Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp02

Orthogroup ID

OG0013559

Physical Location & Seq

Genomic Coordinates Forward (+)

30292770 .. 30297772

Gene Structure 5003 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.2G329700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGATGGCCACAGCTGCTGTTATTGGGCTTAGTACGGGGAAGAGGCTGTTGAATTCTTCCTTCTATTATTCAGATATCACAGAAAAGCTCTTTCATCTCAATGATCATCATGGGTTTTTACATTGCCATTTCTCTTCAACAAAGAATGTTGTCACTGCCAGAAAGCCTTCCAATTGCAGTTCCAGATTTCCATCTTCGAATCGACCTCAACAGTCCATTAGGGCTCTGAAAGAACATGTACAAACTGCCTCTGCTCCTTCATCAGCCACGCAGCAATGGTTTCAGGAGCTCAACGACTTGGAGGAGGAAAGCTCTGATCTTGAATATTCAGTGGAAGCACTTCTTTTGCTACAGAAGTCTATGCTGGAAAAACAATGGAATCTTTCTTTTGAGAAAAAAGTATTAAGGGATTCAACATCAAAAACAACTGACAAGAAGATACCAGTCACTTGTTCTGGGGTATCTGCTCGTCAAAGGAGGATGACTAGTACCAAGAGGAAAACTTTTAACAAAAGCACTTGCGTAATGCACCCTAGCACCAATAAGCCTTTGAGAGCCATTGTCAGTCCGGAGCTGCTTCAGAATCATGTAAAGGGTTATGTGAAGGGAGTTCTAAGTGAGGATTTGCTGACTCACACTGAGGTTGTACGCCTGTCAAAGAAAATTAAAGTTGGTCTTTCCGTAGAGGAGCATAAATCAAGACTGAAGGAGAGATTGGGATGTGAGCCCTCTGATGAACAACTTGCAACTTCCTTGAGGATTTCCCGTGCTCAATTACAGTCGAAGTTGATTGAATGTAAATTGGCAAGAGAGAAGCTGGCAATGAGCAATGTTCGTTTGGTCATGTCCATTGCTCAGAGATATGATAATATGGGCGCTGAAATGGCTGACCTTATCCAGGGTGGGTTAATCGGACTACTGCGTGGGATTGAGAAATTTGATTCTTCAAAGGGGTTCAAAATTTCTACATATGTTTACTGGTGGATACGTCAGGGTGTTTCAAGAGCACTGGTTGAAAATTCCAGAACCTTAAGACTGCCTACACATTTGCATGAGAGGTTAGGTTTAATCAGAAATGCAAAAATTAGATTGGAAGAGAAAGGAATAACTCCATCAATTGATAGGATCGCAGAAAGCCTGAACATGTCCAAAAAGAAAGTTAGGAATGCTACAGAGGCAATCAGTAAGGTTTTCTCACTTGATAGGGACGCATTCCCCTCTTTAAATGGCCTTCCAGGAGAAACTCATCATAGTTACATTGCAGATAATCGCCTTGAGAACATCCCATGGCATGGGGTGGATGCGTGGGCATTGAAGGAAGAAGTAAGCAAGCTCATAAATATGATGCTTGGGGAACGGGAGAAAGAGATCATACAGCTTTATTATGGTCTGGAAAATGAATGTCTTACTTGGGAAGACATTAGTAAACGTATAGGATTGTCCAGAGAAAGAGTTAGGCAAGTTGGACTTGTTGCTCTAGAGAAACTGAAACATGCTGCGAGGAAAAGAGAGATGGAGGCCATGCTACTGAAACATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000988 GO:0000990 GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006351 GO:0006352 GO:0006355 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009507 GO:0009536 GO:0009628 GO:0009889 GO:0009987 GO:0010109 GO:0010467 GO:0010468 GO:0010556 GO:0016070 GO:0016987 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031326 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051171 GO:0051252 GO:0051716 GO:0051775 GO:0060255 GO:0065007 GO:0071214 GO:0071461 GO:0071478 GO:0071482 GO:0071704 GO:0080005 GO:0080090 GO:0090304 GO:0097659 GO:0104004 GO:0140110 GO:1901360 GO:1901362 GO:1901576 GO:1903506 GO:2000112 GO:2000142 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sigma70_r2 Sigma70_r3 Sigma70_r4

Protein Analysis

513

Amino Acids

58.26

Weight (kDa)

9.61

Isoelectric Point (pI)

44.2

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013559)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64860 AT1G64860
fragaria_vesca	FvH4_7g34280
malus_domestica	MD01G1240100.v1.1 MD07G1311500.v1.1
prunus_persica	Prupe.2G329700_v2.0.a1 Prupe.2G329700_v2.0.a1
pyrus_communis	pycom01g24680 pycom07g28370
rosa_chinensis	RchiOBHm_Chr1g0384631
rosa_laevigata	RLG00000026025
rosa_multiflora	Rmu_sc0012136.1_g000027
rosa_roxburghii	Rroxscaffold_4G00276510
rosa_samantha	Rh1AG474800 Rh1BG432900 Rh1CG446100 Rh1DG465500
rosa_wichuraiana	Rw1G039780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1292
AccIII	TCCGGA	1 cut(s)	568
AclWI	GGATC	1 cut(s)	1133
AcoI	YGGCCR	1 cut(s)	6
AcsI	RAATTY	4 cut(s)	52, 935, 960, 1018
AcuI	CTGAAG	2 cut(s)	563, 725
AfaI	GTAC	4 cut(s)	34, 240, 490, 648
AfiI	CCNNNNNNNGG	2 cut(s)	898, 1292
AflII	CTTAAG	1 cut(s)	1030
AflIII	ACRYGT	2 cut(s)	235, 1143
AhlI	ACTAGT	1 cut(s)	485
AjnI	CCWGG	2 cut(s)	897, 1234
AjuI	GAANNNNNNNTTGG	2 cut(s)	698, 730
AluBI	AGCT	8 cut(s)	14, 88, 289, 312, 574, 817, 1333, 1378
AluI	AGCT	8 cut(s)	14, 88, 289, 312, 574, 817, 1333, 1378
Alw21I	GWGCWC	3 cut(s)	291, 772, 1009
AlwI	GGATC	1 cut(s)	1133
AlwNI	CAGNNNCTG	1 cut(s)	17
Aor13HI	TCCGGA	1 cut(s)	568
AoxI	GGCC	3 cut(s)	6, 1228, 1518
ApeKI	GCWGC	4 cut(s)	14, 271, 574, 1496
ApoI	RAATTY	4 cut(s)	52, 935, 960, 1018
Asp700I	GAANNNNTTC	2 cut(s)	166, 339
AspLEI	GCGC	1 cut(s)	878
AsuII	TTCGAA	1 cut(s)	197
BalI	TGGCCA	1 cut(s)	8
BanII	GRGCYC	3 cut(s)	226, 291, 729
BbsI	GAAGAC	1 cut(s)	1422
Bbv12I	GWGCWC	3 cut(s)	291, 772, 1009
BbvI	GCAGC	3 cut(s)	283, 561, 1483
BccI	CCATC	3 cut(s)	199, 1120, 1507
BciT130I	CCWGG	2 cut(s)	899, 1236
BciVI	GTATCC	1 cut(s)	978
BclI	TGATCA	1 cut(s)	103
BcuI	ACTAGT	1 cut(s)	485
BfaI	CTAG	3 cut(s)	486, 534, 1478
BfmI	CTRYAG	2 cut(s)	350, 1170
BfoI	RGCGCY	1 cut(s)	879
BfrI	CTTAAG	1 cut(s)	1030
BfuI	GTATCC	1 cut(s)	978
BisI	GCNGC	4 cut(s)	15, 272, 575, 1497
BlsI	GCNGC	4 cut(s)	16, 273, 576, 1498
Bme1390I	CCNGG	2 cut(s)	899, 1236
BmrFI	CCNGG	2 cut(s)	899, 1236
BmsI	GCATC	2 cut(s)	1291, 1335
BpiI	GAAGAC	1 cut(s)	1422
BplI	GAGNNNNNCTC	2 cut(s)	296, 328
Bpu14I	TTCGAA	1 cut(s)	197
BpuEI	CTTGAG	2 cut(s)	775, 1295
BsaBI	GATNNNNATC	1 cut(s)	190
BsaJI	CCNNGG	2 cut(s)	898, 1286
BsaWI	WCCGGW	1 cut(s)	568
Bsc4I	CCNNNNNNNGG	2 cut(s)	898, 1292
Bse1I	ACTGG	3 cut(s)	443, 983, 1014
Bse3DI	GCAATG	6 cut(s)	121, 281, 828, 835, 849, 1257
Bse8I	GATNNNNATC	1 cut(s)	190
BseAI	TCCGGA	1 cut(s)	568
BseBI	CCWGG	2 cut(s)	899, 1236
BseDI	CCNNGG	2 cut(s)	898, 1286
BseGI	GGATG	4 cut(s)	486, 725, 1281, 1306
BseJI	GATNNNNATC	1 cut(s)	190
BseLI	CCNNNNNNNGG	2 cut(s)	898, 1292
BseMI	GCAATG	6 cut(s)	121, 281, 828, 835, 849, 1257
BseMII	CTCAG	2 cut(s)	630, 869
BseNI	ACTGG	3 cut(s)	443, 983, 1014
BseRI	GAGGAG	2 cut(s)	317, 701
BseXI	GCAGC	3 cut(s)	283, 561, 1483
BshFI	GGCC	3 cut(s)	8, 1230, 1520
BsiHKAI	GWGCWC	3 cut(s)	291, 772, 1009
BsiSI	CCGG	1 cut(s)	569
BslFI	GGGAC	1 cut(s)	1220
BslI	CCNNNNNNNGG	2 cut(s)	898, 1292
BsmFI	GGGAC	1 cut(s)	1220
BsmI	GAATGC	2 cut(s)	1171, 1211
BsnI	GGCC	3 cut(s)	8, 1230, 1520
Bsp119I	TTCGAA	1 cut(s)	197
Bsp1286I	GDGCHC	5 cut(s)	226, 291, 729, 772, 1009
Bsp13I	TCCGGA	1 cut(s)	568
Bsp1407I	TGTACA	1 cut(s)	238
Bsp143I	GATC	4 cut(s)	103, 316, 1125, 1368
Bsp19I	CCATGG	1 cut(s)	1286
BspANI	GGCC	3 cut(s)	8, 1230, 1520
BspCNI	CTCAG	2 cut(s)	631, 868
BspEI	TCCGGA	1 cut(s)	568
BspPI	GGATC	1 cut(s)	1133
BspT104I	TTCGAA	1 cut(s)	197
BspTI	CTTAAG	1 cut(s)	1030
BsrDI	GCAATG	6 cut(s)	121, 281, 828, 835, 849, 1257
BsrGI	TGTACA	1 cut(s)	238
BsrI	ACTGG	3 cut(s)	443, 983, 1014
BssECI	CCNNGG	2 cut(s)	898, 1286
BssMI	GATC	4 cut(s)	103, 316, 1125, 1368
BssT1I	CCWWGG	1 cut(s)	1286
Bst2UI	CCWGG	2 cut(s)	899, 1236
Bst4CI	ACNGT	2 cut(s)	213, 780
Bst6I	CTCTTC	3 cut(s)	35, 139, 1089
BstAFI	CTTAAG	1 cut(s)	1030
BstAUI	TGTACA	1 cut(s)	238
BstBI	TTCGAA	1 cut(s)	197
BstC8I	GCNNGC	2 cut(s)	819, 1331
BstDEI	CTNAG	4 cut(s)	29, 614, 639, 855
BstDSI	CCRYGG	1 cut(s)	1286
BstENI	CCTNNNNNAGG	1 cut(s)	896
BstF5I	GGATG	4 cut(s)	486, 725, 1281, 1306
BstH2I	RGCGCY	1 cut(s)	879
BstHHI	GCGC	1 cut(s)	878
BstKTI	GATC	4 cut(s)	106, 319, 1128, 1371
BstMBI	GATC	4 cut(s)	103, 316, 1125, 1368
BstMWI	GCNNNNNNNGC	3 cut(s)	14, 884, 1175
BstNI	CCWGG	2 cut(s)	899, 1236
BstNSI	RCATGY	3 cut(s)	239, 1147, 1496
BstSCI	CCNGG	2 cut(s)	897, 1234
BstSFI	CTRYAG	2 cut(s)	350, 1170
BstV1I	GCAGC	3 cut(s)	283, 561, 1483
BstV2I	GAAGAC	1 cut(s)	1422
BsuI	GTATCC	1 cut(s)	978
BsuRI	GGCC	3 cut(s)	8, 1230, 1520
BtgI	CCRYGG	1 cut(s)	1286
BtsCI	GGATG	4 cut(s)	486, 725, 1281, 1306
BtsI	GCAGTG	1 cut(s)	153
BtsIMutI	CAGTG	4 cut(s)	153, 336, 636, 1007
Cac8I	GCNNGC	2 cut(s)	819, 1331
CaiI	CAGNNNCTG	1 cut(s)	17
CfoI	GCGC	1 cut(s)	878
CseI	GACGC	1 cut(s)	1217
Csp6I	GTAC	4 cut(s)	33, 239, 489, 647
CviQI	GTAC	4 cut(s)	33, 239, 489, 647
DdeI	CTNAG	4 cut(s)	29, 614, 639, 855
DpnI	GATC	4 cut(s)	105, 318, 1127, 1370
DpnII	GATC	4 cut(s)	103, 316, 1125, 1368
DraI	TTTAAA	1 cut(s)	1224
EaeI	YGGCCR	1 cut(s)	6
Eam1104I	CTCTTC	3 cut(s)	35, 139, 1089
EarI	CTCTTC	3 cut(s)	35, 139, 1089
Ecl136II	GAGCTC	1 cut(s)	289
Eco130I	CCWWGG	1 cut(s)	1286
Eco24I	GRGCYC	3 cut(s)	226, 291, 729
Eco32I	GATATC	1 cut(s)	76
Eco53kI	GAGCTC	1 cut(s)	289
Eco57I	CTGAAG	2 cut(s)	563, 725
EcoICRI	GAGCTC	1 cut(s)	289
EcoNI	CCTNNNNNAGG	1 cut(s)	896
EcoRI	GAATTC	1 cut(s)	52
EcoRII	CCWGG	2 cut(s)	897, 1234
EcoRV	GATATC	1 cut(s)	76
EcoT14I	CCWWGG	1 cut(s)	1286
EcoT38I	GRGCYC	3 cut(s)	226, 291, 729
ErhI	CCWWGG	1 cut(s)	1286
FalI	AAGNNNNNCTT	6 cut(s)	327, 359, 660, 692, 1452, 1484
FaqI	GGGAC	1 cut(s)	1220
FauNDI	CATATG	1 cut(s)	970
FbaI	TGATCA	1 cut(s)	103
Fnu4HI	GCNGC	4 cut(s)	15, 272, 575, 1497
FokI	GGATG	4 cut(s)	493, 732, 1268, 1313
FriOI	GRGCYC	3 cut(s)	226, 291, 729
Fsp4HI	GCNGC	4 cut(s)	15, 272, 575, 1497
FspBI	CTAG	3 cut(s)	486, 534, 1478
GlaI	GCGC	1 cut(s)	877
GluI	GCNGC	4 cut(s)	15, 272, 575, 1497
HaeII	RGCGCY	1 cut(s)	879
HaeIII	GGCC	3 cut(s)	8, 1230, 1520
HapII	CCGG	1 cut(s)	569
HgaI	GACGC	1 cut(s)	1217
HhaI	GCGC	1 cut(s)	878
Hin6I	GCGC	1 cut(s)	876
HinP1I	GCGC	1 cut(s)	876
HinfI	GANTC	6 cut(s)	199, 379, 410, 583, 631, 941
HpaII	CCGG	1 cut(s)	569
Hpy166II	GTNNAC	2 cut(s)	976, 1427
Hpy188I	TCNGA	8 cut(s)	73, 228, 316, 582, 733, 858, 914, 1073
Hpy8I	GTNNAC	2 cut(s)	976, 1427
HpyAV	CCTTC	7 cut(s)	70, 177, 267, 598, 700, 1241, 1309
HpyCH4III	ACNGT	2 cut(s)	213, 780
HpyCH4IV	ACGT	2 cut(s)	988, 1429
HpyCH4V	TGCA	6 cut(s)	177, 529, 746, 1051, 1079, 1262
HpyF10VI	GCNNNNNNNGC	3 cut(s)	14, 884, 1175
HpyF3I	CTNAG	4 cut(s)	29, 614, 639, 855
HpySE526I	ACGT	2 cut(s)	988, 1429
HspAI	GCGC	1 cut(s)	876
Kpn2I	TCCGGA	1 cut(s)	568
Ksp22I	TGATCA	1 cut(s)	103
Kzo9I	GATC	4 cut(s)	103, 316, 1125, 1368
LmnI	GCTCC	4 cut(s)	259, 286, 571, 688
Lsp1109I	GCAGC	3 cut(s)	283, 561, 1483
LweI	GCATC	2 cut(s)	1291, 1335
MaeI	CTAG	3 cut(s)	486, 534, 1478
MaeII	ACGT	2 cut(s)	988, 1429
MaeIII	GTNAC	3 cut(s)	151, 445, 1253
MalI	GATC	4 cut(s)	105, 318, 1127, 1370
MboI	GATC	4 cut(s)	103, 316, 1125, 1368
MboII	GAAGA	9 cut(s)	48, 52, 126, 186, 448, 936, 1106, 1331, 1427
MfeI	CAATTG	2 cut(s)	172, 1116
MhlI	GDGCHC	5 cut(s)	226, 291, 729, 772, 1009
MlsI	TGGCCA	1 cut(s)	8
MluNI	TGGCCA	1 cut(s)	8
MlyI	GAGTC	1 cut(s)	625
MmeI	TCCRAC	1 cut(s)	1444
Mox20I	TGGCCA	1 cut(s)	8
MroI	TCCGGA	1 cut(s)	568
MroXI	GAANNNNTTC	2 cut(s)	166, 339
MscI	TGGCCA	1 cut(s)	8
MseI	TTAA	8 cut(s)	404, 507, 666, 908, 1031, 1067, 1223, 1537
MslI	CAYNNNNRTG	3 cut(s)	99, 1050, 1340
Msp20I	TGGCCA	1 cut(s)	8
MspA1I	CMGCKG	1 cut(s)	14
MspCI	CTTAAG	1 cut(s)	1030
MspI	CCGG	1 cut(s)	569
MspR9I	CCNGG	2 cut(s)	899, 1236
MunI	CAATTG	2 cut(s)	172, 1116
Mva1269I	GAATGC	2 cut(s)	1171, 1211
MvaI	CCWGG	2 cut(s)	899, 1236
MwoI	GCNNNNNNNGC	3 cut(s)	14, 884, 1175
NcoI	CCATGG	1 cut(s)	1286
NdeI	CATATG	1 cut(s)	970
NdeII	GATC	4 cut(s)	103, 316, 1125, 1368
NmuCI	GTSAC	2 cut(s)	151, 445
NspI	RCATGY	3 cut(s)	239, 1147, 1496
NspV	TTCGAA	1 cut(s)	197
PciI	ACATGT	2 cut(s)	235, 1143
PctI	GAATGC	2 cut(s)	1171, 1211
PdmI	GAANNNNTTC	2 cut(s)	166, 339
PfeI	GAWTC	5 cut(s)	199, 379, 410, 583, 941
PflMI	CCANNNNNTGG	1 cut(s)	1292
PfoI	TCCNGGA	1 cut(s)	1234
PkrI	GCNGC	4 cut(s)	16, 273, 576, 1498
PleI	GAGTC	1 cut(s)	625
PpsI	GAGTC	1 cut(s)	625
PscI	ACATGT	2 cut(s)	235, 1143
Psp124BI	GAGCTC	1 cut(s)	291
Psp6I	CCWGG	2 cut(s)	897, 1234
PspGI	CCWGG	2 cut(s)	897, 1234
PstNI	CAGNNNCTG	1 cut(s)	17
PvuII	CAGCTG	1 cut(s)	14
RsaI	GTAC	4 cut(s)	34, 240, 490, 648
RsaNI	GTAC	4 cut(s)	33, 239, 489, 647
RseI	CAYNNNNRTG	3 cut(s)	99, 1050, 1340
SacI	GAGCTC	1 cut(s)	291
SaqAI	TTAA	8 cut(s)	404, 507, 666, 908, 1031, 1067, 1223, 1537
SatI	GCNGC	4 cut(s)	15, 272, 575, 1497
Sau3AI	GATC	4 cut(s)	103, 316, 1125, 1368
SchI	GAGTC	1 cut(s)	625
ScrFI	CCNGG	2 cut(s)	899, 1236
SduI	GDGCHC	5 cut(s)	226, 291, 729, 772, 1009
SfaNI	GCATC	2 cut(s)	1291, 1335
SfcI	CTRYAG	2 cut(s)	350, 1170
SfuI	TTCGAA	1 cut(s)	197
SmiMI	CAYNNNNRTG	3 cut(s)	99, 1050, 1340
SmlI	CTYRAG	3 cut(s)	754, 1030, 1274
SmoI	CTYRAG	3 cut(s)	754, 1030, 1274
SpeI	ACTAGT	1 cut(s)	485
SspI	AATATT	1 cut(s)	326
SspMI	CTAG	3 cut(s)	486, 534, 1478
SstI	GAGCTC	1 cut(s)	291
StyD4I	CCNGG	2 cut(s)	897, 1234
StyI	CCWWGG	1 cut(s)	1286
TaaI	ACNGT	2 cut(s)	213, 780
TaiI	ACGT	2 cut(s)	991, 1432
TaqI	TCGA	3 cut(s)	197, 202, 782
TatI	WGTACW	1 cut(s)	238
TfiI	GAWTC	5 cut(s)	199, 379, 410, 583, 941
Tru1I	TTAA	8 cut(s)	404, 507, 666, 908, 1031, 1067, 1223, 1537
Tru9I	TTAA	8 cut(s)	404, 507, 666, 908, 1031, 1067, 1223, 1537
TscAI	CASTG	4 cut(s)	160, 336, 643, 1014
TseFI	GTSAC	2 cut(s)	151, 445
TseI	GCWGC	4 cut(s)	14, 271, 574, 1496
Tsp45I	GTSAC	2 cut(s)	151, 445
TspDTI	ATGAA	4 cut(s)	83, 249, 750, 1413
TspGWI	ACGGA	1 cut(s)	670
TspRI	CASTG	4 cut(s)	160, 336, 643, 1014
Van91I	CCANNNNNTGG	1 cut(s)	1292
Vha464I	CTTAAG	1 cut(s)	1030
XagI	CCTNNNNNAGG	1 cut(s)	896
XapI	RAATTY	4 cut(s)	52, 935, 960, 1018
XbaI	TCTAGA	1 cut(s)	1477
XceI	RCATGY	3 cut(s)	239, 1147, 1496
XmnI	GAANNNNTTC	2 cut(s)	166, 339
XspI	CTAG	3 cut(s)	486, 534, 1478

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.