MD02G1013300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013577
Physical Location & Seq
Forward (+)
846877 .. 849322
2446 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1013300.v1.1.491

Sequence Viewer

Length: 1608 bp
ATGGTATATTCATACGCGCCCGCGTACTACTCCACAATCCACGACTCCATGACCTCCCTGTGCAAGTCCATTCTTCCCTTCAGCTTCAAGAAGCGGCGGTTGCCTGCGGCGGAGCAGAGGCTTTCGAAGCTGCAGTCGGATAACCTCAAGTGGCAGCAGGACTCTTTCCACCAGATTCTGAACTTGATGGGTCTTCACAAAGAAGGCATCTTGGCTGAGACCGAGGTGTCCGCTTTCCGATTGCATTTGCTCGAAACCCTCATTGCCTCTCAGGTGGCGGAGCACGAACAGCCGGTTATTCTGAGAGACAAGTTGCTGTTCTTGCAGGAGCTCTTGTATGCGAAATGCATATCGGAAGACGAGTACCATTCTTCGAAAAGGCCGTTGCTGCAGCGATTAGAGGCACAGGGAGCTGAGATCGAGGCCAAAGATATAATCGCTTCAGGGTCGCTGCCAGCGAAGGATCCGAAAGAGAATACAGATCAAGAGCAGTGGTCGGAAATTGACTTGAAGGATGAGAAATGCTTGCTAACGAAAGATAAAGAGGGTTTGAATTCGAAGAACAAATCGAAACACAGCTCGGCAGTGAAACAAATCAAAGGGGCAGCTTCGGTGTTGGGATTTGTATCACCTTACAAACATGGGAAAAACAGGGGAGAAAAGAGCATATTCGATTTGCCTGATTCGTCAAAATTCTCTTCTTCCAGCTCTTCTAAGCATCAAGCAGGAGATCAATCGTCGATTCTTATGCCGGAAAGCTTGCCACAGGTGCCGGTGAAAGAGAACGGCAGCGCAGATCGAACAAGGAGGAAACCGTTTAGGAGTCTGTTTCAGAGAGAACAGAAAGAGGGTCAGGGGGATCATGCCCACGAAAATGAACAAACACCTTCGAAATCGGCCAAAAAGCAATGGGGTTTTGATGGATTCAAGAAATGGAAGAGGAATGAGTCGGAGGACGAGACAACTCCGCTGCCGCTAAACGAACGATCGGATAGTGAGGCTCACTTGAAGCTTGTTCCAATCCCCATGGGTGAAGGGCCAGACACTAAGCTCATAAAAAGGAGGCTGCTTTCGGATGGTTCTCCTTCCGATTTCTTCATTGACAAGGTTTTAGGGGAGAAGATAAAGAAGGAGCTGTCAAGAATTCAGTCAGAGCTTTGCGCTTCAAATCCAAATCTTCGATTTACGAATGAACAAATTGAAGCTATTTCGACCAGGCTTCCGGTGGACAAGGCTGATCTGAAGAAGTTCTTTCCCAAGTCGTGGTGCGATCGGTATGGAGAAGTTGTTCTGGACGTGGTGAGGAAAGAGTTCAAGGAGCATGTTGGGGAGATGGAGAGCTTGAGAAATGCGGCAAGAGAGAAACACGGTATCCACTCAGCGCGATGGACTGCATTCCAAGATGACGAGGAGAATTGTCACCCGAATCTCTTTGCTCCGCCGCGAGACAACAACCACAATTATACTTCAAAGTTCCATGAAACCAATCCGTTCTGTGATGATCACATTCACACCACCCCTAGGAAGCTGAGATCGAAACCGGCAACTTATACACAAGAGCAGAACCAGAACCAGAATCCCTTTTGGATCCCGGGGCACGGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

536

Amino Acids

61.13

Weight (kDa)

8.01

Isoelectric Point (pI)

51.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013577)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01760 FvH4_1g01760 FvH4_1g01760
malus_domestica MD02G1013300.v1.1 MD15G1160100.v1.1
prunus_persica Prupe.7G255100_v2.0.a1
pyrus_communis pycom02g01240 pycom15g14400
rosa_chinensis RchiOBHm_Chr2g0086721
rosa_laevigata RLG00000015785
rosa_multiflora Rmu_sc0004626.1_g000002
rosa_roxburghii Rroxscaffold_2G00154250
rosa_rugosa Rorug01G0469200
rosa_samantha Rh2AG021200 Rh2BG021100 Rh2CG022000 Rh2DG021800
rosa_wichuraiana Rw2G001720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 226
AccB1I GGYRCC 1 cut(s) 769
AccB7I CCANNNNNTGG 1 cut(s) 1263
AccII CGCG 4 cut(s) 17, 23, 1384, 1444
AclWI GGATC 5 cut(s) 458, 471, 867, 1582, 1595
AcoI YGGCCR 1 cut(s) 897
AcsI RAATTY 3 cut(s) 553, 692, 1143
AcuI CTGAAG 3 cut(s) 64, 426, 1262
AfaI GTAC 2 cut(s) 26, 365
AfiI CCNNNNNNNGG 3 cut(s) 1263, 1521, 1598
AgsI TTSAA 9 cut(s) 88, 511, 553, 928, 1009, 1167, 1202, 1315, 1470
AjiI CACGTC 1 cut(s) 1297
AjnI CCWGG 1 cut(s) 1214
AloI GAACNNNNNNTCC 2 cut(s) 1272, 1304
Alw21I GWGCWC 2 cut(s) 285, 333
Alw26I GTCTC 4 cut(s) 212, 300, 953, 1440
AlwI GGATC 5 cut(s) 458, 471, 867, 1582, 1595
AlwNI CAGNNNCTG 1 cut(s) 178
Ama87I CYCGRG 1 cut(s) 1591
AoxI GGCC 4 cut(s) 380, 423, 897, 1037
ApeKI GCWGC 9 cut(s) 130, 154, 388, 391, 451, 605, 789, 970, 1066
ApoI RAATTY 3 cut(s) 553, 692, 1143
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
Asp700I GAANNNNTTC 3 cut(s) 1247, 1287, 1310
AspA2I CCTAGG 1 cut(s) 1520
AspLEI GCGC 4 cut(s) 19, 794, 1163, 1384
AspS9I GGNCC 1 cut(s) 1037
AsuC2I CCSGG 2 cut(s) 1592, 1593
AsuHPI GGTGA 5 cut(s) 621, 787, 1043, 1312, 1412
AsuII TTCGAA 4 cut(s) 125, 374, 557, 890
AvaI CYCGRG 1 cut(s) 1591
AvrII CCTAGG 1 cut(s) 1520
BaeGI GKGCMC 1 cut(s) 1599
BamHI GGATCC 2 cut(s) 463, 1587
BanI GGYRCC 1 cut(s) 769
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 2 cut(s) 185, 363
Bbv12I GWGCWC 2 cut(s) 285, 333
BbvI GCAGC 9 cut(s) 117, 166, 375, 403, 438, 617, 801, 957, 1053
BccI CCATC 5 cut(s) 181, 914, 1070, 1327, 1380
BceAI ACGGC 2 cut(s) 367, 802
BcgI CGANNNNNNTGC 2 cut(s) 730, 764
BciT130I CCWGG 1 cut(s) 1216
BciVI GTATCC 1 cut(s) 1382
BclI TGATCA 1 cut(s) 1501
BcnI CCSGG 2 cut(s) 1592, 1593
BcoDI GTCTC 4 cut(s) 212, 300, 953, 1440
BfaI CTAG 1 cut(s) 1521
BfmI CTRYAG 2 cut(s) 131, 389
BfuI GTATCC 1 cut(s) 1382
BlnI CCTAGG 1 cut(s) 1520
Bme1390I CCNGG 3 cut(s) 1216, 1592, 1593
BmeT110I CYCGRG 1 cut(s) 1591
BmgBI CACGTC 1 cut(s) 1297
BmgT120I GGNCC 1 cut(s) 1037
BmiI GGNNCC 3 cut(s) 465, 771, 1589
BmrFI CCNGG 3 cut(s) 1216, 1592, 1593
BmsI GCATC 2 cut(s) 216, 727
BpiI GAAGAC 2 cut(s) 185, 363
Bpu14I TTCGAA 4 cut(s) 125, 374, 557, 890
BpuEI CTTGAG 2 cut(s) 131, 1363
BpuMI CCSGG 2 cut(s) 1592, 1593
BsaBI GATNNNNATC 1 cut(s) 625
BsaI GGTCTC 1 cut(s) 212
BsaJI CCNNGG 5 cut(s) 222, 1026, 1520, 1591, 1592
BsaWI WCCGGW 1 cut(s) 1222
BsaXI ACNNNNNCTCC 4 cut(s) 320, 350, 1272, 1302
Bsc4I CCNNNNNNNGG 3 cut(s) 1263, 1521, 1598
Bse118I RCCGGY 3 cut(s) 292, 772, 1540
Bse3DI GCAATG 2 cut(s) 261, 914
Bse8I GATNNNNATC 1 cut(s) 625
BseBI CCWGG 1 cut(s) 1216
BseDI CCNNGG 5 cut(s) 222, 1026, 1520, 1591, 1592
BseGI GGATG 2 cut(s) 520, 1081
BseJI GATNNNNATC 1 cut(s) 625
BseLI CCNNNNNNNGG 3 cut(s) 1263, 1521, 1598
BseMI GCAATG 2 cut(s) 261, 914
BseMII CTCAG 6 cut(s) 207, 284, 293, 405, 1392, 1520
BseRI GAGGAG 1 cut(s) 1424
BseSI GKGCMC 1 cut(s) 1599
BseXI GCAGC 9 cut(s) 117, 166, 375, 403, 438, 617, 801, 957, 1053
Bsh1236I CGCG 4 cut(s) 17, 23, 1384, 1444
Bsh1285I CGRYCG 2 cut(s) 989, 1273
BshFI GGCC 4 cut(s) 382, 425, 899, 1039
BshNI GGYRCC 1 cut(s) 769
BsiEI CGRYCG 2 cut(s) 989, 1273
BsiHKAI GWGCWC 2 cut(s) 285, 333
BsiHKCI CYCGRG 1 cut(s) 1591
BsiSI CCGG 6 cut(s) 293, 752, 773, 1223, 1541, 1592
BslI CCNNNNNNNGG 3 cut(s) 1263, 1521, 1598
BsmAI GTCTC 4 cut(s) 212, 300, 953, 1440
BsmI GAATGC 1 cut(s) 1394
BsnI GGCC 4 cut(s) 382, 425, 899, 1039
Bso31I GGTCTC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 1591
Bsp119I TTCGAA 4 cut(s) 125, 374, 557, 890
Bsp1286I GDGCHC 3 cut(s) 285, 333, 1599
Bsp19I CCATGG 1 cut(s) 1026
BspANI GGCC 4 cut(s) 382, 425, 899, 1039
BspCNI CTCAG 6 cut(s) 208, 283, 294, 406, 1391, 1521
BspFNI CGCG 4 cut(s) 17, 23, 1384, 1444
BspLI GGNNCC 3 cut(s) 465, 771, 1589
BspMAI CTGCAG 2 cut(s) 135, 393
BspPI GGATC 5 cut(s) 458, 471, 867, 1582, 1595
BspQI GCTCTTC 1 cut(s) 715
BspT104I TTCGAA 4 cut(s) 125, 374, 557, 890
BspT107I GGYRCC 1 cut(s) 769
BspTNI GGTCTC 1 cut(s) 212
BsrDI GCAATG 2 cut(s) 261, 914
BsrFI RCCGGY 3 cut(s) 292, 772, 1540
BssAI RCCGGY 3 cut(s) 292, 772, 1540
BssECI CCNNGG 5 cut(s) 222, 1026, 1520, 1591, 1592
BssT1I CCWWGG 2 cut(s) 1026, 1520
Bst2UI CCWGG 1 cut(s) 1216
Bst4CI ACNGT 2 cut(s) 816, 1370
Bst6I CTCTTC 3 cut(s) 703, 715, 932
BstBI TTCGAA 4 cut(s) 125, 374, 557, 890
BstC8I GCNNGC 5 cut(s) 21, 105, 456, 527, 761
BstDEI CTNAG 8 cut(s) 216, 270, 302, 414, 714, 1047, 1378, 1529
BstDSI CCRYGG 1 cut(s) 1026
BstF5I GGATG 2 cut(s) 520, 1081
BstFNI CGCG 4 cut(s) 17, 23, 1384, 1444
BstHHI GCGC 4 cut(s) 19, 794, 1163, 1384
BstMAI GTCTC 4 cut(s) 212, 300, 953, 1440
BstMCI CGRYCG 2 cut(s) 989, 1273
BstMWI GCNNNNNNNGC 7 cut(s) 100, 127, 289, 322, 388, 410, 769
BstNI CCWGG 1 cut(s) 1216
BstNSI RCATGY 1 cut(s) 1325
BstSCI CCNGG 3 cut(s) 1214, 1590, 1591
BstSFI CTRYAG 2 cut(s) 131, 389
BstSLI GKGCMC 1 cut(s) 1599
BstUI CGCG 4 cut(s) 17, 23, 1384, 1444
BstV1I GCAGC 9 cut(s) 117, 166, 375, 403, 438, 617, 801, 957, 1053
BstV2I GAAGAC 2 cut(s) 185, 363
BstX2I RGATCY 2 cut(s) 463, 1587
BstYI RGATCY 2 cut(s) 463, 1587
BsuI GTATCC 1 cut(s) 1382
BsuRI GGCC 4 cut(s) 382, 425, 899, 1039
BtgI CCRYGG 1 cut(s) 1026
BtgZI GCGATG 1 cut(s) 1399
BtrI CACGTC 1 cut(s) 1297
BtsCI GGATG 2 cut(s) 520, 1081
BtsI GCAGTG 2 cut(s) 497, 591
BtsIMutI CAGTG 2 cut(s) 497, 591
Cac8I GCNNGC 5 cut(s) 21, 105, 456, 527, 761
CaiI CAGNNNCTG 1 cut(s) 178
CfoI GCGC 4 cut(s) 19, 794, 1163, 1384
Cfr10I RCCGGY 3 cut(s) 292, 772, 1540
Cfr13I GGNCC 1 cut(s) 1037
Cfr9I CCCGGG 1 cut(s) 1591
Csp6I GTAC 2 cut(s) 25, 364
CspCI CAANNNNNGTGG 2 cut(s) 473, 508
CviAII CATG 6 cut(s) 49, 641, 863, 1027, 1322, 1478
CviQI GTAC 2 cut(s) 25, 364
DdeI CTNAG 8 cut(s) 216, 270, 302, 414, 714, 1047, 1378, 1529
DrdI GACNNNNNNGTC 1 cut(s) 226
DseDI GACNNNNNNGTC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 897
Eam1104I CTCTTC 3 cut(s) 703, 715, 932
EarI CTCTTC 3 cut(s) 703, 715, 932
EciI GGCGGA 3 cut(s) 125, 293, 1428
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 2 cut(s) 1026, 1520
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 212
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 3 cut(s) 64, 426, 1262
Eco88I CYCGRG 1 cut(s) 1591
EcoICRI GAGCTC 1 cut(s) 331
EcoRI GAATTC 2 cut(s) 553, 1143
EcoRII CCWGG 1 cut(s) 1214
EcoT14I CCWWGG 2 cut(s) 1026, 1520
EcoT22I ATGCAT 1 cut(s) 350
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 2 cut(s) 1026, 1520
FaeI CATG 6 cut(s) 52, 644, 866, 1030, 1325, 1481
FalI AAGNNNNNCTT 2 cut(s) 1235, 1267
FatI CATG 6 cut(s) 48, 640, 862, 1026, 1321, 1477
FauI CCCGC 1 cut(s) 28
FbaI TGATCA 1 cut(s) 1501
FokI GGATG 2 cut(s) 527, 1088
FriOI GRGCYC 1 cut(s) 333
FspBI CTAG 1 cut(s) 1521
GlaI GCGC 4 cut(s) 18, 793, 1162, 1383
HaeIII GGCC 4 cut(s) 382, 425, 899, 1039
HapII CCGG 6 cut(s) 293, 752, 773, 1223, 1541, 1592
HhaI GCGC 4 cut(s) 19, 794, 1163, 1384
Hin1II CATG 6 cut(s) 52, 644, 866, 1030, 1325, 1481
Hin6I GCGC 4 cut(s) 17, 792, 1161, 1382
HinP1I GCGC 4 cut(s) 17, 792, 1161, 1382
HindIII AAGCTT 2 cut(s) 757, 1010
HpaII CCGG 6 cut(s) 293, 752, 773, 1223, 1541, 1592
HphI GGTGA 5 cut(s) 621, 787, 1043, 1312, 1412
Hpy166II GTNNAC 1 cut(s) 1228
Hpy188III TCNNGA 5 cut(s) 88, 485, 928, 1140, 1292
Hpy8I GTNNAC 1 cut(s) 1228
Hpy99I CGWCG 1 cut(s) 742
HpyAV CCTTC 8 cut(s) 88, 197, 454, 505, 897, 1028, 1095, 1123
HpyCH4III ACNGT 2 cut(s) 816, 1370
HpyCH4IV ACGT 1 cut(s) 1296
HpyCH4V TGCA 7 cut(s) 63, 133, 244, 325, 348, 391, 1394
HpyF10VI GCNNNNNNNGC 7 cut(s) 100, 127, 289, 322, 388, 410, 769
HpyF3I CTNAG 8 cut(s) 216, 270, 302, 414, 714, 1047, 1378, 1529
HpySE526I ACGT 1 cut(s) 1296
Hsp92II CATG 6 cut(s) 52, 644, 866, 1030, 1325, 1481
HspAI GCGC 4 cut(s) 17, 792, 1161, 1382
Ksp22I TGATCA 1 cut(s) 1501
LguI GCTCTTC 1 cut(s) 715
LmnI GCTCC 7 cut(s) 112, 280, 328, 410, 1132, 1318, 1441
Lsp1109I GCAGC 9 cut(s) 117, 166, 375, 403, 438, 617, 801, 957, 1053
LweI GCATC 2 cut(s) 216, 727
MaeI CTAG 1 cut(s) 1521
MaeII ACGT 1 cut(s) 1296
MaeIII GTNAC 1 cut(s) 1418
MflI RGATCY 2 cut(s) 463, 1587
MhlI GDGCHC 3 cut(s) 285, 333, 1599
MluCI AATT 7 cut(s) 501, 553, 692, 1143, 1197, 1414, 1459
MlyI GAGTC 4 cut(s) 38, 155, 832, 956
MmeI TCCRAC 3 cut(s) 117, 477, 930
Mph1103I ATGCAT 1 cut(s) 350
MroXI GAANNNNTTC 3 cut(s) 1247, 1287, 1310
MslI CAYNNNNRTG 1 cut(s) 873
MspA1I CMGCKG 1 cut(s) 970
MspI CCGG 6 cut(s) 293, 752, 773, 1223, 1541, 1592
MspR9I CCNGG 3 cut(s) 1216, 1592, 1593
Mva1269I GAATGC 1 cut(s) 1394
MvaI CCWGG 1 cut(s) 1216
MvnI CGCG 4 cut(s) 17, 23, 1384, 1444
MwoI GCNNNNNNNGC 7 cut(s) 100, 127, 289, 322, 388, 410, 769
NciI CCSGG 2 cut(s) 1592, 1593
NcoI CCATGG 1 cut(s) 1026
NlaIII CATG 6 cut(s) 52, 644, 866, 1030, 1325, 1481
NlaIV GGNNCC 3 cut(s) 465, 771, 1589
NmeAIII GCCGAG 1 cut(s) 560
NmuCI GTSAC 1 cut(s) 1418
NsiI ATGCAT 1 cut(s) 350
NspI RCATGY 1 cut(s) 1325
NspV TTCGAA 4 cut(s) 125, 374, 557, 890
PciSI GCTCTTC 1 cut(s) 715
PcsI WCGNNNNNNNCGW 2 cut(s) 380, 455
PctI GAATGC 1 cut(s) 1394
PdmI GAANNNNTTC 3 cut(s) 1247, 1287, 1310
PfeI GAWTC 6 cut(s) 175, 683, 742, 924, 1426, 1576
PflMI CCANNNNNTGG 1 cut(s) 1263
Ple19I CGATCG 2 cut(s) 989, 1273
PleI GAGTC 4 cut(s) 38, 155, 831, 955
PpsI GAGTC 4 cut(s) 38, 155, 831, 955
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 1214
PspGI CCWGG 1 cut(s) 1214
PspN4I GGNNCC 3 cut(s) 465, 771, 1589
PspPI GGNCC 1 cut(s) 1037
PstI CTGCAG 2 cut(s) 135, 393
PstNI CAGNNNCTG 1 cut(s) 178
PsuI RGATCY 2 cut(s) 463, 1587
PvuI CGATCG 2 cut(s) 989, 1273
RsaI GTAC 2 cut(s) 26, 365
RsaNI GTAC 2 cut(s) 25, 364
RseI CAYNNNNRTG 1 cut(s) 873
SacI GAGCTC 1 cut(s) 333
SapI GCTCTTC 1 cut(s) 715
Sau96I GGNCC 1 cut(s) 1037
SchI GAGTC 4 cut(s) 38, 155, 832, 956
ScrFI CCNGG 3 cut(s) 1216, 1592, 1593
SduI GDGCHC 3 cut(s) 285, 333, 1599
SfaNI GCATC 2 cut(s) 216, 727
SfcI CTRYAG 2 cut(s) 131, 389
SfuI TTCGAA 4 cut(s) 125, 374, 557, 890
SmaI CCCGGG 1 cut(s) 1593
SmiMI CAYNNNNRTG 1 cut(s) 873
SmlI CTYRAG 2 cut(s) 146, 1342
SmoI CTYRAG 2 cut(s) 146, 1342
Sse9I AATT 7 cut(s) 501, 553, 692, 1143, 1197, 1414, 1459
SspMI CTAG 1 cut(s) 1521
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 3 cut(s) 1214, 1590, 1591
StyI CCWWGG 2 cut(s) 1026, 1520
TaaI ACNGT 2 cut(s) 816, 1370
TaiI ACGT 1 cut(s) 1299
TaqII GACCGA 1 cut(s) 236
TasI AATT 7 cut(s) 501, 553, 692, 1143, 1197, 1414, 1459
TauI GCSGC 5 cut(s) 97, 110, 976, 1355, 1444
TfiI GAWTC 6 cut(s) 175, 683, 742, 924, 1426, 1576
TscAI CASTG 2 cut(s) 497, 591
TseFI GTSAC 1 cut(s) 1418
TseI GCWGC 9 cut(s) 130, 154, 388, 391, 451, 605, 789, 970, 1066
Tsp45I GTSAC 1 cut(s) 1418
TspDTI ATGAA 4 cut(s) 891, 1087, 1206, 1494
TspGWI ACGGA 1 cut(s) 1479
TspMI CCCGGG 1 cut(s) 1591
TspRI CASTG 2 cut(s) 497, 591
Van91I CCANNNNNTGG 1 cut(s) 1263
XapI RAATTY 3 cut(s) 553, 692, 1143
XceI RCATGY 1 cut(s) 1325
XcmI CCANNNNNNNNNTGG 1 cut(s) 1222
XmaI CCCGGG 1 cut(s) 1591
XmaJI CCTAGG 1 cut(s) 1520
XmnI GAANNNNTTC 3 cut(s) 1247, 1287, 1310
XspI CTAG 1 cut(s) 1521
Zsp2I ATGCAT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.