pycom15g18940

N-terminal C2 in EEIG1 and EHBP1 proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0010745
Physical Location & Seq
Forward (+)
13434600 .. 13435250
651 bp
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UTR
Exon/CDS
Intron
pycom15g18940.1

Sequence Viewer

Length: 651 bp
ATGATTCGTGGAGGGTCAAAGGGAGAGAGAAGGCGTGGCAAAGGAGCTAGGAAGTGTGGCCTATTGGGGAACAATTTCACAGTAGCACTGATGGTGCAACTTCGTGATCCACTACGAAACTATGAACCTGTTGGGGCACCAATGCTTTCTCTTATTCAAGTGGAAAGAGTGTTTCTCCCACCGAAGCCAAGAATATACATCTCGGTTTCAGAACTGAGGAAGAATAATCAAGAGAATGAAGAGTCTGAGTCAGTGGGAAAAGAAGAAATAAAGGAGGAGATGAAAGATGAGAAATTGGCCGAAGTGGAAGCTATTGCTCAGTTTAGAATCACGGAAGTCCATGTTGCAGGTCTGAAAACCGAGCCAGATAAAAAGAAGCCATGGGGTACTGCAAACCAGAAGCAGTCCGGTTCCCGCTGGTTGCTTGCTAATGGAATGGGGAAAAGCAATAAGCATCCATTTATGAAGTCAAAAGCTGCTCCAAAATCTTCTGGTCCAGCAACGACAAAGGTGCAGCCTGGTGATACCTTGTGGAGCATCTCAGCACGCGTTCATGGCACTGGAGAAAAATGGAAGGAATTGGCGGCTCTAAATCCACATATAAGGAACCCAAACGTTATATTTCCGAATGAGACTCTCAGATTAAGCTGA

Protein Analysis

217

Amino Acids

24.07

Weight (kDa)

9.93

Isoelectric Point (pI)

38.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LysM PF01476 170 - 215 8.8e-11 LysM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 338
AccB1I GGYRCC 1 cut(s) 136
AccII CGCG 1 cut(s) 549
AciI CCGC 2 cut(s) 415, 584
AclI AACGTT 1 cut(s) 615
AclWI GGATC 1 cut(s) 101
AcoI YGGCCR 1 cut(s) 297
AfaI GTAC 1 cut(s) 388
AflIII ACRYGT 1 cut(s) 547
AgsI TTSAA 1 cut(s) 158
AjnI CCWGG 1 cut(s) 517
AluBI AGCT 4 cut(s) 47, 311, 476, 648
AluI AGCT 4 cut(s) 47, 311, 476, 648
Alw26I GTCTC 1 cut(s) 626
AlwI GGATC 1 cut(s) 101
AoxI GGCC 2 cut(s) 58, 297
ApeKI GCWGC 2 cut(s) 476, 514
Asp700I GAANNNNTTC 1 cut(s) 74
AspS9I GGNCC 1 cut(s) 494
AsuHPI GGTGA 1 cut(s) 533
AvaII GGWCC 1 cut(s) 494
BaeGI GKGCMC 1 cut(s) 139
BanI GGYRCC 1 cut(s) 136
BbvI GCAGC 2 cut(s) 463, 526
BccI CCATC 1 cut(s) 85
BcgI CGANNNNNNTGC 2 cut(s) 493, 527
BciT130I CCWGG 1 cut(s) 519
BcoDI GTCTC 1 cut(s) 626
BfaI CTAG 1 cut(s) 48
BfuAI ACCTGC 1 cut(s) 338
BisI GCNGC 3 cut(s) 477, 515, 585
BlsI GCNGC 3 cut(s) 478, 516, 586
Bme1390I CCNGG 1 cut(s) 519
Bme18I GGWCC 1 cut(s) 494
BmgT120I GGNCC 1 cut(s) 494
BmiI GGNNCC 3 cut(s) 138, 412, 608
BmrFI CCNGG 1 cut(s) 519
BmsI GCATC 2 cut(s) 463, 546
BplI GAGNNNNNCTC 2 cut(s) 159, 191
BpmI CTGGAG 1 cut(s) 582
BsaJI CCNNGG 1 cut(s) 380
BsaWI WCCGGW 1 cut(s) 407
Bse1I ACTGG 1 cut(s) 565
BseBI CCWGG 1 cut(s) 519
BseDI CCNNGG 1 cut(s) 380
BseGI GGATG 1 cut(s) 454
BseMII CTCAG 4 cut(s) 206, 237, 332, 555
BseNI ACTGG 1 cut(s) 565
BseRI GAGGAG 1 cut(s) 290
BseSI GKGCMC 1 cut(s) 139
BseXI GCAGC 2 cut(s) 463, 526
BsgI GTGCAG 1 cut(s) 533
Bsh1236I CGCG 1 cut(s) 549
BshFI GGCC 2 cut(s) 60, 299
BshNI GGYRCC 1 cut(s) 136
BsiSI CCGG 1 cut(s) 408
BsmAI GTCTC 1 cut(s) 626
BsnI GGCC 2 cut(s) 60, 299
Bsp1286I GDGCHC 1 cut(s) 139
Bsp143I GATC 1 cut(s) 106
Bsp19I CCATGG 1 cut(s) 380
BspACI CCGC 2 cut(s) 415, 584
BspANI GGCC 2 cut(s) 60, 299
BspCNI CTCAG 5 cut(s) 207, 238, 331, 554, 651
BspFNI CGCG 1 cut(s) 549
BspLI GGNNCC 3 cut(s) 138, 412, 608
BspMI ACCTGC 1 cut(s) 338
BspPI GGATC 1 cut(s) 101
BspT107I GGYRCC 1 cut(s) 136
BsrI ACTGG 1 cut(s) 565
BssECI CCNNGG 1 cut(s) 380
BssMI GATC 1 cut(s) 106
BssT1I CCWWGG 1 cut(s) 380
Bst2UI CCWGG 1 cut(s) 519
Bst4CI ACNGT 1 cut(s) 82
Bst6I CTCTTC 1 cut(s) 234
BstC8I GCNNGC 2 cut(s) 426, 547
BstDEI CTNAG 5 cut(s) 215, 246, 318, 541, 638
BstDSI CCRYGG 1 cut(s) 380
BstF5I GGATG 1 cut(s) 454
BstFNI CGCG 1 cut(s) 549
BstKTI GATC 1 cut(s) 109
BstMAI GTCTC 1 cut(s) 626
BstMBI GATC 1 cut(s) 106
BstMWI GCNNNNNNNGC 1 cut(s) 555
BstNI CCWGG 1 cut(s) 519
BstSCI CCNGG 1 cut(s) 517
BstSLI GKGCMC 1 cut(s) 139
BstUI CGCG 1 cut(s) 549
BstV1I GCAGC 2 cut(s) 463, 526
BsuRI GGCC 2 cut(s) 60, 299
BtgI CCRYGG 1 cut(s) 380
BtsCI GGATG 1 cut(s) 454
BtsIMutI CAGTG 3 cut(s) 86, 258, 558
BveI ACCTGC 1 cut(s) 338
Cac8I GCNNGC 2 cut(s) 426, 547
Cfr13I GGNCC 1 cut(s) 494
Csp6I GTAC 1 cut(s) 387
CviAII CATG 3 cut(s) 341, 381, 554
CviQI GTAC 1 cut(s) 387
DdeI CTNAG 5 cut(s) 215, 246, 318, 541, 638
DpnI GATC 1 cut(s) 108
DpnII GATC 1 cut(s) 106
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 380
Eco47I GGWCC 1 cut(s) 494
EcoRII CCWGG 1 cut(s) 517
EcoT14I CCWWGG 1 cut(s) 380
ErhI CCWWGG 1 cut(s) 380
FaeI CATG 3 cut(s) 344, 384, 557
FaiI YATR 9 cut(s) 123, 196, 342, 382, 464, 555, 600, 602, 620
FatI CATG 3 cut(s) 340, 380, 553
FauI CCCGC 1 cut(s) 422
Fnu4HI GCNGC 3 cut(s) 477, 515, 585
FokI GGATG 1 cut(s) 441
Fsp4HI GCNGC 3 cut(s) 477, 515, 585
FspBI CTAG 1 cut(s) 48
GluI GCNGC 3 cut(s) 477, 515, 585
GsuI CTGGAG 1 cut(s) 582
HaeIII GGCC 2 cut(s) 60, 299
HapII CCGG 1 cut(s) 408
Hin1II CATG 3 cut(s) 344, 384, 557
HinfI GANTC 5 cut(s) 4, 242, 248, 327, 634
HpaII CCGG 1 cut(s) 408
HphI GGTGA 1 cut(s) 533
Hpy188I TCNGA 5 cut(s) 211, 247, 354, 627, 641
Hpy188III TCNNGA 2 cut(s) 104, 230
HpyAV CCTTC 2 cut(s) 24, 568
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 1 cut(s) 615
HpyCH4V TGCA 4 cut(s) 97, 347, 392, 514
HpyF10VI GCNNNNNNNGC 1 cut(s) 555
HpyF3I CTNAG 5 cut(s) 215, 246, 318, 541, 638
HpySE526I ACGT 1 cut(s) 615
Hsp92II CATG 3 cut(s) 344, 384, 557
Kzo9I GATC 1 cut(s) 106
LmnI GCTCC 3 cut(s) 44, 484, 534
Lsp1109I GCAGC 2 cut(s) 463, 526
LweI GCATC 2 cut(s) 463, 546
MaeI CTAG 1 cut(s) 48
MaeII ACGT 1 cut(s) 615
MalI GATC 1 cut(s) 108
MboI GATC 1 cut(s) 106
MboII GAAGA 4 cut(s) 232, 251, 275, 480
MhlI GDGCHC 1 cut(s) 139
MluCI AATT 3 cut(s) 73, 293, 578
MluI ACGCGT 1 cut(s) 547
MlyI GAGTC 3 cut(s) 251, 257, 628
MnlI CCTC 3 cut(s) 5, 210, 268
MroXI GAANNNNTTC 1 cut(s) 74
MseI TTAA 1 cut(s) 644
MspA1I CMGCKG 1 cut(s) 417
MspI CCGG 1 cut(s) 408
MspR9I CCNGG 1 cut(s) 519
MvaI CCWGG 1 cut(s) 519
MvnI CGCG 1 cut(s) 549
MwoI GCNNNNNNNGC 1 cut(s) 555
NcoI CCATGG 1 cut(s) 380
NdeII GATC 1 cut(s) 106
NlaIII CATG 3 cut(s) 344, 384, 557
NlaIV GGNNCC 3 cut(s) 138, 412, 608
PdmI GAANNNNTTC 1 cut(s) 74
PfeI GAWTC 2 cut(s) 4, 327
PkrI GCNGC 3 cut(s) 478, 516, 586
PleI GAGTC 3 cut(s) 250, 256, 628
PpsI GAGTC 3 cut(s) 250, 256, 628
Psp1406I AACGTT 1 cut(s) 615
Psp6I CCWGG 1 cut(s) 517
PspGI CCWGG 1 cut(s) 517
PspN4I GGNNCC 3 cut(s) 138, 412, 608
PspPI GGNCC 1 cut(s) 494
RsaI GTAC 1 cut(s) 388
RsaNI GTAC 1 cut(s) 387
SaqAI TTAA 1 cut(s) 644
SatI GCNGC 3 cut(s) 477, 515, 585
Sau3AI GATC 1 cut(s) 106
Sau96I GGNCC 1 cut(s) 494
SchI GAGTC 3 cut(s) 251, 257, 628
ScrFI CCNGG 1 cut(s) 519
SduI GDGCHC 1 cut(s) 139
SetI ASST 9 cut(s) 49, 130, 313, 352, 478, 513, 530, 618, 650
SfaNI GCATC 2 cut(s) 463, 546
SinI GGWCC 1 cut(s) 494
Sse9I AATT 3 cut(s) 73, 293, 578
SsiI CCGC 2 cut(s) 415, 584
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 517
StyI CCWWGG 1 cut(s) 380
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 1 cut(s) 618
TasI AATT 3 cut(s) 73, 293, 578
TauI GCSGC 1 cut(s) 587
TfiI GAWTC 2 cut(s) 4, 327
Tru1I TTAA 1 cut(s) 644
Tru9I TTAA 1 cut(s) 644
TscAI CASTG 3 cut(s) 93, 258, 565
TseI GCWGC 2 cut(s) 476, 514
TspDTI ATGAA 5 cut(s) 138, 252, 296, 479, 542
TspGWI ACGGA 1 cut(s) 347
TspRI CASTG 3 cut(s) 93, 258, 565
VpaK11BI GGWCC 1 cut(s) 494
XmnI GAANNNNTTC 1 cut(s) 74
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.