Rroxscaffold_2G00154250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013577
Physical Location & Seq
Reverse (-)
90903181 .. 90905494
2314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00154250.1

Sequence Viewer

Length: 1575 bp
ATGGTGTACTCATACGCACCCACATATTACTCCTCTCTACATGAGTCCGTCACCTCTTTATGCAAGACCATCCTTCCCTTCAGCTTCAAGAAACGCCGCTTACCGGCCGCGGAACACAAGCTGTCGAAGCTTCAGTCTGATAATCTCAAATGGCAGCAGGACTCTTTCCACCAGATTCTCAACTTGATGGGTCTTCACAAGGAAGGCATTTTGGCCGAGACTGAGGTCTCAGCTTTTCGATCCCATTTGCTCGACACTCTAATTGCAGCTCCGGCCGAACATGAGCAGCCTGTGATTTTGAGAGATAAGCTGCTTTTCTTGCAGGAACTTTTGTATGCAAAATGCATATCTGAAGATGAGTACCATTCTTCGAAAAGGCCGTTGCTGCAGAGATTGGCGGTCCAAGGAGCTGAGATTGAGGCCAGAGATGTAATTGTTGCAGGGGCACCACTCAAAGACCCAAAAGAGAATGCAGAGGAGGAATGGTCTGTCATCGACTTGAAGGATGACAAAAGCTTGGTGAACAAAGAGGGCTCGAATTCGAAGAGCAACAAAGGGAGGCATGGCTCGGCCATGAAGCAGATCAAAGGAGCAGCTTCGGTTTTGGGTTTTGTGTCACCTTACAAACAGGGGAAGAACAGAGAAGAGAGGAGCATTTTTGATTTGGCTAACAACTCTAATCATGAAACAAGTTCAATTCTCATGCCGGAAAGCTTGCCTCCAGTGAAAGAAAGTGGTAGCTCGGATATGAAGAAGAGGAAACCGTTTAGGAATCTGTTTCAGAGAGAGCAGAAAGAGGGAAATGGGGATGAGAATTGTGTCGAACAAGACCAAACACCTTCGAAATCAGCGAAAAAGAAATGGGGATTTGATGGATTGAAGAAGTGGAAGAGGAGTGAGTTGGAGGATGAGACAACTCCTCTGCCTCTTAATGAGAGATCAGATAGTGAAGTCCATGTGAAGCTTGTGGCAAGTCCCATGGGTGAAGGACCAGACACTAAGCTTATCAAGAGGAAACTGCTTTCAGATGGTTCTCCTTCTGATTTCTTCATTGATAAGGTTGTAGGGGACAAGATAAAGAAGGAGCTGTCGAGAATTCAGACCGAGCTTTGCACATCAAATCCAAATCTCAAATTTTCGAATGACCAAATTGAAGCAATTTCGACTAAGCTTCCAGTAGACAAGGCTGAGCTAAAGAAGTTTTTCCCCAAGTCGTGGTGTGATCGCTACGGTGATGTGGTCTTGGATGTGGTGAAGAAAGAATTCAAGGACCATGTTGGGGAGATGGAGAGCTTGAGGAATGCAGCTAGAGAGAAACATGGCATGAACTCAGCACGGTGGACTACATTCCAAGATGACGATGAGAATTGTCACCCGAATCTCTTTGCAACACGAGATCATTCATACCATATCAGTGGCAATTCAAAGTTCCATGAAACCAATCCTTTCTGTGATGATCACATCACTGGGAATGGAAGCAATGCTAGCAAGCTGGGAATTAAACCAGCTTTTGCCCAAGATCAGAACCAGAACCAGAACCAGAACCCCTTCTGGGTCCCTGGACATGGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

524

Amino Acids

59.27

Weight (kDa)

7.02

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013577)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01760 FvH4_1g01760 FvH4_1g01760
malus_domestica MD02G1013300.v1.1 MD15G1160100.v1.1
prunus_persica Prupe.7G255100_v2.0.a1
pyrus_communis pycom02g01240 pycom15g14400
rosa_chinensis RchiOBHm_Chr2g0086721
rosa_laevigata RLG00000015785
rosa_multiflora Rmu_sc0004626.1_g000002
rosa_roxburghii Rroxscaffold_2G00154250
rosa_rugosa Rorug01G0469200
rosa_samantha Rh2AG021200 Rh2BG021100 Rh2CG022000 Rh2DG021800
rosa_wichuraiana Rw2G001720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 445
AccB7I CCANNNNNTGG 1 cut(s) 1215
AccI GTMKAC 1 cut(s) 1179
AccII CGCG 1 cut(s) 110
AciI CCGC 4 cut(s) 97, 108, 110, 398
AclWI GGATC 1 cut(s) 234
AcoI YGGCCR 4 cut(s) 105, 213, 273, 570
AcsI RAATTY 4 cut(s) 538, 1095, 1133, 1262
AcuI CTGAAG 3 cut(s) 64, 116, 372
AfaI GTAC 2 cut(s) 8, 362
AfiI CCNNNNNNNGG 5 cut(s) 103, 1215, 1279, 1552, 1565
AgsI TTSAA 7 cut(s) 88, 502, 696, 880, 1154, 1267, 1425
AjnI CCWGG 1 cut(s) 1558
Alw26I GTCTC 3 cut(s) 212, 232, 905
AlwI GGATC 1 cut(s) 234
AoxI GGCC 6 cut(s) 105, 213, 273, 377, 420, 570
ApeKI GCWGC 7 cut(s) 154, 266, 286, 310, 385, 593, 1304
ApoI RAATTY 4 cut(s) 538, 1095, 1133, 1262
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
Asp700I GAANNNNTTC 3 cut(s) 1202, 1262, 1547
AspS9I GGNCC 4 cut(s) 400, 989, 1270, 1555
AsuHPI GGTGA 7 cut(s) 43, 532, 609, 995, 1244, 1264, 1364
AsuII TTCGAA 4 cut(s) 371, 542, 842, 1139
AsuNHI GCTAGC 1 cut(s) 1484
AvaII GGWCC 4 cut(s) 400, 989, 1270, 1555
BaeGI GKGCMC 1 cut(s) 448
BanI GGYRCC 1 cut(s) 445
BanII GRGCYC 1 cut(s) 536
BauI CACGAG 1 cut(s) 1392
BbsI GAAGAC 1 cut(s) 185
BbvI GCAGC 7 cut(s) 166, 278, 297, 298, 372, 605, 1316
BccI CCATC 5 cut(s) 77, 181, 866, 1022, 1279
BceAI ACGGC 1 cut(s) 364
BciT130I CCWGG 1 cut(s) 1560
BclI TGATCA 1 cut(s) 1456
BcoDI GTCTC 3 cut(s) 212, 232, 905
BfaI CTAG 2 cut(s) 1308, 1485
BfmI CTRYAG 1 cut(s) 386
BisI GCNGC 9 cut(s) 97, 108, 155, 267, 287, 311, 386, 594, 1305
BlpI GCTNAGC 1 cut(s) 1188
BlsI GCNGC 9 cut(s) 98, 109, 156, 268, 288, 312, 387, 595, 1306
Bme1390I CCNGG 1 cut(s) 1560
Bme18I GGWCC 4 cut(s) 400, 989, 1270, 1555
BmgT120I GGNCC 4 cut(s) 400, 989, 1270, 1555
BmiI GGNNCC 3 cut(s) 447, 1556, 1557
BmrFI CCNGG 1 cut(s) 1560
BmrI ACTGGG 1 cut(s) 1476
BmtI GCTAGC 1 cut(s) 1488
BmuI ACTGGG 1 cut(s) 1476
BoxI GACNNNNGTC 1 cut(s) 224
BpiI GAAGAC 1 cut(s) 185
BpmI CTGGAG 1 cut(s) 705
Bpu1102I GCTNAGC 1 cut(s) 1188
Bpu14I TTCGAA 4 cut(s) 371, 542, 842, 1139
BpuEI CTTGAG 1 cut(s) 1315
BsaI GGTCTC 1 cut(s) 232
BsaJI CCNNGG 4 cut(s) 108, 403, 978, 1558
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 5 cut(s) 103, 1215, 1279, 1552, 1565
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 3 cut(s) 722, 1175, 1471
Bse3DI GCAATG 1 cut(s) 1486
BseBI CCWGG 1 cut(s) 1560
BseDI CCNNGG 4 cut(s) 108, 403, 978, 1558
BseGI GGATG 5 cut(s) 69, 511, 814, 913, 1252
BseLI CCNNNNNNNGG 5 cut(s) 103, 1215, 1279, 1552, 1565
BseMI GCAATG 1 cut(s) 1486
BseMII CTCAG 5 cut(s) 213, 243, 402, 1179, 1344
BseNI ACTGG 3 cut(s) 722, 1175, 1471
BseRI GAGGAG 5 cut(s) 22, 491, 664, 907, 909
BseSI GKGCMC 1 cut(s) 448
BseX3I CGGCCG 2 cut(s) 105, 273
BseXI GCAGC 7 cut(s) 166, 278, 297, 298, 372, 605, 1316
BseYI CCCAGC 1 cut(s) 1492
Bsh1236I CGCG 1 cut(s) 110
Bsh1285I CGRYCG 2 cut(s) 108, 276
BshFI GGCC 6 cut(s) 107, 215, 275, 379, 422, 572
BshNI GGYRCC 1 cut(s) 445
BsiEI CGRYCG 2 cut(s) 108, 276
BsiSI CCGG 3 cut(s) 104, 272, 707
BslFI GGGAC 3 cut(s) 960, 1082, 1541
BslI CCNNNNNNNGG 5 cut(s) 103, 1215, 1279, 1552, 1565
BsmAI GTCTC 3 cut(s) 212, 232, 905
BsmFI GGGAC 3 cut(s) 960, 1082, 1541
BsmI GAATGC 2 cut(s) 475, 1306
BsnI GGCC 6 cut(s) 107, 215, 275, 379, 422, 572
Bso31I GGTCTC 1 cut(s) 232
Bsp119I TTCGAA 4 cut(s) 371, 542, 842, 1139
Bsp1286I GDGCHC 2 cut(s) 448, 536
Bsp143I GATC 7 cut(s) 239, 582, 938, 1222, 1396, 1456, 1519
Bsp1720I GCTNAGC 1 cut(s) 1188
Bsp19I CCATGG 1 cut(s) 978
BspACI CCGC 4 cut(s) 97, 108, 110, 398
BspANI GGCC 6 cut(s) 107, 215, 275, 379, 422, 572
BspCNI CTCAG 5 cut(s) 214, 242, 403, 1180, 1343
BspFNI CGCG 1 cut(s) 110
BspHI TCATGA 1 cut(s) 682
BspLI GGNNCC 3 cut(s) 447, 1556, 1557
BspMAI CTGCAG 1 cut(s) 390
BspOI GCTAGC 1 cut(s) 1488
BspPI GGATC 1 cut(s) 234
BspQI GCTCTTC 1 cut(s) 539
BspT104I TTCGAA 4 cut(s) 371, 542, 842, 1139
BspT107I GGYRCC 1 cut(s) 445
BspTNI GGTCTC 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 1486
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 3 cut(s) 722, 1175, 1471
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 4 cut(s) 108, 403, 978, 1558
BssMI GATC 7 cut(s) 239, 582, 938, 1222, 1396, 1456, 1519
BssSI CACGAG 1 cut(s) 1392
BssT1I CCWWGG 2 cut(s) 403, 978
Bst2BI CACGAG 1 cut(s) 1392
Bst2UI CCWGG 1 cut(s) 1560
Bst4CI ACNGT 3 cut(s) 765, 1232, 1338
Bst6I CTCTTC 4 cut(s) 539, 639, 749, 884
BstBI TTCGAA 4 cut(s) 371, 542, 842, 1139
BstC8I GCNNGC 3 cut(s) 716, 1486, 1490
BstDEI CTNAG 7 cut(s) 222, 229, 411, 999, 1167, 1188, 1330
BstDSI CCRYGG 2 cut(s) 108, 978
BstF5I GGATG 5 cut(s) 69, 511, 814, 913, 1252
BstFNI CGCG 1 cut(s) 110
BstKTI GATC 7 cut(s) 242, 585, 941, 1225, 1399, 1459, 1522
BstMAI GTCTC 3 cut(s) 212, 232, 905
BstMBI GATC 7 cut(s) 239, 582, 938, 1222, 1396, 1456, 1519
BstMCI CGRYCG 2 cut(s) 108, 276
BstMWI GCNNNNNNNGC 5 cut(s) 127, 272, 319, 385, 1485
BstNI CCWGG 1 cut(s) 1560
BstPAI GACNNNNGTC 1 cut(s) 224
BstSCI CCNGG 1 cut(s) 1558
BstSFI CTRYAG 1 cut(s) 386
BstSLI GKGCMC 1 cut(s) 448
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 7 cut(s) 166, 278, 297, 298, 372, 605, 1316
BstV2I GAAGAC 1 cut(s) 185
BstXI CCANNNNNNTGG 1 cut(s) 1415
BstZI CGGCCG 2 cut(s) 105, 273
BsuRI GGCC 6 cut(s) 107, 215, 275, 379, 422, 572
BtgI CCRYGG 2 cut(s) 108, 978
BtsCI GGATG 5 cut(s) 69, 511, 814, 913, 1252
BtsIMutI CAGTG 3 cut(s) 729, 1420, 1464
Cac8I GCNNGC 3 cut(s) 716, 1486, 1490
CciI TCATGA 1 cut(s) 682
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 4 cut(s) 400, 989, 1270, 1555
Cfr42I CCGCGG 1 cut(s) 111
Csp6I GTAC 2 cut(s) 7, 361
CviQI GTAC 2 cut(s) 7, 361
DdeI CTNAG 7 cut(s) 222, 229, 411, 999, 1167, 1188, 1330
DpnI GATC 7 cut(s) 241, 584, 940, 1224, 1398, 1458, 1521
DpnII GATC 7 cut(s) 239, 582, 938, 1222, 1396, 1456, 1519
EaeI YGGCCR 4 cut(s) 105, 213, 273, 570
EagI CGGCCG 2 cut(s) 105, 273
Eam1104I CTCTTC 4 cut(s) 539, 639, 749, 884
EarI CTCTTC 4 cut(s) 539, 639, 749, 884
EclXI CGGCCG 2 cut(s) 105, 273
Eco130I CCWWGG 2 cut(s) 403, 978
Eco24I GRGCYC 1 cut(s) 536
Eco31I GGTCTC 1 cut(s) 232
Eco47I GGWCC 4 cut(s) 400, 989, 1270, 1555
Eco52I CGGCCG 2 cut(s) 105, 273
Eco57I CTGAAG 3 cut(s) 64, 116, 372
EcoO109I RGGNCCY 1 cut(s) 1555
EcoRI GAATTC 3 cut(s) 538, 1095, 1262
EcoRII CCWGG 1 cut(s) 1558
EcoT14I CCWWGG 2 cut(s) 403, 978
EcoT22I ATGCAT 1 cut(s) 347
EcoT38I GRGCYC 1 cut(s) 536
ErhI CCWWGG 2 cut(s) 403, 978
FaqI GGGAC 3 cut(s) 960, 1082, 1541
FbaI TGATCA 1 cut(s) 1456
FblI GTMKAC 1 cut(s) 1179
Fnu4HI GCNGC 9 cut(s) 97, 108, 155, 267, 287, 311, 386, 594, 1305
FokI GGATG 5 cut(s) 56, 518, 821, 920, 1259
FriOI GRGCYC 1 cut(s) 536
Fsp4HI GCNGC 9 cut(s) 97, 108, 155, 267, 287, 311, 386, 594, 1305
FspBI CTAG 2 cut(s) 1308, 1485
GluI GCNGC 9 cut(s) 97, 108, 155, 267, 287, 311, 386, 594, 1305
GsaI CCCAGC 1 cut(s) 1496
GsuI CTGGAG 1 cut(s) 705
HaeIII GGCC 6 cut(s) 107, 215, 275, 379, 422, 572
HapII CCGG 3 cut(s) 104, 272, 707
HindIII AAGCTT 6 cut(s) 128, 514, 712, 962, 1001, 1169
HinfI GANTC 5 cut(s) 44, 161, 175, 772, 1378
HpaII CCGG 3 cut(s) 104, 272, 707
HphI GGTGA 7 cut(s) 43, 532, 609, 995, 1244, 1264, 1364
Hpy166II GTNNAC 4 cut(s) 7, 523, 1180, 1341
Hpy188I TCNGA 9 cut(s) 139, 352, 745, 783, 943, 1027, 1042, 1101, 1524
Hpy188III TCNNGA 4 cut(s) 88, 683, 1009, 1092
Hpy8I GTNNAC 4 cut(s) 7, 523, 1180, 1341
HpyAV CCTTC 9 cut(s) 83, 88, 197, 496, 849, 980, 1047, 1075, 1558
HpyCH4III ACNGT 3 cut(s) 765, 1232, 1338
HpyF10VI GCNNNNNNNGC 5 cut(s) 127, 272, 319, 385, 1485
HpyF3I CTNAG 7 cut(s) 222, 229, 411, 999, 1167, 1188, 1330
KflI GGGWCCC 1 cut(s) 1555
Ksp22I TGATCA 1 cut(s) 1456
KspI CCGCGG 1 cut(s) 111
Kzo9I GATC 7 cut(s) 239, 582, 938, 1222, 1396, 1456, 1519
LguI GCTCTTC 1 cut(s) 539
LmnI GCTCC 5 cut(s) 274, 407, 590, 651, 1084
Lsp1109I GCAGC 7 cut(s) 166, 278, 297, 298, 372, 605, 1316
MaeI CTAG 2 cut(s) 1308, 1485
MaeIII GTNAC 3 cut(s) 49, 615, 1370
MalI GATC 7 cut(s) 241, 584, 940, 1224, 1398, 1458, 1521
MboI GATC 7 cut(s) 239, 582, 938, 1222, 1396, 1456, 1519
MhlI GDGCHC 2 cut(s) 448, 536
MlyI GAGTC 2 cut(s) 53, 155
MmeI TCCRAC 1 cut(s) 882
Mph1103I ATGCAT 1 cut(s) 347
MroXI GAANNNNTTC 3 cut(s) 1202, 1262, 1547
MseI TTAA 2 cut(s) 930, 1500
MslI CAYNNNNRTG 1 cut(s) 1413
MspA1I CMGCKG 1 cut(s) 110
MspI CCGG 3 cut(s) 104, 272, 707
MspR9I CCNGG 1 cut(s) 1560
Mva1269I GAATGC 2 cut(s) 475, 1306
MvaI CCWGG 1 cut(s) 1560
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 5 cut(s) 127, 272, 319, 385, 1485
NcoI CCATGG 1 cut(s) 978
NdeII GATC 7 cut(s) 239, 582, 938, 1222, 1396, 1456, 1519
NheI GCTAGC 1 cut(s) 1484
NlaIV GGNNCC 3 cut(s) 447, 1556, 1557
NmeAIII GCCGAG 2 cut(s) 241, 548
NmuCI GTSAC 3 cut(s) 49, 615, 1370
NsiI ATGCAT 1 cut(s) 347
NspV TTCGAA 4 cut(s) 371, 542, 842, 1139
PagI TCATGA 1 cut(s) 682
PciSI GCTCTTC 1 cut(s) 539
PcsI WCGNNNNNNNCGW 2 cut(s) 377, 848
PctI GAATGC 2 cut(s) 475, 1306
PdmI GAANNNNTTC 3 cut(s) 1202, 1262, 1547
PfeI GAWTC 3 cut(s) 175, 772, 1378
PflMI CCANNNNNTGG 1 cut(s) 1215
PkrI GCNGC 9 cut(s) 98, 109, 156, 268, 288, 312, 387, 595, 1306
PleI GAGTC 2 cut(s) 52, 155
PpsI GAGTC 2 cut(s) 52, 155
PpuMI RGGWCCY 1 cut(s) 1555
PshAI GACNNNNGTC 1 cut(s) 224
Psp5II RGGWCCY 1 cut(s) 1555
Psp6I CCWGG 1 cut(s) 1558
PspFI CCCAGC 1 cut(s) 1492
PspGI CCWGG 1 cut(s) 1558
PspN4I GGNNCC 3 cut(s) 447, 1556, 1557
PspPI GGNCC 4 cut(s) 400, 989, 1270, 1555
PspPPI RGGWCCY 1 cut(s) 1555
PstI CTGCAG 1 cut(s) 390
RsaI GTAC 2 cut(s) 8, 362
RsaNI GTAC 2 cut(s) 7, 361
RseI CAYNNNNRTG 1 cut(s) 1413
SacII CCGCGG 1 cut(s) 111
SapI GCTCTTC 1 cut(s) 539
SaqAI TTAA 2 cut(s) 930, 1500
SatI GCNGC 9 cut(s) 97, 108, 155, 267, 287, 311, 386, 594, 1305
Sau3AI GATC 7 cut(s) 239, 582, 938, 1222, 1396, 1456, 1519
Sau96I GGNCC 4 cut(s) 400, 989, 1270, 1555
SchI GAGTC 2 cut(s) 53, 155
ScrFI CCNGG 1 cut(s) 1560
SduI GDGCHC 2 cut(s) 448, 536
SfcI CTRYAG 1 cut(s) 386
Sfr303I CCGCGG 1 cut(s) 111
SfuI TTCGAA 4 cut(s) 371, 542, 842, 1139
SgrBI CCGCGG 1 cut(s) 111
SinI GGWCC 4 cut(s) 400, 989, 1270, 1555
SmiMI CAYNNNNRTG 1 cut(s) 1413
SmlI CTYRAG 1 cut(s) 1294
SmoI CTYRAG 1 cut(s) 1294
SsiI CCGC 4 cut(s) 97, 108, 110, 398
SspMI CTAG 2 cut(s) 1308, 1485
StyD4I CCNGG 1 cut(s) 1558
StyI CCWWGG 2 cut(s) 403, 978
TaaI ACNGT 3 cut(s) 765, 1232, 1338
TaqII GACCGA 1 cut(s) 1118
TatI WGTACW 1 cut(s) 6
TauI GCSGC 2 cut(s) 99, 110
TfiI GAWTC 3 cut(s) 175, 772, 1378
Tru1I TTAA 2 cut(s) 930, 1500
Tru9I TTAA 2 cut(s) 930, 1500
TscAI CASTG 3 cut(s) 729, 1420, 1471
TseFI GTSAC 3 cut(s) 49, 615, 1370
TseI GCWGC 7 cut(s) 154, 266, 286, 310, 385, 593, 1304
Tsp45I GTSAC 3 cut(s) 49, 615, 1370
TspDTI ATGAA 7 cut(s) 590, 699, 764, 1039, 1340, 1392, 1449
TspGWI ACGGA 1 cut(s) 37
TspRI CASTG 3 cut(s) 729, 1420, 1471
Van91I CCANNNNNTGG 1 cut(s) 1215
VpaK11BI GGWCC 4 cut(s) 400, 989, 1270, 1555
XapI RAATTY 4 cut(s) 538, 1095, 1133, 1262
XmiI GTMKAC 1 cut(s) 1179
XmnI GAANNNNTTC 3 cut(s) 1202, 1262, 1547
XspI CTAG 2 cut(s) 1308, 1485
Zsp2I ATGCAT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.