Rw2G006800

ubiE/COQ5 methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0007355
Physical Location & Seq
Forward (+)
6598610 .. 6601474
2865 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G006800.1

Sequence Viewer

Length: 954 bp
ATGCCCAAATGTGTTGGAATTGGACTCGGGCTTCCTCATCGACTTCCATATTTGGCGAGTGTTTACTCTTGTTCTTCTTCTTATTCAACCACTAGTGCAGTTGGGTCTGTCCCTAATCTTGCAAAAGTAGTGAGCAAATGGAACAGTGGGGGTAGAGCACCTCGGCGTCTGGTTTTGGGCCTTGCAGCTTCATTTTGGGCTCAGTTGATGAACATGTCTGGTGCTATTGGCGGCAAGGCTTTCACTGCCTCTGCCAGGTTGAAGAGTGGTCCTTCCTTTGAAGAGATATTGAAGAATGTGGATTGGCCGGAGCAGATTCCTTACAAGCCGGAGGATTTCCAGCGCTATGATGAGTCACCAGATACATTGTTTTATGAGAGTCCGCGCTTTGTGACACATATTGATGATCCAGCCATTGCGGCACTGACTAAGTACTATTCTAAGGTGTTTCCGCCTAGCAACACTCCAGGAGTAAGCATGCTGGACATGTGTAGCAGTTGGGTTAGTCACTTTCCACCAGGATACAAGCAAGAGCGAATAGTTGGAATGGGAATGAATGAAGCAGAGCTAAAGGGGAATCCGGTTTTGACAGAGTATGTTCTTCAAGACTTGAATGTCAACCCTAAACTTCCCTTTGAAGATAATTCTTTTGGTGTGGTAACTAATGTGGTTAGTGTTGATTATATAAACAAGCCTATTGAAGTATTCAAGGAAATGTGCCGGATTCTTAAGCCAGGGGGACTTGCTATAATTAGCTTTTCCAACCGATGTTTCTGGACTAAAGCAATCTCAATCTGGACATCAACTGGTGATGCTGATCATGGTTTGATTGTTGGGTCATATTTCCACTACGCTGGAGGATTTGAACCTCCTCAGGGGGTGGATATTTCTCCCAACCCTGGACGCTCAGACCCCATGTACATTGTATACTCTAGAAAGTTATCTTCCGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

317

Amino Acids

34.83

Weight (kDa)

7.61

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 191 - 251 1.9e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 614
AccI GTMKAC 1 cut(s) 927
AccII CGCG 1 cut(s) 385
AciI CCGC 4 cut(s) 231, 383, 419, 452
AclWI GGATC 1 cut(s) 401
AcoI YGGCCR 1 cut(s) 305
AcyI GRCGYC 1 cut(s) 166
AfaI GTAC 2 cut(s) 434, 920
AfeI AGCGCT 1 cut(s) 344
AfiI CCNNNNNNNGG 3 cut(s) 255, 875, 899
AflII CTTAAG 1 cut(s) 728
AflIII ACRYGT 2 cut(s) 213, 486
AhlI ACTAGT 1 cut(s) 92
AjnI CCWGG 5 cut(s) 254, 466, 517, 733, 898
AjuI GAANNNNNNNTTGG 2 cut(s) 755, 787
AluBI AGCT 3 cut(s) 188, 568, 756
AluI AGCT 3 cut(s) 188, 568, 756
Alw21I GWGCWC 1 cut(s) 160
AlwI GGATC 1 cut(s) 401
Ama87I CYCGRG 1 cut(s) 26
Aor51HI AGCGCT 1 cut(s) 344
AoxI GGCC 2 cut(s) 178, 305
ApeKI GCWGC 1 cut(s) 185
AspLEI GCGC 2 cut(s) 345, 387
AspS9I GGNCC 2 cut(s) 178, 269
AsuHPI GGTGA 2 cut(s) 348, 821
AvaI CYCGRG 1 cut(s) 26
AvaII GGWCC 1 cut(s) 269
AxyI CCTNAGG 1 cut(s) 873
BanII GRGCYC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 160
BbvI GCAGC 1 cut(s) 197
BciT130I CCWGG 5 cut(s) 256, 468, 519, 735, 900
BciVI GTATCC 1 cut(s) 515
BclI TGATCA 1 cut(s) 817
BcuI ACTAGT 1 cut(s) 92
BfaI CTAG 3 cut(s) 93, 456, 933
BfoI RGCGCY 1 cut(s) 346
BfrI CTTAAG 1 cut(s) 728
BfuI GTATCC 1 cut(s) 515
BglI GCCNNNNNGGC 1 cut(s) 419
BisI GCNGC 3 cut(s) 186, 232, 420
BlsI GCNGC 3 cut(s) 187, 233, 421
BmcAI AGTACT 1 cut(s) 434
Bme1390I CCNGG 5 cut(s) 256, 468, 519, 735, 900
Bme18I GGWCC 1 cut(s) 269
BmeT110I CYCGRG 1 cut(s) 26
BmgT120I GGNCC 2 cut(s) 178, 269
BmrFI CCNGG 5 cut(s) 256, 468, 519, 735, 900
BmsI GCATC 1 cut(s) 802
BpmI CTGGAG 2 cut(s) 450, 876
BsaBI GATNNNNATC 1 cut(s) 816
BsaHI GRCGYC 1 cut(s) 166
BsaJI CCNNGG 3 cut(s) 161, 734, 898
BsaWI WCCGGW 1 cut(s) 580
Bsc4I CCNNNNNNNGG 3 cut(s) 255, 875, 899
Bse1I ACTGG 1 cut(s) 811
Bse21I CCTNAGG 1 cut(s) 873
Bse3DI GCAATG 1 cut(s) 414
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 5 cut(s) 256, 468, 519, 735, 900
BseDI CCNNGG 3 cut(s) 161, 734, 898
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 3 cut(s) 255, 875, 899
BseMI GCAATG 1 cut(s) 414
BseMII CTCAG 3 cut(s) 215, 887, 921
BseNI ACTGG 1 cut(s) 811
BseRI GAGGAG 1 cut(s) 861
BseXI GCAGC 1 cut(s) 197
BsgI GTGCAG 1 cut(s) 117
Bsh1236I CGCG 1 cut(s) 385
BshFI GGCC 2 cut(s) 180, 307
BsiHKAI GWGCWC 1 cut(s) 160
BsiHKCI CYCGRG 1 cut(s) 26
BsiSI CCGG 4 cut(s) 308, 329, 581, 721
BslFI GGGAC 2 cut(s) 95, 753
BslI CCNNNNNNNGG 3 cut(s) 255, 875, 899
BsmFI GGGAC 2 cut(s) 95, 753
BsnI GGCC 2 cut(s) 180, 307
BsoBI CYCGRG 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 160, 202
Bsp1407I TGTACA 1 cut(s) 918
Bsp143I GATC 2 cut(s) 406, 817
BspACI CCGC 4 cut(s) 231, 383, 419, 452
BspANI GGCC 2 cut(s) 180, 307
BspCNI CTCAG 3 cut(s) 214, 886, 920
BspFNI CGCG 1 cut(s) 385
BspPI GGATC 1 cut(s) 401
BspTI CTTAAG 1 cut(s) 728
BsrDI GCAATG 1 cut(s) 414
BsrGI TGTACA 1 cut(s) 918
BsrI ACTGG 1 cut(s) 811
BssECI CCNNGG 3 cut(s) 161, 734, 898
BssMI GATC 2 cut(s) 406, 817
BssNAI GTATAC 1 cut(s) 928
BssNI GRCGYC 1 cut(s) 166
Bst1107I GTATAC 1 cut(s) 928
Bst2UI CCWGG 5 cut(s) 256, 468, 519, 735, 900
Bst4CI ACNGT 1 cut(s) 146
Bst6I CTCTTC 2 cut(s) 257, 276
BstACI GRCGYC 1 cut(s) 166
BstAFI CTTAAG 1 cut(s) 728
BstAUI TGTACA 1 cut(s) 918
BstC8I GCNNGC 1 cut(s) 479
BstDEI CTNAG 5 cut(s) 201, 429, 441, 873, 907
BstENI CCTNNNNNAGG 1 cut(s) 253
BstFNI CGCG 1 cut(s) 385
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 2 cut(s) 345, 387
BstKTI GATC 2 cut(s) 409, 820
BstMBI GATC 2 cut(s) 406, 817
BstMWI GCNNNNNNNGC 2 cut(s) 245, 419
BstNI CCWGG 5 cut(s) 256, 468, 519, 735, 900
BstNSI RCATGY 3 cut(s) 217, 481, 490
BstSCI CCNGG 5 cut(s) 254, 466, 517, 733, 898
BstUI CGCG 1 cut(s) 385
BstV1I GCAGC 1 cut(s) 197
BstXI CCANNNNNNTGG 1 cut(s) 854
BstZ17I GTATAC 1 cut(s) 928
Bsu36I CCTNAGG 1 cut(s) 873
BsuI GTATCC 1 cut(s) 515
BsuRI GGCC 2 cut(s) 180, 307
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 3 cut(s) 151, 243, 422
Cac8I GCNNGC 1 cut(s) 479
CfoI GCGC 2 cut(s) 345, 387
Cfr13I GGNCC 2 cut(s) 178, 269
CseI GACGC 2 cut(s) 155, 912
Csp6I GTAC 2 cut(s) 433, 919
CviAII CATG 5 cut(s) 214, 478, 487, 821, 916
CviQI GTAC 2 cut(s) 433, 919
DdeI CTNAG 5 cut(s) 201, 429, 441, 873, 907
DpnI GATC 2 cut(s) 408, 819
DpnII GATC 2 cut(s) 406, 817
DrdI GACNNNNNNGTC 1 cut(s) 614
DseDI GACNNNNNNGTC 1 cut(s) 614
EaeI YGGCCR 1 cut(s) 305
Eam1104I CTCTTC 2 cut(s) 257, 276
EarI CTCTTC 2 cut(s) 257, 276
EciI GGCGGA 1 cut(s) 441
Eco24I GRGCYC 1 cut(s) 202
Eco47I GGWCC 1 cut(s) 269
Eco47III AGCGCT 1 cut(s) 344
Eco81I CCTNAGG 1 cut(s) 873
Eco88I CYCGRG 1 cut(s) 26
EcoNI CCTNNNNNAGG 1 cut(s) 253
EcoRII CCWGG 5 cut(s) 254, 466, 517, 733, 898
EcoT38I GRGCYC 1 cut(s) 202
FaeI CATG 5 cut(s) 217, 481, 490, 824, 919
FaqI GGGAC 2 cut(s) 95, 753
FatI CATG 5 cut(s) 213, 477, 486, 820, 915
FbaI TGATCA 1 cut(s) 817
FblI GTMKAC 1 cut(s) 927
Fnu4HI GCNGC 3 cut(s) 186, 232, 420
FriOI GRGCYC 1 cut(s) 202
Fsp4HI GCNGC 3 cut(s) 186, 232, 420
FspBI CTAG 3 cut(s) 93, 456, 933
GlaI GCGC 2 cut(s) 344, 386
GluI GCNGC 3 cut(s) 186, 232, 420
GsuI CTGGAG 2 cut(s) 450, 876
HaeII RGCGCY 1 cut(s) 346
HaeIII GGCC 2 cut(s) 180, 307
HapII CCGG 4 cut(s) 308, 329, 581, 721
HgaI GACGC 2 cut(s) 155, 912
HhaI GCGC 2 cut(s) 345, 387
Hin1I GRCGYC 1 cut(s) 166
Hin1II CATG 5 cut(s) 217, 481, 490, 824, 919
Hin6I GCGC 2 cut(s) 343, 385
HinP1I GCGC 2 cut(s) 343, 385
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HinfI GANTC 6 cut(s) 24, 316, 353, 379, 577, 724
HpaII CCGG 4 cut(s) 308, 329, 581, 721
HphI GGTGA 2 cut(s) 348, 821
Hpy166II GTNNAC 3 cut(s) 64, 619, 928
Hpy188I TCNGA 1 cut(s) 910
Hpy188III TCNNGA 4 cut(s) 605, 775, 796, 933
Hpy8I GTNNAC 3 cut(s) 64, 619, 928
HpyAV CCTTC 1 cut(s) 282
HpyCH4III ACNGT 1 cut(s) 146
HpyCH4V TGCA 3 cut(s) 98, 122, 185
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 419
HpyF3I CTNAG 5 cut(s) 201, 429, 441, 873, 907
Hsp92I GRCGYC 1 cut(s) 166
Hsp92II CATG 5 cut(s) 217, 481, 490, 824, 919
HspAI GCGC 2 cut(s) 343, 385
Ksp22I TGATCA 1 cut(s) 817
Kzo9I GATC 2 cut(s) 406, 817
LmnI GCTCC 1 cut(s) 310
Lsp1109I GCAGC 1 cut(s) 197
LweI GCATC 1 cut(s) 802
MaeI CTAG 3 cut(s) 93, 456, 933
MaeIII GTNAC 4 cut(s) 354, 391, 506, 658
MalI GATC 2 cut(s) 408, 819
MboI GATC 2 cut(s) 406, 817
MboII GAAGA 8 cut(s) 66, 69, 274, 293, 304, 593, 650, 936
MhlI GDGCHC 2 cut(s) 160, 202
MluCI AATT 3 cut(s) 18, 643, 750
MlyI GAGTC 3 cut(s) 18, 362, 388
MmeI TCCRAC 2 cut(s) 523, 786
MnlI CCTC 7 cut(s) 45, 171, 259, 325, 851, 879, 882
MseI TTAA 1 cut(s) 729
MslI CAYNNNNRTG 1 cut(s) 402
MspCI CTTAAG 1 cut(s) 728
MspI CCGG 4 cut(s) 308, 329, 581, 721
MspR9I CCNGG 5 cut(s) 256, 468, 519, 735, 900
MvaI CCWGG 5 cut(s) 256, 468, 519, 735, 900
MvnI CGCG 1 cut(s) 385
MwoI GCNNNNNNNGC 2 cut(s) 245, 419
NdeII GATC 2 cut(s) 406, 817
NlaIII CATG 5 cut(s) 217, 481, 490, 824, 919
NmeAIII GCCGAG 1 cut(s) 142
NmuCI GTSAC 3 cut(s) 354, 391, 506
NspI RCATGY 3 cut(s) 217, 481, 490
PaeI GCATGC 1 cut(s) 481
PciI ACATGT 2 cut(s) 213, 486
PfeI GAWTC 3 cut(s) 316, 577, 724
PfoI TCCNGGA 1 cut(s) 466
PkrI GCNGC 3 cut(s) 187, 233, 421
PleI GAGTC 3 cut(s) 18, 361, 387
PpsI GAGTC 3 cut(s) 18, 361, 387
PscI ACATGT 2 cut(s) 213, 486
Psp6I CCWGG 5 cut(s) 254, 466, 517, 733, 898
PspGI CCWGG 5 cut(s) 254, 466, 517, 733, 898
PspPI GGNCC 2 cut(s) 178, 269
RsaI GTAC 2 cut(s) 434, 920
RsaNI GTAC 2 cut(s) 433, 919
RseI CAYNNNNRTG 1 cut(s) 402
SaqAI TTAA 1 cut(s) 729
SatI GCNGC 3 cut(s) 186, 232, 420
Sau3AI GATC 2 cut(s) 406, 817
Sau96I GGNCC 2 cut(s) 178, 269
ScaI AGTACT 1 cut(s) 434
SchI GAGTC 3 cut(s) 18, 362, 388
ScrFI CCNGG 5 cut(s) 256, 468, 519, 735, 900
SduI GDGCHC 2 cut(s) 160, 202
SetI ASST 7 cut(s) 163, 190, 260, 447, 570, 758, 871
SfaNI GCATC 1 cut(s) 802
SinI GGWCC 1 cut(s) 269
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 1 cut(s) 728
SmoI CTYRAG 1 cut(s) 728
SpeI ACTAGT 1 cut(s) 92
SphI GCATGC 1 cut(s) 481
Sse9I AATT 3 cut(s) 18, 643, 750
SsiI CCGC 4 cut(s) 231, 383, 419, 452
SspMI CTAG 3 cut(s) 93, 456, 933
StyD4I CCNGG 5 cut(s) 254, 466, 517, 733, 898
TaaI ACNGT 1 cut(s) 146
TaqI TCGA 1 cut(s) 40
TasI AATT 3 cut(s) 18, 643, 750
TatI WGTACW 2 cut(s) 432, 918
TauI GCSGC 2 cut(s) 234, 422
TfiI GAWTC 3 cut(s) 316, 577, 724
Tru1I TTAA 1 cut(s) 729
Tru9I TTAA 1 cut(s) 729
TscAI CASTG 3 cut(s) 151, 250, 429
TseFI GTSAC 3 cut(s) 354, 391, 506
TseI GCWGC 1 cut(s) 185
Tsp45I GTSAC 3 cut(s) 354, 391, 506
TspDTI ATGAA 4 cut(s) 180, 224, 569, 573
TspGWI ACGGA 1 cut(s) 937
TspRI CASTG 3 cut(s) 151, 250, 429
Vha464I CTTAAG 1 cut(s) 728
VpaK11BI GGWCC 1 cut(s) 269
XagI CCTNNNNNAGG 1 cut(s) 253
XbaI TCTAGA 1 cut(s) 932
XceI RCATGY 3 cut(s) 217, 481, 490
XmiI GTMKAC 1 cut(s) 927
XspI CTAG 3 cut(s) 93, 456, 933
ZrmI AGTACT 1 cut(s) 434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.