Rorug02G0032900

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007355
Physical Location & Seq
Reverse (-)
2586971 .. 2589803
2833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0032900.1

Sequence Viewer

Length: 972 bp
ATGGCCAATGCTTGGAAACGAGAGAAACCCATCAGACCCATCTTTCTTTTCTGCTCCGCCACTCTCTTCCTCATCTTCTTCCTCTTCTTCCTCAGCTTCCGCCCCTTTCAAAACCCTAGCCTCCTAAACCCTAACCCCATAATCCAAACCCATCTAAACCCAATCCGCGGCCCAATCCAACCTTTCAACTGCGATCTATGCCCCCAATCCCACCCAGTAATCGCCAACGTCGTCGAGGGCCTCCAGTACCCTTTCCTCTACTCGCTCGCCGATTTGGGGAAACTGCCGGAGAAGCCCCACAAGAACATTGTGAGAATGCTGAAGGGGAAGCCTTTCCGGCGACCCGACATTTCGGAGACGATTCAGGAGGTCTTGGGGAGGTTCAGGAGTGCAGGGAAGGCCAATGGGCTTGTAGTCGACGTCGGCGGCAATGTGGGCATTGCCAGCTTCGCCGCCGCCGTGATGGGCTTTCGGGTTTTTGCTTTCGAGCCGGTTTTGGAGAATCTGCAGAGGATTTGCGATGGGATTTACTTGAATCGTGTTAGGGATTTGGTCACTGTGTTTGAGGCCGCTGCGTCGGACCGCCTCGGCAATATTACCTTCCATAAGTTGGTTGGTCGTCTGGACAATAGTGCTGTTTCAGCCACTGGTGCAAAGTTGGCTTTTAAGTCTAATGAAGAGATAGCAGTTCAAGTAAGGACTGTCCCTCTTGATGATGTAATTCCAGAATTTGAACCTGTTCTTGTGCTCAAGATTGATGTTCAAGGCTGGGAATATCATGTGCTGAAAGGAGCTAAGAAGTTACTATCAAGAGCTGCAAGTGAAGCACCATATCTCATTTATGAGGAAGACGAGCGTTTGTTGGAAGCCAGTAATAGCAGTAAAGTAGAGATTCGAAAGTTCCTCTCTAGTGTGGGTTACCGTTACTGCACGCAGCATGGCACAGATGCACACTGCACCAAGGAGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

323

Amino Acids

36.12

Weight (kDa)

8.82

Isoelectric Point (pI)

33.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_21 PF05050 140 - 309 1.5e-19 Methyltransferase FkbM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 423
AccB7I CCANNNNNTGG 2 cut(s) 12, 610
AccI GTMKAC 1 cut(s) 417
AccII CGCG 1 cut(s) 168
AciI CCGC 9 cut(s) 57, 100, 166, 168, 426, 453, 456, 570, 583
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 728
AcuI CTGAAG 1 cut(s) 341
AcyI GRCGYC 1 cut(s) 420
AfaI GTAC 1 cut(s) 248
AfiI CCNNNNNNNGG 4 cut(s) 12, 167, 276, 610
AgsI TTSAA 6 cut(s) 110, 187, 535, 692, 734, 764
AluBI AGCT 4 cut(s) 96, 447, 794, 815
AluI AGCT 4 cut(s) 96, 447, 794, 815
Alw21I GWGCWC 1 cut(s) 750
Alw26I GTCTC 1 cut(s) 350
AlwNI CAGNNNCTG 1 cut(s) 647
AoxI GGCC 5 cut(s) 3, 169, 238, 399, 567
ApeKI GCWGC 3 cut(s) 572, 815, 934
ApoI RAATTY 1 cut(s) 728
Asp700I GAANNNNTTC 2 cut(s) 332, 738
AspS9I GGNCC 3 cut(s) 170, 238, 580
AsuII TTCGAA 1 cut(s) 895
AvaII GGWCC 1 cut(s) 580
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 855
Bbv12I GWGCWC 1 cut(s) 750
BbvCI CCTCAGC 1 cut(s) 92
BbvI GCAGC 3 cut(s) 559, 802, 946
BccI CCATC 5 cut(s) 38, 47, 159, 457, 515
BceAI ACGGC 1 cut(s) 443
BcoDI GTCTC 1 cut(s) 350
BfaI CTAG 2 cut(s) 117, 909
BfmI CTRYAG 1 cut(s) 506
BglI GCCNNNNNGGC 1 cut(s) 337
BisI GCNGC 8 cut(s) 169, 427, 453, 456, 570, 573, 816, 935
BlsI GCNGC 8 cut(s) 170, 428, 454, 457, 571, 574, 817, 936
Bme18I GGWCC 1 cut(s) 580
BmgT120I GGNCC 3 cut(s) 170, 238, 580
BmrI ACTGGG 1 cut(s) 209
BmsI GCATC 1 cut(s) 937
BmuI ACTGGG 1 cut(s) 209
BpiI GAAGAC 1 cut(s) 855
BpmI CTGGAG 1 cut(s) 227
Bpu10I CCTNAGC 1 cut(s) 92
Bpu14I TTCGAA 1 cut(s) 895
BpuEI CTTGAG 1 cut(s) 734
BsaHI GRCGYC 1 cut(s) 420
BsaJI CCNNGG 3 cut(s) 166, 586, 960
Bsc4I CCNNNNNNNGG 4 cut(s) 12, 167, 276, 610
Bse118I RCCGGY 1 cut(s) 490
Bse1I ACTGG 4 cut(s) 215, 244, 652, 870
Bse3DI GCAATG 2 cut(s) 436, 438
BseDI CCNNGG 3 cut(s) 166, 586, 960
BseLI CCNNNNNNNGG 4 cut(s) 12, 167, 276, 610
BseMI GCAATG 2 cut(s) 436, 438
BseMII CTCAG 1 cut(s) 106
BseNI ACTGG 4 cut(s) 215, 244, 652, 870
BseXI GCAGC 3 cut(s) 559, 802, 946
BseYI CCCAGC 1 cut(s) 768
BsgI GTGCAG 3 cut(s) 411, 913, 940
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 5 cut(s) 5, 171, 240, 401, 569
BsiHKAI GWGCWC 1 cut(s) 750
BsiSI CCGG 3 cut(s) 287, 337, 491
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 4 cut(s) 12, 167, 276, 610
BsmAI GTCTC 1 cut(s) 350
BsmBI CGTCTC 1 cut(s) 350
BsmFI GGGAC 1 cut(s) 689
BsmI GAATGC 1 cut(s) 321
BsnI GGCC 5 cut(s) 5, 171, 240, 401, 569
Bsp119I TTCGAA 1 cut(s) 895
Bsp1286I GDGCHC 1 cut(s) 750
Bsp143I GATC 1 cut(s) 193
BspACI CCGC 9 cut(s) 57, 100, 166, 168, 426, 453, 456, 570, 583
BspANI GGCC 5 cut(s) 5, 171, 240, 401, 569
BspCNI CTCAG 1 cut(s) 105
BspFNI CGCG 1 cut(s) 168
BspMAI CTGCAG 1 cut(s) 510
BspT104I TTCGAA 1 cut(s) 895
BsrDI GCAATG 2 cut(s) 436, 438
BsrFI RCCGGY 1 cut(s) 490
BsrI ACTGG 4 cut(s) 215, 244, 652, 870
BssAI RCCGGY 1 cut(s) 490
BssECI CCNNGG 3 cut(s) 166, 586, 960
BssMI GATC 1 cut(s) 193
BssNI GRCGYC 1 cut(s) 420
BssT1I CCWWGG 1 cut(s) 960
Bst4CI ACNGT 3 cut(s) 559, 703, 923
Bst6I CTCTTC 3 cut(s) 71, 89, 672
BstACI GRCGYC 1 cut(s) 420
BstBI TTCGAA 1 cut(s) 895
BstC8I GCNNGC 3 cut(s) 267, 445, 932
BstDEI CTNAG 2 cut(s) 92, 795
BstDSI CCRYGG 1 cut(s) 166
BstEII GGTNACC 1 cut(s) 917
BstFNI CGCG 1 cut(s) 168
BstKTI GATC 1 cut(s) 196
BstMAI GTCTC 1 cut(s) 350
BstMBI GATC 1 cut(s) 193
BstPI GGTNACC 1 cut(s) 917
BstSFI CTRYAG 1 cut(s) 506
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 3 cut(s) 559, 802, 946
BstV2I GAAGAC 1 cut(s) 855
BsuRI GGCC 5 cut(s) 5, 171, 240, 401, 569
BtgI CCRYGG 1 cut(s) 166
BtgZI GCGATG 1 cut(s) 534
BtsI GCAGTG 1 cut(s) 952
BtsIMutI CAGTG 3 cut(s) 555, 645, 952
Cac8I GCNNGC 3 cut(s) 267, 445, 932
CaiI CAGNNNCTG 1 cut(s) 647
Cfr10I RCCGGY 1 cut(s) 490
Cfr13I GGNCC 3 cut(s) 170, 238, 580
Cfr42I CCGCGG 1 cut(s) 169
CpoI CGGWCCG 1 cut(s) 580
CseI GACGC 1 cut(s) 564
Csp6I GTAC 1 cut(s) 247
CspI CGGWCCG 1 cut(s) 580
CviAII CATG 2 cut(s) 779, 938
CviQI GTAC 1 cut(s) 247
DdeI CTNAG 2 cut(s) 92, 795
DpnI GATC 1 cut(s) 195
DpnII GATC 1 cut(s) 193
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 71, 89, 672
EarI CTCTTC 3 cut(s) 71, 89, 672
EciI GGCGGA 2 cut(s) 46, 89
Eco130I CCWWGG 1 cut(s) 960
Eco47I GGWCC 1 cut(s) 580
Eco57I CTGAAG 1 cut(s) 341
Eco91I GGTNACC 1 cut(s) 917
EcoO109I RGGNCCY 1 cut(s) 238
EcoO65I GGTNACC 1 cut(s) 917
EcoT14I CCWWGG 1 cut(s) 960
ErhI CCWWGG 1 cut(s) 960
Esp3I CGTCTC 1 cut(s) 350
FaeI CATG 2 cut(s) 782, 941
FaiI YATR 7 cut(s) 140, 199, 606, 780, 832, 843, 939
FaqI GGGAC 1 cut(s) 689
FatI CATG 2 cut(s) 778, 937
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 8 cut(s) 169, 427, 453, 456, 570, 573, 816, 935
Fsp4HI GCNGC 8 cut(s) 169, 427, 453, 456, 570, 573, 816, 935
FspBI CTAG 2 cut(s) 117, 909
GluI GCNGC 8 cut(s) 169, 427, 453, 456, 570, 573, 816, 935
GsaI CCCAGC 1 cut(s) 772
GsuI CTGGAG 1 cut(s) 227
HaeIII GGCC 5 cut(s) 5, 171, 240, 401, 569
HapII CCGG 3 cut(s) 287, 337, 491
HgaI GACGC 1 cut(s) 564
Hin1I GRCGYC 1 cut(s) 420
Hin1II CATG 2 cut(s) 782, 941
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 4 cut(s) 361, 502, 535, 892
HpaII CCGG 3 cut(s) 287, 337, 491
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 3 cut(s) 35, 355, 580
Hpy188III TCNNGA 7 cut(s) 365, 385, 623, 710, 725, 751, 810
Hpy8I GTNNAC 1 cut(s) 418
Hpy99I CGWCG 5 cut(s) 233, 236, 422, 425, 580
HpyAV CCTTC 3 cut(s) 316, 391, 610
HpyCH4III ACNGT 3 cut(s) 559, 703, 923
HpyCH4IV ACGT 2 cut(s) 228, 420
HpyCH4V TGCA 7 cut(s) 392, 508, 653, 818, 930, 950, 957
HpyF3I CTNAG 2 cut(s) 92, 795
HpySE526I ACGT 2 cut(s) 228, 420
Hsp92I GRCGYC 1 cut(s) 420
Hsp92II CATG 2 cut(s) 782, 941
KspI CCGCGG 1 cut(s) 169
Kzo9I GATC 1 cut(s) 193
LmnI GCTCC 2 cut(s) 59, 791
Lsp1109I GCAGC 3 cut(s) 559, 802, 946
LweI GCATC 1 cut(s) 937
MaeI CTAG 2 cut(s) 117, 909
MaeII ACGT 2 cut(s) 228, 420
MaeIII GTNAC 4 cut(s) 553, 801, 917, 923
MalI GATC 1 cut(s) 195
MboI GATC 1 cut(s) 193
MboII GAAGA 7 cut(s) 58, 67, 70, 76, 79, 689, 860
MhlI GDGCHC 1 cut(s) 750
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 720, 728
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 3 cut(s) 202, 558, 843
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 332, 738
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 666, 970
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 168, 572
MspI CCGG 3 cut(s) 287, 337, 491
Mva1269I GAATGC 1 cut(s) 321
MvnI CGCG 1 cut(s) 168
NdeII GATC 1 cut(s) 193
NlaIII CATG 2 cut(s) 782, 941
NmeAIII GCCGAG 1 cut(s) 567
NmuCI GTSAC 1 cut(s) 553
NspV TTCGAA 1 cut(s) 895
PcsI WCGNNNNNNNCGW 2 cut(s) 228, 456
PctI GAATGC 1 cut(s) 321
PdmI GAANNNNTTC 2 cut(s) 332, 738
PfeI GAWTC 4 cut(s) 361, 502, 535, 892
PflMI CCANNNNNTGG 2 cut(s) 12, 610
PkrI GCNGC 8 cut(s) 170, 428, 454, 457, 571, 574, 817, 936
PspEI GGTNACC 1 cut(s) 917
PspFI CCCAGC 1 cut(s) 768
PspPI GGNCC 3 cut(s) 170, 238, 580
PstI CTGCAG 1 cut(s) 510
PstNI CAGNNNCTG 1 cut(s) 647
RsaI GTAC 1 cut(s) 248
RsaNI GTAC 1 cut(s) 247
Rsr2I CGGWCCG 1 cut(s) 580
RsrII CGGWCCG 1 cut(s) 580
SacII CCGCGG 1 cut(s) 169
SalI GTCGAC 1 cut(s) 416
SaqAI TTAA 2 cut(s) 666, 970
SatI GCNGC 8 cut(s) 169, 427, 453, 456, 570, 573, 816, 935
Sau3AI GATC 1 cut(s) 193
Sau96I GGNCC 3 cut(s) 170, 238, 580
SduI GDGCHC 1 cut(s) 750
SfaNI GCATC 1 cut(s) 937
SfcI CTRYAG 1 cut(s) 506
Sfr303I CCGCGG 1 cut(s) 169
SfuI TTCGAA 1 cut(s) 895
SgrBI CCGCGG 1 cut(s) 169
SinI GGWCC 1 cut(s) 580
SmlI CTYRAG 1 cut(s) 749
SmoI CTYRAG 1 cut(s) 749
Sse9I AATT 2 cut(s) 720, 728
SsiI CCGC 9 cut(s) 57, 100, 166, 168, 426, 453, 456, 570, 583
SspI AATATT 1 cut(s) 595
SspMI CTAG 2 cut(s) 117, 909
StyI CCWWGG 1 cut(s) 960
TaaI ACNGT 3 cut(s) 559, 703, 923
TaiI ACGT 2 cut(s) 231, 423
TaqI TCGA 4 cut(s) 234, 417, 486, 895
TasI AATT 2 cut(s) 720, 728
TauI GCSGC 5 cut(s) 171, 429, 455, 458, 572
TfiI GAWTC 4 cut(s) 361, 502, 535, 892
Tru1I TTAA 2 cut(s) 666, 970
Tru9I TTAA 2 cut(s) 666, 970
TscAI CASTG 3 cut(s) 562, 652, 959
TseFI GTSAC 1 cut(s) 553
TseI GCWGC 3 cut(s) 572, 815, 934
Tsp45I GTSAC 1 cut(s) 553
TspDTI ATGAA 1 cut(s) 690
TspRI CASTG 3 cut(s) 562, 652, 959
Van91I CCANNNNNTGG 2 cut(s) 12, 610
VpaK11BI GGWCC 1 cut(s) 580
XapI RAATTY 1 cut(s) 728
XcmI CCANNNNNNNNNTGG 1 cut(s) 611
XmiI GTMKAC 1 cut(s) 417
XmnI GAANNNNTTC 2 cut(s) 332, 738
XspI CTAG 2 cut(s) 117, 909
ZraI GACGTC 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.