MD15G1160100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0013577
Physical Location & Seq
Forward (+)
11986310 .. 11988657
2348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1160100.v1.1.491

Sequence Viewer

Length: 1611 bp
ATGGTGTATTCGTACGCGCCCACATACTACTCCACTCTTCACGACTCGATGACTTCCCTGTGCAAGTCCATTCTTCCCTTCGGCTTCAAGAAGCGGCGGTTGCCCGCGGCGGAGCAGAAGCTTTCGAAGCTGCAGTCGGATAACCTCAAGTGGCAGCAGGACTCTTTCCACCAGATGATGAACTTGATGGGTCTGCACAAAGAAGGAATCTTGGCTGAGACCGAGGTCTCCGCTTTCCGATTGCATTTGCTCGAAACCCTCCTCGCCTCTCCAGTGGCGGAGCACGAACAGCCGGTTATTCTGAGAGACAAGTTGCTATTCTTGCAGGAGCTCTTGTATGCGAAATGCATATCAGAAGACGAATACCATTCGTCGAAGAGGCCGTTGCTGCAGCGATTAGCAGCTCAGGGAGCTGAGGTCGAGGCTAAAGATGTAATCGCTTCAGGGTCGCTGCCAGCGAAGGACCTGAAAGAGAATGCAGATCAAGAGCAGTGGTCTGAAATTGACTTGAAGGATGAGAAATGCTTGCTAACGAAAGACAAAGGGGGTTCAAATTCGAAGAACAAATTGAAGCACAGCTCGGCAGTGAAGCAAATCAAAGGGGCAGCTTCGGTGTTGGGATTTGTATCACCTTACAAACAGGGGAAAAACAGGGGAGAAAAGAGCATATTTGATTTGCCCGACTCGTCGAAATTCTCTTCTGCCAGCTCTTCTAAGCATGAAGCAGGAAGTCAATCGTCGATACTCATGCCAGAAAGCTTGCCACAGGTTCCGATGAAAGAGAGTGGGAGCGCAGATCGAACGAGGAGGAAGCCGTTTAGGAATTTGTTTCAGAGAGAACAGAGAGAGGGACAGGGGGATCATGGCCATGAAAATGAACAAACACCTTCGAAATCTGCGAAAAAGCAATGGGGTTTTGATGGATTCAAGAAGTGGAAGAGGAGTGACTCAGAGGAGAACGAGACAACTCCGTTGCCACTAAACGAAAGATCGGATAGTGAGGTCCACCTGAAGCTTGTCCCAAGCCCCATGGGTGAAGGGCCAGACACCAAGCTCATAAAAAGGAAGCTGCTCTCGGATGGTTCTACTTCTGATTTCTTCATTGACAAGGTAGTGGGGGAGAAGATAAAGAAGGAGCTGTCAAGAATTCAGACAGAGCTTTGCGCTTCAAATCCAAATCTTCGATTCACGAATGAACAAATTGAAGCGATTTCAACAAGGCTTCCGGTGGACAAGGCTGACCTGAAGAAGTTCTTTCCTAAGTCGTGGTGTGATCGGTATGGAGACGTTGTTCTGGACGTGGTGAAGAAAGAATTCAAGGAGCATGTTGGGGAGATGGAGAGCTTGAGAAATGCAGCAAGAGAGAAGCACGGTATCCACTCAGCGCGATGGGTTACATTCCAAGATGACGAGGAGAATTGTCACCCGAATCTCTTTGCTCCGCCGTGTGACAACAACCACAATTACACCTCAAAGTTCCATGAAACCAATCCATTCTGTGATGATCACCTTCACACCACCCCTAGGAAGCTGAGATCGAAACCGGCAGCTTATACACAAGACCAGAATCAGAATCAGAATCCCTTTTGGATTCCGGGGCATGGCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

537

Amino Acids

60.88

Weight (kDa)

8.22

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013577)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01760 FvH4_1g01760 FvH4_1g01760
malus_domestica MD02G1013300.v1.1 MD15G1160100.v1.1
prunus_persica Prupe.7G255100_v2.0.a1
pyrus_communis pycom02g01240 pycom15g14400
rosa_chinensis RchiOBHm_Chr2g0086721
rosa_laevigata RLG00000015785
rosa_multiflora Rmu_sc0004626.1_g000002
rosa_roxburghii Rroxscaffold_2G00154250
rosa_rugosa Rorug01G0469200
rosa_samantha Rh2AG021200 Rh2BG021100 Rh2CG022000 Rh2DG021800
rosa_wichuraiana Rw2G001720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 17, 107, 1387
AciI CCGC 8 cut(s) 94, 97, 105, 107, 110, 231, 278, 1442
AclWI GGATC 1 cut(s) 867
AcoI YGGCCR 1 cut(s) 865
AcsI RAATTY 5 cut(s) 553, 692, 823, 1146, 1313
AcuI CTGAAG 3 cut(s) 426, 1031, 1265
AfaI GTAC 1 cut(s) 14
AfiI CCNNNNNNNGG 2 cut(s) 1524, 1601
AgsI TTSAA 9 cut(s) 88, 511, 552, 571, 928, 1170, 1205, 1215, 1318
AjiI CACGTC 1 cut(s) 1300
AloI GAACNNNNNNTCC 2 cut(s) 1275, 1307
Alw21I GWGCWC 2 cut(s) 285, 333
Alw26I GTCTC 5 cut(s) 212, 232, 300, 956, 1278
AlwI GGATC 1 cut(s) 867
AoxI GGCC 3 cut(s) 380, 865, 1040
ApoI RAATTY 5 cut(s) 553, 692, 823, 1146, 1313
ArsI GACNNNNNNTTYG 2 cut(s) 119, 151
Asp700I GAANNNNTTC 2 cut(s) 1250, 1313
AspA2I CCTAGG 1 cut(s) 1523
AspLEI GCGC 4 cut(s) 19, 794, 1166, 1387
AspS9I GGNCC 3 cut(s) 463, 1003, 1040
AsuC2I CCSGG 1 cut(s) 1596
AsuHPI GGTGA 5 cut(s) 621, 1046, 1315, 1415, 1499
AsuII TTCGAA 3 cut(s) 125, 557, 890
AvaII GGWCC 2 cut(s) 463, 1003
AvrII CCTAGG 1 cut(s) 1523
BalI TGGCCA 1 cut(s) 867
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 1 cut(s) 363
Bbv12I GWGCWC 2 cut(s) 285, 333
BbvCI CCTCAGC 1 cut(s) 414
BccI CCATC 5 cut(s) 181, 914, 1073, 1330, 1383
BceAI ACGGC 3 cut(s) 367, 799, 1429
BcgI CGANNNNNNTGC 2 cut(s) 730, 764
BciVI GTATCC 1 cut(s) 1385
BclI TGATCA 1 cut(s) 1504
BcnI CCSGG 1 cut(s) 1596
BcoDI GTCTC 5 cut(s) 212, 232, 300, 956, 1278
BfaI CTAG 1 cut(s) 1524
BfmI CTRYAG 2 cut(s) 131, 389
BfuI GTATCC 1 cut(s) 1385
BlnI CCTAGG 1 cut(s) 1523
Bme1390I CCNGG 1 cut(s) 1596
Bme18I GGWCC 2 cut(s) 463, 1003
BmgBI CACGTC 1 cut(s) 1300
BmgT120I GGNCC 3 cut(s) 463, 1003, 1040
BmiI GGNNCC 1 cut(s) 771
BmrFI CCNGG 1 cut(s) 1596
BoxI GACNNNNGTC 1 cut(s) 224
BpiI GAAGAC 1 cut(s) 363
BpmI CTGGAG 1 cut(s) 255
Bpu10I CCTNAGC 2 cut(s) 405, 414
Bpu14I TTCGAA 3 cut(s) 125, 557, 890
BpuEI CTTGAG 2 cut(s) 131, 1366
BpuMI CCSGG 1 cut(s) 1596
BsaBI GATNNNNATC 1 cut(s) 625
BsaI GGTCTC 2 cut(s) 212, 232
BsaJI CCNNGG 5 cut(s) 105, 222, 1029, 1523, 1595
BsaWI WCCGGW 1 cut(s) 1225
BsaXI ACNNNNNCTCC 6 cut(s) 320, 350, 402, 432, 1275, 1305
Bsc4I CCNNNNNNNGG 2 cut(s) 1524, 1601
Bse118I RCCGGY 2 cut(s) 292, 1543
Bse1I ACTGG 1 cut(s) 272
Bse3DI GCAATG 1 cut(s) 914
Bse8I GATNNNNATC 1 cut(s) 625
BseDI CCNNGG 5 cut(s) 105, 222, 1029, 1523, 1595
BseGI GGATG 2 cut(s) 520, 1084
BseJI GATNNNNATC 1 cut(s) 625
BseLI CCNNNNNNNGG 2 cut(s) 1524, 1601
BseMI GCAATG 1 cut(s) 914
BseMII CTCAG 7 cut(s) 207, 293, 405, 419, 963, 1395, 1523
BseNI ACTGG 1 cut(s) 272
BseRI GAGGAG 5 cut(s) 251, 820, 955, 968, 1427
BsgI GTGCAG 1 cut(s) 179
Bsh1236I CGCG 3 cut(s) 17, 107, 1387
BshFI GGCC 3 cut(s) 382, 867, 1042
BsiHKAI GWGCWC 2 cut(s) 285, 333
BsiSI CCGG 4 cut(s) 293, 1226, 1544, 1595
BsiWI CGTACG 1 cut(s) 12
BslFI GGGAC 2 cut(s) 864, 1004
BslI CCNNNNNNNGG 2 cut(s) 1524, 1601
BsmAI GTCTC 5 cut(s) 212, 232, 300, 956, 1278
BsmBI CGTCTC 1 cut(s) 1278
BsmFI GGGAC 2 cut(s) 864, 1004
BsmI GAATGC 1 cut(s) 481
BsnI GGCC 3 cut(s) 382, 867, 1042
Bso31I GGTCTC 2 cut(s) 212, 232
Bsp119I TTCGAA 3 cut(s) 125, 557, 890
Bsp1286I GDGCHC 2 cut(s) 285, 333
Bsp143I GATC 7 cut(s) 481, 796, 859, 989, 1273, 1504, 1535
Bsp19I CCATGG 1 cut(s) 1029
BspACI CCGC 8 cut(s) 94, 97, 105, 107, 110, 231, 278, 1442
BspANI GGCC 3 cut(s) 382, 867, 1042
BspCNI CTCAG 7 cut(s) 208, 294, 406, 418, 962, 1394, 1524
BspFNI CGCG 3 cut(s) 17, 107, 1387
BspLI GGNNCC 1 cut(s) 771
BspMAI CTGCAG 2 cut(s) 135, 393
BspPI GGATC 1 cut(s) 867
BspQI GCTCTTC 1 cut(s) 715
BspT104I TTCGAA 3 cut(s) 125, 557, 890
BspTNI GGTCTC 2 cut(s) 212, 232
BsrDI GCAATG 1 cut(s) 914
BsrFI RCCGGY 2 cut(s) 292, 1543
BsrI ACTGG 1 cut(s) 272
BssAI RCCGGY 2 cut(s) 292, 1543
BssECI CCNNGG 5 cut(s) 105, 222, 1029, 1523, 1595
BssMI GATC 7 cut(s) 481, 796, 859, 989, 1273, 1504, 1535
BssT1I CCWWGG 2 cut(s) 1029, 1523
Bst4CI ACNGT 1 cut(s) 1373
Bst6I CTCTTC 5 cut(s) 42, 371, 703, 715, 932
BstBI TTCGAA 3 cut(s) 125, 557, 890
BstC8I GCNNGC 5 cut(s) 105, 456, 527, 706, 761
BstDEI CTNAG 9 cut(s) 216, 302, 405, 414, 714, 949, 1260, 1381, 1532
BstDSI CCRYGG 2 cut(s) 105, 1029
BstF5I GGATG 2 cut(s) 520, 1084
BstFNI CGCG 3 cut(s) 17, 107, 1387
BstHHI GCGC 4 cut(s) 19, 794, 1166, 1387
BstKTI GATC 7 cut(s) 484, 799, 862, 992, 1276, 1507, 1538
BstMAI GTCTC 5 cut(s) 212, 232, 300, 956, 1278
BstMBI GATC 7 cut(s) 481, 796, 859, 989, 1273, 1504, 1535
BstMWI GCNNNNNNNGC 6 cut(s) 100, 127, 289, 322, 388, 410
BstNSI RCATGY 1 cut(s) 1328
BstPAI GACNNNNGTC 1 cut(s) 224
BstSCI CCNGG 1 cut(s) 1594
BstSFI CTRYAG 2 cut(s) 131, 389
BstUI CGCG 3 cut(s) 17, 107, 1387
BstV2I GAAGAC 1 cut(s) 363
BsuI GTATCC 1 cut(s) 1385
BsuRI GGCC 3 cut(s) 382, 867, 1042
BtgI CCRYGG 2 cut(s) 105, 1029
BtgZI GCGATG 1 cut(s) 1402
BtrI CACGTC 1 cut(s) 1300
BtsCI GGATG 2 cut(s) 520, 1084
BtsI GCAGTG 2 cut(s) 497, 591
BtsIMutI CAGTG 3 cut(s) 279, 497, 591
Cac8I GCNNGC 5 cut(s) 105, 456, 527, 706, 761
CfoI GCGC 4 cut(s) 19, 794, 1166, 1387
Cfr10I RCCGGY 2 cut(s) 292, 1543
Cfr13I GGNCC 3 cut(s) 463, 1003, 1040
Cfr42I CCGCGG 1 cut(s) 108
Csp6I GTAC 1 cut(s) 13
CspCI CAANNNNNGTGG 2 cut(s) 473, 508
CviAII CATG 8 cut(s) 719, 748, 863, 869, 1030, 1325, 1481, 1601
CviQI GTAC 1 cut(s) 13
DdeI CTNAG 9 cut(s) 216, 302, 405, 414, 714, 949, 1260, 1381, 1532
DpnI GATC 7 cut(s) 483, 798, 861, 991, 1275, 1506, 1537
DpnII GATC 7 cut(s) 481, 796, 859, 989, 1273, 1504, 1535
EaeI YGGCCR 1 cut(s) 865
Eam1104I CTCTTC 5 cut(s) 42, 371, 703, 715, 932
EarI CTCTTC 5 cut(s) 42, 371, 703, 715, 932
EciI GGCGGA 3 cut(s) 125, 293, 1431
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 2 cut(s) 1029, 1523
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 2 cut(s) 212, 232
Eco47I GGWCC 2 cut(s) 463, 1003
Eco53kI GAGCTC 1 cut(s) 331
Eco57I CTGAAG 3 cut(s) 426, 1031, 1265
EcoICRI GAGCTC 1 cut(s) 331
EcoO109I RGGNCCY 1 cut(s) 463
EcoRI GAATTC 2 cut(s) 1146, 1313
EcoT14I CCWWGG 2 cut(s) 1029, 1523
EcoT22I ATGCAT 1 cut(s) 350
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 2 cut(s) 1029, 1523
Esp3I CGTCTC 1 cut(s) 1278
FaeI CATG 8 cut(s) 722, 751, 866, 872, 1033, 1328, 1484, 1604
FalI AAGNNNNNCTT 2 cut(s) 1238, 1270
FaqI GGGAC 2 cut(s) 864, 1004
FatI CATG 8 cut(s) 718, 747, 862, 868, 1029, 1324, 1480, 1600
FauI CCCGC 1 cut(s) 112
FbaI TGATCA 1 cut(s) 1504
FokI GGATG 2 cut(s) 527, 1091
FriOI GRGCYC 1 cut(s) 333
FspBI CTAG 1 cut(s) 1524
GlaI GCGC 4 cut(s) 18, 793, 1165, 1386
GsuI CTGGAG 1 cut(s) 255
HaeIII GGCC 3 cut(s) 382, 867, 1042
HapII CCGG 4 cut(s) 293, 1226, 1544, 1595
HhaI GCGC 4 cut(s) 19, 794, 1166, 1387
Hin1II CATG 8 cut(s) 722, 751, 866, 872, 1033, 1328, 1484, 1604
Hin6I GCGC 4 cut(s) 17, 792, 1164, 1385
HinP1I GCGC 4 cut(s) 17, 792, 1164, 1385
HindIII AAGCTT 3 cut(s) 119, 757, 1013
HpaII CCGG 4 cut(s) 293, 1226, 1544, 1595
HphI GGTGA 5 cut(s) 621, 1046, 1315, 1415, 1499
Hpy166II GTNNAC 2 cut(s) 1006, 1231
Hpy188III TCNNGA 7 cut(s) 41, 88, 485, 928, 1143, 1189, 1295
Hpy8I GTNNAC 2 cut(s) 1006, 1231
Hpy99I CGWCG 3 cut(s) 376, 691, 742
HpyAV CCTTC 8 cut(s) 88, 197, 454, 505, 897, 1031, 1126, 1520
HpyCH4III ACNGT 1 cut(s) 1373
HpyCH4IV ACGT 2 cut(s) 1287, 1299
HpyCH4V TGCA 9 cut(s) 63, 133, 196, 244, 325, 348, 391, 479, 1355
HpyF10VI GCNNNNNNNGC 6 cut(s) 100, 127, 289, 322, 388, 410
HpyF3I CTNAG 9 cut(s) 216, 302, 405, 414, 714, 949, 1260, 1381, 1532
HpySE526I ACGT 2 cut(s) 1287, 1299
Hsp92II CATG 8 cut(s) 722, 751, 866, 872, 1033, 1328, 1484, 1604
HspAI GCGC 4 cut(s) 17, 792, 1164, 1385
Ksp22I TGATCA 1 cut(s) 1504
KspI CCGCGG 1 cut(s) 108
Kzo9I GATC 7 cut(s) 481, 796, 859, 989, 1273, 1504, 1535
LguI GCTCTTC 1 cut(s) 715
LmnI GCTCC 8 cut(s) 112, 280, 328, 410, 789, 1135, 1321, 1444
MaeI CTAG 1 cut(s) 1524
MaeII ACGT 2 cut(s) 1287, 1299
MaeIII GTNAC 4 cut(s) 944, 1393, 1421, 1448
MalI GATC 7 cut(s) 483, 798, 861, 991, 1275, 1506, 1537
MboI GATC 7 cut(s) 481, 796, 859, 989, 1273, 1504, 1535
MhlI GDGCHC 2 cut(s) 285, 333
MlsI TGGCCA 1 cut(s) 867
MluNI TGGCCA 1 cut(s) 867
MlyI GAGTC 4 cut(s) 38, 155, 677, 941
MmeI TCCRAC 1 cut(s) 117
Mox20I TGGCCA 1 cut(s) 867
Mph1103I ATGCAT 1 cut(s) 350
MroXI GAANNNNTTC 2 cut(s) 1250, 1313
MscI TGGCCA 1 cut(s) 867
MslI CAYNNNNRTG 2 cut(s) 867, 873
Msp20I TGGCCA 1 cut(s) 867
MspA1I CMGCKG 1 cut(s) 107
MspI CCGG 4 cut(s) 293, 1226, 1544, 1595
MspR9I CCNGG 1 cut(s) 1596
Mva1269I GAATGC 1 cut(s) 481
MvnI CGCG 3 cut(s) 17, 107, 1387
MwoI GCNNNNNNNGC 6 cut(s) 100, 127, 289, 322, 388, 410
NciI CCSGG 1 cut(s) 1596
NcoI CCATGG 1 cut(s) 1029
NdeII GATC 7 cut(s) 481, 796, 859, 989, 1273, 1504, 1535
NlaIII CATG 8 cut(s) 722, 751, 866, 872, 1033, 1328, 1484, 1604
NlaIV GGNNCC 1 cut(s) 771
NmeAIII GCCGAG 1 cut(s) 560
NmuCI GTSAC 3 cut(s) 944, 1421, 1448
NsiI ATGCAT 1 cut(s) 350
NspI RCATGY 1 cut(s) 1328
NspV TTCGAA 3 cut(s) 125, 557, 890
PciSI GCTCTTC 1 cut(s) 715
PcsI WCGNNNNNNNCGW 3 cut(s) 380, 455, 896
PctI GAATGC 1 cut(s) 481
PdmI GAANNNNTTC 2 cut(s) 1250, 1313
PfeI GAWTC 8 cut(s) 207, 924, 1185, 1429, 1567, 1573, 1579, 1591
Pfl23II CGTACG 1 cut(s) 12
PleI GAGTC 4 cut(s) 38, 155, 677, 941
PpsI GAGTC 4 cut(s) 38, 155, 677, 941
PpuMI RGGWCCY 1 cut(s) 463
PshAI GACNNNNGTC 1 cut(s) 224
Psp124BI GAGCTC 1 cut(s) 333
Psp5II RGGWCCY 1 cut(s) 463
PspLI CGTACG 1 cut(s) 12
PspN4I GGNNCC 1 cut(s) 771
PspPI GGNCC 3 cut(s) 463, 1003, 1040
PspPPI RGGWCCY 1 cut(s) 463
PstI CTGCAG 2 cut(s) 135, 393
RsaI GTAC 1 cut(s) 14
RsaNI GTAC 1 cut(s) 13
RseI CAYNNNNRTG 2 cut(s) 867, 873
SacI GAGCTC 1 cut(s) 333
SacII CCGCGG 1 cut(s) 108
SapI GCTCTTC 1 cut(s) 715
Sau3AI GATC 7 cut(s) 481, 796, 859, 989, 1273, 1504, 1535
Sau96I GGNCC 3 cut(s) 463, 1003, 1040
SchI GAGTC 4 cut(s) 38, 155, 677, 941
ScrFI CCNGG 1 cut(s) 1596
SduI GDGCHC 2 cut(s) 285, 333
SfcI CTRYAG 2 cut(s) 131, 389
Sfr303I CCGCGG 1 cut(s) 108
SfuI TTCGAA 3 cut(s) 125, 557, 890
SgrBI CCGCGG 1 cut(s) 108
SinI GGWCC 2 cut(s) 463, 1003
SmiMI CAYNNNNRTG 2 cut(s) 867, 873
SmlI CTYRAG 2 cut(s) 146, 1345
SmoI CTYRAG 2 cut(s) 146, 1345
SsiI CCGC 8 cut(s) 94, 97, 105, 107, 110, 231, 278, 1442
SspMI CTAG 1 cut(s) 1524
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 1 cut(s) 1594
StyI CCWWGG 2 cut(s) 1029, 1523
TaaI ACNGT 1 cut(s) 1373
TaiI ACGT 2 cut(s) 1290, 1302
TaqII GACCGA 1 cut(s) 236
TauI GCSGC 2 cut(s) 97, 110
TfiI GAWTC 8 cut(s) 207, 924, 1185, 1429, 1567, 1573, 1579, 1591
TscAI CASTG 3 cut(s) 279, 497, 591
TseFI GTSAC 3 cut(s) 944, 1421, 1448
Tsp45I GTSAC 3 cut(s) 944, 1421, 1448
TspDTI ATGAA 8 cut(s) 194, 735, 791, 885, 891, 1090, 1209, 1497
TspGWI ACGGA 1 cut(s) 960
TspRI CASTG 3 cut(s) 279, 497, 591
VpaK11BI GGWCC 2 cut(s) 463, 1003
XapI RAATTY 5 cut(s) 553, 692, 823, 1146, 1313
XceI RCATGY 1 cut(s) 1328
XmaJI CCTAGG 1 cut(s) 1523
XmnI GAANNNNTTC 2 cut(s) 1250, 1313
XspI CTAG 1 cut(s) 1524
Zsp2I ATGCAT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.