Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0010295

Physical Location & Seq

Genomic Coordinates Reverse (-)

2660267 .. 2663491

Gene Structure 3225 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1034800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1911 bp

ATGGACGGAAAAATGGAAATCTCGCTCGACAAGCTTCCCATCAAGCGCTTGGAAGTCATTGAAGAAAACGGCCTCGAACGGTTTCCTCCTGATGTAGGCTACGACGAGAAGCGGCAGAGTTTGATTCGGAGAATCGATTTCGCGTGGGCCGTGGAGAAGGACGAGAACAAGAAGCAGAAGAAGAGCTCGAAAGAGAGCGCCGCAACGCAGTGGCAATGGCAGAGCATTGGTGGAGAACTTGCAGCTGGCGCACCAGGAGCTCTCCGTCATTATCGATCTCATCAACACCGTAAGTCTTTCAATTCTCGGGTGGAAACCAAAGATGCTGTAACGGTGGCTAGCATGACGCGTCCGAAGCCGCTTCCTAATGAAGCTCTGTCTGATCTTGCTGTGTCTGCAGCAACCAAGCTTCAATGCTTCAGGGTAATGCTGTTCTCGCCGGGCTCCAGATTTCGTAAATTTTGTAAATTTGGTAACTTTTTCATCTTGGAAAGTACTTTAAACAGTCAGCTAGATTTTACGGTGCTCCAATCAGATAGACTGCAGCAGAATTGGAAAGTTAAGCGGCTGCGTATGGCAGCTTCAACTTCTGGAAATGAAGGCTTTACCATTGATCTGTTTGACAACTCATTATATGATCCAGCTGCTATATTTTGGCCGTCTGCTCTGTCTACAGTTCATGTTGAACATGACTCGGCTGGCATGCTGGCAATAAATGCTGAGAAAAAATCTGTAAGTGACGATGAGTGTGTTAAAGAAACACATTTGCTTCTTCGCGAAGTTCATCAAGCAATATTCATTGAGCAGGTGTTTAATTTGGTAAATCGCGAAGCTTTCAACCAAACTTTAGGAGTCAATGTAACTGGAATACAAGAAAACTATTTACAACTGAATATAGGTGAAGGAACCTCTGTGTTCATATCACTTATCCCGTCTAAAGGTGAACAAACGGGTGACAGTCCAAGCACCCAAAACATAGAGAATGCAATTTTACCTTTGGACACATTGGATGGCTTGCAGTTCCCAGAGGATGATAAACCGGATACCCCAAAGAAAATGTCAGTAATTCATGATCAAATTAGTTGTGAAATATATCGGCAACAGATTTTTCACCAGCATGTATTTGTCAAAGCAAAAAATGTACCAACATCAACTCGAGTATCTGGTCAACCAGCCAAAGAAGGGTCCAGACTTCTTGGCCATTTCTGCATGTCATTGGCTCATCGAATCTTTTCAATCAAAGTTCTTACAGAGATGGAAAATGTGGTAAAGTTAAAGGGGCACCATATCTGCAGCTGTATCTCAACCCACTTGGCATTCGCGGTCATCATCATGGATGCTCTCTGTGAAGATTCCTTATCCATTCGACATGCATGTCAAACCCAAGCATCTGACATGCGTCACGTGGGGAACGTTGCCAAGTCTCAGTTTCATACTAAGGTGGTTGTAACAGATGATTGCATCAATGTAGAAGGGGAAGGTGCTCCGAATATGGTTGGCCTGTTCAATGGGAATTTTGAGGAAATTTGTTCAATGAACAGATACAACTGCAACTTGGCAGATCTTCCTGTGATAATTTTGCAGCAGGTTGCAGGTCAAGTTATTTACTGGCTTCACGAAGAAGCTCTTGCGGTAGGAATAAAGGCAAACCGAGACTTTCTTTGTTTGTCACTTGAGCTGGATCAGGGCGAAACACTCAGCGTGGTGGCACATGTGGACCCAGAAGACAGTGGAGGATGCATCTCATGGTGGCTGGTCATAGACGGTGGGTTTATAGAGGAACGAAAACTCAGTATGGACACTTCCGATGCTGCGTCAGAAACTAGGAAGTTTTTAGGTCATCTATCCCTGGAAGTGTTGTATTCAACACTGATGTACTTGGTTAGCCTGTGCAACGGAGGCGGTAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016592 GO:0019222 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901698 GO:1901699

KEGG Pathways

Metabolic & Signaling

ko04919 map04919

Pfam Domains

Protein Families

Med17

Protein Analysis

637

Amino Acids

70.94

Weight (kDa)

5.75

Isoelectric Point (pI)

41.37

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010295)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20170
fragaria_vesca	FvH4_5g23630 FvH4_5g23630 FvH4_5g23630 FvH4_5g23630
malus_domestica	MD02G1034800.v1.1 MD15G1175600.v1.1
prunus_persica	Prupe.7G240500_v2.0.a1
pyrus_communis	pycom02g02840 pycom15g15710
rosa_chinensis	RchiOBHm_Chr7g0212931
rosa_laevigata	RLG00000002843
rosa_multiflora	Rmu_co8301145.1_g000001 Rmu_sc0001684.1_g000012
rosa_roxburghii	Rroxscaffold_3G00246410
rosa_rugosa	Rorug07G0138300
rosa_samantha	Rh7AG269500 Rh7BG265000 Rh7CG288700 Rh7DG277600
rosa_wichuraiana	Rw7G023410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	796
AasI	GACNNNNNNGTC	1 cut(s)	1374
Acc36I	ACCTGC	3 cut(s)	796, 1576, 1583
AccB1I	GGYRCC	1 cut(s)	1281
AccI	GTMKAC	1 cut(s)	671
AccII	CGCG	5 cut(s)	143, 349, 777, 828, 1322
AciI	CCGC	7 cut(s)	112, 201, 359, 565, 1322, 1631, 1902
AclI	AACGTT	1 cut(s)	1413
AclWI	GGATC	2 cut(s)	632, 1689
AcoI	YGGCCR	2 cut(s)	656, 1198
AcsI	RAATTY	4 cut(s)	458, 467, 1513, 1524
AcuI	CTGAAG	1 cut(s)	403
AcvI	CACGTG	1 cut(s)	1405
AfaI	GTAC	3 cut(s)	496, 1143, 1877
AfeI	AGCGCT	1 cut(s)	47
AfiI	CCNNNNNNNGG	3 cut(s)	95, 938, 1182
AflIII	ACRYGT	2 cut(s)	347, 1711
AjnI	CCWGG	2 cut(s)	253, 1848
Alw21I	GWGCWC	4 cut(s)	188, 262, 528, 1486
Alw26I	GTCTC	2 cut(s)	1428, 1647
AlwI	GGATC	2 cut(s)	632, 1689
Ama87I	CYCGRG	2 cut(s)	306, 1155
Aor51HI	AGCGCT	1 cut(s)	47
AoxI	GGCC	5 cut(s)	70, 147, 656, 1198, 1498
ApeKI	GCWGC	9 cut(s)	242, 398, 544, 568, 578, 644, 1293, 1582, 1811
ApoI	RAATTY	4 cut(s)	458, 467, 1513, 1524
ArsI	GACNNNNNNTTYG	2 cut(s)	1043, 1075
Asp700I	GAANNNNTTC	2 cut(s)	81, 1231
AspLEI	GCGC	3 cut(s)	48, 200, 251
AspS9I	GGNCC	3 cut(s)	147, 1185, 1717
AsuC2I	CCSGG	1 cut(s)	441
AsuHPI	GGTGA	4 cut(s)	911, 953, 965, 1103
AsuNHI	GCTAGC	1 cut(s)	338
AvaI	CYCGRG	2 cut(s)	306, 1155
AvaII	GGWCC	2 cut(s)	1185, 1717
BaeGI	GKGCMC	1 cut(s)	1284
BaeI	ACNNNNGTAYC	2 cut(s)	1143, 1176
BalI	TGGCCA	1 cut(s)	1200
BanI	GGYRCC	1 cut(s)	1281
BanII	GRGCYC	3 cut(s)	188, 262, 446
BarI	GAAGNNNNNNTAC	4 cut(s)	565, 597, 1786, 1818
BbrPI	CACGTG	1 cut(s)	1405
BbsI	GAAGAC	1 cut(s)	1731
Bbv12I	GWGCWC	4 cut(s)	188, 262, 528, 1486
BbvI	GCAGC	9 cut(s)	254, 410, 555, 556, 590, 631, 1305, 1594, 1798
BccI	CCATC	3 cut(s)	47, 1004, 1249
BceAI	ACGGC	3 cut(s)	85, 134, 643
BciT130I	CCWGG	2 cut(s)	255, 1850
BciVI	GTATCC	1 cut(s)	1036
BclI	TGATCA	1 cut(s)	1072
BcnI	CCSGG	1 cut(s)	441
BcoDI	GTCTC	2 cut(s)	1428, 1647
BfaI	CTAG	3 cut(s)	339, 512, 1824
BfmI	CTRYAG	4 cut(s)	396, 542, 672, 1291
BfoI	RGCGCY	2 cut(s)	49, 201
BfuAI	ACCTGC	3 cut(s)	796, 1576, 1583
BfuI	GTATCC	1 cut(s)	1036
BglII	AGATCT	1 cut(s)	1561
BmcAI	AGTACT	1 cut(s)	496
Bme1390I	CCNGG	3 cut(s)	255, 441, 1850
Bme18I	GGWCC	2 cut(s)	1185, 1717
BmeT110I	CYCGRG	2 cut(s)	306, 1155
BmgT120I	GGNCC	3 cut(s)	147, 1185, 1717
BmiI	GGNNCC	5 cut(s)	445, 907, 1186, 1283, 1719
BmrFI	CCNGG	3 cut(s)	255, 441, 1850
BmsI	GCATC	7 cut(s)	313, 1327, 1397, 1470, 1727, 1749, 1798
BmtI	GCTAGC	1 cut(s)	342
BoxI	GACNNNNGTC	1 cut(s)	1398
BpiI	GAAGAC	1 cut(s)	1731
BpmI	CTGGAG	1 cut(s)	430
BpuEI	CTTGAG	1 cut(s)	1694
BpuMI	CCSGG	1 cut(s)	441
Bsa29I	ATCGAT	2 cut(s)	135, 274
BsaAI	YACGTR	1 cut(s)	1405
BsaJI	CCNNGG	2 cut(s)	150, 1848
BsaWI	WCCGGW	1 cut(s)	1039
BsaXI	ACNNNNNCTCC	2 cut(s)	843, 873
Bsc4I	CCNNNNNNNGG	3 cut(s)	95, 938, 1182
Bse1I	ACTGG	2 cut(s)	868, 1613
Bse3DI	GCAATG	1 cut(s)	221
BseBI	CCWGG	2 cut(s)	255, 1850
BseCI	ATCGAT	2 cut(s)	135, 274
BseDI	CCNNGG	2 cut(s)	150, 1848
BseGI	GGATG	4 cut(s)	1015, 1036, 1342, 1742
BseLI	CCNNNNNNNGG	3 cut(s)	95, 938, 1182
BseMI	GCAATG	1 cut(s)	221
BseMII	CTCAG	4 cut(s)	711, 1439, 1711, 1804
BseNI	ACTGG	2 cut(s)	868, 1613
BseSI	GKGCMC	1 cut(s)	1284
BseXI	GCAGC	9 cut(s)	254, 410, 555, 556, 590, 631, 1305, 1594, 1798
Bsh1236I	CGCG	5 cut(s)	143, 349, 777, 828, 1322
BshFI	GGCC	5 cut(s)	72, 149, 658, 1200, 1500
BshNI	GGYRCC	1 cut(s)	1281
BshVI	ATCGAT	2 cut(s)	135, 274
BsiHKAI	GWGCWC	4 cut(s)	188, 262, 528, 1486
BsiHKCI	CYCGRG	2 cut(s)	306, 1155
BsiSI	CCGG	2 cut(s)	440, 1040
BslI	CCNNNNNNNGG	3 cut(s)	95, 938, 1182
BsmAI	GTCTC	2 cut(s)	1428, 1647
BsmI	GAATGC	2 cut(s)	988, 1316
BsnI	GGCC	5 cut(s)	72, 149, 658, 1200, 1500
BsoBI	CYCGRG	2 cut(s)	306, 1155
Bsp1286I	GDGCHC	6 cut(s)	188, 262, 446, 528, 1284, 1486
Bsp143I	GATC	7 cut(s)	275, 382, 613, 637, 1072, 1561, 1681
Bsp68I	TCGCGA	2 cut(s)	777, 828
BspACI	CCGC	7 cut(s)	112, 201, 359, 565, 1322, 1631, 1902
BspANI	GGCC	5 cut(s)	72, 149, 658, 1200, 1500
BspCNI	CTCAG	4 cut(s)	712, 1438, 1710, 1803
BspDI	ATCGAT	2 cut(s)	135, 274
BspFNI	CGCG	5 cut(s)	143, 349, 777, 828, 1322
BspHI	TCATGA	1 cut(s)	1069
BspLI	GGNNCC	5 cut(s)	445, 907, 1186, 1283, 1719
BspMAI	CTGCAG	3 cut(s)	400, 546, 1295
BspMI	ACCTGC	3 cut(s)	796, 1576, 1583
BspOI	GCTAGC	1 cut(s)	342
BspPI	GGATC	2 cut(s)	632, 1689
BspQI	GCTCTTC	1 cut(s)	176
BspT107I	GGYRCC	1 cut(s)	1281
BsrDI	GCAATG	1 cut(s)	221
BsrI	ACTGG	2 cut(s)	868, 1613
BssECI	CCNNGG	2 cut(s)	150, 1848
BssMI	GATC	7 cut(s)	275, 382, 613, 637, 1072, 1561, 1681
Bst2UI	CCWGG	2 cut(s)	255, 1850
Bst4CI	ACNGT	9 cut(s)	81, 290, 334, 506, 523, 676, 959, 1730, 1766
Bst6I	CTCTTC	1 cut(s)	176
BstAPI	GCANNNNNTGC	1 cut(s)	716
BstBAI	YACGTR	1 cut(s)	1405
BstC8I	GCNNGC	6 cut(s)	247, 340, 700, 704, 708, 1016
BstDEI	CTNAG	5 cut(s)	720, 1425, 1437, 1697, 1790
BstDSI	CCRYGG	1 cut(s)	150
BstENI	CCTNNNNNAGG	1 cut(s)	93
BstF5I	GGATG	4 cut(s)	1015, 1036, 1342, 1742
BstFNI	CGCG	5 cut(s)	143, 349, 777, 828, 1322
BstH2I	RGCGCY	2 cut(s)	49, 201
BstHHI	GCGC	3 cut(s)	48, 200, 251
BstKTI	GATC	7 cut(s)	278, 385, 616, 640, 1075, 1564, 1684
BstMAI	GTCTC	2 cut(s)	1428, 1647
BstMBI	GATC	7 cut(s)	275, 382, 613, 637, 1072, 1561, 1681
BstMWI	GCNNNNNNNGC	9 cut(s)	31, 248, 257, 355, 395, 436, 716, 1206, 1899
BstNI	CCWGG	2 cut(s)	255, 1850
BstNSI	RCATGY	7 cut(s)	706, 1121, 1213, 1373, 1377, 1399, 1715
BstPAI	GACNNNNGTC	1 cut(s)	1398
BstSCI	CCNGG	3 cut(s)	253, 439, 1848
BstSFI	CTRYAG	4 cut(s)	396, 542, 672, 1291
BstSLI	GKGCMC	1 cut(s)	1284
BstUI	CGCG	5 cut(s)	143, 349, 777, 828, 1322
BstV1I	GCAGC	9 cut(s)	254, 410, 555, 556, 590, 631, 1305, 1594, 1798
BstV2I	GAAGAC	1 cut(s)	1731
BstX2I	RGATCY	1 cut(s)	1561
BstYI	RGATCY	1 cut(s)	1561
Bsu15I	ATCGAT	2 cut(s)	135, 274
BsuI	GTATCC	1 cut(s)	1036
BsuRI	GGCC	5 cut(s)	72, 149, 658, 1200, 1500
BsuTUI	ATCGAT	2 cut(s)	135, 274
BtgI	CCRYGG	1 cut(s)	150
BtsCI	GGATG	4 cut(s)	1015, 1036, 1342, 1742
BtsI	GCAGTG	1 cut(s)	215
BtsIMutI	CAGTG	3 cut(s)	215, 1735, 1868
BtuMI	TCGCGA	2 cut(s)	777, 828
BveI	ACCTGC	3 cut(s)	796, 1576, 1583
Cac8I	GCNNGC	6 cut(s)	247, 340, 700, 704, 708, 1016
CciI	TCATGA	1 cut(s)	1069
CfoI	GCGC	3 cut(s)	48, 200, 251
Cfr13I	GGNCC	3 cut(s)	147, 1185, 1717
ClaI	ATCGAT	2 cut(s)	135, 274
CseI	GACGC	4 cut(s)	338, 355, 1388, 1803
Csp6I	GTAC	3 cut(s)	495, 1142, 1876
CviQI	GTAC	3 cut(s)	495, 1142, 1876
DdeI	CTNAG	5 cut(s)	720, 1425, 1437, 1697, 1790
DpnI	GATC	7 cut(s)	277, 384, 615, 639, 1074, 1563, 1683
DpnII	GATC	7 cut(s)	275, 382, 613, 637, 1072, 1561, 1681
DraI	TTTAAA	1 cut(s)	501
DrdI	GACNNNNNNGTC	1 cut(s)	1374
DseDI	GACNNNNNNGTC	1 cut(s)	1374
EaeI	YGGCCR	2 cut(s)	656, 1198
Eam1104I	CTCTTC	1 cut(s)	176
EarI	CTCTTC	1 cut(s)	176
Ecl136II	GAGCTC	2 cut(s)	186, 260
Eco24I	GRGCYC	3 cut(s)	188, 262, 446
Eco47I	GGWCC	2 cut(s)	1185, 1717
Eco47III	AGCGCT	1 cut(s)	47
Eco53kI	GAGCTC	2 cut(s)	186, 260
Eco57I	CTGAAG	1 cut(s)	403
Eco72I	CACGTG	1 cut(s)	1405
Eco88I	CYCGRG	2 cut(s)	306, 1155
EcoICRI	GAGCTC	2 cut(s)	186, 260
EcoNI	CCTNNNNNAGG	1 cut(s)	93
EcoRII	CCWGG	2 cut(s)	253, 1848
EcoT22I	ATGCAT	2 cut(s)	1375, 1742
EcoT38I	GRGCYC	3 cut(s)	188, 262, 446
FalI	AAGNNNNNCTT	2 cut(s)	1611, 1643
FbaI	TGATCA	1 cut(s)	1072
FblI	GTMKAC	1 cut(s)	671
FokI	GGATG	4 cut(s)	1022, 1043, 1349, 1749
FriOI	GRGCYC	3 cut(s)	188, 262, 446
FspBI	CTAG	3 cut(s)	339, 512, 1824
GlaI	GCGC	3 cut(s)	47, 199, 250
GsuI	CTGGAG	1 cut(s)	430
HaeII	RGCGCY	2 cut(s)	49, 201
HaeIII	GGCC	5 cut(s)	72, 149, 658, 1200, 1500
HapII	CCGG	2 cut(s)	440, 1040
HgaI	GACGC	4 cut(s)	338, 355, 1388, 1803
HhaI	GCGC	3 cut(s)	48, 200, 251
Hin6I	GCGC	3 cut(s)	46, 198, 249
HinP1I	GCGC	3 cut(s)	46, 198, 249
HincII	GTYRAC	1 cut(s)	1169
HindII	GTYRAC	1 cut(s)	1169
HindIII	AAGCTT	3 cut(s)	32, 407, 831
HinfI	GANTC	6 cut(s)	124, 132, 692, 852, 1227, 1352
HpaII	CCGG	2 cut(s)	440, 1040
HphI	GGTGA	4 cut(s)	911, 953, 965, 1103
Hpy166II	GTNNAC	4 cut(s)	672, 944, 1169, 1717
Hpy188I	TCNGA	8 cut(s)	129, 354, 382, 535, 1393, 1488, 1807, 1819
Hpy188III	TCNNGA	8 cut(s)	89, 447, 591, 776, 827, 1070, 1188, 1616
Hpy8I	GTNNAC	4 cut(s)	672, 944, 1169, 1717
Hpy99I	CGWCG	1 cut(s)	107
HpyAV	CCTTC	6 cut(s)	151, 593, 896, 1175, 1466, 1472
HpyCH4III	ACNGT	9 cut(s)	81, 290, 334, 506, 523, 676, 959, 1730, 1766
HpyCH4IV	ACGT	2 cut(s)	1404, 1413
HpyF10VI	GCNNNNNNNGC	9 cut(s)	31, 248, 257, 355, 395, 436, 716, 1206, 1899
HpyF3I	CTNAG	5 cut(s)	720, 1425, 1437, 1697, 1790
HpySE526I	ACGT	2 cut(s)	1404, 1413
HspAI	GCGC	3 cut(s)	46, 198, 249
Ksp22I	TGATCA	1 cut(s)	1072
Kzo9I	GATC	7 cut(s)	275, 382, 613, 637, 1072, 1561, 1681
LguI	GCTCTTC	1 cut(s)	176
LmnI	GCTCC	4 cut(s)	257, 449, 531, 1489
Lsp1109I	GCAGC	9 cut(s)	254, 410, 555, 556, 590, 631, 1305, 1594, 1798
LweI	GCATC	7 cut(s)	313, 1327, 1397, 1470, 1727, 1749, 1798
MaeI	CTAG	3 cut(s)	339, 512, 1824
MaeII	ACGT	2 cut(s)	1404, 1413
MaeIII	GTNAC	8 cut(s)	328, 473, 737, 859, 953, 1400, 1447, 1668
MalI	GATC	7 cut(s)	277, 384, 615, 639, 1074, 1563, 1683
MboI	GATC	7 cut(s)	275, 382, 613, 637, 1072, 1561, 1681
MboII	GAAGA	8 cut(s)	74, 190, 193, 764, 1361, 1556, 1631, 1736
MflI	RGATCY	1 cut(s)	1561
MhlI	GDGCHC	6 cut(s)	188, 262, 446, 528, 1284, 1486
MlsI	TGGCCA	1 cut(s)	1200
MluI	ACGCGT	1 cut(s)	347
MluNI	TGGCCA	1 cut(s)	1200
MlyI	GAGTC	2 cut(s)	686, 861
MnlI	CCTC	8 cut(s)	83, 96, 919, 1021, 1513, 1727, 1771, 1892
Mox20I	TGGCCA	1 cut(s)	1200
Mph1103I	ATGCAT	2 cut(s)	1375, 1742
MroXI	GAANNNNTTC	2 cut(s)	81, 1231
MscI	TGGCCA	1 cut(s)	1200
MseI	TTAA	5 cut(s)	500, 561, 753, 813, 1274
MslI	CAYNNNNRTG	2 cut(s)	1116, 1331
Msp20I	TGGCCA	1 cut(s)	1200
MspA1I	CMGCKG	3 cut(s)	245, 644, 1296
MspI	CCGG	2 cut(s)	440, 1040
MspR9I	CCNGG	3 cut(s)	255, 441, 1850
Mva1269I	GAATGC	2 cut(s)	988, 1316
MvaI	CCWGG	2 cut(s)	255, 1850
MvnI	CGCG	5 cut(s)	143, 349, 777, 828, 1322
MwoI	GCNNNNNNNGC	9 cut(s)	31, 248, 257, 355, 395, 436, 716, 1206, 1899
NciI	CCSGG	1 cut(s)	441
NdeII	GATC	7 cut(s)	275, 382, 613, 637, 1072, 1561, 1681
NheI	GCTAGC	1 cut(s)	338
NlaIV	GGNNCC	5 cut(s)	445, 907, 1186, 1283, 1719
NmeAIII	GCCGAG	1 cut(s)	674
NmuCI	GTSAC	4 cut(s)	737, 953, 1400, 1668
NruI	TCGCGA	2 cut(s)	777, 828
NsiI	ATGCAT	2 cut(s)	1375, 1742
NspI	RCATGY	7 cut(s)	706, 1121, 1213, 1373, 1377, 1399, 1715
PaeI	GCATGC	1 cut(s)	706
PaeR7I	CTCGAG	1 cut(s)	1155
PagI	TCATGA	1 cut(s)	1069
PaqCI	CACCTGC	1 cut(s)	796
PciI	ACATGT	1 cut(s)	1711
PciSI	GCTCTTC	1 cut(s)	176
PcsI	WCGNNNNNNNCGW	2 cut(s)	147, 1410
PctI	GAATGC	2 cut(s)	988, 1316
PdmI	GAANNNNTTC	2 cut(s)	81, 1231
PfeI	GAWTC	4 cut(s)	124, 132, 1227, 1352
PleI	GAGTC	2 cut(s)	686, 860
PmaCI	CACGTG	1 cut(s)	1405
PmlI	CACGTG	1 cut(s)	1405
PpsI	GAGTC	2 cut(s)	686, 860
Ppu21I	YACGTR	1 cut(s)	1405
PscI	ACATGT	1 cut(s)	1711
PshAI	GACNNNNGTC	1 cut(s)	1398
Psp124BI	GAGCTC	2 cut(s)	188, 262
Psp1406I	AACGTT	1 cut(s)	1413
Psp6I	CCWGG	2 cut(s)	253, 1848
PspCI	CACGTG	1 cut(s)	1405
PspGI	CCWGG	2 cut(s)	253, 1848
PspN4I	GGNNCC	5 cut(s)	445, 907, 1186, 1283, 1719
PspPI	GGNCC	3 cut(s)	147, 1185, 1717
PspXI	VCTCGAGB	1 cut(s)	1155
PstI	CTGCAG	3 cut(s)	400, 546, 1295
PsuI	RGATCY	1 cut(s)	1561
PvuII	CAGCTG	3 cut(s)	245, 644, 1296
RruI	TCGCGA	2 cut(s)	777, 828
RsaI	GTAC	3 cut(s)	496, 1143, 1877
RsaNI	GTAC	3 cut(s)	495, 1142, 1876
RseI	CAYNNNNRTG	2 cut(s)	1116, 1331
SacI	GAGCTC	2 cut(s)	188, 262
SapI	GCTCTTC	1 cut(s)	176
SaqAI	TTAA	5 cut(s)	500, 561, 753, 813, 1274
Sau3AI	GATC	7 cut(s)	275, 382, 613, 637, 1072, 1561, 1681
Sau96I	GGNCC	3 cut(s)	147, 1185, 1717
ScaI	AGTACT	1 cut(s)	496
SchI	GAGTC	2 cut(s)	686, 861
ScrFI	CCNGG	3 cut(s)	255, 441, 1850
SduI	GDGCHC	6 cut(s)	188, 262, 446, 528, 1284, 1486
SfaNI	GCATC	7 cut(s)	313, 1327, 1397, 1470, 1727, 1749, 1798
SfcI	CTRYAG	4 cut(s)	396, 542, 672, 1291
Sfr274I	CTCGAG	1 cut(s)	1155
SinI	GGWCC	2 cut(s)	1185, 1717
SlaI	CTCGAG	1 cut(s)	1155
SmiMI	CAYNNNNRTG	2 cut(s)	1116, 1331
SmlI	CTYRAG	2 cut(s)	1155, 1673
SmoI	CTYRAG	2 cut(s)	1155, 1673
SphI	GCATGC	1 cut(s)	706
SsiI	CCGC	7 cut(s)	112, 201, 359, 565, 1322, 1631, 1902
SspI	AATATT	1 cut(s)	795
SspMI	CTAG	3 cut(s)	339, 512, 1824
SstI	GAGCTC	2 cut(s)	188, 262
StyD4I	CCNGG	3 cut(s)	253, 439, 1848
TaaI	ACNGT	9 cut(s)	81, 290, 334, 506, 523, 676, 959, 1730, 1766
TaiI	ACGT	2 cut(s)	1407, 1416
TaqI	TCGA	8 cut(s)	27, 75, 135, 188, 274, 1156, 1225, 1366
TatI	WGTACW	2 cut(s)	494, 1875
TauI	GCSGC	4 cut(s)	115, 203, 361, 568
TfiI	GAWTC	4 cut(s)	124, 132, 1227, 1352
Tru1I	TTAA	5 cut(s)	500, 561, 753, 813, 1274
Tru9I	TTAA	5 cut(s)	500, 561, 753, 813, 1274
TscAI	CASTG	3 cut(s)	215, 1735, 1875
TseFI	GTSAC	4 cut(s)	737, 953, 1400, 1668
TseI	GCWGC	9 cut(s)	242, 398, 544, 568, 578, 644, 1293, 1582, 1811
Tsp45I	GTSAC	4 cut(s)	737, 953, 1400, 1668
TspGWI	ACGGA	3 cut(s)	21, 254, 1911
TspRI	CASTG	3 cut(s)	215, 1735, 1875
VpaK11BI	GGWCC	2 cut(s)	1185, 1717
XagI	CCTNNNNNAGG	1 cut(s)	93
XapI	RAATTY	4 cut(s)	458, 467, 1513, 1524
XceI	RCATGY	7 cut(s)	706, 1121, 1213, 1373, 1377, 1399, 1715
XcmI	CCANNNNNNNNNTGG	1 cut(s)	46
XhoI	CTCGAG	1 cut(s)	1155
XmiI	GTMKAC	1 cut(s)	671
XmnI	GAANNNNTTC	2 cut(s)	81, 1231
XspI	CTAG	3 cut(s)	339, 512, 1824
ZrmI	AGTACT	1 cut(s)	496
Zsp2I	ATGCAT	2 cut(s)	1375, 1742

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.