Rh2AG021200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013577
Physical Location & Seq
Forward (+)
1533579 .. 1535928
2350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG021200.1

Sequence Viewer

Length: 1563 bp
ATGGTGTACTCTTACGCACCCACATACTACTCCTCTCTACATGAGTCCGTCACCTCTTTATGCAAGACCATCCTTCCCTTCAGCTTCAAGAAACGCCGCTTGCCGGCCGCGGAACACAAGCTGTCGAAGCTTCAGTCTGATAATCTCAAATGGCAGCAGGACTCTTTCCACCAGATTCTCAACTTGATGGGTCTTCACAAAGAAGGCATTTTGGCCGAGACTGAGGTCTCAGCTTTTCGATCCCATTTGCTCGACACTCTAATTGCAGCTCCGGCCGAACATGAGCAGCCAGTGATCTTGAGAGATAAGCTGCTTTTCTTGCAGGAACTTTTGTATGCAAAGTGCATATCTGAAGATGAGTACCATTCTTCGAAAAGGCCGCTGCTGCAGAGATTGGCAGTCCAAGGAGCTGAGATTGAGGCCAGAGATGTAATTGTTGCAGGGGCACCACTTAATGATCCAAAAGAGAATGCAGAGGAGGAGTGGTCTGTGATTGACTTGAAGGATGACAAAAGCTTGATGAACAAAGAGGGTTCGAATTCGAAGAGCAACAAAGGGAAGCATGGCTCGGCACTGAAGCAGATTAAAGGAGCAGCTTCGGTTTTGGGGTTTGTGTCACCTTACAAACAGGGGAAGAACAGAGAAGAGAGGAGCATTTTCGATTTGGCTAACAACTCTAATCATGAAACAAGCTCAATTCTCATGCCAGAGAGCTTGCCTCCTGTGAAAGAAAGTGGTAGCTCAGATAGGACAAAGAGGAAACCGTTTAGGAATTTGTTTCAGAGAGAACAGAAAGAGGGAAATGGGGATGAGAATTGTGTCGAGCAAGAACAAACACCTTCGAAATCAGCGAAAAAGAAATGGGGGTTTGATGGATTGAAGAAGTGGAAGAGGAGTGAGGTGGAGGATGAGACAACTCCTCTGCCTCTTAATGAGAGATCAGATAGTGAAGTTCATGTGAAGCTTGTGGCAAGTCCCGTGGGTGAAGGACCAGACACTAAGCTTATCAAGAGGAAGCTGCTGTCAGACGGTTCTCCTTCTGATTTTTTCATTGATAAGGTTGTAGGGGACAAGATAAAGAAGGAGCTGTCGAGAATTCAGACCGAGCTTTGCACATCAAATCCAAATCTCAAATTTTCGAATGACCAAATTGAAGCAATTTCAACTAAGCTTCCAGTAGACAAGGCTGAGCTAAAGAAGTTTTTCCCCAAGTCGTGGTGTGATCGCTACGGTGATGTGGTCTTGGATGTGGTGAAGAAAGAATTCAAGGACCATGTTGGGGAGATGGAGAGCTTGAGGAATGCAGCTAGAGAGAAACATGGCATGAACTCAGCGCGATGGACAACATTTCAAGACGACGATGAAAATTGTCACCCGAATCTATTTGCAACCCGAGATCATTCATACCATAGCAGTGGCACTTCAAAGTTCCATGAAACCAATCCTTTCTGTGATGATCACATCACTGGGAATGGAAGCAATGCTAGCAAGCTGGGATTTAAACCAGCTTTTGCCCAAGACCAGAACCAGAACCCCTTCTGGGTCCCTGGACATGGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

520

Amino Acids

58.71

Weight (kDa)

6.82

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013577)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01760 FvH4_1g01760 FvH4_1g01760
malus_domestica MD02G1013300.v1.1 MD15G1160100.v1.1
prunus_persica Prupe.7G255100_v2.0.a1
pyrus_communis pycom02g01240 pycom15g14400
rosa_chinensis RchiOBHm_Chr2g0086721
rosa_laevigata RLG00000015785
rosa_multiflora Rmu_sc0004626.1_g000002
rosa_roxburghii Rroxscaffold_2G00154250
rosa_rugosa Rorug01G0469200
rosa_samantha Rh2AG021200 Rh2BG021100 Rh2CG022000 Rh2DG021800
rosa_wichuraiana Rw2G001720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 445
AccB7I CCANNNNNTGG 1 cut(s) 1215
AccI GTMKAC 1 cut(s) 1179
AccII CGCG 2 cut(s) 110, 1336
AciI CCGC 4 cut(s) 97, 108, 110, 380
AclWI GGATC 2 cut(s) 234, 452
AcoI YGGCCR 3 cut(s) 105, 213, 273
AcsI RAATTY 5 cut(s) 538, 772, 1095, 1133, 1262
AcuI CTGAAG 4 cut(s) 64, 116, 372, 596
AfaI GTAC 2 cut(s) 8, 362
AfiI CCNNNNNNNGG 5 cut(s) 103, 1215, 1279, 1540, 1553
AgsI TTSAA 8 cut(s) 88, 502, 880, 1154, 1164, 1267, 1352, 1425
AjnI CCWGG 1 cut(s) 1546
Alw26I GTCTC 3 cut(s) 212, 232, 905
AlwI GGATC 2 cut(s) 234, 452
Ama87I CYCGRG 1 cut(s) 1392
AoxI GGCC 5 cut(s) 105, 213, 273, 377, 420
ApeKI GCWGC 9 cut(s) 154, 266, 286, 310, 382, 385, 593, 1018, 1304
ApoI RAATTY 5 cut(s) 538, 772, 1095, 1133, 1262
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
Asp700I GAANNNNTTC 3 cut(s) 1202, 1262, 1535
AspLEI GCGC 1 cut(s) 1336
AspS9I GGNCC 3 cut(s) 989, 1270, 1543
AsuHPI GGTGA 6 cut(s) 43, 609, 995, 1244, 1264, 1364
AsuII TTCGAA 5 cut(s) 371, 536, 542, 842, 1139
AsuNHI GCTAGC 1 cut(s) 1484
AvaI CYCGRG 1 cut(s) 1392
AvaII GGWCC 3 cut(s) 989, 1270, 1543
BaeGI GKGCMC 1 cut(s) 448
BanI GGYRCC 1 cut(s) 445
BbsI GAAGAC 1 cut(s) 185
BbvI GCAGC 9 cut(s) 166, 278, 297, 298, 369, 372, 605, 1005, 1316
BccI CCATC 5 cut(s) 77, 181, 866, 1279, 1332
BciT130I CCWGG 1 cut(s) 1548
BclI TGATCA 1 cut(s) 1456
BcoDI GTCTC 3 cut(s) 212, 232, 905
BfaI CTAG 2 cut(s) 1308, 1485
BfmI CTRYAG 1 cut(s) 386
BlpI GCTNAGC 1 cut(s) 1188
Bme1390I CCNGG 1 cut(s) 1548
Bme18I GGWCC 3 cut(s) 989, 1270, 1543
BmeT110I CYCGRG 1 cut(s) 1392
BmgT120I GGNCC 3 cut(s) 989, 1270, 1543
BmiI GGNNCC 3 cut(s) 447, 1544, 1545
BmrFI CCNGG 1 cut(s) 1548
BmrI ACTGGG 1 cut(s) 1476
BmtI GCTAGC 1 cut(s) 1488
BmuI ACTGGG 1 cut(s) 1476
BoxI GACNNNNGTC 1 cut(s) 224
BpiI GAAGAC 1 cut(s) 185
BplI GAGNNNNNCTC 2 cut(s) 703, 735
Bpu1102I GCTNAGC 1 cut(s) 1188
Bpu14I TTCGAA 5 cut(s) 371, 536, 542, 842, 1139
BpuEI CTTGAG 2 cut(s) 319, 1315
BsaI GGTCTC 1 cut(s) 232
BsaJI CCNNGG 4 cut(s) 108, 403, 978, 1546
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 5 cut(s) 103, 1215, 1279, 1540, 1553
Bse118I RCCGGY 1 cut(s) 103
Bse1I ACTGG 3 cut(s) 290, 1175, 1471
Bse3DI GCAATG 1 cut(s) 1486
BseBI CCWGG 1 cut(s) 1548
BseDI CCNNGG 4 cut(s) 108, 403, 978, 1546
BseGI GGATG 5 cut(s) 69, 511, 814, 913, 1252
BseLI CCNNNNNNNGG 5 cut(s) 103, 1215, 1279, 1540, 1553
BseMI GCAATG 1 cut(s) 1486
BseMII CTCAG 6 cut(s) 213, 243, 402, 756, 1179, 1344
BseNI ACTGG 3 cut(s) 290, 1175, 1471
BseRI GAGGAG 6 cut(s) 22, 491, 494, 664, 907, 909
BseSI GKGCMC 1 cut(s) 448
BseX3I CGGCCG 2 cut(s) 105, 273
BseXI GCAGC 9 cut(s) 166, 278, 297, 298, 369, 372, 605, 1005, 1316
BseYI CCCAGC 1 cut(s) 1492
Bsh1236I CGCG 2 cut(s) 110, 1336
Bsh1285I CGRYCG 2 cut(s) 108, 276
BshFI GGCC 5 cut(s) 107, 215, 275, 379, 422
BshNI GGYRCC 1 cut(s) 445
BsiEI CGRYCG 2 cut(s) 108, 276
BsiHKCI CYCGRG 1 cut(s) 1392
BsiSI CCGG 2 cut(s) 104, 272
BslFI GGGAC 3 cut(s) 960, 1082, 1529
BslI CCNNNNNNNGG 5 cut(s) 103, 1215, 1279, 1540, 1553
BsmAI GTCTC 3 cut(s) 212, 232, 905
BsmFI GGGAC 3 cut(s) 960, 1082, 1529
BsmI GAATGC 2 cut(s) 475, 1306
BsnI GGCC 5 cut(s) 107, 215, 275, 379, 422
Bso31I GGTCTC 1 cut(s) 232
BsoBI CYCGRG 1 cut(s) 1392
Bsp119I TTCGAA 5 cut(s) 371, 536, 542, 842, 1139
Bsp1286I GDGCHC 1 cut(s) 448
Bsp143I GATC 7 cut(s) 239, 294, 457, 938, 1222, 1396, 1456
Bsp1720I GCTNAGC 1 cut(s) 1188
BspACI CCGC 4 cut(s) 97, 108, 110, 380
BspANI GGCC 5 cut(s) 107, 215, 275, 379, 422
BspCNI CTCAG 6 cut(s) 214, 242, 403, 755, 1180, 1343
BspFNI CGCG 2 cut(s) 110, 1336
BspHI TCATGA 1 cut(s) 682
BspLI GGNNCC 3 cut(s) 447, 1544, 1545
BspMAI CTGCAG 1 cut(s) 390
BspOI GCTAGC 1 cut(s) 1488
BspPI GGATC 2 cut(s) 234, 452
BspQI GCTCTTC 1 cut(s) 539
BspT104I TTCGAA 5 cut(s) 371, 536, 542, 842, 1139
BspT107I GGYRCC 1 cut(s) 445
BspTNI GGTCTC 1 cut(s) 232
BsrDI GCAATG 1 cut(s) 1486
BsrFI RCCGGY 1 cut(s) 103
BsrI ACTGG 3 cut(s) 290, 1175, 1471
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 4 cut(s) 108, 403, 978, 1546
BssMI GATC 7 cut(s) 239, 294, 457, 938, 1222, 1396, 1456
BssT1I CCWWGG 1 cut(s) 403
Bst2UI CCWGG 1 cut(s) 1548
Bst4CI ACNGT 3 cut(s) 765, 1031, 1232
Bst6I CTCTTC 3 cut(s) 539, 639, 884
BstBI TTCGAA 5 cut(s) 371, 536, 542, 842, 1139
BstC8I GCNNGC 5 cut(s) 101, 105, 716, 1486, 1490
BstDEI CTNAG 8 cut(s) 222, 229, 411, 742, 999, 1167, 1188, 1330
BstDSI CCRYGG 2 cut(s) 108, 978
BstF5I GGATG 5 cut(s) 69, 511, 814, 913, 1252
BstFNI CGCG 2 cut(s) 110, 1336
BstHHI GCGC 1 cut(s) 1336
BstKTI GATC 7 cut(s) 242, 297, 460, 941, 1225, 1399, 1459
BstMAI GTCTC 3 cut(s) 212, 232, 905
BstMBI GATC 7 cut(s) 239, 294, 457, 938, 1222, 1396, 1456
BstMCI CGRYCG 2 cut(s) 108, 276
BstMWI GCNNNNNNNGC 5 cut(s) 127, 272, 319, 385, 1485
BstNI CCWGG 1 cut(s) 1548
BstPAI GACNNNNGTC 1 cut(s) 224
BstSCI CCNGG 1 cut(s) 1546
BstSFI CTRYAG 1 cut(s) 386
BstSLI GKGCMC 1 cut(s) 448
BstUI CGCG 2 cut(s) 110, 1336
BstV1I GCAGC 9 cut(s) 166, 278, 297, 298, 369, 372, 605, 1005, 1316
BstV2I GAAGAC 1 cut(s) 185
BstXI CCANNNNNNTGG 1 cut(s) 1415
BstZI CGGCCG 2 cut(s) 105, 273
BsuRI GGCC 5 cut(s) 107, 215, 275, 379, 422
BtgI CCRYGG 2 cut(s) 108, 978
BtgZI GCGATG 1 cut(s) 1351
BtsCI GGATG 5 cut(s) 69, 511, 814, 913, 1252
BtsI GCAGTG 1 cut(s) 1420
BtsIMutI CAGTG 4 cut(s) 297, 572, 1420, 1464
Cac8I GCNNGC 5 cut(s) 101, 105, 716, 1486, 1490
CciI TCATGA 1 cut(s) 682
CfoI GCGC 1 cut(s) 1336
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 3 cut(s) 989, 1270, 1543
Cfr42I CCGCGG 1 cut(s) 111
Csp6I GTAC 2 cut(s) 7, 361
CspCI CAANNNNNGTGG 2 cut(s) 960, 995
CviQI GTAC 2 cut(s) 7, 361
DdeI CTNAG 8 cut(s) 222, 229, 411, 742, 999, 1167, 1188, 1330
DpnI GATC 7 cut(s) 241, 296, 459, 940, 1224, 1398, 1458
DpnII GATC 7 cut(s) 239, 294, 457, 938, 1222, 1396, 1456
DraI TTTAAA 1 cut(s) 1501
EaeI YGGCCR 3 cut(s) 105, 213, 273
EagI CGGCCG 2 cut(s) 105, 273
Eam1104I CTCTTC 3 cut(s) 539, 639, 884
EarI CTCTTC 3 cut(s) 539, 639, 884
EclXI CGGCCG 2 cut(s) 105, 273
Eco130I CCWWGG 1 cut(s) 403
Eco31I GGTCTC 1 cut(s) 232
Eco47I GGWCC 3 cut(s) 989, 1270, 1543
Eco52I CGGCCG 2 cut(s) 105, 273
Eco57I CTGAAG 4 cut(s) 64, 116, 372, 596
Eco88I CYCGRG 1 cut(s) 1392
EcoO109I RGGNCCY 1 cut(s) 1543
EcoRI GAATTC 3 cut(s) 538, 1095, 1262
EcoRII CCWGG 1 cut(s) 1546
EcoT14I CCWWGG 1 cut(s) 403
ErhI CCWWGG 1 cut(s) 403
FaqI GGGAC 3 cut(s) 960, 1082, 1529
FbaI TGATCA 1 cut(s) 1456
FblI GTMKAC 1 cut(s) 1179
FokI GGATG 5 cut(s) 56, 518, 821, 920, 1259
FspBI CTAG 2 cut(s) 1308, 1485
GlaI GCGC 1 cut(s) 1335
GsaI CCCAGC 1 cut(s) 1496
HaeIII GGCC 5 cut(s) 107, 215, 275, 379, 422
HapII CCGG 2 cut(s) 104, 272
HhaI GCGC 1 cut(s) 1336
Hin6I GCGC 1 cut(s) 1334
HinP1I GCGC 1 cut(s) 1334
HindIII AAGCTT 5 cut(s) 128, 514, 962, 1001, 1169
HinfI GANTC 4 cut(s) 44, 161, 175, 1378
HpaII CCGG 2 cut(s) 104, 272
HphI GGTGA 6 cut(s) 43, 609, 995, 1244, 1264, 1364
Hpy166II GTNNAC 2 cut(s) 7, 1180
Hpy188I TCNGA 8 cut(s) 139, 352, 745, 783, 943, 1027, 1042, 1101
Hpy188III TCNNGA 6 cut(s) 88, 298, 683, 1009, 1092, 1352
Hpy8I GTNNAC 2 cut(s) 7, 1180
Hpy99I CGWCG 1 cut(s) 1361
HpyAV CCTTC 9 cut(s) 83, 88, 197, 496, 849, 980, 1047, 1075, 1546
HpyCH4III ACNGT 3 cut(s) 765, 1031, 1232
HpyF10VI GCNNNNNNNGC 5 cut(s) 127, 272, 319, 385, 1485
HpyF3I CTNAG 8 cut(s) 222, 229, 411, 742, 999, 1167, 1188, 1330
HspAI GCGC 1 cut(s) 1334
KflI GGGWCCC 1 cut(s) 1543
KroI GCCGGC 1 cut(s) 103
KroNI GCCGGC 1 cut(s) 105
Ksp22I TGATCA 1 cut(s) 1456
KspI CCGCGG 1 cut(s) 111
Kzo9I GATC 7 cut(s) 239, 294, 457, 938, 1222, 1396, 1456
LguI GCTCTTC 1 cut(s) 539
LmnI GCTCC 5 cut(s) 274, 407, 590, 651, 1084
Lsp1109I GCAGC 9 cut(s) 166, 278, 297, 298, 369, 372, 605, 1005, 1316
MaeI CTAG 2 cut(s) 1308, 1485
MaeIII GTNAC 3 cut(s) 49, 615, 1370
MalI GATC 7 cut(s) 241, 296, 459, 940, 1224, 1398, 1458
MboI GATC 7 cut(s) 239, 294, 457, 938, 1222, 1396, 1456
MboII GAAGA 9 cut(s) 185, 360, 365, 556, 646, 656, 892, 901, 1267
MhlI GDGCHC 1 cut(s) 448
MlyI GAGTC 2 cut(s) 53, 155
MroNI GCCGGC 1 cut(s) 103
MroXI GAANNNNTTC 3 cut(s) 1202, 1262, 1535
MseI TTAA 4 cut(s) 453, 585, 930, 1500
MslI CAYNNNNRTG 1 cut(s) 1413
MspA1I CMGCKG 2 cut(s) 110, 382
MspI CCGG 2 cut(s) 104, 272
MspR9I CCNGG 1 cut(s) 1548
Mva1269I GAATGC 2 cut(s) 475, 1306
MvaI CCWGG 1 cut(s) 1548
MvnI CGCG 2 cut(s) 110, 1336
MwoI GCNNNNNNNGC 5 cut(s) 127, 272, 319, 385, 1485
NaeI GCCGGC 1 cut(s) 105
NdeII GATC 7 cut(s) 239, 294, 457, 938, 1222, 1396, 1456
NgoMIV GCCGGC 1 cut(s) 103
NheI GCTAGC 1 cut(s) 1484
NlaIV GGNNCC 3 cut(s) 447, 1544, 1545
NmeAIII GCCGAG 2 cut(s) 241, 548
NmuCI GTSAC 3 cut(s) 49, 615, 1370
NspV TTCGAA 5 cut(s) 371, 536, 542, 842, 1139
PagI TCATGA 1 cut(s) 682
PciSI GCTCTTC 1 cut(s) 539
PcsI WCGNNNNNNNCGW 1 cut(s) 848
PctI GAATGC 2 cut(s) 475, 1306
PdiI GCCGGC 1 cut(s) 105
PdmI GAANNNNTTC 3 cut(s) 1202, 1262, 1535
PfeI GAWTC 2 cut(s) 175, 1378
PflMI CCANNNNNTGG 1 cut(s) 1215
PleI GAGTC 2 cut(s) 52, 155
PpsI GAGTC 2 cut(s) 52, 155
PpuMI RGGWCCY 1 cut(s) 1543
PshAI GACNNNNGTC 1 cut(s) 224
Psp5II RGGWCCY 1 cut(s) 1543
Psp6I CCWGG 1 cut(s) 1546
PspFI CCCAGC 1 cut(s) 1492
PspGI CCWGG 1 cut(s) 1546
PspN4I GGNNCC 3 cut(s) 447, 1544, 1545
PspPI GGNCC 3 cut(s) 989, 1270, 1543
PspPPI RGGWCCY 1 cut(s) 1543
PstI CTGCAG 1 cut(s) 390
RsaI GTAC 2 cut(s) 8, 362
RsaNI GTAC 2 cut(s) 7, 361
RseI CAYNNNNRTG 1 cut(s) 1413
SacII CCGCGG 1 cut(s) 111
SapI GCTCTTC 1 cut(s) 539
SaqAI TTAA 4 cut(s) 453, 585, 930, 1500
Sau3AI GATC 7 cut(s) 239, 294, 457, 938, 1222, 1396, 1456
Sau96I GGNCC 3 cut(s) 989, 1270, 1543
SchI GAGTC 2 cut(s) 53, 155
ScrFI CCNGG 1 cut(s) 1548
SduI GDGCHC 1 cut(s) 448
SfcI CTRYAG 1 cut(s) 386
Sfr303I CCGCGG 1 cut(s) 111
SfuI TTCGAA 5 cut(s) 371, 536, 542, 842, 1139
SgrBI CCGCGG 1 cut(s) 111
SinI GGWCC 3 cut(s) 989, 1270, 1543
SmiMI CAYNNNNRTG 1 cut(s) 1413
SmlI CTYRAG 2 cut(s) 298, 1294
SmoI CTYRAG 2 cut(s) 298, 1294
SsiI CCGC 4 cut(s) 97, 108, 110, 380
SspMI CTAG 2 cut(s) 1308, 1485
StyD4I CCNGG 1 cut(s) 1546
StyI CCWWGG 1 cut(s) 403
TaaI ACNGT 3 cut(s) 765, 1031, 1232
TaqII GACCGA 1 cut(s) 1118
TatI WGTACW 1 cut(s) 6
TauI GCSGC 3 cut(s) 99, 110, 382
TfiI GAWTC 2 cut(s) 175, 1378
Tru1I TTAA 4 cut(s) 453, 585, 930, 1500
Tru9I TTAA 4 cut(s) 453, 585, 930, 1500
TscAI CASTG 4 cut(s) 297, 579, 1420, 1471
TseFI GTSAC 3 cut(s) 49, 615, 1370
TseI GCWGC 9 cut(s) 154, 266, 286, 310, 382, 385, 593, 1018, 1304
Tsp45I GTSAC 3 cut(s) 49, 615, 1370
TspDTI ATGAA 8 cut(s) 536, 699, 944, 1039, 1340, 1377, 1392, 1449
TspGWI ACGGA 1 cut(s) 37
TspRI CASTG 4 cut(s) 297, 579, 1420, 1471
Van91I CCANNNNNTGG 1 cut(s) 1215
VpaK11BI GGWCC 3 cut(s) 989, 1270, 1543
XapI RAATTY 5 cut(s) 538, 772, 1095, 1133, 1262
XmiI GTMKAC 1 cut(s) 1179
XmnI GAANNNNTTC 3 cut(s) 1202, 1262, 1535
XspI CTAG 2 cut(s) 1308, 1485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.