Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0004955

Physical Location & Seq

Genomic Coordinates Forward (+)

1486392 .. 1489889

Gene Structure 3498 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1020900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2343 bp

ATGCCTCTGGTTTCGCCTCGACCGTTGTCGGGCAGTGACAGTTTCTTTAGGGAGGAACCCTCCAATGAAGAGTTTGATGACCAAGGTGCTGCTCACTCGAACACTTTGGTAACACCACGCATCCGGCCTGCCCCATCAGCCTCATTTAATAGTGATCGGGAAAATCAGTCTGATCAGTCCCTGCAGCATGTTGTAGTTCAAAATTCTGATCATTCTCATAATCGCTCTGATAAGAAAAAGATGGATCCTTTGGTAAGAATTGATGATCTTCAAGTTAAGTTCTTGCGCCTCCTCCTCCGTCTTGGTCTGTCACAAAACAATGTTCTGGTTGCTAAGGTTCTTTATCGTATTCACCTGGCTACTTTGATACGAGCAGAAGAATCAGATTTGAAAAGAGTTAATCTGAGGAGTGATAGAGCTAGAGCCGTGGCAGCAGAACAGGAGGCATCAGGCCTGCCTGAAATGGATTTCTCTCTCAGAATCCTTGTCCTGGGGAAAACAGGAGTTGGCAAGAGTGCCACAATAAATTCTATATTTGATCAAACAAAGACTGAAACCAATCCATTTCGACCTGGCACAGATCGGATCCGAGAGGTTGTGGGAACTGTAAATGGGATTAGAGTAACTATCATCGATACCCCTGGTTTTTTGCCATCATGTACTGGTAATTTCCGCAGAAATAAGAAGATTATGCTCTCTGTGAAGAAATTTATTAGAAAAAGCCCACCTGACATTGTTTTGTTCTTCGAACGCCTCGACCTCATGAGTACTAATTACAATGACTTCTCCCTTTTGAAGCAAATAACCGAGGTATTTGGGCCTGCAGTTTGGTTTAACACCGTTCTTGTCATGACACATTCTTCCCTAGTCCTTCCGGAAGGACCTGATGGGTATCCTGTCAATTATGAATCATATGTGAGGCAAAGCACAGATATGGTGCAGCACTATATACACCAGGCAGTGTCTGACTCCAGACTTGAAAACCCAGTGTTGTTAGTTGAGAATCATCCTCAGTGTAGGAAAAATATCGCCGGTGAAAAGATACTTCCAAACGGACAGGTTTGGAAATCTCAGTTTTTGTTATTATGCATTTGTACTAAAGTTCTGAGTGATGTCAATATCCTCATGAAATTTCAAGACAGCATTCATCTGGGGCCCTCAAATGCCACTCACATGCCTTCTCTGCCGCATCTCCTATCGTCTCTTCTACGTCATCGCACTGCTATTGGTCCAAGTGGAGGGGATGTTGGAGTTGATGAGAGCTTGGTTTCAGATGCGGAGGACGAAGATGAGTATGATCAATTACCTCCAATCCGAATTCTGACAAAATCTCAGTTTGAGAGATTAACAAAATCACAGAAAAAAGACTATCTTGATGAATTGGATTATCGAGAGACCCTTTATCTGAAGAAACAGTTGAAAGAAGAGTACCGTAGGCAGATGGAGATTAAGCTTTCCAAAGAGAAAAATACGGCGAGTGATGATAATTCGAATAGTCAGCAGGCTTCCCAAGAAGAGGCTGTTTTATTACCAGATATGGAGGTCCCTCCAAGTTTTGGCTCTGATTGGCCTGCACACAGGTATCGTTGTCTTCTTACAAGTGATCAGTGGATTATGAGACCTGTTCTTGATTCCCATGGATGGGATAATGATGTGGGCTTGGATGGGGTAAGCTTGGAAACTGCTATGCAGGTAAATAGGAACGTGTTTACCTCTGTTGCAGGGCAGATGAGCAAGGACAAGCAGGATTTTAGCATTCAATCCGAGTGTGCTGCAGCTTACCTAGACCCCAATGGGACCGCATATTCTGTAGGTCTTGATGTTCAGTCTGCTGGTAGAGATACCATCTATACGCTTCACGGCAACACGAAGCTGAGGAAGTTGTGGCACAATGTTGCTGAAAGTGGATTCTCTTTGACGTCTTTTGGGAACAAGTATTACATTGGTGGCAAGCTTGAAGACACCATATCAGTTGGGAAGAGATTGAAATTTGTGATGAATGTTGGCCAAATGATGGGTCCTGAGCAAGTGGCATATGGTGGCGGTGTTGAAGCTACGTTGAGGGGTAGAGACTACCCTGTGAGAAATGATAATGTGAGCCTGATGATGACTATACTGTCTTTCAATAAAGAGATGGTGTTGGGTGGAAACTTACAGTCTGAGTCCCGGCTTGGGCGAAATATGAGTGTGTCGGTTAATGCTAATCTGAACAGCCGCAGGATGGGTAAGATAAGCATCAAAGCAAGTAGCACTGATCATTTGCAGTTCAGTATGGTTGCGGCTTTCACAATTTTCTGGGCCCTTTTACGGAAGAAGGATGTTAAAAGTACAAGTGATGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006605 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0015031 GO:0015833 GO:0033036 GO:0033365 GO:0034613 GO:0042886 GO:0045036 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072596 GO:0072598

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AIG1 TOC159_MAD

Protein Analysis

781

Amino Acids

87.54

Weight (kDa)

6.83

Isoelectric Point (pI)

45.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AIG1	PF04548	159 - 294	7.3e-19	AIG1 family
MMR_HSR1	PF01926	160 - 250	1.2e-08	50S ribosome-binding GTPase
TOC159_MAD	PF11886	506 - 770	3.7e-112	Translocase of chloroplast 159/132, membrane anchor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004955)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20300 AT5G20300 AT5G20300 AT5G20300 AT5G20300
fragaria_vesca	FvH4_1g02420 FvH4_1g02420 FvH4_1g02420 FvH4_1g02420 FvH4_1g02420 FvH4_1g02420 FvH4_1g02420 FvH4_1g02420
malus_domestica	MD02G1020900.v1.1 MD15G1164100.v1.1
prunus_persica	Prupe.7G250500_v2.0.a1 Prupe.7G250500_v2.0.a1
pyrus_communis	pycom02g01840 pycom15g14720
rosa_chinensis	RchiOBHm_Chr2g0087381
rosa_laevigata	RLG00000015861
rosa_multiflora	Rmu_sc0012811.1_g000001
rosa_roxburghii	Rroxscaffold_2G00153590
rosa_rugosa	Rorug01G0474600 Rorug01G0474700
rosa_samantha	Rh2AG028200 Rh2BG028100 Rh2CG028400 Rh2DG028200
rosa_wichuraiana	Rw2G002240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2116
AatII	GACGTC	1 cut(s)	1922
Acc36I	ACCTGC	1 cut(s)	1681
AccB7I	CCANNNNNTGG	2 cut(s)	1556, 2014
AccIII	TCCGGA	1 cut(s)	874
AciI	CCGC	7 cut(s)	673, 1187, 1277, 1800, 2043, 2215, 2279
AclWI	GGATC	4 cut(s)	239, 252, 580, 593
AcoI	YGGCCR	1 cut(s)	2005
AcsI	RAATTY	6 cut(s)	202, 526, 707, 1130, 1317, 1988
AcuI	CTGAAG	1 cut(s)	1427
AcyI	GRCGYC	1 cut(s)	1919
AfaI	GTAC	5 cut(s)	661, 769, 1096, 1430, 2329
AflIII	ACRYGT	1 cut(s)	1704
AjnI	CCWGG	5 cut(s)	354, 489, 571, 640, 954
AluBI	AGCT	8 cut(s)	419, 1263, 1453, 1674, 1778, 1873, 1954, 2054
AluI	AGCT	8 cut(s)	419, 1263, 1453, 1674, 1778, 1873, 1954, 2054
Alw26I	GTCTC	4 cut(s)	1206, 1388, 1612, 2064
AlwI	GGATC	4 cut(s)	239, 252, 580, 593
AlwNI	CAGNNNCTG	2 cut(s)	181, 965
Aor13HI	TCCGGA	1 cut(s)	874
AoxI	GGCC	7 cut(s)	125, 451, 818, 1154, 1568, 2005, 2298
ApaI	GGGCCC	2 cut(s)	1158, 2302
ApeKI	GCWGC	6 cut(s)	89, 184, 431, 940, 1772, 1775
ApoI	RAATTY	6 cut(s)	202, 526, 707, 1130, 1317, 1988
ArsI	GACNNNNNNTTYG	2 cut(s)	2002, 2034
AspLEI	GCGC	1 cut(s)	288
AsuC2I	CCSGG	1 cut(s)	2167
AsuHPI	GGTGA	2 cut(s)	344, 1046
AsuII	TTCGAA	2 cut(s)	747, 1490
AvaII	GGWCC	5 cut(s)	881, 1229, 1543, 1797, 2018
BaeGI	GKGCMC	2 cut(s)	1158, 2302
BaeI	ACNNNNGTAYC	2 cut(s)	1565, 1598
BalI	TGGCCA	1 cut(s)	2007
BamHI	GGATCC	2 cut(s)	244, 585
BanII	GRGCYC	2 cut(s)	1158, 2302
BbsI	GAAGAC	2 cut(s)	1583, 1965
BbvCI	CCTCAGC	1 cut(s)	1874
BbvI	GCAGC	6 cut(s)	76, 196, 443, 952, 1759, 1787
BceAI	ACGGC	3 cut(s)	410, 1488, 1876
BcgI	CGANNNNNNTGC	2 cut(s)	1754, 1788
BciT130I	CCWGG	5 cut(s)	356, 491, 573, 642, 956
BciVI	GTATCC	1 cut(s)	903
BclI	TGATCA	6 cut(s)	172, 208, 538, 1297, 1603, 2254
BcnI	CCSGG	1 cut(s)	2167
BcoDI	GTCTC	4 cut(s)	1206, 1388, 1612, 2064
BfaI	CTAG	3 cut(s)	420, 866, 1784
BfmI	CTRYAG	4 cut(s)	182, 822, 1773, 1809
BfuAI	ACCTGC	1 cut(s)	1681
BfuI	GTATCC	1 cut(s)	903
BisI	GCNGC	9 cut(s)	90, 185, 432, 941, 1187, 1773, 1776, 2215, 2280
BlsI	GCNGC	9 cut(s)	91, 186, 433, 942, 1188, 1774, 1777, 2216, 2281
BmcAI	AGTACT	1 cut(s)	769
Bme1390I	CCNGG	6 cut(s)	356, 491, 573, 642, 956, 2167
Bme18I	GGWCC	5 cut(s)	881, 1229, 1543, 1797, 2018
BmiI	GGNNCC	9 cut(s)	57, 246, 587, 1155, 1156, 1545, 1798, 2019, 2300
BmrFI	CCNGG	6 cut(s)	356, 491, 573, 642, 956, 2167
BmrI	ACTGGG	1 cut(s)	980
BmsI	GCATC	5 cut(s)	129, 455, 1198, 1264, 2244
BmuI	ACTGGG	1 cut(s)	980
BoxI	GACNNNNGTC	1 cut(s)	25
BpiI	GAAGAC	2 cut(s)	1583, 1965
BplI	GAGNNNNNCTC	2 cut(s)	44, 76
BpmI	CTGGAG	1 cut(s)	955
Bpu10I	CCTNAGC	3 cut(s)	333, 1874, 2022
Bpu14I	TTCGAA	2 cut(s)	747, 1490
BpuMI	CCSGG	1 cut(s)	2167
Bsa29I	ATCGAT	1 cut(s)	633
BsaBI	GATNNNNATC	2 cut(s)	891, 2234
BsaHI	GRCGYC	1 cut(s)	1919
BsaI	GGTCTC	2 cut(s)	1388, 1612
BsaJI	CCNNGG	6 cut(s)	82, 426, 490, 640, 807, 1636
BsaWI	WCCGGW	1 cut(s)	874
BsaXI	ACNNNNNCTCC	2 cut(s)	1230, 1260
Bse118I	RCCGGY	1 cut(s)	1031
Bse1I	ACTGG	2 cut(s)	667, 986
Bse8I	GATNNNNATC	2 cut(s)	891, 2234
BseAI	TCCGGA	1 cut(s)	874
BseBI	CCWGG	5 cut(s)	356, 491, 573, 642, 956
BseCI	ATCGAT	1 cut(s)	633
BseDI	CCNNGG	6 cut(s)	82, 426, 490, 640, 807, 1636
BseGI	GGATG	7 cut(s)	120, 1006, 1249, 1646, 1669, 2226, 2323
BseJI	GATNNNNATC	2 cut(s)	891, 2234
BseMII	CTCAG	9 cut(s)	395, 490, 1025, 1085, 1097, 1346, 1865, 2013, 2151
BseNI	ACTGG	2 cut(s)	667, 986
BseRI	GAGGAG	3 cut(s)	281, 284, 421
BseSI	GKGCMC	2 cut(s)	1158, 2302
BseXI	GCAGC	6 cut(s)	76, 196, 443, 952, 1759, 1787
BsgI	GTGCAG	2 cut(s)	959, 1557
Bsh1285I	CGRYCG	1 cut(s)	23
BshFI	GGCC	7 cut(s)	127, 453, 820, 1156, 1570, 2007, 2300
BshVI	ATCGAT	1 cut(s)	633
BsiEI	CGRYCG	1 cut(s)	23
BsiSI	CCGG	4 cut(s)	124, 875, 1032, 2167
BslFI	GGGAC	4 cut(s)	163, 1529, 1810, 2149
BsmAI	GTCTC	4 cut(s)	1206, 1388, 1612, 2064
BsmBI	CGTCTC	1 cut(s)	1206
BsmFI	GGGAC	4 cut(s)	163, 1529, 1810, 2149
BsmI	GAATGC	2 cut(s)	1143, 1755
BsnI	GGCC	7 cut(s)	127, 453, 820, 1156, 1570, 2007, 2300
Bso31I	GGTCTC	2 cut(s)	1388, 1612
Bsp119I	TTCGAA	2 cut(s)	747, 1490
Bsp120I	GGGCCC	2 cut(s)	1154, 2298
Bsp1286I	GDGCHC	2 cut(s)	1158, 2302
Bsp13I	TCCGGA	1 cut(s)	874
Bsp19I	CCATGG	1 cut(s)	1636
BspACI	CCGC	7 cut(s)	673, 1187, 1277, 1800, 2043, 2215, 2279
BspANI	GGCC	7 cut(s)	127, 453, 820, 1156, 1570, 2007, 2300
BspCNI	CTCAG	9 cut(s)	396, 489, 1024, 1084, 1098, 1345, 1866, 2014, 2152
BspDI	ATCGAT	1 cut(s)	633
BspEI	TCCGGA	1 cut(s)	874
BspHI	TCATGA	3 cut(s)	762, 849, 1125
BspLI	GGNNCC	9 cut(s)	57, 246, 587, 1155, 1156, 1545, 1798, 2019, 2300
BspMAI	CTGCAG	3 cut(s)	186, 826, 1777
BspMI	ACCTGC	1 cut(s)	1681
BspPI	GGATC	4 cut(s)	239, 252, 580, 593
BspT104I	TTCGAA	2 cut(s)	747, 1490
BspTNI	GGTCTC	2 cut(s)	1388, 1612
BsrFI	RCCGGY	1 cut(s)	1031
BsrI	ACTGG	2 cut(s)	667, 986
BssAI	RCCGGY	1 cut(s)	1031
BssECI	CCNNGG	6 cut(s)	82, 426, 490, 640, 807, 1636
BssNI	GRCGYC	1 cut(s)	1919
BssT1I	CCWWGG	2 cut(s)	82, 1636
Bst2UI	CCWGG	5 cut(s)	356, 491, 573, 642, 956
Bst4CI	ACNGT	8 cut(s)	24, 41, 607, 841, 1416, 1433, 2118, 2157
Bst6I	CTCTTC	5 cut(s)	63, 1209, 1419, 1509, 1973
BstACI	GRCGYC	1 cut(s)	1919
BstBI	TTCGAA	2 cut(s)	747, 1490
BstC8I	GCNNGC	6 cut(s)	129, 455, 822, 1503, 1572, 1952
BstDSI	CCRYGG	2 cut(s)	426, 1636
BstF5I	GGATG	7 cut(s)	120, 1006, 1249, 1646, 1669, 2226, 2323
BstHHI	GCGC	1 cut(s)	288
BstMAI	GTCTC	4 cut(s)	1206, 1388, 1612, 2064
BstMCI	CGRYCG	1 cut(s)	23
BstMWI	GCNNNNNNNGC	3 cut(s)	137, 431, 1183
BstNI	CCWGG	5 cut(s)	356, 491, 573, 642, 956
BstNSI	RCATGY	2 cut(s)	191, 1177
BstPAI	GACNNNNGTC	1 cut(s)	25
BstSCI	CCNGG	6 cut(s)	354, 489, 571, 640, 954, 2165
BstSFI	CTRYAG	4 cut(s)	182, 822, 1773, 1809
BstSLI	GKGCMC	2 cut(s)	1158, 2302
BstV1I	GCAGC	6 cut(s)	76, 196, 443, 952, 1759, 1787
BstV2I	GAAGAC	2 cut(s)	1583, 1965
BstX2I	RGATCY	2 cut(s)	244, 585
BstYI	RGATCY	2 cut(s)	244, 585
Bsu15I	ATCGAT	1 cut(s)	633
BsuI	GTATCC	1 cut(s)	903
BsuRI	GGCC	7 cut(s)	127, 453, 820, 1156, 1570, 2007, 2300
BsuTUI	ATCGAT	1 cut(s)	633
BtgI	CCRYGG	2 cut(s)	426, 1636
BtgZI	GCGATG	1 cut(s)	1199
BtsCI	GGATG	7 cut(s)	120, 1006, 1249, 1646, 1669, 2226, 2323
BtsI	GCAGTG	3 cut(s)	40, 966, 1218
BtsIMutI	CAGTG	7 cut(s)	40, 966, 993, 1019, 1218, 1613, 2250
BveI	ACCTGC	1 cut(s)	1681
Cac8I	GCNNGC	6 cut(s)	129, 455, 822, 1503, 1572, 1952
CaiI	CAGNNNCTG	2 cut(s)	181, 965
CciI	TCATGA	3 cut(s)	762, 849, 1125
CfoI	GCGC	1 cut(s)	288
Cfr10I	RCCGGY	1 cut(s)	1031
ClaI	ATCGAT	1 cut(s)	633
Csp6I	GTAC	5 cut(s)	660, 768, 1095, 1429, 2328
CviAII	CATG	7 cut(s)	188, 657, 763, 850, 1126, 1174, 1637
CviQI	GTAC	5 cut(s)	660, 768, 1095, 1429, 2328
DrdI	GACNNNNNNGTC	1 cut(s)	2116
DseDI	GACNNNNNNGTC	1 cut(s)	2116
EaeI	YGGCCR	1 cut(s)	2005
Eam1104I	CTCTTC	5 cut(s)	63, 1209, 1419, 1509, 1973
EarI	CTCTTC	5 cut(s)	63, 1209, 1419, 1509, 1973
Eco130I	CCWWGG	2 cut(s)	82, 1636
Eco147I	AGGCCT	1 cut(s)	453
Eco24I	GRGCYC	2 cut(s)	1158, 2302
Eco31I	GGTCTC	2 cut(s)	1388, 1612
Eco47I	GGWCC	5 cut(s)	881, 1229, 1543, 1797, 2018
Eco57I	CTGAAG	1 cut(s)	1427
EcoO109I	RGGNCCY	6 cut(s)	881, 1154, 1155, 1543, 2018, 2299
EcoRI	GAATTC	1 cut(s)	1317
EcoRII	CCWGG	5 cut(s)	354, 489, 571, 640, 954
EcoT14I	CCWWGG	2 cut(s)	82, 1636
EcoT22I	ATGCAT	1 cut(s)	1091
EcoT38I	GRGCYC	2 cut(s)	1158, 2302
ErhI	CCWWGG	2 cut(s)	82, 1636
Esp3I	CGTCTC	1 cut(s)	1206
FaeI	CATG	7 cut(s)	191, 660, 766, 853, 1129, 1177, 1640
FalI	AAGNNNNNCTT	2 cut(s)	1356, 1388
FaqI	GGGAC	4 cut(s)	163, 1529, 1810, 2149
FatI	CATG	7 cut(s)	187, 656, 762, 849, 1125, 1173, 1636
FauNDI	CATATG	2 cut(s)	913, 2035
FbaI	TGATCA	6 cut(s)	172, 208, 538, 1297, 1603, 2254
Fnu4HI	GCNGC	9 cut(s)	90, 185, 432, 941, 1187, 1773, 1776, 2215, 2280
FokI	GGATG	7 cut(s)	107, 993, 1256, 1653, 1676, 2233, 2330
FriOI	GRGCYC	2 cut(s)	1158, 2302
Fsp4HI	GCNGC	9 cut(s)	90, 185, 432, 941, 1187, 1773, 1776, 2215, 2280
FspBI	CTAG	3 cut(s)	420, 866, 1784
GlaI	GCGC	1 cut(s)	287
GluI	GCNGC	9 cut(s)	90, 185, 432, 941, 1187, 1773, 1776, 2215, 2280
GsuI	CTGGAG	1 cut(s)	955
HaeIII	GGCC	7 cut(s)	127, 453, 820, 1156, 1570, 2007, 2300
HapII	CCGG	4 cut(s)	124, 875, 1032, 2167
HhaI	GCGC	1 cut(s)	288
Hin1I	GRCGYC	1 cut(s)	1919
Hin1II	CATG	7 cut(s)	191, 660, 766, 853, 1129, 1177, 1640
Hin6I	GCGC	1 cut(s)	286
HinP1I	GCGC	1 cut(s)	286
HindIII	AAGCTT	3 cut(s)	1451, 1672, 1952
HinfI	GANTC	8 cut(s)	380, 480, 908, 968, 1003, 1631, 1908, 2162
HpaII	CCGG	4 cut(s)	124, 875, 1032, 2167
HphI	GGTGA	2 cut(s)	344, 1046
Hpy166II	GTNNAC	1 cut(s)	1710
Hpy8I	GTNNAC	1 cut(s)	1710
HpyAV	CCTTC	4 cut(s)	872, 881, 1188, 2308
HpyCH4III	ACNGT	8 cut(s)	24, 41, 607, 841, 1416, 1433, 2118, 2157
HpyCH4IV	ACGT	4 cut(s)	1210, 1704, 1919, 2057
HpyCH4V	TGCA	9 cut(s)	184, 824, 940, 1089, 1574, 1690, 1721, 1775, 2263
HpyF10VI	GCNNNNNNNGC	3 cut(s)	137, 431, 1183
HpySE526I	ACGT	4 cut(s)	1210, 1704, 1919, 2057
Hsp92I	GRCGYC	1 cut(s)	1919
Hsp92II	CATG	7 cut(s)	191, 660, 766, 853, 1129, 1177, 1640
HspAI	GCGC	1 cut(s)	286
Kpn2I	TCCGGA	1 cut(s)	874
Ksp22I	TGATCA	6 cut(s)	172, 208, 538, 1297, 1603, 2254
Lsp1109I	GCAGC	6 cut(s)	76, 196, 443, 952, 1759, 1787
LweI	GCATC	5 cut(s)	129, 455, 1198, 1264, 2244
MaeI	CTAG	3 cut(s)	420, 866, 1784
MaeII	ACGT	4 cut(s)	1210, 1704, 1919, 2057
MaeIII	GTNAC	4 cut(s)	35, 109, 309, 622
MflI	RGATCY	2 cut(s)	244, 585
MhlI	GDGCHC	2 cut(s)	1158, 2302
MlsI	TGGCCA	1 cut(s)	2007
MluNI	TGGCCA	1 cut(s)	2007
MlyI	GAGTC	2 cut(s)	962, 2171
MmeI	TCCRAC	1 cut(s)	1228
Mox20I	TGGCCA	1 cut(s)	2007
Mph1103I	ATGCAT	1 cut(s)	1091
MroI	TCCGGA	1 cut(s)	874
MscI	TGGCCA	1 cut(s)	2007
MseI	TTAA	8 cut(s)	147, 276, 399, 834, 1346, 1449, 2196, 2322
MslI	CAYNNNNRTG	2 cut(s)	932, 1172
Msp20I	TGGCCA	1 cut(s)	2007
MspI	CCGG	4 cut(s)	124, 875, 1032, 2167
MspR9I	CCNGG	6 cut(s)	356, 491, 573, 642, 956, 2167
Mva1269I	GAATGC	2 cut(s)	1143, 1755
MvaI	CCWGG	5 cut(s)	356, 491, 573, 642, 956
MwoI	GCNNNNNNNGC	3 cut(s)	137, 431, 1183
NciI	CCSGG	1 cut(s)	2167
NcoI	CCATGG	1 cut(s)	1636
NdeI	CATATG	2 cut(s)	913, 2035
NlaIII	CATG	7 cut(s)	191, 660, 766, 853, 1129, 1177, 1640
NlaIV	GGNNCC	9 cut(s)	57, 246, 587, 1155, 1156, 1545, 1798, 2019, 2300
NmuCI	GTSAC	2 cut(s)	35, 309
NsiI	ATGCAT	1 cut(s)	1091
NspI	RCATGY	2 cut(s)	191, 1177
NspV	TTCGAA	2 cut(s)	747, 1490
PagI	TCATGA	3 cut(s)	762, 849, 1125
PceI	AGGCCT	1 cut(s)	453
PcsI	WCGNNNNNNNCGW	2 cut(s)	20, 753
PctI	GAATGC	2 cut(s)	1143, 1755
PfeI	GAWTC	6 cut(s)	380, 480, 908, 1003, 1631, 1908
PflMI	CCANNNNNTGG	2 cut(s)	1556, 2014
PkrI	GCNGC	9 cut(s)	91, 186, 433, 942, 1188, 1774, 1777, 2216, 2281
PleI	GAGTC	2 cut(s)	962, 2170
PpsI	GAGTC	2 cut(s)	962, 2170
PpuMI	RGGWCCY	3 cut(s)	881, 1543, 2018
PshAI	GACNNNNGTC	1 cut(s)	25
Psp5II	RGGWCCY	3 cut(s)	881, 1543, 2018
Psp6I	CCWGG	5 cut(s)	354, 489, 571, 640, 954
PspGI	CCWGG	5 cut(s)	354, 489, 571, 640, 954
PspN4I	GGNNCC	9 cut(s)	57, 246, 587, 1155, 1156, 1545, 1798, 2019, 2300
PspOMI	GGGCCC	2 cut(s)	1154, 2298
PspPPI	RGGWCCY	3 cut(s)	881, 1543, 2018
PsrI	GAACNNNNNNTAC	2 cut(s)	1922, 1954
PstI	CTGCAG	3 cut(s)	186, 826, 1777
PstNI	CAGNNNCTG	2 cut(s)	181, 965
PsuI	RGATCY	2 cut(s)	244, 585
RsaI	GTAC	5 cut(s)	661, 769, 1096, 1430, 2329
RsaNI	GTAC	5 cut(s)	660, 768, 1095, 1429, 2328
RseI	CAYNNNNRTG	2 cut(s)	932, 1172
SaqAI	TTAA	8 cut(s)	147, 276, 399, 834, 1346, 1449, 2196, 2322
SatI	GCNGC	9 cut(s)	90, 185, 432, 941, 1187, 1773, 1776, 2215, 2280
ScaI	AGTACT	1 cut(s)	769
SchI	GAGTC	2 cut(s)	962, 2171
ScrFI	CCNGG	6 cut(s)	356, 491, 573, 642, 956, 2167
SduI	GDGCHC	2 cut(s)	1158, 2302
SfaNI	GCATC	5 cut(s)	129, 455, 1198, 1264, 2244
SfcI	CTRYAG	4 cut(s)	182, 822, 1773, 1809
SfuI	TTCGAA	2 cut(s)	747, 1490
SgrAI	CRCCGGYG	1 cut(s)	1031
SinI	GGWCC	5 cut(s)	881, 1229, 1543, 1797, 2018
SmiMI	CAYNNNNRTG	2 cut(s)	932, 1172
SseBI	AGGCCT	1 cut(s)	453
SsiI	CCGC	7 cut(s)	673, 1187, 1277, 1800, 2043, 2215, 2279
SspMI	CTAG	3 cut(s)	420, 866, 1784
StuI	AGGCCT	1 cut(s)	453
StyD4I	CCNGG	6 cut(s)	354, 489, 571, 640, 954, 2165
StyI	CCWWGG	2 cut(s)	82, 1636
TaaI	ACNGT	8 cut(s)	24, 41, 607, 841, 1416, 1433, 2118, 2157
TaiI	ACGT	4 cut(s)	1213, 1707, 1922, 2060
TaqI	TCGA	8 cut(s)	19, 98, 568, 633, 747, 756, 1390, 1490
TatI	WGTACW	4 cut(s)	659, 767, 1094, 2327
TauI	GCSGC	3 cut(s)	1189, 2217, 2282
TfiI	GAWTC	6 cut(s)	380, 480, 908, 1003, 1631, 1908
Tru1I	TTAA	8 cut(s)	147, 276, 399, 834, 1346, 1449, 2196, 2322
Tru9I	TTAA	8 cut(s)	147, 276, 399, 834, 1346, 1449, 2196, 2322
TscAI	CASTG	7 cut(s)	40, 966, 993, 1019, 1225, 1613, 2257
TseFI	GTSAC	2 cut(s)	35, 309
TseI	GCWGC	6 cut(s)	89, 184, 431, 940, 1772, 1775
Tsp45I	GTSAC	2 cut(s)	35, 309
TspDTI	ATGAA	6 cut(s)	81, 921, 1136, 1142, 1392, 2012
TspGWI	ACGGA	3 cut(s)	287, 1068, 2323
TspRI	CASTG	7 cut(s)	40, 966, 993, 1019, 1225, 1613, 2257
Van91I	CCANNNNNTGG	2 cut(s)	1556, 2014
VpaK11BI	GGWCC	5 cut(s)	881, 1229, 1543, 1797, 2018
XapI	RAATTY	6 cut(s)	202, 526, 707, 1130, 1317, 1988
XceI	RCATGY	2 cut(s)	191, 1177
XspI	CTAG	3 cut(s)	420, 866, 1784
ZraI	GACGTC	1 cut(s)	1920
ZrmI	AGTACT	1 cut(s)	769
Zsp2I	ATGCAT	1 cut(s)	1091

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.