MD02G1009600.v1.1

CCR4-associated factor 1 homolog 7

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016481
Physical Location & Seq
Forward (+)
639547 .. 641050
1504 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1009600.v1.1.491

Sequence Viewer

Length: 825 bp
ATGTCTAGTTTTCCGAATAGAGATTCGATTCACATTAGGGAAGTTTGGAGCGATAATCTTGAGGAGGAGTTTAAATTGATTCGCAAAGTTGTAGAGGAATACCGATATGTAGCAATGGACACAGAGTTTCCCGGTATTGTTTTGAGGCCTGTAGGGACTTTCAAGGACAGTTATGATTATCACTATCAAACCCTTAAGGCCAATGTGGACCTCTTGAAGTTGATACAGTTGGGTCTCACATTTTCTGATGAGAAAGGGAACCTTCCGACCTGTGGAACCGACAAGCACTGTGTGTGGCAATTCAATTTCCGCGACTTCAACCCCAACGAGGATGTGTATGCTAGTGACTCGATAGAGTTACTGTCCCAGAGCGGTATGGATTTTGTGAAGAACAATGAGAAGGGTGTTGATGCTAGTAAGTTCACCGAGCTACTAATGACATCCGGAGTTGTGCTGTCTGATAATGTGGTTTGGGTGACATTCCATAGTGGGTATGATTTTGGGTACTTGCTCAAGCTGCTTACAGGCCAAAGCCTCCCGAACACGCAGGCGGGGTTCTTTAAAATGATCAAGATGTATTTTCCTACAGTTTATGATATCAAGCATCTGATGAGGTTCTGCAACAGCCTTCATGGTGGGTTGAACAAACTCGCGGAGCTGTTAGATGTTGAGAGAATTGGCATCTCCCACCAAGCTGGTTCGGATAGTTTGCTAACATGCAGTACGTTCATGAAACTGAAAGAGACCTACTTTGATGGGTCTCCTGACAAATATGCTGGTGTTTTATATGGTCTTGGTGTTGAGAATGGACCTAGTTCTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

275

Amino Acids

31.07

Weight (kDa)

5.17

Isoelectric Point (pI)

33.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 15 - 136 9.8e-15 CAF1 family ribonuclease
CAF1 PF04857 155 - 217 7.2e-06 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01160 FvH4_1g01160
malus_domestica MD02G1009600.v1.1
prunus_persica Prupe.7G260400_v2.0.a1
pyrus_communis pycom15g13870
rosa_chinensis RchiOBHm_Chr2g0085951
rosa_laevigata RLG00000015729
rosa_roxburghii Rroxscaffold_2G00154900
rosa_rugosa Rorug01G0464600
rosa_samantha Rh2AG013800 Rh2BG014300 Rh2CG014800 Rh2DG016100
rosa_wichuraiana Rw2G001220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 372
AccII CGCG 2 cut(s) 312, 653
AccIII TCCGGA 1 cut(s) 443
AciI CCGC 4 cut(s) 310, 372, 551, 653
AdeI CACNNNGTG 1 cut(s) 292
AfaI GTAC 2 cut(s) 506, 724
AfiI CCNNNNNNNGG 2 cut(s) 272, 328
AflII CTTAAG 1 cut(s) 194
AgsI TTSAA 5 cut(s) 163, 217, 304, 319, 643
AluBI AGCT 4 cut(s) 430, 517, 658, 695
AluI AGCT 4 cut(s) 430, 517, 658, 695
Alw26I GTCTC 3 cut(s) 239, 737, 765
Aor13HI TCCGGA 1 cut(s) 443
AoxI GGCC 3 cut(s) 146, 198, 526
ApeKI GCWGC 1 cut(s) 517
AspS9I GGNCC 2 cut(s) 208, 809
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 2 cut(s) 415, 487
AvaII GGWCC 2 cut(s) 208, 809
BbvI GCAGC 1 cut(s) 504
BccI CCATC 1 cut(s) 749
BclI TGATCA 1 cut(s) 567
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 3 cut(s) 239, 737, 765
BfaI CTAG 4 cut(s) 6, 342, 414, 813
BfmI CTRYAG 2 cut(s) 150, 585
BfrI CTTAAG 1 cut(s) 194
BisI GCNGC 1 cut(s) 518
BlsI GCNGC 1 cut(s) 519
Bme1390I CCNGG 1 cut(s) 132
Bme18I GGWCC 2 cut(s) 208, 809
BmgT120I GGNCC 2 cut(s) 208, 809
BmiI GGNNCC 2 cut(s) 260, 277
BmrFI CCNGG 1 cut(s) 132
BmsI GCATC 3 cut(s) 400, 613, 690
BpuEI CTTGAG 2 cut(s) 80, 497
BpuMI CCSGG 1 cut(s) 132
BsaI GGTCTC 3 cut(s) 239, 737, 765
BsaWI WCCGGW 1 cut(s) 443
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 328
Bse3DI GCAATG 1 cut(s) 120
BseAI TCCGGA 1 cut(s) 443
BseGI GGATG 2 cut(s) 337, 440
BseLI CCNNNNNNNGG 2 cut(s) 272, 328
BseMI GCAATG 1 cut(s) 120
BseRI GAGGAG 2 cut(s) 77, 80
BseXI GCAGC 1 cut(s) 504
Bsh1236I CGCG 2 cut(s) 312, 653
BshFI GGCC 3 cut(s) 148, 200, 528
BsiSI CCGG 2 cut(s) 132, 444
BslFI GGGAC 2 cut(s) 169, 349
BslI CCNNNNNNNGG 2 cut(s) 272, 328
BsmAI GTCTC 3 cut(s) 239, 737, 765
BsmFI GGGAC 2 cut(s) 169, 349
BsnI GGCC 3 cut(s) 148, 200, 528
Bso31I GGTCTC 3 cut(s) 239, 737, 765
Bsp13I TCCGGA 1 cut(s) 443
Bsp143I GATC 1 cut(s) 567
BspACI CCGC 4 cut(s) 310, 372, 551, 653
BspANI GGCC 3 cut(s) 148, 200, 528
BspEI TCCGGA 1 cut(s) 443
BspFNI CGCG 2 cut(s) 312, 653
BspHI TCATGA 1 cut(s) 729
BspLI GGNNCC 2 cut(s) 260, 277
BspTI CTTAAG 1 cut(s) 194
BspTNI GGTCTC 3 cut(s) 239, 737, 765
BsrBI CCGCTC 1 cut(s) 372
BsrDI GCAATG 1 cut(s) 120
BssMI GATC 1 cut(s) 567
Bst4CI ACNGT 5 cut(s) 170, 228, 290, 363, 589
BstAFI CTTAAG 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 549
BstF5I GGATG 2 cut(s) 337, 440
BstFNI CGCG 2 cut(s) 312, 653
BstKTI GATC 1 cut(s) 570
BstMAI GTCTC 3 cut(s) 239, 737, 765
BstMBI GATC 1 cut(s) 567
BstMWI GCNNNNNNNGC 1 cut(s) 517
BstNSI RCATGY 1 cut(s) 720
BstSCI CCNGG 1 cut(s) 130
BstSFI CTRYAG 2 cut(s) 150, 585
BstUI CGCG 2 cut(s) 312, 653
BstV1I GCAGC 1 cut(s) 504
BstXI CCANNNNNNTGG 1 cut(s) 695
BsuRI GGCC 3 cut(s) 148, 200, 528
BtsCI GGATG 2 cut(s) 337, 440
BtsIMutI CAGTG 1 cut(s) 286
Cac8I GCNNGC 1 cut(s) 549
CciI TCATGA 1 cut(s) 729
Cfr13I GGNCC 2 cut(s) 208, 809
Csp6I GTAC 2 cut(s) 505, 723
CviAII CATG 3 cut(s) 632, 717, 730
CviJI RGCY 9 cut(s) 148, 200, 430, 517, 528, 534, 627, 658, 695
CviKI_1 RGCY 9 cut(s) 148, 200, 430, 517, 528, 534, 627, 658, 695
CviQI GTAC 2 cut(s) 505, 723
DpnI GATC 1 cut(s) 569
DpnII GATC 1 cut(s) 567
DraI TTTAAA 2 cut(s) 73, 562
DraIII CACNNNGTG 1 cut(s) 292
Eco147I AGGCCT 1 cut(s) 148
Eco31I GGTCTC 3 cut(s) 239, 737, 765
Eco32I GATATC 1 cut(s) 598
Eco47I GGWCC 2 cut(s) 208, 809
EcoRV GATATC 1 cut(s) 598
FaeI CATG 3 cut(s) 635, 720, 733
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FaqI GGGAC 2 cut(s) 169, 349
FatI CATG 3 cut(s) 631, 716, 729
FauI CCCGC 1 cut(s) 544
FbaI TGATCA 1 cut(s) 567
Fnu4HI GCNGC 1 cut(s) 518
FokI GGATG 2 cut(s) 344, 427
Fsp4HI GCNGC 1 cut(s) 518
FspBI CTAG 4 cut(s) 6, 342, 414, 813
GluI GCNGC 1 cut(s) 518
HaeIII GGCC 3 cut(s) 148, 200, 528
HapII CCGG 2 cut(s) 132, 444
Hin1II CATG 3 cut(s) 635, 720, 733
HinfI GANTC 4 cut(s) 23, 28, 79, 347
HpaII CCGG 2 cut(s) 132, 444
HphI GGTGA 2 cut(s) 415, 487
Hpy166II GTNNAC 2 cut(s) 208, 423
Hpy188I TCNGA 6 cut(s) 15, 247, 267, 460, 609, 703
Hpy188III TCNNGA 7 cut(s) 59, 214, 444, 538, 571, 730, 764
Hpy8I GTNNAC 2 cut(s) 208, 423
HpyAV CCTTC 3 cut(s) 272, 394, 638
HpyCH4III ACNGT 5 cut(s) 170, 228, 290, 363, 589
HpyCH4IV ACGT 1 cut(s) 725
HpyCH4V TGCA 2 cut(s) 621, 720
HpyF10VI GCNNNNNNNGC 1 cut(s) 517
HpySE526I ACGT 1 cut(s) 725
Hsp92II CATG 3 cut(s) 635, 720, 733
Kpn2I TCCGGA 1 cut(s) 443
Ksp22I TGATCA 1 cut(s) 567
Kzo9I GATC 1 cut(s) 567
LmnI GCTCC 2 cut(s) 48, 655
Lsp1109I GCAGC 1 cut(s) 504
LweI GCATC 3 cut(s) 400, 613, 690
MaeI CTAG 4 cut(s) 6, 342, 414, 813
MaeII ACGT 1 cut(s) 725
MaeIII GTNAC 3 cut(s) 344, 357, 475
MalI GATC 1 cut(s) 569
MbiI CCGCTC 1 cut(s) 372
MboI GATC 1 cut(s) 567
MboII GAAGA 1 cut(s) 400
MluCI AATT 4 cut(s) 74, 299, 304, 675
MlyI GAGTC 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 290
MnlI CCTC 8 cut(s) 55, 58, 88, 138, 221, 322, 545, 606
MroI TCCGGA 1 cut(s) 443
MseI TTAA 3 cut(s) 72, 195, 561
MspCI CTTAAG 1 cut(s) 194
MspI CCGG 2 cut(s) 132, 444
MspR9I CCNGG 1 cut(s) 132
MvnI CGCG 2 cut(s) 312, 653
MwoI GCNNNNNNNGC 1 cut(s) 517
NciI CCSGG 1 cut(s) 132
NdeII GATC 1 cut(s) 567
NlaIII CATG 3 cut(s) 635, 720, 733
NlaIV GGNNCC 2 cut(s) 260, 277
NmuCI GTSAC 2 cut(s) 344, 475
NspI RCATGY 1 cut(s) 720
PagI TCATGA 1 cut(s) 729
PceI AGGCCT 1 cut(s) 148
PfeI GAWTC 3 cut(s) 23, 28, 79
PkrI GCNGC 1 cut(s) 519
PleI GAGTC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 341
PspN4I GGNNCC 2 cut(s) 260, 277
PspPI GGNCC 2 cut(s) 208, 809
RsaI GTAC 2 cut(s) 506, 724
RsaNI GTAC 2 cut(s) 505, 723
SaqAI TTAA 3 cut(s) 72, 195, 561
SatI GCNGC 1 cut(s) 518
Sau3AI GATC 1 cut(s) 567
Sau96I GGNCC 2 cut(s) 208, 809
SchI GAGTC 1 cut(s) 341
ScrFI CCNGG 1 cut(s) 132
SfaNI GCATC 3 cut(s) 400, 613, 690
SfcI CTRYAG 2 cut(s) 150, 585
SinI GGWCC 2 cut(s) 208, 809
SmlI CTYRAG 3 cut(s) 59, 194, 512
SmoI CTYRAG 3 cut(s) 59, 194, 512
Sse9I AATT 4 cut(s) 74, 299, 304, 675
SseBI AGGCCT 1 cut(s) 148
SsiI CCGC 4 cut(s) 310, 372, 551, 653
SspMI CTAG 4 cut(s) 6, 342, 414, 813
StuI AGGCCT 1 cut(s) 148
StyD4I CCNGG 1 cut(s) 130
TaaI ACNGT 5 cut(s) 170, 228, 290, 363, 589
TaiI ACGT 1 cut(s) 728
TaqI TCGA 2 cut(s) 26, 350
TasI AATT 4 cut(s) 74, 299, 304, 675
TfiI GAWTC 3 cut(s) 23, 28, 79
Tru1I TTAA 3 cut(s) 72, 195, 561
Tru9I TTAA 3 cut(s) 72, 195, 561
TscAI CASTG 1 cut(s) 293
TseFI GTSAC 2 cut(s) 344, 475
TseI GCWGC 1 cut(s) 517
Tsp45I GTSAC 2 cut(s) 344, 475
TspDTI ATGAA 3 cut(s) 620, 718, 746
TspRI CASTG 1 cut(s) 293
Vha464I CTTAAG 1 cut(s) 194
VpaK11BI GGWCC 2 cut(s) 208, 809
XceI RCATGY 1 cut(s) 720
XspI CTAG 4 cut(s) 6, 342, 414, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.