MD02G1076600.v1.1

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016071
Physical Location & Seq
Forward (+)
6059391 .. 6062833
3443 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1076600.v1.1.491

Sequence Viewer

Length: 810 bp
ATGGGAGTGAGCAACAGGATAACCGCAACTCTTAACTTCATAGCCCTCCTCTGCGCCATCCCAATAATGTCTTCCGGCATCTGGCTGGCCTCCAAGCCGGACAACGAGTGCATCCGCTCGTTCCGATGGCCGGTATTCATCCTCGGCGCCCTCATGCTCCTCGTCTGCCTCGCCGGCTTCATTGGCGCCTATTTCAACAAACAGGGTCTTTTGGCTCTCTACCTTTTCTGCATGGCGGCCCTCATAATCCTGCTCCTTGTCCTGCTCATCTTCGCCTTCACCGTCACGAGGCCGGACGGCAGCTACACCGTGCCCGGCAGGGCTTACAGGGAGTACCGGCTTGAAGGGTTTTCCAAGTGGCTGAGGGACTATGTTACTGACTCTTCGACTTGGCAGAAAATAAGGACTTGTTTGGCTGAGTCTGATACTTGCCCTAAGCTTACACAACAGTACATGTCAGCTGATCAGTTCCTCTTTGCTCACATCTCTCCCCTTCAGTCAGGATGTTGTGAACCTCCAACAATGTGTGGATACAACTATGTGAATCCAACACTGTGGATAAACCCAGTGAACCCAGGAGCTGACCCGGACTGCTTGATATGGAACAATGACCAGAACCTCTTGTGCTACAACTGCAATTCCTGCAGGGCTGGACTTTTGGGAAACCTGAGGAAAGAATGGAGGAGGGTCAATGTCATCCTCATTGTGGCTGTGGTGATCCTCATCTGCGTCTATCTCGTCGCCTGTAGCGCCTTCAAGAACGCCCAAACGGAGGAACTCTTCCGCCGGAACAAGCAGGGTAGGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

270

Amino Acids

30.3

Weight (kDa)

8.71

Isoelectric Point (pI)

46.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 7 - 250 7.4e-34 Tetraspanin family
DUF2207_C PF20990 48 - 124 8.6e-07 Predicted membrane protein (DUF2207) C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016071)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G19580
fragaria_vesca FvH4_1g07210 FvH4_1g07210
malus_domestica MD02G1076600.v1.1 MD15G1205300.v1.1
prunus_persica Prupe.7G211800_v2.0.a1
pyrus_communis pycom02g06090
rosa_laevigata RLG00000016369
rosa_multiflora Rmu_sc0006865.1_g000011
rosa_roxburghii Rroxscaffold_2G00148500
rosa_rugosa Rorug02G0032900
rosa_samantha Rh2AG078400 Rh2CG082000 Rh2DG077300
rosa_wichuraiana Rw2G006810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 146, 185
AccBSI CCGCTC 1 cut(s) 117
AciI CCGC 4 cut(s) 24, 115, 236, 784
AclWI GGATC 1 cut(s) 712
AcoI YGGCCR 1 cut(s) 128
AcuI CTGAAG 1 cut(s) 479
AcyI GRCGYC 2 cut(s) 147, 186
AfaI GTAC 2 cut(s) 335, 452
AfiI CCNNNNNNNGG 5 cut(s) 81, 130, 288, 706, 772
AflIII ACRYGT 1 cut(s) 453
AgsI TTSAA 3 cut(s) 196, 344, 757
AjnI CCWGG 1 cut(s) 574
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AluBI AGCT 4 cut(s) 303, 439, 461, 581
AluI AGCT 4 cut(s) 303, 439, 461, 581
AlwI GGATC 1 cut(s) 712
AlwNI CAGNNNCTG 1 cut(s) 581
AoxI GGCC 4 cut(s) 87, 128, 237, 290
ApeKI GCWGC 1 cut(s) 300
AspLEI GCGC 4 cut(s) 56, 149, 188, 752
AspS9I GGNCC 1 cut(s) 238
AsuC2I CCSGG 2 cut(s) 315, 587
AsuHPI GGTGA 2 cut(s) 271, 727
AxyI CCTNAGG 1 cut(s) 668
BaeGI GKGCMC 1 cut(s) 315
BanI GGYRCC 2 cut(s) 146, 185
BauI CACGAG 1 cut(s) 286
BbsI GAAGAC 1 cut(s) 63
BbvCI CCTCAGC 1 cut(s) 362
BbvI GCAGC 1 cut(s) 312
BccI CCATC 2 cut(s) 65, 120
BceAI ACGGC 1 cut(s) 313
BciT130I CCWGG 1 cut(s) 576
BciVI GTATCC 1 cut(s) 524
BclI TGATCA 1 cut(s) 463
BcnI CCSGG 2 cut(s) 315, 587
BfmI CTRYAG 2 cut(s) 643, 745
BfoI RGCGCY 3 cut(s) 150, 189, 753
BfuI GTATCC 1 cut(s) 524
BglI GCCNNNNNGGC 1 cut(s) 174
BisI GCNGC 2 cut(s) 237, 301
BlsI GCNGC 2 cut(s) 238, 302
Bme1390I CCNGG 3 cut(s) 315, 576, 587
BmgT120I GGNCC 1 cut(s) 238
BmiI GGNNCC 2 cut(s) 148, 187
BmrFI CCNGG 3 cut(s) 315, 576, 587
BmrI ACTGGG 1 cut(s) 560
BmsI GCATC 2 cut(s) 87, 120
BmuI ACTGGG 1 cut(s) 560
BpiI GAAGAC 1 cut(s) 63
Bpu10I CCTNAGC 2 cut(s) 362, 435
BpuMI CCSGG 2 cut(s) 315, 587
BsaBI GATNNNNATC 1 cut(s) 722
BsaHI GRCGYC 2 cut(s) 147, 186
BsaJI CCNNGG 2 cut(s) 142, 574
Bsc4I CCNNNNNNNGG 5 cut(s) 81, 130, 288, 706, 772
Bse118I RCCGGY 3 cut(s) 130, 173, 336
Bse1I ACTGG 1 cut(s) 566
Bse21I CCTNAGG 1 cut(s) 668
Bse8I GATNNNNATC 1 cut(s) 722
BseBI CCWGG 1 cut(s) 576
BseDI CCNNGG 2 cut(s) 142, 574
BseGI GGATG 5 cut(s) 57, 111, 138, 509, 696
BseJI GATNNNNATC 1 cut(s) 722
BseLI CCNNNNNNNGG 5 cut(s) 81, 130, 288, 706, 772
BseMII CTCAG 3 cut(s) 353, 408, 659
BseNI ACTGG 1 cut(s) 566
BseRI GAGGAG 3 cut(s) 38, 149, 697
BseSI GKGCMC 1 cut(s) 315
BseXI GCAGC 1 cut(s) 312
BshFI GGCC 4 cut(s) 89, 130, 239, 292
BshNI GGYRCC 2 cut(s) 146, 185
BsiSI CCGG 9 cut(s) 75, 98, 131, 174, 293, 315, 337, 587, 787
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 5 cut(s) 81, 130, 288, 706, 772
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 4 cut(s) 89, 130, 239, 292
Bsp1286I GDGCHC 1 cut(s) 315
Bsp143I GATC 2 cut(s) 463, 717
BspACI CCGC 4 cut(s) 24, 115, 236, 784
BspANI GGCC 4 cut(s) 89, 130, 239, 292
BspCNI CTCAG 3 cut(s) 354, 409, 660
BspLI GGNNCC 2 cut(s) 148, 187
BspMAI CTGCAG 1 cut(s) 647
BspPI GGATC 1 cut(s) 712
BspT107I GGYRCC 2 cut(s) 146, 185
BsrBI CCGCTC 1 cut(s) 117
BsrFI RCCGGY 3 cut(s) 130, 173, 336
BsrI ACTGG 1 cut(s) 566
BssAI RCCGGY 3 cut(s) 130, 173, 336
BssECI CCNNGG 2 cut(s) 142, 574
BssMI GATC 2 cut(s) 463, 717
BssNI GRCGYC 2 cut(s) 147, 186
BssSI CACGAG 1 cut(s) 286
Bst2BI CACGAG 1 cut(s) 286
Bst2UI CCWGG 1 cut(s) 576
Bst4CI ACNGT 4 cut(s) 283, 310, 450, 555
Bst6I CTCTTC 2 cut(s) 388, 785
BstACI GRCGYC 2 cut(s) 147, 186
BstAPI GCANNNNNTGC 1 cut(s) 642
BstC8I GCNNGC 2 cut(s) 87, 175
BstDEI CTNAG 4 cut(s) 362, 417, 435, 668
BstF5I GGATG 5 cut(s) 57, 111, 138, 509, 696
BstH2I RGCGCY 3 cut(s) 150, 189, 753
BstHHI GCGC 4 cut(s) 56, 149, 188, 752
BstKTI GATC 2 cut(s) 466, 720
BstMBI GATC 2 cut(s) 463, 717
BstMWI GCNNNNNNNGC 5 cut(s) 174, 183, 633, 642, 749
BstNI CCWGG 1 cut(s) 576
BstNSI RCATGY 1 cut(s) 457
BstSCI CCNGG 3 cut(s) 313, 574, 585
BstSFI CTRYAG 2 cut(s) 643, 745
BstSLI GKGCMC 1 cut(s) 315
BstV1I GCAGC 1 cut(s) 312
BstV2I GAAGAC 1 cut(s) 63
BstXI CCANNNNNNTGG 1 cut(s) 555
Bsu36I CCTNAGG 1 cut(s) 668
BsuI GTATCC 1 cut(s) 524
BsuRI GGCC 4 cut(s) 89, 130, 239, 292
BtsCI GGATG 5 cut(s) 57, 111, 138, 509, 696
BtsIMutI CAGTG 2 cut(s) 551, 573
Cac8I GCNNGC 2 cut(s) 87, 175
CaiI CAGNNNCTG 1 cut(s) 581
CfoI GCGC 4 cut(s) 56, 149, 188, 752
Cfr10I RCCGGY 3 cut(s) 130, 173, 336
Cfr13I GGNCC 1 cut(s) 238
CseI GACGC 1 cut(s) 718
Csp6I GTAC 2 cut(s) 334, 451
CviAII CATG 3 cut(s) 154, 232, 454
CviQI GTAC 2 cut(s) 334, 451
DdeI CTNAG 4 cut(s) 362, 417, 435, 668
DinI GGCGCC 2 cut(s) 148, 187
DpnI GATC 2 cut(s) 465, 719
DpnII GATC 2 cut(s) 463, 717
EaeI YGGCCR 1 cut(s) 128
Eam1104I CTCTTC 2 cut(s) 388, 785
EarI CTCTTC 2 cut(s) 388, 785
EciI GGCGGA 1 cut(s) 773
Eco57I CTGAAG 1 cut(s) 479
Eco81I CCTNAGG 1 cut(s) 668
EcoRII CCWGG 1 cut(s) 574
EgeI GGCGCC 2 cut(s) 148, 187
EheI GGCGCC 2 cut(s) 148, 187
FaeI CATG 3 cut(s) 157, 235, 457
FaiI YATR 8 cut(s) 41, 155, 233, 245, 372, 455, 540, 601
FaqI GGGAC 1 cut(s) 380
FatI CATG 3 cut(s) 153, 231, 453
FbaI TGATCA 1 cut(s) 463
Fnu4HI GCNGC 2 cut(s) 237, 301
FokI GGATG 5 cut(s) 44, 98, 125, 516, 683
Fsp4HI GCNGC 2 cut(s) 237, 301
GlaI GCGC 4 cut(s) 55, 148, 187, 751
GluI GCNGC 2 cut(s) 237, 301
HaeII RGCGCY 3 cut(s) 150, 189, 753
HaeIII GGCC 4 cut(s) 89, 130, 239, 292
HapII CCGG 9 cut(s) 75, 98, 131, 174, 293, 315, 337, 587, 787
HgaI GACGC 1 cut(s) 718
HhaI GCGC 4 cut(s) 56, 149, 188, 752
Hin1I GRCGYC 2 cut(s) 147, 186
Hin1II CATG 3 cut(s) 157, 235, 457
Hin6I GCGC 4 cut(s) 54, 147, 186, 750
HinP1I GCGC 4 cut(s) 54, 147, 186, 750
HindIII AAGCTT 1 cut(s) 437
HinfI GANTC 3 cut(s) 380, 419, 544
HpaII CCGG 9 cut(s) 75, 98, 131, 174, 293, 315, 337, 587, 787
HphI GGTGA 2 cut(s) 271, 727
Hpy166II GTNNAC 2 cut(s) 512, 571
Hpy188I TCNGA 2 cut(s) 125, 424
Hpy188III TCNNGA 3 cut(s) 286, 501, 757
Hpy8I GTNNAC 2 cut(s) 512, 571
Hpy99I CGWCG 1 cut(s) 743
HpyAV CCTTC 4 cut(s) 286, 338, 503, 763
HpyCH4III ACNGT 4 cut(s) 283, 310, 450, 555
HpyCH4V TGCA 4 cut(s) 111, 231, 636, 645
HpyF10VI GCNNNNNNNGC 5 cut(s) 174, 183, 633, 642, 749
HpyF3I CTNAG 4 cut(s) 362, 417, 435, 668
Hsp92I GRCGYC 2 cut(s) 147, 186
Hsp92II CATG 3 cut(s) 157, 235, 457
HspAI GCGC 4 cut(s) 54, 147, 186, 750
KasI GGCGCC 2 cut(s) 146, 185
KroI GCCGGC 1 cut(s) 173
KroNI GCCGGC 1 cut(s) 175
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 2 cut(s) 463, 717
LmnI GCTCC 3 cut(s) 162, 258, 578
Lsp1109I GCAGC 1 cut(s) 312
LweI GCATC 2 cut(s) 87, 120
MaeIII GTNAC 2 cut(s) 283, 373
MalI GATC 2 cut(s) 465, 719
MbiI CCGCTC 1 cut(s) 117
MboI GATC 2 cut(s) 463, 717
MboII GAAGA 4 cut(s) 63, 262, 375, 772
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 1 cut(s) 637
Mly113I GGCGCC 2 cut(s) 147, 186
MlyI GAGTC 2 cut(s) 374, 428
MmeI TCCRAC 2 cut(s) 542, 572
MroNI GCCGGC 1 cut(s) 173
MseI TTAA 2 cut(s) 33, 808
MspA1I CMGCKG 1 cut(s) 461
MspI CCGG 9 cut(s) 75, 98, 131, 174, 293, 315, 337, 587, 787
MspR9I CCNGG 3 cut(s) 315, 576, 587
MvaI CCWGG 1 cut(s) 576
MwoI GCNNNNNNNGC 5 cut(s) 174, 183, 633, 642, 749
NaeI GCCGGC 1 cut(s) 175
NarI GGCGCC 2 cut(s) 147, 186
NciI CCSGG 2 cut(s) 315, 587
NdeII GATC 2 cut(s) 463, 717
NgoMIV GCCGGC 1 cut(s) 173
NlaIII CATG 3 cut(s) 157, 235, 457
NlaIV GGNNCC 2 cut(s) 148, 187
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 1 cut(s) 283
NspI RCATGY 1 cut(s) 457
PciI ACATGT 1 cut(s) 453
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PdiI GCCGGC 1 cut(s) 175
PfeI GAWTC 1 cut(s) 544
PkrI GCNGC 2 cut(s) 238, 302
PleI GAGTC 2 cut(s) 374, 427
PluTI GGCGCC 2 cut(s) 150, 189
PpsI GAGTC 2 cut(s) 374, 427
PscI ACATGT 1 cut(s) 453
Psp6I CCWGG 1 cut(s) 574
PspGI CCWGG 1 cut(s) 574
PspN4I GGNNCC 2 cut(s) 148, 187
PspPI GGNCC 1 cut(s) 238
PstI CTGCAG 1 cut(s) 647
PstNI CAGNNNCTG 1 cut(s) 581
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 2 cut(s) 335, 452
RsaNI GTAC 2 cut(s) 334, 451
SaqAI TTAA 2 cut(s) 33, 808
SatI GCNGC 2 cut(s) 237, 301
Sau3AI GATC 2 cut(s) 463, 717
Sau96I GGNCC 1 cut(s) 238
SbfI CCTGCAGG 1 cut(s) 647
SchI GAGTC 2 cut(s) 374, 428
ScrFI CCNGG 3 cut(s) 315, 576, 587
SdaI CCTGCAGG 1 cut(s) 647
SduI GDGCHC 1 cut(s) 315
SetI ASST 8 cut(s) 225, 305, 441, 463, 517, 583, 621, 669
SfaNI GCATC 2 cut(s) 87, 120
SfcI CTRYAG 2 cut(s) 643, 745
SfoI GGCGCC 2 cut(s) 148, 187
Sse8387I CCTGCAGG 1 cut(s) 647
Sse9I AATT 1 cut(s) 637
SsiI CCGC 4 cut(s) 24, 115, 236, 784
SspDI GGCGCC 2 cut(s) 146, 185
StyD4I CCNGG 3 cut(s) 313, 574, 585
TaaI ACNGT 4 cut(s) 283, 310, 450, 555
TaqI TCGA 1 cut(s) 386
TasI AATT 1 cut(s) 637
TatI WGTACW 1 cut(s) 450
TauI GCSGC 1 cut(s) 239
TfiI GAWTC 1 cut(s) 544
Tru1I TTAA 2 cut(s) 33, 808
Tru9I TTAA 2 cut(s) 33, 808
TscAI CASTG 2 cut(s) 558, 573
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 1 cut(s) 300
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 3 cut(s) 28, 127, 169
TspGWI ACGGA 1 cut(s) 785
TspRI CASTG 2 cut(s) 558, 573
XceI RCATGY 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.