Rw2G001220

CCR4-associated factor 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016481
Physical Location & Seq
Forward (+)
1031349 .. 1032788
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G001220.1

Sequence Viewer

Length: 813 bp
ATGTCTTTTTTGTTGAAGAGTGATTCAGTTCACATTAGGGAAGTTTGGAAGGATAATCTTGATGTGGAGTTTGAATTGATTCGGAATATCGTAGACGAGTACCCTTATATAGCTATGGATACAGAGTTTCCGGGTATCGTTTTGCGTCCCTTGGGGACTTTCAAGAGCACTTTTGATTATAATTATCAGACCCTTAAGTCCAATGTAGACCTGTTGAAGTTGATTCAGCTGGGTCTCACGTTTTCGGATGAGAATGGGAAGCTGCCCACCTGTGGAACTGACAAGTACTGTGTGTGGCAGTTCAATTTCTGCAACTTTAATGTTAATGAGGATATGTATGCTGTGGATTCGCTTGAGTTATTGTCCCACAGCGGAATGGATTTCACGAAGAACAATGAGAAGGGTGTCGATGCTCGTAAGTTCACTGAGCTGTTGATGACCTCTGGCATTGTGCTGAATGAAAATGTGGTTTGGGTGACATTCCATAGTGCATATGATTTCGGCTACTTGATCAAGCTGCTTACCTGCAAAACCCTTCCAGAGTCGCAGACAGAGTTCTTTGATGCGATCAATTTGTACTTCCCGACGATTTATGATGTTAAGCATCTGATGAGGTTCTGCAACAGCCTTCATGGTGGGTTGAACAAGCTGGCAGAGCTGTTAGATGTGGAGAGAGTTGGCATGTCTCACCAAGCTGGTTCTGATAGTTTACTTACCAGTTCTACATTCATGAAATTGAAAAAGAGTTTCTTTAGTGGGTCTCCTGAGAAATATGCTGGTGTCTTGTATGGTCTTGGTATTGAGGATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03018 map03018
Pfam Domains
Protein Families
CAF1

Protein Analysis

270

Amino Acids

30.77

Weight (kDa)

4.93

Isoelectric Point (pI)

27.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CAF1 PF04857 15 - 136 1.3e-14 CAF1 family ribonuclease
CAF1 PF04857 154 - 217 2e-06 CAF1 family ribonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016481)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01160 FvH4_1g01160
malus_domestica MD02G1009600.v1.1
prunus_persica Prupe.7G260400_v2.0.a1
pyrus_communis pycom15g13870
rosa_chinensis RchiOBHm_Chr2g0085951
rosa_laevigata RLG00000015729
rosa_roxburghii Rroxscaffold_2G00154900
rosa_rugosa Rorug01G0464600
rosa_samantha Rh2AG013800 Rh2BG014300 Rh2CG014800 Rh2DG016100
rosa_wichuraiana Rw2G001220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 180
AasI GACNNNNNNGTC 1 cut(s) 196
Acc36I ACCTGC 1 cut(s) 533
AccI GTMKAC 2 cut(s) 93, 207
AciI CCGC 1 cut(s) 372
AfaI GTAC 3 cut(s) 101, 287, 578
AfiI CCNNNNNNNGG 1 cut(s) 272
AflII CTTAAG 1 cut(s) 194
AgsI TTSAA 7 cut(s) 16, 74, 163, 217, 304, 643, 739
AluBI AGCT 8 cut(s) 113, 229, 262, 430, 517, 649, 658, 695
AluI AGCT 8 cut(s) 113, 229, 262, 430, 517, 649, 658, 695
Alw21I GWGCWC 1 cut(s) 170
Alw26I GTCTC 3 cut(s) 239, 690, 765
ApeKI GCWGC 2 cut(s) 262, 517
Asp700I GAANNNNTTC 1 cut(s) 78
AsuC2I CCSGG 1 cut(s) 132
AsuHPI GGTGA 2 cut(s) 487, 680
Bbv12I GWGCWC 1 cut(s) 170
BbvI GCAGC 2 cut(s) 249, 504
BccI CCATC 1 cut(s) 800
BciVI GTATCC 1 cut(s) 112
BclI TGATCA 1 cut(s) 510
BcnI CCSGG 1 cut(s) 132
BcoDI GTCTC 3 cut(s) 239, 690, 765
BfrI CTTAAG 1 cut(s) 194
BfuAI ACCTGC 1 cut(s) 533
BfuI GTATCC 1 cut(s) 112
BisI GCNGC 2 cut(s) 263, 518
BlsI GCNGC 2 cut(s) 264, 519
BmcAI AGTACT 1 cut(s) 287
Bme1390I CCNGG 1 cut(s) 132
BmrFI CCNGG 1 cut(s) 132
BmsI GCATC 3 cut(s) 400, 553, 613
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 1 cut(s) 132
BsaI GGTCTC 2 cut(s) 239, 765
BsaJI CCNNGG 1 cut(s) 150
Bsc4I CCNNNNNNNGG 1 cut(s) 272
Bse1I ACTGG 1 cut(s) 717
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 2 cut(s) 253, 811
BseLI CCNNNNNNNGG 1 cut(s) 272
BseMII CTCAG 2 cut(s) 417, 756
BseNI ACTGG 1 cut(s) 717
BseXI GCAGC 2 cut(s) 249, 504
BseYI CCCAGC 1 cut(s) 229
BsiHKAI GWGCWC 1 cut(s) 170
BsiSI CCGG 1 cut(s) 131
BslFI GGGAC 3 cut(s) 132, 169, 349
BslI CCNNNNNNNGG 1 cut(s) 272
BsmAI GTCTC 3 cut(s) 239, 690, 765
BsmFI GGGAC 3 cut(s) 132, 169, 349
Bso31I GGTCTC 2 cut(s) 239, 765
Bsp1286I GDGCHC 1 cut(s) 170
Bsp143I GATC 2 cut(s) 510, 567
BspACI CCGC 1 cut(s) 372
BspCNI CTCAG 2 cut(s) 418, 757
BspHI TCATGA 1 cut(s) 729
BspMI ACCTGC 1 cut(s) 533
BspTI CTTAAG 1 cut(s) 194
BspTNI GGTCTC 2 cut(s) 239, 765
BsrI ACTGG 1 cut(s) 717
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 2 cut(s) 510, 567
BssT1I CCWWGG 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 1 cut(s) 11
BstAFI CTTAAG 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 651
BstDEI CTNAG 2 cut(s) 426, 765
BstF5I GGATG 2 cut(s) 253, 811
BstKTI GATC 2 cut(s) 513, 570
BstMAI GTCTC 3 cut(s) 239, 690, 765
BstMBI GATC 2 cut(s) 510, 567
BstMWI GCNNNNNNNGC 1 cut(s) 655
BstNSI RCATGY 1 cut(s) 685
BstSCI CCNGG 1 cut(s) 130
BstV1I GCAGC 2 cut(s) 249, 504
BsuI GTATCC 1 cut(s) 112
BtsCI GGATG 2 cut(s) 253, 811
BtsIMutI CAGTG 1 cut(s) 423
BveI ACCTGC 1 cut(s) 533
Cac8I GCNNGC 1 cut(s) 651
CciI TCATGA 1 cut(s) 729
CseI GACGC 1 cut(s) 134
Csp6I GTAC 3 cut(s) 100, 286, 577
CviAII CATG 3 cut(s) 632, 682, 730
CviQI GTAC 3 cut(s) 100, 286, 577
DdeI CTNAG 2 cut(s) 426, 765
DpnI GATC 2 cut(s) 512, 569
DpnII GATC 2 cut(s) 510, 567
DrdI GACNNNNNNGTC 1 cut(s) 196
DseDI GACNNNNNNGTC 1 cut(s) 196
Eam1104I CTCTTC 1 cut(s) 11
EarI CTCTTC 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 150
Eco31I GGTCTC 2 cut(s) 239, 765
EcoT14I CCWWGG 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 150
FaeI CATG 3 cut(s) 635, 685, 733
FalI AAGNNNNNCTT 2 cut(s) 734, 766
FaqI GGGAC 3 cut(s) 132, 169, 349
FatI CATG 3 cut(s) 631, 681, 729
FauNDI CATATG 1 cut(s) 493
FbaI TGATCA 1 cut(s) 510
FblI GTMKAC 2 cut(s) 93, 207
Fnu4HI GCNGC 2 cut(s) 263, 518
FokI GGATG 1 cut(s) 260
Fsp4HI GCNGC 2 cut(s) 263, 518
GluI GCNGC 2 cut(s) 263, 518
GsaI CCCAGC 1 cut(s) 233
HapII CCGG 1 cut(s) 131
HgaI GACGC 1 cut(s) 134
Hin1II CATG 3 cut(s) 635, 685, 733
HinfI GANTC 5 cut(s) 23, 79, 223, 347, 542
HpaII CCGG 1 cut(s) 131
HphI GGTGA 2 cut(s) 487, 680
Hpy166II GTNNAC 5 cut(s) 31, 94, 208, 423, 710
Hpy188I TCNGA 5 cut(s) 84, 189, 247, 609, 703
Hpy188III TCNNGA 7 cut(s) 59, 163, 385, 539, 583, 730, 764
Hpy8I GTNNAC 5 cut(s) 31, 94, 208, 423, 710
Hpy99I CGWCG 1 cut(s) 589
HpyAV CCTTC 4 cut(s) 43, 394, 545, 638
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4IV ACGT 1 cut(s) 239
HpyCH4V TGCA 4 cut(s) 312, 491, 528, 621
HpyF10VI GCNNNNNNNGC 1 cut(s) 655
HpyF3I CTNAG 2 cut(s) 426, 765
HpySE526I ACGT 1 cut(s) 239
Hsp92II CATG 3 cut(s) 635, 685, 733
Ksp22I TGATCA 1 cut(s) 510
Kzo9I GATC 2 cut(s) 510, 567
Lsp1109I GCAGC 2 cut(s) 249, 504
LweI GCATC 3 cut(s) 400, 553, 613
MaeII ACGT 1 cut(s) 239
MaeIII GTNAC 1 cut(s) 475
MalI GATC 2 cut(s) 512, 569
MboI GATC 2 cut(s) 510, 567
MboII GAAGA 2 cut(s) 28, 400
MhlI GDGCHC 1 cut(s) 170
MluCI AATT 5 cut(s) 74, 181, 304, 571, 734
MlyI GAGTC 1 cut(s) 551
MnlI CCTC 4 cut(s) 322, 451, 606, 796
MroXI GAANNNNTTC 1 cut(s) 78
MseI TTAA 4 cut(s) 195, 318, 324, 600
MspA1I CMGCKG 2 cut(s) 229, 372
MspCI CTTAAG 1 cut(s) 194
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 132
MwoI GCNNNNNNNGC 1 cut(s) 655
NciI CCSGG 1 cut(s) 132
NdeI CATATG 1 cut(s) 493
NdeII GATC 2 cut(s) 510, 567
NlaIII CATG 3 cut(s) 635, 685, 733
NmuCI GTSAC 1 cut(s) 475
NspI RCATGY 1 cut(s) 685
PagI TCATGA 1 cut(s) 729
PdmI GAANNNNTTC 1 cut(s) 78
PfeI GAWTC 4 cut(s) 23, 79, 223, 347
PkrI GCNGC 2 cut(s) 264, 519
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
PsiI TTATAA 1 cut(s) 180
PspFI CCCAGC 1 cut(s) 229
PvuII CAGCTG 1 cut(s) 229
RsaI GTAC 3 cut(s) 101, 287, 578
RsaNI GTAC 3 cut(s) 100, 286, 577
SaqAI TTAA 4 cut(s) 195, 318, 324, 600
SatI GCNGC 2 cut(s) 263, 518
Sau3AI GATC 2 cut(s) 510, 567
ScaI AGTACT 1 cut(s) 287
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 1 cut(s) 132
SduI GDGCHC 1 cut(s) 170
SfaNI GCATC 3 cut(s) 400, 553, 613
SmlI CTYRAG 2 cut(s) 194, 353
SmoI CTYRAG 2 cut(s) 194, 353
Sse9I AATT 5 cut(s) 74, 181, 304, 571, 734
SsiI CCGC 1 cut(s) 372
StyD4I CCNGG 1 cut(s) 130
StyI CCWWGG 1 cut(s) 150
TaaI ACNGT 1 cut(s) 290
TaiI ACGT 1 cut(s) 242
TaqI TCGA 1 cut(s) 408
TasI AATT 5 cut(s) 74, 181, 304, 571, 734
TatI WGTACW 2 cut(s) 285, 576
TfiI GAWTC 4 cut(s) 23, 79, 223, 347
Tru1I TTAA 4 cut(s) 195, 318, 324, 600
Tru9I TTAA 4 cut(s) 195, 318, 324, 600
TscAI CASTG 1 cut(s) 430
TseFI GTSAC 1 cut(s) 475
TseI GCWGC 2 cut(s) 262, 517
Tsp45I GTSAC 1 cut(s) 475
TspDTI ATGAA 4 cut(s) 474, 620, 718, 746
TspRI CASTG 1 cut(s) 430
Vha464I CTTAAG 1 cut(s) 194
XceI RCATGY 1 cut(s) 685
XmiI GTMKAC 2 cut(s) 93, 207
XmnI GAANNNNTTC 1 cut(s) 78
ZrmI AGTACT 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.