Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0010745

Physical Location & Seq

Genomic Coordinates Forward (+)

6740821 .. 6744778

Gene Structure 3958 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1085800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3360 bp

ATGTCAAATTTGGAAGGTGGGAAGAAGAGGGGCGGAGATTCCGGTAATCGGAAATTGTTGAATGAGATTGAGAGTATAAGCAAGGCTTTATATGTAGACAAAAAATCCTCTAAGAGTTCAATTCCGGCGGGGAGTAAACCGTCAATGTCTCTAGGGAAGAACCGTTTACCTGATCCGAAATCGAAGAATAAGTCTGGTGGTGAAAACTTGTTAAACAAAGAGAAGAGGTCCTTTTGGAATTGGAAGCCCCTGAAGGCGATTACACATATCCGAAACCGTAGGTTTAACTGCTGCTTTTCTCTTCAAGTCCATTCCATTGAAGGGTTGCCCTCCACTTTGAATGGAATTAGTCTCTGTGTACATTGGAAGAGGCGGGATGGGATTTTCGTTACTAATCCAGTGAAGGTTGTTCAAGGGACAGCAAAATTTGAGGAGAAGTTGGCTCACACTTGCTCAGTGTATGGTAGTAGGAGTGGACCTCACCATTCGGCAAAGTATGAAGCGAAGCATTTTTTGCTATATGCTTCTGTGTTTGATGCACCAGAACTTGATTTGGGGAAGCATAGGATTGATCTCACGAGGCTGCTTCCTCTGACATTGGAGGAGTTAGAGGAGGAGAAAAGCTCGAGGAACTGGACGACGAGTTTCAAATTATCAGGAAAGGCTAAAGGTGGTTCATTGAATGTTAGTTTTGGGTATACAGTGCTTGAAGATAATCCCAGAGCAACAGGAAATAGCCAGAATGTTCCTGAGGCATTGATTTCGAGGCAGAATAACTCAAGTATAAAGCGTGCTGGAACTCTTCCTAATCAGCAGTCACGGGCCTCATCTCAATCCGTACAGGGCATAAAGGATCTTCATGGGGTTTTGCCAGTATCAAGGTCGGAACTTTCTAGTTCAGTGAATACACTATTTCAGAAATTTGACGAAGAAGAGAAGTCAGATACTCCAGTTGACAAGCATCTTGATCCTATGAAGACGACTTTTTTCCCATCATCTGGTTCTGGTAAAGAAGTAGAAAATGAGTGTGAAGAAAACGAATTTTCCGTAGTTGAACAGGGGATAGAATTACCATCAAAGGAACTGGCGGAATCAGAAGTTGTTACACAGGTTGCTGATGCTTCTCCAGCAGAAAGCCATTTTTCTGAAATCACCACTGGTGTACAAGTAGCAGTTGAAGATGAAGCTGAGCTCGAATCCCAGGCTGGGGAAAAGGGTCATACTGATGACCTTATGGTAAGTGAGTCCACTTCCAACAGAGATGCCTTGTGCACCAAAGAATCACTCATGAAAGAGCTAGAGTCCGCTCTAAGTATTGTATCCGATTTGGAGAGTGCAGCACTAGAATCTTCACCTGAAGACCAAAGATGTTATGTGGAAGGTAAATTAGATTCAAAACTAAATATGATGGGGAGATCGCATAGCTTGGATGATGTTACAGAATCTGTTGAAAACGAGTTCTTGAACATGCTAGGGATAGATCATAGTCCAATTTCCTTGAGTTCTGAGAGTGATCCCGAGTCTCCAAGAGAGCGTCTGCTAAGACAATTTGAGAAGGAAGCCCTTGCTGGAGGTACTTCTTTGTTTGATTTTGACGCAGGCATCAGTGACCAAACAGACTATGGATACACGCCTTCAACTGAGACTGGTTGGGAGAACTTATCTGATAGTTTTGATTTCTCATCTGTGATTCAAGCTGCAGAGGAAGAGCATCAGATAGCAGCTCAGGCAGTAAAAAGTAAAGCAAAAGCCAAAATGTTGGAGGACTTGGAGACGGAAGCATTAATGCGGGAGTGGGGCTTAAATGAGATGGCCTTTCAGCATTCTCCACCAAAAGGTTGTGCTAGTTTTGGAAGTTCGATGGATTTGCCTGCTGAAGAACCTTTAGAATTGCCTCCTCTTGGAGAAGGGTTAGGTCCTTTTCTTCAGACAAAGAATGGAGGGTTTGTGCGGTCCATGAATCCCTCACTTTTCAGCGAGGCCAAAAGTGGCGGGAACTTGATCATGCAGGTTTCTAGTCCAGTTGTGGTACCGGCAGAAATGGGTTCTGGGGTAATGGAGATATTGCAGCATTTGGCATCAGTAGGAATTGAGAAGCTTTCTATGCAGGCAAATAAGCTAATGCCCTTGGAAGATATCACTGGAAAGACAATGGAACAAGTAGCATGGGAAGCTGTGCCTGCTTTGGAAGGACCTCAGAGGGAATTTGTGGCGCAGCATGAATCAGTTGGGTATCATACATCGGATGGGCTAACAAGAGCTAAAGGAATTTCATCTGGGCCTAAGTCTAATAAGTTAAGTTCGAGTGCAGCTGGCAACGAGATGGGCTTAGAATATGTCTCTTTAGAAGATCTTGCTCCTTTAGCCATGGATAAAATTGAAGCACTCTCAATTGAGGGGTTGAGAATACAAGCCGGAATGTCCAATGCAGATGCACCTTCAAATATCAGCGCACAGTCTGTTGCGGAAATCTCAGCTCTCCAGGGCAAGGGTGTCAACGTTGGTGAATCCCTTGGTCTGGAAGGAGCCGCTGGGATGCAGTTGTTAGACATAAAAGACAGTGGCAATGATGTTGACGGATTAATGGGCCTCTCATTAACTCTTGATGAATGGTTGAAACTCGATTCTGGTGAAATTGATGATGGTGATCATATTAGTGAACGGACTTCTCAAATCCTTGCGGCTCATCATGCAAACTCTTTGGACATGATTCGCGGAGGGTCAAAGGGAGAGAGAAGGCGTGGCAAAGGAGCTAGAAAGTGTGGCTTGTTGGGGAACAATTTCACAGTAGCACTGATGGTGCAACTTCGTGATCCACTACGAAACTATGAACCTGTTGGGGCACCAATGCTTTCTCTTATTCAAGTAGAAAGAGTGTTTCTCCCACCGAAGCCAAGAATATACATCTCGGTTTCAGAGCTGAGGAAGAATGATGAAGAGAATGATCAGTCTGAGTCAGTGGGAAAAGAAGAAGAAATAAAGGAGGAGATGAAAGATGAGAAATTGGCCGAAGTGGAAGCTATTCCTAAGTTTAGAATCACTGAAGTCCATGTTGCAGGTCTGAAAACCGAGCCAGATAAAAAGAAGCCATGGGGTACTGCAAACCAGAAACAGTCCGGTTCCCGCTGGTTGCTTGCTAATGGAATGGGGAAGAGCAATAAGCATCCGTTTATGAAGTCAAAAGCTGCTCCAAAATCTTCTGGTCCAGCAACAACAAAGGTTCAGCCTGGTGATACCTTGTGGAGCATCTCAGCACGCGTTCATGGTACTGGAGAAAAATGGAAGGAATTGGCAGCACTAAATCCACATATAAGGAACCCCAATGTTATTTTTCCGAATGAAACTCTCAGATTAAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006810 GO:0006928 GO:0006996 GO:0007097 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009657 GO:0009658 GO:0009902 GO:0009903 GO:0009987 GO:0016043 GO:0019750 GO:0030029 GO:0030048 GO:0030705 GO:0031022 GO:0046907 GO:0050896 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051644 GO:0051647 GO:0051649 GO:0051656 GO:0051667 GO:0071840 GO:0099515

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LysM NT-C2

Protein Analysis

1120

Amino Acids

121.85

Weight (kDa)

5.3

Isoelectric Point (pI)

46.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NT-C2	PF10358	92 - 240	1.3e-18	N-terminal C2 in EEIG1 and EHBP1 proteins
PMI1_PMIR1-2_C	PF21745	666 - 817	2.2e-60	PMI1/PMIR1-2, C-terminal domain
LysM	PF01476	1073 - 1118	8.3e-10	LysM domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010745)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20610
fragaria_vesca	FvH4_1g08130
malus_domestica	MD02G1085800.v1.1 MD15G1213000.v1.1
prunus_persica	Prupe.7G203900_v2.0.a1
pyrus_communis	pycom02g06870 pycom02g06880 pycom15g18930 pycom15g18940
rosa_chinensis	RchiOBHm_Chr2g0094181
rosa_laevigata	RLG00000016463
rosa_multiflora	Rmu_sc0002193.1_g000003 Rmu_sc0013176.1_g000002
rosa_roxburghii	Rroxscaffold_2G00147410
rosa_rugosa	Rorug02G0041600 Rorug02G0041700
rosa_samantha	Rh2AG089300 Rh2BG089400 Rh2CG091900 Rh2DG088400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	1999, 3047
Acc65I	GGTACC	1 cut(s)	2029
AccB1I	GGYRCC	2 cut(s)	2029, 2842
AccB7I	CCANNNNNTGG	1 cut(s)	998
AccBSI	CCGCTC	1 cut(s)	1307
AccI	GTMKAC	2 cut(s)	96, 698
AccII	CGCG	2 cut(s)	2715, 3258
AclI	AACGTT	1 cut(s)	2499
AclWI	GGATC	5 cut(s)	167, 861, 962, 1508, 2807
AcoI	YGGCCR	1 cut(s)	3006
AcsI	RAATTY	6 cut(s)	7, 425, 920, 1040, 2204, 2268
AcuI	CTGAAG	5 cut(s)	272, 1377, 1896, 1910, 3063
AfaI	GTAC	7 cut(s)	360, 840, 1164, 1576, 2031, 3097, 3268
AfiI	CCNNNNNNNGG	9 cut(s)	48, 321, 998, 1206, 1207, 1835, 1901, 2488, 2518
AflIII	ACRYGT	1 cut(s)	3256
AjnI	CCWGG	3 cut(s)	1200, 2481, 3226
Alw21I	GWGCWC	2 cut(s)	1194, 1274
Alw26I	GTCTC	6 cut(s)	153, 356, 1527, 1637, 1766, 2344
Alw44I	GTGCAC	1 cut(s)	1270
AlwI	GGATC	5 cut(s)	167, 861, 962, 1508, 2807
AlwNI	CAGNNNCTG	1 cut(s)	1445
Ama87I	CYCGRG	2 cut(s)	625, 1517
AoxI	GGCC	6 cut(s)	822, 1812, 1980, 2279, 2587, 3006
ApaLI	GTGCAC	1 cut(s)	1270
ApoI	RAATTY	6 cut(s)	7, 425, 920, 1040, 2204, 2268
ArsI	GACNNNNNNTTYG	2 cut(s)	176, 208
AseI	ATTAAT	2 cut(s)	1784, 2582
Asp700I	GAANNNNTTC	2 cut(s)	2780, 3021
Asp718I	GGTACC	1 cut(s)	2029
AspLEI	GCGC	2 cut(s)	2215, 2453
AspS9I	GGNCC	9 cut(s)	228, 476, 822, 1916, 1953, 2192, 2279, 2587, 3203
AsuHPI	GGTGA	8 cut(s)	212, 473, 1144, 1344, 2516, 2642, 2657, 3242
AvaI	CYCGRG	2 cut(s)	625, 1517
AvaII	GGWCC	6 cut(s)	228, 476, 1916, 1953, 2192, 3203
AxyI	CCTNAGG	1 cut(s)	750
BaeGI	GKGCMC	2 cut(s)	1274, 2845
BaeI	ACNNNNGTAYC	2 cut(s)	936, 969
BanI	GGYRCC	2 cut(s)	2029, 2842
BanII	GRGCYC	1 cut(s)	1194
BauI	CACGAG	1 cut(s)	577
BbsI	GAAGAC	2 cut(s)	983, 1365
Bbv12I	GWGCWC	2 cut(s)	1194, 1274
BbvCI	CCTCAGC	1 cut(s)	2921
BcgI	CGANNNNNNTGC	4 cut(s)	744, 778, 1849, 1883
BciT130I	CCWGG	3 cut(s)	1202, 2483, 3228
BciVI	GTATCC	2 cut(s)	1330, 1619
BclI	TGATCA	3 cut(s)	2001, 2647, 2944
BcoDI	GTCTC	6 cut(s)	153, 356, 1527, 1637, 1766, 2344
BfaI	CTAG	8 cut(s)	152, 894, 1298, 1343, 1472, 1845, 2016, 2754
BfmI	CTRYAG	1 cut(s)	1698
BfuAI	ACCTGC	2 cut(s)	1999, 3047
BfuI	GTATCC	2 cut(s)	1330, 1619
BglII	AGATCT	1 cut(s)	2350
BlpI	GCTNAGC	1 cut(s)	1188
Bme1390I	CCNGG	3 cut(s)	1202, 2483, 3228
Bme18I	GGWCC	6 cut(s)	228, 476, 1916, 1953, 2192, 3203
BmeT110I	CYCGRG	2 cut(s)	625, 1517
BmgT120I	GGNCC	9 cut(s)	228, 476, 822, 1916, 1953, 2192, 2279, 2587, 3203
BmiI	GGNNCC	5 cut(s)	2031, 2527, 2844, 3121, 3317
BmrFI	CCNGG	3 cut(s)	1202, 2483, 3228
BpiI	GAAGAC	2 cut(s)	983, 1365
BplI	GAGNNNNNCTC	6 cut(s)	463, 495, 608, 640, 2865, 2897
BpmI	CTGGAG	5 cut(s)	933, 1110, 1590, 2465, 3291
Bpu10I	CCTNAGC	2 cut(s)	1725, 2921
Bpu1102I	GCTNAGC	1 cut(s)	1188
BpuEI	CTTGAG	2 cut(s)	763, 1519
BsaBI	GATNNNNATC	1 cut(s)	2646
BsaJI	CCNNGG	6 cut(s)	1200, 2127, 2367, 2482, 2512, 3089
BsaWI	WCCGGW	2 cut(s)	41, 3116
BsaXI	ACNNNNNCTCC	2 cut(s)	1932, 1962
Bsc4I	CCNNNNNNNGG	9 cut(s)	48, 321, 998, 1206, 1207, 1835, 1901, 2488, 2518
Bse118I	RCCGGY	1 cut(s)	2032
Bse21I	CCTNAGG	1 cut(s)	750
Bse3DI	GCAATG	1 cut(s)	2572
Bse8I	GATNNNNATC	1 cut(s)	2646
BseBI	CCWGG	3 cut(s)	1202, 2483, 3228
BseDI	CCNNGG	6 cut(s)	1200, 2127, 2367, 2482, 2512, 3089
BseGI	GGATG	5 cut(s)	382, 1435, 2251, 2541, 3163
BseJI	GATNNNNATC	1 cut(s)	2646
BseLI	CCNNNNNNNGG	9 cut(s)	48, 321, 998, 1206, 1207, 1835, 1901, 2488, 2518
BseMI	GCAATG	1 cut(s)	2572
BseRI	GAGGAG	6 cut(s)	446, 617, 626, 629, 1887, 2999
BseSI	GKGCMC	2 cut(s)	1274, 2845
BseYI	CCCAGC	2 cut(s)	1205, 2531
BsgI	GTGCAG	2 cut(s)	1356, 2328
Bsh1236I	CGCG	2 cut(s)	2715, 3258
BshFI	GGCC	6 cut(s)	824, 1814, 1982, 2281, 2589, 3008
BshNI	GGYRCC	2 cut(s)	2029, 2842
BsiHKAI	GWGCWC	2 cut(s)	1194, 1274
BsiHKCI	CYCGRG	2 cut(s)	625, 1517
BsiSI	CCGG	5 cut(s)	42, 125, 2033, 2415, 3117
BslFI	GGGAC	1 cut(s)	430
BslI	CCNNNNNNNGG	9 cut(s)	48, 321, 998, 1206, 1207, 1835, 1901, 2488, 2518
BsmAI	GTCTC	6 cut(s)	153, 356, 1527, 1637, 1766, 2344
BsmBI	CGTCTC	1 cut(s)	1766
BsmFI	GGGAC	1 cut(s)	430
BsmI	GAATGC	1 cut(s)	1822
BsnI	GGCC	6 cut(s)	824, 1814, 1982, 2281, 2589, 3008
BsoBI	CYCGRG	2 cut(s)	625, 1517
Bsp1286I	GDGCHC	3 cut(s)	1194, 1274, 2845
Bsp1407I	TGTACA	2 cut(s)	358, 1162
Bsp1720I	GCTNAGC	1 cut(s)	1188
Bsp19I	CCATGG	2 cut(s)	2367, 3089
BspANI	GGCC	6 cut(s)	824, 1814, 1982, 2281, 2589, 3008
BspFNI	CGCG	2 cut(s)	2715, 3258
BspHI	TCATGA	1 cut(s)	1287
BspLI	GGNNCC	5 cut(s)	2031, 2527, 2844, 3121, 3317
BspMAI	CTGCAG	1 cut(s)	1702
BspMI	ACCTGC	2 cut(s)	1999, 3047
BspPI	GGATC	5 cut(s)	167, 861, 962, 1508, 2807
BspQI	GCTCTTC	2 cut(s)	1701, 3146
BspT107I	GGYRCC	2 cut(s)	2029, 2842
BsrBI	CCGCTC	1 cut(s)	1307
BsrDI	GCAATG	1 cut(s)	2572
BsrFI	RCCGGY	1 cut(s)	2032
BsrGI	TGTACA	2 cut(s)	358, 1162
BssAI	RCCGGY	1 cut(s)	2032
BssECI	CCNNGG	6 cut(s)	1200, 2127, 2367, 2482, 2512, 3089
BssNAI	GTATAC	1 cut(s)	699
BssSI	CACGAG	1 cut(s)	577
BssT1I	CCWWGG	4 cut(s)	2127, 2367, 2512, 3089
Bst1107I	GTATAC	1 cut(s)	699
Bst2BI	CACGAG	1 cut(s)	577
Bst2UI	CCWGG	3 cut(s)	1202, 2483, 3228
Bst4CI	ACNGT	8 cut(s)	141, 164, 278, 703, 2457, 2561, 2788, 3114
Bst6I	CTCTTC	9 cut(s)	20, 218, 306, 362, 807, 927, 1701, 2931, 3146
BstAPI	GCANNNNNTGC	1 cut(s)	514
BstAUI	TGTACA	2 cut(s)	358, 1162
BstC8I	GCNNGC	8 cut(s)	792, 1600, 1872, 2109, 2181, 2314, 3135, 3256
BstDSI	CCRYGG	2 cut(s)	2367, 3089
BstF5I	GGATG	5 cut(s)	382, 1435, 2251, 2541, 3163
BstFNI	CGCG	2 cut(s)	2715, 3258
BstHHI	GCGC	2 cut(s)	2215, 2453
BstMAI	GTCTC	6 cut(s)	153, 356, 1527, 1637, 1766, 2344
BstMWI	GCNNNNNNNGC	8 cut(s)	514, 1127, 1727, 2104, 2171, 2180, 2363, 2690
BstNI	CCWGG	3 cut(s)	1202, 2483, 3228
BstNSI	RCATGY	1 cut(s)	1471
BstSCI	CCNGG	3 cut(s)	1200, 2481, 3226
BstSFI	CTRYAG	1 cut(s)	1698
BstSLI	GKGCMC	2 cut(s)	1274, 2845
BstUI	CGCG	2 cut(s)	2715, 3258
BstV2I	GAAGAC	2 cut(s)	983, 1365
BstX2I	RGATCY	2 cut(s)	853, 2350
BstXI	CCANNNNNNTGG	1 cut(s)	1759
BstYI	RGATCY	2 cut(s)	853, 2350
BstZ17I	GTATAC	1 cut(s)	699
Bsu36I	CCTNAGG	1 cut(s)	750
BsuI	GTATCC	2 cut(s)	1330, 1619
BsuRI	GGCC	6 cut(s)	824, 1814, 1982, 2281, 2589, 3008
BtgI	CCRYGG	2 cut(s)	2367, 3089
BtsCI	GGATG	5 cut(s)	382, 1435, 2251, 2541, 3163
BveI	ACCTGC	2 cut(s)	1999, 3047
Cac8I	GCNNGC	8 cut(s)	792, 1600, 1872, 2109, 2181, 2314, 3135, 3256
CaiI	CAGNNNCTG	1 cut(s)	1445
CciI	TCATGA	1 cut(s)	1287
CfoI	GCGC	2 cut(s)	2215, 2453
Cfr10I	RCCGGY	1 cut(s)	2032
Cfr13I	GGNCC	9 cut(s)	228, 476, 822, 1916, 1953, 2192, 2279, 2587, 3203
CseI	GACGC	2 cut(s)	1523, 1604
Csp6I	GTAC	7 cut(s)	359, 839, 1163, 1575, 2030, 3096, 3267
CviQI	GTAC	7 cut(s)	359, 839, 1163, 1575, 2030, 3096, 3267
EaeI	YGGCCR	1 cut(s)	3006
Eam1104I	CTCTTC	9 cut(s)	20, 218, 306, 362, 807, 927, 1701, 2931, 3146
EarI	CTCTTC	9 cut(s)	20, 218, 306, 362, 807, 927, 1701, 2931, 3146
EciI	GGCGGA	2 cut(s)	48, 1103
Ecl136II	GAGCTC	1 cut(s)	1192
Eco130I	CCWWGG	4 cut(s)	2127, 2367, 2512, 3089
Eco24I	GRGCYC	1 cut(s)	1194
Eco32I	GATATC	1 cut(s)	2137
Eco47I	GGWCC	6 cut(s)	228, 476, 1916, 1953, 2192, 3203
Eco53kI	GAGCTC	1 cut(s)	1192
Eco57I	CTGAAG	5 cut(s)	272, 1377, 1896, 1910, 3063
Eco81I	CCTNAGG	1 cut(s)	750
Eco88I	CYCGRG	2 cut(s)	625, 1517
EcoICRI	GAGCTC	1 cut(s)	1192
EcoO109I	RGGNCCY	3 cut(s)	228, 1916, 2192
EcoRII	CCWGG	3 cut(s)	1200, 2481, 3226
EcoRV	GATATC	1 cut(s)	2137
EcoT14I	CCWWGG	4 cut(s)	2127, 2367, 2512, 3089
EcoT38I	GRGCYC	1 cut(s)	1194
ErhI	CCWWGG	4 cut(s)	2127, 2367, 2512, 3089
Esp3I	CGTCTC	1 cut(s)	1766
FalI	AAGNNNNNCTT	6 cut(s)	70, 102, 215, 247, 2750, 2782
FaqI	GGGAC	1 cut(s)	430
FauI	CCCGC	5 cut(s)	121, 366, 1782, 1985, 3131
FbaI	TGATCA	3 cut(s)	2001, 2647, 2944
FblI	GTMKAC	2 cut(s)	96, 698
FokI	GGATG	5 cut(s)	389, 1442, 2258, 2548, 3150
FriOI	GRGCYC	1 cut(s)	1194
FspBI	CTAG	8 cut(s)	152, 894, 1298, 1343, 1472, 1845, 2016, 2754
GlaI	GCGC	2 cut(s)	2214, 2452
GsaI	CCCAGC	2 cut(s)	1209, 2535
GsuI	CTGGAG	5 cut(s)	933, 1110, 1590, 2465, 3291
HaeIII	GGCC	6 cut(s)	824, 1814, 1982, 2281, 2589, 3008
HapII	CCGG	5 cut(s)	42, 125, 2033, 2415, 3117
HgaI	GACGC	2 cut(s)	1523, 1604
HhaI	GCGC	2 cut(s)	2215, 2453
Hin6I	GCGC	2 cut(s)	2213, 2451
HinP1I	GCGC	2 cut(s)	2213, 2451
HincII	GTYRAC	3 cut(s)	955, 2497, 2575
HindII	GTYRAC	3 cut(s)	955, 2497, 2575
HindIII	AAGCTT	1 cut(s)	2096
HpaII	CCGG	5 cut(s)	42, 125, 2033, 2415, 3117
HphI	GGTGA	8 cut(s)	212, 473, 1144, 1344, 2516, 2642, 2657, 3242
Hpy99I	CGWCG	1 cut(s)	643
HpyCH4III	ACNGT	8 cut(s)	141, 164, 278, 703, 2457, 2561, 2788, 3114
HpyCH4IV	ACGT	1 cut(s)	2499
HpyF10VI	GCNNNNNNNGC	8 cut(s)	514, 1127, 1727, 2104, 2171, 2180, 2363, 2690
HpySE526I	ACGT	1 cut(s)	2499
HspAI	GCGC	2 cut(s)	2213, 2451
KpnI	GGTACC	1 cut(s)	2033
Ksp22I	TGATCA	3 cut(s)	2001, 2647, 2944
LguI	GCTCTTC	2 cut(s)	1701, 3146
LmnI	GCTCC	5 cut(s)	2362, 2525, 2750, 3193, 3243
MaeI	CTAG	8 cut(s)	152, 894, 1298, 1343, 1472, 1845, 2016, 2754
MaeII	ACGT	1 cut(s)	2499
MaeIII	GTNAC	5 cut(s)	388, 816, 1102, 1435, 1607
MbiI	CCGCTC	1 cut(s)	1307
MfeI	CAATTG	1 cut(s)	2391
MflI	RGATCY	2 cut(s)	853, 2350
MhlI	GDGCHC	3 cut(s)	1194, 1274, 2845
MluI	ACGCGT	1 cut(s)	3256
MlyI	GAGTC	4 cut(s)	1253, 1310, 1529, 2963
MmeI	TCCRAC	3 cut(s)	864, 1278, 1740
MroXI	GAANNNNTTC	2 cut(s)	2780, 3021
MseI	TTAA	8 cut(s)	212, 285, 1784, 1802, 2297, 2582, 2597, 3353
MslI	CAYNNNNRTG	2 cut(s)	1224, 2655
MspA1I	CMGCKG	3 cut(s)	2312, 2531, 3126
MspI	CCGG	5 cut(s)	42, 125, 2033, 2415, 3117
MspR9I	CCNGG	3 cut(s)	1202, 2483, 3228
MunI	CAATTG	1 cut(s)	2391
Mva1269I	GAATGC	1 cut(s)	1822
MvaI	CCWGG	3 cut(s)	1202, 2483, 3228
MvnI	CGCG	2 cut(s)	2715, 3258
MwoI	GCNNNNNNNGC	8 cut(s)	514, 1127, 1727, 2104, 2171, 2180, 2363, 2690
NcoI	CCATGG	2 cut(s)	2367, 3089
NlaIV	GGNNCC	5 cut(s)	2031, 2527, 2844, 3121, 3317
NmuCI	GTSAC	2 cut(s)	816, 1607
NspI	RCATGY	1 cut(s)	1471
PaeR7I	CTCGAG	1 cut(s)	625
PagI	TCATGA	1 cut(s)	1287
PciSI	GCTCTTC	2 cut(s)	1701, 3146
PcsI	WCGNNNNNNNCGW	1 cut(s)	1044
PctI	GAATGC	1 cut(s)	1822
PdmI	GAANNNNTTC	2 cut(s)	2780, 3021
PflMI	CCANNNNNTGG	1 cut(s)	998
PleI	GAGTC	4 cut(s)	1252, 1309, 1528, 2962
PpsI	GAGTC	4 cut(s)	1252, 1309, 1528, 2962
PpuMI	RGGWCCY	3 cut(s)	228, 1916, 2192
PshBI	ATTAAT	2 cut(s)	1784, 2582
Psp124BI	GAGCTC	1 cut(s)	1194
Psp1406I	AACGTT	1 cut(s)	2499
Psp5II	RGGWCCY	3 cut(s)	228, 1916, 2192
Psp6I	CCWGG	3 cut(s)	1200, 2481, 3226
PspFI	CCCAGC	2 cut(s)	1205, 2531
PspGI	CCWGG	3 cut(s)	1200, 2481, 3226
PspN4I	GGNNCC	5 cut(s)	2031, 2527, 2844, 3121, 3317
PspPI	GGNCC	9 cut(s)	228, 476, 822, 1916, 1953, 2192, 2279, 2587, 3203
PspPPI	RGGWCCY	3 cut(s)	228, 1916, 2192
PspXI	VCTCGAGB	1 cut(s)	625
PstI	CTGCAG	1 cut(s)	1702
PstNI	CAGNNNCTG	1 cut(s)	1445
PsuI	RGATCY	2 cut(s)	853, 2350
PvuII	CAGCTG	1 cut(s)	2312
RsaI	GTAC	7 cut(s)	360, 840, 1164, 1576, 2031, 3097, 3268
RsaNI	GTAC	7 cut(s)	359, 839, 1163, 1575, 2030, 3096, 3267
RseI	CAYNNNNRTG	2 cut(s)	1224, 2655
SacI	GAGCTC	1 cut(s)	1194
SapI	GCTCTTC	2 cut(s)	1701, 3146
SaqAI	TTAA	8 cut(s)	212, 285, 1784, 1802, 2297, 2582, 2597, 3353
Sau96I	GGNCC	9 cut(s)	228, 476, 822, 1916, 1953, 2192, 2279, 2587, 3203
SchI	GAGTC	4 cut(s)	1253, 1310, 1529, 2963
ScrFI	CCNGG	3 cut(s)	1202, 2483, 3228
SduI	GDGCHC	3 cut(s)	1194, 1274, 2845
SfcI	CTRYAG	1 cut(s)	1698
Sfr274I	CTCGAG	1 cut(s)	625
SinI	GGWCC	6 cut(s)	228, 476, 1916, 1953, 2192, 3203
SlaI	CTCGAG	1 cut(s)	625
SmiMI	CAYNNNNRTG	2 cut(s)	1224, 2655
SmlI	CTYRAG	3 cut(s)	625, 778, 1498
SmoI	CTYRAG	3 cut(s)	625, 778, 1498
SspMI	CTAG	8 cut(s)	152, 894, 1298, 1343, 1472, 1845, 2016, 2754
SstI	GAGCTC	1 cut(s)	1194
StyD4I	CCNGG	3 cut(s)	1200, 2481, 3226
StyI	CCWWGG	4 cut(s)	2127, 2367, 2512, 3089
TaaI	ACNGT	8 cut(s)	141, 164, 278, 703, 2457, 2561, 2788, 3114
TaiI	ACGT	1 cut(s)	2502
TaqI	TCGA	7 cut(s)	182, 626, 764, 1194, 1859, 2303, 2622
TatI	WGTACW	2 cut(s)	358, 1162
TauI	GCSGC	2 cut(s)	2531, 2684
Tru1I	TTAA	8 cut(s)	212, 285, 1784, 1802, 2297, 2582, 2597, 3353
Tru9I	TTAA	8 cut(s)	212, 285, 1784, 1802, 2297, 2582, 2597, 3353
TseFI	GTSAC	2 cut(s)	816, 1607
Tsp45I	GTSAC	2 cut(s)	816, 1607
TspGWI	ACGGA	6 cut(s)	826, 1036, 1790, 2592, 2677, 3156
Van91I	CCANNNNNTGG	1 cut(s)	998
VneI	GTGCAC	1 cut(s)	1270
VpaK11BI	GGWCC	6 cut(s)	228, 476, 1916, 1953, 2192, 3203
VspI	ATTAAT	2 cut(s)	1784, 2582
XapI	RAATTY	6 cut(s)	7, 425, 920, 1040, 2204, 2268
XceI	RCATGY	1 cut(s)	1471
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1619
XhoI	CTCGAG	1 cut(s)	625
XmiI	GTMKAC	2 cut(s)	96, 698
XmnI	GAANNNNTTC	2 cut(s)	2780, 3021
XspI	CTAG	8 cut(s)	152, 894, 1298, 1343, 1472, 1845, 2016, 2754

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.