MD02G1032500.v1.1

Protein of unknown function (DUF3119)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0017568
Physical Location & Seq
Forward (+)
2551069 .. 2553117
2049 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1032500.v1.1.491

Sequence Viewer

Length: 618 bp
ATGGGCAGCACTCTCTTGTCAGCTCCTACCTTCCTGCCTCTGCAAAATAGAGAAGTGGGTGGATTTGTGAGGAGCTTCAGGAGCCCAAATTATGACAAACTCAAGTTTAATCAAAGATTAATCACAAAAACTAGTGGTGGCTCCCTTCTTGTTGCCTCAGTGTTTGAAAGGAAGGTGAAGAGCAGACAGACTGTGATTCCAGAGCCGGATTATCGAATTCCGATTGTTCTACTAGGTCTATCTGGTGGGTTGGCTTACACGAACAATCTCCTACCAGCTGCGCCCGTCGGTCTCCTTGGATTACTGTTATTGTTTCAGACTACAAGAGTTAGATTTGTTTTTGATGATGAGGCTCTGGAAGTAAAAGTAGGAGAAGAGCTTGAGAGTTCAGGTGAAAATGTCTTTGTGGGCGGAAAAAATCGTTGGAAGTACTCATCGTTCGTGAACTGGGAGCTCTGGTGGCCAAATTTCCCTATTCTGGTGTACTTTAAAGAGAAGCAAACAAAGCCTGAAGGACAAGTGCACTTCTTTCCGGTGATATTTAATGGGAAGCAACTTTACGATGTCATGTTGGAGAGAGCCGGCCCTTCCCAAACTAGTGGACCGAAAGATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3119

Protein Analysis

206

Amino Acids

23.06

Weight (kDa)

9.42

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3119 PF11317 66 - 193 1.5e-34 Protein of unknown function (DUF3119)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017568)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g03270
malus_domestica MD02G1032500.v1.1
prunus_persica Prupe.7G243000_v2.0.a1 Prupe.7G243000_v2.0.a1
pyrus_communis pycom02g02670
rosa_chinensis RchiOBHm_Chr2g0088411
rosa_laevigata RLG00000015951
rosa_multiflora Rmu_sc0004235.1_g000002
rosa_roxburghii Rroxscaffold_2G00152690
rosa_rugosa Rorug01G0483900
rosa_samantha Rh2DG038500
rosa_wichuraiana Rw2G003050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 411
AcoI YGGCCR 1 cut(s) 461
AcsI RAATTY 2 cut(s) 216, 466
AcuI CTGAAG 2 cut(s) 61, 531
AfaI GTAC 2 cut(s) 431, 485
AfiI CCNNNNNNNGG 1 cut(s) 478
AgsI TTSAA 1 cut(s) 167
AhlI ACTAGT 2 cut(s) 131, 596
AjuI GAANNNNNNNTTGG 2 cut(s) 406, 438
AluBI AGCT 5 cut(s) 23, 75, 278, 379, 454
AluI AGCT 5 cut(s) 23, 75, 278, 379, 454
Alw21I GWGCWC 2 cut(s) 456, 525
Alw26I GTCTC 1 cut(s) 296
Alw44I GTGCAC 1 cut(s) 521
AoxI GGCC 2 cut(s) 461, 583
ApaLI GTGCAC 1 cut(s) 521
ApeKI GCWGC 2 cut(s) 6, 278
ApoI RAATTY 2 cut(s) 216, 466
AseI ATTAAT 1 cut(s) 119
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 2 cut(s) 584, 602
AsuHPI GGTGA 3 cut(s) 187, 404, 547
AvaII GGWCC 1 cut(s) 602
BaeGI GKGCMC 1 cut(s) 525
BalI TGGCCA 1 cut(s) 463
BanII GRGCYC 2 cut(s) 86, 456
BarI GAAGNNNNNNTAC 2 cut(s) 542, 574
Bbv12I GWGCWC 2 cut(s) 456, 525
BbvI GCAGC 2 cut(s) 18, 265
BcoDI GTCTC 1 cut(s) 296
BcuI ACTAGT 2 cut(s) 131, 596
BfaI CTAG 3 cut(s) 132, 233, 597
BisI GCNGC 2 cut(s) 7, 279
BlsI GCNGC 2 cut(s) 8, 280
BmcAI AGTACT 1 cut(s) 431
Bme18I GGWCC 1 cut(s) 602
BmgT120I GGNCC 2 cut(s) 584, 602
BmiI GGNNCC 2 cut(s) 83, 142
BmrI ACTGGG 1 cut(s) 457
BmuI ACTGGG 1 cut(s) 457
BpuEI CTTGAG 2 cut(s) 86, 401
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 295
BsaWI WCCGGW 1 cut(s) 532
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bsc4I CCNNNNNNNGG 1 cut(s) 478
Bse118I RCCGGY 1 cut(s) 581
Bse1I ACTGG 1 cut(s) 452
BseDI CCNNGG 1 cut(s) 295
BseLI CCNNNNNNNGG 1 cut(s) 478
BseMII CTCAG 1 cut(s) 171
BseNI ACTGG 1 cut(s) 452
BseRI GAGGAG 1 cut(s) 85
BseSI GKGCMC 1 cut(s) 525
BseXI GCAGC 2 cut(s) 18, 265
BshFI GGCC 2 cut(s) 463, 585
BsiHKAI GWGCWC 2 cut(s) 456, 525
BsiSI CCGG 3 cut(s) 206, 533, 582
BslI CCNNNNNNNGG 1 cut(s) 478
BsmAI GTCTC 1 cut(s) 296
BsnI GGCC 2 cut(s) 463, 585
Bso31I GGTCTC 1 cut(s) 296
Bsp1286I GDGCHC 3 cut(s) 86, 456, 525
BspACI CCGC 1 cut(s) 411
BspANI GGCC 2 cut(s) 463, 585
BspCNI CTCAG 1 cut(s) 170
BspLI GGNNCC 2 cut(s) 83, 142
BspQI GCTCTTC 2 cut(s) 173, 369
BspTNI GGTCTC 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 581
BsrI ACTGG 1 cut(s) 452
BssAI RCCGGY 1 cut(s) 581
BssECI CCNNGG 1 cut(s) 295
BssT1I CCWWGG 1 cut(s) 295
Bst4CI ACNGT 2 cut(s) 193, 306
Bst6I CTCTTC 2 cut(s) 173, 369
BstC8I GCNNGC 1 cut(s) 583
BstDEI CTNAG 1 cut(s) 157
BstHHI GCGC 1 cut(s) 283
BstMAI GTCTC 1 cut(s) 296
BstMWI GCNNNNNNNGC 3 cut(s) 81, 460, 505
BstSLI GKGCMC 1 cut(s) 525
BstV1I GCAGC 2 cut(s) 18, 265
BstXI CCANNNNNNTGG 1 cut(s) 599
BsuRI GGCC 2 cut(s) 463, 585
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 1 cut(s) 583
CfoI GCGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 581
Cfr13I GGNCC 2 cut(s) 584, 602
Csp6I GTAC 2 cut(s) 430, 484
CviAII CATG 1 cut(s) 568
CviQI GTAC 2 cut(s) 430, 484
DdeI CTNAG 1 cut(s) 157
DraI TTTAAA 1 cut(s) 490
EaeI YGGCCR 1 cut(s) 461
Eam1104I CTCTTC 2 cut(s) 173, 369
EarI CTCTTC 2 cut(s) 173, 369
EciI GGCGGA 1 cut(s) 426
Ecl136II GAGCTC 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 295
Eco24I GRGCYC 2 cut(s) 86, 456
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 602
Eco53kI GAGCTC 1 cut(s) 454
Eco57I CTGAAG 2 cut(s) 61, 531
EcoICRI GAGCTC 1 cut(s) 454
EcoRI GAATTC 1 cut(s) 216
EcoT14I CCWWGG 1 cut(s) 295
EcoT38I GRGCYC 2 cut(s) 86, 456
ErhI CCWWGG 1 cut(s) 295
FaeI CATG 1 cut(s) 571
FaiI YATR 2 cut(s) 93, 569
FatI CATG 1 cut(s) 567
Fnu4HI GCNGC 2 cut(s) 7, 279
FriOI GRGCYC 2 cut(s) 86, 456
Fsp4HI GCNGC 2 cut(s) 7, 279
FspBI CTAG 3 cut(s) 132, 233, 597
GlaI GCGC 1 cut(s) 282
GluI GCNGC 2 cut(s) 7, 279
HaeIII GGCC 2 cut(s) 463, 585
HapII CCGG 3 cut(s) 206, 533, 582
HhaI GCGC 1 cut(s) 283
Hin1II CATG 1 cut(s) 571
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HinfI GANTC 2 cut(s) 196, 611
HpaII CCGG 3 cut(s) 206, 533, 582
HphI GGTGA 3 cut(s) 187, 404, 547
Hpy166II GTNNAC 4 cut(s) 445, 484, 523, 602
Hpy188I TCNGA 2 cut(s) 222, 318
Hpy188III TCNNGA 5 cut(s) 79, 200, 356, 442, 615
Hpy8I GTNNAC 4 cut(s) 445, 484, 523, 602
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 5 cut(s) 40, 155, 166, 506, 597
HpyCH4III ACNGT 2 cut(s) 193, 306
HpyCH4V TGCA 2 cut(s) 43, 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 460, 505
HpyF3I CTNAG 1 cut(s) 157
Hsp92II CATG 1 cut(s) 571
HspAI GCGC 1 cut(s) 281
KroI GCCGGC 1 cut(s) 581
KroNI GCCGGC 1 cut(s) 583
LguI GCTCTTC 2 cut(s) 173, 369
LmnI GCTCC 5 cut(s) 28, 72, 81, 146, 451
Lsp1109I GCAGC 2 cut(s) 18, 265
MaeI CTAG 3 cut(s) 132, 233, 597
MboII GAAGA 2 cut(s) 190, 386
MhlI GDGCHC 3 cut(s) 86, 456, 525
MlsI TGGCCA 1 cut(s) 463
MluCI AATT 3 cut(s) 88, 216, 466
MluNI TGGCCA 1 cut(s) 463
MmeI TCCRAC 2 cut(s) 404, 552
MnlI CCTC 4 cut(s) 48, 63, 166, 343
Mox20I TGGCCA 1 cut(s) 463
MroNI GCCGGC 1 cut(s) 581
MscI TGGCCA 1 cut(s) 463
MseI TTAA 4 cut(s) 108, 119, 489, 543
Msp20I TGGCCA 1 cut(s) 463
MspA1I CMGCKG 1 cut(s) 278
MspI CCGG 3 cut(s) 206, 533, 582
MwoI GCNNNNNNNGC 3 cut(s) 81, 460, 505
NaeI GCCGGC 1 cut(s) 583
NgoMIV GCCGGC 1 cut(s) 581
NlaIII CATG 1 cut(s) 571
NlaIV GGNNCC 2 cut(s) 83, 142
PciSI GCTCTTC 2 cut(s) 173, 369
PdiI GCCGGC 1 cut(s) 583
PfeI GAWTC 2 cut(s) 196, 611
PkrI GCNGC 2 cut(s) 8, 280
PshBI ATTAAT 1 cut(s) 119
Psp124BI GAGCTC 1 cut(s) 456
PspN4I GGNNCC 2 cut(s) 83, 142
PspPI GGNCC 2 cut(s) 584, 602
PsrI GAACNNNNNNTAC 2 cut(s) 422, 454
PvuII CAGCTG 1 cut(s) 278
RsaI GTAC 2 cut(s) 431, 485
RsaNI GTAC 2 cut(s) 430, 484
SacI GAGCTC 1 cut(s) 456
SapI GCTCTTC 2 cut(s) 173, 369
SaqAI TTAA 4 cut(s) 108, 119, 489, 543
SatI GCNGC 2 cut(s) 7, 279
Sau96I GGNCC 2 cut(s) 584, 602
ScaI AGTACT 1 cut(s) 431
SduI GDGCHC 3 cut(s) 86, 456, 525
SetI ASST 9 cut(s) 25, 32, 77, 177, 238, 280, 381, 394, 456
SinI GGWCC 1 cut(s) 602
SmlI CTYRAG 2 cut(s) 101, 380
SmoI CTYRAG 2 cut(s) 101, 380
SpeI ACTAGT 2 cut(s) 131, 596
Sse9I AATT 3 cut(s) 88, 216, 466
SsiI CCGC 1 cut(s) 411
SspMI CTAG 3 cut(s) 132, 233, 597
SstI GAGCTC 1 cut(s) 456
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 2 cut(s) 193, 306
TaqI TCGA 1 cut(s) 214
TaqII GACCGA 1 cut(s) 278
TasI AATT 3 cut(s) 88, 216, 466
TatI WGTACW 2 cut(s) 429, 483
TfiI GAWTC 2 cut(s) 196, 611
Tru1I TTAA 4 cut(s) 108, 119, 489, 543
Tru9I TTAA 4 cut(s) 108, 119, 489, 543
TscAI CASTG 1 cut(s) 165
TseI GCWGC 2 cut(s) 6, 278
TspRI CASTG 1 cut(s) 165
VneI GTGCAC 1 cut(s) 521
VpaK11BI GGWCC 1 cut(s) 602
VspI ATTAAT 1 cut(s) 119
XapI RAATTY 2 cut(s) 216, 466
XspI CTAG 3 cut(s) 132, 233, 597
ZrmI AGTACT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.