Eco32I	GATATC	1 cut(s)	79
Eco47I	GGWCC	1 cut(s)	260
Eco57I	CTGAAG	2 cut(s)	569, 731
Eco88I	CYCGRG	1 cut(s)	1355
EcoNI	CCTNNNNNAGG	1 cut(s)	902
EcoRI	GAATTC	1 cut(s)	52
EcoRII	CCWGG	2 cut(s)	903, 1240
EcoRV	GATATC	1 cut(s)	79
EcoT22I	ATGCAT	1 cut(s)	1219
EcoT38I	GRGCYC	2 cut(s)	232, 735
FaeI	CATG	5 cut(s)	245, 571, 853, 1419, 1502
FalI	AAGNNNNNCTT	4 cut(s)	32, 64, 1458, 1490
FaqI	GGGAC	1 cut(s)	1274
FatI	CATG	5 cut(s)	241, 567, 849, 1415, 1498
Fnu4HI	GCNGC	3 cut(s)	15, 252, 581
FokI	GGATG	4 cut(s)	738, 1226, 1319, 1337
FriOI	GRGCYC	2 cut(s)	232, 735
Fsp4HI	GCNGC	3 cut(s)	15, 252, 581
FspBI	CTAG	4 cut(s)	189, 498, 540, 1532
GlaI	GCGC	1 cut(s)	929
GluI	GCNGC	3 cut(s)	15, 252, 581
GsuI	CTGGAG	1 cut(s)	205
HaeIII	GGCC	5 cut(s)	8, 27, 562, 681, 1526
HgaI	GACGC	1 cut(s)	350
HhaI	GCGC	1 cut(s)	930
Hin1II	CATG	5 cut(s)	245, 571, 853, 1419, 1502
Hin6I	GCGC	1 cut(s)	928
HinP1I	GCGC	1 cut(s)	928
HinfI	GANTC	8 cut(s)	145, 385, 416, 571, 589, 637, 947, 1131
Hpy166II	GTNNAC	3 cut(s)	340, 982, 1333
Hpy188I	TCNGA	7 cut(s)	76, 322, 588, 739, 920, 1079, 1146
Hpy8I	GTNNAC	3 cut(s)	340, 982, 1333
HpyAV	CCTTC	3 cut(s)	273, 604, 706
HpyCH4III	ACNGT	1 cut(s)	786
HpyCH4IV	ACGT	2 cut(s)	994, 1435
HpyCH4V	TGCA	6 cut(s)	518, 752, 1057, 1085, 1217, 1268
HpyF10VI	GCNNNNNNNGC	7 cut(s)	14, 376, 515, 559, 890, 1181, 1537
HpyF3I	CTNAG	2 cut(s)	620, 645
HpySE526I	ACGT	2 cut(s)	994, 1435
Hsp92II	CATG	5 cut(s)	245, 571, 853, 1419, 1502
HspAI	GCGC	1 cut(s)	928
Kzo9I	GATC	4 cut(s)	322, 795, 1360, 1374
LmnI	GCTCC	3 cut(s)	224, 694, 715
Lsp1109I	GCAGC	2 cut(s)	238, 567
LweI	GCATC	5 cut(s)	33, 238, 1204, 1297, 1341
MaeI	CTAG	4 cut(s)	189, 498, 540, 1532
MaeII	ACGT	2 cut(s)	994, 1435
MaeIII	GTNAC	3 cut(s)	151, 454, 1259
MalI	GATC	4 cut(s)	324, 797, 1362, 1376
MbiI	CCGCTC	1 cut(s)	219
MboI	GATC	4 cut(s)	322, 795, 1360, 1374
MboII	GAAGA	6 cut(s)	51, 52, 126, 192, 942, 1112
MfeI	CAATTG	1 cut(s)	1122
MhlI	GDGCHC	4 cut(s)	232, 699, 735, 1015
MlsI	TGGCCA	1 cut(s)	8
MluNI	TGGCCA	1 cut(s)	8
MlyI	GAGTC	2 cut(s)	580, 631
MmeI	TCCRAC	2 cut(s)	198, 1450
Mox20I	TGGCCA	1 cut(s)	8
Mph1103I	ATGCAT	1 cut(s)	1219
MroXI	GAANNNNTTC	1 cut(s)	509
MscI	TGGCCA	1 cut(s)	8
MseI	TTAA	5 cut(s)	596, 672, 914, 1073, 1543
MslI	CAYNNNNRTG	4 cut(s)	102, 246, 280, 1346
Msp20I	TGGCCA	1 cut(s)	8
MspA1I	CMGCKG	1 cut(s)	14
MspR9I	CCNGG	2 cut(s)	905, 1242
MunI	CAATTG	1 cut(s)	1122
Mva1269I	GAATGC	4 cut(s)	113, 511, 1177, 1217
MvaI	CCWGG	2 cut(s)	905, 1242
MvnI	CGCG	1 cut(s)	930
MwoI	GCNNNNNNNGC	7 cut(s)	14, 376, 515, 559, 890, 1181, 1537
NdeII	GATC	4 cut(s)	322, 795, 1360, 1374
NlaIII	CATG	5 cut(s)	245, 571, 853, 1419, 1502
NmuCI	GTSAC	2 cut(s)	151, 454
NsiI	ATGCAT	1 cut(s)	1219
NspI	RCATGY	1 cut(s)	245
NspV	TTCGAA	1 cut(s)	1028
PagI	TCATGA	1 cut(s)	567
PceI	AGGCCT	1 cut(s)	1526
PciI	ACATGT	1 cut(s)	241
PctI	GAATGC	4 cut(s)	113, 511, 1177, 1217
PdmI	GAANNNNTTC	1 cut(s)	509
PfeI	GAWTC	6 cut(s)	145, 385, 416, 589, 947, 1131
PfoI	TCCNGGA	1 cut(s)	1240
PkrI	GCNGC	3 cut(s)	16, 253, 582
PleI	GAGTC	2 cut(s)	579, 631
PpsI	GAGTC	2 cut(s)	579, 631
PscI	ACATGT	1 cut(s)	241
PsiI	TTATAA	1 cut(s)	69
Psp6I	CCWGG	2 cut(s)	903, 1240
PspGI	CCWGG	2 cut(s)	903, 1240
PspPI	GGNCC	1 cut(s)	260
PvuII	CAGCTG	1 cut(s)	14
RsaI	GTAC	3 cut(s)	496, 654, 775
RsaNI	GTAC	3 cut(s)	495, 653, 774
RseI	CAYNNNNRTG	4 cut(s)	102, 246, 280, 1346
SaqAI	TTAA	5 cut(s)	596, 672, 914, 1073, 1543
SatI	GCNGC	3 cut(s)	15, 252, 581
Sau3AI	GATC	4 cut(s)	322, 795, 1360, 1374
Sau96I	GGNCC	1 cut(s)	260
ScaI	AGTACT	1 cut(s)	496
SchI	GAGTC	2 cut(s)	580, 631
ScrFI	CCNGG	2 cut(s)	905, 1242
SduI	GDGCHC	4 cut(s)	232, 699, 735, 1015
SfaNI	GCATC	5 cut(s)	33, 238, 1204, 1297, 1341
SfcI	CTRYAG	2 cut(s)	356, 1176
SfuI	TTCGAA	1 cut(s)	1028
SinI	GGWCC	1 cut(s)	260
SmiMI	CAYNNNNRTG	4 cut(s)	102, 246, 280, 1346
SmlI	CTYRAG	4 cut(s)	1036, 1280, 1319, 1484
SmoI	CTYRAG	4 cut(s)	1036, 1280, 1319, 1484
SseBI	AGGCCT	1 cut(s)	1526
SsiI	CCGC	3 cut(s)	217, 273, 930
SspI	AATATT	1 cut(s)	332
SspMI	CTAG	4 cut(s)	189, 498, 540, 1532
StuI	AGGCCT	1 cut(s)	1526
StyD4I	CCNGG	2 cut(s)	903, 1240
TaaI	ACNGT	1 cut(s)	786
TaiI	ACGT	2 cut(s)	997, 1438
TaqI	TCGA	7 cut(s)	137, 208, 372, 396, 690, 798, 1028
TatI	WGTACW	1 cut(s)	494
TfiI	GAWTC	6 cut(s)	145, 385, 416, 589, 947, 1131
Tru1I	TTAA	5 cut(s)	596, 672, 914, 1073, 1543
Tru9I	TTAA	5 cut(s)	596, 672, 914, 1073, 1543
TscAI	CASTG	4 cut(s)	160, 342, 520, 649
TseFI	GTSAC	2 cut(s)	151, 454
TseI	GCWGC	3 cut(s)	14, 251, 580
Tsp45I	GTSAC	2 cut(s)	151, 454
TspDTI	ATGAA	4 cut(s)	86, 747, 1341, 1419
TspGWI	ACGGA	1 cut(s)	186
TspRI	CASTG	4 cut(s)	160, 342, 520, 649
VpaK11BI	GGWCC	1 cut(s)	260
XagI	CCTNNNNNAGG	1 cut(s)	902
XapI	RAATTY	4 cut(s)	52, 941, 966, 1024
XbaI	TCTAGA	1 cut(s)	188
XceI	RCATGY	1 cut(s)	245
XmnI	GAANNNNTTC	1 cut(s)	509
XspI	CTAG	4 cut(s)	189, 498, 540, 1532
ZrmI	AGTACT	1 cut(s)	496
Zsp2I	ATGCAT	1 cut(s)	1219

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.