Rorug01G0464600

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0016481
Physical Location & Seq
Reverse (-)
55702461 .. 55703699
1239 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0464600.1

Sequence Viewer

Length: 1239 bp
ATGGGGAGGCTGCTGAGTATGAAAGATTTTGAGGGCAAAGCAATTCGACCTCGTTCGTCCTACCTTGATATTTTTCTTCAGGTTGCTAGTGTTCTTCAGAATAAGAAACTCAAGCAAAGTATGGAGAAAAGTACTCATTTGAAAACCAAGATGGCCGGTATTGTGTTTAAAGAACCCATTTTCATGGTAGGGAAAGAGACTTATGCAAAGGGACAAAAATTTATGAACTCTGTTTTTGAATCTGTGAGGAAGATTGGTGGGTCAAAAAGAAAGGGTTTTGACAATTTTGACGATCATGAAAGGGGTTTTCAATTCAAGAAGCAGAAGATGGATATGGGTAGTACCTCCGAGGCTAAAGAACCAAATTCGTCAGTCGTAAAAGAATTGAAGGTGGATGCGTTATACTCAGTTAGCACAATAAAGACAGAAAATTCTTTGCCTAGGGCAAACTGTATAATGAGTTTTGATATTAAGAAGAAGAGTGCTAATGGTGATGATGATAAACAATGGGGTTTGAGGCCGAAGCCGAAGAATATCAAATTGAGGAAGCCAAAGAAGGAGATCACAACACAAATTATTGATGCTAACGGCTTGCCTGAAGAATACAAGAGAAAGATTGAAAATTTTGATAAGGCTAAGGCGACTTTGGTGATACAGAAGGAGTTGACCAAGACTGATTTGTCACGTACCGCTTGCCGGTTGTCAATGCCTATGAACCAAATCAAACCGGAGAGTTTTCTGAATGCAGATGAGATAAAGTTTCTTGCAAAACAAAATGACTGGCCAGTTTCGGTTATAGATCCCCTACTTGAAGAAGAAGATCTAACCCTGAGGCAGTGGAACATGGAAAAGAAAACGGGTAAGACGAGCTCAAGCTATGTGTTGAAAACACGTTGGAATGAAGTTGCCGATAAAAACAATCTCAAGCAAAAAGATGTAGTCCAAGTTTGGTCATTCCGGGATGTGGGAAACAACCTTCACTTTGCTATTGTTTTGGTTAAGAAGGGTGAGAGAAAGGGTGGTGAACATAGTAGTAGTCGAGGGTATAGCCAGCCGCCTTCGCTATCAACAACACCGCGTGATATTGTTGATGGTGAAAGAAGAAGTGGTAGTGGTCAAGGAACAGAATCAAAGGAAGAAGGGATGATAGGAGTTAATAGCAATGTTGAGAGTAATAGTGAGAGAAGTACTATGAGCAATGGGTCTAATGAAAACCATGAAAGTTTAAGCCAGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

412

Amino Acids

46.63

Weight (kDa)

9.56

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 211 - 304 3e-16 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01160 FvH4_1g01160
malus_domestica MD02G1009600.v1.1
prunus_persica Prupe.7G260400_v2.0.a1
pyrus_communis pycom15g13870
rosa_chinensis RchiOBHm_Chr2g0085951
rosa_laevigata RLG00000015729
rosa_roxburghii Rroxscaffold_2G00154900
rosa_rugosa Rorug01G0464600
rosa_samantha Rh2AG013800 Rh2BG014300 Rh2CG014800 Rh2DG016100
rosa_wichuraiana Rw2G001220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 679
AccII CGCG 1 cut(s) 1078
AciI CCGC 3 cut(s) 690, 1055, 1076
AclWI GGATC 1 cut(s) 794
AcoI YGGCCR 2 cut(s) 153, 782
AcsI RAATTY 4 cut(s) 218, 364, 430, 622
AcuI CTGAAG 3 cut(s) 62, 80, 618
AdeI CACNNNGTG 1 cut(s) 1079
AfaI GTAC 4 cut(s) 133, 343, 688, 1189
AfiI CCNNNNNNNGG 2 cut(s) 696, 964
AflIII ACRYGT 1 cut(s) 890
AgsI TTSAA 8 cut(s) 142, 239, 311, 316, 388, 620, 812, 886
AluBI AGCT 2 cut(s) 870, 876
AluI AGCT 2 cut(s) 870, 876
Alw21I GWGCWC 1 cut(s) 872
Alw26I GTCTC 1 cut(s) 191
AlwI GGATC 1 cut(s) 794
AoxI GGCC 3 cut(s) 153, 518, 782
ApeKI GCWGC 1 cut(s) 10
ApoI RAATTY 4 cut(s) 218, 364, 430, 622
AspA2I CCTAGG 1 cut(s) 440
AsuC2I CCSGG 1 cut(s) 959
AsuHPI GGTGA 5 cut(s) 503, 661, 1019, 1034, 1106
AvrII CCTAGG 1 cut(s) 440
AxyI CCTNAGG 1 cut(s) 830
BalI TGGCCA 1 cut(s) 784
BanII GRGCYC 1 cut(s) 872
Bbv12I GWGCWC 1 cut(s) 872
BccI CCATC 3 cut(s) 145, 322, 1085
BceAI ACGGC 1 cut(s) 604
BcnI CCSGG 1 cut(s) 959
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 2 cut(s) 87, 441
BglII AGATCT 1 cut(s) 820
BisI GCNGC 2 cut(s) 11, 1055
BlnI CCTAGG 1 cut(s) 440
BlsI GCNGC 2 cut(s) 12, 1056
BmcAI AGTACT 2 cut(s) 133, 1189
Bme1390I CCNGG 1 cut(s) 959
BmrFI CCNGG 1 cut(s) 959
BmsI GCATC 2 cut(s) 385, 571
Bpu10I CCTNAGC 1 cut(s) 636
BpuEI CTTGAG 3 cut(s) 95, 856, 908
BpuMI CCSGG 1 cut(s) 959
BsaAI YACGTR 1 cut(s) 686
BsaJI CCNNGG 2 cut(s) 348, 440
BsaWI WCCGGW 1 cut(s) 727
Bsc4I CCNNNNNNNGG 2 cut(s) 696, 964
Bse118I RCCGGY 2 cut(s) 155, 696
Bse1I ACTGG 2 cut(s) 785, 785
Bse21I CCTNAGG 1 cut(s) 830
Bse3DI GCAATG 2 cut(s) 1168, 1204
BseDI CCNNGG 2 cut(s) 348, 440
BseGI GGATG 3 cut(s) 400, 967, 1149
BseLI CCNNNNNNNGG 2 cut(s) 696, 964
BseMI GCAATG 2 cut(s) 1168, 1204
BseMII CTCAG 3 cut(s) 5, 420, 821
BseNI ACTGG 2 cut(s) 785, 785
Bsh1236I CGCG 1 cut(s) 1078
BshFI GGCC 3 cut(s) 155, 520, 784
BsiHKAI GWGCWC 1 cut(s) 872
BsiSI CCGG 4 cut(s) 156, 697, 728, 958
BslFI GGGAC 1 cut(s) 225
BslI CCNNNNNNNGG 2 cut(s) 696, 964
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 225
BsmI GAATGC 1 cut(s) 748
BsnI GGCC 3 cut(s) 155, 520, 784
Bsp1286I GDGCHC 1 cut(s) 872
Bsp143I GATC 4 cut(s) 292, 561, 799, 820
BspACI CCGC 3 cut(s) 690, 1055, 1076
BspANI GGCC 3 cut(s) 155, 520, 784
BspCNI CTCAG 3 cut(s) 6, 419, 822
BspFNI CGCG 1 cut(s) 1078
BspHI TCATGA 1 cut(s) 295
BspPI GGATC 1 cut(s) 794
BsrDI GCAATG 2 cut(s) 1168, 1204
BsrFI RCCGGY 2 cut(s) 155, 696
BsrI ACTGG 2 cut(s) 785, 785
BssAI RCCGGY 2 cut(s) 155, 696
BssECI CCNNGG 2 cut(s) 348, 440
BssMI GATC 4 cut(s) 292, 561, 799, 820
BssT1I CCWWGG 1 cut(s) 440
Bst4CI ACNGT 1 cut(s) 452
Bst6I CTCTTC 1 cut(s) 473
BstBAI YACGTR 1 cut(s) 686
BstC8I GCNNGC 3 cut(s) 593, 694, 1052
BstDEI CTNAG 4 cut(s) 14, 406, 636, 830
BstF5I GGATG 3 cut(s) 400, 967, 1149
BstFNI CGCG 1 cut(s) 1078
BstKTI GATC 4 cut(s) 295, 564, 802, 823
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 4 cut(s) 292, 561, 799, 820
BstMWI GCNNNNNNNGC 1 cut(s) 1060
BstSCI CCNGG 1 cut(s) 957
BstUI CGCG 1 cut(s) 1078
BstX2I RGATCY 2 cut(s) 799, 820
BstXI CCANNNNNNTGG 1 cut(s) 184
BstYI RGATCY 2 cut(s) 799, 820
Bsu36I CCTNAGG 1 cut(s) 830
BsuRI GGCC 3 cut(s) 155, 520, 784
BtsCI GGATG 3 cut(s) 400, 967, 1149
BtsI GCAGTG 1 cut(s) 842
BtsIMutI CAGTG 1 cut(s) 842
Cac8I GCNNGC 3 cut(s) 593, 694, 1052
CciI TCATGA 1 cut(s) 295
Cfr10I RCCGGY 2 cut(s) 155, 696
Csp6I GTAC 4 cut(s) 132, 342, 687, 1188
CviAII CATG 4 cut(s) 184, 296, 844, 1217
CviQI GTAC 4 cut(s) 132, 342, 687, 1188
DdeI CTNAG 4 cut(s) 14, 406, 636, 830
DpnI GATC 4 cut(s) 294, 563, 801, 822
DpnII GATC 4 cut(s) 292, 561, 799, 820
DraI TTTAAA 1 cut(s) 169
DraIII CACNNNGTG 1 cut(s) 1079
DrdI GACNNNNNNGTC 1 cut(s) 679
DseDI GACNNNNNNGTC 1 cut(s) 679
EaeI YGGCCR 2 cut(s) 153, 782
Eam1104I CTCTTC 1 cut(s) 473
EarI CTCTTC 1 cut(s) 473
Ecl136II GAGCTC 1 cut(s) 870
Eco130I CCWWGG 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 872
Eco53kI GAGCTC 1 cut(s) 870
Eco57I CTGAAG 3 cut(s) 62, 80, 618
Eco81I CCTNAGG 1 cut(s) 830
EcoICRI GAGCTC 1 cut(s) 870
EcoT14I CCWWGG 1 cut(s) 440
EcoT38I GRGCYC 1 cut(s) 872
ErhI CCWWGG 1 cut(s) 440
FaeI CATG 4 cut(s) 187, 299, 847, 1220
FaqI GGGAC 1 cut(s) 225
FatI CATG 4 cut(s) 183, 295, 843, 1216
Fnu4HI GCNGC 2 cut(s) 11, 1055
FokI GGATG 3 cut(s) 407, 974, 1156
FriOI GRGCYC 1 cut(s) 872
Fsp4HI GCNGC 2 cut(s) 11, 1055
FspBI CTAG 2 cut(s) 87, 441
GluI GCNGC 2 cut(s) 11, 1055
HaeIII GGCC 3 cut(s) 155, 520, 784
HapII CCGG 4 cut(s) 156, 697, 728, 958
Hin1II CATG 4 cut(s) 187, 299, 847, 1220
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HinfI GANTC 2 cut(s) 239, 1127
HpaII CCGG 4 cut(s) 156, 697, 728, 958
HphI GGTGA 5 cut(s) 503, 661, 1019, 1034, 1106
Hpy166II GTNNAC 2 cut(s) 666, 1025
Hpy188I TCNGA 3 cut(s) 99, 349, 741
Hpy188III TCNNGA 2 cut(s) 296, 316
Hpy8I GTNNAC 2 cut(s) 666, 1025
HpyAV CCTTC 7 cut(s) 382, 550, 652, 986, 997, 1068, 1133
HpyCH4III ACNGT 1 cut(s) 452
HpyCH4IV ACGT 2 cut(s) 685, 892
HpyCH4V TGCA 3 cut(s) 206, 746, 767
HpyF10VI GCNNNNNNNGC 1 cut(s) 1060
HpyF3I CTNAG 4 cut(s) 14, 406, 636, 830
HpySE526I ACGT 2 cut(s) 685, 892
Hsp92II CATG 4 cut(s) 187, 299, 847, 1220
Kzo9I GATC 4 cut(s) 292, 561, 799, 820
LweI GCATC 2 cut(s) 385, 571
MaeI CTAG 2 cut(s) 87, 441
MaeII ACGT 2 cut(s) 685, 892
MaeIII GTNAC 1 cut(s) 681
MalI GATC 4 cut(s) 294, 563, 801, 822
MboI GATC 4 cut(s) 292, 561, 799, 820
MflI RGATCY 2 cut(s) 799, 820
MhlI GDGCHC 1 cut(s) 872
MlsI TGGCCA 1 cut(s) 784
MluNI TGGCCA 1 cut(s) 784
MmeI TCCRAC 1 cut(s) 875
MnlI CCTC 9 cut(s) 25, 60, 240, 343, 355, 510, 537, 825, 1034
Mox20I TGGCCA 1 cut(s) 784
MscI TGGCCA 1 cut(s) 784
MseI TTAA 5 cut(s) 168, 471, 999, 1155, 1226
MslI CAYNNNNRTG 1 cut(s) 182
Msp20I TGGCCA 1 cut(s) 784
MspI CCGG 4 cut(s) 156, 697, 728, 958
MspR9I CCNGG 1 cut(s) 959
Mva1269I GAATGC 1 cut(s) 748
MvnI CGCG 1 cut(s) 1078
MwoI GCNNNNNNNGC 1 cut(s) 1060
NciI CCSGG 1 cut(s) 959
NdeII GATC 4 cut(s) 292, 561, 799, 820
NlaIII CATG 4 cut(s) 187, 299, 847, 1220
NmuCI GTSAC 1 cut(s) 681
PagI TCATGA 1 cut(s) 295
PcsI WCGNNNNNNNCGW 1 cut(s) 863
PctI GAATGC 1 cut(s) 748
PfeI GAWTC 2 cut(s) 239, 1127
PfoI TCCNGGA 1 cut(s) 957
PkrI GCNGC 2 cut(s) 12, 1056
Ppu21I YACGTR 1 cut(s) 686
Psp124BI GAGCTC 1 cut(s) 872
PsuI RGATCY 2 cut(s) 799, 820
RsaI GTAC 4 cut(s) 133, 343, 688, 1189
RsaNI GTAC 4 cut(s) 132, 342, 687, 1188
RseI CAYNNNNRTG 1 cut(s) 182
SacI GAGCTC 1 cut(s) 872
SaqAI TTAA 5 cut(s) 168, 471, 999, 1155, 1226
SatI GCNGC 2 cut(s) 11, 1055
Sau3AI GATC 4 cut(s) 292, 561, 799, 820
ScaI AGTACT 2 cut(s) 133, 1189
ScrFI CCNGG 1 cut(s) 959
SduI GDGCHC 1 cut(s) 872
SfaNI GCATC 2 cut(s) 385, 571
SmiMI CAYNNNNRTG 1 cut(s) 182
SmlI CTYRAG 3 cut(s) 110, 871, 923
SmoI CTYRAG 3 cut(s) 110, 871, 923
SsiI CCGC 3 cut(s) 690, 1055, 1076
SspMI CTAG 2 cut(s) 87, 441
SstI GAGCTC 1 cut(s) 872
StyD4I CCNGG 1 cut(s) 957
StyI CCWWGG 1 cut(s) 440
TaaI ACNGT 1 cut(s) 452
TaiI ACGT 2 cut(s) 688, 895
TaqI TCGA 2 cut(s) 46, 1039
TatI WGTACW 2 cut(s) 131, 1187
TauI GCSGC 1 cut(s) 1057
TfiI GAWTC 2 cut(s) 239, 1127
Tru1I TTAA 5 cut(s) 168, 471, 999, 1155, 1226
Tru9I TTAA 5 cut(s) 168, 471, 999, 1155, 1226
TscAI CASTG 1 cut(s) 842
TseFI GTSAC 1 cut(s) 681
TseI GCWGC 1 cut(s) 10
Tsp45I GTSAC 1 cut(s) 681
TspDTI ATGAA 8 cut(s) 35, 172, 239, 312, 728, 915, 1224, 1233
TspRI CASTG 1 cut(s) 842
XapI RAATTY 4 cut(s) 218, 364, 430, 622
XmaJI CCTAGG 1 cut(s) 440
XspI CTAG 2 cut(s) 87, 441
ZrmI AGTACT 2 cut(s) 133, 1189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.