Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0004955

Physical Location & Seq

Genomic Coordinates Forward (+)

12355237 .. 12358766

Gene Structure 3530 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1164100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2343 bp

ATGCCTTTGGTTTTGCCTCGGCCATTGTCGGGCAGTGACAATTTCTTTAGAGAGGAATCCTCCAATGAAGAGTTTGATGACCAAGGTGCTGCTCACTCGAACACTTTGGTAACACCACGCATCCATCCTGCCGCATCAACCTCATTTAATAGTGATCAGGAAAATCAGTCTGGTCCTTCCCTGCAGCATGCTGTAGTTGAAAATTTGGATCACTCTCATAATCGCTCTGGTAAGAAAAAGATGGATCCTTTGGTAAGAATTGACGATCTTCAAGTTAAGTTTTTGCGCCTCCTCTTACGGCTTGGTCTGTCGCAGAACAATGTTCTGGTGGCTAAGGTTCTTTATCGTATTCACCTGGCTACTTTGATACGAGCAGAAGAATCAGATTTGAAAAGAGTTAATCTCAGGAGTGATAGAGCTAGAACAGTGGCAGCAGAACAAGAGGCATCTGGCCTGCCTGAAATGGATTTCTCTCTCAGAATCCTTGTCCTAGGGAAAACAGGAGTTGGCAAGAGTGCTACAATAAACTCTATATTTGACCAAACGATGACTGAAACCAATGCATTTCGACCTGGCACAAATCGTATTCGAGAGGTTGTGGGAACTGTGAATGGGATTCGAGTAAATATCATCGATACCCCTGGTTTTTTGCCATCATGTACTGGTAATTTCTGCAGAAATAAGAAGATTATGCTCTCTGTGAAGAAATTTATTAGAAAAAGTCCACCTGACATCGTTTTGTTCTTCGAACGCCTCGACCTCATGAGTACTAGCTACAATGACTTCTCCCTCTTGAAGCAAATAACCGAAGTATTCGGTCCTGCAGTTTGGTTTAACACCATTCTTGTCATGACACATTCTTCCCTAGCACTTCCAGAAGGACTTGATGGGAATCCCGTCAATTATGAATCATATGTGAGGCAAAGCACAGATATGGTGCAGCACTATATACACCAGGCAGTGTTTGACTCCAGAATTGAAAACCCTGTGTTGTTAGTTGAGAATCATCTTCAGTGTAGGAAAAATATCGCCGGTGAAAAGATACTTCCAAACGGACAGGTTTGGAAATCCCAGTTTTTGTTATTATGCATTTGTACTAAAGTTCTGAGTGATGTCAATATCCTCATGAAATTTCAAGACAGCATTCATCTGGGGCCCTCAAATGCCACTCACGTGCCTTCTCTGCCGCATCTCCTCTCGTCTCTTCTACGTCATCGCTCTACCATTAGTCCAAGTGGAGGGGATGTTGAAGTTGATGAGAGCTTGGTTTCATATACGCAGGAGGAAGATGAGTATGATCAATTACCTCCAATCCGAATTCTAACAAAATCTCAGTTTGAGAGATTAACAAAATCACAGAAAAAAGACTATCTTGATGAATTGGATTATCGAGAGACTCTTTATCTAAAGAAACAGTTGAAAGAAGAGTATCGTAGGCAGATGGAGGTTAAGCTTTCCAAAGAGAAAAATATGGCAAGTGGTGATAATTCTGACAGTCAGCAGGCTTCCCAAGAAGAGGCTGTTTTATTACCAGAGATGGAGGTCCCTCCAAGTTTTGGCTCTAATTGCCCTGCACACAGGTATCGTTGCCTTGTTACAAGTGATCAGTGGATTATGAGACCTGTTCTTGATTCCCATGGATGGGATAATGATGTGGGCTTGGATGGGGTTAGCTTGGAAGCTGCTATGCAGGTGAACAGGAATGTGTTTACCTCTGTCGCAGGGCAGATGAGCAAGGACAAGCAGGATTTTAGCATTCAATCTGAGTGTGCTGCAGCTTACTTAGACCACAGTGGGACCACATATTCTGTAGGTCTTGATGTTCAGTCTGCTGGTAAAGATACCATCTATACGCTTCACAGCAACACGAAGCTGAGGAAGTTGTGGCACAATGTTGCTGAAAGTGGATTCTCATTGACGTCCTTTGGGAACAAGTATTATATTGGCGGAAAGCTTGAAGACACCATATCAGTTGGGAAGAGATTGAAACTTGTGATGAATGTTGGCCAAATGATGGGTCCTGAGCAAGTGGCGTATGGTGGCAGTGTTGAAGCTACGTTGAGGGGTAGAGACTATCCTGTGAGAAATGACAATGTGAGCCTGATGATGACTATCCTGTCTTTCAATAAAGAGATGGTGTTGGGTGGAAATTTACAGTCCGAGTCCCGGCTGGGGCGAGATATGAGAGTGTCGGTTAATGCTAATATGAATAACCGTAGGATGGGTAAGATAAGCATCAAAACAAGTAGCACTGATCATTTGCAGTTCAGTATGGTTGCGGCTTTCACAATTTTCTGGGCACTTTTACGGAAGAAGGATGTTAAAAGTACAGTTCATGAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006605 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0015031 GO:0015833 GO:0033036 GO:0033365 GO:0034613 GO:0042886 GO:0045036 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0072594 GO:0072596 GO:0072598

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AIG1 TOC159_MAD

Protein Analysis

781

Amino Acids

87.61

Weight (kDa)

7.08

Isoelectric Point (pI)

45.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AIG1	PF04548	159 - 294	1.6e-20	AIG1 family
MMR_HSR1	PF01926	160 - 249	1.3e-07	50S ribosome-binding GTPase
TOC159_MAD	PF11886	506 - 770	4.5e-113	Translocase of chloroplast 159/132, membrane anchor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004955)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20300 AT5G20300 AT5G20300 AT5G20300 AT5G20300
fragaria_vesca	FvH4_1g02420 FvH4_1g02420 FvH4_1g02420 FvH4_1g02420 FvH4_1g02420 FvH4_1g02420 FvH4_1g02420 FvH4_1g02420
malus_domestica	MD02G1020900.v1.1 MD15G1164100.v1.1
prunus_persica	Prupe.7G250500_v2.0.a1 Prupe.7G250500_v2.0.a1
pyrus_communis	pycom02g01840 pycom15g14720
rosa_chinensis	RchiOBHm_Chr2g0087381
rosa_laevigata	RLG00000015861
rosa_multiflora	Rmu_sc0012811.1_g000001
rosa_roxburghii	Rroxscaffold_2G00153590
rosa_rugosa	Rorug01G0474600 Rorug01G0474700
rosa_samantha	Rh2AG028200 Rh2BG028100 Rh2CG028400 Rh2DG028200
rosa_wichuraiana	Rw2G002240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1681
AasI	GACNNNNNNGTC	1 cut(s)	2116
AatII	GACGTC	1 cut(s)	1922
Acc36I	ACCTGC	1 cut(s)	1681
AccB7I	CCANNNNNTGG	2 cut(s)	1556, 2014
AciI	CCGC	4 cut(s)	132, 1187, 1947, 2279
AclWI	GGATC	3 cut(s)	216, 239, 252
AcoI	YGGCCR	2 cut(s)	20, 2005
AcsI	RAATTY	5 cut(s)	202, 707, 1130, 1317, 2149
AcuI	CTGAAG	1 cut(s)	995
AcvI	CACGTG	1 cut(s)	1174
AcyI	GRCGYC	1 cut(s)	1919
AfaI	GTAC	4 cut(s)	661, 769, 1096, 2329
AjnI	CCWGG	4 cut(s)	354, 571, 640, 954
AleI	CACNNNNGTG	1 cut(s)	1172
Alw26I	GTCTC	4 cut(s)	1206, 1388, 1612, 2064
AlwI	GGATC	3 cut(s)	216, 239, 252
AoxI	GGCC	4 cut(s)	20, 451, 1154, 2005
ApaI	GGGCCC	1 cut(s)	1158
ApeKI	GCWGC	7 cut(s)	89, 184, 431, 940, 1682, 1772, 1775
ApoI	RAATTY	5 cut(s)	202, 707, 1130, 1317, 2149
ArsI	GACNNNNNNTTYG	2 cut(s)	2002, 2034
AspA2I	CCTAGG	1 cut(s)	490
AspLEI	GCGC	1 cut(s)	288
AspS9I	GGNCC	7 cut(s)	173, 818, 1154, 1155, 1543, 1797, 2018
AsuC2I	CCSGG	1 cut(s)	2167
AsuHPI	GGTGA	4 cut(s)	344, 1046, 1493, 1705
AsuII	TTCGAA	1 cut(s)	747
AvaII	GGWCC	5 cut(s)	173, 818, 1543, 1797, 2018
AvrII	CCTAGG	1 cut(s)	490
BaeGI	GKGCMC	2 cut(s)	1158, 2302
BaeI	ACNNNNGTAYC	2 cut(s)	1565, 1598
BalI	TGGCCA	1 cut(s)	2007
BamHI	GGATCC	1 cut(s)	244
BanII	GRGCYC	1 cut(s)	1158
BbrPI	CACGTG	1 cut(s)	1174
BbsI	GAAGAC	1 cut(s)	1965
BbvCI	CCTCAGC	1 cut(s)	1874
BbvI	GCAGC	7 cut(s)	76, 196, 443, 952, 1669, 1759, 1787
BceAI	ACGGC	1 cut(s)	314
BciT130I	CCWGG	4 cut(s)	356, 573, 642, 956
BclI	TGATCA	4 cut(s)	154, 1297, 1603, 2254
BcnI	CCSGG	1 cut(s)	2167
BcoDI	GTCTC	4 cut(s)	1206, 1388, 1612, 2064
BfaI	CTAG	4 cut(s)	420, 491, 771, 866
BfmI	CTRYAG	6 cut(s)	182, 192, 673, 822, 1773, 1809
BfuAI	ACCTGC	1 cut(s)	1681
BlnI	CCTAGG	1 cut(s)	490
BmcAI	AGTACT	1 cut(s)	769
Bme1390I	CCNGG	5 cut(s)	356, 573, 642, 956, 2167
Bme18I	GGWCC	5 cut(s)	173, 818, 1543, 1797, 2018
BmgT120I	GGNCC	7 cut(s)	173, 818, 1154, 1155, 1543, 1797, 2018
BmiI	GGNNCC	6 cut(s)	246, 1155, 1156, 1545, 1798, 2019
BmrFI	CCNGG	5 cut(s)	356, 573, 642, 956, 2167
BmrI	ACTGGG	1 cut(s)	1066
BmsI	GCATC	5 cut(s)	129, 143, 455, 1198, 2244
BmuI	ACTGGG	1 cut(s)	1066
BpiI	GAAGAC	1 cut(s)	1965
BplI	GAGNNNNNCTC	4 cut(s)	44, 76, 387, 419
BpmI	CTGGAG	1 cut(s)	955
Bpu10I	CCTNAGC	3 cut(s)	333, 1874, 2022
Bpu14I	TTCGAA	1 cut(s)	747
BpuMI	CCSGG	1 cut(s)	2167
Bsa29I	ATCGAT	1 cut(s)	633
BsaAI	YACGTR	1 cut(s)	1174
BsaBI	GATNNNNATC	3 cut(s)	891, 2111, 2234
BsaHI	GRCGYC	1 cut(s)	1919
BsaI	GGTCTC	1 cut(s)	1612
BsaJI	CCNNGG	5 cut(s)	17, 82, 490, 640, 1636
BsaXI	ACNNNNNCTCC	2 cut(s)	1230, 1260
Bse118I	RCCGGY	1 cut(s)	1031
Bse1I	ACTGG	2 cut(s)	667, 1072
Bse8I	GATNNNNATC	3 cut(s)	891, 2111, 2234
BseBI	CCWGG	4 cut(s)	356, 573, 642, 956
BseCI	ATCGAT	1 cut(s)	633
BseDI	CCNNGG	5 cut(s)	17, 82, 490, 640, 1636
BseGI	GGATG	7 cut(s)	120, 124, 1249, 1646, 1669, 2226, 2323
BseJI	GATNNNNATC	3 cut(s)	891, 2111, 2234
BseMII	CTCAG	7 cut(s)	418, 490, 1097, 1346, 1755, 1865, 2013
BseNI	ACTGG	2 cut(s)	667, 1072
BseRI	GAGGAG	2 cut(s)	281, 1184
BseSI	GKGCMC	2 cut(s)	1158, 2302
BseXI	GCAGC	7 cut(s)	76, 196, 443, 952, 1669, 1759, 1787
BseYI	CCCAGC	1 cut(s)	2170
BsgI	GTGCAG	2 cut(s)	959, 1557
BshFI	GGCC	4 cut(s)	22, 453, 1156, 2007
BshVI	ATCGAT	1 cut(s)	633
BsiSI	CCGG	2 cut(s)	1032, 2167
BslFI	GGGAC	3 cut(s)	1529, 1810, 2149
BsmAI	GTCTC	4 cut(s)	1206, 1388, 1612, 2064
BsmBI	CGTCTC	1 cut(s)	1206
BsmFI	GGGAC	3 cut(s)	1529, 1810, 2149
BsmI	GAATGC	2 cut(s)	1143, 1755
BsnI	GGCC	4 cut(s)	22, 453, 1156, 2007
Bso31I	GGTCTC	1 cut(s)	1612
Bsp119I	TTCGAA	1 cut(s)	747
Bsp120I	GGGCCC	1 cut(s)	1154
Bsp1286I	GDGCHC	2 cut(s)	1158, 2302
Bsp143I	GATC	7 cut(s)	154, 208, 244, 265, 1297, 1603, 2254
Bsp19I	CCATGG	1 cut(s)	1636
BspACI	CCGC	4 cut(s)	132, 1187, 1947, 2279
BspANI	GGCC	4 cut(s)	22, 453, 1156, 2007
BspCNI	CTCAG	7 cut(s)	417, 489, 1098, 1345, 1756, 1866, 2014
BspDI	ATCGAT	1 cut(s)	633
BspHI	TCATGA	4 cut(s)	762, 849, 1125, 2335
BspLI	GGNNCC	6 cut(s)	246, 1155, 1156, 1545, 1798, 2019
BspMAI	CTGCAG	4 cut(s)	186, 677, 826, 1777
BspMI	ACCTGC	1 cut(s)	1681
BspPI	GGATC	3 cut(s)	216, 239, 252
BspT104I	TTCGAA	1 cut(s)	747
BspTNI	GGTCTC	1 cut(s)	1612
BsrFI	RCCGGY	1 cut(s)	1031
BsrI	ACTGG	2 cut(s)	667, 1072
BssAI	RCCGGY	1 cut(s)	1031
BssECI	CCNNGG	5 cut(s)	17, 82, 490, 640, 1636
BssMI	GATC	7 cut(s)	154, 208, 244, 265, 1297, 1603, 2254
BssNI	GRCGYC	1 cut(s)	1919
BssT1I	CCWWGG	3 cut(s)	82, 490, 1636
Bst2UI	CCWGG	4 cut(s)	356, 573, 642, 956
Bst4CI	ACNGT	8 cut(s)	427, 607, 1416, 1496, 1793, 2157, 2216, 2332
Bst6I	CTCTTC	5 cut(s)	63, 1209, 1419, 1509, 1973
BstACI	GRCGYC	1 cut(s)	1919
BstBAI	YACGTR	1 cut(s)	1174
BstBI	TTCGAA	1 cut(s)	747
BstC8I	GCNNGC	3 cut(s)	189, 455, 1503
BstDEI	CTNAG	9 cut(s)	333, 404, 476, 1106, 1332, 1764, 1783, 1874, 2022
BstDSI	CCRYGG	1 cut(s)	1636
BstF5I	GGATG	7 cut(s)	120, 124, 1249, 1646, 1669, 2226, 2323
BstHHI	GCGC	1 cut(s)	288
BstKTI	GATC	7 cut(s)	157, 211, 247, 268, 1300, 1606, 2257
BstMAI	GTCTC	4 cut(s)	1206, 1388, 1612, 2064
BstMBI	GATC	7 cut(s)	154, 208, 244, 265, 1297, 1603, 2254
BstMWI	GCNNNNNNNGC	2 cut(s)	1183, 1566
BstNI	CCWGG	4 cut(s)	356, 573, 642, 956
BstNSI	RCATGY	1 cut(s)	191
BstSCI	CCNGG	5 cut(s)	354, 571, 640, 954, 2165
BstSFI	CTRYAG	6 cut(s)	182, 192, 673, 822, 1773, 1809
BstSLI	GKGCMC	2 cut(s)	1158, 2302
BstV1I	GCAGC	7 cut(s)	76, 196, 443, 952, 1669, 1759, 1787
BstV2I	GAAGAC	1 cut(s)	1965
BstX2I	RGATCY	1 cut(s)	244
BstYI	RGATCY	1 cut(s)	244
Bsu15I	ATCGAT	1 cut(s)	633
BsuRI	GGCC	4 cut(s)	22, 453, 1156, 2007
BsuTUI	ATCGAT	1 cut(s)	633
BtgI	CCRYGG	1 cut(s)	1636
BtgZI	GCGATG	1 cut(s)	1199
BtsCI	GGATG	7 cut(s)	120, 124, 1249, 1646, 1669, 2226, 2323
BtsI	GCAGTG	3 cut(s)	40, 966, 2050
BtsIMutI	CAGTG	8 cut(s)	40, 432, 966, 1019, 1613, 1798, 2050, 2250
BveI	ACCTGC	1 cut(s)	1681
Cac8I	GCNNGC	3 cut(s)	189, 455, 1503
CciI	TCATGA	4 cut(s)	762, 849, 1125, 2335
CfoI	GCGC	1 cut(s)	288
Cfr10I	RCCGGY	1 cut(s)	1031
Cfr13I	GGNCC	7 cut(s)	173, 818, 1154, 1155, 1543, 1797, 2018
ClaI	ATCGAT	1 cut(s)	633
Csp6I	GTAC	4 cut(s)	660, 768, 1095, 2328
CviAII	CATG	7 cut(s)	188, 657, 763, 850, 1126, 1637, 2336
CviQI	GTAC	4 cut(s)	660, 768, 1095, 2328
DdeI	CTNAG	9 cut(s)	333, 404, 476, 1106, 1332, 1764, 1783, 1874, 2022
DpnI	GATC	7 cut(s)	156, 210, 246, 267, 1299, 1605, 2256
DpnII	GATC	7 cut(s)	154, 208, 244, 265, 1297, 1603, 2254
DrdI	GACNNNNNNGTC	1 cut(s)	2116
DseDI	GACNNNNNNGTC	1 cut(s)	2116
EaeI	YGGCCR	2 cut(s)	20, 2005
Eam1104I	CTCTTC	5 cut(s)	63, 1209, 1419, 1509, 1973
EarI	CTCTTC	5 cut(s)	63, 1209, 1419, 1509, 1973
EciI	GGCGGA	1 cut(s)	1962
Eco130I	CCWWGG	3 cut(s)	82, 490, 1636
Eco24I	GRGCYC	1 cut(s)	1158
Eco31I	GGTCTC	1 cut(s)	1612
Eco47I	GGWCC	5 cut(s)	173, 818, 1543, 1797, 2018
Eco57I	CTGAAG	1 cut(s)	995
Eco72I	CACGTG	1 cut(s)	1174
EcoO109I	RGGNCCY	4 cut(s)	1154, 1155, 1543, 2018
EcoRI	GAATTC	1 cut(s)	1317
EcoRII	CCWGG	4 cut(s)	354, 571, 640, 954
EcoT14I	CCWWGG	3 cut(s)	82, 490, 1636
EcoT22I	ATGCAT	2 cut(s)	565, 1091
EcoT38I	GRGCYC	1 cut(s)	1158
ErhI	CCWWGG	3 cut(s)	82, 490, 1636
Esp3I	CGTCTC	1 cut(s)	1206
FaeI	CATG	7 cut(s)	191, 660, 766, 853, 1129, 1640, 2339
FalI	AAGNNNNNCTT	2 cut(s)	1356, 1388
FaqI	GGGAC	3 cut(s)	1529, 1810, 2149
FatI	CATG	7 cut(s)	187, 656, 762, 849, 1125, 1636, 2335
FauNDI	CATATG	1 cut(s)	913
FbaI	TGATCA	4 cut(s)	154, 1297, 1603, 2254
FokI	GGATG	7 cut(s)	107, 111, 1256, 1653, 1676, 2233, 2330
FriOI	GRGCYC	1 cut(s)	1158
FspBI	CTAG	4 cut(s)	420, 491, 771, 866
GlaI	GCGC	1 cut(s)	287
GsaI	CCCAGC	1 cut(s)	2174
GsuI	CTGGAG	1 cut(s)	955
HaeIII	GGCC	4 cut(s)	22, 453, 1156, 2007
HapII	CCGG	2 cut(s)	1032, 2167
HhaI	GCGC	1 cut(s)	288
Hin1I	GRCGYC	1 cut(s)	1919
Hin1II	CATG	7 cut(s)	191, 660, 766, 853, 1129, 1640, 2339
Hin6I	GCGC	1 cut(s)	286
HinP1I	GCGC	1 cut(s)	286
HindIII	AAGCTT	2 cut(s)	1451, 1952
HpaII	CCGG	2 cut(s)	1032, 2167
HphI	GGTGA	4 cut(s)	344, 1046, 1493, 1705
Hpy166II	GTNNAC	3 cut(s)	725, 1696, 1710
Hpy188I	TCNGA	7 cut(s)	385, 479, 1107, 1316, 1492, 1765, 2161
Hpy8I	GTNNAC	3 cut(s)	725, 1696, 1710
HpyAV	CCTTC	4 cut(s)	186, 872, 1188, 2308
HpyCH4III	ACNGT	8 cut(s)	427, 607, 1416, 1496, 1793, 2157, 2216, 2332
HpyCH4IV	ACGT	4 cut(s)	1173, 1210, 1919, 2057
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1183, 1566
HpyF3I	CTNAG	9 cut(s)	333, 404, 476, 1106, 1332, 1764, 1783, 1874, 2022
HpySE526I	ACGT	4 cut(s)	1173, 1210, 1919, 2057
Hsp92I	GRCGYC	1 cut(s)	1919
Hsp92II	CATG	7 cut(s)	191, 660, 766, 853, 1129, 1640, 2339
HspAI	GCGC	1 cut(s)	286
Ksp22I	TGATCA	4 cut(s)	154, 1297, 1603, 2254
Kzo9I	GATC	7 cut(s)	154, 208, 244, 265, 1297, 1603, 2254
Lsp1109I	GCAGC	7 cut(s)	76, 196, 443, 952, 1669, 1759, 1787
LweI	GCATC	5 cut(s)	129, 143, 455, 1198, 2244
MaeI	CTAG	4 cut(s)	420, 491, 771, 866
MaeII	ACGT	4 cut(s)	1173, 1210, 1919, 2057
MaeIII	GTNAC	3 cut(s)	35, 109, 1594
MalI	GATC	7 cut(s)	156, 210, 246, 267, 1299, 1605, 2256
MboI	GATC	7 cut(s)	154, 208, 244, 265, 1297, 1603, 2254
MflI	RGATCY	1 cut(s)	244
MhlI	GDGCHC	2 cut(s)	1158, 2302
MlsI	TGGCCA	1 cut(s)	2007
MluNI	TGGCCA	1 cut(s)	2007
MlyI	GAGTC	3 cut(s)	962, 1390, 2171
Mox20I	TGGCCA	1 cut(s)	2007
Mph1103I	ATGCAT	2 cut(s)	565, 1091
MscI	TGGCCA	1 cut(s)	2007
MseI	TTAA	8 cut(s)	147, 276, 399, 834, 1346, 1449, 2196, 2322
MslI	CAYNNNNRTG	2 cut(s)	932, 1172
Msp20I	TGGCCA	1 cut(s)	2007
MspI	CCGG	2 cut(s)	1032, 2167
MspR9I	CCNGG	5 cut(s)	356, 573, 642, 956, 2167
Mva1269I	GAATGC	2 cut(s)	1143, 1755
MvaI	CCWGG	4 cut(s)	356, 573, 642, 956
MwoI	GCNNNNNNNGC	2 cut(s)	1183, 1566
NciI	CCSGG	1 cut(s)	2167
NcoI	CCATGG	1 cut(s)	1636
NdeI	CATATG	1 cut(s)	913
NdeII	GATC	7 cut(s)	154, 208, 244, 265, 1297, 1603, 2254
NlaIII	CATG	7 cut(s)	191, 660, 766, 853, 1129, 1640, 2339
NlaIV	GGNNCC	6 cut(s)	246, 1155, 1156, 1545, 1798, 2019
NmuCI	GTSAC	1 cut(s)	35
NsiI	ATGCAT	2 cut(s)	565, 1091
NspI	RCATGY	1 cut(s)	191
NspV	TTCGAA	1 cut(s)	747
OliI	CACNNNNGTG	1 cut(s)	1172
PaeI	GCATGC	1 cut(s)	191
PagI	TCATGA	4 cut(s)	762, 849, 1125, 2335
PaqCI	CACCTGC	1 cut(s)	1681
PcsI	WCGNNNNNNNCGW	1 cut(s)	753
PctI	GAATGC	2 cut(s)	1143, 1755
PfeI	GAWTC	9 cut(s)	56, 380, 480, 616, 892, 908, 1003, 1631, 1908
PflMI	CCANNNNNTGG	2 cut(s)	1556, 2014
PleI	GAGTC	3 cut(s)	962, 1390, 2170
PmaCI	CACGTG	1 cut(s)	1174
PmlI	CACGTG	1 cut(s)	1174
PpsI	GAGTC	3 cut(s)	962, 1390, 2170
Ppu21I	YACGTR	1 cut(s)	1174
PpuMI	RGGWCCY	2 cut(s)	1543, 2018
Psp5II	RGGWCCY	2 cut(s)	1543, 2018
Psp6I	CCWGG	4 cut(s)	354, 571, 640, 954
PspCI	CACGTG	1 cut(s)	1174
PspFI	CCCAGC	1 cut(s)	2170
PspGI	CCWGG	4 cut(s)	354, 571, 640, 954
PspN4I	GGNNCC	6 cut(s)	246, 1155, 1156, 1545, 1798, 2019
PspOMI	GGGCCC	1 cut(s)	1154
PspPI	GGNCC	7 cut(s)	173, 818, 1154, 1155, 1543, 1797, 2018
PspPPI	RGGWCCY	2 cut(s)	1543, 2018
PstI	CTGCAG	4 cut(s)	186, 677, 826, 1777
PsuI	RGATCY	1 cut(s)	244
RsaI	GTAC	4 cut(s)	661, 769, 1096, 2329
RsaNI	GTAC	4 cut(s)	660, 768, 1095, 2328
RseI	CAYNNNNRTG	2 cut(s)	932, 1172
SaqAI	TTAA	8 cut(s)	147, 276, 399, 834, 1346, 1449, 2196, 2322
Sau3AI	GATC	7 cut(s)	154, 208, 244, 265, 1297, 1603, 2254
Sau96I	GGNCC	7 cut(s)	173, 818, 1154, 1155, 1543, 1797, 2018
ScaI	AGTACT	1 cut(s)	769
SchI	GAGTC	3 cut(s)	962, 1390, 2171
ScrFI	CCNGG	5 cut(s)	356, 573, 642, 956, 2167
SduI	GDGCHC	2 cut(s)	1158, 2302
SfaNI	GCATC	5 cut(s)	129, 143, 455, 1198, 2244
SfcI	CTRYAG	6 cut(s)	182, 192, 673, 822, 1773, 1809
SfuI	TTCGAA	1 cut(s)	747
SgrAI	CRCCGGYG	1 cut(s)	1031
SinI	GGWCC	5 cut(s)	173, 818, 1543, 1797, 2018
SmiMI	CAYNNNNRTG	2 cut(s)	932, 1172
SphI	GCATGC	1 cut(s)	191
SsiI	CCGC	4 cut(s)	132, 1187, 1947, 2279
SspMI	CTAG	4 cut(s)	420, 491, 771, 866
StyD4I	CCNGG	5 cut(s)	354, 571, 640, 954, 2165
StyI	CCWWGG	3 cut(s)	82, 490, 1636
TaaI	ACNGT	8 cut(s)	427, 607, 1416, 1496, 1793, 2157, 2216, 2332
TaiI	ACGT	4 cut(s)	1176, 1213, 1922, 2060
TaqI	TCGA	8 cut(s)	98, 568, 589, 619, 633, 747, 756, 1390
TaqII	GACCGA	1 cut(s)	806
TatI	WGTACW	4 cut(s)	659, 767, 1094, 2327
TauI	GCSGC	3 cut(s)	134, 1189, 2282
TfiI	GAWTC	9 cut(s)	56, 380, 480, 616, 892, 908, 1003, 1631, 1908
Tru1I	TTAA	8 cut(s)	147, 276, 399, 834, 1346, 1449, 2196, 2322
Tru9I	TTAA	8 cut(s)	147, 276, 399, 834, 1346, 1449, 2196, 2322
TscAI	CASTG	8 cut(s)	40, 432, 966, 1019, 1613, 1798, 2050, 2257
TseFI	GTSAC	1 cut(s)	35
TseI	GCWGC	7 cut(s)	89, 184, 431, 940, 1682, 1772, 1775
Tsp45I	GTSAC	1 cut(s)	35
TspDTI	ATGAA	9 cut(s)	81, 921, 1136, 1142, 1260, 1392, 2012, 2222, 2324
TspGWI	ACGGA	2 cut(s)	1068, 2323
TspRI	CASTG	8 cut(s)	40, 432, 966, 1019, 1613, 1798, 2050, 2257
Van91I	CCANNNNNTGG	2 cut(s)	1556, 2014
VpaK11BI	GGWCC	5 cut(s)	173, 818, 1543, 1797, 2018
XapI	RAATTY	5 cut(s)	202, 707, 1130, 1317, 2149
XceI	RCATGY	1 cut(s)	191
XmaJI	CCTAGG	1 cut(s)	490
XspI	CTAG	4 cut(s)	420, 491, 771, 866
ZraI	GACGTC	1 cut(s)	1920
ZrmI	AGTACT	1 cut(s)	769
Zsp2I	ATGCAT	2 cut(s)	565, 1091

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.