Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0016643

Physical Location & Seq

Genomic Coordinates Reverse (-)

2796503 .. 2797870

Gene Structure 1368 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1037000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1368 bp

ATGAAGGACAGAAAAGCTAACATAGTAGCTTCATCGTCGATCATCGCGCTCGTTGTTTTTATACTCGTTGCGCGAATCACTCTCAAACTTTCGCCAACTTTCTTCCTCATTTGCGGGGCAGGTGTTGCTGTGATCCTTGCTGTGTTTGTATGGCTAGCGATCAGACACCATTACAATTGCCGGAGAAAGCTCTTGGAATCAAAATACGCGTCAGAAGGAAAGGAACTTCGCATGGAGTATAGTTTTCTTCGAAAAGTTGCGGGGGTTCCCACGAAGTTTAGGTACAAGGAGATAGAGGATGCAACAGATCATTTCAAGGCATTGCTTGGGAAAGGGGCTTCAGCTTCTGTTTACAAAGGGATCCTAAGTGATGGGACTCAAGTGGCAGTGAAAAAGCTCGATGTAGAGGAGCGCGGCGAGAGGGAGTTCAGGTCAGAAGTTGCGGCGATTACAAGCCTGCAACATATCAATCTTGTGCGCCTTCTCGGGTATTGTTGTGTTCCTGCAGGACCACGATTCTTGGTTTATGATTTCATCCCAAATGGATCGTTGGATTCTTGGATTTTCTCTCAGAAGGGAAGGCCTAATCGACGTGGGGGGTGCTTGCCTTGGGACACAAGGTACAGGGTTGCAGTTGACGTTGCTAGAGCGCTTTCTTACCTGCATCGTGATTGCAGGTCAAGGATTTTGCACCTTGATGTGAAGCCAGAGAACATCCTTTTGGACGAGAATTACAGAGCAATTGTCGCGGATTTTGGCCTTTCGAAGTTAATGGGAAAAGACGAGAGTAGAATCATGATCACGATTCGGGGGACCAGGGGCTACTTGGCTCCTGAGTGGCTCTTGGAGCATGGAATTTCCGAAAAAACAGATGTGTACAGTTACGGAATGGTGCTTCTTGAGATGATCGGAGGGCGGAGAAACATTAGCTTCGTCGAAAAGGGCAATGCCACCAACAAGTCCCAGAGGAAAGGGGAGTATTTTCCCAAAATTGTGATCGAGAAAATGGGAGAAGGTAAGCTTATGGAAGTTGTTGATCAAAGGCTGATCGAAAGTGGACGCGTTGACGAAAGGGAGGTAAAAAAACTTGTTCATATAGCTCTATGGTGCGTACAAGAGAGGGCTAGGCTTAGGCCTACCATGTCACATGTGGTGGAGATGCTTGAGGGCCGGACGCCCGTGGAGGAGCCTCCCGACACCCAAATGGTGGTTGTTGATTTTTTGGCGATGGACGACGAGACGAATGACGGCAACAAGAGGGCAAAGATTGGTAGTGCAGCCTTGGCAACACAAGAAGTAGAGAGCAATGATGCATCCACATCAACATGTTCATATGGAAATGCAATGTCCATGATAGCCCCAAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase

Protein Analysis

456

Amino Acids

51.21

Weight (kDa)

9.07

Isoelectric Point (pI)

36.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	105 - 388	4.8e-37	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	106 - 386	1.2e-41	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016643)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20050
fragaria_vesca	FvH4_1g03860
malus_domestica	MD02G1037000.v1.1 MD15G1177100.v1.1
prunus_persica	Prupe.7G238400_v2.0.a1 Prupe.7G238400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0089251
rosa_laevigata	RLG00000016008
rosa_multiflora	Rmu_sc0008322.1_g000020
rosa_rugosa	Rorug01G0488600
rosa_samantha	Rh2AG044600 Rh2BG043300 Rh2DG043600
rosa_wichuraiana	Rw2G003860

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	110
Acc36I	ACCTGC	3 cut(s)	110, 666, 669
AccB7I	CCANNNNNTGG	1 cut(s)	1207
AccII	CGCG	6 cut(s)	47, 73, 209, 414, 749, 1062
AciI	CCGC	6 cut(s)	114, 260, 414, 443, 749, 916
AclWI	GGATC	4 cut(s)	127, 355, 368, 553
AcsI	RAATTY	1 cut(s)	855
AcuI	CTGAAG	1 cut(s)	324
AcyI	GRCGYC	1 cut(s)	1175
AfaI	GTAC	4 cut(s)	284, 623, 878, 1113
AfeI	AGCGCT	1 cut(s)	651
AfiI	CCNNNNNNNGG	1 cut(s)	1207
AflIII	ACRYGT	4 cut(s)	207, 1060, 1147, 1325
AgsI	TTSAA	1 cut(s)	316
AjiI	CACGTC	1 cut(s)	593
AjnI	CCWGG	1 cut(s)	815
AjuI	GAANNNNNNNTTGG	2 cut(s)	704, 736
AluBI	AGCT	8 cut(s)	17, 29, 190, 344, 397, 930, 1021, 1100
AluI	AGCT	8 cut(s)	17, 29, 190, 344, 397, 930, 1021, 1100
Alw26I	GTCTC	1 cut(s)	1232
AlwI	GGATC	4 cut(s)	127, 355, 368, 553
AlwNI	CAGNNNCTG	1 cut(s)	347
Ama87I	CYCGRG	1 cut(s)	485
Aor51HI	AGCGCT	1 cut(s)	651
AoxI	GGCC	4 cut(s)	581, 757, 1133, 1168
ApeKI	GCWGC	1 cut(s)	1277
ApoI	RAATTY	1 cut(s)	855
ArsI	GACNNNNNNTTYG	2 cut(s)	194, 226
AspLEI	GCGC	5 cut(s)	49, 73, 414, 480, 652
AspS9I	GGNCC	3 cut(s)	509, 813, 1168
AsuII	TTCGAA	2 cut(s)	250, 764
AsuNHI	GCTAGC	1 cut(s)	154
AvaI	CYCGRG	1 cut(s)	485
AvaII	GGWCC	2 cut(s)	509, 813
BamHI	GGATCC	1 cut(s)	360
BarI	GAAGNNNNNNTAC	2 cut(s)	266, 298
BbvI	GCAGC	1 cut(s)	1289
BccI	CCATC	2 cut(s)	365, 1222
BceAI	ACGGC	1 cut(s)	1264
BciT130I	CCWGG	1 cut(s)	817
BclI	TGATCA	2 cut(s)	798, 1036
BcoDI	GTCTC	1 cut(s)	1232
BfaI	CTAG	3 cut(s)	155, 645, 1125
BfmI	CTRYAG	1 cut(s)	504
BfoI	RGCGCY	1 cut(s)	653
BfuAI	ACCTGC	3 cut(s)	110, 666, 669
BisI	GCNGC	3 cut(s)	415, 444, 1278
BlsI	GCNGC	3 cut(s)	416, 445, 1279
Bme1390I	CCNGG	1 cut(s)	817
Bme18I	GGWCC	2 cut(s)	509, 813
BmeT110I	CYCGRG	1 cut(s)	485
BmgBI	CACGTC	1 cut(s)	593
BmgT120I	GGNCC	3 cut(s)	509, 813, 1168
BmiI	GGNNCC	5 cut(s)	267, 362, 814, 831, 1188
BmrFI	CCNGG	1 cut(s)	817
BmsI	GCATC	5 cut(s)	289, 673, 1149, 1300, 1322
BmtI	GCTAGC	1 cut(s)	158
Bpu10I	CCTNAGC	1 cut(s)	1130
Bpu14I	TTCGAA	2 cut(s)	250, 764
BpuEI	CTTGAG	3 cut(s)	363, 920, 1184
BsaHI	GRCGYC	1 cut(s)	1175
BsaJI	CCNNGG	4 cut(s)	608, 816, 1179, 1281
BsaXI	ACNNNNNCTCC	2 cut(s)	416, 446
Bsc4I	CCNNNNNNNGG	1 cut(s)	1207
Bse3DI	GCAATG	4 cut(s)	320, 952, 1312, 1350
BseBI	CCWGG	1 cut(s)	817
BseDI	CCNNGG	4 cut(s)	608, 816, 1179, 1281
BseGI	GGATG	4 cut(s)	304, 534, 714, 1313
BseLI	CCNNNNNNNGG	1 cut(s)	1207
BseMI	GCAATG	4 cut(s)	320, 952, 1312, 1350
BseMII	CTCAG	2 cut(s)	584, 825
BseRI	GAGGAG	2 cut(s)	422, 1199
BseXI	GCAGC	1 cut(s)	1289
BsgI	GTGCAG	1 cut(s)	1296
Bsh1236I	CGCG	6 cut(s)	47, 73, 209, 414, 749, 1062
BshFI	GGCC	4 cut(s)	583, 759, 1135, 1170
BsiHKCI	CYCGRG	1 cut(s)	485
BsiSI	CCGG	2 cut(s)	181, 1171
BslFI	GGGAC	4 cut(s)	388, 626, 826, 946
BslI	CCNNNNNNNGG	1 cut(s)	1207
BsmAI	GTCTC	1 cut(s)	1232
BsmBI	CGTCTC	1 cut(s)	1232
BsmFI	GGGAC	4 cut(s)	388, 626, 826, 946
BsnI	GGCC	4 cut(s)	583, 759, 1135, 1170
BsoBI	CYCGRG	1 cut(s)	485
Bsp119I	TTCGAA	2 cut(s)	250, 764
Bsp1407I	TGTACA	1 cut(s)	876
BspACI	CCGC	6 cut(s)	114, 260, 414, 443, 749, 916
BspANI	GGCC	4 cut(s)	583, 759, 1135, 1170
BspCNI	CTCAG	2 cut(s)	583, 826
BspFNI	CGCG	6 cut(s)	47, 73, 209, 414, 749, 1062
BspHI	TCATGA	1 cut(s)	795
BspLI	GGNNCC	5 cut(s)	267, 362, 814, 831, 1188
BspMAI	CTGCAG	1 cut(s)	508
BspMI	ACCTGC	3 cut(s)	110, 666, 669
BspOI	GCTAGC	1 cut(s)	158
BspPI	GGATC	4 cut(s)	127, 355, 368, 553
BspT104I	TTCGAA	2 cut(s)	250, 764
BsrDI	GCAATG	4 cut(s)	320, 952, 1312, 1350
BsrGI	TGTACA	1 cut(s)	876
BssECI	CCNNGG	4 cut(s)	608, 816, 1179, 1281
BssNI	GRCGYC	1 cut(s)	1175
BssT1I	CCWWGG	2 cut(s)	608, 1281
Bst2UI	CCWGG	1 cut(s)	817
Bst4CI	ACNGT	1 cut(s)	881
BstACI	GRCGYC	1 cut(s)	1175
BstAPI	GCANNNNNTGC	1 cut(s)	125
BstAUI	TGTACA	1 cut(s)	876
BstBI	TTCGAA	2 cut(s)	250, 764
BstC8I	GCNNGC	3 cut(s)	156, 458, 605
BstDEI	CTNAG	4 cut(s)	365, 570, 834, 1130
BstDSI	CCRYGG	1 cut(s)	1179
BstF5I	GGATG	4 cut(s)	304, 534, 714, 1313
BstFNI	CGCG	6 cut(s)	47, 73, 209, 414, 749, 1062
BstH2I	RGCGCY	1 cut(s)	653
BstHHI	GCGC	5 cut(s)	49, 73, 414, 480, 652
BstMAI	GTCTC	1 cut(s)	1232
BstMWI	GCNNNNNNNGC	4 cut(s)	125, 746, 847, 1283
BstNI	CCWGG	1 cut(s)	817
BstNSI	RCATGY	2 cut(s)	1151, 1329
BstSCI	CCNGG	1 cut(s)	815
BstSFI	CTRYAG	1 cut(s)	504
BstUI	CGCG	6 cut(s)	47, 73, 209, 414, 749, 1062
BstV1I	GCAGC	1 cut(s)	1289
BstX2I	RGATCY	1 cut(s)	360
BstYI	RGATCY	1 cut(s)	360
BsuRI	GGCC	4 cut(s)	583, 759, 1135, 1170
BtgI	CCRYGG	1 cut(s)	1179
BtgZI	GCGATG	2 cut(s)	28, 1241
BtrI	CACGTC	1 cut(s)	593
BtsCI	GGATG	4 cut(s)	304, 534, 714, 1313
BtsI	GCAGTG	1 cut(s)	393
BtsIMutI	CAGTG	1 cut(s)	393
BveI	ACCTGC	3 cut(s)	110, 666, 669
Cac8I	GCNNGC	3 cut(s)	156, 458, 605
CaiI	CAGNNNCTG	1 cut(s)	347
CciI	TCATGA	1 cut(s)	795
CfoI	GCGC	5 cut(s)	49, 73, 414, 480, 652
Cfr13I	GGNCC	3 cut(s)	509, 813, 1168
CseI	GACGC	3 cut(s)	198, 1068, 1183
Csp6I	GTAC	4 cut(s)	283, 622, 877, 1112
CspCI	CAANNNNNGTGG	2 cut(s)	501, 536
CviAII	CATG	7 cut(s)	232, 796, 851, 1141, 1148, 1326, 1351
CviQI	GTAC	4 cut(s)	283, 622, 877, 1112
DdeI	CTNAG	4 cut(s)	365, 570, 834, 1130
EciI	GGCGGA	1 cut(s)	931
Eco130I	CCWWGG	2 cut(s)	608, 1281
Eco147I	AGGCCT	2 cut(s)	583, 1135
Eco47I	GGWCC	2 cut(s)	509, 813
Eco47III	AGCGCT	1 cut(s)	651
Eco57I	CTGAAG	1 cut(s)	324
Eco88I	CYCGRG	1 cut(s)	485
EcoRII	CCWGG	1 cut(s)	815
EcoT14I	CCWWGG	2 cut(s)	608, 1281
EcoT22I	ATGCAT	1 cut(s)	1315
ErhI	CCWWGG	2 cut(s)	608, 1281
Esp3I	CGTCTC	1 cut(s)	1232
FaeI	CATG	7 cut(s)	235, 799, 854, 1144, 1151, 1329, 1354
FalI	AAGNNNNNCTT	2 cut(s)	1005, 1037
FaqI	GGGAC	4 cut(s)	388, 626, 826, 946
FatI	CATG	7 cut(s)	231, 795, 850, 1140, 1147, 1325, 1350
FauI	CCCGC	2 cut(s)	107, 253
FauNDI	CATATG	1 cut(s)	1333
FbaI	TGATCA	2 cut(s)	798, 1036
Fnu4HI	GCNGC	3 cut(s)	415, 444, 1278
FokI	GGATG	4 cut(s)	311, 521, 701, 1300
Fsp4HI	GCNGC	3 cut(s)	415, 444, 1278
FspBI	CTAG	3 cut(s)	155, 645, 1125
GlaI	GCGC	5 cut(s)	48, 72, 413, 479, 651
GluI	GCNGC	3 cut(s)	415, 444, 1278
HaeII	RGCGCY	1 cut(s)	653
HaeIII	GGCC	4 cut(s)	583, 759, 1135, 1170
HapII	CCGG	2 cut(s)	181, 1171
HgaI	GACGC	3 cut(s)	198, 1068, 1183
HhaI	GCGC	5 cut(s)	49, 73, 414, 480, 652
Hin1I	GRCGYC	1 cut(s)	1175
Hin1II	CATG	7 cut(s)	235, 799, 854, 1144, 1151, 1329, 1354
Hin6I	GCGC	5 cut(s)	47, 71, 412, 478, 650
HinP1I	GCGC	5 cut(s)	47, 71, 412, 478, 650
HincII	GTYRAC	2 cut(s)	637, 1066
HindII	GTYRAC	2 cut(s)	637, 1066
HindIII	AAGCTT	1 cut(s)	1019
HinfI	GANTC	7 cut(s)	75, 197, 376, 516, 554, 792, 805
HpaII	CCGG	2 cut(s)	181, 1171
Hpy166II	GTNNAC	5 cut(s)	352, 637, 877, 1058, 1066
Hpy188I	TCNGA	6 cut(s)	164, 214, 436, 573, 862, 911
Hpy188III	TCNNGA	7 cut(s)	668, 796, 802, 833, 899, 1000, 1193
Hpy8I	GTNNAC	5 cut(s)	352, 637, 877, 1058, 1066
Hpy99I	CGWCG	4 cut(s)	40, 594, 938, 1238
HpyAV	CCTTC	5 cut(s)	209, 491, 568, 573, 1007
HpyCH4III	ACNGT	1 cut(s)	881
HpyCH4IV	ACGT	2 cut(s)	592, 639
HpyF10VI	GCNNNNNNNGC	4 cut(s)	125, 746, 847, 1283
HpyF3I	CTNAG	4 cut(s)	365, 570, 834, 1130
HpySE526I	ACGT	2 cut(s)	592, 639
Hsp92I	GRCGYC	1 cut(s)	1175
Hsp92II	CATG	7 cut(s)	235, 799, 854, 1144, 1151, 1329, 1354
HspAI	GCGC	5 cut(s)	47, 71, 412, 478, 650
Ksp22I	TGATCA	2 cut(s)	798, 1036
LmnI	GCTCC	4 cut(s)	409, 835, 847, 1186
Lsp1109I	GCAGC	1 cut(s)	1289
LweI	GCATC	5 cut(s)	289, 673, 1149, 1300, 1322
MaeI	CTAG	3 cut(s)	155, 645, 1125
MaeII	ACGT	2 cut(s)	592, 639
MaeIII	GTNAC	2 cut(s)	881, 1143
MboII	GAAGA	2 cut(s)	94, 239
MfeI	CAATTG	2 cut(s)	175, 741
MflI	RGATCY	1 cut(s)	360
MluCI	AATT	5 cut(s)	175, 730, 741, 855, 990
MluI	ACGCGT	2 cut(s)	207, 1060
MlyI	GAGTC	1 cut(s)	370
MmeI	TCCRAC	1 cut(s)	531
Mph1103I	ATGCAT	1 cut(s)	1315
MseI	TTAA	1 cut(s)	770
MslI	CAYNNNNRTG	3 cut(s)	696, 1202, 1324
MspI	CCGG	2 cut(s)	181, 1171
MspR9I	CCNGG	1 cut(s)	817
MunI	CAATTG	2 cut(s)	175, 741
MvaI	CCWGG	1 cut(s)	817
MvnI	CGCG	6 cut(s)	47, 73, 209, 414, 749, 1062
MwoI	GCNNNNNNNGC	4 cut(s)	125, 746, 847, 1283
NdeI	CATATG	1 cut(s)	1333
NheI	GCTAGC	1 cut(s)	154
NlaIII	CATG	7 cut(s)	235, 799, 854, 1144, 1151, 1329, 1354
NlaIV	GGNNCC	5 cut(s)	267, 362, 814, 831, 1188
NmuCI	GTSAC	1 cut(s)	1143
NsiI	ATGCAT	1 cut(s)	1315
NspI	RCATGY	2 cut(s)	1151, 1329
NspV	TTCGAA	2 cut(s)	250, 764
PagI	TCATGA	1 cut(s)	795
PaqCI	CACCTGC	1 cut(s)	110
PceI	AGGCCT	2 cut(s)	583, 1135
PciI	ACATGT	2 cut(s)	1147, 1325
PfeI	GAWTC	6 cut(s)	75, 197, 516, 554, 792, 805
PflMI	CCANNNNNTGG	1 cut(s)	1207
PkrI	GCNGC	3 cut(s)	416, 445, 1279
PleI	GAGTC	1 cut(s)	370
PpsI	GAGTC	1 cut(s)	370
PscI	ACATGT	2 cut(s)	1147, 1325
Psp6I	CCWGG	1 cut(s)	815
PspGI	CCWGG	1 cut(s)	815
PspN4I	GGNNCC	5 cut(s)	267, 362, 814, 831, 1188
PspPI	GGNCC	3 cut(s)	509, 813, 1168
PstI	CTGCAG	1 cut(s)	508
PstNI	CAGNNNCTG	1 cut(s)	347
PsuI	RGATCY	1 cut(s)	360
RsaI	GTAC	4 cut(s)	284, 623, 878, 1113
RsaNI	GTAC	4 cut(s)	283, 622, 877, 1112
RseI	CAYNNNNRTG	3 cut(s)	696, 1202, 1324
SaqAI	TTAA	1 cut(s)	770
SatI	GCNGC	3 cut(s)	415, 444, 1278
Sau96I	GGNCC	3 cut(s)	509, 813, 1168
SbfI	CCTGCAGG	1 cut(s)	508
SchI	GAGTC	1 cut(s)	370
ScrFI	CCNGG	1 cut(s)	817
SdaI	CCTGCAGG	1 cut(s)	508
SfaNI	GCATC	5 cut(s)	289, 673, 1149, 1300, 1322
SfcI	CTRYAG	1 cut(s)	504
SfuI	TTCGAA	2 cut(s)	250, 764
SinI	GGWCC	2 cut(s)	509, 813
SmiMI	CAYNNNNRTG	3 cut(s)	696, 1202, 1324
SmlI	CTYRAG	3 cut(s)	378, 899, 1163
SmoI	CTYRAG	3 cut(s)	378, 899, 1163
Sse8387I	CCTGCAGG	1 cut(s)	508
Sse9I	AATT	5 cut(s)	175, 730, 741, 855, 990
SseBI	AGGCCT	2 cut(s)	583, 1135
SsiI	CCGC	6 cut(s)	114, 260, 414, 443, 749, 916
SspMI	CTAG	3 cut(s)	155, 645, 1125
StuI	AGGCCT	2 cut(s)	583, 1135
StyD4I	CCNGG	1 cut(s)	815
StyI	CCWWGG	2 cut(s)	608, 1281
TaaI	ACNGT	1 cut(s)	881
TaiI	ACGT	2 cut(s)	595, 642
TaqI	TCGA	8 cut(s)	38, 250, 399, 589, 764, 936, 999, 1050
TasI	AATT	5 cut(s)	175, 730, 741, 855, 990
TatI	WGTACW	1 cut(s)	876
TauI	GCSGC	2 cut(s)	417, 446
TfiI	GAWTC	6 cut(s)	75, 197, 516, 554, 792, 805
Tru1I	TTAA	1 cut(s)	770
Tru9I	TTAA	1 cut(s)	770
TscAI	CASTG	1 cut(s)	393
TseFI	GTSAC	1 cut(s)	1143
TseI	GCWGC	1 cut(s)	1277
Tsp45I	GTSAC	1 cut(s)	1143
TspDTI	ATGAA	5 cut(s)	17, 21, 523, 1082, 1320
TspGWI	ACGGA	1 cut(s)	900
TspRI	CASTG	1 cut(s)	393
Van91I	CCANNNNNTGG	1 cut(s)	1207
VpaK11BI	GGWCC	2 cut(s)	509, 813
XapI	RAATTY	1 cut(s)	855
XceI	RCATGY	2 cut(s)	1151, 1329
XcmI	CCANNNNNNNNNTGG	2 cut(s)	823, 1147
XspI	CTAG	3 cut(s)	155, 645, 1125
Zsp2I	ATGCAT	1 cut(s)	1315

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.