Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0010295

Physical Location & Seq

Genomic Coordinates Forward (+)

29907955 .. 29912142

Gene Structure 4188 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG277600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1968 bp

ATGGACGGAAAACTGGAAATCTCTCTGGACAAGCTCCCAATCAAGCGCCTAGACGTCATCGAAGAGAACGGCCTCGAACGATTTCCACCCGATGTAGGTTACGAGGAGAAGCGGGAGTCGCTGATTCGGAGAATCGATTTCGCTTGGGCGGTGGAGAAGGACGACAAGAACAACAAGCAGAAGAAGCGCTCCAGAGAGACCTCCGCCGCGCAATGGGAGTTTCAGAGCATGGTCGACAATCTAAAGTTGGCTCACCAAGAGCTCTCTGTCATTGTCGATCTCATCAATACTGTGGAAGCTAATGATGCAGTTACAGTAGCTAGCATGACCCGGCCGAGGCCATTTCCTAATGAAGCTCTGTCTGATCTTGCTGTGTCTGCCGCCACCAAGCTACAATGCTTCCGACATCTTGGAAAATACTTTAAGCAGTCTGCCAATGCTTTGGAGCAACAGATGGCCAGGGAAGCAAGATTTTATGGTGCACTAATCAGATTGCAGCAGAACTGGAAAGTTAAGCGGCAGCGTGCAGTAGCTTTAGCTTCTGGAAATGAAGGCTTCACCATAGACCTGTTTGACAATTCATTATATGATCCTGCTGGAATCTTTAGGCCATCCCCTCTGTCTACAGTTTGTGTTGACCATGACTCAGCTGGCATGCTGGCGATAAATTTACCTCCAAAGTCATATCGCTCTCTTCAGTTTGGTTTTCTCAGTGCTTATACAGATGATATGCCAATTGATTCCAGTAAAACCAAAACTCAATCCGCATGTGATGCTGACAAAGAATCTGTAAGTGATGACGAGTGTGTTAAAGAAACGCATTCACTTCTTCGTGAAGTCCATCAAGCGATCTTCAGTGAGCAGGTATTTGACTTGGTAAATCGTGAAGCATGTAACCAATCATTAGGAGTCACTGTTACTGGAATACAGGAAAACTATATACAATTGAATATAGGTCAAGAAACCTCTGTGTTCATATCCCTTGTCCCAACTAGTCAAGGTGAGGAAACAGTTGATAGTCTAAGCACCCAGAATTTAGAGAATGCAATTCTACCTTTGGATACACTGGATGGACTGAATTTACCAGAGGATAAACCAGATACCCCCAAGAAAAAGTCAGTAATTCCCAACCAAATTAGTTGTGAAATATATCTGCTACAGATATTTCATCAGCATGTATTTCTCAAAGCTAAGGATAGACCCACTTCCGGAGGTGGAGTATCTGGCCAACCGGCAAAAGAAGGGTCTGGTCTTCTTGGTCACTTCTGCATGTCTTTGGCTCATCGGATATTTTCAAACAAAGTTCTAATGGAGCTGGAAAATGTGGTTAGAGGTGTACCATATATGCAGTTGACATCTCATCCCACTTGGCATTCACGGACATCATCATGGACACTCTTTGTGAAGATACCTCAATCTATTCTACATGCATGCCAAACCCGAGCATCAGACTCACATCACAGGAAGCATGTTACCAAGTCTCAGTTTCATACTAAGGTGGTTGTAACTGATGATTGCATCAATGTCGAAGGGGAAGGTGCTCCTAATGTTGTAGGCCTGTTCAAAGGGACTTCCCAGGAAATCTGTTCAATGAATAGATATGACTGTGATTTGGCCGATCTTTCTGTGATAATTTTACAGCAGGTTGCAAGCCAAGTTATACGCTGGCTCCACGAAGAAGCTCTAATGGTTGGAATAAAAGCAAACTGGGACTTCCTATGTTTGTCTTTTGAGCTGGAGCAGGGTGAAACGCTAAGCTTGGTGGCACATGTGGATCCAGAAGATACTGAAGGATGCATCTCGTGGTGGTTGGTCATGGAGGATGGTTTTGTAGAAGAACGAAAACTCAGTTTGGACATCTCGGATGGTGCATCTGAGTCTAGGAAGTTCTTAGGCCATCTATCCCTGGAAGTGTTATATTCGACAATGATGGACTTGGTCGGCTTGTGCAATGGAGGTGGTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016070 GO:0016441 GO:0016458 GO:0016592 GO:0019222 GO:0031047 GO:0031050 GO:0031974 GO:0031981 GO:0032991 GO:0034641 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0046483 GO:0048519 GO:0050789 GO:0050896 GO:0051716 GO:0060255 GO:0065007 GO:0070013 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0090304 GO:1901360 GO:1901698 GO:1901699

KEGG Pathways

Metabolic & Signaling

ko04919 map04919

Pfam Domains

Protein Families

Med17

Protein Analysis

655

Amino Acids

73.14

Weight (kDa)

5.25

Isoelectric Point (pI)

41.35

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010295)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20170
fragaria_vesca	FvH4_5g23630 FvH4_5g23630 FvH4_5g23630 FvH4_5g23630
malus_domestica	MD02G1034800.v1.1 MD15G1175600.v1.1
prunus_persica	Prupe.7G240500_v2.0.a1
pyrus_communis	pycom02g02840 pycom15g15710
rosa_chinensis	RchiOBHm_Chr7g0212931
rosa_laevigata	RLG00000002843
rosa_multiflora	Rmu_co8301145.1_g000001 Rmu_sc0001684.1_g000012
rosa_roxburghii	Rroxscaffold_3G00246410
rosa_rugosa	Rorug07G0138300
rosa_samantha	Rh7AG269500 Rh7BG265000 Rh7CG288700 Rh7DG277600
rosa_wichuraiana	Rw7G023410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	57
Acc36I	ACCTGC	2 cut(s)	853, 1633
AccI	GTMKAC	2 cut(s)	234, 623
AccII	CGCG	1 cut(s)	209
AccIII	TCCGGA	1 cut(s)	1208
AciI	CCGC	7 cut(s)	112, 149, 204, 207, 381, 517, 765
AclWI	GGATC	3 cut(s)	584, 1769, 1782
AcoI	YGGCCR	4 cut(s)	332, 456, 1225, 1614
AcsI	RAATTY	3 cut(s)	667, 1033, 1078
AcuI	CTGAAG	3 cut(s)	680, 838, 1809
AcyI	GRCGYC	1 cut(s)	54
AfaI	GTAC	1 cut(s)	1338
AfeI	AGCGCT	1 cut(s)	188
AfiI	CCNNNNNNNGG	4 cut(s)	95, 213, 336, 1208
AflIII	ACRYGT	1 cut(s)	1768
AgsI	TTSAA	4 cut(s)	949, 1296, 1564, 1590
AhlI	ACTAGT	1 cut(s)	992
AjnI	CCWGG	3 cut(s)	458, 1575, 1905
Alw21I	GWGCWC	3 cut(s)	264, 484, 1543
Alw26I	GTCTC	2 cut(s)	191, 1485
Alw44I	GTGCAC	1 cut(s)	480
AlwI	GGATC	3 cut(s)	584, 1769, 1782
Ama87I	CYCGRG	1 cut(s)	1440
Aor13HI	TCCGGA	1 cut(s)	1208
Aor51HI	AGCGCT	1 cut(s)	188
AoxI	GGCC	9 cut(s)	70, 332, 338, 456, 608, 1225, 1555, 1614, 1894
ApaLI	GTGCAC	1 cut(s)	480
ApeKI	GCWGC	2 cut(s)	496, 520
ApoI	RAATTY	3 cut(s)	667, 1033, 1078
ArsI	GACNNNNNNTTYG	2 cut(s)	1229, 1261
Asp700I	GAANNNNTTC	1 cut(s)	81
AspLEI	GCGC	3 cut(s)	48, 189, 211
AsuC2I	CCSGG	1 cut(s)	331
AsuHPI	GGTGA	4 cut(s)	245, 550, 1013, 1757
AsuNHI	GCTAGC	1 cut(s)	320
AvaI	CYCGRG	1 cut(s)	1440
BaeGI	GKGCMC	1 cut(s)	484
BalI	TGGCCA	2 cut(s)	458, 1227
BamHI	GGATCC	1 cut(s)	1774
BanII	GRGCYC	1 cut(s)	264
BauI	CACGAG	1 cut(s)	1801
BbsI	GAAGAC	1 cut(s)	1244
Bbv12I	GWGCWC	3 cut(s)	264, 484, 1543
BbvI	GCAGC	2 cut(s)	508, 532
BccI	CCATC	8 cut(s)	448, 619, 849, 1064, 1817, 1859, 1905, 1924
BceAI	ACGGC	1 cut(s)	85
BcgI	CGANNNNNNTGC	2 cut(s)	1507, 1541
BciT130I	CCWGG	3 cut(s)	460, 1577, 1907
BciVI	GTATCC	1 cut(s)	1054
BcnI	CCSGG	1 cut(s)	331
BcoDI	GTCTC	2 cut(s)	191, 1485
BcuI	ACTAGT	1 cut(s)	992
BfaI	CTAG	4 cut(s)	50, 321, 993, 1881
BfmI	CTRYAG	2 cut(s)	624, 1157
BfoI	RGCGCY	2 cut(s)	49, 190
BfuAI	ACCTGC	2 cut(s)	853, 1633
BfuI	GTATCC	1 cut(s)	1054
BisI	GCNGC	5 cut(s)	207, 381, 497, 518, 521
BlpI	GCTNAGC	1 cut(s)	1754
BlsI	GCNGC	5 cut(s)	208, 382, 498, 519, 522
Bme1390I	CCNGG	4 cut(s)	331, 460, 1577, 1907
BmeT110I	CYCGRG	1 cut(s)	1440
BmiI	GGNNCC	2 cut(s)	1670, 1776
BmrFI	CCNGG	4 cut(s)	331, 460, 1577, 1907
BmrI	ACTGGG	1 cut(s)	1717
BmsI	GCATC	7 cut(s)	295, 763, 1454, 1527, 1784, 1806, 1880
BmtI	GCTAGC	1 cut(s)	324
BmuI	ACTGGG	1 cut(s)	1717
BpiI	GAAGAC	1 cut(s)	1244
BpmI	CTGGAG	2 cut(s)	175, 1757
Bpu10I	CCTNAGC	1 cut(s)	1191
Bpu1102I	GCTNAGC	1 cut(s)	1754
BpuMI	CCSGG	1 cut(s)	331
Bsa29I	ATCGAT	1 cut(s)	135
BsaHI	GRCGYC	1 cut(s)	54
BsaI	GGTCTC	1 cut(s)	191
BsaJI	CCNNGG	4 cut(s)	335, 459, 1575, 1905
BsaWI	WCCGGW	1 cut(s)	1208
BsaXI	ACNNNNNCTCC	6 cut(s)	900, 930, 1203, 1233, 1653, 1683
Bsc4I	CCNNNNNNNGG	4 cut(s)	95, 213, 336, 1208
Bse118I	RCCGGY	1 cut(s)	1231
Bse1I	ACTGG	6 cut(s)	18, 509, 744, 925, 1071, 1712
Bse3DI	GCAATG	2 cut(s)	218, 1957
BseAI	TCCGGA	1 cut(s)	1208
BseBI	CCWGG	3 cut(s)	460, 1577, 1907
BseCI	ATCGAT	1 cut(s)	135
BseDI	CCNNGG	4 cut(s)	335, 459, 1575, 1905
BseGI	GGATG	6 cut(s)	611, 1075, 1360, 1799, 1828, 1870
BseLI	CCNNNNNNNGG	4 cut(s)	95, 213, 336, 1208
BseMI	GCAATG	2 cut(s)	218, 1957
BseMII	CTCAG	5 cut(s)	660, 724, 1496, 1861, 1866
BseNI	ACTGG	6 cut(s)	18, 509, 744, 925, 1071, 1712
BseRI	GAGGAG	1 cut(s)	119
BseSI	GKGCMC	1 cut(s)	484
BseX3I	CGGCCG	1 cut(s)	332
BseXI	GCAGC	2 cut(s)	508, 532
BsgI	GTGCAG	1 cut(s)	546
Bsh1236I	CGCG	1 cut(s)	209
Bsh1285I	CGRYCG	1 cut(s)	335
BshFI	GGCC	9 cut(s)	72, 334, 340, 458, 610, 1227, 1557, 1616, 1896
BshVI	ATCGAT	1 cut(s)	135
BsiEI	CGRYCG	1 cut(s)	335
BsiHKAI	GWGCWC	3 cut(s)	264, 484, 1543
BsiHKCI	CYCGRG	1 cut(s)	1440
BsiSI	CCGG	3 cut(s)	331, 1209, 1232
BslFI	GGGAC	3 cut(s)	971, 1582, 1724
BslI	CCNNNNNNNGG	4 cut(s)	95, 213, 336, 1208
BsmAI	GTCTC	2 cut(s)	191, 1485
BsmFI	GGGAC	3 cut(s)	971, 1582, 1724
BsmI	GAATGC	3 cut(s)	820, 1048, 1372
BsnI	GGCC	9 cut(s)	72, 334, 340, 458, 610, 1227, 1557, 1616, 1896
Bso31I	GGTCTC	1 cut(s)	191
BsoBI	CYCGRG	1 cut(s)	1440
Bsp1286I	GDGCHC	3 cut(s)	264, 484, 1543
Bsp13I	TCCGGA	1 cut(s)	1208
Bsp143I	GATC	6 cut(s)	277, 364, 589, 849, 1618, 1774
Bsp1720I	GCTNAGC	1 cut(s)	1754
BspACI	CCGC	7 cut(s)	112, 149, 204, 207, 381, 517, 765
BspANI	GGCC	9 cut(s)	72, 334, 340, 458, 610, 1227, 1557, 1616, 1896
BspCNI	CTCAG	5 cut(s)	659, 723, 1495, 1860, 1867
BspDI	ATCGAT	1 cut(s)	135
BspEI	TCCGGA	1 cut(s)	1208
BspFNI	CGCG	1 cut(s)	209
BspLI	GGNNCC	2 cut(s)	1670, 1776
BspMI	ACCTGC	2 cut(s)	853, 1633
BspOI	GCTAGC	1 cut(s)	324
BspPI	GGATC	3 cut(s)	584, 1769, 1782
BspTNI	GGTCTC	1 cut(s)	191
BsrDI	GCAATG	2 cut(s)	218, 1957
BsrFI	RCCGGY	1 cut(s)	1231
BsrI	ACTGG	6 cut(s)	18, 509, 744, 925, 1071, 1712
BssAI	RCCGGY	1 cut(s)	1231
BssECI	CCNNGG	4 cut(s)	335, 459, 1575, 1905
BssMI	GATC	6 cut(s)	277, 364, 589, 849, 1618, 1774
BssNI	GRCGYC	1 cut(s)	54
BssSI	CACGAG	1 cut(s)	1801
Bst2BI	CACGAG	1 cut(s)	1801
Bst2UI	CCWGG	3 cut(s)	460, 1577, 1907
Bst4CI	ACNGT	6 cut(s)	292, 316, 628, 916, 1012, 1607
Bst6I	CTCTTC	2 cut(s)	57, 699
BstACI	GRCGYC	1 cut(s)	54
BstAPI	GCANNNNNTGC	1 cut(s)	773
BstC8I	GCNNGC	8 cut(s)	322, 525, 652, 656, 660, 1432, 1651, 1667
BstF5I	GGATG	6 cut(s)	611, 1075, 1360, 1799, 1828, 1870
BstFNI	CGCG	1 cut(s)	209
BstH2I	RGCGCY	2 cut(s)	49, 190
BstHHI	GCGC	3 cut(s)	48, 189, 211
BstKTI	GATC	6 cut(s)	280, 367, 592, 852, 1621, 1777
BstMAI	GTCTC	2 cut(s)	191, 1485
BstMBI	GATC	6 cut(s)	277, 364, 589, 849, 1618, 1774
BstMCI	CGRYCG	1 cut(s)	335
BstMWI	GCNNNNNNNGC	6 cut(s)	118, 184, 305, 377, 464, 773
BstNI	CCWGG	3 cut(s)	460, 1577, 1907
BstNSI	RCATGY	9 cut(s)	658, 771, 894, 1178, 1273, 1430, 1434, 1472, 1772
BstSCI	CCNGG	4 cut(s)	329, 458, 1575, 1905
BstSFI	CTRYAG	2 cut(s)	624, 1157
BstSLI	GKGCMC	1 cut(s)	484
BstUI	CGCG	1 cut(s)	209
BstV1I	GCAGC	2 cut(s)	508, 532
BstV2I	GAAGAC	1 cut(s)	1244
BstX2I	RGATCY	1 cut(s)	1774
BstXI	CCANNNNNNTGG	1 cut(s)	442
BstYI	RGATCY	1 cut(s)	1774
BstZI	CGGCCG	1 cut(s)	332
Bsu15I	ATCGAT	1 cut(s)	135
BsuI	GTATCC	1 cut(s)	1054
BsuRI	GGCC	9 cut(s)	72, 334, 340, 458, 610, 1227, 1557, 1616, 1896
BsuTUI	ATCGAT	1 cut(s)	135
BtsCI	GGATG	6 cut(s)	611, 1075, 1360, 1799, 1828, 1870
BtsIMutI	CAGTG	4 cut(s)	718, 862, 912, 1064
BveI	ACCTGC	2 cut(s)	853, 1633
Cac8I	GCNNGC	8 cut(s)	322, 525, 652, 656, 660, 1432, 1651, 1667
CfoI	GCGC	3 cut(s)	48, 189, 211
Cfr10I	RCCGGY	1 cut(s)	1231
ClaI	ATCGAT	1 cut(s)	135
Csp6I	GTAC	1 cut(s)	1337
CspCI	CAANNNNNGTGG	1 cut(s)	1939
CviQI	GTAC	1 cut(s)	1337
DpnI	GATC	6 cut(s)	279, 366, 591, 851, 1620, 1776
DpnII	GATC	6 cut(s)	277, 364, 589, 849, 1618, 1774
EaeI	YGGCCR	4 cut(s)	332, 456, 1225, 1614
EagI	CGGCCG	1 cut(s)	332
Eam1104I	CTCTTC	2 cut(s)	57, 699
EarI	CTCTTC	2 cut(s)	57, 699
EciI	GGCGGA	1 cut(s)	193
Ecl136II	GAGCTC	1 cut(s)	262
EclXI	CGGCCG	1 cut(s)	332
Eco147I	AGGCCT	1 cut(s)	1557
Eco24I	GRGCYC	1 cut(s)	264
Eco31I	GGTCTC	1 cut(s)	191
Eco47III	AGCGCT	1 cut(s)	188
Eco52I	CGGCCG	1 cut(s)	332
Eco53kI	GAGCTC	1 cut(s)	262
Eco57I	CTGAAG	3 cut(s)	680, 838, 1809
Eco88I	CYCGRG	1 cut(s)	1440
EcoICRI	GAGCTC	1 cut(s)	262
EcoRII	CCWGG	3 cut(s)	458, 1575, 1905
EcoT22I	ATGCAT	2 cut(s)	1432, 1799
EcoT38I	GRGCYC	1 cut(s)	264
FaqI	GGGAC	3 cut(s)	971, 1582, 1724
FauI	CCCGC	1 cut(s)	105
FblI	GTMKAC	2 cut(s)	234, 623
Fnu4HI	GCNGC	5 cut(s)	207, 381, 497, 518, 521
FokI	GGATG	6 cut(s)	598, 1082, 1347, 1806, 1835, 1877
FriOI	GRGCYC	1 cut(s)	264
Fsp4HI	GCNGC	5 cut(s)	207, 381, 497, 518, 521
FspBI	CTAG	4 cut(s)	50, 321, 993, 1881
GlaI	GCGC	3 cut(s)	47, 188, 210
GluI	GCNGC	5 cut(s)	207, 381, 497, 518, 521
GsuI	CTGGAG	2 cut(s)	175, 1757
HaeII	RGCGCY	2 cut(s)	49, 190
HaeIII	GGCC	9 cut(s)	72, 334, 340, 458, 610, 1227, 1557, 1616, 1896
HapII	CCGG	3 cut(s)	331, 1209, 1232
HhaI	GCGC	3 cut(s)	48, 189, 211
Hin1I	GRCGYC	1 cut(s)	54
Hin6I	GCGC	3 cut(s)	46, 187, 209
HinP1I	GCGC	3 cut(s)	46, 187, 209
HincII	GTYRAC	3 cut(s)	235, 637, 1353
HindII	GTYRAC	3 cut(s)	235, 637, 1353
HindIII	AAGCTT	1 cut(s)	1756
HpaII	CCGG	3 cut(s)	331, 1209, 1232
HphI	GGTGA	4 cut(s)	245, 550, 1013, 1757
Hpy166II	GTNNAC	6 cut(s)	235, 482, 624, 637, 1337, 1353
Hpy188I	TCNGA	9 cut(s)	129, 225, 364, 404, 491, 1287, 1450, 1864, 1876
Hpy188III	TCNNGA	8 cut(s)	26, 192, 543, 833, 884, 959, 1209, 1778
Hpy8I	GTNNAC	6 cut(s)	235, 482, 624, 637, 1337, 1353
HpyAV	CCTTC	6 cut(s)	151, 545, 1235, 1523, 1529, 1784
HpyCH4III	ACNGT	6 cut(s)	292, 316, 628, 916, 1012, 1607
HpyCH4IV	ACGT	1 cut(s)	54
HpyF10VI	GCNNNNNNNGC	6 cut(s)	118, 184, 305, 377, 464, 773
HpySE526I	ACGT	1 cut(s)	54
Hsp92I	GRCGYC	1 cut(s)	54
HspAI	GCGC	3 cut(s)	46, 187, 209
Kpn2I	TCCGGA	1 cut(s)	1208
Kzo9I	GATC	6 cut(s)	277, 364, 589, 849, 1618, 1774
LmnI	GCTCC	7 cut(s)	39, 194, 445, 1312, 1546, 1674, 1738
Lsp1109I	GCAGC	2 cut(s)	508, 532
LweI	GCATC	7 cut(s)	295, 763, 1454, 1527, 1784, 1806, 1880
MaeI	CTAG	4 cut(s)	50, 321, 993, 1881
MaeII	ACGT	1 cut(s)	54
MaeIII	GTNAC	9 cut(s)	98, 310, 893, 910, 916, 1259, 1471, 1504, 1961
MalI	GATC	6 cut(s)	279, 366, 591, 851, 1620, 1776
MboI	GATC	6 cut(s)	277, 364, 589, 849, 1618, 1774
MfeI	CAATTG	2 cut(s)	735, 944
MflI	RGATCY	1 cut(s)	1774
MhlI	GDGCHC	3 cut(s)	264, 484, 1543
MlsI	TGGCCA	2 cut(s)	458, 1227
MluNI	TGGCCA	2 cut(s)	458, 1227
MlyI	GAGTC	5 cut(s)	125, 638, 918, 1445, 1886
MmeI	TCCRAC	2 cut(s)	427, 1672
Mox20I	TGGCCA	2 cut(s)	458, 1227
Mph1103I	ATGCAT	2 cut(s)	1432, 1799
MroI	TCCGGA	1 cut(s)	1208
MroXI	GAANNNNTTC	1 cut(s)	81
MscI	TGGCCA	2 cut(s)	458, 1227
MseI	TTAA	3 cut(s)	423, 513, 810
MslI	CAYNNNNRTG	2 cut(s)	1173, 1387
Msp20I	TGGCCA	2 cut(s)	458, 1227
MspA1I	CMGCKG	1 cut(s)	650
MspI	CCGG	3 cut(s)	331, 1209, 1232
MspR9I	CCNGG	4 cut(s)	331, 460, 1577, 1907
MunI	CAATTG	2 cut(s)	735, 944
Mva1269I	GAATGC	3 cut(s)	820, 1048, 1372
MvaI	CCWGG	3 cut(s)	460, 1577, 1907
MvnI	CGCG	1 cut(s)	209
MwoI	GCNNNNNNNGC	6 cut(s)	118, 184, 305, 377, 464, 773
NciI	CCSGG	1 cut(s)	331
NdeII	GATC	6 cut(s)	277, 364, 589, 849, 1618, 1774
NheI	GCTAGC	1 cut(s)	320
NlaIV	GGNNCC	2 cut(s)	1670, 1776
NmeAIII	GCCGAG	1 cut(s)	360
NmuCI	GTSAC	2 cut(s)	910, 1259
NsiI	ATGCAT	2 cut(s)	1432, 1799
NspI	RCATGY	9 cut(s)	658, 771, 894, 1178, 1273, 1430, 1434, 1472, 1772
PaeI	GCATGC	2 cut(s)	658, 1434
PceI	AGGCCT	1 cut(s)	1557
PciI	ACATGT	1 cut(s)	1768
PctI	GAATGC	3 cut(s)	820, 1048, 1372
PdmI	GAANNNNTTC	1 cut(s)	81
PfeI	GAWTC	5 cut(s)	124, 132, 600, 740, 785
PflFI	GACNNNGTC	1 cut(s)	1937
PkrI	GCNGC	5 cut(s)	208, 382, 498, 519, 522
PleI	GAGTC	5 cut(s)	124, 638, 917, 1445, 1885
PpsI	GAGTC	5 cut(s)	124, 638, 917, 1445, 1885
PscI	ACATGT	1 cut(s)	1768
Psp124BI	GAGCTC	1 cut(s)	264
Psp6I	CCWGG	3 cut(s)	458, 1575, 1905
PspGI	CCWGG	3 cut(s)	458, 1575, 1905
PspN4I	GGNNCC	2 cut(s)	1670, 1776
PsuI	RGATCY	1 cut(s)	1774
PsyI	GACNNNGTC	1 cut(s)	1937
PvuII	CAGCTG	1 cut(s)	650
RsaI	GTAC	1 cut(s)	1338
RsaNI	GTAC	1 cut(s)	1337
RseI	CAYNNNNRTG	2 cut(s)	1173, 1387
SacI	GAGCTC	1 cut(s)	264
SalI	GTCGAC	1 cut(s)	233
SaqAI	TTAA	3 cut(s)	423, 513, 810
SatI	GCNGC	5 cut(s)	207, 381, 497, 518, 521
Sau3AI	GATC	6 cut(s)	277, 364, 589, 849, 1618, 1774
SchI	GAGTC	5 cut(s)	125, 638, 918, 1445, 1886
ScrFI	CCNGG	4 cut(s)	331, 460, 1577, 1907
SduI	GDGCHC	3 cut(s)	264, 484, 1543
SfaNI	GCATC	7 cut(s)	295, 763, 1454, 1527, 1784, 1806, 1880
SfcI	CTRYAG	2 cut(s)	624, 1157
SmiMI	CAYNNNNRTG	2 cut(s)	1173, 1387
SpeI	ACTAGT	1 cut(s)	992
SphI	GCATGC	2 cut(s)	658, 1434
SseBI	AGGCCT	1 cut(s)	1557
SsiI	CCGC	7 cut(s)	112, 149, 204, 207, 381, 517, 765
SspMI	CTAG	4 cut(s)	50, 321, 993, 1881
SstI	GAGCTC	1 cut(s)	264
StuI	AGGCCT	1 cut(s)	1557
StyD4I	CCNGG	4 cut(s)	329, 458, 1575, 1905
TaaI	ACNGT	6 cut(s)	292, 316, 628, 916, 1012, 1607
TaiI	ACGT	1 cut(s)	57
TaqI	TCGA	7 cut(s)	60, 75, 135, 234, 276, 1527, 1922
TauI	GCSGC	3 cut(s)	209, 383, 520
TfiI	GAWTC	5 cut(s)	124, 132, 600, 740, 785
Tru1I	TTAA	3 cut(s)	423, 513, 810
Tru9I	TTAA	3 cut(s)	423, 513, 810
TscAI	CASTG	4 cut(s)	718, 862, 919, 1071
TseFI	GTSAC	2 cut(s)	910, 1259
TseI	GCWGC	2 cut(s)	496, 520
Tsp45I	GTSAC	2 cut(s)	910, 1259
TspDTI	ATGAA	7 cut(s)	366, 564, 570, 964, 1157, 1478, 1607
TspGWI	ACGGA	2 cut(s)	21, 1393
TspRI	CASTG	4 cut(s)	718, 862, 919, 1071
Tth111I	GACNNNGTC	1 cut(s)	1937
VneI	GTGCAC	1 cut(s)	480
XapI	RAATTY	3 cut(s)	667, 1033, 1078
XceI	RCATGY	9 cut(s)	658, 771, 894, 1178, 1273, 1430, 1434, 1472, 1772
XcmI	CCANNNNNNNNNTGG	1 cut(s)	647
XmiI	GTMKAC	2 cut(s)	234, 623
XmnI	GAANNNNTTC	1 cut(s)	81
XspI	CTAG	4 cut(s)	50, 321, 993, 1881
ZraI	GACGTC	1 cut(s)	55
Zsp2I	ATGCAT	2 cut(s)	1432, 1799

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.